717 results about "Ovis" patented technology

The invention relates to an early weaning meat sheep fattening compound biological feed additive, which belongs to the technical field of animals feeding by feed. The feed additive comprises prebiotics consisting of candida utilis, bacillus subtilis and a lactobacillus plantarum microbial agent, non-starch polysaccharide enzyme containing cellulase, xylanase, beta-dextranase, beta-mannase and pectinase and a yeast culture formed by using saccharomyces cerevisiae to completely ferment bean pulp. The additive is specially used for fattening period ration of the early weaning(the weaning lunar age is less than or equal to 2 ages of the moon) meat sheep, and the adding percentage for the full-mixing ration of the fast-fattening meat sheep in 3 to 5 ages of the moon is 1 percent. The additive can provide functional nutrients such as digestive enzyme, B group vitamins and growth promoting factors, and the like which are inadequately produced and insufficient for the early weaning baby sheep and simultaneously contains probiotics for adjusting the micro-ecological environment in intestinal canals, thereby improving the immunity and the healthy level of the baby meat sheep; and after weaning, the daily gaining in weight is more than 10 percent in the prior period and later period of fattening, and the economic benefits are obviously improved.

The present invention relates to a method to prepare a reagent kit to quickly diagnose mycobacterium tuberculosis antigens jointly packaged by 38KD and 16KD antigens and a test method. The blood antigen test paper of the present invention jointly packages 38 KD and 16KD of TB antigens into a lower part of a NC film to form a test line (T). Goat anti-mouse IgG is packaged into an upper part of the NC film to form a quality control line (C). In addition, anti-human IgG (mouse anti-human IgG, goat anti-human IgG or SPA) marks nanometer coloring particles (colloidal gold particles or sol particles or nanometer beads) to prepare a labeling pad. The NC film, the labeling pad, a sampling pad and a sample adsorption pad are glued and assembled onto a plastic board to form the test paper. During test, few tested sample is added onto the NC film of the test paper. And then, sample diluents are added through a sampling end. After sampling, it is necessary to observe result of immunoreaction, thus realizing quick auxiliary TB antigen diagnosis.

The invention discloses a method for preparing a sheep placenta extract and a sheep placenta hydrolyzed collagen concentrated solution, which belong to the technical field of utilization of sheep placentas. The method comprises the following steps of: preparing sheep placenta powder from commercially-available freeze-dried sheep placentas serving as a raw material; preparing a crude sheep placenta extract extracting solution; and preparing a sheep placenta extract and a sheep placenta hydrolyzed collagen concentrated solution product. The method has the advantages of easiness, easiness and convenience for operating, simple equipment, mild reaction conditions and saving in energy; by adopting the method, a plurality of products such as the sheep placenta hydrolyzed collagen concentrated solution, a feed additive and the like are prepared simultaneously while the sheep placenta extract is prepared, and comprehensive utilization of the sheep placentas is realized; in a production process, substance resources can be fully utilized, 'three wastes' are not discharged, and the environment is protected; and the production cost is low, and popularization and application are facilitated. The method can be widely used for preparing sheep placenta extract and the sheep placenta hydrolyzed collagen concentrated solution, and a product prepared by using the method can be widely applied in the industries of makeups, health-care products and the like.

The invention relates to an enzyme immunosensor based on chitosan and nano-gold, and by using the specific combination of the monoclonal antibody of the cell wall of mycobacterium trberculosis with mycobacterium trberculosis, the enzyme immunosensor is applicable to the rapid detection of mycobacterium trberculosis in milk. The characteristic is that the enzyme immunosensor for detecting mycobacterium tuberculosis is prepared by the following steps: modifying the surface of a glassy carbon electrode through electrochemical deposition method by a mixed solution of chitosan gel, nano-gold solution, and goat anti-mouse antibody labeled by alkaline phosphatase; and fixing the mycobacterium trberculosis monoclonal mouse antibody on the surface of the glassy carbon electrode modified by the goat anti-mouse-chitosan-nano-gold film which is labeled by alkaline phosphatase through the specific antigen-antibody reaction. Quantitative analysis is performed by detecting the electric signal changegenerated before and after the incubation reaction of mycobacterium trberculosis and the modified electrode through differential pulse voltammetry. The preparation method of the sensor is simple, andthe sensor has the advantages of high sensitivity, short detection time, and simple operation, and is applicable to the method for the rapid detection of mycobacterium trberculosis.

The invention discloses a method for breeding a new variety of Taihang black goats. In the breeding method, a three way cross among a Taihang black goat, a Jintang black goat and a Nubian is performed, and individual bulk selection is used for selective breeding and propagation to breed the new variety of Taihang black goats. In the breeding method of the invention, by introducing the advantageous genes associated with the large body size, high yield and high milk productivity of the Jintang black goats and Nubian goats are introduced, the bred new variety of the Taihang black goats, apart from the characteristics of high adaptively and complete black hair of the Taihang black goat, has the advantages of large body size, high growing speed, high meat production capacity, high milk productivity and the like. Compared with Taihang black goats, the new variety has the advantages that: the lambing percentage is as high as over 150 percent; the growing speed is high; the body size is so large as the weight of a mature male goat is over 55 kilograms and the weight of a female goat is over 40 kilograms; the meal productivity is high; the meat quality is high; the milk yield is high; and the adaptability is high. Thus, the method is suitable for drylot feeding and market lamb production development.

The invention relates to self-proportioned concentrate for goats. The self-proportioned concentrate comprises the following components in parts by weight: 30-60 parts of corn, 5-23 parts of vinasse, 10-20 parts of bean pulp, 5-14 parts of bran, 0-13 parts of alfalfa meal, 0-25 parts of grass meal, 0-2 parts of bone meal, 0.8-1.2 parts of mountain flour, 0-1.1 parts of salt, 0-0.35 part of urea, 0-1.1 parts of sodium bicarbonate and 0.1-3.3 parts of an additive. The concentrate comprises breeding sheep concentrate, fattening sheep concentrate and lamb concentrate. According to the concentrate, different raw materials and different ratios are selected according to the nutritional requirements of different sheep, and in the aspect of nutrition collocation, simple and feasible effects are achieved while comprehensive nutrition is ensured; according to the formula provided by the invention, the concentrate can effectively achieve good growth promoting and breeding effects, and the morbidity of sheep is effectively reduced; according to the formula, the feeding cost is reduced, the labor force is saved, agricultural and sideline products are applied to the goat feed, and the advantage of efficient utilization is achieved by reasonable collocation.

The invention relates to a test strip for detecting colibacillus O157:H7 mouse serum antibody by colloidal gold immunochromatography, belonging to the technical field of antibody detection kits and consisting of colloidal gold cushion prepared by utilizing a colloidal gold solution the granule of which is 20nm to mark purified sheep anti-mouse IgG, nitric acid cellulose films which are respectively coated by a colibacillus O157:H7 antigen and purified mouse serum IgG and serve as a detection line and a quality control line, a detection test strip formed by assembling a sample absorbing cushionprepared from glass cellulose films and an absorbing cushion made of water absorbing filter paper, positive serum, negative serum, a serum sample diluent and a test strip operating instruction. The test strip is convenient to use, rapid and sensitive, and a detection result can be obtained within 10 minutes, and therefore, the test strip is suitable for on-site detection when infection status ofmouse O157:H7 is surveyed and traced back in animal farmer areas, food production enterprise areas and the like and use when the food safety environment risk evaluation is carried out.

The invention belongs to the field of biological detection reagents and relates to a kit for indirect ELISA (Enzyme-Linked Immunosorbent Assay) detection of IgG or IgA antibodies of PRRSV (Porcine reproductive and respiratory syndrome virus) of pigs. The kit is used for indirect ELISA detection of the IgG or IgA antibodies of the PRRSV in serum or saliva of pigs and comprises (a) an antibody detection board, (b) an HRP-labeled goat anti-pig IgG antibody solution or HRP-labeled goat anti-pig IgA, (c) a positive control, namely PRRSV standard positive serum or saliva, (d) a negative control, namely PRRSV negative serum or saliva, (e) sample diluting liquid, (f) 10* concentrated washing liquid, (g) substrate developing liquid, and (h) stopping liquid. The kit is good in PRRSV specificity and sensitivity when being used for detection of PRRSV IgG or IgA antibodies in serum or saliva of pigs. A repeatability test also shows that the kit is good in repeatability.

The invention discloses a quick African swine fever virus detection card and application thereof, so as to solve the technical problem of hard African swine fever virus detection. Polyclonal antibodies PoAbI against African swine fever virus p30, P54 and p72 protein labeled with colloidal gold are absorbed on a colloidal gold labeling pad of the detection card; a detection membrane is provided with goat or rabbit anti-mouse IgG antibodies or a quality control line C printed by staphylococcus aureus SPA; and a detection line T printed by polyclonal antibodies PoAbII against African swine fevervirus p30, P54 and p72 protein is also arranged. The invention also discloses a using method for the quick African swine fever virus detection card. The method comprises steps: a to-be-detected sampleis acquired, and PBS or water is added for suspension or grinding; the sample is dripped to the sample loading end of the quick African swine fever virus detection card; and horizontal placing is carried out to observe a result. The quick African swine fever virus detection card adopts a double antibody sandwich method, and has the advantages of strong specificity, high sensitivity, high detection efficiency, high efficiency and practicability, and important practical application value.

The invention discloses a reagent card for colloidal gold immunochromatograohic assay of lily mottle virus and a preparation method of the reagent card for the colloidal gold immunochromatograohic assay of the lily mottle virus. The reagent card comprises a reagent card groove, a lining plate, a sample pad, a colloidal-gold combined pad, a nitrocellulose membrane and water-absorbing filtering paper, wherein the colloidal-gold combined pad contains a gold labeling probe; the lining plate is fixed in the reagent card groove; the sample pad, the colloidal-gold combined pad, the nitrocellulose membrane and the water-absorbing filtering paper are arranged connected to the upper surface of the lining plate in sequence; the nitrocellulose membrane is provided with a detection line and a control line which are respectively coated with rabbit-anti-LMoV polyclonal antibodies and goat-anti-rabbit IgG purified antibodies. The preparation method comprises the main steps: coating an immunochromatograohic membrane with the rabbit-anti-LMoV polyclonal antibodies, and preparing the colloidal-gold combined pad by using the immunochromatograohic membrane. The reagent card disclosed by the invention is fast to detect, high in accuracy, strong in specificity, easy to operate and free from need of special equipment and instruments.

The invention discloses combined detection test paper of influenza A virus antigen and influenza B virus antigen and a preparation method thereof. The test paper comprises a sample pad, a fiberglass membrane containing a colloidal gold particle label, a nitrocellulose membrane and water absorbing paper, wherein the nitrocellulose membrane comprises a detection area which is coated with an influenza A virus antibody, a detection area which is coated with an influenza B virus antibody and a control area which is coated with an goat anti-rabbit antibody; the colloidal gold particle label comprises a micro signal amplification system and a colloidal gold labeled rabbit IgG antibody; and the micro signal amplification system is a colloidal gold particle-avidin-biotin-influenza A/B virus antibody. According to the invention, an avidin-biotin microsignal amplification system is added in a double-antibody sandwich detection system, the signal of a target antibody is enlarged, the detection sensitivity is increased, false negative or detection omission due to weak signals can be avoided, simultaneously combined detection can be carried out on the influenza A and B virus antigens, and the detection time, sample and cost can be saved.

The invention provides primers for triple PCR of three types of sheep pathogenic mycoplasmas and a detection method. The three pairs of special primers MO, MCC and MCCP are synthesized respectively according to the designs of sheep mycoplasma pneumoniae, a goat mycoplasma mycoide subspecies and a goat mycoplasma pneumonia subspecies, and a triple PCR optimum reaction system and reaction conditions for the three types of sheep pathogenic mycoplasmas are provided. The simultaneous pathogenic detection of the sheep mycoplasma pneumoniae, the goat mycoplasma mycoide subspecies and the goat mycoplasma pneumonia subspecies can be rapidly carried out without cloning, sequencing and sequence comparison, and the detection method has the advantages of being rapid, accurate, strong in specificity, good in repeatability and the like, suitable for rapid detection of the sheep mycoplasma pneumoniae, the goat mycoplasma mycoide subspecies and the goat mycoplasma pneumonia subspecies and large-scale epidemiological investigation and has great economic and social benefits.

The invention discloses a method for breeding a new strain for Yuxi fatty-tailed mutton. The method breeds the new strain for the Yuxi fatty-tailed mutton by performing three-way cross of Yuxi fatty-tailed sheep, Borderdale sheep and Dorper sheep and performing selective breeding and propagation with an individual phenotypic selection method and a trait detection technique for meat. The new strain for the Yuxi fatty-tailed mutton bred by the breeding method keeps the advantage of the Yuxi fatty-tailed sheep, and has excellent wool characteristic of the Borderdale sheep and ideal meat physical characteristic of the Dorper sheep. Compared with the Yuxi fatty-tailed sheep, the new strain has the advantages of high lambing rate which can reach over 150 percent, high growth and development, large physique (the weight of the adult male sheep can reach over 55 kilograms and the weight of the female sheep can reach over 40 kilograms), high meat yield, good meat quality and strong adaptability, and is suitable for barn feeding and development of fat lamb production.

The present invention relates to methods of using m-tyrosine compounds from Festuca species for inhibiting weed growth and enhancing growth of non-weed plants. The present invention also relates to methods of identifying plants having herbicidal properties.

The invention relates to a dengue virus antibody enzyme-linked immunity diagnostic kit. The kit includes an enzyme labeling board, a negative control serum, a positive control serum, an enzyme marker, a sample diluent, a concentrated washing liquid, a substrate chromogenic liquid, a stop solution and a sealing plate glue, wherein the enzyme marker contains a goat anti-human IgG-HRP; an envelope antigen on the enzyme labeling board includes: a recombinant dengue virus type 1 envelope protein specific antigen with the amino acid sequence of SEQ ID No.1; a recombinant dengue virus type 2 envelope protein specific antigen with the amino acid sequence of SEQ ID No.3; a recombinant dengue virus type 3 envelope protein specific antigen with the amino acid sequence of SEQ ID No.5; and a recombinant dengue virus type 4 envelope protein specific antigen with the amino acid sequences of SEQ ID No .7. Early diagnoses can be made to patients infected with dengue fever for the second time or more than two times and serum epidemiological investigation to healthy people can be conducted through detecting dengue virus IgG antibodies in serum. Final diagnoses can be made to patients infected with dengue fever for the first time.

The invention discloses a fluorescence immunity chromatography test strip for escherichia coli O157:H7 detection. The test strip is prepared by a method comprising the following steps: 1) preparing water-soluble CdTe/CdS quantum dots with a core-shell structure; 2) marking escherichia coli O157:H7 rabbit polyclonal antibodies with the CdTe/CdS quantum dots; and 3) coating the escherichia coli O157:H7 marked rabbit polyclonal antibodies on a glass cellulose membrane to form a combination pad; respectively coating the escherichia coli O157:H7 polyclonal antibodies and goat anti-rabbit IgG on a cellulose nitrate membrane to form a detection line and a quality control line; and assembling a sample pad, the combination pad, the cellulose nitrate membrane and a piece of absorbent paper on a pvc base plate to form the fluorescence immunity chromatography test strip. The fluorescence immunity chromatography test strip has advantages of fast detection, high sensitivity, strong singularity and good stability in escherichia coli O157:H7 detection.

The invention provides a method for identifying tortoise-shell glue, deer-horn glue and products thereof. The method provided by the invention comprises the step of detecting by using the difference between the specific nucleotide sequence or amino acid sequence of genomes of the tortoise-shell glue, deer-horn glue and products thereof and the specific nucleotide sequence or amino acid sequence of genomes of other imitations, wherein the specific amino acid sequence is shown in SEQ. ID No1. According to the method provided by the invention, fake ingredients, such as donkeys, horses, cattle, pigs or sheep, in the tortoise-shell glue, deer-horn glue and products thereof can be detected rapidly, so as to distinguish the authenticity. The invention further relates a polypeptide and a composition formed by the polypeptide and a corresponding carrier. Furthermore, the invention relates to application of the polypeptide and composition thereof in detection of donkey, horse, cattle, pig or sheep-derived ingredients in the tortoise-shell glue and products thereof.

A sheep placenta extract in the form of oral liquid is prepared from sheep placenta through freeze-drying at -40-0 deg.C, pulverizing, extracting, sieving, and sterilizing.

A robotic injection system is herein described for delivering vaccines, reproductive hormones, and liquid materials to domestic herd animals. The robotic injection system includes a cooling-unit for storage of the liquid materials to be injected, a series of automatic gates to control the movement of herd animals, an RFID and camera ID reading system utilized for tracking identification numbers and medical history, a robotic arm to position and apply force in the injection process, and an injection mechanism for delivering injections to the patient. A streamlined system is described in delivering necessary injections to a mass number of domestic herd animals. A robotic injection system for injecting an accurate dosage of more than one fluid is described. For this description, bovines will be used as the primary example but this described invention also applies to other domestic herd animals such as sus, equus caballus, ovis aries, and capra aegagrus hircus.

The invention discloses a double-stained kit for auxiliary diagnosis of benign or malignant hepatocellular tumor and application thereof. The kit comprises the following components: mixed primary antibody of Arginase-1 and Glypian-3, mixed second antibody of alkaline phosphatase conjugated goat anti-rabbit second antibody and horse radish peroxidase labeled goat anti -mouse second antibody, chromogenic reagent AP-Red and DAB (Diaminobenzidine), and TBS (Tris buffer saline) buffer solution. Two immunohistochemical labels provided by the invention are used for auxiliary diagnosis of benign or malignant hepatocellular tumor and differentiation degrees of tumor, the two labels are displayed through staining label by different detection systems and utilizing the differences of target cells stained by each label, information quantity and differential staining on the same radiograph are improved, readability and accuracy of radiograph reading are increased, so that the double-stained kit has important significance to identification of benign or malignant hepatocellular tumor and differentiation degrees of tumor.