The invention discloses a drought
simulation culture method adaptive to rice SEUSS
gene function
verification, and belongs to the technical field of
plant gene function
verification. According to the method, by taking a
japonica rice variety Nipponbare as a background and aiming at specific requirements of SEUSS
gene function
verification, accurate analysis of the SEUSS gene
drought resistance regulation and
control function is realized through a technical
route of homogenization pretreatment of experimental materials, pre-culture of a standard
nutrient solution, PEG-6000 and NaCl double-stress drought
simulation, dynamic regulation and control of stress intensity and
phenotype-molecule combined verification after rehydration. The method specifically comprises the steps of seed disinfection and
germination, two-leaf one-core stage
seedling stress treatment, rehydration
survival rate statistics,
root length /
seedling length /
dry weight determination, seed shape analysis,
genotype rechecking,
gene expression quantity detection and the like, and meanwhile, a PCR amplification
system of a high-GC-content gene and a
mutant seed propagation scheme are optimized. The problems that an existing drought
simulation method is poor in pertinence,
stress conditions are difficult to control, and result reliability is low are solved, and a standardized and repeatable experiment
system is constructed.