Bean metalloprotease pvftsh2 gene and its encoded protein and application
A metalloprotease and gene technology, applied in the field of genetic engineering, can solve problems such as the decline of photosynthetic function, and achieve the effect of increasing the yield of kidney beans and improving the utilization rate of light energy
Active Publication Date: 2021-12-31
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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Problems solved by technology
[0003] Light is a necessary factor for chloroplast development and photosynthesis, but under high light intensity conditions, light energy exceeds the amount that the photosynthetic system can utilize, a by-product of plant photosynthesis 1 o 2 Sustained damage to the D1 protein in the photosystem Ⅱ reaction center, resulting in a decline in photosynthetic function
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specific Embodiment approach 1
[0036] Embodiment 1: The nucleotide sequence of the phase bean metalloprotease PvFtsH2 gene in this embodiment is shown in SEQ ID NO: 1 in the sequence listing.
specific Embodiment approach 2
[0037] Specific embodiment 2: The amino acid sequence of the protein encoded by the bean metalloprotease PvFtsH2 gene in this embodiment is shown in SEQ ID NO:2.
specific Embodiment approach 3
[0038] Specific embodiment three: the application of the bean metalloprotease PvFtsH2 gene in regulating the photoinhibition response process of leguminous plants in this embodiment.
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Abstract
The bean metalloprotease PvFtsH2 gene and its encoded protein and application relate to the field of genetic engineering, in particular to a plant metalloprotease PvFtsH2 gene and its encoded protein and application. The invention provides a bean FtsH metalloprotease PvFtsH2 gene, its encoded protein and application. The nucleotide sequence of the PvFtsH2 gene is shown in SEQ ID NO: 1 in the sequence listing. The amino acid sequence of the encoded protein is shown in SEQ ID NO:2. The present invention confirms that the PvFtsH2 gene plays a role in the light-inhibition response process through plant physiology and molecular biology methods, and the mutation of the gene leads to F under light-inhibition conditions. V / F M The value decreased, the chlorophyll content decreased, and the D1 protein accumulated. The invention has important application value in increasing the yield of kidney beans by improving the utilization rate of light energy.
Description
technical field [0001] The invention relates to the field of genetic engineering, in particular to a plant metalloprotease PvFtsH2 gene and its encoded protein and application. Background technique [0002] Bean production is group production, and bean production also refers to group production. There are two main ways to increase the yield of kidney beans: one is to increase the absorption of the root system. A strong root system and a large absorption surface area are fundamental to maintaining the water and nutrients required by the stems, leaves, flowers, fruits and other organs on the ground; the other is to increase photosynthesis. 90% of the output of leguminous crops comes from photosynthesis, and the rate and efficiency of photosynthesis directly determine the yield of crops. Breeding with high light efficiency has long been a hot spot in the field of crop photosynthesis research and breeding. Improving the efficiency of light energy absorption, transmission, and c...
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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/57C12N9/50C12N15/82A01H5/00A01H6/54
CPCC12N9/63C12N15/8261C12N15/8251
Inventor 徐坤夏正俊朱金龙吴红艳翟红
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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