308 results about "Subspecies" patented technology

The invention relates to Agrobacterium mediated transformation of moulds comprising species of the fungal sub-divisions Ascomycotina, Basidiomycotina, Deuteromycotina, Mastigomycotina, and Zygomycotina.Examples demonstrate the transformation of Aspergillus awamori (both protoplasts and conidia), Aspergillus nidulans, Aspergillus niger, Colletotrichum gloeosporioides, Fusarium solani pisi, Neurospora crassa, Trichoderma reesei, Pleurotus ostreatus and Agaricus bisporus (all conidia), and Fusarium graminearum (both conidia and rehydrated freeze dried ATCC material).Especially for Aspergillus awamori the transformation frequency is much higher than with conventional mould transformation techniques.It has further been found that not only one expressable gene can be introduced into these moulds, but even multiple copies of such gene, which, moreover, can be targeted e.g. in the chromosomal pyrG locus, as exemplified for A. awamori. These multiple copies can be of a gene encoding a desired, homologous or heterologous, protein.

Materials and methods for identifying unique sites in bacterial 16S and 23S rDNA are provided, as well as specific unique sequences of 16S rDNA in select bacteria. The distinguishing moieties will enable rapid differentiation between families, genera, groups, species, strains, subspecies, and isolates of microorganisms. Such differentiation can be performed by using rapid screening kits in combination with in silico analysis for diagnostic, prognastic, epidemiologic, phylogenetic, and other purposes.

The invention provides a flexible job shop scheduling method based on multi-species coevolution, which belongs to the field of shop scheduling, and mainly overcomes the disadvantage that a flexible job shop scheduling method based on a genetic algorithm can not be exerted fully. The method comprises the main steps of: 1. setting parameters; 2. initializing species according to a set coding method; 3. calculating the fitness value of each chromosome in each species according to a set method, and recording the optimum fitness value and the constitute chromosomes thereof; 4. carrying out multi-species coevolution: carrying out evolution operations, i.e. chiasmata and variation, to chromosomes in each subspecies, and evaluating new chromosomes; and 5. judging whether a method termination criteria is achieved or not: if so, terminating the method and outputting the optimum fitness value and the constitute chromosomes thereof; and otherwise, jumping to the step 4. The invention can be used to obtain a high-quality scheduling scheme suitable for practical production of shops, can shorten production time, and can be used for scheduling management and optimization of the production process of shops.

A dual-stage method is provided for identifying a microbe by, for example, its species or its subspecies. The method includes measuring a mass spectrum of the microbe using a mass spectrometer, calculating indicators for similarities between reference mass spectra in a library and the measured mass spectrum, selecting a group of reference mass spectra similar to the measured mass spectrum, determining a distinguishing weight for each mass signal of the reference mass spectra in the group, where the distinguishing weights emphasize differences between the reference mass spectra in the group, and calculating indicators for similarities between the reference mass spectra in the group and the measured mass spectrum as a function of the distinguishing weights.

The present invention relates to the technical field of plant gene engineering and concretely relates to the separation clone of paddy wide compatibility gene S5, a function validation and an application thereof for improving the paddy. The segment of the gene contains paddy wide compatibility gene coding aspartic proteinase. Subspecific Indica-Japonica paddy has strong hybrid advantage than varietal paddy, but the sterility of the subspecific Indica-Japonica hybrid restricts using the hybrid advantage thereof. The wide compatibility gene cloned by the present invention can conquer the sterility of the subspecific Indica-Japonica hybrid of the paddy and accordingly uses the strong hybrid advantage of the subspecific Indica-Japonica to further improve the output of the paddy.

The invention is applicable within the food and pharmaceutical industry. More specifically, it relates to a novel strain of the species Bifidobacterium animalis subsp. lactis CECT 8145, the cell components, metabolites and secreted molecules thereof, which, incorporated into food and/or pharmaceutical formulations, can be used in the treatment and/or prevention of excess weight and obesity and related diseases such as metabolic syndrome, hypertension, glycemia, inflammation, type 2 diabetes, cardiovascular diseases, hypercholesterolemia, hormonal alterations, infertility, etc.

The invention relates to a bacillus subtilis subso natto for producing natto kinase and application thereof. The bacillus subtilis subso natto (bacillus subtilis subso natto LNUB236), CCTCC No.: M 208156. The bacillus subtilis subso natto is subject to activation of culture, seed liquid and fermentation liquor are prepared, and the natto kinase is extracted. The invention relates to application of the natto kinase to preparing a health product for preventing and treating cerebral thrombosis. Constituents of the health product are proportioned by weight: lyophilized natto powder : natto kinase : natto kinase : evening primrose oil=1:2.0-3.0:1.0-1.5. The bacillus subtilis subso natto LNUB236 has high natto kinase yield, the highest enzyme activity is 431.455IU/ml in the event of production in a 30l fermenter, and the prepared natto kinase-containing health product has the advantages of high enzyme activity, obvious effect and no side effect.

The use of free, extractable lipids found in bacteria for identification of bacterial species and subspecies is described. Bacteria have been found to differ sufficiently in their extracted lipid compositions to effect identification using thin layer chromatographic techniques. Burkholderia pseudomallei, Burkholderia thailandensis, and Burkholderia mallei have been distinguished in this manner. Lipopeptides specific to Mycobacterium avium subspecies paratuberculosis, but not to the closely related bacterium Mycobacterium avium subspecies avium have also been used as a basis for bacterial subspecies identification using mass spectrometry and seroreactivity. Mass spectrometric analysis of total bacterial lipids of Burkholderia pseudomallei, Burkholderia thailandensis, and Burkholderia mallei, and mass spectrometric analysis of total bacterial lipids for Mycobacterium avium subspecies paratuberculosis and Mycobacterium avium subspecies avium, without further lipid separation, has shown that species and subspecies of bacteria may be identified using such analysis.

The invention relates to a preparation method of (Lactobacillus planetarium subsp.plantarum)Zhang-LL and its listeria monocytogene-resistant bacteriocin. The preparation method is suitable for preservation and fresh-keeping of meat and meat products, milk and milk products, fruits and vegetable, and instant foods. The (Lactobacillus planetarium subsp.plantarum)Zhang-LL (CGMCC No.6936) is selected from bacon on the Fujian farmer's market. A bacteriocin production broth is obtained by fermentation of a Zhang-LL strain, and the Zhang-LL strain bacteriocin is extracted and purified by a pH-dependent adsorption-desorption method, a cation exchange chromatography and a reversed phase high-performance liquid chromatography so that titer is improved by 32 times and purity is improved by 36.65 times. The bacteriocin can inhibit a plurality of food-borne pathogenic bacteria such as listeria monocytogenes, has high bacteriostatic activity, good heat, acid and base stability, can be degraded by human protease and is a natural and safe biological preservative. The preparation method has the advantages of simple processes, stability, high efficiency, source convenience, low cost and industrial production feasibility.

The invention discloses high-protein brown yoghourt and a preparing method thereof.The preparing method includes the steps that 1, processed milk and reducing sugar mixed liquor is homogenized, sterilized, browned and cooled to obtain brown milk; 2, the brown milk is inoculated with starter culture and then fermented to obtain brown yogurt, wherein strains of the starter culture include lactobacillus delbrueckii bulgaricus subspecies, streptococcus thermophilus and lactococcus lactis diacetyl subspecies; 3, the brown yogurt is cooled, passes through a smooth filter, and then is refrigerated and after-cured.The protein content in the high-protein brown yoghourt is 3.63-6.07%, fat content is 4.02-6.75%, solid content is 17.2-30.9%, the amount of active lactobacillus is larger than 5.78*10<8>-3.69*10<9> cfu/mL, and the acidity is 80-100; the yoghourt is light brown, free of food additives and fine and smooth in mouthfeel, has special flavor of a Maillard reaction, can contain dietary fibers and has high nutrition value.

The invention relates to an antibiotic streptomyces, fermentation product of which can inhibit broad spectrum fungus and protozoan, and field experiment can control crop fungus plant disease and root-knot nematode. The antibiotic streptomyces has good effect, no pollution nuisance and no residue. The antifungal agent has extremely low hazardness for the people and good performance and development prospect for medical and agricultural use. The invention is named as SIM001 antibiotic streptomyces which is Streptomyces antibioticus subspecies xi an, and is preserved as patent in the depositary institution appointed by patent office of state intellectual property office; the preservation data is April 14th, 2005, the name of the depositary institution is CGMCC, and the number of preservation is CGMCC No.1349. The whole cell hydrolysate of the antibiotic streptomyces contains L, L-DAP (Diaminopimelic acid) and no characteristic glucide; the cytoderm belongs to I type and glucide type C; one of the main antibacterial materials is polyene macrocyclic ketolide which belongs to the broad spectrum antifungal substance.

The invention discloses a composite strain starter and the preparation method, belonging to the microorganism fermentation field, which is mainly prepared with equivalent lactobacillus delbrueckii Bulgaria subspecies, lactococcus lactis, Leuconostoc mesenteroide, lactobacillus acidophilus, lactobacillus casei, kluyveromyces and brettanomyces. The preparation method is as follow: the strains are optimized and cultivated, and then are respectively fermented, centrifuge-treated, concentrated, added with cell protective agent, and freezed in vacuum. The invention is compounded with various functional strains; the acidophilus milk made by the omposite strain starter has the advantages of original flavor, which has the function indexes close to kefir particle fermentation acidophilus milk, and can be excellent direct distribution kefir starter; the fermented acidophilus milk has strong inhibition effect to pathogenic microorganism, and has good therapeutic effect to gastrointestinal diseases, metabolic disease, hypertension and heart disease.

The invention relates to a functional lactobacillus plantarum plant subspecies having a holesterol lowering function and a preparation method of a compound bacterial powder of the plant subspecies, and belongs to the application fields of functional food and microecologics. According to the preparation method, the lactobacillus plantarum plant subspecies Zhang-LL CGMCC No. 6936 is used for preparing Zhang-LL strain freeze-dried powder by virtue of culture activation, fermentation, bacterial sludge collection and freeze-drying, and the viable count of the freeze-dried powder is 3.50*10<11> CFU/g. Bacillus natto BNZ3 CGMCC No. 9146 is used for preparing bacillus natto power by virtue of culture activation, enlarged cultivation, fermentation and drying, and the activity of nattokinase in the bacillus natto power is 2000IU/g; monacus purpureus Zhang-MP CGMCC No. 9221 is used for preparing monacus purpureus powder by virtue of culture activation, enlarged cultivation, solid-state fermentation, drying and grinding; as being tested, the content of the active substance Monacolin K in the monacus purpureus powder is 4.98mg/g. The prepared lactobacillus plantarum plant subspecies powder is thoroughly mixed with the bacillus natto power and the monacus purpureus powder in the ratio of 1:1:1(w/w), thereby obtaining compound bacterial powder having the serum cholesterol lowering effect.

The invention discloses a construction method and an application of a corynebacterium gene continuous knockout system, belonging to the technical field of genetic engineering. The corynebacterium gene continuous knockout system comprises a template plasmid and a temperature-sensitive expression plasmid, wherein the template plasmid provides a kan fragment (also known as a kan box) with loxp or variant loxL/R sites at two ends; and the temperature-sensitive expression plasmid carries a Cm resistance gene cat and a temperature-sensitive C. glutamicum replicon, expresses a Cre recombinant enzyme and is used for removing a Km resistance gene kan. The corynebacterium continuous knockout system disclosed by the invention can be generally used for continuous gene transformation of corynebacterium and has the advantages of simplicity and convenience in operation and high efficiency. By utilizing the knockout system, gene (continuous) knockout of three major typical subspecies genomes of the corynebacterium can be successfully completed, and the system is proved to be applicable to research and production of corynebacterium metabolites.

The present invention provides an isolated lactic acid bacterium, Lactobacillus plantarum subsp. plantarum PS128 deposited under DSMZ Accession No. DSM 28632. The present invention further provides a composition that comprises Lactobacillus plantarum subsp, plantarum PS128 and a carrier. Moreover, the present invention provides a method for prophylaxis or treatment of a stress-induced disorder in a subject that comprises a step of administering an effective amount of Lactobacillus plantarum subsp. plantarum PS128 to the subject. The present invention also provides a method for prophylaxis or treatment of a functional gastrointestinal disorder in a subject that comprises a step of administering an effective amount of Lactobacillus plantarum subsp. plantarum PS128 to the subject.

The invention provides novel compositions and methods for detecting unlabeled nucleic acid targets using labeled polynucleotide probes and partially complementary antiprobes. The interaction of probes, antiprobes and targets result in signaling changes that indicate target frequency. This novel detection mechanism is called a DNA detection switch, and it enable end-point detection, microarray detection and real-time PCR detection of a variety of nucleic acid targets including microbial species and subspecies, drug resistant mutants, and pathogenic strains.

the invention explains a gamma - amino butyric acid's production method, which uses Streptococcus thermophilus as the bacterial acting on glutaminic acid , glutaminic acid salt , material containing glutaminic acid or glutaminic acid salt to make alpha - he carboxyl of glutaminic acid take decarboxylation reaction , thus turn into gamma - amino butyric acid. This method is a biological synthetic method, does not need high-temperature high pressure reaction condition and harmful raw materials, the uniqueness is high, accessory substances is little and safety is high.

The invention discloses novel application of biopesticide tetramycin generated by Streptomyces ahygroscopicus subsp.Wuzhouensis n.subsp in preventing and controlling Magnaporthe grisea. The tetramycin is compound agricultural antibiotic, comprises tetramycin A1 and A2, albonoursin and flagecidin, and has a rate of preventing and controlling the apple canker of over 95 percent. Indoor and field experiments prove that tetramycin diluent (the ratio of the tetramycin to water is 1: 999) has better effect of preventing and controlling the Magnaporthe grisea than 4 percent kasugamicin waterable powder, 75 percent tricyclazole waterable powder and 25 percent prochloraz missible oil, and has administration lower than positive control remarkably. The tetramycin used can reduce agricultural production cost, improves prevention effect, can be alternately used with other biopesticides, reduces the generation of resistance, has good effect of preventing and controlling the Magnaporthe grisea, and no problems such as environmental pollution, pesticide residue and the like, belongs to the environment-friendly biopesticide, and has good development and application prospects.

The invention provides primers for triple PCR of three types of sheep pathogenic mycoplasmas and a detection method. The three pairs of special primers MO, MCC and MCCP are synthesized respectively according to the designs of sheep mycoplasma pneumoniae, a goat mycoplasma mycoide subspecies and a goat mycoplasma pneumonia subspecies, and a triple PCR optimum reaction system and reaction conditions for the three types of sheep pathogenic mycoplasmas are provided. The simultaneous pathogenic detection of the sheep mycoplasma pneumoniae, the goat mycoplasma mycoide subspecies and the goat mycoplasma pneumonia subspecies can be rapidly carried out without cloning, sequencing and sequence comparison, and the detection method has the advantages of being rapid, accurate, strong in specificity, good in repeatability and the like, suitable for rapid detection of the sheep mycoplasma pneumoniae, the goat mycoplasma mycoide subspecies and the goat mycoplasma pneumonia subspecies and large-scale epidemiological investigation and has great economic and social benefits.

The invention discloses Bacillus amyloliquefaciens subsp. plantarum. The classified nomenclature of the Bacillus amyloliquefaciens subsp. plantarum is Bacillus amyloliquefaciens subsp. plantarum B203; the Bacillus amyloliquefaciens subsp. plantarum was collected in China General Microbiological Culture Collection Center on May 25, 2012; and the collection number is CGMCC No. 6156. The Bacillus amyloliquefaciens subsp. plantarum is mainly used for biological control of strawberry anthracnose. The Bacillus amyloliquefaciens subsp. plantarum B203 has a remarkable inhibitory effect on Colletotrichum fragaliae Brooks, strawberry Botrytis cinerea and various pathogenic fungi of poplar canker, and the antagonistic bacterium also has a good control effect on various other plant diseases; the propagation speed is high; the Bacillus amyloliquefaciens subsp. plantarum can be manually cultured, is easy to operate, and can be conveniently produced and applied; the Bacillus amyloliquefaciens subsp. plantarum has the advantages of high stress resistance and the like, and has an important significance for controlling the strawberry anthracnose caused by fungi; and culture conditions are simple, and the Bacillus amyloliquefaciens subsp. plantarum is easy to preserve, can be industrially produced easily, and has a good development and application prospect.

The invention relates to a method for discriminating microorganism by utilizing Fourier infrared spectrum, comprising the following steps of: a) culturing control microorganism; b) collecting infraredspectrum of the control microorganism; c) establishing a discriminating model of the microorganism in one or a plurality of spectral sections within the interval between 3000-2800cm<-1> and 1800-700c m<-1>; d) culturing the microorganism to be detected under the same condition with the step a); e) collecting the infrared spectrum of the microorganism to be detected under the same condition with the step b); and f) substituting the infrared spectrum obtained in the step e) into the discriminating model of the microorganism and determining the attribution of the microorganism to be detected. Thediscriminating model of the microorganism can realize fast discrimination and does not need to carry out pre-selection to the classification of the microorganism to be detected by using other taxonom ic methods; the method has strong specificity, can carry out classification according to the units of families, classes, species and the like, and even can discriminate to the level of subspecies and serological types; and once the discriminating model is established, the cost for discriminating microorganism is extremely low.

A novel Lactobacillus paracasei subsp. paracasei SG96, a bacteriostatic composition containing the same and use thereof, comprise a novel Lactobacillus paracasei subsp. paracasei SG96 strain, wherein said strain had been deposited in China General Microbiological Culture Collection Center, CGMCC, accession number: CGMCC 2697, characterized in that said strain can inhibit the growth of pathogenic bacteria such as Escherichia coli, Salmonella typhimurium and the like, and even has an effect of killing bacteria; and a bacteriostatic composition containing said Lactobacillus paracasei subsp. paracasei SG96 strain, characterized in that said composition can be used as an additive in animal drinking water, as an additive in animal feed, as an medical composition for animals and humans, as an additive in food products as an additive in beverages, and as a food product, beverage, and health food products, and the like.

The invention discloses a breeding and production method of selenium-enriched yeast with high biomass. Through screening and domestication on a culture medium containing inorganic selenium, a strain of Saccharomyces cerevisiae subspecies Saccharomyces boulardii YBN-2 with high biomass and high content of selenium. Under optimal fermentation conditions, each gram of selenium-enriched yeast enriches as high as 2800-3200 mug / g of organic selenium, has viable count reaching 6.5-8.0*10<10> CFU / g. The selenium-enriched yeast provided by the invention has the advanategs of safety, no toxicity, simple production conditions, low cost and high economic value.

The invention discloses a mannanase and the coding gene, a recombinant vector and a host cell comprising the gene, and expression of the recombinant vector in E.coli and production of the enzyme in yeast cells by using the recombinant vector. The present invention also provides a new wild type subspecies of Bacillus Megaterium, in particular, Bacillus Megaterium FB101.

A method of using a bioactive lysophospholipid (LL) as a biomarker for detecting the presence and recurrence of ovarian cancer. Subspecies of LL, such as lysophosphatidic acid (LPA), lysophosphatidylinositol (LPI), lysophosphatidylcholine (LPC), and lysosphingolipid sphinsosine-1-phosphate (S1P), are used alone or in conjunction to increase the specificity and sensitivity of the assay.