137 results about "Shewanella" patented technology

Butane-utilizing bacteria are used to degrade hydrocarbon pollutants such as trichloroethene (TCE). In-situ or ex-situ techniques may be used to reduce or eliminate hydrocarbon pollutants from liquid, gas and solid sources. In a preferred embodiment, TCE concentrations in various aqueous environments are reduced by contacting a contaminated water source with butane-utilizing bacteria in the presence of oxygen to degrade the TCE by cometabolism or direct metabolism. Suitable butane-utilizing bacteria include Pseudomonas, Variovorax, Nocardia, Chryseobacterium, Comamonas, Acidovorax, Rhodococcus, Aureobacterium, Micrococcus, Aeromonas, Stenotrophomonas, Sphingobacterium, Shewanella, Phyllobacterium, Clavibacter, Alcaligenes, Gordona, Corynebacterium and Cytophaga. The butane-utilizing bacteria have relatively low TCE toxicity in comparison with conventional methane-utilizing bacteria, and demonstrate an improved ability to degrade TCE.

Butane-utilizing bacteria are used to degrade hydrocarbon pollutants such as trichloroethene (TCE). In-situ or ex-situ techniques may be used to reduce or eliminate hydrocarbon pollutants from liquid, gas and solid sources. In a preferred embodiment, TCE concentrations in various aqueous environments are reduced by contacting a contaminated water source with butane-utilizing bacteria in the presence of oxygen to degrade the TCE by cometabolism or direct metabolism. Suitable butane-utilizing bacteria include Pseudomonas, Variovorax, Nocardia, Chryseobacterium, Comamonas, Acidovorax, Rhodococcus, Aureobacterium, Micrococcus, Aeromonas, Stenotrophomonas, Sphingobacterium, Shewanella, Phyllobacterium, Clavibacter, Alcaligenes, Gordona, Corynebacterium and Cytophaga. The butane-utilizing bacteria have relatively low TCE toxicity in comparison with conventional methane-utilizing bacteria, and demonstrate an improved ability to degrade TCE.

The invention provides a method for repairing cadmium-polluted soil by the combination of microorganisms and plants. The method comprises the following steps: uniformly scattering ryegrass seeds into cadmium-containing soil and watering to keep a moist soil condition; and after 10-20 days, adding a microorganism compound bacterial agent into ryegrass root soil when the plants sprout and grow, so as to remove cadmium in the soil by the combination of the absorption effect on the cadmium of ryegrass and the strengthening effect of the microorganism compound bacterial agent, wherein the microorganism compound bacterial agent is prepared by mixing Bacillus cereus, Bacillus subtilis and Shewanella in a wet bacterium mass ratio of (1-10) to 1 to 1. According to the method, the operation is convenient, the cost is low, the management is easy, secondary pollution cannot be caused, and the application prospect is wide.

The present disclosure relates to isolation, identification and application of Shewanella putrefaciens strain LH4:18 that grows, under denitrifying anaerobic conditions, on crude oil as the sole carbon source. This organism assists in oil release from substrate in reservoir simulations when grown on either lactate or peptone as a carbon source. Shewanella putrefaciens strain LH4:18 can be used alone or in concert with other microorganisms to improve oil recovery.

The invention provides a method for repairing lead-polluted soil by the combination of plants and microorganisms. The method comprises the following steps: uniformly scattering Indian leaf mustard seeds into lead-containing soil and watering to keep a moist soil condition; and after 10-15 days, adding a microorganism compound bacterial agent into the Indian leaf mustard root soil when the plants sprout and grow, so as to remove lead in the soil by the combination of the absorption effect on the lead of Indian leaf mustards and the strengthening effect of the microorganism compound bacterial agent, wherein the microorganism compound bacterial agent is prepared by mixing Bacillus mycoides, Shewanella and Pseudomonas aeruginosa in a mass ratio of (3-10) to 1 to 1. According to the method, the operation is convenient, the cost is low, the management is easy, secondary pollution cannot be caused, and the application prospect is wide.

The invention provides a saline-alkaline tolerant compound active microbial flora, which is used to process waste drilling well slurry and oil-containing slurry with a high saline-alkaline content. The provided saline-alkaline tolerant active microbial flora contains shewanella mac donell, pseudomonas, and halomonas, wherein the preferable number ratio of shewanella mac donell to pseudomonas to halomonas is 4:3:1. The provided flora has a strong endurance capacity on pH and salt content, and can well adapt to the various environments of waste slurry in drilling well and soil polluted by petroleum. The additives and harmful substances in the waste slurry do not have a prominent inhibition effect on the active microbial flora. The growth speed of the flora is quick, the activity of the flora is stable, and the adaptability of the flora is strong. The production cost is low. The flora can be applied to the biodegradation of waste slurry in drilling wells, repairmen of environment destroyed by petroleum, and chemical engineering industry. The provided flora can increase the biodegradation efficiency on slurry in drilling wells and further improve the related processing technology.

The present disclosure relates to isolation, identification and application of Shewanella putrefaciens strain LH4:18 that grows, under denitrifying anaerobic conditions, on crude oil as the sole carbon source. This organism assists in oil release from substrate in reservoir simulations when grown on either lactate or peptone as a carbon source. Shewanella putrefaciens strain LH4:18 can be used alone or in concert with other microorganisms to improve oil recovery.

The invention discloses a high-electricity-generation shewanella bacterium. The high-electricity-generation shewanella bacterium is named as Shewanella oneidensis; the high-electricity-generation shewanella bacterium has been preserved in the typical culture preservation center of China; the preservation serial number of the high-electricity-generation shewanella bacterium is CCTCC No: M2014340; the preservation date is July 16, 2014. The invention further discloses application of the high-electricity-generation shewanella bacterium to electricity generation. By means of the high-electricity-generation shewanella bacterium and the application of the high-electricity-generation shewanella bacterium, a survey direction is provided for the electricity-generation capacity of the shewanella bacterium in an MFC. According to the high-electricity-generation shewanella bacterium, when 20mM sodium lactate serves as an electron donor, the maximum output voltage is as high as 449 mV and is improved by 18.2% compared with that ofan initial bacterial strain MR-1, and the maximum output power density is 160 mW / m<2> and is improved by 82.6% compared with that of the initial bacterial strain MR-1.

The invention discloses a gene chip for detecting ten types of pathogenic bacteria in sea areas. The gene chip comprises a solid phase slide on which a quality control probe and a detection probe are arranged, wherein the nucleotide sequence components of the detection probe are shown in SEQ ID NO.1-SEQ ID NO.27. The gene chip is capable of simultaneously detecting enterobacter cloacae, edwardsiella tarda, streptococcus faecalis, vibrio fortis, vibrio harveyi, vibrio parahaemolyticus, vibrio splendidus, vibrio vulnificus, vibrio anguillarum and shewanella smarisflavi just in the presence of DNA (deoxyribose Nucleic Acid) without need of live bacteria. The gene chip has remarkable advantages of high throughput, parallelism, miniaturization, automation, rapidness, sensitiveness, quantification, small use amount, and the like.

The invention discloses a shewanella putrefaciens CR7 strain of which the preservation number is CGMCC NO.6192. The strain is separated out from cattle manure compost which is kept in a low-temperature environment for a long time; and a microbial inoculum prepared from the strain can be applied to livestock and poultry manure compost such as cattle manure and the like in winter, and has the effects of quickly starting compost fermentation, increasing compost fermentation temperature, shortening compost fermentation period and reducing nitrogen element loss. The microbial inoculum prepared from the strain is used for producing an organic fertilizer in winter, and can omit the heating procedure in the early stage of the composting of the organic fertilizer, shorten the production period of the organic fertilizer, increase the yield and lower the production cost.

The invention relates to the technical field of control of soil nitrogen pollution, in particular to shewanella and application thereof to control of soil nitrogen pollution in a vegetable field. Theshewanella has the collection number of CGMCC No. 16843 and is collected on November 30, 2018. The invention further discloses molecular biological characteristics and a culture method of the strain.The shewanella is verified to have the characteristic of promoting crop growth, and is further used as a soil conditioner for controlling the soil nitrogen pollution in the vegetable field.

The invention discloses a method for synthesizing shewanella halitios into god nanoparticles and application of the gold nanoparticles. The method comprises the following steps that firstly, shewanella halitios Z4 cultivated to a stable period is centrifugally collected, the shewanella halitios Z4 is cleaned by deionized water, and then the shewanella halitios Z4 is prepared into a bacterium suspension; secondly, the bacterium suspension in the step one is added into a chloroauric acid solution, sodium lactate is added as an electron donor, a table concentrator carries out shake cultivation, and a nanogold solution is obtained through the reaction; thirdly, the nanogold solution in the step two is centrifugally collected, and is dried for 12 hours to 36 hours at the temperature of 60 DEG C to 90 DEG C to obtain the gold nanoparticles. According to a green synthesis method, no large type equipment is needed, the method is simple, the condition is moderate, hydrogen does not need to be added as the electron donor, cost is low, and the method is safe. The synthetic gold nanoparticle particles can be used as catalysts to catalyz degradation of nitroaniline, and the method can be applied to removal of environment pollutants.

The invention discloses a use of Shewanella as a feed additive in the abalone culture. Shewanella (comprising WA64 and WA65 strains) has strong a capability of antagonism of abalone pathogenic bacteria and thus Shewanella can be used as a feed additive for abalone culture. The Shewanella as feed additive for abalone culture has the advantages that Shewanella promotes that juvenile abalones vie with pathogenic bacteria for adhesion sites, maintains a normal microecological balance of the intestinal canal, can colonize in the intestinal canal of an abalone, promotes nutrient absorption, improves immunity thereby improving a growth speed and disease resistance of a Haliotis discus hannai, can replace a chemical drug for disease treatment, and does not damage the ecological environment.

The invention relates to the technical field of biological energy, and discloses a recombinant plasmid, recombinant shewanella and an electricity generation method of a microbial fuel cell (MFC). A promoter suitable for the shewanella is selected to be connected with an exogenous electron carrier flavins gene, so that the shewanella is enabled to generate more flavis, and the electrochemical performance of the MFC is improved; furthermore, a mutant strain is combined with a porin oprF gene, so that the formation of a biofilm is further improved, the going in and out of the flavins in the shewanella can be promoted, the electron transfer rate is increased, and electrons are better transmitted. Furthermore, biocompatible graphene oxide (GO) is used and can be self-assembled into the three-dimensional multilayer biofilm together with the shewanella, so that the thickness of the biofilm and the anode biocapacity are further greatly improved; the operation is simple and fast, and the electrochemical performance of the MFC is effectively improved.

The invention provides Shewanella putrefaciens capable of effectively degrading acetonitrile and application of Shewanella putrefaciens. The collection number of Shewanella putrefaciens is CGMCC No.10148. The Shewanella putrefaciens can be used for treating petrochemical enterprise sewage water having low acetonitrile concentration, and rivers, lakes and seas polluted by the petrochemical enterprise sewage water, is high in adaptability to the petrochemical enterprise sewage water, and high in biostability, and can be used for effectively removing the low-concentration acetonitrile in the petrochemical enterprise sewage water.

The invention relates to the technical field of bio-energy and discloses a recombinant yeast strain and a microbial mixed bacteria electricity generation method. According to the recombinant yeast strain disclosed by the invention, genes PDC1, PDC5, PDC6, ADHI and ADH4 are knocked-out, and genes LDH, XR, XDH and XKS are inserted. Meanwhile, a yeast-Shewanella mixed bacteria system taking glucose and xylose as a carbon source is constructed, and by modifying brewer's yeasts, the glucose and xylose can be metabolized to produce lactic acid, and the carbon source and electron donors are providedfor shewanella in the MFC, so that available carbon source spectrums of the shewanella are increased, and the recombinant yeast strain has excellent performance on the electrochemical effect and laysa foundation for researching biomass by human beings and identifying new energy conversion manners.

The present invention relates to a biological electrochemical detection method of fumaric acid, and belongs to the technical field of biochemical detection. The method is used for specific detection of the fumaric acid, a bacteria liquid of activate cultured shewanella spp. and a buffer are mixed to use as a reaction liquid of a biochemical electrochemical sensor, a fumaric acid-containing sample is added into the reaction liquid of the biochemical electrochemical sensor, a certain electric potential is controlled, change of output current signals is detected, and the fumaric acid concentration in the sample can be calculated according to the relationship between current response and the fumaric acid concentration change. The detection method does not rely on the large-scale instrument and equipment, reduces the cost of determination and operation difficulty, is free of interference of fumaric acid structural analogues, is high in specificity, high in accuracy, fast and efficient, accurate and reliable in test results, and can be applied to biological sample detection, food and drug detection, and cancer screening and diagnosis.

The invention provides an immobilized algae-lysing microorganism as well as a preparation method and an application thereof. The immobilized algae-lysing microorganism is prepared from raw materials in percentage by mass as follows: 40%-60% of algae-lysing microorganisms, 30%-50% of a carrier and 10%-20% of an immobilization material, wherein the algae-lysing microorganisms are one or more of bacillus, streptomyces, pseudomonas, sphingomonas, mycobacterium and shewanella with algae-lysing activity and are liquid, powdery or granular substances, with the viable count not lower than 1.0*10<10> cfu / g. The immobilized algae-lysing microorganism has a large specific surface area, good adsorption performance, high algae-lysing efficiency and environment adaptability, no secondary risk and the like, particularly has remarkable algae-lysing effects on microcystis, anabaena, aphanizomenon and other typical harmful algae and has better application prospects in the field of bioremediation of eutrophic water.

The invention discloses a special black pine rejuvenation matrix which comprises raw materials including, by weight, 500 parts of fermented materials, 50 parts of pine leaf soil and 1 part of additives. The fermented materials, the pine leaf soil and the additives are uniformly mixed into the rejuvenation matrix. The fermented materials include 1000 parts of animal manure, 72 parts of fallen leaves, 0.1 part of zymophyte, 0.15 part of functional bacteria and 2.25 parts of additives. The zymophyte is EM (effective microorganism) bacteria, and the functional bacteria include geotrichum, Xiamen pseudomonas, soil heterotrophic shewanella, nitrogen-fixing bacteria and bacillus subtilis powder with the weight ratio of 1:1:2:1:1. According to the special black pine rejuvenation matrix, the structure of the soil can be improved, nutrient elements such as manganese, zinc, magnesium and potassium in the soil are remarkably increased, healthy growth of a root system is promoted, roots in the root system can be increased into hundreds of roots, the nutrient and water absorption capacity of black pines is greatly increased, the number of mycetes on the roots on the periphery of the black pine root system is remarkably increased, the dead-arm morbidity of the black pines is reduced from original 100% to 37.8%, and disease resistance is remarkably enhanced.

The invention discloses a shewanella-smarisflavi-resistant egg yolk antibody and a preparation method thereof. The shewanella-smarisflavi-resistant egg yolk antibody is obtained by immunizing a laying hen by using inactivated shewanella smarisflavi as an antigen, collecting eggs of the immunized laying hen, separating yolk to obtain a water-soluble component, and separating and purifying to obtain the shewanella-smarisflavi-resistant egg yolk antibody. The shewanella-smarisflavi-resistant egg yolk antibody has features of good specificity and high titer, is used for preventing diseases caused by shewanella smarisflavi and immunological detection, and has good effects. In a preparation process of the egg yolk antibody, blood sampling is not required, immunized animals are not injured, and modern animal rights protection rules are met; and each egg contains more than 100mg of the vibrio-splendidus-resistant egg yolk antibody approximately, and 3g of the vibrio-splendidus-resistant egg yolk antibody can be obtained in a month, and is 10-20 times greater than an antibody extracted from blood of an immunized rabbit. The shewanella-smarisflavi-resistant egg yolk antibody has the advantages of safety, environmental protection, high efficiency, low cost and the like, and is easy to industrialize.

The invention belongs to the field of bioengineering, and relates to a shewanella putrefaciens phage isolated strain and application thereof. The shewanella putrefaciens phage isolated strain is shewanella putrefaciens phage SppYZU05 with the preservation number being CCTCC NO:M2016717. The invention also discloses bacteria inhibition application of the shewanella putrefaciens phage SppYZU05 as afood additive composition or a biological fresh-keeping agent in food production and storage, particularly, spoilage of fresh water or sea water products in cold chain circulation caused by shewanellaputrefaciens phage multiplication can be inhibited, and the product shelf life is prolonged.

The invention discloses a protoporphyrin PPIX-high-yielding shewanella genetic engineering bacterium and a construction method thereof. Suicide-type knockout plasmids p[delta]hemH1 and p[delta]hemH2 are constructed respectively. The suicide plasmids enter host shewanella by a conjugation way, and ferrous chelatase genes hemH1 and hemH2 of shewanella are subjected to double knockout by a homologousrecombination way; meanwhile, chlorhematin is added by an exogenous way to maintain the growth of the engineering bacterium, a large amount of protoporphyrin PPIX is accumulated and released to the outside of cells.

The invention belongs to the technical field of microorganisms and discloses a compound biological preparation. The compound biological preparation is prepared from rhodopseudomonas palustris, citrobacter freundii, shewanella, pseudomonas putida and bacillus pumilus. The compound biological preparation disclosed by the invention is prepared by matching five strains; all the strains promote each other, and a specific carrier is matched, so that the degradation capability on polychlorinated biphenyls is improved.

Immobilized bactericide capable of realizing in-situ restoration of polluted sediment is composed of Hangjin clay 2# loaded conductive microorganisms. A preparation method of the immobilized bactericide includes: 1), pretreating Hangjin clay 2# to obtain granular filler; 2), subjecting conductive microorganism strains to enlarged culture to obtain a to-be-inoculated bacteria solution, proportionally adding Hangjin clay 2# pretreated in the step 1, mixing in an anaerobic condition, standing, and removing supernatant to obtain the immobilized bactericide, wherein the conductive microorganism strains include geobacter sulfurreducens, geobacter metalreducens and shewanella spp.. The invention further discloses the preparation method of the immobilized bactericide and application of the immobilized bactericide in in-situ restoration of the polluted sediment.

The invention provides a biological seaweed fertilizer. The biological seaweed fertilizer is obtained by culturing a seaweed water extracting solution and a bacterial solution, wherein the bacterial liquid comprises halomonas, shewanella, Marinobacter, pseudomonas and nitrate reducing bacteria. Compared with the prior art, the microbial preparation is added into the seaweed water extracting solution for culture, plants can be promoted to absorb nutrient substances in the seaweed water extracting solution, then the quality of fruits and vegetables is improved, the content of soluble solids andVC is increased, fruit coloring is promoted, and the salt tolerance of the plants is improved; the preservation time of the biological seaweed fertilizer can be prolonged, and tropical agricultural areas are convenient to use.

This invention relates to a microbial agent. The microbial agent is characterized by comprising functional bacteria and a protective bacterium, wherein the functional bacteria are composed of shewanella putrefaciens, geobacter metallireducens, trichoderma viride and trichoderma harzianum and the protective bacterium is bacillus subtilis. The invention also relates to a method of improving soil quality. The preparation method comprises the following steps: (1) mixing the functional bacteria, the protective bacterium and bioactive substances in proportion to obtain a composite bacterial agent; and (2) fixing the composite bacterial agent obtained in the step (1) on biochar. The microbial agent prepared by the invention has very excellent effect in improving the content of soil organic matters, is simple in method, low in cost and free from secondary pollution, can synergistically degrade soil organic matter pollution, and has broad market value.

The invention discloses a remediation method and application of a chromium-polluted water body, and the method comprises the following steps: adding biochar-loaded aged nano zero-valent iron and Shewanella MR-1 bacteria into the chromium-polluted water body at the same time, and performing reacting for 120-360 minutes under an anaerobic condition. The preparation method of the biochar-loaded agednano zero-valent iron comprises the following steps: adding biochar into a ferrous sulfate solution, performing uniform mixing, adding a NaBH4 solution, performing continuous stirring, collecting precipitates, performing washing and drying, thereby obtaining the biochar-loaded aged nano zero-valent iron. The nano zero-valent iron is extremely easy to oxidize into aged nano zero-valent iron in theair; after oxidation, the reactive activity is reduced, and the charcoal-loaded aged nano zero-valent iron is coupled with the Shewanella MR-1 bacteria, so that the removal efficiency of hexavalent chromium can be obviously improved, the additional cost required by the nano zero-valent iron in the preparation process and the storage process is avoided, and the method can also be applied to the field of removal of chromium-arsenic composite pollutants in water.