The invention relates to a new method for avoiding microalgae photoinhibition and improving astaxanthin yield in the culture process of microalgae. In general, a two-stage method is adopted to cultivate the microalgae to produce astaxanthin, wherein on the first stage, massive reproduction of cells and accumulation of biomass are achieved in a heterotrophic, autotrophic or mixotrophic culture mode, and on the second stage, accumulation of the astaxanthin in the cells are achieved in methods, such as light stress assisted with nutrition stress. However, when the microalgae is transformed from the firsts stage to the second stage, phenomenons, such as light stress usually occur, and accordingly the astaxanthin yield is seriously influenced. Therefore, multiple methods are adopted to control culture conditions in a transformation process, such as medium components controlling, sunshading, weak light transition, initial inoculated density improving, gradual dilution and outdoor inoculated time adjusting so that cell conditions can be adjusted and the cells can improve the ability to resist a strong light; therefore, thelight stress problem of the microalgae under the strong light is solved completely, the efficiency of the microalgae for producing the astaxanthin is improved greatly, and low cost, high efficiency and large-scale cultivation of the microalgae for producing the astaxanthin are achieved.