91 results about "Diphtheria" patented technology

The present invention is in the field of pneumococcal capsular saccharide conjugate vaccines. Specifically, a multivalent Streptococcus pneumoniae immunogenic composition is provided with various conjugated capsular saccharides from different S. pneumoniae serotypes conjugated to 2 or more different carrier proteins, where the composition comprises serotype 19F capsular saccharide conjugated to diphtheria toxoid (DT) or CRM197, optionally wherein 19F is the only saccharide in the composition conjugated to diphtheria toxoid (DT) or CRM197.

The present invention provides novel methods of producing diphtheria toxin. In particular, the present invention provides novel methods of producing nontoxic forms of diphtheria toxin, e.g., CRM197. The present invention also provides novel compositions comprising diphtheria toxin or nontoxic forms of diphtheria toxin, e.g., CRM197.

This invention discloses a method for preparing recombinant diphtherin and its application. The recombinant diphtherin comprises diphtherin DAB389, a linker peptide (Gly4Ser) 2, and alpha-melanocyte stimulating hormone. The method comprises: (1) preparing a gene fragment of DAB389(Gly4Ser)2-alpha-MSH and a leading peptide, constructing an expression plasmid containing the gene fragment, and transforming Escherichia coli; (2) inducing with IPTG to obtain the target protein, purifying, digesting with Xa factor, and separating to obtain DAB389(Gly4Ser)2-alpha-MSH fusion protein. The method has such advantages as easy purification, and high yield. The recombinant diphtherin has high activity, and can attack and kill malignant melanoma without injuring normal cells, thus can be used to treat malignant melanoma.

The present invention provides compositions and methods for inhibiting abnormal cell growth. In particular, the invention provides nucleic acids encoding Diphtheria toxin fusion proteins comprising residues 1-388 of Diphtheria toxin, wherein the native furin cleavage site has been substituted for a matrix metalloproteinase or plasminogen activator cleavage site, and a heterologous polypeptide and the polypeptides encoded by such nucleic acids. In addition, the invention provides methods of treating cancer by administering such polypeptides.

The invention discloses a post-neurosurgical intracranial bacterial infection common pathogenic bacterium MLPA detection probe and an application thereof. The probe comprises a first group of probes to a seventeenth group of probes, and the first group of probes to the seventeenth group of probes are probes for detecting Neisseria meningitides, Bacillus pyocyaneus, Salmonella, Stenotrophomonas maltophilia, Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella pneumonia, Serratia marcescens, Acinetobacter lwoffii, Corynebacteria diphtheria, Streptococcus mitis, Acinetobacter baumannii, Streptococcus pneumonia, Propionibacterium acnes, Listeria monocytogenes, Enterobacter cloacae and Haemophilus influenza sequentially. The probe is developed through an MLPA technology, can rapidly diagnose post-neurosurgical intracranial bacterial infection 17 common pathogenic bacteria once, provides possibility for rapid diagnosis of pathogenic bacteria of like patients, and is helpful for reducing the fatality and the disability rate of like infection.

The invention discloses anti-bacterium hand cream made of traditional Chinese medicines, which belongs to the field of domestic chemistry engineering and consists of a component A and a component B. The component A includes traditional Chinese medicine powder, the component B includes cream matrix, and the component A and the component B are mixed according to mass ratio of 1:100, so that the anti-bacterium hand cream is obtained. The anti-bacterium hand cream has the advantages that the anti-bacterium hand cream not only can effectively nourish skins of a user, improves the quality of the skins and is harmless to the skins, but also can suppress hepatitis B virus, and has higher killing effects for cocci of viral influenza, dysentery, pneumonia, diphtheria and meningitis.

The invention discloses an establishing method of a scrophularia ningpoensis tissue culture system. Scruphularia ningpoensis has effects of clearing heat and purging pathogenic fire, nourshing yin to promote salivation and treating heat pain and yin impairement, damp toxin and spotting, body fluid impairment constipation, eye-throat soreness, diphtheria and pain, swelling and sore-toxin. Therefore, planting scrophularia ningpoensis has good development prospect, and scrophularia ningpoensis is a good economic plant. Plant tissue culture technology is utilized to improve breeding speed and scale of scrophularia ningpoensis, so that industrialized production of scrophularia ningpoensis seedlings is realized. Stems with buds are used as explants, scrophularia ningpoensis tissue culture regenerated plants are successfully obtained through steps of inducing, breeding, rooting and acclimatization and transplanting, and a systematic scrophularia ningpoensis tissue culture plant regeneration system is established. The establishing method has the advantages of simple technique, easiness in operation and quick material regeneration.

The invention provides a packaging cell line for production of diphtheria toxin (DT) expressing, non-replicating adenovirus for use in suicide gene therapy of cancer cells, as well as production of immunotoxins. Also provided are methods for producing diphtheria toxin (DT) expressing, non-replicating adenovirus, methods for producing immunotoxins, and adenovirus and immunotoxins produced by those methods. Further provided are methods for making a cell resistant to diphtheria toxin.

The invention discloses cationic phthalocyanine. A structural formula is shown in the figure. The cationic phthalocyanine derivative disclosed by the invention has good water solubility and low aggregation degree, has good photosensitive activity and antitumor activity, and is applicable to being used as a photosensitizer for diphtheria/pertussis/tetanus (DPT) treatment. The invention also relates to a preparation method and an application of the cationic phthalocyanine.

The invention discloses a dendritic toxin mutant CRM197 coding sequence, the nucleotide sequence of which is optimized. The synthesized coding sequence is ligated into an expression vector pET32a(+) after double restriction endonuclease digestion, and soluble highly expressed recombinant CRM197 is obtained in Escherichia coli BL21(DE3). Compared with diphtheria toxin, the recombinant CRM197 protein prepared according to the invention is safe and non-toxic and can induce high titer protective antibody in mice, and has broad application prospect in large-scale and efficient preparation of recombinant CRM197.

The invention discloses an air refreshing agent. The air refreshing agent is prepared from the following raw materials in parts by weight: 5 to 10 parts of purple perilla, 5 to 10 parts of honeysuckle, 3 to 8 parts of artemisia vulgaris, 3 to 5 parts of lilac, 3 to 5 parts of flos magnoliae liliflorae, 5 to 10 parts of eupatorium fortune, 3 to 8 parts of mint and 3 to 8 parts of fructus forsythia. According to the air refreshing agent disclosed by the invention, the formula is designed and the proportion is screened by combining modern pharmacological research on the basis of a traditional Chinese medicine theory; the active ingredients of the air refreshing agent can refresh air, also has an inhibiting effect on extrinsic streptococcocci, typhoid bacilli, corynebacterium diphtheria, escherichia coli, vibrio cholera and the like, and can prevent the diseases, such as cold, pneumonia and enteritis.

The present invention relates to the field of vaccines for protecting against polio, and in particular to combination vaccines for protecting against polio, diphtheria, tetanus, and pertussis diseases. Specifically, vaccines comprising reduced dose inactivated poliovirus (IPV) is provided, which can maintain an adequate or improved level of protection against polio.

The invention discloses a Chinese herbal medicine feed additive for beef cattle and a preparation method thereof. The Chinese herbal medicine feed additive is prepared from the following components in parts by weight: 10 to 20 parts of radix astragali seu hedysari, 5 to 15 parts of radix codonopsis, 1 to 3 parts of radix gynurae bodinieri, 1 to 2 parts of rhizoma imperatae, 10 to 20 parts of cortex magnoliae officinalis, 5 to 15 parts of radix dipsaci, 5 to 15 parts of herba eupatorii, 15 to 30 parts of radix notoginseng and 10 to 20 parts of bulbus lilii. A compound traditional Chinese medicine preparation for enhancing the growth performance and the immunological function of the beef cattle has the functions of resisting viruses, decreasing blood sugar, resisting bacteria, diminishing inflammation, regulating immune, resisting injury, resisting mutation and the like, and is capable of inhibiting cell apoptosis and increasing the survival rate of lymphocyte; meanwhile, the compound traditional Chinese medicine preparation has an inhibiting effect on staphylococcus aureus, corynebacterium diphtheria, bacillus subtilis, typhoid bacillus, paratyphoid bacteria, escherichia coli and the like; a good effect in aspect of enhancing the immunity and the growth ability of the beef cattle is shown.