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Shewanella decolorationis

A technology of shewanella decolorationis and decolorizing Shewanella, applied in bacteria and other directions, can solve the problem of not finding the ability of Shewanella to degrade dyes, and achieve the effect of improving the quality of water ecological environment, simple equipment, and reducing chromaticity and COD.

Active Publication Date: 2005-06-01
GUANGDONG INST OF MICROORGANISM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, new strains of this genus have been continuously discovered in the world. Most of these strains have the ability to reduce metals under anaerobic conditions. bacteria

Method used

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  • Shewanella decolorationis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Add Na to 200 mL deionized water 2 HPO 4 .7H 2 O 2.56g, KH 2 PO 4 0.6g, NaCl 0.1g, NH 4 Cl 0.2g, Acid Scarlet GR (C.I.27290) 0.01g, sterilized at 211°C for 20 minutes as a dye culture solution. Inoculate the LB liquid medium from the slant where Shewanella depigmentation was preserved, and activate the bacteria on a shaker at 30°C and 150r / min to make the number of bacteria reach 10 8 Order of magnitude, inoculate the activated bacteria solution in the dye culture solution with an inoculation amount of 1 / 10, and culture it statically at 30°C, observe the color change of the culture solution, take 10mL of the dye culture solution every 30 minutes and centrifuge at 6 000r / min to remove the bacteria The absorbance of Acid Scarlet GR (C.I.27290) in the dye culture solution was measured at 503nm with a DU640 ultraviolet spectrophotometer (BECKMAN), and the dye degradation rate was calculated using the dye culture solution without bacteria as a control. After 2 hours, ...

Embodiment 2

[0026] Add Na to 200 mL deionized water 2 HPO 4. 7H 2 O 2.56g, KH 2 PO 4 0.6g, NaCl 0.1g, NH 4 Cl 0.2g, Acid Scarlet GR (C.I.27290) 0.1g, sterilized at 211°C for 20 minutes as a dye culture solution. Inoculate the LB liquid medium from the slant where Shewanella depigmentation was preserved, and activate the bacteria on a shaker at 30°C and 150r / min to make the number of bacteria reach 10 8 Order of magnitude, inoculate the activated bacteria solution in the dye culture solution with an inoculation amount of 1 / 10, and culture it statically at 30°C, observe the color change of the culture solution, take 10mL of the dye culture solution every 30 minutes and centrifuge at 6 000r / min to remove the bacteria The absorbance of Acid Scarlet GR (C.I.27290) in the dye culture solution was measured at 503nm with a DU640 ultraviolet spectrophotometer (BECKMAN), and the dye degradation rate was calculated using the dye culture solution without bacteria as a control. After 4 days, th...

Embodiment 3

[0028] Add Na to 200 mL deionized water2 HPO 4 .7H 2 O 2.56g, KH 2 PO 4 0.6g, NaCl 0.1g, NH 4 Cl 0.2g, Reactive Brilliant Blue (C.I.612051) 0.01g, sterilized at 211°C for 20 minutes as dye culture solution. Inoculate the LB liquid medium from the slant where Shewanella depigmentation was preserved, and activate the bacteria on a shaker at 30°C and 150r / min to make the number of bacteria reach 10 8 Order of magnitude, inoculate the activated bacteria solution in the dye culture solution with an inoculation amount of 1 / 10, and culture it statically at 30°C, observe the color change of the culture solution, take 10mL of the dye culture solution every 30 minutes and centrifuge at 6 000r / min to remove the bacteria DU640 UV spectrophotometer (BECKMAN) was used to measure the absorbance of reactive brilliant blue (C.I.612051) in the dye culture solution at 592nm, and the dye culture solution without bacteria was used as a control to calculate the degradation rate of the dye. Af...

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Abstract

The present invention relates to one new kind of Shewanella decolorationis and is Shewanella decolorationis CCTCC M 203093. By means of the separation and purification of the active sludge in the waste water treating system of Guangzhou printing and dyeing plant, one new kind of Shewanella decolorationis is obtained. The Shewanella decolorationis can degrade dye in waste printing and dyeing water effectively, raise waste water treating efficiency and improve water ecological environment quality.

Description

technical field [0001] The present invention relates to a new decolorizing Shewanella (Shewanella decolorationis) CCTCC M 203093, which has the ability to degrade dyes with broad spectrum and high efficiency. Background technique [0002] Dye molecule is a kind of artificially synthesized complex aromatic hydrocarbon compound. According to the statistics of C.I. (Color Index) in the United States, there are as many as 10,000 dyes currently in use. They not only have specific colors, but also have complex structures, low biodegradability, and most of them have three effects. These synthetic dyes are difficult to be effectively removed by conventional wastewater treatment methods, causing serious pollution to the water environment. Therefore, the treatment of dye-containing printing and dyeing wastewater and dye wastewater is one of the hot and difficult points of current research. Studying the biodegradation characteristics of chemical dyes is conducive to the correct use of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 许玫英孙国萍郭俊钟小燕曹渭岑英华
Owner GUANGDONG INST OF MICROORGANISM
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