41 results about "Hairy root culture" patented technology

A method for the genetic transformation of transgenic Stevia rebaudiana mediated by Agrobacterium rhizogenes, in which the stem tip explants of Stevia rebaudiana after precultivation are immersed in the Agrobacterium rhizogenes bacterium liquid carrying the pRI 101-AN DNA plasmid of the gene of interest, After infection, co-cultivation and sterilization treatment, the hairy roots were cultured on the selection medium containing kanamycin, and induced the formation of kanamycin-resistant hairy roots on the explants, and induced the formation of hairy roots The callus differentiates into adventitious buds, and the adventitious buds continue to grow to form test-tube plantlets, and PCR amplification method is used to identify transgenic plants carrying the target gene. By adopting the technical scheme provided by the invention, the target gene can be introduced into Stevia rebaudiana, and compared with the genetic transformation of chemical methods such as polyethylene glycol, the link of isolation and cultivation from protoplasts is reduced; and the genetic transformation of physical methods such as gene guns In contrast, it does not require expensive instruments and equipment, and the operation technique is simple.

The invention provides an induced proliferation method for glehnia littoralis hairy roots. The method comprises the following steps: obtaining glehnia littoralis aseptic seedlings; culturing agrobacterium rhizogenes for infection; performing co-culture on the transformed explant; and inducing hairy root generation and hairy root culture proliferation. The formation of glehnia littoralis hairy roots is induced by utilizing the agrobacterium rhizogenes infection, an in-vitro hairy root culture system is established, the content of medicinal component coumarin is analyzed and measured, the content of medicinal component coumarin is contrasted with the content of coumarin in adventitious root and artificially cultured glehnia littoralis roots, the content of coumarin in the hairy roots is the highest, and the glehnia littoralis hairy roots has the advantages of fast growth, short period, relatively high yield of the secondary metabolite and the like and can be used for industrial production of glehnia littoralis coumarin; and moreover, a novel path is provided for industrial production of the secondary metabolite of the glehnia littoralis by utilizing the hairy roots in the future, the threatened plant is protected, and the ecological balance is maintained.

The invention relates to a method for increasing the content of flavonoids in Scutellaria baicalensis, which belongs to the field of biotechnology; adopting biochemical engineering technology, using rare earth praseodymium to induce the hairy roots of Scutellaria baicalensis to increase the content of flavonoids, which specifically includes the following steps: (1) sticking Suspension culture of hairy roots of Scutellaria baicalensis: Weigh about 100 mg of fresh hairy roots of Scutellaria baicalensis, place them in a large-scale culture container, and then add 1 / 2 MS0 medium with a volume fraction of 80% for suspension culture; (2) Pr Treatment of elicitors on the hairy root culture of Scutellaria baicalensis: add 25mmol / L Pr3+ solution to the culture medium and place it at a constant temperature of 25°C±1.0°C for 7 days; (3) Extraction and detection of flavonoids ; The present invention uses rare earth praseodymium to improve the medium, which can efficiently produce a new culture system of baicalin flavones. Compared with the 1 / 2MS0 control medium, the production of flavones increases by 4.502 times; A new high-quality sustainable drug source.