The invention provides a full-automatic and high-
throughput micro-fluidic 
chip system and method for detecting 
pesticide residue, used for 
field detection of 
pesticide residue based on 
enzyme inhibition reaction. The full-automatic and high-
throughput micro-fluidic 
chip system is mainly composed of a portable analytical 
detector and a disposable micro-fluidic 
chip; the 
plastic polymer material of the micro-fluidic chip is manufactured through existing micro-
machining technology; the micro-fluidic chip is composed of a central clamping groove, an extraction chamber, a 
sample chamber, a 
reaction chamber, a detection chamber, a micro-groove, a micro-hole and a 
quality control bar code; reagents (comprising an extracting solution, 
enzyme, a colour developing agent and the like) required for detecting 
pesticide residue are fixed in the micro-fluidic chip; the functions of sampling automatically and quantitatively, distributing a fluid, carrying out biochemical reaction and 
molecular recognition and the like can be realized; a fluid operation and control module, a photoelectric detection module, a data analyzing and 
processing module and an 
information storage and communication module are integrated in the portable analytical 
detector; operation and control of biochemical reaction in the micro-fluidic chip and rapid analysis and detection of to-be-detected indexes and 
pesticide residue can be realized; and thus, the micro-fluidic chip 
system and the method provided by the invention are particularly applied to full-automatic and high-
throughput detecting and screening of 
pesticide residue in the samples, such as fruits, vegetables, soil and water.