The invention discloses a special black pine rejuvenation matrix which comprises raw materials including, by weight, 500 parts of fermented materials, 50 parts of pine leaf soil and 1 part of additives. The fermented materials, the pine leaf soil and the additives are uniformly mixed into the rejuvenation matrix. The fermented materials include 1000 parts of animal manure, 72 parts of fallen leaves, 0.1 part of zymophyte, 0.15 part of functional bacteria and 2.25 parts of additives. The zymophyte is EM (effective microorganism) bacteria, and the functional bacteria include geotrichum, Xiamen pseudomonas, soil heterotrophic shewanella, nitrogen-fixing bacteria and bacillus subtilis powder with the weight ratio of 1:1:2:1:1. According to the special black pine rejuvenation matrix, the structure of the soil can be improved, nutrient elements such as manganese, zinc, magnesium and potassium in the soil are remarkably increased, healthy growth of a root system is promoted, roots in the root system can be increased into hundreds of roots, the nutrient and water absorption capacity of black pines is greatly increased, the number of mycetes on the roots on the periphery of the black pine root system is remarkably increased, the dead-arm morbidity of the black pines is reduced from original 100% to 37.8%, and disease resistance is remarkably enhanced.