74 results about "Shewanella" patented technology

The invention belongs to the technical field of application of natural extracts, and particularly discloses application of Boswellia amoena essential oil in prevention and treatment of aquatic diseases, in particular application of Boswellia amoena essential oil in inhibition of shewanella algal and vibrio alginolyticus. According to the application, various plant essential oils are tested, the bacteriostatic activity of the plant essential oils on two marine vibrios, i.e., shewanella alginate and vibrio alginolyticus, is researched by a filter paper method and a gradient dilution method, and GC-MS analysis is further performed on several essential oils; according to comprehensive analysis, the frankincense Aman has a remarkable effect on inhibiting shewanella and vibrio alginolyticus, and reference can be provided for application of the frankincense Aman essential oil to prevention and treatment of aquatic diseases.

The invention relates to an alkali-resistant massinia sp., in particular to a separated alkali-resistant massinia sp. P2-6 with broad-spectrum phagocytosis activity on pathogenic bacteria, and the alkali-resistant massinia sp. P2-6 is used for phagocytosis in a bacterium splitting manner, and can be used for preparing the alkali-resistant massinia sp. P2-6 with broad-spectrum phagocytosis activity on pathogenic bacteria. The compound has phagocytosis activity on pathogenic bacteria such as aeromonas veronii, aeromonas hydrophila, aeromonas cainea, pseudomonas aeruginosa, shewanella alginate or photobacterium damsela and the like; the alkali-resistant Marsinia sp. P2-6 disclosed by the invention can be applied to inhibition of pathogenic bacteria in an aquaculture water body; alkali-resisting marssonia sp. P2-6 or a microbial agent thereof is added into an aquaculture feed, so that the organism immunity and / or oxidation resistance and / or disease infection resistance of aquaculture animals can be enhanced; the alkali-resistant Marsinia sp. P2-6 provided by the invention can be used as a feed additive for aquaculture or a biocontrol product for aquaculture, and can also be used for preventing and / or treating diseases of aquaculture animals.

The invention relates to an electroactive microbial system for removing parachlorophenol and a method thereof, and aims to provide an electroactive microbial strain for reducing Fe < 0 > oxidized under the condition of oxygen, generating active Fe < 2 + > and further removing parachlorophenol, so that the defect that Fe < 0 > is easy to passivate when organic pollutants are treated is overcome. In addition, an allogenic material is also introduced, so that the degradation efficiency of the electroactive microorganism enhanced zero-valent iron aeration system on 4-CP is improved; the operation is simple, the cost is low, and meanwhile, the pollution to the environment is avoided; according to the technical scheme, the electroactive microbial system for removing parachlorophenol comprises the following components: 0.08 g / L to 0.60 g / L of zero-valent iron, 0.5 mmol / L to 5.0 mmol / L of allogenic substances and 5 g / L to 40 g / L of electroactive microbial strains, the electroactive microbial strains are shewanella decolorata S12 and shewanella putrefaciens SP200, and the electroactive microbial strains are prepared from the shewanella decolorata S12; the microbial inoculum is at least one of shewanella sp. MR-1 and sulfur reduction geobacter PCA; belongs to the field of environmental microorganisms.

The invention discloses a synthetic microbial agent for treating uranium-contaminated soil and a preparation method of the synthetic microbial agent. The synthetic microbial agent is prepared from the following bacteria: P.adami, P.megatherium, bacillus subtilis, Artobacter volluticus, symbiotic fungi, Aspergillus niger, sulfate reducing bacteria and Shewanella. The symbiotic fungi are mucor lastein, mucor unimingguminum, mucor circinelloides, mucor dense, rhizopus arrhizus and phylum phthochytrium parasitic fungi. The prepared synthetic microbial agent and synthetic microbial embedded balls are applied to uranium-contaminated soil remediation, the key enzyme activity can be effectively improved, the soil metabolism function is enhanced, meanwhile, exchangeable uranium with high ecological risk is converted into stable and low-harm residual uranium, the mobility and bioavailability of uranium in soil are effectively weakened, and the uranium-contaminated soil remediation effect is improved. Therefore, immobilized remediation of uranium is realized, and a good soil uranium fixing effect is achieved.

The invention belongs to the field of environmental biotechnology and nano material application, and relates to a method for accelerating microorganisms to reduce selenite based on immobilized riboflavin. Aiming at the problems of poor stability, difficulty in recovery, non-repeated use and the like of dissolved riboflavin serving as an electron shuttle, riboflavin is grafted on the surface of a multi-walled carbon nanotube through a covalent binding method, and immobilized riboflavin capable of being recycled is constructed. The material can accelerate extracellular electron transfer of shewanella MR-1, and toxic sodium selenite is efficiently reduced into low-toxicity or non-toxic elemental selenium. Results show that the immobilized riboflavin not only maintains high reduction activity of riboflavin, but also has excellent cyclic utilization performance, still maintains high reduction rate after being repeated for multiple times, and effectively reduces operation cost and resource loss. According to the invention, an efficient, economical and sustainable application scene is provided for a microorganism-electron shuttle combined remediation technology, and a new thought is also provided for application of a functional nano material in an environmental biotechnology.

The present invention discloses a method for reducing and fixing Cr(VI) by key functional metal-reducing microorganisms based on a mutualistic symbiotic model: S1, preparing a mixed functional bacterial community electrolyte consisting of conductive bacteria and metal-reducing functional bacterial communities, wherein the conductive bacteria is Shewanella, and the metal-reducing functional bacterial communities include one or more of Bacillus cereus, Ralstonia pietermaritzii, and Paenibacillus alvei; S2, enriching an electroactive biofilm: pouring the prepared mixed functional bacterial community electrolyte into an electrolytic cell in a three-electrode bioelectrochemical system, and applying a positive potential of 100 to 300 mV; S3, reducing and fixing Cr(VI): after generating a mature anode biofilm with a stable current output, pouring out the electrolyte in the electrolytic cell, adding a new electrolyte containing Cr(VI), and applying a negative potential of 500 to 0 mV to the working electrode, so that the Cr(VI) in the electrolyte is reduced and fixed.

The invention relates to an anti-mouse IgG nano antibody and alkaline phosphatase fusion protein as well as a preparation method and application thereof. The anti-mouse IgG nano antibody and alkaline phosphatase fusion protein sequentially comprises an anti-mouse IgG nano antibody, a connecting peptide and shewanella alkaline phosphatase from an amino terminal to a carboxyl terminal. The fusion expression of the anti-mouse IgG nano antibody and shewanella alkaline phosphatase in escherichia coli is designed, so that various defects of a chemical labeling method can be avoided, a high-purity and high-specific activity enzyme-labeled antibody can be prepared, the preparation period of a second antibody can be shortened, the preparation process of the second antibody can be simplified, the stability of batches can be improved, and the dependence on experimental animals can be reduced. The prepared anti-mouse IgG nano antibody and alkaline phosphatase fusion protein can be applied to the field of immunodetection, such as a chemiluminescence immunoassay method, an enzyme-linked immunosorbent assay method, an enzymatic fluorescence immunoassay method, an immunoblotting method and the like.

The invention discloses a method for fixing bacillus subtilis and shewanella in a partitioned manner and application of the method in improving acetic acid / ethanol production through anaerobic fermentation of glucose, and belongs to the technical field of environmental microorganisms and biological catalysis. The preparation method comprises the following steps: by taking corn straw as a raw material, preparing hierarchical pore biochar with gradient pore diameters through a double-size silicon dioxide template and an alkali-salt composite activation process; and spatial isolation planting of double bacteria in a single particle is realized by using a'size matching-step fixing 'strategy. According to the method disclosed by the invention, the inter-bacterial microecological niche is constructed, so that bacterial competition and metabolism interference are effectively avoided, and the bacterial loading amount and the fermentation stability of the microbial inoculum are remarkably improved. When the strain is applied to a glucose anaerobic fermentation system, the total yield of acetic acid and ethanol is increased by 1.6 times compared with that of a free bacterium system. The raw materials are cheap, the process is green, the catalyst can be repeatedly used, an efficient and economical catalyst is provided for biosynthesis of high-value chemicals, and the catalyst has a wide application prospect in the fields of biorefinery and environmental remediation.

The invention discloses construction of a shewanella hydrogengens-CdS nanoparticle hybrid system based on a hydArsp gene and an application of the shewanella hydrogengens-CdS nanoparticle hybrid system. The method comprises the following steps: connecting a hydArsp gene derived from Rhodobacter sphaeroids coded hydrogenase to a vector pYYDT, carrying out ribosome optimization transformation to obtain a plasmid pYYDT-hydArsp, introducing the recombinant plasmid pYYDT-hydArsp into wild shewanella oneidensis MR-1 to obtain a recombinant shewanella engineering bacterium SERS with high hydrogen production efficiency, anchoring CdS nanoparticles on periplasm and an extracellular membrane of a strain in a self-assembly manner, and carrying out high-yield hydrogen production on the recombinant shewanella engineering bacterium SERS. The CdS / SERS high-hydrogen-yield active hybrid system is obtained. The CdS / SERS hybrid system constructed by the invention endows shewanella with light capturing ability, CdS nanoparticles provide photoelectrons for engineering bacteria to promote intracellular reducing power and ATP regeneration, and the hybrid system constructed by the invention can enhance the proton reduction rate of the engineering bacteria and improve the hydrogen production efficiency.

The invention discloses an on-line monitoring method for a shewanella persea biofilm based on a hyperspectral technology, and belongs to the technical field of food detection.The method comprises the steps that firstly, microscopic hyperspectral data of the biofilm in different growth stages are collected under a pure culture system, and after pretreatment, the hyperspectral data are obtained; variance analysis and LASSO, SPA or PLS-VIP and other methods are adopted for composite feature screening, and key feature wavelengths are extracted; constructing and training a qualitative classification model based on the key characteristic wavelengths to realize accurate classification of growth stages; and then migrating the model which is verified to be valid to a food system, performing stage identification on the biofilm on the surface of the food, and finally generating a visual image reflecting the spatial distribution of the biofilm. According to the method, reliable migration from a laboratory to an actual application scene is realized, and non-destructive, dynamic and visual online identification and spatial analysis can be carried out on the growth stage of the biofilm.

The invention belongs to the technical field of microorganisms, and particularly relates to a novel strain of brevibacterium and application thereof. The strain is separated from rapana venosa, is a new species of Brevibacterium, is named as Brevibacterium sp. M-17, is registered and preserved in the China Center for Type Culture Collection (CCTCC), and has the preservation number of CCTCC NO: M 20241860. The Brevibacterium sp. M-17 strain disclosed by the invention has obvious bacteriostatic activity on bacillus subtilis, escherichia coli, shewanella, enterobacter aerogenes, staphylococcus aureus and proteus vulgaris, has obvious antioxidant activity, has alpha-glucosidase inhibitory activity, can generate gelatinase, cellulase and amylase, and can be used for preparing an anti-inflammatory agent. The strain can be applied to preparation of bacteriostatic agents, antioxidants, alpha-glucosidase inhibitors, gelatinase, cellulase and amylase, can also be applied to industrial production instead of the bacteriostatic agents, the antioxidants, the gelatinase, the cellulase and the amylase in a viable bacterium form, and has good development and application prospects.

The invention relates to the technical field of food preservation, in particular to hydrogel containing an electric field response antibacterial material as well as a preparation method and application of the hydrogel. The hydrogel is prepared from the following raw materials in parts by weight: 0.1 to 0.2 part of an electric field response antibacterial material; 2-5 parts by weight of polyvinyl alcohol; 0.5-3 parts by weight of amyloid protein fiber; 30-40 parts by weight of glycerin; and 0.3-0.5 part by weight of sodium chloride. The electric field response antibacterial hydrogel prepared by the invention has the capability of generating active oxygen such as superoxide anions, singlet oxygen and hydroxyl radicals, and has a good in-vitro antibacterial effect on aquatic product putrefying bacteria (shewanella putrefaciens).

The invention provides a platinum adsorption microbial material and a preparation method and application thereof, and belongs to the technical field of precious metal adsorption. The preparation method of the platinum adsorption microbial material comprises the following steps: (1) inoculating shewanella and escherichia coli with metal binding protein genes displayed on the surface into a fermentation culture medium for culture, and carrying out solid-liquid separation to obtain a fermentation inoculant; and (2) fixing the fermentation inoculant obtained in the step (1) on an immobilized carrier to obtain the platinum adsorption microbial material. The platinum adsorption microbial material prepared by the invention not only has the capability of efficiently adsorbing platinum, but also is not easily interfered by other metal ions, and has the effect of selectively adsorbing metal platinum. The platinum adsorption microbial material prepared by the invention is particularly suitable for adsorbing platinum in industrial wastewater, and has important significance on recycling of noble metal resources.

The present application relates to the field of metabolic engineering and microbial fermentation, and in particular to a recombinant shewanella producing 5-amino levulinic acid and its application. The present application discloses a new 5-amino levulinic acid producing strain, which is obtained by modularly modifying shewanella by synergistically expressing 5-amino levulinic acid production module genes (hemA, hemL and gltX) derived from shewanella, antioxidant module genes (katE, sodB) derived from escherichia coli, and introducing inhibition modules (the inhibition genes are hemB2 and sucA), so that the shewanella can efficiently produce 5-amino levulinic acid, and the yield of 5-amino levulinic acid can be increased by 134.0 times compared with the original strain.

The present invention discloses a method for detecting the direct and indirect electron transfer capabilities of biochar. The method comprises the following steps: grinding and sieving the biochar, and then adding ultrapure water to prepare a biochar solution; activating a Shewanella stock solution, inoculating it into a sterilized LB culture medium, placing it in a shaker for cultivation, and repeatedly transferring it multiple times before adding ultrapure water to prepare a Shewanella solution; using an anaerobic state, using a three-electrode system connected to an electrochemical workstation, adding ultrapure water to a glassy carbon cup electrode, setting the voltage, and after the current stabilizes, injecting the Shewanella solution into the glassy carbon cup electrode of a control group, and injecting the Shewanella solution and the biochar solution into the glassy carbon cup electrode of an experimental group. In a current-time curve, the difference in peak current between the experimental group and the control group represents the direct electron transfer capability of the biochar, and the difference in stable current represents the indirect electron transfer capability of the biochar. The method of the present invention is simple and easy to implement, and directly demonstrates the direct and indirect electron transfer capabilities of the biochar.

The invention provides a complex microbial inoculant and application thereof in degradation of starch and biofuel cells, and belongs to the cross technical field of environmental biotechnology and bioelectrochemistry. According to the invention, a complex microbial inoculant composed of three microorganisms is constructed through a synthetic biological strategy; the complex microbial inoculant comprises genetically engineered Escherichia coli capable of rapidly converting starch into monosaccharide easy to use, shewanella for bearing upstream metabolites and geobacter for generating electricity, wherein the genetically engineered Escherichia coli is used for rapidly converting starch into monosaccharide easy to use; according to the invention, through precise function division of three strains, a complex degradation and power generation process is decomposed into a plurality of sub-steps catalyzed by special strains, so that an efficient'metabolic assembly line 'is formed, the inhibition of intermediate products is reduced, and the overall energy conversion efficiency is improved; the complex microbial inoculant can be used for degrading starch and preparing microbial fuel cells, realizes wastewater purification and recovery of bioelectric energy at the same time, and has remarkable environmental and economic benefits.

The invention belongs to the technical field of bio-photonics, and particularly relates to a single molecule detection method for marking shewanella electron transfer and application of the single molecule detection method. The method comprises the following steps: adding a fluorescent probe into a shewanella sample by adopting a single-molecule super-resolution fluorescence microscope technology, specifically binding the fluorescent probe with shewanella cells, exciting the fluorescent probe to emit fluorescence, and carrying out single-molecule fluorescence labeling on the shewanella cells. According to the present invention, the single-molecule super-resolution fluorescence microscope technology and the nile blue specific combination are adopted to achieve the single-molecule-level high temporal-spatial resolution monitoring, the binding site and the dynamic change of the nile blue on the shewanella cells can be accurately observed, the nano-scale resolution visual data can be provided for the research of the cell electron transfer process, and the application prospect is broad. The detection precision is obviously improved; the electron transfer activity of shewanella cells can be analyzed by detecting the change of a Nile blue fluorescence signal, and the method has a wide application prospect in the fields of bioremediation, energy production and the like.

The invention discloses a method for reducing hexavalent chromium by using tire rubber additive-quinone derivative reinforced shewanella, and the method comprises the following steps: S1, preparing an N-(1, 3-dimethylbutyl)-N '-phenyl p-benzoquinone concentrated solution; s2, a culture medium is prepared, Shewanella oneidensis MR-1 is activated, and a bacterial suspension is obtained; and S3, the N-(1, 3-dimethylbutyl)-N '-phenyl p-benzoquinone concentrated solution is used for strengthening Shewanella oneidensis MR-1, and hexavalent chromium is reduced. In order to solve the problem of low removal efficiency of high-concentration Cr (VI), the invention provides a method for accelerating the reduction of Cr (VI) by S.oneidensis MR-1 by using the electron shuttle characteristic of 6PPD-Q, and the method has potential environmental restoration and application values.

The invention discloses shewanella putrefaciens bacteriophage SpuP2 and application thereof. The preservation number of the shewanella putrefaciens bacteriophage SpuP2 is CCTCC (China Center For Type Culture Collection) NO: M 20251492. The method belongs to the brachycauda family. The titer of a lysate prepared based on the bacteriophage is 108-9PFU / mL, and the lysate can be stably applied under the conditions that the temperature is less than or equal to 50 DEG C and the pH is 5-10. The bacteriophage and the preparation thereof can efficiently inhibit the growth of shewanella putrefaciens, significantly inhibit the formation of a biofilm, remove a mature biofilm, and achieve a sterilization rate of 99-99.99% in aquatic products such as grass carp fillets and the like. The preparation is safe and residue-free, can replace a chemical antibacterial agent to be used for rot prevention and control in the food storage, transportation and processing processes, and has good safety and application prospects.

The invention provides a shewanella-cerium oxide nano-cluster compound, a preparation method thereof and application of the shewanella-cerium oxide nano-cluster compound in preparation of anti-radiation drugs, and belongs to the technical field of anti-radiation materials. According to the invention, a shewanella bioelectronic interface is combined with a CeO2 semiconductor nanomaterial, and when CeO2 forms an interface with shewanella and other electroactive bacteria with EET capability, metal-carrier interaction (MSI) can critically regulate and control an electronic structure of a catalytic active site. The shewanella-cerium oxide nano-cluster compound disclosed by the invention can be used for improving ion conversion between Ce < 3 + > and Ce < 4 + >, so that free radicals brought by radiation damage are reduced, and the damage of radiation to an organism is reduced. Besides, the shewanella-cerium oxide nano-cluster compound disclosed by the invention has excellent antioxidant activity, can regulate and control the intestinal microenvironment while efficiently removing various free radicals, has obvious improvement on intestinal protection after radiation irradiation, and can improve the survival rate of biological organisms.

The application discloses an iron-carbon particle, a preparation method and an iron-carbon particle containing shewanella, and relates to municipal sludge preliminary processing to form a precursor of the iron-carbon particle, acid pickling modification of the precursor of the iron-carbon particle to obtain a porous iron-carbon particle, and soaking of the iron-carbon particle in a shewanella solution to obtain the iron-carbon particle containing shewanella. Micro-electrolysis of the iron-carbon particle can promote the degradation capacity of shewanella to pollutants, and the microbial degradation of shewanella and the adsorption and degradation effect of the iron-carbon particle can further improve the removal effect of antibiotics and heavy metals. The production cost is low, the production efficiency is high, and batch production is facilitated.

The invention provides a synthetic microbial community and application thereof in degradation of trehalose and biofuel cells, and belongs to the technical field of synthetic microbiology and biological energy. A synthetic microbial community is constructed by means of synthetic biology, the synthetic microbial community comprises genetic engineering escherichia coli and shewanella, and the synthetic microbial community can realize a complete metabolic pathway from trehalose to acetic acid and an efficient electron transport chain; the method can be used for treating trehalose-rich wastewater or wastes and the like; the synthetic microbial community can be used for a microbial fuel cell, can stably generate current density and power density which are higher than those of a single strain or a natural mixed flora in the microbial fuel cell, and has good practicability.

The invention discloses construction of a shewanella hydrogengens-CdS nanoparticle hybrid system based on a hydArpt gene and an application of the shewanella hydrogengens-CdS nanoparticle hybrid system. The preparation method comprises the following steps: connecting a hydArpt gene derived from Rhodopseudomonas paclitaxel coded hydrogenase to a vector pYYDT, carrying out ribosome optimization and transformation to obtain a plasmid pYYDT-hydArpt, introducing the recombinant plasmid pYYDT-hydArpt into wild shewanella oneidensis MR-1 to obtain a recombinant shewanella engineering bacterium SERP with high hydrogen production efficiency, and carrying out self-assembly to obtain a CdS / SERP high hydrogen production activity hybrid system. The CdS / SERP hybrid system constructed by the invention endows shewanella with light capturing ability, CdS nanoparticles provide photoelectrons for engineering bacteria to promote intracellular reducing power and ATP regeneration, and the hybrid system constructed by the invention can enhance the proton reduction rate of the engineering bacteria and improve the hydrogen production efficiency.

The invention discloses a microbial electrochemical system based on aerobic extracellular electron transfer and application thereof, and belongs to the field of microbial electrochemistry, the microbial electrochemical system based on aerobic extracellular electron transfer comprises a reaction tank, the reaction tank is inoculated with flora, the flora contains shewanella genus, the dissolved oxygen concentration of the reaction tank is maintained to be greater than or equal to 1mg / L, and the dissolved oxygen concentration of the reaction tank is maintained to be greater than or equal to 1mg / L; and carrying out extracellular electron transfer on the shewanella genus. The shewanella genus is inoculated in a reaction tank and combined with the condition that the dissolved oxygen concentration is kept to be larger than or equal to 1 mg / L, the phenomenon that extracellular electron transfer can be conducted under the condition that the dissolved oxygen concentration is kept to be larger than or equal to 1 mg / L through the shewanella genus is utilized, and the shewanella genus has wide application prospects in the fields of biosensors, clean energy production and biomedicine and can also be applied to sewage treatment. And efficient electric energy recovery and COD removal are realized in sewage treatment.

The present application relates to a kind of microbial inoculant, specifically to a kind of microbial inoculant for improving subtropical secondary forest soil quality, belong to the field of biotechnology.The microbial inoculant includes A component, B component and C component;The A component includes Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus mucilaginosus;The B component includes Shewanella putrefaciens, Podospora sp. And Burkholderia sp.;The C component includes superphosphate, adhesive, soil bulking agent and water.The microbial inoculant is generated by the microbial growth-metabolism-death process of microbial residue carbon and nutrient, green, efficiently improve subtropical secondary forest soil organic matter content, phosphorus-solubilizing nitrogen-fixing, improve soil microbial community structure, promote secondary forest positive succession.

The application discloses an antagonistic bacterium of pathogenic shewanella of Apostichopus japonicus and application thereof, and belongs to the technical field of aquatic microorganisms. Gilvus japonicus The antagonistic bacterium is separated from the intestinal tract of healthy Apostichopus japonicus and is identified as Japanese Yellow Sea Oceanic Bacterium (Shewanella japonica), has no beta-hemolysis characteristic, is safe and non-toxic to Apostichopus japonicus, and can significantly antagonize the pathogenic bacterium Shewanella of Apostichopus japonicus skin rot syndrome. The application also discloses application of the antagonistic bacterium, which can be prepared into an aquatic microecological preparation for preventing and treating the skin rot syndrome of Apostichopus japonicus, and is applied through intraperitoneal injection or immersion, so that the survival rate of Apostichopus japonicus under exposure of the pathogenic bacterium is significantly improved. The strain has strong salt and alkali resistance, tolerances to various antibiotics and chemical drugs, and strong environmental adaptability, can be applied to the cultivation of Apostichopus japonicus to replace traditional antibiotics, effectively solves the problems of food safety and environmental pollution caused by the abuse of antibiotics, and has good industrial application prospect.

The application discloses a new Schumannella species with antibacterial activity and application thereof, and belongs to the technical field of microorganisms. A strain 10F1B-5-1 is identified as a potential new species of Schumannella (Schumannella), and is classified and named as Schumannella sp. The strain 10F1B-5-1 has been preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.1.16698. The Schumannella 10F1B-5-1 provided by the application has effects on gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) and gram-negative bacteria (Acinetobacter baumannii), provides a strain resource for research and development of new antibiotics, and has good research value and application potential.

The application discloses a shewanella extracellular polymer coated nano-iron material and a preparation method and application thereof, and the method comprises the following steps: after commercially available shewanella is recovered, the shewanella is expanded to a nutrient broth culture medium, and after the shewanella grows to a stable period, the bacterial liquid is washed and centrifuged, and the extracellular polymer (EPS) of the shewanella is extracted by heating. Nano-iron (nZVI) powder prepared by ball milling is added to the EPS solution, and after stirring and mixing, the EPS coated nZVI material (EPS@nZVI) is prepared. The EPS@nZVI is added to a continuous flow denitrification tank or a sequencing batch reactor for treating low carbon-nitrogen ratio sewage and is uniformly mixed and reacted, water samples are taken out, and the concentration changes of nitrate nitrogen and the toxicity of the EPS@nZVI to microorganisms are detected. In order to solve the defects of low electron utilization efficiency of nZVI in treating low carbon-nitrogen ratio wastewater and relieve the toxicity of nZVI to microorganisms, the EPS@nZVI prepared based on nZVI and shewanella EPS not only retains the strong reducibility of nZVI, but also has the potential of EPS promoting extracellular electron transfer, reduces the biological toxicity of nZVI, and has high reduction efficiency of nitrate and is easy to recycle.

Shewanella bacteria are mode electrochemically active bacteria and have bidirectional electron transfer capability, but the reverse electron transfer rate is slower. In order to solve the problem, the invention discloses a method for synchronously constructing an artificial conductive path in shewanella bacteria attachment so as to improve the electron transfer rate of a bacteria-electrode interface and the energy metabolism activity of bacteria. By utilizing the method, after dopamine is subjected to in-situ electropolymerization in the adsorption process of shewanella tamariscina PV-4, polydopamine is formed in a bacterium-electrode interface area, and the current transferred by reverse electrons is improved by 3.46 times. Due to the fact that polydopamine can serve as an artificial conductive path, the reverse electron transfer capacity of shewanella single cells is improved, and the intracellular energy metabolism level is improved through faster electron transfer.

The invention discloses a method for removing antibiotics by coupling magnetic biochar with dissimilatory iron reducing bacteria, and belongs to the field of sewage treatment. The method comprises the following steps: (1) selecting Shewanella oneidensis for enrichment culture; (2) preparing magnetic biochar; (3) mixing the bacterial strains subjected to enrichment culture in the step (1) with the magnetic biochar prepared in the step (2), so that the bacterial strains are fully adsorbed on the surface of the magnetic biochar to obtain the magnetic biochar coupled dissimilatory iron-reducing bacteria; and (4) fully contacting the magnetic charcoal coupled dissimilatory iron reducing bacteria with antibiotic wastewater to remove antibiotics in the wastewater. High density of microorganisms can be achieved, biological activity of the microorganisms can be maintained, introduction of the microorganisms effectively makes up for the single adsorption characteristic of the magnetic biochar, meanwhile, the magnetic biochar can protect the microorganisms, reduce environmental stress and ensure the stability of the microorganisms in degrading pollutants, and the removal efficiency of the pollutants is remarkably improved through cooperation of the microorganisms and the magnetic biochar.