The utility model discloses a closed environment experimental device, which relates to the technical field of experimental equipment, including a box and an adjustment component, wherein an exhaust hole is provided at the center of the top of the box, and a fixed box is arranged in the middle of the top surface of the box, and the top of the fixed box is connected with an adjustment component, and the adjustment component includes a knob, a screw sleeve, a screw, an adjustment plate, a slider, a slide bar, a sealing plug and a spring, and the bottom of the knob is connected with a screw sleeve, and the internal thread of the screw sleeve is connected with a screw. The closed environment experimental device is provided with an adjustment component, and when it is necessary to change the pressure relief condition, only the knob needs to be turned to drive the screw sleeve to rotate, and at this time the slider will limit the rotation of the adjustment plate and the screw, so the height of the adjustment plate can be controlled by the screw, and when the height of the adjustment plate is reduced, the spring is compressed, so that a larger pressure can be provided to the sealing plug, and when the adjustment plate is raised, the pressure provided by the spring will be reduced.