120 results about "Plant physiology" patented technology

The invention discloses a plant physiology monitoring method based on chlorophyll fluorescence and a multispectral image. The method comprises the following steps that modulated type chlorophyll fluorescence detection is performed on plants to be detected, modulated type chlorophyll fluorescence characteristic parameter diagrams are acquired, and chlorophyll fluorescence parameters related to the plant physiology condition are preset according to the characteristic parameter diagrams; multispectral image measurement is performed on the plants to be detected, and a spectral relative reflectivity parameter diagram of the reflection intensity at the waveband of 540-560 nm to the reflection intensity at the waveband of 500-515 nm is obtained; chlorophyll fluorescence parameter values in a plant area and the spectral relative reflectivity parameter diagram are counted; the plant physiology condition is judged by combining a distribution curve, acquired in the step 3, of the chlorophyll fluorescence parameter values and a distribution curve, acquired in the step 3, of spectral reflectivity parameter values. According to the method, nondestructive testing is performed on the plant physiology by combining a multispectral imaging technology of visible light with a chlorophyll fluorescence detection technology, the method is simple, judging is accurate, and the reliability is high.

The invention relates to the field of plant physiology, in particular to a method for screening saline-alkaline resistant/tolerant cotton. The method comprises the steps that (1), a cotton culture solution is prepared, and the pH value of the cotton culture solution is adjusted between 6.5 and 7.0; (2), the culture solution serves as a solution to prepare NaCl solutions with the concentration being 0.0 wt%, 0.1 wt%, 0.2 wt%, 0.3 wt%, 0.4 wt%, 0.5 wt%, 0.6 wt% and 0.8 wt%; (3), a sand sprout method is adopted, when cotton seeds sprout and grow until all cotyledons are spread and turn into green and no root is generated, the cotton seeds are moved in the NaCl solutions; (4), cultivation is carried out in a shading condition; (5), in the ninth to tenth day, seedlings are taken out for surveying the root hair number of each cotton seedling, and the saline-alkaline resistant/tolerant degree of cotton is judged according to the number of the root hairs. The method excludes the interference of the factors that salinity gradient is uneven and rainfall wets salt in the field identification process, the duration is short, the identification result is stable and reliable, and method is high in practicability and maneuverability.

The invention discloses a sampling method for non-damage fixed position observation of plant stomata in a field experiment, which comprises the following steps of: according to experimental design, selecting functional leaves, avoiding overripe leaves, over tender leaves, leaves damaged by diseases and insects and malformed leaves, and cleaning object leaves; evenly coating a thin layer of nail polish at a position to be observed, and natural drying or blowing to be nearly dry by using a washing ear ball; flatly and tightly pasting a transparent adhesive tape on a clean glass slide, using a dissecting needle or a clean blade for slowly guiding to prevent air bubbles, and tightly pasting the adhesive tape with the width being at least 2mm around the nail polish on the glass slide; and numbering, clean packaging, and taking back to the laboratory for microscopic observation and measurement. The method has the advantages of being capable of bulk sampling in field living leaves, having no damage to the leaves, fixed position recording stomatal conditions, observing daily change of the stomata of the leaves under different processing conditions, and the like, and can be used for the research and practice in the fields such as bioecology, plant physiology, plant germplasm resource, plant thremmatology, cultivation science, etc.

The present invention generally relates to the regulation of plant physiology, in particular to methods for inhibiting the ethylene response in plants or plant products. First,the invention relates to methods of minimizing impurities capable of reversibly binding to plant ethylene receptor sites during the synthesis of cyclopropene and its derivatives such as methylcyclopropene, thereby avoiding the negative effects these impurities have on plants treated with cyclopropene and its derivatives. Second, the invention relates to complexes formed from molecular encapsulation agents such as cyclodextrin, and cyclopropene and its derivatives such as methylcyclopropene, in addition to cyclopentadiene and diazocyclopentadiene and their derivatives, thereby providing a convenient means for storing and transporting these compounds capable of inhibiting the ethylene response in plants, which are reactive gases and highly unstable because of oxidation and other potential reactions. Thirdly, the invention relates to convenient methods of delivering to plants these compounds capable of inhibiting the ethylene response in the plants in order to extend their shelf life.

The invention belongs to the field of plant physiology, and discloses a method for extracting sucrose synthase and sucrose phosphate synthase of pear fruits and an activity determination method thereof. Small molecules such as sugar, ions and the like can be usually removed from a primary extraction enzyme solution through adding a salting-out step and a dialysis step in the extracting process of the sucrose synthase and the sucrose phosphate synthase, so that activities of the sucrose synthase and the sucrose phosphate synthase in the fruits can be accurately detected. The method provides physiological basis for disclosing accumulation differences of the sugar in the fruits, and further provides theoretical basis for improving the quality of fruit sweetness and flavor.

The ion exchange and reversed phase chromatographic separation process for extracting abscisic acid from fermented liquid includes the following steps: the first macroporous resin adsorption of abscisic acid from fermented liquid to extract and concentrate abscisic acid in fermented liquid initially; the subsequent silica gel chromatography to eliminate much of fermentation side products and obtain natural abscisic acid product with relatively high purity (higher than 90 %); and final C18 silica gel reversed phase chromatographic separation to obtain natural abscisic acid product with purity higher than 98 %. The process can obtain high purity abscisic acid product suitable for plant physiology research.

The invention discloses a method for improving germination of stevia rebaudiana seeds, which relates to a crop seed treatment technique in the technical field of agriculture. The method comprises the following steps of: selecting and sterilizing seeds, preparing 6 to 8 percent aqueous solution of polyethylene glycol 4000, soaking the seeds into the prepared solution at the temperature of between 20 and 30 DEG C for 24 to 48 hours, then filtering the seeds, cleaning the seeds by using clear water, and airing the seeds for later sowing. In the method, the used polyethylene glycol is generally recognized that molecules of the polyethylene glycol can change biological membrane structures of various cells in cell engineering so that lipid molecules on a plasma membrane at a contact point of two cells are dispersed and recombined, can change the osmotic regulation capability of plants on plant physiology and are favorable for absorbing nutrition and inducing the activity of stimulation cells. The method can remarkably improve the capability of resisting adverse situations when the stevia rebaudiana seeds are germinated so that the stevia rebaudiana seeds still maintain higher germination rate and germination energy in adverse situation harm and the seedlings have good quality.

Provided are methods for increasing seed size and/or weight, embryo size and/or weight, and cotyledon size and/or weight. The methods comprise expression of a plant cytokinin oxydase.

The invention provides an organic rice planting method. The organic rice planting method comprises the following steps of S1, precision sowing, wherein sowing time selection, variety selection, seed sun-curing, seed soaking, germination accelerating and bud airing are conducted; S2, sowing and seedling cultivation, wherein sowing quantity judgement, seeding tray preparation, seeding tray seeding quantity determination, seedling cultivation substrate preparation, seedling cultivation site preparation, seedbed preparation, planosol filling, sowing and seedbed management are conducted; S3, material and production preparation before rice transplanting, wherein selection of organic fertilizer and cultivation of crops for expelling and killing pests are conducted; S4, rice planting with machines, wherein rice transplanting seedling age judgement, fertilizer preparation, land preparation, fertilizer application, rice transplanting rote designing, rice transplanting machine selection, rice transplanting time determination, seedling taking quantity adjustment and rice transplanting density and depth determination are conducted; S5, field management, wherein gaps are filled with the seedlings, secondary water management, base fertilizer application and top application are conducted, and disease and pest prevention and control technologies and grass damage prevention and control technologies are adopted. By combining and applying theories of pesticide science, cultivation science, soil science, fertilizer science, plant physiology and field ecology, the organic planting method on thebasis of traditional Chinese medicinal herb preparations is formed.

The invention relates to a water-soluble calcium-magnesium fertilizer which is prepared from the following raw materials in parts by weight: a calcium-containing raw material, a magnesium-containing raw material, an organic fertilizer, an absorption accelerant, trace elements and water. The water-soluble calcium-magnesium fertilizer provided by the invention comprises completely water-soluble calcium-magnesium elements, can be absorbed and utilized by crops and meanwhile has an appropriate calcium and magnesium ratio; due to addition of the organic fertilizer, the absorption accelerant and thetrace elements, the calcium-magnesium elements in the fertilizer meet synergetic absorption of the calcium-magnesium elements in plant physiology and are promoted by one another to take the effects into play, and together with the organic fertilizer, the absorption accelerant and the trace elements, growth of crops can be promoted, and the yield of the crops can be remarkably increased.

The invention provides a forest and fruit base growth substance for cold regions and arid regions. The forest and fruit base growth substance is composed of the following components in percentage by weight: 20-40% of urea, 10-30% of potassium hydrogen phosphate, 8-20% of carrageenan powder, 25-55% of starch, 5-15% of dry yeast powder and the balance of brown sugar powder. In early spring, by virtue of the characteristic that the surface soil is thawed and the deep soil is not thawed, the forest and fruit base growth substance solution provided by the invention is uniformly input beneath the shallow surface soil layer, to promote quick temperature rise of the surface soil layer, activate soil microorganisms, reinforce first recovery of shallow root rates of a plant and obtain sufficient supply of water and fertilizer, so as to drive the earlier recovery of the entire plant, conform to the principle of plant physiology, collect light and heat resources in advance, prolong the photosynthetic reaction time of leaves and generate more biomass.

The invention relates to a plant characteristic spectrum experimental box and an experimental method thereof. A plurality of monocolor LED (Light-Emitting Diode) light sources, a multi-layer culturing chamber, a temperature regulating device, a humidity regulating device, an intelligent control device and the like are arranged in the plant characteristic spectrum experimental box, the plant characteristic spectrum experimental method comprises the steps of irradiating leaves of the plant respectively by adopting different wavelengths of monocolor light, measuring corresponding light intensity by adopting a spectrum radiometer, testing the photosynthesis rate of leaves by adopting a photosynthesis tester to obtain the relationship of the light intensity corresponding to corresponding wavelength and the plant photosynthesis rate, thus obtaining the characteristic spectrum of the specific plant; and carrying out cultivation experiments by taking the obtained plant characteristic spectrum as a lighting source for the plant, thus obtaining the relationship of lighting time and photosynthesis of the light source and the yield. The plant characteristic spectrum experimental box and the experimental method thereof provide experimental technology and means for researching plant physiology, and lay the foundation of manufacturing of characteristic spectrum light source suitable for plant growth, thus being capable of meeting the demand on the scientific and research and production of modern agriculture.

The invention discloses a method for releasing dormancy of pear seeds, relates to imbibition, whitening and germination of seeds, and belongs to the fields of plant physiology and production cultivation. According to the method disclosed by the invention, seeds are soaked in an earlier stage; successively, the seeds are coated by degreasing cotton on seed coat; the features of good moisture retention performance and good permeability of the degreasing cotton are utilized, the dormancy of the pear seeds is completely broken through cold stratification. The method has the advantages of easiness for operation, simple steps, convenience for observation and the like, and has a good effect on breaking dormancy; moreover, by adopting the degreasing cotton for coating, chemical agent and hormones are conveniently added so as to research the effect on release of seed dormancy.

The present invention relates to methods for stimulating root growth and/or enhancing the formation of lateral or adventitious roots and/or altering root geotropism comprising expression of a plant cytokinin oxidase or comprising expression of another protein that reduces the level of active cytokinins in plants or plant parts. Also provided by the present invention are methods for increasing seed size and/or weight, embryo size and/or weight, and cotyledon size and/or weight. The methods comprise expression of a plant cytokinin oxidase or expression of another protein that reduces the level of active cytokinins in plants or plant parts. The invention also relates to novel plant cytokinin oxidase proteins, nucleic acid sequences encoding cytokinin oxidase proteins as well as to vectors, host cells, transgenic cells and plants comprising said sequences. The invention also relates to the use of said sequences for improving root-related characteristics including increasing yield and/or enhancing early vigor and/or modifying root/shoot ratio and/or improving resistance to lodging and/or increasing drought tolerance and/or promoting in vitro propagation of explants and/or modifying cell fate and/or plant development and/or plant morphology and/or plant biochemistry and/or plant physiology. The invention also relates to the use of said sequences in the above-mentioned methods. The invention also relates to methods for identifying and obtaining proteins and compounds interacting with cytokinin oxidase proteins. The invention also relates to the use of said compounds as a plant growth regulator or herbicide.

The invention relates to plant protein extracting, in particular to a non-degeneration plant holoprotein extracting solution and a preparing method thereof. The extracting solution is characterized by being an aqueous solution containing a phosphate buffer solution, EDTA, glycerol, PVP and protease inhibitors. The extracting solution has the advantages that plant holoprotein extracted with the holoprotein extracting solution is suitable for analyzing the activity of protease and measuring the protein concentration with methods such as BCA; the holoprotein is not degenerated, reducing agents influencing measuring of the BCA and the like are not added, the extracted holoprotein can be stored for several months in the frozen mode, and sediment is basically avoided after re-melting; in addition, the activity of the holoprotein is basically free of influences before and after storage, the extracted holoprotein is high in amount and can be further directly used for further separation purification, and therefore the extracting solution has important application value in the aspect of plant physiology and biochemistry researching.

The invention discloses an edible vegetable oil bactericide and an application method thereof. The bactericide contains the following raw materials according to the volume percent: edible vegetable oil of 80% -95%, and emulsifier of 5% -20%; the emulsifier is emulsifier EL or polyethenoxy ether emulsifier. The application method of the bactericide is that the edible vegetable oil and the emulsifier is fully stirred, blended and then diluted with water for insufflation. The edible vegetable oil bactericide has good stability as farm chemical missible oil, and good control efficiency on powdery mildew of cucumber and other powdery mildew, with effective rate of 60%-75%, which is equivalent to that of triazolone missible oil diluted 1000 times; furthermore, the bactericide has no influence and hazard on plant physiology and is an ideal bactericide which is eco-friendly, with the sterilization effect satisfying the ecological requirements, and is suitable for the hazard-free production of vegetables..

The invention belongs to the plant root spatial distribution technical field and relates to a method and system for measuring the spatial distribution of a plant root system. According to the method and system of the invention, image segmentation is performed on the video data of on original root system, a spatial scope to which original plant root system skeleton points belong is determined by using a voting principle; root system points are detected in images through the voting principle, the vertical ordinate of the center of an ellipse is calculated through using an ellipse equation; the positions of the original root system in different horizontal layers of a growth medium are determined according to the value of the vertical ordinate of the center of the ellipse; and the positions ofthe root system in different horizontal regions of the growth medium are determined according to the direction of the end coordinates of the semi-major axis of the ellipse. According to the method and system of the invention, the ratio data of the original root system are obtained; and the three-dimensional parameters of the form and configuration of the plant root system can be quantified by using the ratio data of the original root system, and in-situ and dynamic observation can be performed by using the ratio data. Conveniences can be provided for the scientific research of subject fieldssuch as root biology, plant nutrition, plant physiology and plant growth computer simulation.

The invention discloses a method for predicting the suitable habitat of a ginkgo fruit forest based on climate and soil factors, and belongs to the technical field of species distribution prediction. The method comprises the steps of firstly, establishing a climate ecological niche model and a soil ecological niche model; predicting the habitat types of the ginkgo fruit forest by using the climate ecological niche model and the soil ecological niche model, and classifying the habitat types of the ginkgo fruit forest; carrying out verification test on the model and a prediction result through a seed field test; filtering the climate-suitable habitat by using the soil-suitable habitat; and predicting the change of the suitable habitat of the ginkgo fruit forest under the future climate scene. The two models are combined, the current and future under different climate change scenes can be predicted, the reliability of model output is verified through experimental data, the prediction process is more efficient, the result is more accurate, prediction of the habitat suitability classification model conforms to the knowledge of phytophysiology and economics, and the habitat suitability classification model has good application prospects. The method has important significance for guiding the production practice of the ginkgo fruit forest.

The invention provides a wetland ecological information system which stores wetland ecological data in a database, wherein the database comprises an interface-connected plant landscape information unit, a vector gate information interaction unit, a plant physiological information unit, an animal habitat information unit, an animal habitat forecasting unit and data acquiring equipment. The plant landscape information unit is used for forming into plant landscape thematic information in a classifying way; the vector gate information interaction unit is used for obtaining the vector gate interaction information of different times, different types and different data sources of the wetland; the plant physiological information unit is used for forming into plant physiology thematic information; the animal habitat information unit is used for forming into animal habitat thematic information; the animal habitat forecasting unit is used for forming into animal habitat variation tendency thematic information; and the information is also stored in the database and is outputted as well as displayed through output equipment.

The invention provides a plant tissue culture bottle washing device applied to experiments of plant physiology. The plant tissue culture bottle washing device comprises a washing device body, whereinthe washing device body comprises a washing tank and an arrangement frame; a partition plate is fixed at a position close to the bottom end of the washing tank. When the plant tissue culture bottle washing device is used for washing culture bottles, the bottom ends of the culture bottles are placed into fixing cylinders; guide plates of a clamping plate are aligned to guide sliding grooves, and athrough hole is aligned to a fixing column; clamping holes are sleeved on openings of the culture bottles at the moment, then the clamping plate is pressed, and a fixing nut is sleeved and is used forfixing the culture bottles; the arrangement frame is inverted on a placement frame of the washing tank; washing brushes stretch into the culture bottles at the moment, then a motor is started, a rowof driving gears is driven to rotate when the motor rotates; the driving gears transmit rotation to each row of driven gears at the tail ends; then the each row of driven gears at the tail ends transmit rotation to the row of driven gears, so that all the driven gears rotate together to wash the culture bottles.