Primers for triple PCR of three types of sheep pathogenic mycoplasmas and detection method

Abstract

The invention provides primers for triple PCR of three types of sheep pathogenic mycoplasmas and a detection method. The three pairs of special primers MO, MCC and MCCP are synthesized respectively according to the designs of sheep mycoplasma pneumoniae, a goat mycoplasma mycoide subspecies and a goat mycoplasma pneumonia subspecies, and a triple PCR optimum reaction system and reaction conditions for the three types of sheep pathogenic mycoplasmas are provided. The simultaneous pathogenic detection of the sheep mycoplasma pneumoniae, the goat mycoplasma mycoide subspecies and the goat mycoplasma pneumonia subspecies can be rapidly carried out without cloning, sequencing and sequence comparison, and the detection method has the advantages of being rapid, accurate, strong in specificity, good in repeatability and the like, suitable for rapid detection of the sheep mycoplasma pneumoniae, the goat mycoplasma mycoide subspecies and the goat mycoplasma pneumonia subspecies and large-scale epidemiological investigation and has great economic and social benefits.

Description

technical field [0001] The invention relates to special primers for triple PCR of Mycoplasma ovis pneumoniae, Mycoplasma mycoplasma capricosum and Mycoplasma capricoides subspecies goat pneumonia and a triple PCR detection method, belonging to the field of preventive veterinary medicine. Background technique [0002] In recent years, mycoplasma pneumonia in sheep has been prevalent in Xinjiang, Liaoning, Hebei, Sichuan, Chongqing, Guangxi, Guizhou, Hunan, Fujian and other provinces in China. At the same time, goat infectious pleuropneumonia occurred in my country. The pathogen is Mycoplasma capricosum subsp. goat pneumonia, and mixed infection of more than two kinds of mycoplasma often occurs in the same flock. The clinical symptoms and pathological changes of these two diseases are similar. Difficult to distinguish on the surface, which brings great difficulties to the diagnosis of the disease. These two diseases are the main epidemic diseases of the current sheep raising i...

AI Technical Summary

Problems solved by technology

The current method for diagnosing goat infectious pleuropneumonia and sheep mycoplasma pneumonia is mainly the isolation and identification of pathogens, but the cost of in vitro culture of mycoplasma is high, the culture conditions are harsh, and the growth is slow, especially Mccp is difficult to culture, and it usually takes 2 days to confirm the diagnosis of clinical cases. -3 weeks is obviously unable to meet the needs of rapid diagnosis, so it is urgent to establish a diagnostic method that can distinguish Mycoplasma ovis pneumoniae, Mycoplasma capriformis subsp.

PCR is an important technology for rapid diagnosis of epidemic diseases and epidemiological research. Some foreign scholars have used this technology to diagnose and epidemiological research on goat infectious pleuropneumonia. In China, Qu Yonggang et al., Li Yuan et al., Chu Yuefeng et al. The PCR detection method for Mycoplasma pneumoniae of ovis and the double PCR method for detection of Mycoplasma ovis pneumoniae and Mycoplasma mycoplasma goat subspecies have been developed, but there is no report on the rapid detection of these three kinds of mycoplasma simultaneously at home and abroad.

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