16 results about "Cotyledon plant" patented technology

The invention belongs to the technical field of gene engineering, and discloses application of a Camellia oleifera CoSPX-MFS3 gene in improvement of phosphorus absorption of plants, and the nucleotide sequence of the Camellia oleifera CoSPX-MFS3 gene is as shown in SEQ ID NO.1. The invention further discloses a preparation method of the Camellia oleifera CoSPX-MFS3 gene. The plant is any one of dicotyledonous plants or monocotyledonous plants. The dicotyledonous plant is any one of arabidopsis thaliana, tobacco or tomato; the monocotyledonous plant is any one of rice, wheat or corn. The low-phosphorus stress can induce the expression of the gene, and the abundance in roots and fruits is the highest. The CoSPX-MFS3 is constructed to an overexpression vector pBI121, and the overexpression vector pBI121 is converted into the silver gray poplar, so that a positive strain is obtained. The invention discloses the application of the Camellia oleifera CoSPX-MFS3 gene in improvement of phosphorus absorption of the plant for the first time, and experiments carried out in the Populus arvensis show that the Populus arvensis plant over-expressed with the CoSPX-MFS3 gene can significantly improve the phosphorus absorption capacity of the plant.

This disclosure concerns the use of endogenous plant RNAi machinery to preferentially or specifically reduce transgene expression. In some embodiments, the disclosure concerns specific reduction of transgene expression in seed tissues of a dicot plant.

This invention discloses a gene for inducing parthenogenesis in dicotyledonous plants. ZbRWP Its applications, specifically, the parthenogenesis-inducing gene in dicotyledonous plants. ZbRWP This invention is the first to discover and verify its application in cultivating haploid or haploid induction lines of dicotyledonous plants or improving haploid induction ability. RWP This gene family possesses parthenogenetic haploid induction function in dicotyledonous plants, filling a gap in the list of parthenogenetic haploid induction genes in dicotyledonous plants. This invention lays an important foundation for broadening the application of haploid breeding technology in dicotyledonous plants and elucidating the biological mechanism of parthenogenetic haploid formation. Given the widespread use of haploid breeding technology in the current breeding industry, this invention has broad application prospects in the field of bio-breeding.

The invention provides a monocotyledonous plant gene editing vector and a gene editing method and application thereof, and particularly provides an optimized nucleotide sequence for coding CasWM protein, a nucleic acid construct for evaluating CasWM expression quantity or editing efficiency thereof, a nucleic acid construct for gene editing, a nucleic acid construct for gene editing and a nucleic acid construct for gene editing. The invention also provides a reagent combination for gene editing, and the optimized CasWM nucleotide sequence obtained by screening is introduced into plant cells, so that very high editing efficiency is obtained.

The application discloses a dicotyledon parthenogenesis induction gene BoRWP and application thereof, in particular a dicotyledon parthenogenesis induction gene BoRWP application in cultivating dicotyledon haploid or haploid induction line or improving haploid induction capacity, the application firstly discovers and verifies that RWP a gene family has parthenogenesis haploid induction function in dicotyledon, and fills the blank of dicotyledon parthenogenesis haploid induction gene. The application lays an important foundation for widening application of haploid breeding technology on dicotyledon and revealing biological mechanism of parthenogenesis haploid generation. In view of the wide use of haploid breeding technology in the current breeding industry, the application has wide application space and prospect in the field of biological breeding.

The present invention provides methods for increasing the efficiency of forming doubled haploid plants by increasing the number of opportunities to form doubled haploid seeds by treating monocotyledonous plants with plant growth regulators. In certain embodiments, a corn plant comprising a plurality of co-dominant ears is prepared. Plants comprising the potential to produce increased generations after doubled haploids are also provided.

The invention discloses a dicotyledonous parthenogenesis induction gene RWP and application thereof, and particularly relates to application of the dicotyledonous parthenogenesis induction gene RWP in cultivation of dicotyledonous haploid or haploid induction lines or improvement of haploid induction capability. The invention discovers and verifies that the RWP gene family has the parthenogenesis haploid induction function in the dicotyledon for the first time, and fills the blank of the dicotyledon parthenogenesis haploid induction gene. The invention lays an important foundation for widening the application of haploid breeding technology on dicotyledonous plants and revealing the biological mechanism of parthenogenesis haploid production. In view of the universality of haploid breeding technology utilization in the current breeding industry, the invention has wide application space and prospect in the field of biological breeding.

Monocot plants heterozygous for centromeric histone 3 (CenH3) and optionally expressing gene editing constructs, for use in inducing haploids of a monocot target plant and optionally pass-through gene editing are provided. The monocot haploid inducer plants are typically composed of diploid plant cells having only one allele encoding a functional CENH3 protein. The diploid plant cells can also include, for example, one CenH3 allele encoding non-functional CENH3 protein. In some embodiments, the allele encoding non-functional CENH3 protein is a frameshift mutation, protein null allele, an RNA null allele, or a combination thereof. The monocot haploid inducer plant can also include gene editing machinery, such as a site-directed nuclease and optionally a guide RNA stably expressed by cells of the monocot plant. Methods of inducing formation of a target haploid monocot plant while optionally simultaneously modifying the target monocot plant's genome are also provided.

Apparatus(es), system(s), and method(s) provide for automated separation / extraction and collection of embryonic axes from dicot seeds. Such apparatus(es), system(s), and method(s) can include a rotating suspension of a combination of whole dicot seeds and liquid, wherein the whole dicot seeds are comminuted to substantially separate / extract the embryonic axes. The apparatus(es), system(s), and method(s) can further include through-pores wherein the separated / extracted embryonic axes are generally passed through the through-pores for collection. Embryonic axes separated from seeds can be used for genetic research and experimentation including, but not limited to, biolistic transformation(s), biolistic gene delivery, agrobacterial transformation(s), and / or gene editing.

The disclosure provides novel methods for improving the regeneration throughput and / or plugging frequency of monocot embryo explants by flotation and selective collection of viable and regenerable explants. Genetic modification or transformation of monocot plants may include preparation of seed excised embryo explants, rehydration, inoculation and co-culture, bud induction, extended bud induction, and regeneration of genetically modified plants or plant parts. To improve regeneration and plugging frequency, cultured monocot seed excised embryo explants following the bud induction or extended bud induction step are placed in a flotation medium to separate the explants into top and bottom layers or fractions, such that the top fraction can be selectively collected and advanced to regeneration media. The top fraction is shown to have a higher or similar plugging frequency than the bottom fraction and thus the flotation step reduces costs and improves transformation throughput by removing unproductive explants in the bottom fraction having a much lower regeneration and plugging frequency.

This invention discloses a gene for inducing parthenogenesis in dicotyledonous plants. RWP Its applications, specifically, the parthenogenesis-inducing gene in dicotyledonous plants. RWP This invention is the first to discover and verify its application in cultivating haploid or haploid induction lines of dicotyledonous plants or improving haploid induction ability. RWP This gene family possesses parthenogenetic haploid induction function in dicotyledonous plants, filling a gap in the list of parthenogenetic haploid induction genes in dicotyledonous plants. This invention lays an important foundation for broadening the application of haploid breeding technology in dicotyledonous plants and elucidating the biological mechanism of parthenogenetic haploid formation. Given the widespread use of haploid breeding technology in the current breeding industry, this invention has broad application prospects in the field of bio-breeding.

The invention discloses a non-embryonic stage grafting method of monocotyledonous plants and application of grafted bodies of the monocotyledonous plants, and aims to solve the technical problems that the existing monocotyledonous plant embryonic stage grafting technology is extremely short in operation window period, strict in environmental requirement and incapable of being applied in a large scale. The key technical means of the method is that a stock and a scion which are in a non-embryonic development stage (such as a one-leaf one-core stage) are selected, notches are formed in the middle stem parts of the stock and the scion and are in butt joint, and callus formation and vascular connection are promoted through specific fixing and moisturizing treatment. According to the method, the effective grafting time is remarkably prolonged, the dependence on a sterile environment is reduced, the grafting survival rate and the operation feasibility are greatly improved, and an effective means is provided for field large-scale grafting and physiological research of monocotyledonous plants.

The invention relates to the technical field of chemical pesticides, and discloses an application of a thiophene-2-formamide compound as a herbicide, the molecular weight of the compound is 317.44 g / mol, the compound is synthesized from 5-(3-hydroxy-3-methylbutyl-1-alkyn-1-yl)-2-thiophenecarboxylic acid and 2-thiophenemethylamine, the growth and development of dicotyledonous plant seeds and roots can be inhibited, and the growth and development of the dicotyledonous plant roots can be inhibited. The growth and development inhibition concentration on dicotyledonous plant roots is greater than or equal to 50 mu M, and the growth and development inhibition concentration on dicotyledonous plant seeds is greater than or equal to 10 mu M; when the herbicide is used for preventing and removing farmland weeds, the spraying concentration is only 1mM, the action concentration is lower than the concentration of most known herbicides in the market, and the herbicide has remarkable applicability to the field of agricultural weeding.

A dicot plant cell, plant tissue or plant having a reduced expression of a wild type protein having at least 75% sequence identity with SEQ ID NO. 1 is disclosed. Also disclosed is a plant cell, plant tissue or plant comprising a modified protein and / or a truncated protein having at least about 75% sequence identity when calculated over the positions corresponding to positions 1-66 and positions 78-308 of SEQ ID NO: 1. The modified protein increases the resistance to Geminiviridae. Nucleic acid constructs encoding the modified or truncated protein are also provided. Lastly, methods for generating a plant cell, plant tissue or plant comprising the modified protein that increases the resistance to Geminiviridae are disclosed.