32 results about "Plant physiology" patented technology

The invention relates to the crossing field of agricultural intelligent algorithms and intelligent equipment technologies, in particular to an environment regulation and control model algorithm and algorithm model platform fusing crop physiology. According to the model algorithm, through four-step calculation of spatio-temporal heterogeneity adaptation, multi-source data dynamic coupling, stress self-adaptive adjustment and self-learning iterative optimization, a partition precise regulation and control decision value is generated; the algorithm model platform comprises a data acquisition layer (a partition sensor and a multi-source interface), a feature processing layer (a three-dimensional feature library and data preprocessing), a model algorithm layer (deploying the model algorithm) and a platform application layer (partition regulation and control and closed loop feedback), and full-link cooperation of algorithm calculation, platform execution, effect feedback and algorithm iteration is achieved. The crop yield is increased by 15-20%, water resources are saved by 25-30%, and the method is suitable for multi-crop facility agriculture scenes.

The invention relates to the crossing field of agricultural information technology and urban sustainable development, and discloses an urban micro-agriculture three-dimensional edible landscape intelligent low-carbon benefit evaluation and adjustment and optimization method and an urban micro-agriculture three-dimensional edible landscape intelligent low-carbon benefit evaluation and adjustment and optimization system. According to the method, data are collected through a multi-modal behavior sensing device and a plant physiology monitoring array, a behavior-ecology synchronous time sequence set is constructed, a plant growth dynamic simulation model fusing behavior disturbance factors is established, carbon sink, energy consumption and food output efficiency are quantified, and a plant growth dynamic simulation model is established. And based on a multi-target particle swarm optimization algorithm, generating a spatial layout and interaction strategy combined adjustment and optimization scheme. The system comprises a behavior perception module, an ecological monitoring module, a space-time alignment module, a model simulation module, a benefit calculation module and a visual pushing module. According to the method, the low-carbon benefit evaluation precision and the community participation sustainability are remarkably improved, the actual measurement prediction error is reduced by 42%, and the comprehensive benefit is improved by 28%.

The present application relates to the field of plant physiology and ecology and plant chemistry, and particularly relates to a method for improving the content of gypenoside R2 in the root system of Suaeda by salt stress and application. The Suaeda plants are stressed by 100-300 mmol / L NaCl solution for 28 days, and the root system is collected to obtain the raw material rich in target saponins. The method can significantly improve the relative content of target saponins gypenoside R2 in the root system of Suaeda. Compared with the control group, the low-salt treatment can increase by 65.34%, the medium-salt treatment can increase by 161.83%, and the high-salt treatment can increase by 240.84%.

The invention relates to a salt and alkali tolerant plant growth promoting enterobacter and application thereof, and belongs to the technical field of microorganism application and agricultural biotechnology, the salt and alkali tolerant plant growth promoting enterobacter is Enterobacter cancerogenic enterobacter PGPR AT1, and the bacterial agent contains the Enterobacter cancerogenic enterobacter PGPR AT1. The growth-promoting enterobacter is applied to plant growth promotion under severe saline-alkali stress conditions or normal growth conditions. The salt-alkali tolerant plant growth promoting enterobacter can directly or indirectly synergistically promote microorganisms for crop growth, and can be prepared into an efficient and stable microbial agent. The microbial agent can achieve a synergistic effect from three aspects of soil, root systems and plant physiology, the physiological stress of crops in the saline-alkali soil is remarkably relieved, the yield, biomass and stress resistance of the crops are fundamentally improved, and long-acting and sustainable improvement on the saline-alkali soil is achieved.

The application discloses a plant intelligent factory multidimensional data monitoring and collaborative control method and system, and belongs to the technical field of collaborative control. The method specifically comprises the following steps: collecting multidimensional data of a plant intelligent factory in real time, wherein the multidimensional data comprises plant physiological parameters, environmental factor parameters, nutrient solution parameters, and device and operation state parameters; based on the multidimensional data, a dynamic multi-level causal correlation model between devices, plant physiology, environment and operation is constructed; potential collaborative influence paths in an abnormal state are identified; when an abnormality is detected or a growth process deviates from a trend is predicted, cross-level control is automatically performed; cross-level control instructions are distributed to the device level, the cultivation unit level and the workshop level according to the levels; and the plant intelligent factory is subjected to multi-link joint regulation and control. Through the establishment of the collaborative mechanism of the causal correlation and the path, the regulation and control are more accurate, flexible and adaptive, and the stability, resource utilization rate and intelligent level of plant production are effectively improved.

The invention belongs to the technical field of plant physiology monitoring, and provides a kale seedling stage drought stress identification modeling method, which comprises the following steps: selecting seedlings with uniform growth states, averagely dividing the seedlings into a control group and a light / medium / heavy drought group, and collecting images marked with nine fluorescence parameters of each group of seedling leaves. Based on the parameters, five kinds of relations and constraints of PSII energy distribution conservation, quenching complementation and the like are established, the first two kinds of constraints are converted into constantly established hard constraints, the last two kinds of constraints are converted into differentiable soft constraints, and photosynthetic physiological constraint loss is jointly formed; and constructing related prototype alignment and directional constraints in combination with a collaborative response mode of parameters under normal and drought stress to form collaborative constraint loss. And on the basis of task loss, fusing two types of constraint loss and giving a weight to obtain a total loss function, thereby constructing a drought stress identification model. The technical problems that a pure data driving model is lack of interpretability, weak in generalization ability and poor in stability are solved.

The utility model discloses a water level regulation and control experimental facility relates to water level regulation and control technical field, including planting box, water storage tank, liquid level sensor and control unit, plant is planted in the bottom of planting box, the planting box is equipped with drainage pump and liquid level sensor, drainage pump is communicated with water storage tank through drainage pipe, water storage tank is equipped with water inlet pump, water level sensor is equipped with water inlet pump. The water storage tank is used for containing a water source, the water inlet pump communicates with the planting box through a water inlet pipe, and the liquid level sensor, the drainage pump and the water inlet pump are in communication connection with the control unit. The liquid level sensor in the level regulation and control experimental device can display the water level in the planting box in real time, the planting box is communicated with the water storage box through the water pump, the control unit can regulate and control the water level in the planting box in real time according to the reading of the liquid level sensor, quantification and automatic control of water flooding conditions are ensured, manual monitoring is not needed, and the labor intensity of workers is reduced. A reliable tool is provided for plant physiology and ecology research.

The invention relates to the field of plant physiological ecology and phytochemistry research, in particular to a method for increasing flavonoid substances in suaeda salsa root exudates under saline-alkali stress and application. And carrying out stress treatment on the suaeda salsa plant for 6-8 weeks by using a NaCl solution with the concentration of 50-300 mmol / L or an alkali solution with the pH value of 8.0-9.0, and collecting a culture solution to obtain the root exudates rich in target flavonoid substances. According to the method, the relative content of the target flavonoid substance 3, 5-Di-O-methyl-8-prenylafelectin-4beta-ol in the suaeda salsa root exudates can be remarkably increased, and compared with a control group, the relative content of the target flavonoid substance 3, 5-Di-O-methyl-8-prenylafelectin-4beta-ol in the suaeda salsa root exudates can be increased by 44.64% through low-salt (100 mmol / L NaCl) treatment and can be increased by 28.9% through high-alkali (pH 9.0) treatment.

The invention relates to the technical field of agricultural breeding, in particular to green agricultural ecological breeding equipment and a breeding method.The green agricultural ecological breeding equipment comprises a breeding shell, an operation opening is formed in one side of the breeding shell, an opening and closing door is slidably arranged on the operation opening, and a rainproof ceiling is arranged at the top of the breeding shell through a plurality of supporting columns; a mounting cover is arranged in the center of the bottom of the breeding shell; the rotating shaft is controlled to rotate through the driving motor, then the illuminating lamp ring is controlled to move up and down, the track of the sun is accurately simulated, the natural-imitated illumination rhythm better conforms to plant physiology, green seedlings which are higher in stress resistance and more robust can be cultivated, meanwhile, due to the arrangement of the cultivation grooves, breeding matrixes and seeds can be conveniently borne, and the breeding efficiency is improved. The seed seedlings can uniformly receive light.

The invention discloses a plant physiology monitoring and irrigation control system and method and a readable storage medium, and the system employs an ultrasonic sensor to obtain an acoustic emission signal generated by a plant, and carries out the processing of the acoustic emission signal, so as to obtain acoustic characteristics representing the acoustic characteristics of the plant. A multi-modal physiological sensor is adopted to obtain a plant physiological signal and process the plant physiological signal to obtain physiological characteristics representing plant physiological characteristics; and then fusing the acoustic features and the physiological features by using a multi-modal fusion model to obtain a plant feedback index (PFItotal) representing the internal stress state of the plant, predicting a future plant stress trend by using a prediction model, and finally feeding back a stress trend prediction result for controlling an irrigation decision to realize adaptive irrigation adjustment. According to the system, irrigation control can be directly, autonomously and accurately carried out according to physiological needs of plants, early-stage stress detection and control can be achieved several hours before visible symptoms appear, and water consumption can be reduced according to plant types and climate conditions.

The present application relates to the technical field of agricultural sensor, and particularly relates to a PVA and HAMA composite hydrogel microneedle for crop monitoring and a preparation method thereof. The microneedle is composed of a microneedle layer formed by photo-crosslinking and a back layer formed by physical crosslinking. The preparation method comprises the following steps: preparing a photoinitiator solution; preparing and defoaming a HAMA solution; adding the HAMA solution into a PDMS microneedle mold, centrifugal filling, temperature control concentration and violet light curing to form the microneedle layer; then adding a PVA solution to the back of the microneedle and drying to obtain a microneedle array. The obtained microneedle has good mechanical strength, water absorption and biocompatibility, can penetrate the cuticle layer of plant leaves or stems under low damage conditions, and realizes the collection of trace liquid in the plant body. The microneedle can be used as a sensing substrate for monitoring pH, conductivity, moisture and ions, and can realize real-time non-invasive monitoring of the physiological state of crops. The present application has simple process, low cost and can be prepared on a large scale, and is suitable for the fields of smart agriculture, precision management and plant physiology research.

The invention discloses a blueberry seedling growth simulation model construction and decision optimization method, and relates to the technical field of blueberry seedling, a blueberry seedling region is gridded, and each grid is provided with a blueberry growth monitoring module and an environmental water and fertilizer response device. The blueberry growth monitoring module comprises an environmental parameter monitoring unit, a plant physiology monitoring unit, a root system monitoring unit and an edge data acquisition and analysis unit; the dynamic blueberry seedling raising model comprises a fusion mechanism model photosynthesis sub-model, a respiration sub-model and a root growth sub-model. According to the invention, the multi-dimensional monitoring module is deployed in a gridding manner, cooperative sensing of the seedling raising environment, the plant physiology and the root system state is realized, the problem of fragmentation of traditional monitoring is solved, and the monitoring precision is greatly improved. And the multi-mechanism sub-models are coordinated and linked, so that the growth state prediction deviation is greatly reduced, and dynamic quantitative pre-judgment of indexes such as biomass and root development is realized.

The invention discloses a leaf sensor patch for monitoring plant physiology as well as a preparation method and an application method thereof, and belongs to the technical field of plant sensing. The blade sensor patch comprises a flexible substrate, a silver nanowire cross electrode and a sensor group; the sensor group comprises at least one resistance-type volatile organic compound sensor, at least one resistance-type blade temperature sensor, at least one capacitance-type blade surface humidity sensor and at least one capacitance-type environment humidity sensor; the silver nanowire cross electrode is embedded into the flexible PDMS substrate, the sensor group is integrated on the surface of the flexible PDMS substrate, and all sensors in the sensor group are connected with the silver nanowire cross electrode. Synchronous monitoring of volatile organic compounds of leaves, surface temperature / humidity and environment humidity is achieved, high sensitivity and selectivity are achieved, and the problem that an existing plant physiology monitoring sensor cannot synchronously monitor plant biochemical signals and biophysical signals can be effectively solved.

The invention relates to the technical field of environmental monitoring and plant physiology ecology, in particular to an assimilation box device for single plant carbon sink metering, which comprises a base sleeved on a single plant, the top surface of the base is fixedly connected with a support frame, and one end of the support frame away from the base is detachably connected with a box body. An air inlet assembly and an air outlet assembly are arranged on the upper portions of the opposite side walls in the box body respectively, trays are arranged on the lower portions, extending out of the box body, of the air inlet assembly and the air outlet assembly respectively, wiring holes communicated with an inner cavity of the box body are formed in the trays, and circulating fans used for enabling air in the box body to flow circularly are arranged at the opposite corners of the box body respectively. The box body is detachably connected with the supporting frame, so that the portability and the rapid deployment capability of field operation can be effectively considered, and a target single plant can be accurately isolated and measured; and non-uniform gas distribution in the box body can be avoided through the gas inlet assembly, the gas exhaust assembly and the circulating fan, so that the measurement precision is effectively improved.

The invention provides a plant disease and insect pest detection-diagnosis-regulation method and system, and relates to the technical field of intelligent agriculture and forestry management. According to the method, multi-source monitoring data such as plant images, soil physicochemistry, plant physiology and environmental meteorology are integrated, comprehensive diagnosis and prediction are carried out based on the AI analysis model, the limitation of single data source analysis is overcome, and the early recognition accuracy and early warning timeliness of plant health conditions and plant disease and insect pest risks in a complex environment are remarkably improved. Through cross validation and complementation of multi-dimensional data, misjudgment and missed judgment are reduced, the diagnosis result is more comprehensive and reliable, and a solid data foundation is laid for precise regulation and control.

The utility model relates to the technical field of plant physiology and ecology data collection, in particular to a robot capable of automatically placing and recycling a gas collection box. The utility model discloses a robot for automatically placing and recycling a gas collection box. One end of a trolley base is fixedly connected with a radar control tower, and the radar control tower is used for fixing a laser radar; an electric suction cup and a depth camera are mounted at one end of the six-axis mechanical arm, and the other end of the six-axis mechanical arm is fixedly connected with the other end of the trolley base through a metal connecting piece and used for placing and / or carrying the gas collection box in a non-coaxial mode; the gas collection box is arranged at a reserved position of the trolley base and is used for collecting a gas sample; the depth camera is used for collecting image information; the electric suction cup is used for adsorbing the gas collection box to realize placement and recovery of the gas collection box, and the laser radar is used for scanning an actual scene to generate a 2D map. According to the utility model, the six-axis mechanical arm and the depth camera are adopted, so that the accurate placement and recovery of the gas collection box are realized, the labor cost is reduced, and the working efficiency is improved.

The invention discloses a Clematis florida ClLEA coding sequence and application thereof, and belongs to the fields of molecular biology, plant physiology, gene engineering and the like. According to the invention, a ClLEA coding sequence is screened and expressed in clematis, so that the water logging tolerance of arabidopsis thaliana is remarkably improved. The gene and the protein thereof provided by the invention can be applied to genetic improvement of plants, and an effective technical means is provided for cultivating new varieties of crops, horticultural crops and the like with enhanced waterlogging tolerance.

The invention discloses a method for selectively extracting plant xylem embolism conduit juice, and belongs to the technical field of plant physiology research. In order to solve the problem that pure embolism catheter juice cannot be distinguished and obtained in the prior art, a plant stem section in a specific water stress state is obtained, the morphological lower end of the plant stem section is connected with a hydrophobic extracting solution, the upper end of the plant stem section is connected with a collecting system applying 0.01-0.1 MPa negative pressure, the extracting solution is driven by the negative pressure to selectively enter and flow through an embolism catheter, and the juice in the embolism catheter can be obtained. Meanwhile, due to the fact that the applied pressure is far smaller than the pinhole membrane fixing force borne by a water column in the functional catheter, the juice in the functional catheter can be reserved. Specific extraction of embolism catheter juice is realized for the first time, and a reliable method is provided for directly analyzing physicochemical properties of the juice and researching an osmotic regulation mechanism of xylem embolism repair.

The invention discloses a mangrove forest big tree transplanting method for a dredging disturbance area, which comprises the following steps: trimming a selected big tree crown, smearing a wound healing agent, digging a soil ball by taking a trunk base as a center, placing the soil ball of the big tree into a root system container, filling and compacting with a nutrient medium, implanting microcapsules into the trunk, and transplanting the big tree into the root system container. Carrying out tolerance culture on the big tree in two stages, putting a root system container in which the big tree is planted into a planting pit of the dredging area, additionally arranging a temporary hollow triangular pyramid or net cage at the periphery of the big tree, and regularly checking the integrity of the root system container, the growth condition of the root system and the physiological state of the big tree. According to the transplanting method, a revolutionary forest nutrition conveying mode is created through deep integration of material science and plant physiology, the transplanting method is particularly suitable for dredging areas and other scenes where the root system environment is severe and traditional maintenance is difficult, the survival rate and the recovery speed of mangrove forest big tree transplanting can be remarkably increased, and the survival rate of mangrove forest big tree transplanting is increased. The device is an edge tool for precise forestry and difficult site ecological restoration in the future.

The present application relates to the field of plant physiology and biomarker, and particularly relates to a cell membrane lipid marker combination for characterizing cold tolerance of citrus fruits and application in detection. The present application provides a set of cell membrane lipid marker combination for characterizing cold tolerance of citrus fruits, which comprises 39 kinds of membrane lipid components such as PC(O-38:5), ShexCer(35:0;20), PE-Cer(41:6;20), LPE(36:3), PC(32:0), TG(16:0_18:0_18:1), LPC(20:3) and the like. The process for extracting, purifying and detecting the membrane lipid components of citrus fruits is established, and a cell membrane extraction and detection kit convenient for small sample operation is developed, so as to provide a rapid and simple evaluation method for breeding of cold-tolerant varieties of citrus fruits and selection of reasonable storage temperature after harvesting.

The invention provides an arabidopsis thaliana root system alternating current impedance detection system and method based on a micro-fluidic chip, and relates to the technical field of alternating current impedance measurement. The system comprises a micro-fluidic chip, an AD5933 detection module, a single chip microcomputer and an upper computer. The micro-fluidic chip is formed by bonding a PDMS layer and a glass substrate, a channel for growth of an arabidopsis thaliana root system is arranged in the micro-fluidic chip, and a detection electrode is integrated on the substrate. Arabidopsis thaliana seeds are cultured in a chip channel, after root systems of the seeds grow to cover electrodes, an AD5933 module is used for applying small-amplitude alternating current signals and measuring root system impedance, and data are uploaded to an upper computer through a single-chip microcomputer to be processed. The method realizes in-situ, non-destructive and low-cost electrical property detection of the living arabidopsis thaliana root system, overcomes the limitation of traditional destructive sampling, can be combined with an optical microscope for synchronous morphological observation, and provides an efficient technical means for plant physiology research.

This invention relates to the field of soil fertility technology, specifically to a method and system for comprehensive detection of the fertilizer efficacy of multi-element compound fertilizers in southern double-cropping rice areas. The method includes the following steps: obtaining the redox potential level to determine the reduction state classification; combining the diffusion constant to determine the nutrient release boundary; analyzing the ion antagonism strength to obtain the effective chelation potential energy; using the xylem pressure difference to determine the vascular bundle node transport conductance parameters; analyzing the release rate and chelation potential energy to determine the theoretical nutrient flux; determining the physiological intensity through calcium ion pulses; comparing deviations; and calculating a score. In this invention, by coupling soil reduction classification, fertilizer release boundary, rhizosphere chelation efficiency, and plant transport conductance multi-dimensional parameters, a correlation model from soil environment to plant physiology is established. Real-time physiological feedback signals are used to perform dynamic weight allocation and normalization processing, improving the matching degree between evaluation results and actual growth response, and achieving high-frequency and accurate quantification of the efficacy of multi-element compound fertilizers.

The utility model discloses a panoramic phenology monitoring system, which comprises an image module, a sensor module, a master control module and a power supply module, the image module comprises a base and three camera shooting units which are arranged on the base and have the same model, the three camera shooting units are distributed at equal angles relative to the center, and the camera shooting ends of the camera shooting units all face the outside of the center; the sensor module comprises a plurality of plant physiological sensors and environment sensors; the main control module comprises a PLC (Programmable Logic Controller) and an upper computer communicated with the PLC; the camera unit and the sensor module are communicated with the upper computer through cables; the power supply module supplies power to the main control module. By adopting the above arrangement, the sensor can be linked to read environment and botany related data when a 360-degree panoramic image is shot, meanwhile, the continuity of the data can be ensured after the system needs to be maintained and the camera body needs to be replaced, and the visual angle difference does not occur.

The application provides a multispectral plant physiology-environment coupling monitoring wearable sensor and method, and belongs to the technical field of agricultural information sensing and plant phenotype monitoring. The multispectral plant physiology-environment coupling monitoring wearable sensor comprises an upper clamping arm and a lower clamping arm. The upper clamping arm comprises an upper base, an excitation light source arranged on the upper base and facing the upper epidermis of a leaf, two first multispectral detectors symmetrically arranged on both sides of the excitation light source, and an upper light guide layer covering the upper epidermis of the leaf. The excitation light source is located at the geometric center of the upper base. The lower clamping arm comprises a lower base, a second multispectral detector arranged on the lower base, and a lower light guide layer covering the lower epidermis of the leaf. The second multispectral detector is located on the optical axis of the excitation light source. The three multispectral detectors are arranged to obtain the reflection, transmission and absorption spectra of the leaf, and the physiological index is inferred by coupling with environmental factors, so that the plant health state can be monitored in real time in the scenes of precision agriculture, ecological monitoring and the like.

The invention relates to the technical field of plant physiology and environment simulation, and discloses a plant photosynthesis CO2 concentration multiplication and isotope labeling device and an experimental method.The gas inlet system comprises a gas inlet connector, a pressure reducing valve, a diffusion pipeline and a direct gas inlet pipeline; the diffusion pipeline is arranged along the inner wall surface of the transparent box body, extends to the top area of the transparent box body and then extends to the middle of the transparent box body, air holes are formed in the pipe wall of the diffusion pipeline, and the direct air inlet pipeline extends to the lower area of the transparent box body; the stirring and mixing system comprises at least one stirring fan arranged on the side wall of the transparent box body; the monitoring system comprises an air pressure monitoring device and a gas concentration detection device which are arranged on the side wall of the transparent box body, and the gas concentration detection device is integrated with a CO2 channel probe, an O2 channel probe and an N2 channel probe and is used for synchronously monitoring the gas concentration in the transparent box body. The device can be widely applied to plant photosynthesis response research and carbon distribution path tracing experiments.

The utility model relates to the field of plant physiology, in particular to a green plant light supplementing cultivation planting frame which comprises an installation frame, planting openings are evenly distributed in a front-back symmetrical mode, the front portion and the rear portion of the installation frame are each connected with two outer frames in a left-right symmetrical mode, and connecting pieces are connected in the outer frames in a sliding mode. A plurality of hole grooves used for positioning are formed in the connecting pieces, arc-shaped sliding grooves are formed in the upper portions of the connecting pieces, first clamping bolts are connected to the outer frames in a sliding mode, first springs are connected between the first clamping bolts and the adjacent outer frames, the first springs are arranged on the first clamping bolts in a sleeving mode, and the second clamping bolts are connected with the first springs in a sleeving mode. The inner side of each first clamping bolt is of an inclined face structure. The height of the light supplementing lamp is flexibly adjusted through cooperation of the sliding connecting piece and the light supplementing plate, meanwhile, in the growth process of green plants, the connecting piece of a fan-shaped structure is used in cooperation with the arc-shaped sliding groove and the second clamping bolt, the uniformity of illumination is optimized, and the light supplementing lamp is more suitable for the requirements of the plants in different growth stages.

The application relates to application of a miR531 gene of Dongnong winter wheat No.1 in regulation of plant growth and cold resistance and cultivation of cold-resistant plants and belongs to the technical field of plant physiology and molecular biology, characterized in that the nucleotide sequence of the miR531 gene found from Dongnong winter wheat No.1 is shown in Seq ID No:1. After the miR531 gene is connected to an overexpression vector pCambia230035Su, the miR531 gene can be integrated into a plant genome through agrobacterium infection. The miR531 transgenic Arabidopsis obtained through the transgenic means shows that the number of rosette leaves and branches is significantly increased, and the cold tolerance is enhanced. Therefore, the miR531 gene found from Dongnong winter wheat No.1 has the functions of improving plant growth and enhancing cold resistance. The application is simple in production, high in operability, low in cost and obvious in effect.

The present application relates to the technical fields of environmental science and plant physiology, and discloses a kind of temperature increasing equipment for controlling plant illumination, comprising temperature increasing cover, illumination simulation mechanism, light blocking mechanism and auxiliary temperature increasing mechanism, wherein: the temperature increasing cover is transparent conical cylinder, and the shell of the temperature increasing cover is provided with a cavity, and the inner wall of the temperature increasing cover is distributed with several air holes connected to the cavity.Light simulation mechanism is arranged above the opening at the top of the temperature increasing cover.The light blocking mechanism is arranged around the temperature increasing cover, which can be folded and stored.The auxiliary temperature increasing mechanism is provided with several and distributed on the outside of the temperature increasing cover, and the auxiliary temperature increasing mechanism is used to input heat into the cavity.The present application can simulate the plant growth environment with the same illumination and temperature as much as possible at different monitoring points outdoors, so as to obtain more accurate experimental results.

The application discloses a kind of genes RhNADP-LIKE for regulating the aging of Chinese rose flower, and specifically relates to the technical field of molecular biology and horticultural plant physiology.The application filters the bottle insertion life phenotype data of 358 tetraploid Chinese rose core germplasm by whole genome association analysis (GWAS), and identifies the gene RhNADP-LIKE significantly associated with aging.The gene accelerates the petal aging process by regulating the accumulation of reactive oxygen species (ROS).After inhibiting the expression of RhNADP-LIKE using virus-induced gene silencing (VIGS) technology, the opening period of Chinese rose flower is extended by about 2 days, and the ROS level is significantly reduced.The application provides a molecular target for extending the bottle insertion life of Chinese rose, which can be applied to gene editing breeding or the development of environmentally friendly preservatives, reducing the dependence on chemical agents, and improving the economic benefits and sustainable development potential of cut flower industry.

The invention discloses a young ear continuous induction method for tissue culture of Pennisetum americanum, and relates to the related fields of plant physiology and molecular biology. The method comprises the following steps: (1) planting American pennisetum seedlings in limited nutrient soil; (2) preparing a large container filled with sufficient nutrient soil; (3) after the pennisetum americana enters a reproductive growth period and stem sections of main stems for breeding young ears are obtained, the small square pots with seedlings are placed in a large container with sufficient nutrition; and (4) collecting newly extracted lateral bud stems once every 3-4 days. The method has the following advantages and positive effects: (1) the plant height of the pennisetum americanum can be controlled, and young ears can be conveniently picked; (2) stem sections for breeding young ears can be produced after single planting for about 3 months; and (3) the stem sections of the inoculated young ears can be quickly collected in batches at a time, so that the time required for judging the development stage of the young ears is greatly shortened, the young ears for generating embryogenic calluses are obtained, the acquisition of transgenic materials is facilitated, and the verification of gene functions of the pennisetum americanum is accelerated.