2880results about How to "Simple and fast operation" patented technology

The invention belongs to the technical field of cosmetics, and particularly relates to a skin-color-uniformizing whitening composition derived from plant extracts and application of the skin-color-uniformizing whitening composition. The whitening composition provided by the invention is prepared from the following components in parts by weight: 10 to 15 parts of saussurea involucrata extract, 1 to 8 parts of dendrobium officinale extract, 1 to 5 parts of wood apple extract and 0.5 to 5 parts of banana fruit extract. The four plant extracts are synergistically compounded, transdermal absorption of active ingredients can be promoted, the tyrosinase inhibition rate is effectively increased, and the skin glossiness and whiteness are improved, so that the effects of whitening, brightening and uniformizing the skin color are achieved, the whitening composition is mild and non-irritant, the preparation method is simple and convenient to operate, and the whitening composition has large-scale production capacity and industrial feasibility. When the whitening composition is applied to skin care products such as face cream, the whitening and brightening effects of the face cream can be effectively improved, and the whitening composition has wide market application value.

The invention discloses a multi-parameter distortion compensation method for a fractured rock mass seepage similar model test, and relates to the technical field of rock mass engineering physical model tests. The method comprises the following steps of: firstly, determining two constraint conditions that the fluid property is invariable and the gravitational acceleration is invariable, correspondingly selecting a basic physical quantity and extracting a core criterion; then analyzing three types of distortion sources of geometric dimensions, dielectric materials and boundary conditions, and establishing a distortion function according to result parameters of seepage flow velocity, seepage flow and seepage pressure; secondly, parameters difficult to control are selected as distortion items, parameters easy to regulate and control are selected as compensation items, the distortion items are substituted into a distortion function to obtain a distortion ratio, the optimal distortion ratio of the compensation items is solved based on a compensation balance formula, and the parameters are adjusted; and finally verifying the interval range of the actually measured distortion ratio. According to the method, the multi-source distortion coupling influence is accurately quantified, the compensation pertinence and precision are improved, operation is easy and convenient, universality is high, and reliable support is provided for underground engineering seepage research.

The invention belongs to the cross technical field of remote sensing data processing and soil environment monitoring, and discloses a multi-source remote sensing collaborative soil quality index inversion and comprehensive evaluation system and method, and the method comprises the following steps: obtaining different types of remote sensing data in a target area, and constructing a multi-source data set; the acquired multi-source remote sensing data are preprocessed, data noise and system errors are eliminated, and data consistency is ensured; performing multi-source remote sensing data fusion by adopting a spectrum-polarization-texture collaborative fusion strategy in combination with a multi-scale decomposition and weighted fusion model; based on the fusion data, constructing a machine learning inversion model, and inverting soil quality key indexes; and carrying out comprehensive evaluation on the soil quality by adopting an analytic hierarchy process-matter element extension model to realize grading of the soil quality. By adopting the multi-source remote sensing collaborative soil quality index inversion and comprehensive evaluation system and method disclosed by the invention, rapid, accurate, large-area and dynamic monitoring and comprehensive evaluation of soil quality are realized.

The invention belongs to the technical field of fiber reinforced composite material recovery, and particularly relates to a full-process recovery method of a fiber reinforced composite material. The modified fiber is prepared by constructing a dynamic coordination crosslinking interface layer formed by Co < 2 + > and phenolic hydroxyl groups and amino groups in polydopamine on the surface of the fiber. And then, preparing the fiber reinforced composite material by taking the modified fiber and resin as raw materials. A fiber reinforced composite material is placed in a coordination competitor, cobalt-oxygen and cobalt-nitrogen coordination bonds of an interface layer are competitively dissociated and interface bonding is destroyed under the heating condition by utilizing the stronger coordination capacity of the coordination competitor to Co < 2 + >, and then ultrasonic treatment is assisted, so that efficient and nondestructive separation of fibers and a resin matrix is realized. The process method is simple, convenient to operate, green and environmentally friendly, and has important practical significance and industrial application prospects.

The utility model provides an underwater robot for bridge detection, which relates to the technical field of bridge detection, and comprises a robot main body, a floating component for providing floating force is arranged at the upper part of the robot main body; the propeller set comprises two side propellers and two tail propellers, the two side propellers are located on the front face of the robot body, and the two tail propellers are located at the same end of the buoyancy assembly and symmetrically arranged front and back; and the two symmetrical walking mechanisms are arranged on the robot main body through mounting structural members. The beneficial effects of the utility model are that through the reasonable layout of the propeller group and the cooperative work of the propellers at the two sides and the tail part, accurate water surface positioning and movement are realized, and the robot is ensured to smoothly approach and fit a pile foundation from the water surface; the posture of the whole machine does not need to be adjusted when the pile foundation is close to the pile foundation, and the pile foundation can be directly adsorbed and attached through the propellers on the two sides; a tensioning mechanism in the walking mechanism has self-adaptability, and an elastic support is matched with a connecting frame connected in a sliding mode.

This invention discloses a primer and probe set, a kit, and their applications for detecting Staphylococcus aureus L based on real-time fluorescence MIRA technology. The primer and probe set consists of single-stranded DNA sequences 1-3. Based on the aforementioned primer and probe set, this invention establishes and optimizes a real-time fluorescence MIRA detection method for Staphylococcus aureus L. Furthermore, the optimized real-time fluorescence MIRA method is innovatively combined with a simplified nucleic acid extraction method and a portable fluorescence detection device, significantly reducing extraction and time costs while enabling rapid on-site detection of the virus, making it particularly suitable for grassroots units and remote areas. In addition, the real-time fluorescence MIRA detection method for Staphylococcus aureus L established in this invention has advantages such as high specificity, high sensitivity, and simple operation. Moreover, the detection process does not require opening the lid, effectively avoiding aerosol contamination, providing an effective technical means for the differential diagnosis and rapid detection of Staphylococcus aureus L.

The invention relates to the technical field of special polymer functional membranes, and discloses a polybenzimidazole gas separation membrane as well as a preparation method and application thereof, the polybenzimidazole gas separation membrane has an interpenetrating network-shaped pore structure, and the polybenzimidazole gas separation membrane has a nanoscale pore structure. The pore size distribution of the nanoscale pore structure is 0.1-30 nm, and the average pore size of the nanoscale pore structure is 1-80 nm. The polybenzimidazole gas separation membrane has an interpenetrating network-shaped pore structure and a nanoscale pore structure, so that the polybenzimidazole gas separation membrane has relatively high gas selectivity and permeability coefficient and also has relatively good mechanical properties.

The invention belongs to the technical field of copper rod machining, and particularly discloses a copper rod machining device and a machining process thereof.The copper rod machining device comprises a base, two sets of workpiece supporting frames, a lifting frame, two sets of cutting machines and a transverse embracing mechanism, and the two sets of workpiece supporting frames are symmetrically installed on the upper surfaces of the two sides of the base; the two sets of workpiece supporting frames are correspondingly connected with sliding blocks through bidirectional driving pieces correspondingly arranged in the two sets of workpiece supporting frames, assembling rods are fixedly installed on the outer sides of the two sets of sliding blocks correspondingly, and a first guide frame and a second guide frame are arranged between the two assembling rods with the opposite end faces correspondingly. According to the copper rod cutting and polishing integrated machining device, copper rod cutting and polishing integrated machining is achieved, the cutting position is accurately controlled, end face burrs are effectively removed, the equipment investment and the operation cost are reduced, the machining efficiency is improved, and the machining quality is improved. And the machining efficiency and the yield are improved, and the production requirements of small and medium-sized copper material machining enterprises are met.

The invention discloses a steel structural member local corrosion intelligent identification and evaluation method based on deep learning, and the method comprises the steps: obtaining a steel structure surface image through an imaging device, carrying out the feature extraction and corrosion region identification of an input image through a YOLOv12 semantic segmentation model which introduces a CBAM self-attention mechanism, and generating a pixel-level corrosion mask; the actual physical area of the corrosion area is automatically calculated based on an area calculation algorithm of conversion of mask integration and ground sampling distance; the residual bearing performance of the corroded component is evaluated in combination with the corrosion area ratio and the morphological characteristic prediction model; and finally realizing visual display and report output of detection results, area data and performance indexes through a graphical interface. According to the invention, full-process automatic processing of local corrosion of the steel structural member from detection, segmentation, quantitative measurement and calculation to member performance evaluation is realized, and closed-loop intelligent evaluation from visual detection to mechanical evaluation is realized.

This application provides a method for preparing compound I-b or a pharmaceutically acceptable salt thereof, wherein (S)-N-methylamino-1-(2-thiophene)-1-propanol reacts with 4-fluorobenzo[1,3]dioxolane in a system containing sodium hydride and potassium benzoate to obtain compound I-b. This method has short steps, mild reaction conditions, and is simple to operate, making it suitable for industrial scale-up; moreover, the obtained product has high purity and low isomer content.

The invention discloses a method for in-situ passivation of available cadmium in soil, and belongs to the technical field of heavy metal contaminated soil treatment. According to the method, the fulvic acid with the molecular weight being 100 kDa or above is applied to the acid mining area soil with the pH being 2-4.5 according to the adding amount of 3-20 gC / kg, the low-concentration cadmium pollution of the source being 4 mg / kg or below is removed, the concentration of effective-state cadmium is reduced, the cost is saved, the natural substance fulvic acid is used as the remediation agent, operation is easy and convenient, and the method is suitable for remediation of various mining area soil.

The invention relates to the technical field of lasers, and provides a double-gain-medium Q-switched laser and a constant-power pulse width adjusting method.The laser comprises a first pumping source, a second pumping source, an optical fiber beam combiner, a beam shaping system and a laser resonant cavity, the laser resonant cavity comprises a reflecting mirror, a first gain medium, a second gain medium, a polarization selector, a quarter-wave plate, a Q switch and an output mirror which are arranged in sequence; the first gain medium is used for absorbing first pumping light, the second gain medium is used for absorbing second pumping light, and the pumping power of the first pumping source and the pumping power of the second pumping source can be independently controlled. By changing the pumping power of the two pumping sources, real-time adjustment of the pulse width of the laser under the constant output power is achieved, the structure is compact, and control is easy and convenient.

This invention relates to the field of oilfield thermal recovery well technology, and discloses a well completion process device, a column-type ground anchor, and a method for increasing the prestress of the oil layer casing. The well completion process device includes a surface casing (1) located below the surface, an oil layer casing (2), and a casing head body (3). The oil layer casing is connected to the inner surface of the casing head body via a connecting assembly (40). A column-type ground anchor (500) is connected to the bottom of the oil layer casing. The column-type ground anchor includes an anchor claw (502) and a plunger body (530). A liquid outlet (501) is formed on the column-type ground anchor. The anchor claw can extend out of the side wall of the plunger body and insert into the formation. Cement can be discharged from the liquid outlet to seal the annular gap between the oil layer casing and the formation. This invention uses hydraulic oil to insert the anchor claw into the formation, pulling the oil layer casing to a preset load. After the oil layer casing is pulled up, it is clamped to the inner wall of the casing head body via the connecting assembly, thus increasing the prestress.

The invention relates to the technical field of ferrous metallurgy casting, in particular to a drainage sand removing and recycling method and device. Comprising a mechanical arm, a lifting rotation controller, a controller arm, a sand suction cylinder, a sand conveying pipeline, a sand collecting tank and an injection pump. The lifting and rotating controller is fixedly mounted on the mechanical arm; the controller arm is connected with the lifting and rotating controller, and the lifting and rotating of the controller arm are controlled by the lifting and rotating controller; the sand suction cylinder is connected with the controller arm, the sand suction cylinder is of a U-shaped structure, and one end of the sand suction cylinder is a horn mouth used for sucking drainage sand; one end of the sand conveying pipeline is positioned above the tundish cover sprue gate, and the end part of the sand conveying pipeline is bent downwards to be connected with the other end of the sand suction cylinder; the other end of the sand conveying pipeline is connected with the sand collecting tank; the sand collecting tank is connected with an injection pump pipeline, and the injection pump provides suction power. The ladle filler sand, harmful gas in gaps of the ladle filler sand and a long nozzle and a small part of molten steel in contact with the ladle filler sand in a steel ladle can be discharged out of tundish molten steel and recycled.

The utility model discloses a rescue air cushion convenient to carry, including box, air bag pad and movable board, the inside of box is provided with the cavity, and the one side of box is provided with the notched of being linked with the cavity, and the upper and lower both ends of notched inboard all are through the pivot respectively installed with roll axle no.

The application discloses a synthesis method of a large single-crystal sodium-ion battery layered oxide positive electrode material, and comprises the following steps: (1) weighing metal oxides containing transition metal elements, transferring to a device with mixing functions, uniformly mixing, and obtaining a metal oxide mixture; (2) adding acid to the metal oxide mixture, fully mixing, and completing an acid treatment process; (3) adding alkali to the mixture after acid treatment, fully mixing; (4) transferring the mixture obtained in the step (3) to a calcining furnace, high-temperature calcining, and obtaining a layered oxide. The acid treatment process is introduced, the surface of the metal oxide forms a defect structure under the action of the acid, and the defect structure is more beneficial to the formation of strong interaction between each component of the metal oxide and between the metal oxide and sodium-containing alkali, so that a larger single-crystal layered oxide structure is finally formed, and the specific capacity, rate performance and cycle performance are excellent.

This utility model relates to the field of children's stroller technology and provides a children's stroller bassinet locking mechanism, including a front armrest seat, a front frame, a rear frame, an unlocking component, a gear, and a fixing seat. The fixing seat is hinged to the rear frame, front frame, and front armrest seat at the same center. The gear is located between the rear frame and the fixing seat and can slide inside. In this utility model, a reliable mechanical locking is achieved by setting a sliding gear between the rear frame and the fixing seat. The inclined surface fit structure between the unlocking component and the gear makes the unlocking process smooth and stable. Unlocking can be completed by a one-way sliding action, which is easy to operate. The fixing seat, front frame, rear frame, and front armrest seat share the same central hinge point, reducing the number of parts and making the structure more compact. This design not only improves the overall strength but also maintains the stability of the frame posture during locking and unlocking. The mechanism can be hidden inside the frame to avoid external interference, and it has good aesthetics and safety.

This invention discloses a highly efficient asymmetric synthesis method for aryl propionic acid drug molecules. The method uses racemic aldehydes as substrates and thiamine pyrophosphate-dependent enzymes as biocatalysts. Electrolysis is performed in a diaphragm-free electrolytic cell under conditions of an external redox dielectric and constant current, in a buffer salt solution and nitrogen atmosphere. This method achieves dynamic kinetic oxidation of aldehydes via bioelectrocatalysis, enabling the high-efficiency and high enantioselectivity synthesis of aryl propionic acid drug molecules. The method eliminates the need for redox agents and additives, is simple to operate, operates under mild reaction conditions, and is green and sustainable. This invention applies bioelectrocatalysis to asymmetric synthesis, providing a green and efficient new enzymatic synthetic route for the asymmetric synthesis of aryl propionic acid drug molecules, which has significant application prospects in the pharmaceutical industry.

The utility model belongs to the field of paperboards, and particularly relates to a positioning tool for paperboard slotting, which comprises two mounting seats, two guide rods are fixedly mounted between the two mounting seats, two adjusting seats are sleeved on the two guide rods in a sliding manner, and positioning plates for paperboard positioning are fixedly mounted on the sides, close to each other, of the two adjusting seats; a first threaded hole is formed in the rear side of the adjusting seat, a locking screw is installed in the first threaded hole in a threaded mode and matched with the guide rods, a rotary knob is fixedly installed at the rear end of the locking screw, two fixing seats are fixedly installed on the two guide rods, and positioning seats are installed on the front sides of the fixing seats. A locking mechanism is arranged between the positioning seat and the fixing seat, an adjusting plate is installed on the top of the positioning seat, and an adjusting mechanism is arranged between the adjusting plate and the fixing seat. The paperboard positioning tool is reasonable in design, the purpose of positioning different paperboards can be achieved through adjustment and fixation of the positioning plate, the positioning base and the idler wheels, and therefore the applicability of the positioning tool can be improved.

The utility model relates to road detection technical field discloses a kind of road detection vehicles of being convenient for transportation, including vehicle main body, the front of vehicle main body is fixedly connected with ladder, the upper surface center of vehicle main body is fixedly connected with placing box, the upper portion of placing box is provided with rain cloth, the front end of rain cloth is fixedly connected with magnetic pole, the front and back sides of placing box are fixedly connected with fixed plate, the upper surface left side of fixed plate is fixedly connected with vertical board, the right side surface of vertical board is rotatably connected with rotating rod, the right end of rotating rod is fixedly connected with drive unit. In the utility model, the cooperation of rain cloth, magnetic pole, fixed plate, vertical board, rotating rod, drive unit and limit slot being set, the shielding protection of detection module placed in placing box inside being quickly completed after being disassembled, avoid the step cumbersome caused by need through rope binding, simultaneously, rain cloth can be made to closely adhere to the upper side of detection module.

This utility model discloses a folding stool, including a seat frame, a backrest, and legs. The backrest is rotatably mounted to the seat frame via a first pivot, and the legs are hinged to the seat frame via a first adjustment assembly. The first adjustment assembly has a limit hole, and the seat frame has a positioning hole corresponding to the limit hole. A rotating pin is inserted into the limit hole and the positioning hole. A second adjustment assembly is provided between the backrest and the seat frame. By providing a second adjustment assembly consisting of a pull plate, a toothed plate, a gear, and a spring, the user can easily adjust the backrest angle by holding the pull plate with one hand to separate the toothed plate from the gear. After releasing the pull plate, the spring automatically resets, causing the toothed plate to re-engage with the gear for quick locking. This structure is not only easy to operate but also achieves stepless adjustment, meeting the user's personalized comfort needs and overcoming the shortcomings of traditional pin-type adjustment which has a fixed angle, is laborious to operate, and is prone to wear.

The invention belongs to the technical field of biology, and relates to a pathogen detection technology based on a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system, in particular to a tubercle bacillus detection and activity determination method based on fluorescent T7-CRISPR. The first purpose is to provide a reagent combination for detecting viable tubercle bacillus, and the reagent combination comprises a) a T7 reverse transcription reaction reagent and b) a CRISPR-Cas13a reaction system; a) the T7 reverse transcription reaction reagent comprises a T7 reverse transcription primer and a T7 reverse transcriptase; and b) the CRISPR-Cas13a reaction system comprises specific crRNA, a fluorescence labeled ssRNA reporter, a reaction buffer solution, a Cas13a protein, RNase-Free H2O and a T7 reverse transcriptase. The invention also provides application of the reagent combination in detection of viable tubercle bacillus. The primer combination disclosed by the invention has the advantages of strong specificity and high sensitivity in detection application, can realize rapid detection and visual detection, and is low in detection cost, simple and convenient to operate and suitable for large-scale clinical application.

The invention provides a rock mass structural surface strength prediction method driven by a ground vibration load, and relates to the technical field of rock mass mechanics. The method comprises the following steps: acquiring and integrating geometric and mechanical parameters, seismic oscillation parameters and test and numerical simulation data of a structural plane to form a standardized database; cleaning and standardizing database data, constructing a dynamic attenuation factor, and screening and enhancing key features to obtain initial input features; constructing a physical experience reference model and a machine learning predictor, and training a hybrid prediction model by combining prediction results of the physical experience reference model and the machine learning predictor; verifying the model and quantizing the output uncertainty; inputting the basic data calling model to complete calculation and outputting a result. According to the method, the reliability and adaptability of rock mass structural surface strength prediction are improved, and support is provided for seismic design of geotechnical engineering.

The utility model provides a natural gas mixes hydrogen ratio mixed gas adjusting device, natural gas mixes hydrogen ratio mixed gas adjusting device includes mounting bracket, is fixedly connected with natural gas inlet pipeline and hydrogen inlet pipeline respectively on the mounting bracket, one end of hydrogen inlet pipeline is fixedly connected with hydrogen flowmeter, the hydrogen flowmeter is fixedly connected with hydrogen regulating valve away from one end of hydrogen inlet pipeline, one end of natural gas inlet pipeline is fixedly connected with natural gas flowmeter, the natural gas flowmeter is fixedly connected with natural gas regulating valve away from one end of natural gas inlet pipeline, the other end of natural gas inlet pipeline is fixedly connected with connecting section, the other end of connecting section away from natural gas inlet pipeline is fixedly connected with hydrogen inlet pipeline, the outside wall of connecting section is fixedly connected with adjusting assembly, realizes the flexible adjustment to the flow passage cross -section area through adjusting assembly, effectively adapts the working condition of different flow and pressure, improves the applicability.

The application relates to the battery technical field and discloses a cabinet type battery module, which comprises an outer frame assembly, sliding rail assemblies are arranged on the two sides in the inner part of the outer frame assembly, a battery assembly is clamped between the two sliding rail assemblies, a first fixing bolt is arranged on the back surface of the sliding rail assembly, a second fixing bolt is arranged on the front surface of the sliding rail assembly, the outer frame assembly and the sliding rail assembly are fixed through the second fixing bolt, height positioning is conducted, the movement of the sliding rail assembly in the horizontal direction is positioned through the first fixing bolt, the first fixing bolt is disassembled, the battery assembly is pulled out along the horizontal direction through the front surface of the sliding rail assembly, and the battery assembly is disassembled; the battery assembly can be exposed by pulling the sliding rail assembly after the first fixing bolt is disassembled, so that direct maintenance and replacement can be conducted.

The utility model discloses a kind of stranding head mechanisms for electrical equipment production, including base, transmission module, wire clamping assembly and rotary drive assembly, transmission module realizes the station adjustment of wire clamping assembly;Wire clamping assembly includes rotating platform, wire clamping and switch clamp component, wherein wire clamping is formed double clamping station by two groups of movable clamps with fixed clamp, and wire precision positioning is realized using limiting groove and limiting block, switch clamp component is driven by opening clamp cylinder driving top rod and conical top block to control clamping station opening and closing;It is completed stranding by rotary drive assembly driven rotating platform rotation.This stranding head mechanism solves the problems such as traditional equipment clamping instability, complex structure and poor automation adaptability, with the advantages of clamping stability, transmission accuracy, easy integration, suitable for high-precision, large-batch production of motor, transformer and other equipment winding coil.

The invention relates to the technical field of drilling and production equipment testing tools, in particular to a drill rod bidirectional clamping high-temperature testing clamp which comprises a shell, an outer wall clamping mechanism and an inner wall clamping mechanism, and the outer wall clamping mechanism and the inner wall clamping mechanism are arranged in the shell. The outer wall clamping mechanism comprises a contraction elastic clamping sleeve and a first driving device in driving connection with the contraction elastic clamping sleeve, and the inner wall clamping mechanism comprises an expansion elastic clamping sleeve and a second driving device in driving connection with the expansion elastic clamping sleeve. The contraction elastic clamping sleeve and the expansion elastic clamping sleeve are coaxially arranged in a spaced mode, and a clamping channel used for clamping the end of the drill rod is formed between the inner wall of the contraction elastic clamping sleeve and the outer wall of the expansion elastic clamping sleeve. The first driving device can drive the contraction elastic clamping sleeve to contract in the radial direction of the clamping channel, and the contraction elastic clamping sleeve expands and resets in the radial direction of the clamping channel after being undriven; the second driving device can drive the expansion elastic clamping sleeve to expand in the radial direction of the clamping channel, and the expansion elastic clamping sleeve shrinks and resets in the radial direction of the clamping channel after being undriven.

The application relates to a thread supply adjustable device and a sewing machine. The thread supply adjustable device comprises a first thread supply and take-up mechanism, a second thread supply and take-up mechanism and a driving mechanism. The first thread supply and take-up mechanism is used for intermittently pushing the lower thread of the sewing machine to control the thread supply amount of the lower thread. The second thread supply and take-up mechanism is independently arranged with the first thread supply and take-up mechanism and is used for intermittently pushing the upper thread of the sewing machine to control the thread supply amount of the upper thread. The driving mechanism is in driving connection with the first thread supply and take-up mechanism and the second thread supply and take-up mechanism, and the driving mechanism can actively provide power to the first thread supply and take-up mechanism and the second thread supply and take-up mechanism. The thread supply adjustable device is not only simple to operate, but also can accurately adjust the thread supply and take-up timing of the lower thread and the upper thread, so that the sewing machine can realize ideal stitch effects in actual production, and the sewing quality and the production efficiency are remarkably improved.

The present application relates to the technical field of biology, and discloses a construction method of a Pseudomonas putida suicide vector and application thereof.The construction method of the Pseudomonas putida suicide vector provided by the present application takes a pK18mobsacB vector as a template to obtain a vector skeleton by amplification, and then takes Pseudomonas putida KT2440 bacterial liquid as a template to obtain a strong promoter Prib, upper and lower homologous arms of a gene x to be knocked out, and connects the upper and lower homologous arms to the vector skeleton to obtain the Pseudomonas putida suicide vector.The strong promoter Prib can increase the expression amount of sacB sucrose in the Pseudomonas putida, and enhance the sucrose lethal effect.The Pseudomonas putida suicide vector obtained can be introduced into the Pseudomonas putida, and then the strain with the target gene to be knocked out deleted can be obtained efficiently and accurately through kanamycin positive screening and sucrose negative screening.