Application of biopesticide tetramycin in preventing and controlling Magnaporthe grisea
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A technology of rice blast and wuningmycin, which is applied in the directions of chemicals, applications, biocides for biological control, etc., can solve the problems of chemical residues, no other, environmental pollution, etc.
Inactive Publication Date: 2010-01-27
辽宁省微生物科学研究院
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Problems solved by technology
At present, the main medicaments for controlling rice blast include: tricyclazole wettable powder, Shi Daoling, Daobunling emulsifiable concentrate, Mieweiwei gel suspension, etc., but there are problems such as drug residues, environmental pollution, and easy to produce resistance.
[0006] As a known compound, Wuningmycin is a composite organism produced by Streptomyces ahygroscopicus subsp.W
Method used
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Embodiment 1
[0122] Embodiment 1: the preparation of Wuningmycin fermented liquid
[0124] (2) Submerged fermentation medium: every 100 ml contains: glucose 2.5g, starch 1.5g, cornmeal 1.0g, soybean cake powder 2.0g, peptone (or corn steep liquor) 0.5g, ammonium sulfate 0.2g, magnesium sulfate 0.03g, 0.02g of dipotassium hydrogen phosphate, 0.7g of calcium carbonate, 0.1g of sodium chloride, 0.2g of vegetable oil, after sterilization, add 0.4% of the medium volume of n-propanol, and the pH is natural.
[0125] (3) Preparation of seed fermentation broth: get Streptomyces anygroscopicus subsp. Wuzhouensis n.subsp strain at an inoculum size of 5-20%, and cultivate it in a seed fermentation medium at 25-3...
Embodiment 2-11
[0127] Example 2-11: Application example of Wuningmycin for controlling rice blast
[0128] Statistical table of raw data of field efficacy test of Wuningmycin on rice blast control (Yiquan Town, Donggang City, Dandong City, Liaoning Province)
[0129]
[0130]
[0131] Statistical table of raw data of field efficacy test of Wuningmycin on controlling rice blast (experimental field of Agricultural Technology Center, Fujin, Heilongjiang)
[0132]
[0133]
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Abstract
The invention discloses novel application of biopesticide tetramycin generated by Streptomyces ahygroscopicus subsp.Wuzhouensis n.subsp in preventing and controlling Magnaporthe grisea. The tetramycin is compound agricultural antibiotic, comprises tetramycin A1 and A2, albonoursin and flagecidin, and has a rate of preventing and controlling the apple canker of over 95 percent. Indoor and field experiments prove that tetramycin diluent (the ratio of the tetramycin to water is 1: 999) has better effect of preventing and controlling the Magnaporthe grisea than 4 percent kasugamicin waterable powder, 75 percent tricyclazole waterable powder and 25 percent prochloraz missible oil, and has administration lower than positive control remarkably. The tetramycin used can reduce agricultural production cost, improves prevention effect, can be alternately used with other biopesticides, reduces the generation of resistance, has good effect of preventing and controlling the Magnaporthe grisea, and no problems such as environmental pollution, pesticide residue and the like, belongs to the environment-friendly biopesticide, and has good development and application prospects.
Description
technical field [0001] The invention relates to the application of Wuningmycin, a biological pesticide produced by Streptomyces ahygroscopicus subsp. Wuzhouensis n.subsp, in preventing and treating rice blast. Background technique [0002] my country is a large agricultural country, with more than 100 million hectares of grain crops, more than 4.8 million hectares of cotton, more than 14 million hectares of oil crops, more than 18 million hectares of vegetables and fruits, and more than 300 million hectares of annual control area. In order to prevent and control pests and ensure the normal production of agriculture, people have used chemical pesticides to prevent and control pests for a long time. The use of chemical pesticides plays an effective role in ensuring the normal production of crops, but with the long-term and large-scale use of chemical pesticides, it has caused serious damage to the ecological environment, such as soil structure damage, reduced enzyme activity i...
Claims
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