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Amorpha fruticosa is a species of flowering plant in the legume family (Fabaceae) known by several common names, including desert false indigo, false indigo-bush, and bastard indigobush. It is found wild in most of the contiguous United States, southeastern Canada, and northern Mexico, but it is probably naturalized in the northeastern and northwestern portion of its current range. The species is also present as an introduced species in Europe, Asia, and other continents. It is often cultivated as an ornamental plant, and some wild populations may be descended from garden escapes.
Proteins having tetrahydrofolate-dependent demethylase activity and their use in l-5-methyltetrahydrofolate synthesis
PendingCN122303180AAmorpha fruticosaDihydrofolic acid
This invention belongs to the field of enzyme engineering technology, specifically relating to proteins with tetrahydrofolate-dependent demethylase activity and their application in the synthesis of L-5-methyltetrahydrofolate. This invention utilizes two proteins with tetrahydrofolate-dependent demethylase activity, namely *Sphingosine monocytogenes* (…). Sphingomonas paucimobilis Vanillic acid O-demethylase and Amorpha fruticosa intermediate rhizobia (from) Mesorhizobium amorphae The aminomethyltransferase derived from 6S-THF uses inexpensive vanillic acid or dicamba as methyl donors to directly methylate 6S-THF to synthesize L-5-MTHF. Combined with highly active dihydrofolate reductase and NADP cofactor regeneration system, this method effectively achieves efficient and green synthesis of L-5-methyltetrahydrofolate, and has good industrialization prospects.
Owner:SHANDONG UNIV