The invention provides a two-dimensional microcosmic visual thickened oil replacement simulation experiment system and a use method thereof. The two-dimensional microcosmic visual thickened oil replacement simulation experiment system comprises a microcosmic simulation experiment device, a visual data acquisition device, a support device, a multivariate thermal fluid injection device, a confining pressure tracking device and a metering device, wherein the microcosmic simulation experiment device comprises a heating jacket, a clamper and a plate glass microcosmic model; the visual data acquisition device comprises a microscopic shooting device and a computer; the multivariate thermal fluid injection device comprises an ISCO injection pump, an oven and a replacement medium intermediate container, a water intermediate container, a thickened oil intermediate container, a first heat traced pipeline and a bypass pipeline; the confining pressure tracking device comprises an annular pressure tracking pump, an annular pressure tracking pump pressure meter, a vacuum pump and a first vacuum valve. The two-dimensional microcosmic visual thickened oil replacement simulation experiment system give full consideration to characteristics of the thickened oil reservoirs, provides convenience for research on a thickened oil replacement mechanism through microcosmic experiments, has the advantages of low experiment cost, high work efficiency and the like, and can meet with demands on development and research of the thickened oil reservoirs.