19 results about "Listeria" patented technology

The invention discloses a preparation method of ocimum gratissimum carbon dots and application of the ocimum gratissimum carbon dots in antibacterial drugs and food preservation. The preparation method of the ocimum gratissimum carbon dots comprises the following steps: performing steam distillation on ocimum gratissimum leaves to remove essential oil, drying in the shade, and crushing; adding an alcohol solvent into the powder, extracting, filtering, concentrating and drying to obtain an alcohol extract; dispersing the alcohol extract in distilled water, and stirring and pyrolyzing on a heating table; and diluting the pyrolysis product with distilled water, centrifuging, taking supernate, filtering with a filter membrane, dialyzing, and freeze-drying to obtain the carbon dots. The obtained carbon dots have a carbon dot-vesicle coexisting structure, and the average particle size is 5.732 + / -0.5 nm. The prepared ocimum gratissimum carbon dots can be applied to preparation of antibacterial drugs, especially drugs for inhibiting the activity of bacillus cereus and listeria, and the field of food preservation, and have the advantages of being wide in raw material source, simple in preparation method, low in cost, environmentally friendly and the like.

The invention provides a listeria monocytogenes detection kit based on iron-tannic acid network coating aggregation-induced emission nanodots and a detection method. The listeria monocytogenes detection kit comprises Cas12a protein, crRNA, a single-stranded nucleic acid fluorescent probe, an iron-tannic acid network coating aggregation-induced emission nanodot dispersion liquid, a Lamp element, a buffer solution and enzyme-free water. According to the present invention, the iron-tannic acid network coating aggregation-induced emission nano-dot is adopted as the light-emitting material, Cy5-ssDNA is adopted as the fluorescent probe, and the specific gene segment Listeria of Listeria monocytogenes is adopted as the target sequence to construct the two-color ratio fluorescent biosensor, and the two-color ratio fluorescent biosensor can be used for detection of Listeria monocytogenes in food, the method has the advantages of simplicity, no dependence on large instruments, low cost, short detection period, low background, high sensitivity, strong specificity, high efficiency, reliability and the like.

The invention discloses a Plusteria monocytogenes strain capable of reducing antibiotic resistance genes and promoting growth and application of the Plusteria monocytogenes strain. The strain is separated from rice soil, and is identified as Plusteria based on physiological and biochemical characteristics and 16S rRNA (ribosomal Ribonucleic Acid) analysis. The Plusteria bacteria provided by the invention have a remarkable reduction effect on antibiotic resistance genes in the organic fertilizer; pot experiment results show that the strain can effectively reduce abundance of antibiotic resistance genes at the upper part and the root part of the lettuce and can significantly promote growth of the lettuce.

The application discloses a kind of smelly danchang dots and preparation method and application, belong to medical technical field.The preparation method of smelly danchang dot includes: smelly danchang fine powder is dispersed in distilled water, and is stirred pyrolysis on heating table;Pyrolysis product is diluted with distilled water, and supernatant is obtained by centrifugation, filter membrane filtration, dialysis, freeze-drying, to obtain carbon dot.The average particle size of obtained carbon dot is 4.51±1.04nm.The smelly danchang dot prepared in the application shows strong inhibitory activity to a variety of gram-positive bacteria (such as listeria, methicillin-resistant staphylococcus aureus MRSA) and fungi (such as Candida albicans), especially to clinically tricky drug-resistant strains, such as vancomycin-resistant enterococci (VRE) and MRSA effective, can be applied to the preparation of antibacterial drugs, antibacterial dressing, disinfectant or preservative, with the advantages of wide raw material source, simple preparation method, low cost, environment-friendly and the like.

A method for preparing a refrigerated, pre-cooked egg based product is disclosed. The method includes combining a liquid egg material with an antibacterial composition comprising nisin, citric acid, and an secondary organic acid having a pKa of from about 3.5 to about 8 to form a liquid egg formulation with surprisingly good control of Listeria growth.

The present application relates to the technical field of antibacterial peptides, in particular to IPC C07K7, more particularly to a gingival protection antibacterial peptide or its stereoisomer and a preparation method and application thereof.The present application provides a gingival protection antibacterial peptide or its stereoisomer, which comprises antibacterial peptide MOAMPV14R; the antibacterial peptide MOAMPV14R comprises a structure shown in formula 1.In the present application, a new antibacterial peptide MOAMPV14R is designed and synthesized, which can effectively inhibit the activity of Streptococcus mutans, Porphyromonas gingivalis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Listeria, and M. maltophilia, and the MIC value is 1-8 μg / mL, the antibacterial effect is excellent, the hemolytic activity and cytotoxicity are low, and the safety is good.

The invention relates to a preparation method and application of acetylated raspberry ketone, and the preparation method is characterized in that acetic anhydride is used as an acetylation reagent to realize hydroxyl acetylation of raspberry ketone in the presence of a proper catalyst and a proper solvent so as to obtain high-purity acetylated raspberry ketone. The reaction conditions are mild, and high temperature and high pressure are not needed; the product purity is high; the method is simple to operate and suitable for industrial production. Moreover, when the acetylated raspberry ketone is used for preparing an antimicrobial composition, the effect is remarkable, and microorganisms comprise Listeria bacteria or salmonella typhimurium.

The invention provides a primer and a probe group for detecting sheep abortion-causing bacteria and application of the primer and the probe group, and belongs to the technical field of biological detection. The primer and probe group is characterized in that the sequence of the primer and probe group for detecting the listeria is shown as SEQ ID NO.1-3, and the sequence of the probe group for detecting the listeria is shown as SEQ ID NO.2-3; according to the primer for detecting the salmonella abortus, the sequence of the probe group is as shown in SEQ ID NO.4-6; the sequences of the primer and the probe group aiming at Brucella detection are shown as SEQ ID NO.7-9; the sequence of a primer and a probe group for detecting chlamydia psittaci is as shown in SEQ ID NO.10-12. The invention further discloses a kit for detecting chlamydia psittaci. The method has the characteristics of visualization, high sensitivity, strong specificity, simplicity and convenience in operation, short time consumption and the like, realizes rapid differential diagnosis of four sheep bacterial abortion diseases through one-time PCR amplification, and has important advantages in the field of sheep disease diagnosis and identification.

Described herein is an extract of a herbal composition comprising at least two different dry plants, the compounds are useful as antimicrobial and / or antibiotic film agents in the treatment or prevention of microbial infections caused by bacteria (e.g., Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, Streptococcus, or Staphylococcus) or by fungi, as well as in the treatment or prevention of microbial infections caused by bacteria (e.g., Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, Streptococcus, or Staphylococcus). It has been found that in such an extract, the active ingredients exert their biological effects in a synergistic manner. The extracts may form active ingredients of food supplements, nutraceuticals, pharmaceutical or cosmetic compositions or functional foods or food additives. Also described herein are methods for preparing the extracts.

Embodiments of the present disclosure generally relate to compositions of a lyophilized (freeze dried) Listeria strain based therapeutics and a method for lyophilizing compositions of the therapeutics. A Listeria monocytogenes (LM) based vaccine formulation contains a lyophilized powder including a LM bacteria, the LM bacteria comprising at least one therapeutic agent and a lyophilization medium and a delivery composition. A method of forming a Listeria monocytogenes (LM) based vaccine formulation is disclosed. The LM based vaccine formulation includes resuspending an LM stock in a lyophilization medium to form a lyophilization composition, wherein the lyophilization medium comprises at least a lyoprotectant and a buffer component, freezing the lyophilization composition, lyophilizing the lyophilization composition, and forming a lyophilized powder, the lyophilized powder contains a LM bacteria, a lyoprotectant, and a buffer component.

The invention discloses a novel broad-spectrum antibacterial peptide and application thereof, the novel broad-spectrum antibacterial peptide is a polypeptide obtained by 3-5 times of recombination and series connection of an amino acid sequence SEQ ID NO.1, or a chimeric peptide formed by connecting the amino acid sequence SEQ ID NO.1 and a cell penetrating peptide containing 2-10 arginine, and the chimeric peptide is named TN3. The antibacterial peptide has certain broad-spectrum antibacterial activity, and has an obvious antibacterial effect on staphylococcus aureus, escherichia coli, listeria monocytogenes and pseudomonas fluorescens. In addition, the broad-spectrum antibacterial peptide is low in cytotoxicity, has good biocompatibility and has clinical application potential.

The invention discloses laggera pterodonta carbon dots as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method of the laggera pterodonta carbon dots comprises the following steps: dispersing laggera pterodonta fine powder in distilled water, and stirring and pyrolyzing on a heating table; and diluting the pyrolysis product with distilled water, centrifuging, taking supernate, filtering with a filter membrane, dialyzing, and freeze-drying to obtain the carbon dots. The average particle size of the obtained carbon dots is 4.51 + / -1.04 nm. The laggera pterodonta carbon dots prepared by the invention show strong inhibitory activity on various gram-positive bacteria (such as Listeria monocytogenes and methicillin-resistant staphylococcus aureus MRSA) and fungi (such as Candida albicans), and are particularly effective on clinically-difficult drug-resistant strains, such as vancomycin-resistant enterococcus (VRE) and MRSA; the compound can be applied to preparation of novel antibacterial drugs, antibacterial dressings, disinfectants or preservatives, and has the advantages of wide raw material sources, simple preparation method, low cost, environmental friendliness and the like.

The invention relates to the technical field of pet food, in particular to natural antibacterial wet food for pets and a preparation method thereof.The natural antibacterial wet food for pets comprises fermented pea protein, fresh meat / fish, fermented hibiscus cannabinus seed protein and the like, and antibacterial and fresh-keeping effects are achieved through lactic acid bacteria fermentation products and microcapsule herbal compounds; the formula also contains organic acid, a natural chelating agent, vitamins, cranberry extract and the like, the total water content reaches 75-78%, lactobacillus plantarum produced by pea protein under the action of lactobacillus plantarum can destroy cell membrane structures of various gram-positive bacteria, and pediococcus produced by pediococcus has a specific inhibition effect on pathogenic bacteria such as listeria monocytogenes. Through the synergistic effect of the two types of bacteriocin, the antibacterial spectrum is expanded, and respective antibacterial blind areas are filled up.

A hybridoma cell line secreting an ActA monoclonal antibody and a use thereof. The hybridoma cell line was deposited on Jul. 8, 2021, at the China Center for Type Culture Collection (CCTCC) with the depository accession number of CCTCC NO: C2021174. The present invention also discloses the ActA monoclonal antibody secreted by this hybridoma cell line or its progeny cell line, a detection kit containing this ActA monoclonal antibody, immunomagnetic beads, the preparation method and use of immunomagnetic beads, and competitive ELISA and indirect ELISA detection methods. The ActA monoclonal antibody of the present invention has the advantages of high titer, good specificity, and strong affinity with the natural antigen. The Listeria competitive ELISA detection kit and immunomagnetic beads developed based on this antibody have high sensitivity and good stability, effectively monitoring the level of ActA antibodies in clinical serum samples, and can be used for labeling Listeria monocytogenes.

Disclosed herein are methods for producing a mutant bacteriophage having an altered range of hosts. In addition, the present application discloses methods and systems for the rapid detection of microorganisms in a sample, such as a plurality of species of the genus Listeria. Also disclosed is a genetically modified bacteriophage comprising an indicator gene in an advanced gene region. The specificity of a bacteriophage, such as a Listeria specific bacteriophage, allows the detection of a particular microorganism, such as a variety of species of Listeria, and the indicator signal can be amplified to optimize assay sensitivity.

This disclosure relates to a method for sanitizing a space. The method includes applying a composition containing triethylene glycol into a space containing a pathogen (e.g., a Staphylococcus, a Pseudomonas, a Listeria, Salmonella, Klebsiella, a mycobacterium, a mold, or a spore) in an amount effective to inactivate the pathogen.

The invention provides application of ubiquitin specific protease 33 in regulation and control of polarization of macrophages and anti-tumor and / or anti-infection, and belongs to the technical field of biological medicine manufacturing. The invention discloses ubiquitin specific protease 33 serving as a new immune activation target to participate in polarization of M1 type macrophages. The invention reveals that USP33 regulates and controls the protein stability of the IRF2 factor through a ubiquitination modification way for the first time, and further regulates and controls the expression of downstream inflammatory cytokines such as INF-gamma. The USP33 is knocked out or knocked down to degrade the IRF2, so that the inhibition effect of the IRF2 on a proinflammatory signal is relieved, the polarization of macrophages to M1 type is promoted, the expression of proinflammatory factors is up-regulated, the proliferation of tumor cells is inhibited, and the growth of tumors is remarkably inhibited; and meanwhile, the defense capability of the host to the Listeria monocytogenes can be enhanced by virtue of myeloid specific knockout of the USP33.

The invention relates to a method of making a 1,2-dioxetane, the method comprising reacting a naphthalene endo peroxide (NEPO) with the double bond of an enol ether to form the 1,2-dioxetane. The 1,2-dioxetanes are of great commercial interest for a variety of applications, such as singlet oxygen detection, enzyme detection and pathogen (e.g., Salmonella spp. and Listeria spp.) detection.

Embodiments of the present disclosure pertain to a modified Listeria monocytogenes bacterium (Listeria) that is associated with at least one therapeutic agent. The therapeutic agent may be associated with a surface of the Listeria through a Listeria binding agent. The therapeutic agent may also be covalently associated with the surface of the Listeria through a cleavable linker that directly links the therapeutic agent to the surface of the Listeria. Additional embodiments of the present disclosure pertain to methods of delivering at least one therapeutic agent to a subject by administering to the subject a Listeria of the present disclosure. Further embodiments of the present disclosure pertain to methods of making a Listeria of the present disclosure by associating the Listeria with at least one therapeutic agent.