652 results about "Cotyledon plant" patented technology

The present invention is related to a process based on a source-sink principle, for producing products of interest from crushed or uncrushed germinating dicot seeds comprising an expression system, which is induced or can be induced during germination. The product is either a seed derived composition comprising one or more gene products. Alternatively, it is a product of interest obtained by placing the composition in contact with a substrate, containing a substance capable of being transformed by the seed derived composition as such, dried or in down-stream processed form.

Methods of increasing nitrogen utilization efficiency in monocot plants through genetic modification to increase the levels of alanine aminotransferase expression and plants produced there from are described. In particular, methods for increasing the biomass and yield of transgenic monocot plants grown under nitrogen limiting conditions compared to non-transgenic plants are described. In this way, monocot plants may be produced that maintain a desired yield while reducing the need for high levels of nitrogen application.

This invention relates to a soy protein containing food product comprising; (A) a fibrous material containing soy protein and soy cotyledon fiber, wherein said soy cotyledon fiber is present in the fibrous material in an amount of from 1% to 8%, by weight on a moisture free basis; (B) a humectant comprising (i) a colorant and at least one of (ii) a flavoring agent, (iii) a triglyceride oil, (iv) a food grade acid or acidic salt, (v) a food grade base or basic salt, or (vi) a food grade emulsion; and (C) water. In another embodiment, the invention is directed to a process for preparing a soy protein containing food product.

The invention relates to a method using the cotyledon node of soybeans as explant to perform soybean organogenesis and plant regeneration, belonging to the technical field of agricultural production. The invention is characterized in that based on the traditional tissue culture, the cotyledon node of soybeans is used as explant to perfect the regeneration system of organogenesis means. The invention has the advantages that based on the selection of effective disinfection technology for soybean seeds, the improvement and the optimization of various medium compositions is outstanding; through the verification of the tissue culture of conventional soybean varieties, the induction, the elongation and the rooting of multiple shoot are effectively promoted; the quality of aseptic seedlings and the differentiation rate of the multiple shoot are improved; the organogenesis and plant regeneration process of the cotyledon node of majority varieties are perfectly realized; solid technical support is provided for the genetic transformation and the molecular improvement of soybeans; and the invention has important practical significance to the effective breeding and health production of the soybeans in China.

This invention relates to a restructured meat product, comprising; (A) a fibrous material containing soy protein and soy cotyledon fiber, wherein said soy cotyledon fiber is present in the fibrous material in an amount of from 1% to 8%, by weight on a moisture free basis; (B) a comminuted meat; and (C) water. In another embodiment, the invention discloses a process for preparing a restructured meat product, comprising the steps of; hydrating (A) a fibrous material containing soy protein and soy cotyledon fiber, wherein said soy cotyledon fiber is present in the fibrous material in an amount of from 1% to 8%, by weight on a moisture free basis in water until the water is absorbed and the fibers are separated; and adding (B) a comminuted meat, wherein the temperature of the comminuted meat is below 10° C.; and mixing the fibrous material and the comminuted meat to produce a homogeneous, fibrous and texturized meat product having a moisture content of at least 50%.

The invention provides a long grained seed steering precision seeding device comprising a plug conveyer belt, a directional seed delivering device, a directional seed dividing mechanism and an air-aspiration seeding mechanism, wherein the directional seed delivering device and the direction seed dividing mechanism are connected and are arranged at the same side of the plug conveyer belt; the air-aspiration seeding mechanism is arranged above the plug conveyer belt; a seed delivering slide way is arranged in the tangential direction of the edge of the directional seed delivering device; the seed delivering slide way and the directional seed dividing mechanism are connected; and the plug conveyer belt, the directional seed delivering device, the directional seed dividing mechanism and the air-aspiration seeding mechanism are fixed on a stand. The invention also provides a seeding method for realizing the seeding device; long grained seeds are delivered and absorbed into the hole of a plug respectively by the directional seed delivering device, the directional seed dividing mechanism and the air-aspiration seeding mechanism, thereby realizing precision seeding. The invention can provide seedlings with even germination and uniform cotyledon direction for melon crop full automatic engrafting devices and other equipment and has the advantages of high work productivity, stable work quality and the like.

The present invention provides methods for transforming monocot plants via a simple and rapid protocol, to obtain regenerated plants capable of being planted to soil in as little as 4-8 weeks. Associated cell culture media and growth conditions are also provided, as well as plants and plant parts obtained by the method. Further, a method for screening recalcitrant plant genotypes for transformability by the methods of the present invention is also provided. Further, a system for expanding priority development window for producing transgenic plants by the methods of the present invention is also provided.

The invention relates to a Folium Broussonetiae tea and the preparation method thereof. The Folium Broussonetiae tea is prepared from raw materials including 80-95 weight parts of Folium Broussonetiae and 5-20 weight parts of Flos Lonicerae (or Folium Nelumbinis or Folium Cassiae) by cleaning, sun-drying to semi-dryness, and deactivating enzyme in boiled water or in a tea green-removing machine for 30-120 seconds; rapidly cooling in cold water; drying for removing the water from the surface of the Folium Broussonetiae; kneading until the water content reaches 30-40%, and storing; treating the Flos Lonicerae by adopting the same method as the Folium Broussonetia; and mixing the kneaded Folium Broussonetia and the kneaded Folium Broussonetia at the above ratio, inoculating to Aspergillus niger liquid and yeast liquid, fermenting at 25-40 DEG C for 12-48 hours, and kneading and drying.

Methods and compositions to increase Agrobacterium transformation efficiency (frequencies) in both dicot and monocot host plants include adding histones to the host plant at most transiently, and using histones and L-cysteine at certain stages in monocot transformation.

Methods and compositions for increasing the starch content in green tissues of a plant are provided. The method comprises down-regulating me activity of starch degradation enzymes in a plant. The resulting transgenic plants of the invention have increased starch content in green tissues and exhibit a starch excess phenotype. In one embodiment the method involves manipulating a monocot plant to down-regulate the activity of a starch degradation enzyme. The plants are useful for improving the yield of free sugars from plant biomass and increase dried green tissue storage stability.

The invention relates to a method for high-frequency regenerating Chinese sapium bark through tissue culturing adventitious bud. The invention is characterized in that it comprises following steps: removing seed coat from Chinese sapium bark's seed, disinfecting surface with hydrogen dioxide solution and inoculating seed germinating culture medium, and getting intact Chinese sapium bark seed seedling; cutting the cotyledon of seed seedling, apical bud containing undeveloped true leaf and hypocotyl and root, and then inoculating them into adventitious bud inducing culture medium suitable for their growth, inducing adventitious bud directly or inducing with callus and getting light green adventitious bud cluster; replanting it into adventitious bud lengthening culture medium, getting lengthened adventitious bud seedling; replanting into seedling breeding culture medium for enlarged breeding, and replanting it into rooting culture medium, getting strong tube Chinese sapium bark with developed true leaf; finally replanting it out of room for natural growth and getting strong Chinese sapium bark seedling.

The present invention provides nucleotide sequences and corresponding amino acid sequences for plant cytokinin oxidase proteins. Also provided are vectors, host cells, and transgenic plants comprising such sequences as well as methods for stimulating root growth and / or enhancing the formation of lateral or adventitious roots and / or altering root geotropism using such sequences. Also provided by the present invention are methods for altering various plant phenotypes including increasing seed size and / or weight, embryo size and / or weight, and cotyledon size and / or weight using cytokinin oxidase proteins and / or nucleic acid molecules encoding cytokinin oxidase.

The present invention discloses the process of culturing adventitious root tissue of licorice, and belongs to the tissue culture of licorice as one kind of Chinese medicinal materials. The process of the present invention includes soaking licorice seed in solution with ethanol in 70-75 vol% and sodium hypochlorite in 2 vol% to sterilize and planting in MS culture medium to induce bacteria-free bud; inoculating the cut cotyledon and bud in MS culture medium containing different plant growth regulator to induce callus; transferring the callus growing for 20 days in MS culture medium with indolebutyric acid and kinin to induce adventitious root; and finally transferring the adventitious root to liquid culture medium containing the same plant growth regulator for fast growth. The present invention has simple process, high inducing rate, fast adventitious root proliferating speed and other features.

The invention discloses an easy and fast seedling method for hybrid pear seeds, which comprises the steps of fruit management, seed management and seedling management, wherein the fruit management is as follows: hybrid fruits can be collected in 5-10 days before morphological maturity and are aired and pre-arranged for 3-4h, and then are instantly arranged in a miniature mechanical refrigeratory for refrigeration under the temperature of 3-5 DEG C and a humidity of 85-95 percent; and the hybrid fruits can be stored until the first ten-day period of January in next year in the refrigeratory; the seed management is as follows: the hybrid fruits are delivered from the refrigeratory and are immediately cleaned and then are aired for 48h under a room temperature (0-10 DEG C); then the hybrid fruits are dipped for 0.5-1.0h by formaldehyde (formalin) 100 times liquid with a concentration of 40 percent; and then the hybrid fruits are directly sown without sand storage and lamination; and the seedling management is as follows: when the cotyledons of the seedlings are developed to two cotyledons and one core, the cotyledons are directly transplanted to a selective nursery in the current year with a permanent planting density of 2.0m*1.0m; and only the central trunks of the seedlings are maintained for extended growing so as to increase the height of crowns and try to enter the 'childhood length' of the adult stage as early as possible and shorten the childhood period.

The invention relates to a technology for grafting and transplanting of a high-yield and high-quality anti-seasonal eggplant, which comprises the following steps: (1) a common eggplant is taken as a graft and Solanum torvum is taken as a rootstock; (2) the seeds of the Solanum torvum are used for raising seedlings in July, and the seeds of the eggplant are used for raising seedlings in August; when the seedlings of the Solanum torvum grow 7-9 true leaves and the seedlings of the eggplant grow 4-5 true leaves, the seedlings are taken out; (3) a growing point which is 60-80mm from the root of Solanum torvum is cut off, and the end of the rootstock is cut into a wedge shape; the eggplant seedling is cut into a bevel opening at the eggplant seedling 60-80mm above a cotyledon from bottom to top, and the wedge-shaped end of the rootstock is inserted into the bevel opening of the graft with cortex aligned, and then the graft opening is bound up and fixed to form a two-root seedling; (4) the two-root seedling is transplanted into a field while grafting; after 10-15 days upon transplanting, when the wounds of the rootstock and the graft heal, the graft root is cut from the bottom of the graft opening with only the root of the rootstock left. The technology can cause the anti-seasonal eggplants planted in autumn and winter to have more strong vitality and the ability against diseases and insect pests; in addition, the eggplant planted by the technology has high yield and high quality, the seedling raising method is simple, the grafting seedling rate is high, thus the technology can realize transplanting seedlings into the field while grafting, which reduces the land, labor labor and production cost required by seed bed management.

The invention relates to a convenient and rapid DNA extracting method of peanut health tissues and diseased tissues. The method comprises two steps of: sample materials preparation: testing the leaf and stem tissues, shell tissues or peanut cotyledons, the weight of which is 3-10 mg, adding the sample materials into a 1.5ml centrifugal tube preset with an alkaline lysate or enzymolysis liquid, and grinding by a grinding rod till the visible particles do not exist; DNA extracting liquid preparation: treating the ground sample materials by an alkaline decoction method or a enzymolysis method to obtain the DNA extracting liquid. The method is also suitable for peanut health tissues and diseased tissues. Compared with the DNA extracting method, the invention has short required time, the required time of single sample by the alkaline decoction method does not exceed 20min, and the required time of single sample by the enzymolysis method does not exceed 0.5h; the dosage of the sample is little, and each sample only needs 3-10mg; the method has no need of liquid nitrogen to grind, therefore the cost is low, and the requirements of peanut molecule marker auxiliary breeding and transgene breeding are satisfied. The invention has no influence on the plant growth or seed germination, and has significant meaning on the practicality of peanut molecule breeding technology.

A novelty item and method of making same, include a non-germinated dicot seed with a word, symbol, or the like, etched through the seed coating to the cotyledon which remains visible from the time the seedling sprouts through the surface of the soil throughout the germination period.

The invention discloses a micropropagation method of fraxinus rhynchophylla and relates to the micropropagation method. The problems of long reproductive cycle, low reproduction efficiency and poor offspring genetic stability in nursery stock propagation of fraxinus rhynchophylla are solved. The method comprises the following steps of: 1, sterilizing fraxinus rhynchophylla seeds, and culturing single cotyledons of zygotic embryos to obtain cotyledonal cell embryos; 2, performing maturation cultivation on the cotyledonal cell embryos; 3, performing sprouting cultivation on the cotyledonal cellembryos subjected to maturation cultivation to obtain regenerated plants; and 4, transplanting the regenerated plants into a culture medium to culture until new leaves are completely unfolded, and removing a covered plastic thin film. The micropropagation method has the advantages of short nursery stock reproductive cycle, high reproductive rate (culturing for 60 to 70 days) and high reproductionefficiency (each explant can generate 15 to 20 somatic embryos); the germination rate of the somatic embryos is up to 87 to 89.55 percent; the transplanting survival rate of the regenerated plants isup to 75 to 80 percent; and the regeneration plants with the same good characteristics as female parents can be generated in mass.

The invention discloses an end executor of a potted vegetable seedling supply mechanical hand. The end executor comprises a step motor, a shaft coupling, a supporting plate, supporting rods, a lead screw, a spiral slide sleeve, a lifting block, connecting rods, guide rods, a guide plate, a seedling withdrawal plate and seedling shovels, wherein two ends of each of the four supporting rods are connected with the supporting plate and the guide plate respectively, and two ends of the lead screw are mounted in the center of each of the supporting plate and the guide plate through bearings and connected with an output shaft of the step motor through the shaft coupling; the seedling shovels are fixed on the guide rods, the four guide rods penetrate through the guide plate and form a moving pair together with the guide plate, one end of each of the four connecting rods is connected with the lifting block through a hinge, and the other end of each of the four connecting rods is connected with each of the four guide rods through a hinge; the seedling withdrawal plate is fixed below the guide plate. The four seedling shovels extend into a hole to basically form a wrapped-type structure, so that a loose seedling substrate or a cotyledon-stage potted seedling in the hole can be completely taken out; same-age cotyledon-stage potted seedlings are moved into holes through cooperation of the seedling shovels and the seedling withdrawal plate, so that the seedling supply is realized, the seedling-raising efficiency is improved, and a technical support is provided for automated transplantation of potted vegetable seedlings.

The invention discloses a herbal composite and a preparation thereof for treating gynecological inflammation. The herbal composite is prepared of cotyledon, selinum, sophora, phellodendron, radix Stemonae, agrimonia, sanqi, borax, borneol; the weight ratios are that 3-15 portions of cotyledon, 3-15 portions of selinum, 3-15 portions of sophora, 2-10 portions of phellodendron, 2-10 portions of radix Stemonae, 2-10 portions of agrimonia, 0.2-2 portions of sanqi, 3.03-0.3 portion of borax, and 0.06-0.6 portion of borneol. The preparation can be suppository, gel, cream, solution agent, film, spray, effervescent tablets agent, and other externally used preparations. The invention adopts common herbal medicines as materials, the usage cost of a sufferer is lower, the content of the effective components are high, the stability is high, and the curative effect is notable. The herbal composite and the preparation of the invention can reduce fever, destroy the toxic properties, dry dampness, kill insect, remove rot, and produce fresh, can inhibit and kill pathogenic micro-organisms and infusorium, can improve the immunity of organism, can cure tickle and relieve pain, can accelerate metabolism, can promote the tissue to rebirth. The prescription is applicable for the sufferers with leukorrhea with reddish discharge, pruritus vulvae, cervical erosion, infusorium vaginitis, mucedine vaginitis, nonspecific vaginitis, etc.

The invention relates a broad bean paste, comprising bean cotyledon mother paste and burden. The bean cotyledon mother paste is made by solarizing the leavening which is made by fermenting the bean cotyledon with flavomycoin, and the burden comprises saccharose, peppertree, fresh chilli, white spirit, and soya bean flour, glutinous rice wine, cooking vegetable oil, aromatic oil and natural plant perfume. The broad bean paste contains the local flavor burden. The method comprising the following step: rinding the broad bean and soaking it with cool water, adding flavomycoin and mixing, at 28-45Deg.C and 50-90% relative humidity, carrying out aerobic fermentation for 15-25 days, adding common salt and solarizing, getting the bean cotyledon mother paste; adding the burden into the bean cotyledon mother paste and mixing them. The invention also contains the steps of adding local flavor burden.

The present invention relates to herbicide composition of monosulfuron ester compound as well as its application, which is compounded with two-element or three-element from herbicide monosulfuron ester or its natrium salt (0.1 to 90 percent) as main active component, and other herbicide (0.1 to 85 percent) of ethyl carboxylic fluorine grass ether, Starane, thifensulfuron-methyl, tribenuron-methyl, 2,4D as well as its ramification, Prometrex prometryn, acetochlor, atrazine, triallate, metolachlor, bensulfuron-methyl or 2 armour 4 chlorin natrium etc., and the herbicide composition can be made into breast oil, wettable powder, soluble powder, water emulsion, microemulsion agent, aqua, suspending agent, microcapsule agent or water disperse particle agent by adding proper amount auxiliary agent and carrier accepted in agriculture. The composition can be used to prevent single and double cotyledon weed in wheat, corn and millet field.

The invention discloses lithocarpus polystachyus Rehd health-care tea and a preparation method thereof. The tea is prepared from, by weight, 60-80 parts of lithocarpus polystachyus Rehd, 10-15 parts of broadleaf holly leaves 8-15 parts of panax notoginseng leaves, 5-10 parts of semen cassiae leaves and 6-10 parts of lotus leaves. The preparation method of the health-care tea includes the steps of raw material preprocessing, weighing and mixing, spreading and airing, fixation, rolling, fermentation, secondary fixation, drying, sterilization and packaging. The prepared health-care tea is good in flavor, good in taste, moderate in sweetness and low in calorie, and no additives are added. Meanwhile, the tea has a good tea medicine function, has the functions of reducing blood sugar and blood lipid, making people lose weight, reducing blood pressure, clearing away heat and toxin, protecting liver and kidney, resisting oxidization, delaying ageing, inhibiting and preventing cancer, improving organism immunity and the like, can be drunk for a long time, and is highly popular with mass consumers.

The invention provides a preparation method of soybean milk. The preparation method includes 1) adopting soybean cotyledon particles subjected to breaking, embryo removal, fine powder removal and soybean skin removal to serve as a raw material; 2) soaking the soybean cotyledon particles in water for 30-60 minutes, milling the soybean cotyledon particles to obtain raw soybean milk after removing bean dregs; 3) boiling the raw soybean milk, and maintaining temperature at over 85 DEG C for 30-45 minutes to obtain the soybean milk. Hardness of the water for soaking the cotyledon particles is 2-4.5 mmol / l, and water temperature is at room temperature. The pH value of the raw soybean milk is adjusted to 6.6-7.2 prior to boiling or in the boiling process; and preferably, the pH value of the raw soybean milk is adjusted to 6.8-7.0, and the pH value is adjusted through NaOH or KOH. The preparation method of soybean milk shortens soaking time of soybean, and obtains the soybean milk which is white in color and good in creaming state while saving investment and reducing energy consumption. When the soybean milk produced through the preparation method is used for production of bean curd, the yield rate, water-retaining property and hardness of the bean curd are greatly improved.

The invention discloses a method for grafting and raising a seedling of a melon, and relates to the technical field of melon cultivation. The method comprises the steps of (1) selecting a suitable stock variety and a suitable scion melon variety; (2) grafting by adopting a double-root-cut-grafting method when the first main leaf of a stock seedling appears till the first main leaf just spreads, and the cotyledon of the scion seedling begins to turn green till the cotyledon spreads, then carrying out cuttage in a float breeding disc filled with a matrix; (3) floating the grafted seedlings in a seedbed with clear water, carrying out management of healing and root growing; and (4) injecting a nutrient solution into the seedbed after the grafted seedling survives, and floating the grafted seedling in the nutrient solution for survival post-treatment. The grafted seedling cultivated by adopting the method is strong and regular, has an extensive root system and the strong capacity in absorbing water and fertilizers, is fast in rejuvenation after being planted, grows strongly and is difficult to wane early.

The invention discloses a method for separating and converting rape protoplast. The method comprises the following steps: 1, selecting rape cotyledons or true leaves, and removing lower epidermis; 2, putting into enzymolysis liquid for carrying out enzymolysis, wherein the enzymolysis liquid is prepared from the following formula components: 1wt% of cellulase, 0.3wt% of macerozyme, 0.5M of mannitol, 20mM of KCl, 20nM of MES with a pH value of 5.7, 10mM of CaCl2 and 0.1wt% of BSA; 3, adding an isovolumetric W5 solution into a mixed solution obtained by means of enzymolysis, carrying out low speed centrifugation for removing supernatant, adding W5 for washing and resuspending, adding resuspended liquid into 20wt% of a sucrose solution for purifying, horizontally centrifuging, and taking supernatant green protoplast liquid; 4, centrifuging for removing the W5 solution, and resuspending protoplast precipitate by using an MMG solution; 5, converting the protoplast. The protoplast separating and converting method is established in rape for the first time; the rape protoplast is used as a receptor for converting, so that yellow fluorescent protein (YFP), luciferase (Luc) and renilla reniformis luciferase (Ren) are successfully expressed.

The present invention relates to improved methods for producing transgenic sunflower plants. Specifically, methods are disclosed for transforming sunflower tissues and regenerating fertile transgenic plants therefrom. The methods are particularly amenable to sunflower tissues, such as cotyledons, which have proven refractory to previous methods of transformation and regeneration.

The invention relates to a method for inducing a high-frequently generating of cross-breeding liriodendron liquid suspended somatic embryo by solid-liquid alternate culture and cross-breeding liriodendron regeneration. After an immature zygotic embryo from ball-shaped stage to cotyledon stage is obtained from separating, and goes through embryonic callus induction stage, embryonic callus propagation stage, the stage of the embryonic callus tissue suspension cell system establishing and pre-multiplication, embryonic suspension cell multiplication stage, the stage of stopping embryonic suspension cell multiplication and forming of embryo, somatic embryo development stage and somatic embryo plant regenerating stage, the regenerated plant of cross-breeding liriodendron can be obtained.

The invention discloses an agrobacterium-mediated jatropha curcas gene transformation method. A material after 1 / 3 to 1 / 4 of base is cut from the cotyledon of a mature seed of jatropha curcas is used as an agrobacterium infection receptor. The method comprises the following steps of: after co-culturing, inoculating the transformed receptor and the agrobacterium to a culture medium without a screening agent to perform callus induced culture for 4 weeks; and succeeding the receptor and the agrobacterium to a selective culture medium and screening resistant buds by using kanamycin sulfate as a screening agent, sub-culturing the resistant buds obtained by screening to obtain resistant plants, and obtaining transgenic jatropha curcas plants by molecular detection. Compared with the conventional jatropha curcas transformation method, the method can obtain high regenerative bud induction rate, simplifies the transformation program, shortens the transformation period, greatly improves the transformation efficiency and has good application prospect.