7 results about "Shewanella putrefaciens" patented technology

The invention discloses an on-line monitoring method for a shewanella persea biofilm based on a hyperspectral technology, and belongs to the technical field of food detection.The method comprises the steps that firstly, microscopic hyperspectral data of the biofilm in different growth stages are collected under a pure culture system, and after pretreatment, the hyperspectral data are obtained; variance analysis and LASSO, SPA or PLS-VIP and other methods are adopted for composite feature screening, and key feature wavelengths are extracted; constructing and training a qualitative classification model based on the key characteristic wavelengths to realize accurate classification of growth stages; and then migrating the model which is verified to be valid to a food system, performing stage identification on the biofilm on the surface of the food, and finally generating a visual image reflecting the spatial distribution of the biofilm. According to the method, reliable migration from a laboratory to an actual application scene is realized, and non-destructive, dynamic and visual online identification and spatial analysis can be carried out on the growth stage of the biofilm.

The invention discloses shewanella putrefaciens bacteriophage SpuP2 and application thereof. The preservation number of the shewanella putrefaciens bacteriophage SpuP2 is CCTCC (China Center For Type Culture Collection) NO: M 20251492. The method belongs to the brachycauda family. The titer of a lysate prepared based on the bacteriophage is 108-9PFU / mL, and the lysate can be stably applied under the conditions that the temperature is less than or equal to 50 DEG C and the pH is 5-10. The bacteriophage and the preparation thereof can efficiently inhibit the growth of shewanella putrefaciens, significantly inhibit the formation of a biofilm, remove a mature biofilm, and achieve a sterilization rate of 99-99.99% in aquatic products such as grass carp fillets and the like. The preparation is safe and residue-free, can replace a chemical antibacterial agent to be used for rot prevention and control in the food storage, transportation and processing processes, and has good safety and application prospects.

The present application relates to a kind of microbial inoculant, specifically to a kind of microbial inoculant for improving subtropical secondary forest soil quality, belong to the field of biotechnology.The microbial inoculant includes A component, B component and C component;The A component includes Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus mucilaginosus;The B component includes Shewanella putrefaciens, Podospora sp. And Burkholderia sp.;The C component includes superphosphate, adhesive, soil bulking agent and water.The microbial inoculant is generated by the microbial growth-metabolism-death process of microbial residue carbon and nutrient, green, efficiently improve subtropical secondary forest soil organic matter content, phosphorus-solubilizing nitrogen-fixing, improve soil microbial community structure, promote secondary forest positive succession.

The application discloses an antagonistic bacterium of pathogenic shewanella of Apostichopus japonicus and application thereof, and belongs to the technical field of aquatic microorganisms. Gilvus japonicus The antagonistic bacterium is separated from the intestinal tract of healthy Apostichopus japonicus and is identified as Japanese Yellow Sea Oceanic Bacterium (Shewanella japonica), has no beta-hemolysis characteristic, is safe and non-toxic to Apostichopus japonicus, and can significantly antagonize the pathogenic bacterium Shewanella of Apostichopus japonicus skin rot syndrome. The application also discloses application of the antagonistic bacterium, which can be prepared into an aquatic microecological preparation for preventing and treating the skin rot syndrome of Apostichopus japonicus, and is applied through intraperitoneal injection or immersion, so that the survival rate of Apostichopus japonicus under exposure of the pathogenic bacterium is significantly improved. The strain has strong salt and alkali resistance, tolerances to various antibiotics and chemical drugs, and strong environmental adaptability, can be applied to the cultivation of Apostichopus japonicus to replace traditional antibiotics, effectively solves the problems of food safety and environmental pollution caused by the abuse of antibiotics, and has good industrial application prospect.

The invention relates to the technical field of molecular detection of pathogenic microorganisms, in particular to a group of PCR (Polymerase Chain Reaction) primer pairs for detecting pathogenic shewanella putrefaciens of crucian carps, a product and application of the PCR primer pairs. The nucleotide sequences of the PCR primer pair are as shown in SEQ ID NO. 1 and SEQ ID NO. 2. Based on the PCR primer pair, the invention also develops a detection kit which comprises an upstream primer, a downstream primer, 2 * Taq Plus PCR MIX, a standard positive template DNA, a negative control solution and ddH2O. The PCR primer pair provided by the invention fills the blank of detecting the pathogenic shewanella putrefaciens of the crucian carp, has a good amplification effect on detecting the pathogenic shewanella putrefaciens of the target crucian carp, and has remarkable specificity and relatively high detection sensitivity.

The invention discloses a traditional Chinese medicine composition for inhibiting shewanella putrefaciens and application of the traditional Chinese medicine composition, and relates to the technical field of biological medicines. The traditional Chinese medicine composition comprises any two of gallnut, myrobalan and clove. The invention develops the traditional Chinese medicine composition for inhibiting shewanella putrefaciens, the traditional Chinese medicine composition is compounded from any two of gallnut, myrobalan and clove, the traditional Chinese medicine composition shows a synergistic bacteriostatic effect through experimental verification, the bacteriostatic effect is greatly improved compared with that of a single Chinese herbal medicine, the shewanella putrefaciens proliferation can be rapidly inhibited, and the anti-bacterial effect is good. The spreading and pathogenic processes of the bacillus subtilis in aquatic water and aquatic animal bodies are effectively blocked. The traditional Chinese medicine composition depends on natural Chinese herbal medicine raw materials, is low in toxicity and free of residues, can be prepared into a special antibacterial medicine for aquatic products, prevents and controls diseases such as gill rot and enteritis through oral administration or external application, and reduces the death rate of bred animals; the composition can also be prepared into a water body disinfectant to disinfect culture ponds, tools and the like, so that the pathogen transmission path is cut off from the source.