Preparation method of core-shell structured synthetic polymer-natural polymer composite fiber

Abstract

The invention discloses a preparation method of a core-shell structured synthetic polymer-natural polymer composite fiber, and the method comprises the steps of: (1) selecting one or several of synthetic polymers to dissolve in a solvent, and conducting stirring until complete dissolution; (2) selecting a natural polymer to dissolve in a solvent, or adding a spinning assistant, and carrying out stirring until complete dissolution; and (3) taking the solution prepared in step (1) as an outer tube spinning solution, adopting the solution prepared in step (2) as an inner tube spinning solution, injecting them into the inner tube and the outer tube of a coaxial spinneret, and performing coaxial electrospinning at room temperature. The core-shell structured nano-fiber prepared by the invention selects the synthetic polymer as the shell layer, and can inhibit water molecules from penetrating the natural polymer as the core layer. The natural polymer as the core layer can more effectively encapsulate active substances to avoid inactivation of the active substances in the presence of an organic solvent, so that the core-shell structured composite fiber can play a good drug sustained release role in the drug carrier field, and an integral activity can be maintained.

Description

Technical field

[0001] The invention relates to the fields of drug slow-release carriers and tissue engineering scaffold materials, in particular to a method for preparing synthetic polymer-natural polymer composite fibers with a core-shell structure. Background technique

[0002] Electrospinning technology has aroused great interest of researchers for many years due to its simple experimental device, lower cost, higher output, easy control and other advantages, and has successfully prepared inorganic, organic and inorganic-organic composites. fiber. However, it is precisely because of the single structure of the spinneret of this method that it can only be used to prepare micro-nano fibers with a single morphology, and it is incapable of preparing nanotubes with complex secondary structures or composite fiber materials with core-shell structures. Compared with a single solid nanofiber, one-dimensional micro-nano materials with a core-shell structure have unique advantages in ma...

Examples

Embodiment 1

[0028] (1) Dissolve polycaprolactone with a molecular weight of 150,000 in a mixed solvent of dichloromethane and DMF with a volume ratio of 4:1 to form a solution with a mass fraction of 8%;

[0029] (2) Use 0.5% Na by mass to volume for silkworm cocoons 2 CO 3 After the solution is degummed, it is dissolved in CaCl with a molar ratio of 1:8:2 at a concentration of 1g / 10mL water 2 , Water and ethanol ternary solvent, then dialysis and suction filtration, the obtained silk fibroin concentration is 3%, and further concentrated to a solution with a mass fraction of 30%;

[0030] (3) Use the solution prepared in the above step (1) as the outer tube spinning solution, and the solution prepared in step (2) as the inner tube spinning solution, respectively, inject into the inner and outer tubes of the coaxial spinneret, and place them at room temperature. The coaxial electrospinning was performed under the following conditions, the spinning parameters were: the flow rate of the inner tube...

Embodiment 2

[0034] (1) Dissolve PLGA (50 / 50) with a molecular weight of 70,000 in a tetrahydrofuran / N,N-dimethylformamide mixed solvent with a volume ratio of 3:1 to form a solution with a mass fraction of 18%;

[0035] (2) Dissolve chitosan in 90% acetic acid solvent to prepare a solution with a mass fraction of 7%;

[0036] (3) Use the solution prepared in the above step (1) as the outer tube spinning solution, and the solution prepared in step (2) as the inner tube spinning solution, respectively, inject into the inner and outer tubes of the coaxial spinneret, and place them at room temperature. The coaxial electrospinning was carried out under the following conditions, the spinning parameters were: the flow rate of the inner tube solution was 0.2 mL / h, the flow rate of the outer tube solution was 0.8 mL / h, the voltage was 15 kV, and the receiving distance was 15 cm.

[0037] In this embodiment, the inner / outer diameter of the inner tube of the coaxial spinneret is 0.5 / 0.7 mm, respectively, a...

Embodiment 3

[0040] (1) Dissolve polycaprolactone with a molecular weight of 80,000 in a chloroform solution and prepare a solution with a mass fraction of 10%;

[0041] (2) Dissolve gelatin in trifluoroethanol solvent to prepare a solution with a mass fraction of 7%;

[0042] (3) Use the solution prepared in the above step (1) as the outer tube spinning solution, and the solution prepared in step (2) as the inner tube spinning solution, respectively, inject into the inner and outer tubes of the coaxial spinneret, and place them at room temperature. The coaxial electrospinning was carried out under the following conditions, and the spinning parameters were as follows: the flow rate of the inner tube solution was 0.5 mL / h, the flow rate of the outer tube solution was 1 mL / h, the voltage was 20 kV, and the receiving distance was 15 cm.

[0043] In this embodiment, the inner / outer diameter of the inner tube of the coaxial spinneret is 0.5 / 0.7 mm, respectively, and the inner / outer diameter of the out...

Technical field

[0001] The invention relates to the fields of drug slow-release carriers and tissue engineering scaffold materials, in particular to a method for preparing synthetic polymer-natural polymer composite fibers with a core-shell structure. Background technique

[0002] Electrospinning technology has aroused great interest of researchers for many years due to its simple experimental device, lower cost, higher output, easy control and other advantages, and has successfully prepared inorganic, organic and inorganic-organic composites. fiber. However, it is precisely because of the single structure of the spinneret of this method that it can only be used to prepare micro-nano fibers with a single morphology, and it is incapable of preparing nanotubes with complex secondary structures or composite fiber materials with core-shell structures. Compared with a single solid nanofiber, one-dimensional micro-nano materials with a core-shell structure have unique advantages in ma...

Examples

Embodiment 1

[0028] (1) Dissolve polycaprolactone with a molecular weight of 150,000 in a mixed solvent of dichloromethane and DMF with a volume ratio of 4:1 to form a solution with a mass fraction of 8%;

[0029] (2) Use 0.5% Na by mass to volume for silkworm cocoons 2 CO 3 After the solution is degummed, it is dissolved in CaCl with a molar ratio of 1:8:2 at a concentration of 1g / 10mL water 2 , Water and ethanol ternary solvent, then dialysis and suction filtration, the obtained silk fibroin concentration is 3%, and further concentrated to a solution with a mass fraction of 30%;

[0030] (3) Use the solution prepared in the above step (1) as the outer tube spinning solution, and the solution prepared in step (2) as the inner tube spinning solution, respectively, inject into the inner and outer tubes of the coaxial spinneret, and place them at room temperature. The coaxial electrospinning was performed under the following conditions, the spinning parameters were: the flow rate of the inner tube...

Embodiment 2

[0034] (1) Dissolve PLGA (50 / 50) with a molecular weight of 70,000 in a tetrahydrofuran / N,N-dimethylformamide mixed solvent with a volume ratio of 3:1 to form a solution with a mass fraction of 18%;

[0035] (2) Dissolve chitosan in 90% acetic acid solvent to prepare a solution with a mass fraction of 7%;

[0036] (3) Use the solution prepared in the above step (1) as the outer tube spinning solution, and the solution prepared in step (2) as the inner tube spinning solution, respectively, inject into the inner and outer tubes of the coaxial spinneret, and place them at room temperature. The coaxial electrospinning was carried out under the following conditions, the spinning parameters were: the flow rate of the inner tube solution was 0.2 mL / h, the flow rate of the outer tube solution was 0.8 mL / h, the voltage was 15 kV, and the receiving distance was 15 cm.

[0037] In this embodiment, the inner / outer diameter of the inner tube of the coaxial spinneret is 0.5 / 0.7 mm, respectively, a...

Embodiment 3

[0040] (1) Dissolve polycaprolactone with a molecular weight of 80,000 in a chloroform solution and prepare a solution with a mass fraction of 10%;

[0041] (2) Dissolve gelatin in trifluoroethanol solvent to prepare a solution with a mass fraction of 7%;

[0042] (3) Use the solution prepared in the above step (1) as the outer tube spinning solution, and the solution prepared in step (2) as the inner tube spinning solution, respectively, inject into the inner and outer tubes of the coaxial spinneret, and place them at room temperature. The coaxial electrospinning was carried out under the following conditions, and the spinning parameters were as follows: the flow rate of the inner tube solution was 0.5 mL / h, the flow rate of the outer tube solution was 1 mL / h, the voltage was 20 kV, and the receiving distance was 15 cm.

[0043] In this embodiment, the inner / outer diameter of the inner tube of the coaxial spinneret is 0.5 / 0.7 mm, respectively, and the inner / outer diameter of the out...