A kind of tissue culture method for the induction and proliferation of bovine vigorous hairy roots

Abstract

The invention discloses a tissue culture method for inducing and proliferating bovine vigorous hairy roots. In the method, the cotyledons of the boulders are used as explants to induce hairy roots, and the method includes explant sterilization, Agrobacterium activation culture, hairy root induction culture, proliferation culture and the like. Advantages of the present invention: (1) adopt the cotyledons of Bovine vigor to transform as explants; (2) Establish the pre-culture time, bacterium concentration, infection time and co-cultivation time suitable for the induction of bovine vigor hairy roots; (3 ) determined the best bacteriostatic agent for explant bacteriostatic culture; (4) determined the best pre-cultivation, co-cultivation, bacteriostatic culture and subculture medium; (5) the whole set of method operation provided by the present invention It is simple, low in production cost, high in induction success rate, and fast in proliferation, and can sustainably obtain the hairy root of the plant, which can be used for large-scale cultivation of the hairy root of the plant to extract the main chemical components such as the polysaccharide of the plant, and has a good application prospect .

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, in particular to a tissue culture method for inducing and proliferating bovine hairy roots. Background technique [0002] Niu Dali is a plant of the genus Millettia speciosa Champ. in the leguminous family, also known as Dali potato and mountain lotus root. ) local medicinal materials. The root of Niu Dali is used as medicine, which is sweet and flat. Return lung, kidney channel. It has the effect of tonifying deficiency and nourishing the lungs, strengthening tendons and activating collaterals. It has been clinically proven to have good curative effect on chronic diseases such as lumbar muscle strain, rheumatoid arthritis, lung deficiency cough, tuberculosis, chronic bronchitis, and chronic hepatitis. The folks of the Zhuang people are often used for the treatment of lumbago and leg pain, fever (rheumatic bone pain), tuberculosis, chronic hepatitis, and chronic gastritis. [0003] M...

AI Technical Summary

Problems solved by technology

In order to solve the contradiction between supply and demand, people try to expand the drug source by artificial cultivation. Some successful research reports have been found so far, but there are still many problems restricting its large-scale production. It is very unstable, the survival rate of cutting seedlings is low, and it is very difficult for tissue culture seedlings to take root, etc.

Moreover, the growth cycle of cattle vigorously is long (generally 3-4 years before the production is formed), and it takes a long time to breed or raise seedlings by conventional methods. Due to the small reproduction coefficient and large seed consumption, the development speed is very slow and the production cost increases. Moreover, serious degradation due to damage by diseases and insect pests has seriously affected the yield and quality of medicinal materials.