190 results about "Biological property" patented technology

Methods and systems utilizing quantitative imaging are presented herein. Advantageously, the systems and methods of the present disclosure utilize a hierarchical analysis framework that identifies and quantifies biological properties / analytes from imaging data, and then identifies and characterizes one or more pathologies based on the quantified biological properties / analytes. This hierarchical approach using imaging to examine underlying biology as an intermediate for assessing pathology provides numerous analysis and processing advantages over systems and methods configured to directly determine and characterize pathology from underlying imaging data.

Systems and methods for analyzing pathologies utilizing quantitative imaging are presented herein. Advantageously, the systems and methods of the present disclosure utilize a hierarchical analytics framework that identifies and quantify biological properties / analytes from imaging data and then identifies and characterizes one or more pathologies based on the quantified biological properties / analytes. This hierarchical approach of using imaging to examine underlying biology as an intermediary to assessing pathology provides many analytic and processing advantages over systems and methods that are configured to directly determine and characterize pathology from underlying imaging data.

The invention provides a plant lactobacillus YH001 with bacteriostatic activity as well as a screening method and application of the plant lactobacillus YH001. The phytobacterium plantarum YH001 has the taxonomic name of Lactiplantibacillus plantarum, is preserved in the Guangdong Microbial Culture Collection Center, and has the preservation number of GDMCC No: 65693, the preservation number of Lactiplantibacillus plantarum YH001 is CGMCC NO: 65693, and the preservation number is CGMCC NO: 65693. The preservation date is December 30, 2024. The strain has excellent biological characteristics of no hemolysis, acid resistance, cholate resistance and the like, and has wide application potential in the fields of food, medicine and the like. The fermentation supernatant still keeps high bacteriostatic activity after being treated at a high temperature of 121 DEG C, and shows excellent thermal stability. Therefore, the strain has a wide application prospect in research and development of antibacterial agents, food leavening agents and preservative products.

An antibacterial peptide screening framework is constructed on the basis of a protein language model and a biological information method, and particularly, an antibacterial peptide screening model is developed by combining a protein language model screening result with actual biological activity and biological properties of antibacterial peptide through an innovative calculation method; the problems that in the prior art, antibacterial peptide screening is low in efficiency, insufficient in accuracy and the like are effectively solved, the prediction mode of the functional characteristics of the antibacterial peptide is improved, the diversity and novelty of screening results are improved, and an efficient, accurate and flexible new method is provided for screening of the antibacterial peptide.

The invention relates to plant lactobacillus and application thereof. Wherein the gene of the molecular marker for the plant lactobacillus has a nucleotide sequence selected from one of the following sequences: (1) a nucleotide sequence as shown in SEQ ID NO: 24; (2) a nucleotide sequence having at least 97%, 98%, 99% or higher homology with the nucleotide sequence as shown in SEQ ID NO: 24; and (3) a nucleotide sequence with one or more nucleotide sequences, such as 1, 2, 3, 4, 5 or more nucleotide substitutions, deletion or insertion, in the nucleotide sequence as shown in SEQ ID NO: 24. The plant lactobacillus provided by the invention is non-toxic, has good biological characteristics, has a good curative effect on prevention and / or treatment of vaginal infection and related diseases, and avoids the problems of increased drug resistance, high recurrence rate and the like caused by use of antibiotic and other antibacterial drugs during treatment of vaginal infection and related diseases.

The invention discloses ethanol dehydrogenase, recombinant genetically engineered bacteria and application of the ethanol dehydrogenase in asymmetric reduction of prochiral carbonyl compounds, the ethanol dehydrogenase has good biological performance, heterologous expression in Escherichia coli engineering bacteria is achieved, intracellular coenzyme can be directly utilized when whole cells are adopted as a catalyst, and the catalyst has a good application prospect. Zero addition of the coenzyme is realized, the enzyme has the advantages of high activity, high stereoselectivity, wider substrate spectrum and the like, prochiral ketone compounds can be greenly and efficiently catalyzed to be converted into corresponding chiral alcohols, and the problems of low conversion rate, low yield, low e.e. Value, complex reaction process, more byproducts and the like in a traditional chemical method are solved. The ethanol dehydrogenase has good tolerance to isopropanol, and the isopropanol can be used as a hydrogen source to realize the circulation of coenzyme. And the byproduct acetone generated by the enzyme catalytic reaction and the isopropanol remaining in the reaction can be separated and recovered by a technical means, so that the production cost is greatly reduced.

The invention relates to a strain of Chryseobacterium sp. ZJUHULYY1 and application of the Chryseobacterium sp. ZJUHULYY1 in promotion of corn growth and iron absorption, and provides a strain of Chryseobacterium sp. ZJUHULYY1, which is preserved in China Center for Type Culture Collection on April 27, 2025, and has a preservation number of CCTCC NO: M 2025904. The strain of Chryseobacterium sp. ZJUHULYY1 is obtained by separating and purifying from rhizosphere soil of corn, and the strain of Chryseobacterium sp. ZJUHULYY1 is obtained by separating and purifying from rhizosphere soil of corn. The biological characteristics of the strain are as follows: after being cultured in an LB plate culture medium at 30 DEG C for 24 hours, the bacterial colony is round or oval, golden yellow, smooth and glossy in surface, neat in edge and negative in gram staining; when the strain is inoculated to rhizosphere of corn growing in calcareous soil, compared with a control group, the plant height, chlorophyll content, biomass and active iron content of the corn are all remarkably improved, the growth condition of the corn in the calcareous soil is effectively improved, and the absorption and utilization efficiency of the corn on iron elements is improved.

A method, computer program product, and computer system are provided for evaluating carbon sequestration contributions of nature-based assets located in water environments. The method includes: obtaining meta-descriptors of a nature-based asset and a region of a water environment in which the asset is located; mapping dimensions of the nature-based asset; accessing data of monitored physical characteristics of the nature-based asset in the water environment based on the meta-descriptors of the asset; accessing data of monitored carbon concentration in the region of the water environment based on the meta-descriptors of the region; accessing data on monitored physical, chemical, and biological properties of the water environment based on the meta-descriptors of the region; and applying a model to the accessed data to assign a carbon sequestration contribution per unit measurement of the nature-based asset.

The invention discloses a degradable hypha mulching film and a preparation method thereof, and belongs to the field of agricultural mulching materials, and the degradable hypha mulching film is composed of hypha layers and a hypha cross-linking layer clamped between the hypha layers. The mushroom hypha mulching film is prepared by utilizing the characteristics of adhesion, water resistance and heat preservation of mushroom hyphae, and the method specifically comprises the following steps: culturing mushroom liquid strains, spraying the mushroom liquid strains onto plant fiber paper by taking the plant fiber paper as a bottom film, and degrading lignin and cellulose of the fiber paper by the hyphae to form a hypha cross-linked film, so as to prepare the mushroom hypha mulching film. The film has the basic characteristics of a mulching film, the prepared hypha mulching film has the characteristic of being naturally degraded after the crop growth cycle ends, and no residual matter is left in the soil environment in the degradation process. After the mulching film is degraded, the soil fertility can be effectively improved, the physicochemical properties and biological characteristics of soil can be improved, the problem of environmental pollution caused by the use of a traditional mulching film is further reduced, and powerful support is provided for sustainable development of agricultural production.

The invention provides a crocodile source Aeromonas daka argH gene deletion strain, a crocodile source Aeromonas daka argH gene back-filling strain and a construction method and application of the crocodile source Aeromonas daka argH gene deletion strain and the crocodile source Aeromonas daka argH gene back-filling strain, an argH gene recombinant suicide plasmid pRE112-delta argH is constructed by adopting a seamless cloning method, an Aeromonas daka strain is jointed with E.coli WM3064 containing the pRE112-delta argH to obtain the argH gene deletion strain delta argH, and a corresponding gene back-filling strain C delta argH is further constructed. Through biological characteristic analysis, the argH gene deletion reduces synthesis of arginine of Aeromonas dakara and biofilm formation amount, reduces growth rate, proteolytic activity and resistance to crocodile-derived antibacterial peptide, but enhances hemolytic activity, and significantly improves the survival rate of Caenorhabditis elegans, so that the Aeromonas dakara has a good application prospect in treatment of caenorhabditis elegans, and the Aeromonas dakara has a good application prospect in treatment of caenorhabditis elegans. Important reference is provided for further researching the regulation effect of the argH gene in a bacterial pathogenic mechanism and developing related prevention and control strategies.

The invention belongs to the technical field of biology, and particularly relates to bacillus velezensis HALZ676 as well as application and a method of the bacillus velezensis HALZ676 to cowhide fermentative degradation, the bacillus velezensis HALZ676 is separated from preserved pig trotters, and by analyzing biological characteristics, whole genome and metabonomics differences of the bacillus velezensis HALZ676, the cowhide can be efficiently degraded, and the cowhide can be efficiently degraded. It is found that peptide components smaller than 3000 Da in cow leather degradation liquid obtained through fermentation of the strain HALZ676 account for 68% or above, and the cowhide degradation liquid has remarkable antioxidant capacity and antibacterial capacity; the cowhide fermentation liquor is mainly used for promoting conversion and enrichment of antioxidant, anti-inflammatory and anti-cancer metabolites through a linoleic acid metabolic pathway, so that the antioxidant, anti-inflammatory and anti-cancer capacities of the cowhide fermentation liquor are enhanced; the cowhide fermented by the strain HALZ676 can enrich functional active components, and a theoretical basis and a promising strategy are provided for high-valued production of processing byproducts of livestock and poultry skin fermented by probiotic bacillus with high yield of collagenase.

A method for computational drug design includes defining a population of a plurality of compounds. Each compound includes one or more molecular properties. The method includes defining a training set of compounds from the population for which one or more biological properties are known. The method includes selecting, from the population, a subset of one or more compounds that are not in the training set. The method includes determining a subset score of the selected subset based on molecular properties of the one or more compounds in the selected subset, and evaluating the selected subset based on the determined subset score. The subset score is determined based on a frequency of the molecular properties in the population and on a frequency of the molecular properties in a sampled set comprising the training set and the selected subset.

The invention relates to the technical field of agricultural resources and environment, and particularly discloses a rapid fertilization improvement method for newly-reclaimed red soil dry land. The method systematically integrates the core steps of soil diagnosis aluminum toxicity passivation, composite conditioner deacidification, two-stage deep ploughing, organic and inorganic biological synergistic fertilization, soil moisture content regulation and control, earth surface covering, green manure planting and returning to the field and the like. The applied composition comprises a soil composite conditioner, a charcoal-based organic fertilizer and a specific functional microbial agent, wherein the soil composite conditioner comprises modified oyster shell powder, a calcium magnesium phosphate fertilizer, humic acid, polyglutamic acid and the like. The method relates to key processes such as temperature programming calcination modification of raw materials, co-pyrolysis and composite granulation of the materials and the like. The method can synchronously and quickly improve the chemical and biological properties of soil and optimize the physical structure of a plough layer, and has the advantages of high improvement targeting, synergistic interaction of all steps, high fertilizing speed and lasting effect; in addition, the method has the advantages of being coherent in process, high in resource utilization efficiency and easy to implement on a large scale.

The invention relates to a recombinant mussel-like mucoprotein as well as a coding gene and application thereof, and belongs to the technical field of gene engineering and protein engineering. According to the invention, partial functional sequences of different types of mussel mucin are screened, assembled and spliced to obtain a section of brand new mussel mucin-like sequence, and the sequence is optimized by escherichia coli codon preference to construct a high-expression recombinant strain. According to the recombinant strain, the soluble expression quantity of the target protein is remarkably improved by optimizing culture conditions. The recombinant protein does not carry any exogenous tag, and the provided purification method is low in cost, high in yield and suitable for large-scale production. The prepared recombinant mussel-like protein has good adhesiveness and oxidation resistance, and the modified recombinant mussel-like protein is further provided, and the biological performance of the modified recombinant mussel-like protein is better than that of unmodified recombinant mussel-like protein.

The invention discloses a disease-resistant nursery stock planting method based on nursery stock root protection, and relates to the technical field of nursery stock cultivation and planting. The problem that in the prior art, seedling root systems are not pertinently protected, and consequently the disease resistance of the seedling root systems is insufficient is solved. The survival rate and disease resistance of the seedlings are remarkably improved through whole-process refined treatment, the seedling selection link is accurately screened according to biological characteristics, and the seedling root system quality is guaranteed from the source; soil treatment is combined with deep ploughing, composite disinfection and improvement technologies, soil air permeability is improved by 40% or above, pathogenic bacteria are eliminated, root system protection measures and differentiated pruning and soaking schemes are customized according to different nursery stock characteristics, intertillage and soil loosening are matched with ventilation pipeline laying, and the root system growth physical environment is optimized; the multi-parameter sensor network is linked with the root system monitoring device, real-time dynamic regulation and control of the soil environment and the root system growth state are achieved, the disease resistance of the seedlings is enhanced, and the survival rate and the growth quality of the seedlings are improved.

The invention provides a saline-alkali soil improver with a high-activity surface and application, and belongs to the technical field of saline-alkali soil improvement. Under the ultrasonic condition, a sulfur source is pre-loaded into biomass, under the action of sulfate free radicals, the surface of hydrothermal carbon is etched in the hydrothermal reaction process, the specific surface area and porosity are increased, and meanwhile, part of fractured C-C bonds provide active sites for sulfur doping; meanwhile, alginate and Fe < 3 + > are cross-linked to form a hydrogel network, hydrothermal carbon is wrapped, sulfur stability is enhanced, direct contact between the material and soil is reduced, functional groups of the material are protected, efficient ion exchange in the membrane is achieved, and meanwhile the action duration of the material is prolonged. Through a triple mechanism of'exchange desalination-soil agglomeration-nutrient synergism ', physical, chemical and biological characteristics of the saline-alkali soil can be improved, crop growth is promoted, the content of heavy metals in the soil is reduced, carbon immobilization is enhanced, the bioavailability and toxicity of pollutants are reduced, and the structure and function of an ecological system are restored.

The invention relates to III-type collagen, in particular to application of III-type recombinant collagen peptide in a medical beauty filling material. Compared with aerogel obtained by photo-crosslinking only by the III type collagen peptide, the aerogel obtained by photo-crosslinking the III type collagen peptide and the catechol conjugated chitosan has the advantages that the mechanical property and the biological property of the aerogel obtained by photo-crosslinking only by the III type collagen peptide are improved. The III-type collagen peptide is used as a raw material to prepare the aerogel, and the aerogel can be applied to self-filling, has a natural and lasting filling effect and strong stability, and is not easy to deform or shift. The aerogel preparation process provided by the invention is environment-friendly, does not need the steps of high-temperature heating, chemical corrosion, organic solvent elution and the like, and is relatively environment-friendly.

The invention relates to the technical field of sewage treatment, and discloses a multi-index comprehensive evaluation method for performance comparison and selection of a sewage treatment filler, a scoring system including physical properties, chemical properties, biological properties and economic indexes is established, the physical properties include particle size, porosity and mechanical strength, and the chemical properties include adsorbability and ion exchange capacity; the biological properties comprise microbial adhesiveness and biological membrane activity, and the economical efficiency comprises filler cost and operation and maintenance cost. According to the method, multi-dimensional index fusion is adopted, physical, porosity, mechanical strength and chemical Langmuir adsorption models are integrated, biological electron microscope observation biological membrane coverage rate and economical efficiency LCCA analysis indexes are achieved, and full life cycle evaluation is achieved.

The present invention provides Lactiplantibacillus plantarum and a use thereof. A molecular marker gene for Lactiplantibacillus plantarum has a nucleotide sequence selected from one of the following: (1) a nucleotide sequence as shown in SEQ ID NO: 24; (2) a nucleotide sequence having at least 97%, 98%, 99% or higher homology with the nucleotide sequence as shown in SEQ ID NO: 24; and (3) a nucleotide sequence having one or more, e.g., 1, 2, 3, 4, 5, or more, nucleotide substitutions, deletions, or insertions in the nucleotide sequence as shown in SEQ ID NO: 24. The Lactiplantibacillus plantarum provided by the present invention has non-toxicity and good biological characteristics and has a good curative effect on the prevention and / or treatment of vaginal infection and related diseases thereof.

The invention discloses lactobacillus crispatus CML521 and application thereof, and relates to the technical field of microorganisms, and the lactobacillus crispatus CML521 is technically characterized in that the lactobacillus crispatus CML521 is obtained through separation and culture from poultry craw contents, and the lactobacillus crispatus CML521 has remarkable biological characteristics and can be effectively used for preparing medicines for treating poultry candidiasis; the discovery of the lactobacillus crispatus CML521 provides a brand new treatment thought and method for poultry to treat candidiasis, and the newly discovered microbial strain not only can help poultry to resist the infringement of candidiasis, but also is expected to play an important role in the veterinary medicine field, and provides guarantee for poultry health.

The invention provides a composition with vaginal bacteriostasis, the composition comprises chlorhexidine acetate, a Chinese herbal medicine preparation, lactic acid and a lactobacillus fermentation product, the Chinese herbal medicine preparation comprises cortex phellodendri, fructus cnidii, radix sophorae flavescentis and radix stemonae, and the pH value of the composition is 3.8-4.5. The invention also provides a lotion containing the composition and having a vaginal bacteriostasis effect and a preparation method of the lotion, besides the traditional bacteriostasis component chlorhexidine acetate, the traditional Chinese medicine bacteriostasis component, lactic acid and a lactobacillus fermentation product are also added, and all the components have a synergistic effect on vaginal bacteriostasis after being combined, so that the composition has a good effect on vaginal bacteriostasis. The pH value of the lotion is adjusted to 3.8-4.5 by the added lactic acid, so that the normal acidity of the vaginal cavity is not damaged, the growth of vaginal bacillus is facilitated, the lotion does not contain sugar substances such as chitosan and glucose, the growth and reproduction of pathogenic bacteria and trichomonas vaginalis are not facilitated, and the lotion has a special antibacterial function while the biological characteristics of the vaginal cavity are maintained.

Novel compositions of recombinant human CC10 protein have been generated by chemically modifying the pure protein in vitro. Several new synthetic preparations containing isoforms of chemically modified rhCC10 have been generated by processes that utilize reactive oxygen species and reactive nitrogen species. These preparations contain novel isoforms of rhCC10 which have been characterized with enhanced or altered biological properties compared to the unmodified protein. Preparations containing novel isoforms may be used as standards to identify and characterize naturally occurring isoforms of native CC10 protein from blood or urine and ultimately to measure new CC10-based biomarkers to assess patient disease status. These preparations may also be used to treat respiratory, autoimmune, inflammatory, and other medical conditions that are not effectively treated with the unmodified protein.

The application discloses an application method of leguminous green manure combined with growth-promoting bacteria for emission reduction and efficiency increase, and relates to the technical field of crop planting. The method comprises the following steps: sowing black bean seeds in soil inoculated with bacillus velezensis, and culturing black bean plants to the full flowering stage, and then crushing and turning over the black bean plants as green manure. A special bacillus velezensis is separated, which has the effects of bacteriostasis, nitrogen fixation, phosphorus dissolution, potassium solution, biofilm formation and auxin production, and has excellent biological characteristics such as stress resistance, rapid growth and easy separation, and the advantages of easy cultivation, safety, harmlessness and no pollution. Meanwhile, the bacillus velezensis can ferment to produce rich metabolic products, has good antibacterial activity and stress resistance. The bacillus velezensis is particularly suitable for being combined with black beans for green manure cultivation, and can maximize the effects of soil fertilization and gas emission reduction of green manure.

The present invention relates to an ecological cage aquaculture method. The method proposes a cage-based relay aquaculture model for sea cucumbers, sea urchins, and fish, wherein sea cucumbers and sea urchins are mixed in cages during the wintering period, and are combined with green pufferfish aquaculture in the summer. This achieves the comprehensive utilization of cage aquaculture, effectively avoids the risks of aquaculture of sea cucumbers and sea urchins during the high temperature period in summer, saves feeding bait, and, based on the biological characteristics of green pufferfish that feed on mollusks, removes cage attachments in a targeted manner, achieving a biological cycle and biocontrol of pollutants in marine aquaculture, reducing the labor intensity of subsequent cleaning of cage-fouling organisms. The method adopts a "well"-shaped HDPE grid cage to greatly improve the utilization efficiency of the aquaculture device. The method is equipped with an automatic feeding system to solve the problem of difficult and dangerous feeding in large cages. The method feeds bait in a scientific and standardized manner, saving feeding time and manpower input, reducing labor intensity, and greatly improving labor output efficiency.

The present invention discloses a method for culturing wild Ganoderma curtisii. Using a puncher, 7-mm mycelial blocks are obtained by punching at the same radius on a petri dish covered with mycelia of wild Ganoderma curtisii strains, and then inoculated on a culture medium petri dish and cultured in the dark in a constant temperature incubator at 15-30 °C. Compared with the prior art, the optimal culture conditions for the wild Ganoderma curtisii of the present invention are: carbon source starch, nitrogen source yeast powder, pH 9.0, temperature 30 °C. Under such culture conditions, the wild Ganoderma curtisii grows fastest with the best growth vigor and prefers to grow in a slightly alkaline environment; it provides basic research data for the further development and utilization of Ganoderma curtisii resources and also provides basic data reference for the research on the biological characteristics and domestication cultivation of related species of this genus.

The invention relates to the field of soil mite classification, and discloses a soil mite extractor capable of autonomously detecting types and quantity, which comprises an extractor bracket, an extraction funnel, a crawling channel, an alcohol collecting vessel, an insect expelling lamp, a micro-object camera, an image processor and a display. During working, a collected soil sample is placed in the extraction funnel, and the mites are driven to move downwards and enter the crawling channel through light and thermal stimulation provided by the trapping light source located above the extraction funnel by utilizing the biological characteristics of light and heat resistance of the soil mites. The micro-object camera shoots mites passing through the channel in real time and transmits a video stream to the image processor. A trained image detection algorithm is built in the image processor, and the types and the number of mites in the video are automatically recognized. According to the method, the problem that time and labor need to be consumed for manual counting in traditional soil mite recognition is solved, automation and visualization of the extraction and statistical process are achieved, and the working efficiency is remarkably improved.

The invention relates to the field of plant ecological risk assessment, and discloses a foreign mangrove species ecological influence assessment and risk prediction method, which comprises the following steps: performing biological characteristic information investigation and analysis on target foreign mangrove species to construct a multi-dimensional assessment index system, and performing risk judgment on the target foreign mangrove species according to different index score values, given by the system, of the target foreign mangrove species. And evaluating a risk evolution process after the risk judgment, and outputting a management scheme for the risk. According to the method, the comprehensive influence of external mangrove species can be comprehensively and comprehensively evaluated, key risk points are not missed, meanwhile, the management efficiency is improved, and the decision randomness and uncertainty are reduced.

The invention discloses sinorhizium xinjiangense APXJHT24-1 which has the functions of promoting growth and inhibiting aspergillus flavus, and the preservation number of the sinorhizium xinjiangense APXJHT24-1 is CCTCC (China Center for Type Culture Collection) M 2026229. According to the invention, the integration of two major functions of'generation of a characteristic antibacterial volatile matter of dimethyl disulfide 'and'efficient symbiotic nitrogen fixation' on a single strain is realized in Sinkiang Sinkiang rhizobium for the first time. The dimethyl disulfide released by the strain can accurately down-regulate expression of toxin synthesis key genes and spore development core genes in the aspergillus flavus, so that efficient bacteriostasis is realized on the molecular level. Through one-time inoculation, the strain can synchronously achieve the dual purposes of growth promotion and nitrogen fixation and source bacteriostasis at the rhizosphere ecological niche of the peanuts. Especially aiming at the biological characteristics of overground flowering and underground fruiting of peanuts, a lasting biological fumigation protection layer can be formed in a soil microenvironment in a legume development critical period.

Traditional Chinese medicine plant-based medicine and food homology nano-active peptide preparation and its preparation method, belonging to the field of traditional Chinese medicine preparations. The technical key points are to prepare an aqueous solution of micro-crushed plants; add sodium hydroxide to the aqueous solution for alkali treatment; according to the content ratio of different types of sugars in the plants, add glycosidase of the corresponding sugar type and ratio to the alkali-treated solution for sugar hydrolysis; add protease to the sugar-hydrolyzed solution for protein hydrolysis; add sodium tripolyphosphate and disodium EDTA to the protein-hydrolyzed solution and perform ultrasonic treatment to obtain a plant-based nano-active peptide preparation. The effect is that while retaining and improving traditional active ingredients such as the original quality markers of traditional Chinese medicine, the medicine and food homology is prepared into a nano-level active peptide preparation, enabling it to have the unique physical, chemical, and biological properties of nano materials, and can be applied to drugs, foods, or health products.

The invention provides a bacillus high-yield strain for producing riboflavin and application of the bacillus high-yield strain, and belongs to the technical field of microbial fermentation. The bacillus strain is named as VB2-M-230602-66, the preservation unit is China Center for Type Culture Collection, the preservation date is December 1, 2023, and the preservation number is CCTCC NO: M20232421; according to the invention, the strain VB2-M-230602-66 with stable character is obtained by adopting physical mutagenesis separation and combining with shake flask screening of tolerance dose of a resistance marker, riboflavin can be produced through liquid fermentation, and the yield is stable; the bacterial strain has the biological characteristics that the bacterial strain is gram-positive bacteria through gram staining, the bacteria are cultured at 37 DEG C for 18 hours, the thalli are rod-shaped, 0.5-0.8 mu m * 2.0-5.4 mu m and are arranged in a single or paired manner, and bacterial colonies cultured on a solid culture medium are pale yellow, nearly circular, wet in surface, opaque and irregular in edge; the bacillus strain VB2-M-230602-66 provided by the invention is suitable for fermentation production of riboflavin (vitamin B2), has stable production performance, can obtain greater economic benefits, and is worthy of popularization.