The invention discloses a tissue culture method for inducing and proliferating bovine vigorous hairy roots. In the method, the cotyledons of the boulders are used as explants to induce hairy roots, and the method includes explant sterilization, Agrobacterium activation culture, hairy root induction culture, proliferation culture and the like. Advantages of the present invention: (1) adopt the cotyledons of Bovine vigor to transform as explants; (2) Establish the pre-culture time, bacterium concentration, infection time and co-cultivation time suitable for the induction of bovine vigor hairy roots; (3 ) determined the best bacteriostatic agent for explant bacteriostatic culture; (4) determined the best pre-cultivation, co-cultivation, bacteriostatic culture and subculture medium; (5) the whole set of method operation provided by the present invention It is simple, low in production cost, high in induction success rate, and fast in proliferation, and can sustainably obtain the hairy root of the plant, which can be used for large-scale cultivation of the hairy root of the plant to extract the main chemical components such as the polysaccharide of the plant, and has a good application prospect .