34 results about "Shewanella" patented technology

The invention discloses a synthetic microbial agent for treating uranium-contaminated soil and a preparation method of the synthetic microbial agent. The synthetic microbial agent is prepared from the following bacteria: P.adami, P.megatherium, bacillus subtilis, Artobacter volluticus, symbiotic fungi, Aspergillus niger, sulfate reducing bacteria and Shewanella. The symbiotic fungi are mucor lastein, mucor unimingguminum, mucor circinelloides, mucor dense, rhizopus arrhizus and phylum phthochytrium parasitic fungi. The prepared synthetic microbial agent and synthetic microbial embedded balls are applied to uranium-contaminated soil remediation, the key enzyme activity can be effectively improved, the soil metabolism function is enhanced, meanwhile, exchangeable uranium with high ecological risk is converted into stable and low-harm residual uranium, the mobility and bioavailability of uranium in soil are effectively weakened, and the uranium-contaminated soil remediation effect is improved. Therefore, immobilized remediation of uranium is realized, and a good soil uranium fixing effect is achieved.

The invention discloses an on-line monitoring method for a shewanella persea biofilm based on a hyperspectral technology, and belongs to the technical field of food detection.The method comprises the steps that firstly, microscopic hyperspectral data of the biofilm in different growth stages are collected under a pure culture system, and after pretreatment, the hyperspectral data are obtained; variance analysis and LASSO, SPA or PLS-VIP and other methods are adopted for composite feature screening, and key feature wavelengths are extracted; constructing and training a qualitative classification model based on the key characteristic wavelengths to realize accurate classification of growth stages; and then migrating the model which is verified to be valid to a food system, performing stage identification on the biofilm on the surface of the food, and finally generating a visual image reflecting the spatial distribution of the biofilm. According to the method, reliable migration from a laboratory to an actual application scene is realized, and non-destructive, dynamic and visual online identification and spatial analysis can be carried out on the growth stage of the biofilm.

The present application relates to the field of metabolic engineering and microbial fermentation, and in particular to a recombinant shewanella producing 5-amino levulinic acid and its application. The present application discloses a new 5-amino levulinic acid producing strain, which is obtained by modularly modifying shewanella by synergistically expressing 5-amino levulinic acid production module genes (hemA, hemL and gltX) derived from shewanella, antioxidant module genes (katE, sodB) derived from escherichia coli, and introducing inhibition modules (the inhibition genes are hemB2 and sucA), so that the shewanella can efficiently produce 5-amino levulinic acid, and the yield of 5-amino levulinic acid can be increased by 134.0 times compared with the original strain.

The invention provides a complex microbial inoculant and application thereof in degradation of starch and biofuel cells, and belongs to the cross technical field of environmental biotechnology and bioelectrochemistry. According to the invention, a complex microbial inoculant composed of three microorganisms is constructed through a synthetic biological strategy; the complex microbial inoculant comprises genetically engineered Escherichia coli capable of rapidly converting starch into monosaccharide easy to use, shewanella for bearing upstream metabolites and geobacter for generating electricity, wherein the genetically engineered Escherichia coli is used for rapidly converting starch into monosaccharide easy to use; according to the invention, through precise function division of three strains, a complex degradation and power generation process is decomposed into a plurality of sub-steps catalyzed by special strains, so that an efficient'metabolic assembly line 'is formed, the inhibition of intermediate products is reduced, and the overall energy conversion efficiency is improved; the complex microbial inoculant can be used for degrading starch and preparing microbial fuel cells, realizes wastewater purification and recovery of bioelectric energy at the same time, and has remarkable environmental and economic benefits.

The invention provides a shewanella-cerium oxide nano-cluster compound, a preparation method thereof and application of the shewanella-cerium oxide nano-cluster compound in preparation of anti-radiation drugs, and belongs to the technical field of anti-radiation materials. According to the invention, a shewanella bioelectronic interface is combined with a CeO2 semiconductor nanomaterial, and when CeO2 forms an interface with shewanella and other electroactive bacteria with EET capability, metal-carrier interaction (MSI) can critically regulate and control an electronic structure of a catalytic active site. The shewanella-cerium oxide nano-cluster compound disclosed by the invention can be used for improving ion conversion between Ce < 3 + > and Ce < 4 + >, so that free radicals brought by radiation damage are reduced, and the damage of radiation to an organism is reduced. Besides, the shewanella-cerium oxide nano-cluster compound disclosed by the invention has excellent antioxidant activity, can regulate and control the intestinal microenvironment while efficiently removing various free radicals, has obvious improvement on intestinal protection after radiation irradiation, and can improve the survival rate of biological organisms.

The application discloses an iron-carbon particle, a preparation method and an iron-carbon particle containing shewanella, and relates to municipal sludge preliminary processing to form a precursor of the iron-carbon particle, acid pickling modification of the precursor of the iron-carbon particle to obtain a porous iron-carbon particle, and soaking of the iron-carbon particle in a shewanella solution to obtain the iron-carbon particle containing shewanella. Micro-electrolysis of the iron-carbon particle can promote the degradation capacity of shewanella to pollutants, and the microbial degradation of shewanella and the adsorption and degradation effect of the iron-carbon particle can further improve the removal effect of antibiotics and heavy metals. The production cost is low, the production efficiency is high, and batch production is facilitated.

The invention provides a synthetic microbial community and application thereof in degradation of trehalose and biofuel cells, and belongs to the technical field of synthetic microbiology and biological energy. A synthetic microbial community is constructed by means of synthetic biology, the synthetic microbial community comprises genetic engineering escherichia coli and shewanella, and the synthetic microbial community can realize a complete metabolic pathway from trehalose to acetic acid and an efficient electron transport chain; the method can be used for treating trehalose-rich wastewater or wastes and the like; the synthetic microbial community can be used for a microbial fuel cell, can stably generate current density and power density which are higher than those of a single strain or a natural mixed flora in the microbial fuel cell, and has good practicability.

The application discloses an antagonistic bacterium of pathogenic shewanella of Apostichopus japonicus and application thereof, and belongs to the technical field of aquatic microorganisms. Gilvus japonicus The antagonistic bacterium is separated from the intestinal tract of healthy Apostichopus japonicus and is identified as Japanese Yellow Sea Oceanic Bacterium (Shewanella japonica), has no beta-hemolysis characteristic, is safe and non-toxic to Apostichopus japonicus, and can significantly antagonize the pathogenic bacterium Shewanella of Apostichopus japonicus skin rot syndrome. The application also discloses application of the antagonistic bacterium, which can be prepared into an aquatic microecological preparation for preventing and treating the skin rot syndrome of Apostichopus japonicus, and is applied through intraperitoneal injection or immersion, so that the survival rate of Apostichopus japonicus under exposure of the pathogenic bacterium is significantly improved. The strain has strong salt and alkali resistance, tolerances to various antibiotics and chemical drugs, and strong environmental adaptability, can be applied to the cultivation of Apostichopus japonicus to replace traditional antibiotics, effectively solves the problems of food safety and environmental pollution caused by the abuse of antibiotics, and has good industrial application prospect.

The application discloses a new Schumannella species with antibacterial activity and application thereof, and belongs to the technical field of microorganisms. A strain 10F1B-5-1 is identified as a potential new species of Schumannella (Schumannella), and is classified and named as Schumannella sp. The strain 10F1B-5-1 has been preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.1.16698. The Schumannella 10F1B-5-1 provided by the application has effects on gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) and gram-negative bacteria (Acinetobacter baumannii), provides a strain resource for research and development of new antibiotics, and has good research value and application potential.

Shewanella bacteria are mode electrochemically active bacteria and have bidirectional electron transfer capability, but the reverse electron transfer rate is slower. In order to solve the problem, the invention discloses a method for synchronously constructing an artificial conductive path in shewanella bacteria attachment so as to improve the electron transfer rate of a bacteria-electrode interface and the energy metabolism activity of bacteria. By utilizing the method, after dopamine is subjected to in-situ electropolymerization in the adsorption process of shewanella tamariscina PV-4, polydopamine is formed in a bacterium-electrode interface area, and the current transferred by reverse electrons is improved by 3.46 times. Due to the fact that polydopamine can serve as an artificial conductive path, the reverse electron transfer capacity of shewanella single cells is improved, and the intracellular energy metabolism level is improved through faster electron transfer.

The invention discloses a microbial composite flora for treating aquaculture tail water and application of the microbial composite flora, the microbial composite flora comprises aeromonas bivalve, shewanella, bacillus paralicheniformis and acinetobacter, and the ratio of the viable count of the aeromonas bivalve to the viable count of the shewanella to the viable count of the bacillus paralicheniformis to the viable count of the acinetobacter is (1-3): (2-3.5): (0.5-1.5): (1.5-2.5). Through synergistic interaction among the strains, tricaine mesylate pollutants, total phosphorus, total nitrogen and COD (Chemical Oxygen Demand) in the aquaculture tail water can be effectively degraded, and the strain has excellent properties of low temperature resistance and high salt resistance.

The application provides an electrically-driven biological Fenton system and application thereof, and specifically, a low-voltage electric field is externally applied on the basis of the biological Fenton system, oxygen is generated by electrode reaction, an anaerobic / aerobic cycle is constructed around the electrode, and the biological Fenton system is driven to run. Under anaerobic conditions, Shewanella can utilize respiratory metabolism to transfer electrons in the substrate to iron oxide to obtain cyclic ferrous iron; under the condition of a low electric field, the electrode can generate a certain amount of oxygen, and under aerobic conditions, Shewanella can utilize respiratory metabolism to generate hydrogen peroxide, therefore, the ferrous iron obtained by the anaerobic / aerobic cycle and the hydrogen peroxide can generate a Fenton reaction; in addition, through the active point on the surface of the electrode, the electrically-driven biological Fenton system can more effectively improve the pollutant removal capacity. The electrochemical reaction system is simple to operate, so that the system can be simply and effectively regulated by setting the surface electrochemical parameters, and has a good application prospect in groundwater treatment.

The invention discloses a novel Schumann bacterium species with antibacterial activity and application thereof, and belongs to the technical field of microorganisms. The bacterial strain 10F1B-5-1 is identified as a potential new species of Schumannella, the classification name of the bacterial strain 10F1B-5-1 is Schumannella sp., the bacterial strain 10F1B-5-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the bacterial strain 10F1B-5-1 is CGMCC NO.1. 16698. The bacterial strain 10F1B-5-1 can be used for preparing the Schumannella. The Schumann bacterium 10F1B-5-1 provided by the invention has the effect of resisting gram-positive bacteria (staphylococcus aureus and bacillus subtilis) and gram-negative bacteria (acinetobacter baumannii), provides a strain resource for research and development of new antibiotics, and has better research value and application potential.

The invention provides a method for strengthening azo dye decolorization based on co-culture of shewanella and paracoccus denitrificans, and relates to the technical field of microorganism co-culture and bioremediation. The shewanella can reduce various pollutants through extracellular electron transfer, but cannot effectively utilize acetate as a carbon source. Paracoccus denitrificans can efficiently oxidize acetate, but lacks the ability to deliver electrons to extracellular electron acceptors (such as azo dyes). According to the method, the S. oneidensis MR-1 in a stable phase and the P. denitrifans in a logarithmic growth middle phase are co-cultured according to a certain biomass ratio, so that the anaerobic decolorization efficiency on various azo dye pollutants can be remarkably enhanced. By constructing a symbiotic system based on functional complementation, a new strategy with potential is provided for enhanced removal of azo dye pollution.

The invention relates to a microorganism / polymetallic sulfide composite material as well as a preparation method and application thereof, and belongs to the technical field of crossing of environmental microorganisms and nano functional materials. The preparation method of the microorganism / polymetallic sulfide composite material comprises the following steps: (1) inoculating shewanella into an LB culture medium, and performing shake cultivation to obtain a bacterial solution; (2) centrifuging the bacterial liquid, adding precipitated bacterial sludge into an anaerobic reaction buffer solution, and then adding a water-soluble ferric iron salt solution, a water-soluble cobalt source salt solution and a water-soluble sulfur source salt solution; and carrying out shake cultivation to obtain the microorganism / polymetallic sulfide composite material. Through in-situ generation of the iron-cobalt-sulfur composite material on the surface of shewanella, stable loading and biological drive generation of nanoparticles are realized, so that the electron transfer rate and the system dechlorination efficiency are improved, the reaction life of the material is prolonged, and an efficient and sustainable pollutant removal effect is achieved.

The application discloses a kind of microorganism complex bacterial population for treating aquaculture tail water and application thereof, and the microorganism complex bacterial population includes Aeromonas veronii, shewanella, paralutealidium and acinetobacter, and the ratio of viable bacterial count of Aeromonas veronii, shewanella, paralutealidium and acinetobacter is (1-3):(2-3.5):(0.5-1.5):(1.5-2.5). The strains of the application can effectively degrade tricaine mesylate pollutants, total phosphorus, total nitrogen and COD in aquaculture tail water through synergistic effect, and have excellent properties of low-temperature tolerance and high-salt tolerance.

This invention relates to the field of microbial technology, and more particularly to an alkali-resistant strain of *Shewanella allergensis* and its application in the degradation of ammonia nitrogen in wastewater. The strain is *Shewanella allergensis* DN-SY03, and its classification name is... Alishewanella alkalitolerans DN-SY03, with accession number CCTCC NO: M2026078, is capable of degrading ammonia nitrogen and COD in wastewater. It not only grows normally at low temperatures of 10~25℃ but also achieves highly efficient degradation of ammonia nitrogen and COD in wastewater, providing a new solution for the degradation of ammonia nitrogen and COD in wastewater under low winter temperatures.

The invention relates to the technical field of agriculture, and discloses a microorganism-mineral based composite material as well as a preparation method and application thereof. The microorganism-mineral-based composite material comprises the following components: iron phosphate powder and dissimilatory iron reduction microorganisms, wherein the dissimilatory iron reducing microorganisms comprise at least one of shewanella, geobacter, desulfurization vibrio, clostridium butyricum, geoacidophilus and iron-rich bacteria. The microorganism-mineral-based composite material provided by the invention comprises the mineral component iron phosphate and dissimilatory iron reduction microorganisms, and the dissimilatory iron reduction microorganisms can actively reduce the iron phosphate which is originally stable in chemical property and insoluble into Fe (II) with relatively high solubility, so that the crystal structure of the iron phosphate is destroyed; the phosphate fertilizer can release free PO4 < 3-> which can be absorbed and utilized by plants, the effect of the mineral reduction dissolution process driven by microorganisms is far better than that of simple addition when minerals or microorganisms are independently used, and an efficient phosphorus activation mechanism is formed.

The invention discloses a construction method and application of engineering bacteria for reprogramming biological energy through polyphosphate metabolism. According to the invention, genes related to polyphosphate metabolism are screened, recombinant expression plasmids are constructed and introduced into shewanella through electrotransformation to obtain corresponding engineering bacteria, biological metabolism rates of the engineering bacteria are compared from intracellular polyP content, ATP level and maximum output current, and the engineering bacteria SPK2DK co-expressed by ppk2 gene and nadk gene are obtained through further screening. And comprehensively evaluating the energy metabolism enhancement efficiency. The constructed engineering bacteria significantly improve the intracellular energy metabolism state by reconstructing a polyphosphate metabolic pathway, not only have excellent cofactor regeneration capacity and lactic acid metabolism rate, but also can specifically target a tumor microenvironment, induce macrophages to be polarized to an anti-tumor phenotype by efficiently depleting lactic acid, and improve the anti-tumor activity of the macrophages. And a new design thought and a technical basis are provided for developing an in-vivo treatment preparation with high-efficiency energy integration and immune regulation and control functions.

The invention discloses application of a bioelectricity-Fenton system driven by sulfate reducing strain shewanella in environmental pollutant degradation, and belongs to the technical field of bioelectricity-Fenton. In the constructed biological electro-Fenton system, hydrogen sulfide generated by reducing sulfate by the sulfate reducing strain shewanella participates in the biological electro-Fenton system to realize reduction of Fe < 3 + >, and the generated Fe < 2 + > reacts with hydrogen peroxide generated by electrolysis to synchronously generate hydroxyl free radicals. OH, so that efficient degradation of environmental pollutants is realized. According to the constructed bio-electro-Fenton system, sulfate ions commonly existing in the environment can be efficiently utilized, synchronously generated hydrogen sulfide participates in the Fenton system, pollutants in the environment can be efficiently removed through generated hydroxyl free radicals, pollution is avoided, the degradation cost is low, and the efficiency is high.

The application provides an antibacterial peptide AMP-OUC329 of marine plant Lactobacillus and application thereof, the antibacterial peptide is derived from marine plant Lactobacillus Bac-329, the amino acid sequence is VSGFETLDEDAKKLSNN, the molecular weight is 1866.5 Da, and the antibacterial peptide has thermal stability and acid stability.The application extracts the metabolite of Bac-329 strain by culture, obtains the crude extract of antibacterial peptide, uses the antibacterial activity of water product spoilage bacteria Shewanella and the damage of bacterial microstructure as directional screening index, separates by Sephadex G-25 gel filtration chromatography and TSKgel G2000SWxl reverse phase high performance liquid chromatography, optimizes the separation and purification conditions of antibacterial peptide, and the obtained antibacterial peptide has strong inhibition activity on Shewanella and precise target.

The invention discloses a ferrous sulfide nanoparticle-shewanella hybrid material as well as a preparation method and application thereof, and belongs to the field of biomedical materials. The hybrid material comprises shewanella and iron sulfide nanoparticles which are loaded on the surface of the shewanella and in periplasmic space and are synthesized through biological directional mineralization. The hybrid material provided by the invention can target and retain at a tumor site, and shewanella can effectively relieve an acidic microenvironment of a tumor area by actively metabolizing lactic acid in a tumor microenvironment; the loaded ferrous sulfide nanoparticles can generate a photothermal effect under the excitation of near-infrared light, have excellent Fenton-like catalytic performance and hydrogen sulfide release function, and can realize the synergistic enhancement of photothermal therapy, chemical kinetic therapy and hydrogen sulfide mediated gas therapy. The iron sulfide nanoparticle-shewanella hybrid material provided by the invention can realize a synergistically enhanced anti-tumor effect through multi-mode combined treatment.

The application provides a preparation method of iron-reducing bacteria sulfidation modified zero-valent iron material, and compared with the prior art, the application utilizes a microbial reduction method to co-culture Shewanella with iron powder and a sulfur source to synthesize the sulfidation modified zero-valent iron material, realizes reduction of an iron oxide passivation layer, and realizes modification of iron sulfide and a small amount of extracellular polymers of Shewanella on the surface of the zero-valent iron, wherein raw material cost is low, the synthesis method is green and safe, and environment-friendly; and the obtained iron-reducing bacteria sulfidation modified zero-valent iron material has the advantages of large specific surface area, good dispersity and strong electron transfer capacity, the reduction capacity of the material to pollutants is significantly improved, and the material can realize sustainable and efficient removal of pollutants, can efficiently remove various halogenated organic matters and heavy metal pollutants in water, can be widely applied to wastewater treatment, and has wide practical application potential.

The invention belongs to the technical field of biology, and particularly relates to a method for rapidly screening cell wall targeted antibiotic producing bacteria based on a whole-cell biosensor. According to the invention, Shewanella oneidensis MR-1 or a strain with a PghK / PghR homologous system is taken as a chassis, a natural PghK / PghR two-component system is utilized, a delta blaA delta ampG / pBBR-PblaA-lux high-sensitivity report strain is constructed, and a double-layer plate screening system is developed, so that in-situ, real-time and high-throughput screening of cell wall targeted antibiotic producing bacteria is realized, and the cell wall targeted antibiotic producing bacteria can be obtained. And a sustainable and extensible new method is provided for solving the antibiotic resistance crisis.

The invention relates to the technical field of biological medicine, in particular to a bioactive material for tumor treatment and a preparation method and application thereof. The preparation method comprises the following steps: providing adriamycin-loaded magnesium-iron layered double-metal hydroxide nanoparticles; modifying the nanoparticles with glucose oxidase to obtain a drug-loaded enzyme compound; and dispersing the drug-loaded enzyme compound in water, mixing with shewanella, and carrying out centrifugal washing to obtain the bioactive material. The bioactive material disclosed by the invention has tumor targeting property, and in-vivo targeting can better utilize the metabolic function of bacteria to continuously consume metabolites at tumor parts; the apoptosis of tumor cells can be promoted by utilizing the generation of active oxygen and the release of adriamycin hydrochloride, and the ferroptosis of tumors can be realized by reducing glutathione and accumulating lipid peroxide.

The invention discloses a biological magnetite repairing material for removing U (VI) in underground water and a method for repairing U (VI) pollutants. The repairing material takes irradiation modified natural magnetite and facultative anaerobic shewanella as compound raw material components. The surface of the magnetite subjected to irradiation modification has a large number of hydroxyl groups, U (VI) is pre-enriched through coordination, directional attachment of shewanella is driven by concentration gradient, riboflavin electron shuttles secreted by thalli and Fe < 2 + > on the surface of the magnetite form a synergistic reduction system, U (VI) reduction reaction activation energy is reduced, and the reduction performance of the repair material on uranium is greatly enhanced. According to the repairing material and the repairing method, the U (VI) removal rate and the repairing efficiency can be remarkably improved, and meanwhile it is ensured that a reduction product has good stability. The remediation method is easy to operate, economical and efficient, and a new path conforming to green and sustainable development is provided for uranium mine area groundwater pollution remediation.