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Saline-alkaline tolerant microbial flora and applications thereof

A salt-alkali-tolerant, microbial activity technology, applied in microorganisms, microorganism-based methods, biological sludge treatment, etc., can solve the problems of no large-scale application precedent, immature technology and process, technical bottleneck, etc., and achieve low production costs. , the effect of fast bacterial growth rate and extensive adaptability

Active Publication Date: 2015-03-11
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The domestic treatment of waste drilling mud usually adopts the method of solidification and sealing, and a few oil and gas fields adopt incineration, reinjection, and biodegradation methods, but they are currently in the stage of exploration, application, and experimentation, and the relevant technologies and processes are not mature enough.
The more advanced and environmentally friendly biodegradation technology treats waste mud, but due to technical bottlenecks, there is no precedent for large-scale application

Method used

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  • Saline-alkaline tolerant microbial flora and applications thereof
  • Saline-alkaline tolerant microbial flora and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Identification of original microbial community structure and pollutant analysis of waste drilling mud

[0016] Using the method of 16S rDNA clone library, the original microbial community structure in the waste drilling mud piled up near the port 356 well site in Dagang Oilfield, Tianjin, China was analyzed, which was used to screen the salt-alkali tolerant functional microbial community and optimize the culture conditions. The results showed that Pseudomonas sp. accounted for the highest proportion in the waste drilling mud, reaching 27.44%; Rhizobium sp. and Rhodobacter sp. also accounted for a higher proportion, respectively 20.73% and 8.54%. The proportion of Dethiobacter alkaliphilus in the community is 7.93%, which is directly related to the high sulfur content in the waste drilling mud.

[0017] The basic physical and chemical properties of the waste drilling mud were analyzed, and it was found that the pH of the waste drilling mud was 8.2-8.5, the pe...

Embodiment 2

[0018] Example 2: Screening of saline-alkali-tolerant active flora and analysis of community structure

[0019] The activity characteristics of the saline-alkali-tolerant active microbial flora depend on the particularity of its growth environment and the environmental tolerance obtained from long-term domestication. The following methods are used to screen the saline-alkaline-tolerant microbial flora.

[0020] 1. Preparation of culture medium: according to the ratio of 100g / L, weigh waste drilling mud and inoculate it into a 500mL Erlenmeyer flask prepared in advance with 200ml of crude oil inorganic salt medium containing 2% (w / v) and mix well, 180rpm, 30°C Shaking culture, the medium composition is as follows (w / v): potassium dihydrogen phosphate 0.005%, dipotassium hydrogen phosphate 0.005%, ammonium nitrate 0.005%, magnesium sulfate 0.0025%, calcium chloride 0.005%, ferrous sulfate 0.0005%, with 5% solid additives, 5% no solid phase additives, pH 7.5, low organic carbon s...

Embodiment 3

[0023] Example 3: Verification of the function of active flora by compounding single bacteria

[0024] According to the ratio of the active functional flora screened in Example 2, respectively select Alishewanella fetalis isolated from mud samples, CGMCCC No.1.8064, and Alishewanella agri isolated from soil samples, CGMCCC No.1.10668, Pseudomonas xiamenensis isolated from activated sludge samples, CGMCCC No.1.6446, Halomonas xianhensis isolated from oil-contaminated soil samples, CGMCCC No.1.6848 and isolated from high-saline soil samples Halomonas climberi, Halomonas ventosae, CGMCCC No.1.6331, cultured in LB medium to logarithmic growth phase, after which the cell concentration was adjusted according to Alishewanella: Pseudomonas: Halomonas Bacteria Halomonas=4:3:1 ratio to form the microbial flora. The addition ratio of the different species of Alishewanella and Halomonas can be arbitrary.

[0025] According to the activity detection method described in Example 2, the cru...

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Abstract

The invention provides a saline-alkaline tolerant compound active microbial flora, which is used to process waste drilling well slurry and oil-containing slurry with a high saline-alkaline content. The provided saline-alkaline tolerant active microbial flora contains shewanella mac donell, pseudomonas, and halomonas, wherein the preferable number ratio of shewanella mac donell to pseudomonas to halomonas is 4:3:1. The provided flora has a strong endurance capacity on pH and salt content, and can well adapt to the various environments of waste slurry in drilling well and soil polluted by petroleum. The additives and harmful substances in the waste slurry do not have a prominent inhibition effect on the active microbial flora. The growth speed of the flora is quick, the activity of the flora is stable, and the adaptability of the flora is strong. The production cost is low. The flora can be applied to the biodegradation of waste slurry in drilling wells, repairmen of environment destroyed by petroleum, and chemical engineering industry. The provided flora can increase the biodegradation efficiency on slurry in drilling wells and further improve the related processing technology.

Description

technical field [0001] The invention belongs to the technical field of compounding and screening of functional microbial flora, and in particular relates to a saline-alkali-resistant microbial flora and its application. Background technique [0002] Abandoned drilling mud is the mixed waste of oil, water and mud produced during the drilling process of oil and gas fields, mainly including waste drilling fluid, waste liquid, drilling cuttings and petroleum hydrocarbons. The purpose of adding drilling fluid is to achieve safe and rapid drilling. As the drilling depth increases and the difficulty increases, the additives and toxic and harmful components also increase, and the composition becomes more and more complex, mainly containing heavy metals (Cr, Hg, Cd, Pb, etc. ), petroleum hydrocarbons, bentonite, alkaline compounds, etc. are harmful to humans, animals and the environment. The waste liquid in the waste drilling mud is produced in the process of oilfield exploration an...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/00C02F11/02C12R1/01
CPCB09C1/10C02F11/02C02F2103/10C02F2303/06C12N1/20
Inventor 王兴彪黄志勇董大媛常允康
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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