137 results about "Shewanella" patented technology

The invention provides a method for repairing lead-polluted soil by the combination of plants and microorganisms. The method comprises the following steps: uniformly scattering Indian leaf mustard seeds into lead-containing soil and watering to keep a moist soil condition; and after 10-15 days, adding a microorganism compound bacterial agent into the Indian leaf mustard root soil when the plants sprout and grow, so as to remove lead in the soil by the combination of the absorption effect on the lead of Indian leaf mustards and the strengthening effect of the microorganism compound bacterial agent, wherein the microorganism compound bacterial agent is prepared by mixing Bacillus mycoides, Shewanella and Pseudomonas aeruginosa in a mass ratio of (3-10) to 1 to 1. According to the method, the operation is convenient, the cost is low, the management is easy, secondary pollution cannot be caused, and the application prospect is wide.

The invention provides a saline-alkaline tolerant compound active microbial flora, which is used to process waste drilling well slurry and oil-containing slurry with a high saline-alkaline content. The provided saline-alkaline tolerant active microbial flora contains shewanella mac donell, pseudomonas, and halomonas, wherein the preferable number ratio of shewanella mac donell to pseudomonas to halomonas is 4:3:1. The provided flora has a strong endurance capacity on pH and salt content, and can well adapt to the various environments of waste slurry in drilling well and soil polluted by petroleum. The additives and harmful substances in the waste slurry do not have a prominent inhibition effect on the active microbial flora. The growth speed of the flora is quick, the activity of the flora is stable, and the adaptability of the flora is strong. The production cost is low. The flora can be applied to the biodegradation of waste slurry in drilling wells, repairmen of environment destroyed by petroleum, and chemical engineering industry. The provided flora can increase the biodegradation efficiency on slurry in drilling wells and further improve the related processing technology.

The invention discloses a high-electricity-generation shewanella bacterium. The high-electricity-generation shewanella bacterium is named as Shewanella oneidensis; the high-electricity-generation shewanella bacterium has been preserved in the typical culture preservation center of China; the preservation serial number of the high-electricity-generation shewanella bacterium is CCTCC No: M2014340; the preservation date is July 16, 2014. The invention further discloses application of the high-electricity-generation shewanella bacterium to electricity generation. By means of the high-electricity-generation shewanella bacterium and the application of the high-electricity-generation shewanella bacterium, a survey direction is provided for the electricity-generation capacity of the shewanella bacterium in an MFC. According to the high-electricity-generation shewanella bacterium, when 20mM sodium lactate serves as an electron donor, the maximum output voltage is as high as 449 mV and is improved by 18.2% compared with that ofan initial bacterial strain MR-1, and the maximum output power density is 160 mW/m<2> and is improved by 82.6% compared with that of the initial bacterial strain MR-1.

The invention discloses a gene chip for detecting ten types of pathogenic bacteria in sea areas. The gene chip comprises a solid phase slide on which a quality control probe and a detection probe are arranged, wherein the nucleotide sequence components of the detection probe are shown in SEQ ID NO.1-SEQ ID NO.27. The gene chip is capable of simultaneously detecting enterobacter cloacae, edwardsiella tarda, streptococcus faecalis, vibrio fortis, vibrio harveyi, vibrio parahaemolyticus, vibrio splendidus, vibrio vulnificus, vibrio anguillarum and shewanella smarisflavi just in the presence of DNA (deoxyribose Nucleic Acid) without need of live bacteria. The gene chip has remarkable advantages of high throughput, parallelism, miniaturization, automation, rapidness, sensitiveness, quantification, small use amount, and the like.

The invention discloses a shewanella putrefaciens CR7 strain of which the preservation number is CGMCC NO.6192. The strain is separated out from cattle manure compost which is kept in a low-temperature environment for a long time; and a microbial inoculum prepared from the strain can be applied to livestock and poultry manure compost such as cattle manure and the like in winter, and has the effects of quickly starting compost fermentation, increasing compost fermentation temperature, shortening compost fermentation period and reducing nitrogen element loss. The microbial inoculum prepared from the strain is used for producing an organic fertilizer in winter, and can omit the heating procedure in the early stage of the composting of the organic fertilizer, shorten the production period of the organic fertilizer, increase the yield and lower the production cost.

The invention relates to the technical field of control of soil nitrogen pollution, in particular to shewanella and application thereof to control of soil nitrogen pollution in a vegetable field. Theshewanella has the collection number of CGMCC No. 16843 and is collected on November 30, 2018. The invention further discloses molecular biological characteristics and a culture method of the strain.The shewanella is verified to have the characteristic of promoting crop growth, and is further used as a soil conditioner for controlling the soil nitrogen pollution in the vegetable field.

The invention discloses a use of Shewanella as a feed additive in the abalone culture. Shewanella (comprising WA64 and WA65 strains) has strong a capability of antagonism of abalone pathogenic bacteria and thus Shewanella can be used as a feed additive for abalone culture. The Shewanella as feed additive for abalone culture has the advantages that Shewanella promotes that juvenile abalones vie with pathogenic bacteria for adhesion sites, maintains a normal microecological balance of the intestinal canal, can colonize in the intestinal canal of an abalone, promotes nutrient absorption, improves immunity thereby improving a growth speed and disease resistance of a Haliotis discus hannai, can replace a chemical drug for disease treatment, and does not damage the ecological environment.

The invention relates to the technical field of bio-energy and discloses a recombinant yeast strain and a microbial mixed bacteria electricity generation method. According to the recombinant yeast strain disclosed by the invention, genes PDC1, PDC5, PDC6, ADHI and ADH4 are knocked-out, and genes LDH, XR, XDH and XKS are inserted. Meanwhile, a yeast-Shewanella mixed bacteria system taking glucose and xylose as a carbon source is constructed, and by modifying brewer's yeasts, the glucose and xylose can be metabolized to produce lactic acid, and the carbon source and electron donors are providedfor shewanella in the MFC, so that available carbon source spectrums of the shewanella are increased, and the recombinant yeast strain has excellent performance on the electrochemical effect and laysa foundation for researching biomass by human beings and identifying new energy conversion manners.

The present invention relates to a biological electrochemical detection method of fumaric acid, and belongs to the technical field of biochemical detection. The method is used for specific detection of the fumaric acid, a bacteria liquid of activate cultured shewanella spp. and a buffer are mixed to use as a reaction liquid of a biochemical electrochemical sensor, a fumaric acid-containing sample is added into the reaction liquid of the biochemical electrochemical sensor, a certain electric potential is controlled, change of output current signals is detected, and the fumaric acid concentration in the sample can be calculated according to the relationship between current response and the fumaric acid concentration change. The detection method does not rely on the large-scale instrument and equipment, reduces the cost of determination and operation difficulty, is free of interference of fumaric acid structural analogues, is high in specificity, high in accuracy, fast and efficient, accurate and reliable in test results, and can be applied to biological sample detection, food and drug detection, and cancer screening and diagnosis.

The invention provides an immobilized algae-lysing microorganism as well as a preparation method and an application thereof. The immobilized algae-lysing microorganism is prepared from raw materials in percentage by mass as follows: 40%-60% of algae-lysing microorganisms, 30%-50% of a carrier and 10%-20% of an immobilization material, wherein the algae-lysing microorganisms are one or more of bacillus, streptomyces, pseudomonas, sphingomonas, mycobacterium and shewanella with algae-lysing activity and are liquid, powdery or granular substances, with the viable count not lower than 1.0*10<10> cfu/g. The immobilized algae-lysing microorganism has a large specific surface area, good adsorption performance, high algae-lysing efficiency and environment adaptability, no secondary risk and the like, particularly has remarkable algae-lysing effects on microcystis, anabaena, aphanizomenon and other typical harmful algae and has better application prospects in the field of bioremediation of eutrophic water.

The invention discloses a special black pine rejuvenation matrix which comprises raw materials including, by weight, 500 parts of fermented materials, 50 parts of pine leaf soil and 1 part of additives. The fermented materials, the pine leaf soil and the additives are uniformly mixed into the rejuvenation matrix. The fermented materials include 1000 parts of animal manure, 72 parts of fallen leaves, 0.1 part of zymophyte, 0.15 part of functional bacteria and 2.25 parts of additives. The zymophyte is EM (effective microorganism) bacteria, and the functional bacteria include geotrichum, Xiamen pseudomonas, soil heterotrophic shewanella, nitrogen-fixing bacteria and bacillus subtilis powder with the weight ratio of 1:1:2:1:1. According to the special black pine rejuvenation matrix, the structure of the soil can be improved, nutrient elements such as manganese, zinc, magnesium and potassium in the soil are remarkably increased, healthy growth of a root system is promoted, roots in the root system can be increased into hundreds of roots, the nutrient and water absorption capacity of black pines is greatly increased, the number of mycetes on the roots on the periphery of the black pine root system is remarkably increased, the dead-arm morbidity of the black pines is reduced from original 100% to 37.8%, and disease resistance is remarkably enhanced.

The invention belongs to the field of bioengineering, and relates to a shewanella putrefaciens phage isolated strain and application thereof. The shewanella putrefaciens phage isolated strain is shewanella putrefaciens phage SppYZU05 with the preservation number being CCTCC NO:M2016717. The invention also discloses bacteria inhibition application of the shewanella putrefaciens phage SppYZU05 as afood additive composition or a biological fresh-keeping agent in food production and storage, particularly, spoilage of fresh water or sea water products in cold chain circulation caused by shewanellaputrefaciens phage multiplication can be inhibited, and the product shelf life is prolonged.

The invention discloses a protoporphyrin PPIX-high-yielding shewanella genetic engineering bacterium and a construction method thereof. Suicide-type knockout plasmids p[delta]hemH1 and p[delta]hemH2 are constructed respectively. The suicide plasmids enter host shewanella by a conjugation way, and ferrous chelatase genes hemH1 and hemH2 of shewanella are subjected to double knockout by a homologousrecombination way; meanwhile, chlorhematin is added by an exogenous way to maintain the growth of the engineering bacterium, a large amount of protoporphyrin PPIX is accumulated and released to the outside of cells.

The invention belongs to the technical field of microorganisms and discloses a compound biological preparation. The compound biological preparation is prepared from rhodopseudomonas palustris, citrobacter freundii, shewanella, pseudomonas putida and bacillus pumilus. The compound biological preparation disclosed by the invention is prepared by matching five strains; all the strains promote each other, and a specific carrier is matched, so that the degradation capability on polychlorinated biphenyls is improved.

The invention provides a biological seaweed fertilizer. The biological seaweed fertilizer is obtained by culturing a seaweed water extracting solution and a bacterial solution, wherein the bacterial liquid comprises halomonas, shewanella, Marinobacter, pseudomonas and nitrate reducing bacteria. Compared with the prior art, the microbial preparation is added into the seaweed water extracting solution for culture, plants can be promoted to absorb nutrient substances in the seaweed water extracting solution, then the quality of fruits and vegetables is improved, the content of soluble solids andVC is increased, fruit coloring is promoted, and the salt tolerance of the plants is improved; the preservation time of the biological seaweed fertilizer can be prolonged, and tropical agricultural areas are convenient to use.

The invention discloses a method for degrading sulfamethoxazole. According to the method, sulfamethoxazole is degraded through the synergistic effect of P.denitrificans and shewanella. Accumulation ofintermediate products is reduced under the interaction of microbial flora, and finally anaerobic denitrification is achieved.

This invention relates to a microbial agent. The microbial agent is characterized by comprising functional bacteria and a protective bacterium, wherein the functional bacteria are composed of shewanella putrefaciens, geobacter metallireducens, trichoderma viride and trichoderma harzianum and the protective bacterium is bacillus subtilis. The invention also relates to a method of improving soil quality. The preparation method comprises the following steps: (1) mixing the functional bacteria, the protective bacterium and bioactive substances in proportion to obtain a composite bacterial agent; and (2) fixing the composite bacterial agent obtained in the step (1) on biochar. The microbial agent prepared by the invention has very excellent effect in improving the content of soil organic matters, is simple in method, low in cost and free from secondary pollution, can synergistically degrade soil organic matter pollution, and has broad market value.