604 results about "Tissue engineering" patented technology

A recombinant humanized collagen and a preparation method therefor. The recombinant humanized type III collagen can be efficiently and stably expressed in three expression systems of yeast, Escherichia coli, and CHO cells, and has the biological activity superior to that of a commercially available collagen product. The recombinant humanized type III collagen has structural characteristics and functional activity similar to those of a natural collagen, and thus has wide application prospects in biomedical materials, tissue engineering products, cosmetics, foods, health care products or drugs, medical instruments, medical cosmetology and the like.

The invention relates to a high-activity recombinant XVII type collagen as well as a rapid preparation method and application thereof, and belongs to the technical field of bioengineering. The amino acid sequence of the high-activity recombinant XVII type collagen is as shown in SEQ ID NO. 1, and the nucleotide sequence of the high-activity recombinant XVII type collagen is as shown in SEQ ID NO. 2. The invention also provides a preparation method and application of the recombinant XVII type collagen. The high-activity recombinant XVII type collagen has good biological activity, has good effects of promoting cell adhesion, cell proliferation and cell migration, and can be compounded with other proteins to form raw materials of tissue engineering products, cosmetics, health care products or medicines. The high-activity recombinant XVII type collagen gene is integrated to different positions of a yeast genome through three different vectors, and the copy number of a target gene is increased, so that the expression quantity of the recombinant XVII type collagen is remarkably increased, and the yield of the high-activity recombinant XVII type collagen is effectively increased.

The invention relates to a recombinant A type humanized XVII type collagen as well as construction and application thereof, the protein is the humanized XVII type collagen which is composed of an intracellular region, a transmembrane region and an extracellular region, and the amino acid sequence is as shown in SEQ No. 1. The hair follicle repairing agent has the advantages of hair follicle repairing, hair regeneration, good biological activities of cell proliferation, cell adhesion and the like, and can be widely applied to the fields of medicines, medical instruments, biological materials, tissue engineering, cosmetics and the like. The gene sequence of the recombinant A type XVII collagen is 100% identical to the corresponding part of the gene sequence of the recombinant A type XVII collagen, so that the recombinant A type XVII collagen has good hydrophilicity and high activity, and has application potential in the fields of biomedical materials, beauty cosmetics, skin care products and the like.

The invention discloses recombinant human I-type collagen and application thereof. The amino acid sequence of the recombinant human I-type collagen is as shown in SEQ ID No: 2. The recombinant human I-type collagen has good cell proliferation promoting activity, cell migration promoting activity and cell adhesion promoting activity (superior to full-length recombinant human I-type collagen or commercially available human collagen) and is easy to produce. The recombinant human I-type collagen can be expected to have the effects of whitening, moisturizing, relieving and repairing, and can be applied to the fields of cosmetics, external medicines, skin care products, health care products, tissue engineering materials, medical instruments and the like.

The invention relates to the technical field of biological tissue engineering, and discloses a nerve-blood brain barrier co-culture model, which comprises a culture chip, and nerves and blood brain barriers respectively constructed in upper culture micropores and lower culture micropores of the culture chip. Wherein a semi-permeable membrane is arranged between the lower-layer culture micropores and the upper-layer culture micropores of the culture chip; the nerves are obtained by inoculating neurons / brain organs obtained by stem cell differentiation into the upper culture micropores for co-culture, and the blood brain barrier is obtained by inoculating brain microvascular endothelial cells obtained by stem cell differentiation into one side of the semipermeable membrane in the lower culture micropores for co-culture. The co-culture model is higher in biomimetic property, and can better simulate the structure and function of the blood brain barrier, for example, the co-culture model has a barrier function closer to the physiological level, the selective permeability of a small molecule drug is matched with the in-vivo permeation condition, and the co-culture model has the function of a small molecule related transporter. The invention further discloses a construction method and application.

The invention relates to the technical field of biology, and particularly discloses a recombinant collagen III and application thereof in preparation of gel. The recombinant collagen III is designed by optimizing functional area sequences of human I-type and III-type collagen, the amino acid sequence is shown as SEQ ID No.2, and the recombinant collagen III has the characteristics of high stability, good hydrophilicity and low immunogenicity. The preparation method comprises the steps of expression vector construction, escherichia coli induced expression, affinity chromatography purification and renaturation. The recombinant collagen III can be prepared into a gel dressing and comprises sodium alginate, methylparaben and other components. Experiments show that the gel can effectively promote cell proliferation and has no cytotoxicity; in a mouse skin injury model, the collagen can relieve inflammation, accelerate wound healing and inhibit scar formation, and the effect of the collagen is superior to that of natural human III-type collagen. The invention provides a safe and efficient novel material for wound repair, and is suitable for medical dressings and tissue engineering.

The invention belongs to the technical field of tissue engineering, and particularly discloses a preparation method of a 3D full-thickness skin model. The preparation method provided by the invention comprises the following steps: constructing a corium layer, constructing an inner epidermal layer through first epidermal inoculation, constructing an epidermal layer through second epidermal inoculation and obtaining a complete 3D full-thickness skin model through co-culture. The 3D full-thickness skin model obtained through the preparation method is distinct in gradation, uniform in thickness, free of shrinkage and stable in quality, the epidermal layer and the corium layer are uniformly fused, and the 3D full-thickness skin model is suitable for skin function research and sample evaluation. Meanwhile, the preparation method provided by the invention is simple in process and convenient to operate, does not need additional reagents or anti-shrinkage tools, can meet the requirements of large-scale industrial production, and has good production feasibility and market application prospects.

The invention discloses hyaluronic acid / alginic acid dual-network hydrogel as well as a preparation method and application thereof. The hyaluronic acid / alginic acid double-network hydrogel comprises a first network and a second network which are not crosslinked with each other, and the first network and the second network are distributed in the hydrogel in an interpenetrating manner; the first network comprises an ionic cross-linked network of alginic acid with cell adhesion sites and calcium ions, and the second network comprises a chemical cross-linked network formed by covalent cross-linking of methacrylic anhydride modified hyaluronic acid. The hyaluronic acid / alginic acid double-network hydrogel disclosed by the invention is short in curing time, supports three-dimensional spreading of cells and formation of capillary lumens, has good mechanical stability, and has good application prospects in the aspects of three-dimensional culture of the cells, tissue engineering construction and the like.

The invention relates to the technical field of synthetic biology, in particular to recombinant XVII type collagen as well as a preparation method and application thereof.The amino acid sequence of the recombinant XVII type collagen comprises a sequence shown in any one of SEQ ID NO.1-SEQ ID NO.3. The recombinant XVII type collagen can be highly expressed in an escherichia coli and pichia pastoris system, the expression system is simple, the cost is low, and the recombinant XVII type collagen is suitable for industrial production. The yield is high; the biological activity is good. The protein can be compounded with other proteins to form raw materials of tissue engineering products, cosmetics, health care products or medicines, so that the protein can be widely applied to the fields of medicines, medical instruments, biological materials, tissue engineering, cosmetics and the like.

The invention discloses a function-enhanced recombinant fibronectin as well as a preparation method and application thereof. The amino acid sequence of the recombinant fibronectin is shown as SEQ ID No.3, and the recombinant fibronectin is formed by fusing a main structural domain FN III8-10 formed by combining human fibronectin mutated into proline at the 171st site with integrin and a main structural domain FN III12-14 formed by combining the human fibronectin with cell factors. The recombinant fibronectin provided by the invention has enhanced cell proliferation and adhesion promoting performance of human fibroblasts, shows excellent cell biological efficacy, can be efficiently expressed in pichia pastoris engineering strains, and has wide application prospects in the fields of skin care, beauty and tissue engineering.

The invention discloses a method for preparing recombinant collagen antioxidant microspheres without a cross-linking agent. The method comprises the following steps: 1, dissolving recombinant collagen and chitosan in deionized water containing glacial acetic acid to serve as a water phase; petroleum ether containing a surfactant is used as an oil phase; 2, injecting the oil phase into the water phase, emulsifying and stirring through a homogenizer, and reducing the temperature of the system to obtain an emulsion; 3, a fixing agent, water and absolute ethyl alcohol are blended and precooled, and fixing liquid is obtained; 4, adding the stationary liquid into the emulsified liquid for curing, and washing the cured microspheres; and 5, transferring into a tannic acid aqueous solution to soak, then washing with deionized water, and freeze-drying to obtain the recombinant collagen microspheres with uniform size. The recombinant collagen microspheres prepared by the method have good dispersity and uniform and controllable size, avoid the toxicity problem of a cross-linking agent, can be applied to the fields of medical instruments, tissue engineering, drug delivery and the like as a filling agent, and have wide commercial application prospects.

According to the method, a one-pot copolymerization method is adopted, MBAA and borax serve as cross-linking agents, zwitterionic polyelectrolyte hydrogel containing SBMA, PVA, THMA and AMPS is prepared, and the zwitterionic polyelectrolyte hydrogel has excellent adhesion performance and biocompatibility and is suitable for the field of biological medicine. According to the method, the physical characteristics of the hydrogel are enhanced due to crosslinking sites and hydrogen bonds of polyvinyl alcohol. The cationic and anionic groups of SBMA improve the adhesion performance, and the reaction monomer THMA provides hydrogen-bond interaction to further promote adhesion, so that the prepared hydrogel adhesive shows excellent adhesion performance and biocompatibility, and is suitable for biological medicine application of wound dressing, drug release systems, tissue engineering and the like.

The present invention provides a yeast recombinant type XVII humanized collagen and its preparation method, in particular, a recombinant humanized collagen expressing the 15th helical region of type XVII collagen, a recombinant engineering bacterium and its preparation method, belonging to the technical fields of genetic engineering and synthetic biology. The present invention provides the full-length sequence of the 15th helical region of type XVII collagen, and mutants are obtained by mutating amino acid residues based on this sequence; for the first time, the present invention expresses the full-length sequence of the 15th helical region of type XVII collagen in Pichia pastoris, establishes a complete set of fermentation processes and purification processes, improves the protein expression level and reduces degradation, and through experiments, it is verified that the obtained protein has biological activities such as cell adhesion activity and cell migration promotion activity that reach or exceed those of natural human collagen, can achieve the purpose of real product application, and can be widely applied in the fields of medicine, medical devices, biological materials, tissue engineering, cosmetics, etc.

The invention belongs to the technical field of biomedical tissue engineering materials, and particularly relates to injectable composite plastic gel and a preparation method thereof. The injectable plastic gel provided by the invention is formed by suspending and compounding the cross-linked sodium hyaluronate gel particles and the biological tissue extracellular matrix particles, the two material particles in the injectable composite plastic gel are uniform in size and controllable in size, other toxic catalysts do not need to be added in the preparation process, and the injectable plastic gel is easy to prepare. The collagen / elastin composite material is rich in collagen and elastin, and shows good biomechanical properties. Meanwhile, the composite plastic gel contains a plurality of bioactive factors, and after the composite plastic gel is injected and filled into a body, the composite plastic gel is slowly degraded and can promote the generation and ingrowth of autologous fat cells and microvessels, so that the balance of degradation and remodeling is achieved. The preparation method of the injectable plastic gel provided by the invention can be widely applied to the fields of plastic surgery, medical beauty and the like.

The invention provides a hydrogel microneedle patch for treating oral ulcer and a preparation method, and belongs to the technical field of biomedical engineering. The technical problems that in the traditional oral ulcer administration process, medicine is difficult to retain at the focus for a long time, is easily scoured by saliva and is influenced by tongue and cheek movement are solved. According to the technical scheme, the method comprises the following steps: 1) designing and preparing a microneedle patch mold; (2) synthesizing drug-loaded polydopamine nano particles; (3) preparing a microneedle patch; and 4) evaluating the performance of the microneedle patch. The invention has the following beneficial effects: on one hand, the combination of the drug-loaded nanoparticles and the hydrogel microneedle array can reduce the dosage through drug sustained release; and on the other hand, the problems of high drug instability, low targeting property, poor bioavailability and hindering treatment effect are effectively solved, and the preparation can be widely applied to the fields of drug sustained release, wound healing, tissue engineering and the like.

The invention discloses hydrogel for improving photocuring 3D printing precision and application of the hydrogel. The invention discovers that pigments such as thioflavin T, alizarin yellow R, methylene blue and / or purple cabbage and the like are added into ink containing photo-crosslinking hydrogel such as methacrylated gelatin (GelMA) and the like as a photo-curing stabilizer, so that the photo-curing 3D printing precision can be improved. On the basis, the hydrogel ink capable of improving the photocuring 3D printing precision is provided, and the photocrosslinking hydrogel is mixed with the photoinitiator and the photocuring stabilizer in proportion, so that an entity obtained by printing through the hydrogel ink has higher precision. In addition, the hydrogel ink for 3D printing is easy to operate and high in practicability, and construction of repair materials needed in the biomedical field such as tissue engineering is facilitated.

The invention provides a preparation method and application of medical grade agarose with different molecular weights, and belongs to the technical field of biomedical materials. According to the invention, the crude agarose product is treated by DMSO, EDTA-Na2 and DEAE-cellulose, and then the acid concentration, the acid treatment temperature, the acid treatment time and the like in the acid treatment stage are finely regulated, so that the medical-grade agarose with medium-high molecular weight (3000-100000), medium molecular weight (3000-3000) and low molecular weight (1t, 3000) is successfully prepared. The medium-high molecular weight and medium molecular weight medical grade agarose provided by the invention has excellent gel performance, adjustable mechanical strength and injection performance, and is suitable for the fields of skin implants, gel dressings, tissue engineering and the like; the low-molecular-weight medical grade agarose shows good water solubility, fluidity and oxidation resistance, and has significant application potential in the fields of medical skin care products and functional dressings.

The invention relates to hydrogel capable of regulating stem cell differentiation as well as a preparation method and application thereof, and belongs to the technical field of biological tissue medical engineering. According to the preparation method disclosed by the invention, the dECM-ODex hydrogel of which the rigidity and viscoelasticity can be independently adjusted is synthesized by utilizing an acellular matrix (dECM) and oxidized dextran (ODex). The dECM contains a large amount of collagen, the collagen forms a main gel network skeleton during polymerization, a weak Schiff base reaction exists between the dECM and ODex, and the mechanical properties, including viscoelasticity and rigidity, of the dECM-ODex hydrogel can be adjusted by keeping the concentration of the dECM hydrogel and adjusting the number of aldehyde groups (namely the molar ratio of NaIO4 to Dex and the concentration of ODex) of the ODex at the same time. And a liver tissue mechanical environment is simulated. The synthesized dECM-ODex hydrogel can be used for regulating and controlling the differentiation of stem cells and treating liver diseases. The invention provides a new tissue engineering material selection for the treatment of liver diseases.

The invention discloses an extracellular matrix-imitated anti-inflammatory nerve repair-promoting conductive hydrogel as well as a preparation method and application thereof, and belongs to the technical field of high polymer materials. The preparation method of the conductive hydrogel comprises the following steps: preparing an MXene nanosheet dispersion liquid, and sequentially adding polyphenol and magnesium ions into the MXene nanosheet dispersion liquid to obtain a modified nanosheet solution; mixing the modified chitosan with polyvinyl alcohol to obtain a solution A; mixing and reacting the modified nanosheet solution with phenylboronic acid modified gelatin to obtain a solution B; mixing the solution A and the solution B for reaction, refrigerating at low temperature, and unfreezing to obtain the conductive hydrogel. The hydrogel prepared by the invention has excellent conductivity, and the obtained conductive hydrogel has the functions of resisting inflammation and promoting nerve repair. The bionics method not only improves the diversity and functionality of biological materials, but also provides new possibility for future tissue engineering and regenerative medicine.

The invention provides a mechano-electric coupling hydrogel microsphere with stable mechanical property and low loss transduction as well as a preparation method and application thereof, and belongs to the technical field of tissue engineering bioremediation materials. According to the invention, supramolecular engineering and microfluidic technologies are utilized, and a slip ring structure polyrotaxane and conductive polypyrrole network is introduced to construct stress-electric coupling hydrogel microspheres. The molecular slippage mechanism of the slip ring structure stores and releases mechanical energy to reduce mechanical loss, and the conjugated pi-electron movement in the conductive network improves the electron transfer efficiency in the microsphere, so that the loss in stress-electricity conversion is reduced for the first time. Compared with the traditional piezoelectric hydrogel microspheres, the stress-electric coupling efficiency of the low-dissipation microspheres is improved by 2.3 times, and the energy dissipation rate is reduced to 43%. The mechano-electric coupling hydrogel microspheres can reduce cartilage injury of rats, improve behavior results and promote treatment of osteoarthritis by recovering low-loss transduction between tissues.

The invention provides a preparation method and application of recombinant humanized III-type collagen F3. The collagen peptide comprises n amino acid sequences as shown in SEQ ID NO. 1; or the collagen peptide comprises n amino acid sequences which have more than 95% of identity with the amino acid sequence as shown in SEQ ID NO.1, n is 2-20, preferably, n is 4-15, more preferably, n is 5-15, and more preferably, n is 6-10. The invention also provides a preparation method and application of the collagen peptide. The collagen peptide has stable high expression, good hydrophilicity, stability, purity and biological activity, and can be further developed into collagen-containing products such as tissue engineering products, cosmetics, health care products, food or pharmaceutical compositions.

The invention provides a fusion protein for promoting proliferation of hair follicle stem cells as well as a preparation method and application of the fusion protein. The fusion protein is formed by fusion expression of a plurality of collagen structural domains capable of being combined with cell integrin and / or specific structural domains from certain functional proteins screened from collagen XVII and III type collagen. Nucleic acid molecules for coding the fusion protein are subjected to codon optimization and then recombined and stably expressed in escherichia coli to obtain the fusion protein. The fusion protein has excellent performance of promoting proliferation and migration of human hair follicle stem cells and human fibroblasts, can be used for repairing skin and hair follicle tissues and improving resistance of skin and hair follicles, and is suitable for popularization and application in the fields of beauty and skin care, medical equipment, tissue engineering and the like.

The utility model relates to the technical field of biological tissue engineering, in particular to an organ chip platform integrated with optical tweezers, which is used for assembling and culturing three-dimensional cell tissues and comprises an organ chip with optical transparency and holographic optical tweezers integrated with the organ chip. The organ chip is horizontally arranged and comprises an upper-layer plate, a middle-layer plate and a lower-layer plate which are sequentially stacked, the middle-layer plate is provided with at least two first culture pools used for inducing and differentiating stem cells into different types of cells and at least one second culture pool used for assembling and culturing the different types of cells, and the lower-layer plate is provided with at least two second culture pools used for assembling and culturing the different types of cells. The channel is connected with the first culture pond and the second culture pond; and an objective lens of the holographic optical tweezers faces the top surface or the bottom surface of the organ chip and is used for capturing, moving and assembling cells in the first culture pool or the second culture pool. According to the utility model, the dependence on a micro-pattern processing technology can be reduced, the cost is reduced, and the regulation and control capability on an organoid tissue structure is improved.

The invention relates to the technical field of tissue engineering, in particular to a 3D printing platform integrated with holographic optical tweezers and a bionic tissue construction method.The printing platform comprises a holographic optical tweezers module used for forming an optical trap array in an objective lens focal region of the holographic optical tweezers module; the charging barrel is used for storing biological materials and can move between a storage position and a working position; the printing base can move between the feeding station and the optical tweezer control station; the objective table is installed on the printing base and can move in the horizontal direction and the vertical direction; the light curing device is fixed on the objective table and is used for emitting light to induce the biological material to be cured; the cell culture plate is fixed on the objective table and stacked on the photocuring device; the cell culture plate is provided with a printing chamber and a cell chamber, and the printing chamber and the cell chamber are communicated through a channel. A macroscopic ordered three-dimensional structure can be constructed, and ordering and patterning of microscale organisms in the macroscopic three-dimensional structure are also realized.

The present invention belongs to the technical field of optimized coding gene, and discloses a recombinant human type I collagen, a preparation method thereof and an application thereof, and its amino acid sequence is shown as SEQ ID No.1. The recombinant type I collagen provided by the present invention has a high expression level, has better cell migration promotion, cell proliferation promotion and cell adhesion promotion properties, has high safety, and can be widely applied to fields such as medicine, medical devices, biological materials, tissue engineering, cosmetics and the like.

The invention discloses starch-collagen composite hydrogel as well as a preparation method and application thereof. Collagen is catalyzed by microbial transglutaminase to form a covalent cross-linked network, and meanwhile, hydroxypropyl starch is introduced to construct a physical interpenetrating network through molecular chain penetration and hydrogen-bond interaction; and chondroitin sulfate is further integrated to strengthen the network structure and biological activity through electrostatic interaction. The method is mild in condition and does not need a toxic chemical cross-linking agent, the obtained composite hydrogel has an interpenetrating double-network structure and has excellent mechanical properties, enzymatic degradation resistance and cell affinity, the compression modulus of the composite hydrogel is remarkably improved, the composite hydrogel can keep structural stability for a long time in a collagenase environment, cell adhesion and proliferation can be effectively promoted, and the composite hydrogel has a good application prospect. The hydrogel can be widely applied to tissue engineering scaffolds, wound dressings, drug sustained-release carriers and cartilage repair materials.

The present invention belongs to the technical field of optimized coding gene, and discloses a recombinant human type IV collagen, a preparation method and an application thereof. The amino acid sequence thereof is shown as SEQ ID No.1. The collagen provided by the present invention has been verified by experiments to have a high yield, good cell proliferation promoting performance, no cytotoxicity, high safety, and can be widely applied in the fields of medicine, medical devices, biological materials, tissue engineering and the like.

Provided herein are devices and methods that represent a dual function approach to tissue engineering and regenerative medicine, particularly for bone fracture or segmental defect healing. The devices include an implantable substrate; a first active composition, and a second active composition that comprises one or more oxygen-modulating compositions. The incorporation of dual bioactive compositions on the substrate offers a versatile platform for manipulating cells and modulating the local tissue oxygen environment at the site of implantation.

The invention discloses composite hydrogel bio-ink with a function of space-time delivery of double growth factors as well as a preparation method and application of the composite hydrogel bio-ink, and belongs to the technical field of biomedical materials and tissue engineering. According to the preparation method, imidazolyl-modified methacrylated gelatin and double-bonded PF127 are compounded, and meanwhile, free vascular endothelial growth factors and connective tissue growth factor-loaded PLGA microspheres are entrapped in a system, so that the bio-ink with excellent printability, high adhesion, swelling resistance and space-time delivery function is formed; the bio-ink physically has the advantages of high printing fidelity, low swelling property, high mechanical strength and strong tissue adhesion of a synthetic material; in biology, the system can be used as an intelligent carrier to realize programmed space-time delivery of various growth factors, so that complex cell biological behaviors are accurately arranged, and real regeneration of functional tissues is guided.

The invention relates to the technical field of biomedical materials and tissue engineering, in particular to a bionic skin model for drug transdermal release research. The hydrogel has skin-like epidermis and dermis structures, the epidermis structure is composed of an electrostatic spinning high-molecular polymer, and the dermis structure is composed of an interpenetrating hydrogel skeleton formed by interweaving polysaccharide, protein and lipid. By adjusting the thickness and composition of the bionic skin, the bionic skin can show a transdermal drug release behavior similar to that of traditional in-vitro skin (such as pigskin, human skin and rat skin) in drug transdermal penetration research, so that the bionic skin is suitable for research on transdermal penetration and absorption behaviors of drugs in external preparations such as ointment, patches and skin care products; and an alternative scheme which is more consistent with ethics, stable, economic and good in reproducibility is provided for prescription screening, quality evaluation and the like.