363results about How to "Fast growth rate" patented technology

The invention discloses ankistrodesmus sp containing lipid as well as culture and applications of ankistrodesmus sp, wherein the ankistrodesmus strain is SS-B7, the classification name of SS-B7 is Ankistrodesmus sp, and the ankistrodesmus sp SS-B7 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 15, 2013, and has the preservation number CGMCC No.7478. The ankistrodesmus sp SS-B7 has tolerance to CO2 and NOx with high concentrations, and has high carbon sequestration efficiency, the content of total lipids in the cells of the obtained biomass accounts for 40% or above of the dry cell weight, and the ankistrodesmus sp SS-B7 can be used for producing biodiesel.

The prevent invention provides full artificial controlling culture environment a method for achieving high density intensive artificial culture of clamworm in indoors. This method is according to clamworm living characteristics to build culture pond, collocate sediment and rear with artificial diet, to make clamworm suit for culturing in indoors and without the limit of climatic environment, aquaculture production can be carried out throughout the year. To compare with existing clamworm culture mode, this method ensures high survival rate and high growth rate of clamworm seedlings. To adopt this method to culture clamworms, the survival rate reaches 70 percent to 90 percent, the weight of finished clamworm is 5 to 10 gram. The annual average yield of grub increases 2 to 3 gram, and supplying living clamworms for export the whole year.

The invention discloses a tissue culture method of ginseng adventitious root, comprising the steps of induction, amplification and successive transfer culture of ginseng callus; the induced callus is inoculated on the prepared solid culture medium and added with plant growth regulator to be cultured for 3-4 weeks under the conditions of sterility, the temperature of 23-25 DEG C and keeping in dark place; the amplified callus is inoculated on the prepared solid culture medium and added with proper plant growth regulator to be cultured for 3-5 weeks under the conditions of sterility, the temperature of 23-25 DEG C and keeping in dark place, and then the adventitious root can be formed; the formed adventitious root is picked out and inoculated on the prepared liquid culture medium and added with the plant growth regulator to be cultured for 4-5 weeks under the conditions of sterility, room temperature and sunshine. The method has higher actual significance than ginseng cell cultivation, and the growth speed of the adventitious root is much higher than that of the original root; furthermore, the production rate of active secondary metabolite of the adventitious root is high and stabler and is not influenced by natural environment such as natural disaster and the like; the tissue culture method also has the characteristics of simple technique process, high inductivity, good repeatability, rapid propagation of the adventitious root, etc.

The invention discloses a Grifola frondosa strain for producing polysaccharide with a composite raw material of rice bran and wheat bran, which belongs to the technical field of microbial application technology and food biology. The Grifola frondosa strain JSU10 is preserved in China general microbiological culture collection center (CGMCC) in October 8th, 2010 with the CGMCC No. 4179 and is identified to be Grifolasp. The invention improves the positive mutation rate of the Grifola frondosa strain for rapidly growing and producing Grifola frondosa polysaccharide on a composite culture medium of rice bran and wheat bran by composite mutagenesis of ultraviolet rays and microwave to obtain a high-yield strain; the strain and the original strain are respectively a liquid fermentation rice bran and wheat bran composite culture medium; and hypha dry weight and polysaccharide of the mutated strain are respectively improved by 39.24 percent and 42.58 percent compared with the original strain.

The invention belongs to the fields of microbe application technologies and food biotechnologies, and discloses a strain used for fermenting rice bran and wheat bran extracts for producing grifolan. The grifola frondosa strain is collected in China Center for Type Culture Collection (CCTCC) in Wuhan University in Wuhan, China on Aprial 7th, 2011, and has a strain collection number of CCTCC No: M2011113. The name of the strain is Grifolasp. JSU10-2. According to the invention, through protoplast laser mutation, the strain with high yield of mycelium polysaccharide produced from cheap raw materials is obtained. When the strain and an original strain are respectively used in liquid fermentation of a rice bran and wheat bran composite culture medium, the dry weight and polysaccharide of the mutant strain are respectively increased by 31.7% and 32.6% compared with those of the original strain.

The present invention relates to a method for obtaining a recombinant yeast of Saccharomyces cerevisiae, which ferments lignocellulose raw materials to ethanol, including introducing DNA into a yeast so as to cause the yeast to have introduced genes encoding xylose reductase, xylitol dehydrogenase and xylulokinase.

The invention breaks through the restriction of the traditional autotrophic nitrification and anoxic denitrifying metabolism principle, and discloses a method for removing carbon and nitrogen pollutants in waste water in one step according to physiological feature and metabolism principle of heterotrophic nitrification-aerobic denitrification bacteria. In a single biochemical reactor, mixed solution of the heterotrophic nitrification-aerobic denitrification bacteria and active slurry in a certain proportion is inoculated, after a certain period of aeration is performed, nitrogen and organic substances are removed at the same time, and the efficiency of denitrification and carbon removal is high. By using the method, a traditional biochemical treatment system can be updated and transformedwithout constructing a new building based on the traditional biochemical treatment system, and the operation is convenient and fast. The method overcomes the bottleneck problems that the nitrobacteria propagate slowly, the nitrification and denitrification reactions are performed in sections, and the like.

The invention relates to a method for rapidly cultivating chlorella and belongs to the technical field of biology. The cultivation method comprises the following steps: preparing a standard BG-11 culture medium to serve as a basal culture medium of chlorella by taking fresh running water as a solvent; adding a certain amount of organic carbon source and NaHCO3 into the standard culture medium, so that the final concentration is respectively 0.5-1.0g / L and 0.2-0.5g / L; putting the culture medium into a culture vessel, inoculating 10-20 percent of chlorella solution, sealing the opening, and culturing the chlorella solution in an environment with the illumination intensity of 3000-4000Lx, the light-dark ratio of 12h:12h and the temperature of 22-28 DEG C. According to the method, the cell density of the chlorella can reach a high level in a short cultivating cycle and is increased by about six times compared with that cultivated by using the basal culture medium. Meanwhile, the method has the advantages that the process is easy to operate, the culture medium is not required to be sterilized, the pH value of the culture medium is not required to be regulated, and the method can be widely applied to cultivating chlorella in industrial production.

The invention relates the construct method for transgenosis rhinestone algae bioreactor. The method comprises the following steps: using the rhinestone algae as transgenosis receptor biology, highly effective expressing the carrier construct by constructing the exogenesis gene containing screening mark expression frame, leading the destination genes in rhinestone algae with bead milling method, electric punching method and other methods, integrating them into nucleus genome or chloroplast genome, and adopting a series of gene expression regulate and control technology to construct the transgenosis rhinestone algae bioreactor. The transgenosis rhinestone algae bioreactor possesses the characters of fast growth rate, short breeding cycle, easy segregation, photoautotroph and large scale culture, fast and cheap producing medicinal protein, industrial raw materials, and feeding vaccination, and possessing the bioactivity secondary metabolite.

The invention relates to a licorice rapid propagation seedling tissue culture method. The method comprises steps of licorice aseptic seed germination culture, licorice aseptic seedling culture, licorice rapid propagation seedling induction and unrooted rapid propagation seedling rooting culture, the steps are respectively carried out in an MS solid medium containing 20-30g / L of sucrose under aseptic 24-26DEG C conditions, mediums for the rapid propagation seedling induction and the rapid propagation seedling rooting are added with a plant growth conditioning agent, and the pH of the MS solid medium before disinfection is adjusted to 5.9-6.0. The tissue culture method which is utilized in the invention to culture the licorice rapid propagation seedling has more practical meanings than cell culture and hairy root culture, and allows the growth rate to be rapid and the propagation multiple to be high to 6 times. The method of the invention, which allows the nursery stock growth to be stable and the culture to be uninfluenced by natural environments of natural disasters and the like, has the characteristics of simple process, high inductivity, good repeatability, high rapid propagation seedling increment multiple and the like.

The invention discloses a method for preparing a zirconium diboride coating and relates to a method for preparing a ceramic coating. The invention aims to solve the problems that a gas circuit needs to be subjected to thermal insulation treatment and the requirement of a gas flowmeter is high by adopting a method for heating ZrCl4 to be higher than a sublimation temperature and introducing the ZrCl4 into a reaction chamber through the flowmeter in the process of preparing ZrB2 through a conventional chemical vapor deposition method. The preparation method comprises the following steps: by using a double-temperature-area heating mode, by taking ZrCl4, BCl3 and H2 as source gases, by taking Ar gas or N2 as carrier gas and protective gas, preparing the zirconium diboride coating by adopting the chemical vapor deposition method. According to the method in the invention, the gas circuit needs no special thermal insulation treatment, and the flow of the ZrCl4 can be controlled through a temperature control method. The method can be used for preparing zirconium diboride coating.

The present invention discloses the process of culturing adventitious root tissue of licorice, and belongs to the tissue culture of licorice as one kind of Chinese medicinal materials. The process of the present invention includes soaking licorice seed in solution with ethanol in 70-75 vol% and sodium hypochlorite in 2 vol% to sterilize and planting in MS culture medium to induce bacteria-free bud; inoculating the cut cotyledon and bud in MS culture medium containing different plant growth regulator to induce callus; transferring the callus growing for 20 days in MS culture medium with indolebutyric acid and kinin to induce adventitious root; and finally transferring the adventitious root to liquid culture medium containing the same plant growth regulator for fast growth. The present invention has simple process, high inducing rate, fast adventitious root proliferating speed and other features.

The invention provides a recombinant DNA sequence, hansenula polymorpha, a preparation method for hepatitis B surface antigen, and a hepatitis B vaccine. The recombinant DNA sequence is obtained by codon optimization of coding genes of the hepatitis B virus surface antigen according to codon usage frequency of the hansenula polymorpha. The invention also provides the hansenula polymorpha comprising the recombinant DNA sequence, a method for preparing adr sub-type hepatitis B surface antigen by using the recombinant DNA sequence, and the hepatitis B vaccine. The adr sub-type hepatitis B surface antigen has high expression level of the recombinant DNA sequence. The recombinant hansenula polymorpha is fast in growth speed, has high HBsAg yield, can be fermented in high cell density by using a cheap chemically synthetic medium, has low fermentation contamination rate and is beneficial to large-scale production; and HBsAg adr vaccine provided by the invention has high trend of Th1 and Th2 type cellullar immunologic response.

The invention relates to the method of growth of gallium nitride film in high quality by the technique of extension in the lateral direction. GaN crystal seed layer is grown by use of the MOCVD, MBE or other method. The SiO2, Si3N4W etc. films are deposited on the GaN crystal seed layer with sertain graph being etched by etching method. The direction of the parallel strip opening is along the direction of GaN [1100]. The width of the mask area is 2-20 microns. The width of the GaN window area is 0.2-20 microns. Then the MOCVD or HVPE method is accepted to grow GaN in epitaxial growth till the mask layer is overspreader by the GaN. Continuing growth obtains the gallium nitride film with low dislocation density. Since HVPE providing quick growth rare, to the HVPE thick film extension can obtain GaN film even lower dislocation density.

The invention discloses Desmodesmus sp. rich in oil, and culture and application thereof. The Desmodesmus sp. is MH-04 preserved in China General Microbiological Culture Collection Center on April 24, 2015, with an accession number of CGMCC No. 10764. The Desmodesmus sp. can tolerate high-concentration CO2 and SO2, is capable of realizing photoautotrophic growth in virtue of exhaust gas or flue gas containing CO2 and SO2 to acquire biomass and has a high carbon sequestration rate; and the obtained biomass is rich in oil and can be used for production of biodiesel.

A sieve method of heterotrophic nitrifying bacteria belongs to sewage treatment technical sphere. The invention is to carry out heterotrophic nitrifying phenomenon from the biology angle by screening heterotrophic nitrifying bacteria and the technology is the condition precedent to carry out short-range self-synchronous nitration and denitrification. The invention is proceeded by the following steps: 1. enriched culturing of microorganism; 2. picking out single colony from beef cream protease leather solid medium and seeding them in ammonifying culture medium to culture and purify; 3. picking out single colony from ammonifying culture medium solid medium and seeding them in nitrosation culture medium to culture and purify to obtain heterotrophic nitrifying bacteria. The heterotrophic nitrifying bacteria of this invention not only can grow and breed in culture medium using ammonia nitrogen as unique nitrogen source but also can normally grow and breed in culture medium using nitrite as unique nitrogen source, which has stronger adaptive capacity for environment and has bigger development potential in actual engineering application.

The invention provides a feed for sea cucumbers. The feed comprises the following components: 50 to 90 weight parts of seaweed powder, 2 to 30 weight parts of zeolite powder and 15 to 40 weight parts of protein powder, wherein the zeolite is clinoptilolite or / and mordenite. The feed for the sea cucumbers is prepared by a method comprising: activating the zeolite, crushing the activated zeolite powder and mixing the activated zeolite powder and other components, and sieving the mixture; or adding water to mix the zeolite powder and other components to form the feed with certain thickness. The feed for the sea cucumbers is the nutritive green feed, which has the advantage of avoiding pollution, high oxygen consumption, drug resistance, residue and toxic and side effects. When the feed is used, the utilization rate high; the nutrient components are hardly lost; and water quality is not deteriorated; and when used as the bait or feed for sea cucumbers, the feed has the advantages that: the survival rate of the sea cucumber juveniles is high; weight incensement of the sea cucumber juveniles is high; and the like.

The invention relates to the biotechnical field, particularly relates to the technical field of animal biological product engineering and more particularly relates to serum-free culture media and application thereof as well as a culture method of a classical swine fever virus by using the culture media. According to the preparation method of the serum-free culture media, with an MEM (minimum essential medium) as a base culture medium, based on research on growth and multiplication characteristics of a swine testis (ST) cell and the classical swine fever virus, by adding different components into the base culture medium, a series of swine testis cell serum-free culture media as well as classical swine fever virus multiplication serum-free culture media for different growth stages are obtained respectively; therefore, possible pollution risks of BVDV (bovine viral diarrhea virus) and an antibody thereof are avoided, cell adherence growth of the ST cell can be realized at the same time; and moreover, the ST cell can grow quickly, has an intact cell side, is full in individual and is capable of being sub-cultured for a long time.

The invention discloses a biological purification agent applied to sewage of livestock and poultry farms and a preparation method thereof. The preparation method comprises the following steps: firstlyadding a compound microorganism bacterium agent into an immobilized carrier formed by three carriers including sodium alginate zeolite, sodium alginate and polyvinyl alcohol to obtain a bacterium solution, wherein the mass ratio of the immobilized carrier to the compound microorganism bacterium agent is (200-300) to 1, the compound microorganism bacterium agent contains rhodospirillum, nitrifyingbacteria and bacillus subtilis according to a volume ratio of 1 to 1 to 1, and the number of effective living bacteria in per gram of the compound microorganism bacterium agent is greater than or equal to 109; then dropping the bacterium solution into a boric acid saturated solution with 4-8% of CaCl2 to obtain globular immobilized compound microorganisms; finally enabling the immobilized compound microorganisms to stand and cure under the condition of 4 DEG C, carrying out immobilized crosslinking for 24 hours to obtain immobilized microorganism globules, washing the immobilized microorganism globules with deionized water for at least three times to obtain the biological purification agent. The preparation method is simple and is low in cost; the prepared purification agent has strong stability, and high microorganism activity, is environmentally friendly, and is free of secondary pollution.

The invention discloses a mutagenic screening method of strain mortierella alpina for generating arachidonic acid. After analyzing a microbe glycolytic pathway, a tricarboxylic acid transferring system, a fat acid synthesizing pathway and other related pathways in the process of producing arachidonic acid (AA) by fermenting the mortierella alpina, iodoacetic acid and acetylsalicylic acid are used as screening agents, and by a plurality of steps of activation of seeds, culture of the seeds, pretreatment of a seed solution, mutation of the treated seed solution, primary screening, secondary screening, third screening and the like, a strain with high growth rate of the arachidonic acid, short fermenting period and large increase range of the yield of the arachidonic acid can be obtained. The method of the invention is an ideal rational strain selective breeding technology and enjoys large economic benefits and social benefits and good industrialization prospects.

The invention relates to a Mucor circinelloides strain with high grease yield and application thereof. The invention discloses a Mucor circinelloides strain WJ11 and application thereof (a method for producing grease by using the strain). The method comprises the following steps: activating the strain to obtain an activated strain solution, carrying out seed culture under sterile conditions to obtain a seed culture solution, carrying out fermentation culture to obtain a fermentation culture solution, filtering, carrying out freeze-drying to constant weight to obtain a grease-containing Mucor circinelloides WJ11 dry strain, grinding, and extracting grease by a chloroform-methanol process. The Mucor circinelloides strain WJ11 has the advantages of high growth rate and high fatty acid content (up to 30-40% of biomass); the fatty acid content is 2-3 times of the control strain; and the biochemical analysis result shows that the Mucor circinelloides strain WJ11 has obviously higher enzyme activity related to fatty acid accumulation than the control group. The Mucor circinelloides strain has very wide application prospects when being used in microbial grease-producing industry and microbial grease accumulation analysis.

The invention relates to a high-early-strength filling material for a coal mine goaf. The high-early-strength filling material is prepared from the following components in parts by weight: 10-20 parts of quick-hardening sulphate aluminate cement, 40-80 parts of coal ash, 150-200 parts of coal gangue particles, 1-3 parts of a content-available superplasticizer and 0.1-1 part of a thickener. The high-early-strength filling material is used for filling the coal mine goaf and is remotely pumped, and is high in strength increase speed and filling efficiency, low in filling cost, safe and environmentally friendly. An experiment shows that the early strength of the high-early-strength filling material is high and can reach 10.0MPa, and the compressive strength can reach 18.0MPa in the 28th day.

The invention discloses a preparation method of super-lubricity amorphous carbon film with a nano-structure. The film is prepared by adoption of a radio frequency magnetron sputtering physical vapor deposition technology, and is silicon- aluminum- co-doped amorphous carbon film having super-lubricity performance in a high vacuum environment. The film has the nano netlike and fullerene-like compound nano-structure, and has excellent elastic recovery performance. In addition, even the nano hardness of the film is low and the film belongs to soft film, the film shows excellent frictional wear performance in the vacuum environment, the lowest friction coefficient is 0.001 and the wear rate is reduced to the order of magnitudes of 10<-19> m3 / N*m. The film is uniform and compact, and firmly combines metal based materials. The film is a solid lubrication and sealing material, and has a good application prospect with respect to various mechanical motion parts in the vacuum environment.

The invention relates to a crucibleless growing method for a magneto-optical crystal of rare earth ferrite, which belongs to single crystal growth filed. The invention has the characteristics that oxide is used for resource material and preburned according to proportion, a material rod is obtained through static pressure molding and high temperature sintering, and then the material rod is set into a crucibleless crystal growth furnace and is grown in air condition. The invention has the characteristics that a crucibleless technology is used for growing RFeO<3>crystal, and none complete fusion is needed, and pollution for the complete fusion and a copple in the growing process are utmostly decreased, impurity defect in a magneto-optical crystal is sharply decreased, and crystal quality is progressed.

The invention relates to monoraphidium sp. SHJ-02, with the classification name of Monoraphidium sp. preserved in CGMCC (China General Microbiological Culture Collection Center) of CCCCM (China Committee for Culture Collection of Microorganisms) on April 24, 2015, with the preservation number being CGMCC N0.10763. The strain can grow efficiently under the autotrophy condition to obtain oil-rich biomass; besides, the strain has high environment adaptability, can still grow more quickly at the low temperature, has high oil content, has the cell total lipid content being 40% of the dry cell weight of cells or above and can be applied to production of biodiesel.

The invention discloses a method for reinforcing an adsorption-biodegradation (AB) denitrification technology by use of heterotrophic nitrification-aerobic denitrification bacteria and belongs to the technical field of water treatment. The method comprises the following specific steps: when the COD (chemical oxygen demand) / N of sewage is more than 8, the sewage passes through a section A and a section B in turn, feeding the effluent of the section A into the section B and inoculating heterotrophic nitrification-aerobic denitrification bacteria into the activated sludge of the section B; and when the COD / N of the sewage is no more than 8, feeding the sewage into the section A and the section B respectively, independently operating the section A and the section B and inoculating the heterotrophic nitrification-aerobic denitrification bacteria into the activated sludge of the section A and the activated sludge of the section B. By adopting the method, the functions of carbon removal and denitrification are realized under the combined action of the section A and the section B in an AB process system and the denitrification effect and efficiency of sewage treatment can be increased.

The invention provides a zinc-rich saccharomyces cerevisiae Z1.4CCTCC M 205126. The zinc-rich saccharomyces cerevisiae has stable zinc resistance, fast growing speed and high utility ratio of zinc and contains zinc as high as 80,000mg / kg, wherein the content of biological zinc can reach 90 percent.

The invention relates to a thin film and a preparation method therefor, and provides an electrochromic multi-layered transparent conductive thin film and a preparation method therefor. The multi-layered transparent conductive thin film comprises a substrate, a first WO3 thin film layer, an Ag conductive layer, a W buffer layer and a second WO3 thin film arranged from upper to lower in sequence; the preparation method comprises the steps of preparing the first WO3 thin film layer on the substrate through a reactive sputtering method; then preparing the Ag conductive layer and the W buffer layer on the surface of the first WO3 thin film layer through a direct current sputtering method and a magnetron sputtering method separately; and finally preparing the second WO3 thin film layer on the surface of the W film layer through the reactive sputtering method. The multi-layered transparent conductive thin film structure and the preparation process are optimized to prepare the electrochromic multi-layered transparent conductive thin film through the sputtering method; and the thin film has the advantages of high transmittance within a visible light range, high conductivity, large adjustment range for the visible light transmittance, high coloring efficiency, high cycling stability, environment friendliness and the like.

The invention discloses a shallow trench isolation manufacturing method. The technical procedures for forming the shallow trench isolation and an active region on a silicon substrate comprise that non-crystallizing ions are injected in the position at the edge of the active region so as to allow the crystalline silicon on the surface of the active region at the injection position to be subjected to non-crystallizing processing. According to the method provided by the invention, during the following growth process of a gate oxide layer, the growth speed at the border edge is faster than that in the active region, the thinning effect of the edge thickness of the gate oxide layer is reduced, gate breakdown voltage and gate breakdown service life of a device is improved, and accordingly, the reliability of the device is improved.

The invention relates to plant tissue culture and discloses a tissue culture method for Vaccinium bracteatum Thunb, belonging to the field of plant cultivation. The method comprises the following four steps: explant sterilization; induction culture; propagation culture; and rooting culture. The method has the advantages of a high propagation rate, a fast growth rate and high hereditary stability, overcomes the problem of difficult breeding of Vaccinium bracteatum Thunb, can realize large-scale production and industrial large-scale supply of finished seedlings, protects excellent wild plant resources and brings about good economic values.