The present invention discloses an experimental method for monitoring and quantifying the acoustic behavioral responses of fish, comprising: an
experimental system, which includes: a circulating water tank for placing a fish school; a 3D optical sensor for obtaining a set of spatial coordinates of the fish school; a plurality of first
acoustic wave transmitters for simulating an
underwater dynamic acoustic environment; and a plurality of second
acoustic wave transmitters for directionally transmitting acoustic
waves, the
directivity of which is controlled by the set of spatial coordinates of the fish school. The
experimental system of the present invention integrates an acoustic stimulation emission
system, a circulating water temporary culture
system, and a fish behavior
recording system, providing an efficient, accurate, and comprehensive means for studying the acoustic stimulation behavior of fish. It is used to monitor, evaluate, and quantify the three-dimensional behavioral responses of experimental fish schools under
scenario acoustic stimulations of different intensities and types in conditions simulating natural
aquaculture sea conditions, and to characterize their kinematic parameters. When no experiment is being carried out, the experimental fish can also be stably temporarily cultured in the
experimental system to achieve stable and real-time regulation of
water quality.