1261results about How to "Improve experimental efficiency" patented technology

The invention relates to a full-automatic QuECHERS experimental device, and relates to the technical field of sample pretreatment, the full-automatic QuECHERS experimental device comprises an extraction unit and a pipetting unit, the extraction unit comprises: a first tube rack module comprising a plurality of test tube racks; a carrying mechanical arm; the first cap screwing module comprises a tube cap clamping and lifting assembly acting on a tube cap, a tube body clamping and rotating assembly acting on a tube body and a first sliding rail assembly for conveying a test tube to a next station; the sample adding module comprises a homogeneous proton adding module and a salt pouring module; the second cap screwing module comprises a pipe body clamping and rotating assembly, a pipe cap clamping and lifting assembly and a second sliding rail assembly for realizing communication and conveying of the extraction unit and the pipetting unit; the invention discloses a pipetting mechanical arm and a gun head frame. The device is provided with the extraction unit capable of synchronously performing extraction operation and the pipetting unit capable of performing pipetting operation, so that the extraction operation and the pipetting operation are synchronously performed during pesticide residue inspection, and the experimental efficiency and the applicability of the device are effectively improved.

The invention discloses a slope stability test device and test method, and relates to the technical field of slope test.The slope stability test device comprises a test frame and further comprises a test adjusting device.A shielding frame is slidably installed on the inner wall of the test frame, a displacement sensor is fixedly installed on the front side of the shielding frame, and a slope model is arranged at the bottom of the inner wall of the test frame; a placing plate is fixedly installed on the inner wall of the test frame, the test adjusting device comprises hydraulic equipment, an L-shaped frame, an adjusting rod, a pressing plate, an adjusting gear, a U-shaped rod, a limiting frame and an elastic telescopic rod, and the pressing plate moves to make contact with the slope model and conducts a stability test on the slope model; meanwhile, the displacement sensor works to detect and record the displacement condition of the slope model in real time, external force mistaken touch is prevented through the elastic telescopic rod, it can be ensured that the angle of the pressing plate is kept stable in the test process, and therefore the real stability of the slope model is correctly reflected.

The invention discloses an identifying and grading method for saline-alkaline resistance of crops in a germination period, and relates to the technical field of crop planting. In order to solve the problems that in the prior art, due to different saline-alkali soil conditions in different regions, different crops or different varieties of the same crop have different saline-alkali tolerance capacities, and a unified and standard identification process and a grading standard are lacked. Through the steps of standardized seed treatment, seedbed construction and seed sowing, the uniformity and controllability of experimental conditions are ensured, errors caused by experimental operation differences are reduced, the accuracy and repeatability of experimental results are improved, saline-alkali soil environments of different degrees can be accurately simulated by preparing saline-alkali mixed solutions of different concentrations, and the saline-alkali soil quality is improved. The method provides a reliable basis for evaluating the saline-alkaline tolerance of the crop seeds, compares saline-alkaline stress of different concentrations under the same experimental condition, evaluates the saline-alkaline tolerance grade of the crop seeds, and is suitable for saline-alkaline tolerance evaluation of most crop seeds.

The invention provides an intelligent laboratory man-machine interaction control method and system, and relates to the technical field of control, and the method comprises the steps: collecting experimenter and equipment images through a visual collection device, recognizing equipment operation state parameters based on a deep learning model through a processing server, including a switch state, an indicating lamp state, a display screen state and a fault indication state, and transmitting the state parameters to a server; and comparing the identification result with a preset parameter to generate an evaluation result. And the processing server receives the user voice instruction, extracts the content of the control instruction, and determines the target equipment and the control action. And verifying the legality of the instruction and judging whether an execution condition is met by combining an equipment state evaluation result. And a cooperative control strategy is generated according to the control instruction and the evaluation result, the cooperative control strategy comprises a control time sequence, parameters and a data interaction mode, and the control instruction is sent through the field bus network to realize multi-device cooperative control. According to the invention, intelligent management and control of laboratory equipment are realized, and the experiment efficiency and safety are improved.

The invention belongs to the technical field of bacterial culture, and discloses a high-throughput automatic monoclonal screening and picking workstation, which comprises a protective box for protection and isolation, and a workbench is arranged in the protective box; the plate stack module comprises a rotating plate stack arranged at the bottom end of the workbench, and a plurality of columns of culture dishes are arranged on the rotating plate stack in a lifting manner; the transfer module is arranged on the workbench and used for transferring culture dishes; the pipetting module comprises a pipetting assembly and a material storage assembly, and the pipetting assembly is used for transferring strains in the culture dish into the material storage assembly; the identification module is used for identifying and positioning the strains in the culture dish and then controlling the pipetting assembly to accurately transfer the strains. The device is simple in structure and flexible and convenient to use, high-throughput screening and picking of strains can be fully automatically carried out in a clean closed environment, manual intervention is not needed, the screening efficiency is high, strain pollution caused by manual intervention is avoided, the screening precision is high, the screening cost is reduced, and experiments are greatly facilitated.

The invention provides a stirrer fault monitoring method and system, and belongs to the technical field of fault monitoring, and the method comprises the steps: carrying out the installation of a sensor group for a stirrer according to the equipment type of the stirrer; acquiring first data generated in the operation process of the stirrer based on the installed sensor group, and performing data cleaning on the first data to obtain second data; analyzing all the second data, determining initial fault coefficients of the stirrer based on different sensor types, and comparing the initial fault coefficients with standard fault coefficients under corresponding sensor types; and acquiring a comprehensive fault coefficient of the stirrer based on a comparison result, and performing comparative analysis on the comprehensive fault coefficient and a coefficient-early warning comparison table in combination with the initial fault coefficient so as to perform early warning on the current fault of the stirrer. Manual fault judgment is effectively avoided, real-time fault early warning is effectively achieved, then it is guaranteed that the stirrer is in a normal working state continuously and stably, and the experiment efficiency is effectively improved.

The invention relates to the technical field of optical fiber communication, in particular to an optical fiber optical frequency transmission field optical fiber link noise simulation method and system, and the method comprises the steps: dividing laser generated by a laser into reference light and simulation light; modulating the analog light, performing field optical fiber transmission, and performing optical frequency processing on the analog light and the reference light to obtain a radio frequency signal; performing phase processing on the radio frequency signal to obtain continuous phase information and frequency conversion data; the method comprises the following steps: constructing an optical fiber optical frequency transmission field optical fiber noise simulation model, converting phase information and frequency conversion data into voltage data by using the noise simulation model, setting a modulation depth parameter according to the voltage data, performing modulation according to the modulation depth parameter, and adjusting the length of a laboratory winding optical fiber according to a modulation result to realize noise simulation. According to the method, different types and different magnitudes of field optical fiber link noise can be simulated.

The invention belongs to the technical field of molecular identification of aquatic organisms, and particularly relates to a high-sensitivity large yellow croaker germplasm identification primer combination as well as application and an identification method thereof. The primer composition is a first pair of primers LC-1, and the sequences of the first pair of primers LC-1 are as follows: an upstream primer LC-1F: 5 '-AATTCCACACGACTAATC-3'; the downstream primer LC-1R is 5 '-CTCAGCCGCCGTGCAATC-3', and the downstream primer LC-1R is 5 '- The sequences of the second pair of primers LC-2 are as follows: an upstream primer LC-2F: 5 '-AGGCATTAGGCACAAGG-3', a downstream primer LC-2F: 5 '-AGGCATTAGGCACAAGG-3', a downstream And the downstream primer LC-2R is 5 '-AAACCCCTAGCCTCGAAG-3'. The invention also discloses a kit for detecting the disease resistance of the traditional Chinese medicine. The two large yellow croaker germplasm identification primers provided by the invention have relatively high sensitivity and specificity, can be stably amplified in a DNA sample with extremely low concentration, and overcome the limitation that accurate identification cannot be realized due to low DNA concentration in a traditional method.

The invention discloses a rock core clamping device for rock core monitoring, and relates to the technical field of rock core monitoring. The device comprises a rotary clamping disc, a clamping assembly, an injection detection pool and an adjustment limiting system. The flow meter and the weighing assembly are combined to realize accurate measurement of the pore volume of the rock core, the sealing washer and the counterweight pressure block are adopted to improve the sealing performance, the centrifugal force is utilized to enhance the water permeation efficiency, and adaptive adjustment is realized through dynamic adjustment. The device is convenient to operate, accurate in measurement and good in sealing performance, solves the problems of insufficient sealing performance, low detection precision, complex operation and the like in the prior art, and is widely applied to accurate monitoring of the pore volume of the rock core and related experimental research.

The invention provides an observation system for bubble motion characteristics. The observation system comprises an experiment water tank; the gas power generation device is used for pumping gas under different power conditions into the experimental water tank to form bubbles, and the gas power generation device is provided with a gas incidence section extending below the liquid level in the experimental water tank; the observation device comprises a camera platform, a continuous laser and a light uniformizing plate, the camera platform comprises a plurality of side high-speed cameras arranged in an array mode and an upper high-speed camera located above a gas incidence section, and the gas incidence section should be located in an observation area of the camera platform; and the data acquisition and control unit is used for controlling the observation device and pre-processing and analyzing the obtained original data to obtain bubble motion characteristics under different experiment conditions, interaction between bubbles caused by bubble motion and influence of the bubble motion on the flow field. According to the invention, the whole process of bubble generation, development, maturation and formation of a boil-vortex structure on the water surface can be observed.

The invention discloses an animal fixator, which comprises a fixator main body, an animal fixing cavity is defined in the fixator main body, and the fixator main body is provided with a positioning slot communicated with the animal fixing cavity; the positioning inserting plate is movably inserted into the positioning inserting groove, the positioning inserting plate is suitable for extending into the animal fixing cavity, the animal fixing cavity comprises a head fixing area and a body fixing area which are located on the two sides of the positioning inserting plate, and the positioning inserting plate is provided with a neck positioning notch; the pushing piece is movably installed in the animal fixing cavity, and the pushing piece is suitable for moving in the direction close to or away from the positioning inserting groove; and the cover plate is movably mounted at the top of the fixator main body. According to the animal fixing device, an animal can be fixed in the animal fixing cavity, the head cannot rotate, the animal does not need to be anesthetized, operation is easy, consumed time is short, the experiment efficiency is improved, fixing is firm, and the risks of biting and the like possibly caused by manual grabbing are avoided.

The invention discloses a temperature-controllable preparation method for tissue temperature dissociation into single cells and application, the temperature-controllable preparation method for tissue temperature dissociation into single cells comprises the following steps: S1, providing a tissue sample, and pre-treating the tissue sample to obtain a sample block; s2, mixing the sample blocks with a compound digestive enzyme, and filtering to obtain a single-cell suspension after digestion treatment is completed; wherein the compound digestive enzyme is prepared from collagenase, hyaluronidase and papain; and selectively, S3, carrying out red removing treatment. The invention not only provides an improved preparation method of the single-cell suspension, but also has higher efficiency on the premise of ensuring the yield and activity of single cells, and provides a new strategy and tool for cancer research and treatment by particularly enriching CSCs.

The invention provides a position correction method for rapid acquisition of a transmission electron microscope, which is used for acquiring images of the transmission electron microscope at multiple angles. Through mathematical fitting of the relation between the image position (x, y) and the sample rod tilting angle (angle) and the relation between the focusing value (focus) and the sample rod tilting angle (angle), the position error and the focusing change caused by tilting are effectively corrected, so that the alignment precision and the imaging quality of the image are improved; according to the method, correction efficiency and automation can be greatly improved by substituting accurate numerical values into a fitting function, and the method has remarkable technical progress and application value in the aspect of improving multi-angle imaging and three-dimensional reconstruction of the transmission electron microscope.

The invention belongs to the field of image spectrum detection, and particularly relates to a high-precision detection device and method for a liquid meniscus contour in a capillary tube. Comprising a telecentric lens, an industrial camera, a displacement platform, a light source, a capillary tube and a culture dish, the industrial camera, the capillary tube and the culture dish are installed on different displacement platforms respectively, the light source is provided with a rotary telescopic rod and can move in all directions, when the liquid meniscus contour in the capillary tube is shot, the light source, the meniscus of the capillary tube and the industrial camera are adjusted to be aligned to the same horizontal plane, and on the basis of the telecentric imaging principle, according to the light refraction principle, the liquid meniscus contour in the capillary tube is obtained. And deriving a distortion correction function of the meniscus contour in the capillary tube. According to the invention, the industrial camera and the telecentric lens are matched for use, the problem of shape distortion caused by object distance change is avoided, and the difficulty of image distortion correction is reduced. The meniscus contour distortion correction function derived based on the telecentric imaging principle can accurately calculate the actual value of the meniscus contour and restore the actual meniscus contour.

The invention relates to an experimental device and method for studying the partial discharge aging mechanism of an insulating material, and belongs to the technical field of insulating materials. Comprising a multifunctional closed cavity, a high-voltage circuit, a detection loop and a monitoring sensor, and can accurately control the environment atmosphere, the discharge gap and the discharge voltage, thereby simulating the actual complex operation condition of an insulating material under the high-voltage condition, and monitoring the light intensity, the discharge amount and the discharge energy in the discharge process. The device can obtain solid residues of an insulating material under different discharge conditions and collect gas and liquid products. The analysis method not only considers the partial discharge instantaneous heat effect, but also covers the comprehensive effects of active products, ultraviolet radiation and the like in the partial discharge process, so that the aging mechanism of the insulating material under the partial discharge effect is comprehensively analyzed.

The invention relates to the technical field of data processing, and discloses an experimental process data recording method and system based on biological recognition and a storage medium. The method comprises the following steps: performing voiceprint recognition and natural language processing on a real-time voice instruction of a detection operator to obtain an experiment operation record; performing real-time acquisition and anomaly detection processing on the data of the detection instrument to obtain safety index data and potential risk alarm; step optimization analysis is carried out on the experiment operation records, the safety index data and the potential risk alarms, and detection step optimization suggestions are obtained; and performing time serialization integration processing on the experiment operation record, the safety index data, the potential risk alarm and the detection step optimization suggestion to obtain a traceable experiment report and a detection safety level evaluation result. According to the invention, the efficiency and accuracy of experimental process data recording based on biological recognition are improved.

The invention discloses a GIS equipment electromechanical state and typical defect simulation test platform and a GIS equipment electromechanical state and typical defect simulation test method, relates to the field of electrical equipment electromechanical state and typical defect simulation tests, and solves the problem of simulation and research of electromechanical states and defect characteristics of gas insulated switchgear (GIS) under different operation conditions. Wherein the test platform comprises an AC voltage source, a GIS scaling model, a vibration table and an excitation power amplifier, defects are preset in the GIS scaling model, the GIS scaling model is provided with a transparent observation window used for observing the internal condition of the model, and the GIS scaling model is fixedly installed on the vibration table; an alternating-current voltage source is loaded on a GIS scaling model bus to simulate distribution of an electric field in a GIS, and vibration is applied to the GIS scaling model through a vibration table and an excitation power amplifier to simulate GIS vibration caused by current.

The invention relates to a digital core resistivity numerical simulation and saturation model construction method under deep overpressure and high temperature conditions, and relates to the technical field of oil-gas exploration, and the method comprises the steps: constructing a digital core under the overpressure condition, calculating the clay conductivity and formation water conductivity under the high temperature condition, and obtaining an overpressure and high temperature equivalent digital core model; based on an overpressure and high-temperature equivalent digital core model, through an electrical numerical simulation algorithm, the resistivity and the stratum factor F of water-saturated cores with different porosities phi are calculated, and an F-phi cross plot is constructed; and calculating digital core resistivity and resistivity increase coefficient RI of different water saturation Sw by using a numerical simulation algorithm, and constructing an RI-Sw cross plot. According to the method, a saturation model under the deep actual temperature and pressure conditions can be constructed through equivalence of pressure and temperature by utilizing a digital rock core numerical simulation technology.

The invention provides a standardized experiment method and device for a rock mechanics experiment, and the method comprises the steps: collecting a plurality of conventional rock mechanics experiment schemes, determining a plurality of experiment characteristics of each experiment, and splitting links of each conventional rock mechanics experiment according to the plurality of experiment characteristics; according to the standard format corresponding to each experiment feature, storing each experiment feature of each experiment into a corresponding storage file to generate a standardized description of the rock mechanics experiment; performing matching analysis on each experiment characteristic of the target sample and the standardized description, and determining a target experiment type corresponding to the target sample; and extracting a corresponding experiment scheme from the standardized description according to the target experiment type, and performing an automatic experiment on the target sample to obtain the mechanical index of the target sample. The method is based on standardized experimental scheme description, can carry out batch and automatic rock mechanics experiments, improves the experiment efficiency, and reduces the experiment cost.

The invention discloses an anti-impact quartz flexible accelerometer swing beam and a design method, relates to the technical field of accelerometer structure design, and solves the problem that the swing beam is easy to break when the existing swing assembly is sensitive to strong impact and high vibration in the directions of a swing shaft and an output shaft. The invention provides a shock-resistant quartz flexible accelerometer pendulum beam which is assembled in a pendulum assembly of a quartz flexible accelerometer comprising a quartz pendulum piece and a coil assembly, the quartz pendulum piece comprises a pendulum beam, a pendulum tongue and a pendulum piece outer ring, and the coil assembly comprises a coil framework and a coil winding. The swing beam comprises a reinforcing boss arranged on the surface of the swing beam.

The invention relates to the technical field of embryo culture platforms, in particular to a microfluidic-AI monitoring-metabolism regulation embryo culture platform and a preparation method thereof.The microfluidic-AI monitoring-metabolism regulation embryo culture platform comprises a microfluidic culture system used for achieving embryo culture, metabolite removal and culture medium replacement; the artificial intelligence monitoring system is used for remotely monitoring the embryonic development state in real time and predicting the embryonic development condition; the metabolism regulation and control system is used for feeding back and optimizing culture conditions online according to an artificial intelligence monitoring result and a metabolite analysis result; the intelligent control module is electrically connected with the micro-fluidic culture system, the artificial intelligence monitoring system and the metabolism regulation and control system, and the micro-fluidic culture system can accurately control the culture environment on the micrometer scale, including temperature, pH value and gas composition parameters. The dynamic fluid environment is closer to the in-vivo physiological environment of the embryo. The system can simulate periodic nutrient supply and metabolic waste removal in the uterus, and provides optimal growth conditions for embryos.

The invention discloses a glutamate decarboxylase multi-site combined mutant and application thereof, and relates to the technical field of enzyme engineering and gene engineering. Amino acid residue sites needing to be mutated are determined on the basis of amino acid sites near key amino acids of a coenzyme 5 '-phosphopyridoxal binding pocket, and experimental verification is performed through a site-directed mutagenesis technology in combination with a high performance liquid chromatography screening method. Compared with a chemical synthesis method, the compound is synthesized by utilizing glutamic acid decarboxylase through biological catalysis, the synthesis condition is mild, the operation is simple, and separation and extraction are easy. According to the method, the screening probability of mutant amino acid sites can be effectively improved, the experiment efficiency and feasibility are improved, and the mutant enzyme of which the enzyme activity is obviously superior to that of the parent enzyme is obtained through screening.

The invention discloses an automatic liquid perfusion system and application of the automatic liquid perfusion system in multi-round space transcriptional group imaging. The automatic liquid perfusion system is formed by sequentially connecting a plurality of multi-channel switching valves connected in series, a peristaltic pump and a multi-channel switching valve. By means of the automatic liquid perfusion system, full-automatic perfusion can be achieved in the multi-round space transcriptome imaging process, perfusion of multiple samples can be achieved through sequential control, and the experiment efficiency is remarkably improved. And the sample does not need to be repeatedly detached from the imaging system in the whole process, so that subsequent data analysis is facilitated, and the imaging accuracy can be improved.

The utility model provides a needle type on-line filtering device which comprises a filter element placing block, a first notch and a second notch which are horizontally and transversely arranged at the upper end of the filter element placing block; the sample loading frame is arranged on the horizontal side surface of the filter element placing block and can be used for placing at least one row of test tubes which are horizontally and transversely arranged with openings facing upwards; the vertical mechanical arm is provided with a lifting seat which is located on the upper portion and can ascend and descend and a guide seat which is located on the lower portion and fixed, the lifting seat is used for fixing the upper end of a sample loading needle, and the guide seat can allow the lower end of the sample loading needle to penetrate through; the filter element is stacked in the first notch of the filter element placing block and can be connected with the sample loading needle in a tight fit manner; and the horizontal longitudinal mechanical arm can drive the filter element placing block and the sample loading frame to horizontally and longitudinally move. Therefore, the functions that the sampling needle pricks the filter element at the first notch of the filter element placing block, filters and samples in the test tube of the sampling frame, and rows the filter element at the second notch of the filter element placing block can be realized.

The invention belongs to the field of artificial intelligence algorithm application-biological sequence recognition, and relates to a method for recognizing phosphorylation modification sites based on a large model technology. According to the method, the latest protein large model technology ESM-2 is applied to the phosphorylation modification site recognition direction, the current forefront technology is fully utilized to recognize the phosphorylation modification site infected by SARS-CoV-2, and the recognition accuracy and reliability are remarkably improved. According to the method, the intermediate output of the protein pre-training large model is innovatively extracted for subsequent research. For different tasks, different level output features may be needed, the research provides a clear example, and the effectiveness of model intermediate layer output is displayed. According to the method, transfer learning related technologies are creatively used, so that the problem that classification prediction cannot be effectively carried out due to the fact that tyrosine (Y) data in SARS-CoV-2 infected phosphorylation modification sites are rare is effectively solved.

The invention relates to a method for preparing a heterostructure magnesium alloy based on low-temperature friction stir additive in the field of metal processing, and designs a high-quality and high-efficiency cooling tool, which mainly comprises the following steps: firstly, cooling alcohol to low temperature by using liquid nitrogen; then the magnesium alloy plate to be machined is fixed to a cooling tool in a lap joint mode, and the tool mainly comprises a device body (1), a cooling liquid inflow hole (2), a cooling liquid outflow hole (3), a workpiece containing groove (4), a liquid storage chamber (5), a pressing plate (6) and fastening screws (7); then low-temperature alcohol is introduced, an advancing path and technological parameters are set, first-layer stirring friction additive manufacturing is conducted, and in the machining process, a stirring head and a workpiece are always kept to be submerged in cooling liquid; then, the surface obtained after material adding is milled to be flat, a layer of plate is overlaid on the other unmachined surface again, and the plate is fixed into the workpiece containing groove; and finally, second-layer friction stir additive manufacturing is conducted on the other face of the plate according to the same parameters and the same path. The method can be suitable for additive manufacturing of low-melting-point metal such as magnesium alloy and aluminum alloy at the same time, the multi-scale gradient heterostructure magnesium alloy can be prepared at the same time, the preparation efficiency is improved, and high-quality and low-cost preparation of heterostructure materials is achieved.

The invention relates to a gyrator and method for plant microgravity effect simulation, the gyrator comprises a main body box and a movable light source, the main body box comprises a plurality of modules spliced in series, the modules are provided with a plurality of clamps, and the clamps are used for placing plant culture containers and driving the plant culture containers to rotate; the movable light source is arranged on the front side of the main body box, the distance between the movable light source and the main body box is adjustable, and the movable light source is used for providing illumination for the plant sample. The device has the beneficial effects that the technical problem that the existing domestic microgravity simulation device cannot perform large-scale measurement and development condition tracking on mature plants is solved, and the experimental materials for the existing simulator are animal cells, plant seedlings, single plant adult seedlings and the like; and large-scale microgravity simulation of the overground part of the mature plant in a unified environment cannot be met. By means of the gyrator, the growth and development reactions of the overground parts of various plants under microgravity can be tested in batches.

The invention discloses a device and method for measuring the mechanical property of a thick oil-water interface by simulating oil reservoir conditions, and relates to the technical field of chemical cold production of thick oil, and the device comprises a thick oil injection module, a water injection module, a high-temperature and high-pressure bin module and a data acquisition and calculation and injection pump control module; the thick oil injection module is connected with the high-temperature and high-pressure bin module, and the thick oil injection module is configured to inject thick oil into the high-temperature and high-pressure bin module; the water injection module is connected with the high-temperature and high-pressure bin module and is configured to inject water into the high-temperature and high-pressure bin module; and the data acquisition calculation and injection pump control module is connected with the high-temperature and high-pressure bin module and the thick oil injection module, and is configured to acquire parameters such as temperature and pressure in a high-temperature and high-pressure cavity, obtain a hanging drop outline image in the high-temperature and high-pressure bin module and control the thick oil injection process in the thick oil injection module. The device solves the problem that most devices at present cannot measure the mechanical properties of the heavy oil-water interface under the real oil reservoir condition.

The invention relates to cloning of a translocation recombinant fragment in gibberellic disease resistant wheat Zhongke 1878, molecular marker development and breeding application. Specifically, the invention relates to a specific molecular marker of the gibberellic disease resistant wheat Zhongke 1878, and the nucleotide sequence of the molecular marker is shown as SEQ ID NO: 4. The invention also relates to application of the molecular marker in breeding and / or identification of wheat resistant to gibberellic disease. In addition, the invention also relates to a recombinant site RS-6DL / 7EL in the gibberellic disease resistant wheat Zhongke 1878, and the nucleotide sequence of the recombinant site RS-6DL / 7EL is as shown in SEQ ID NO: 1. The invention also relates to application of the recombinant site RS-6DL / 7EL in breeding and / or identification of wheat resistant to gibberellic disease. The invention has important significance on wheat scab resistance breeding.

The invention relates to the technical field of chemistry, and particularly discloses a device and method for measuring freezing point decline, and the device comprises a hood shell, a control system and a measuring mechanism; the control system is installed on the right side of the exterior of the hood shell and electrically connected with the hood shell. The measuring mechanism is arranged below the hood shell and comprises a bottom shell, a track platform, a clamping mechanical arm, a test tube rack, a liquid injection part, a solid adding part, cooling bath manufacturing equipment, a measuring part, test tube cleaning and drying equipment, a first pump body, a second pump body and a sensor. According to the device for measuring freezing point drop and the measuring method, the freezing point is measured by adopting full-process automatic operation, the experimental environment and process are more stably controlled, the consistency of experimental operation is ensured, the measuring time of a single sample is shortened, and the overall experimental efficiency is improved.