66 results about "Vanillic acid" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to pseudomonas monteilii BDLZ-3 and application thereof in relieving continuous cropping obstacles of crops. The invention discloses a strain of pseudomonas monteilii BDLZ-3, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.34349. The pseudomonas monteilii BDLZ-3 is separated and screened from the nature for the first time, phenolic acid autotoxic substances generated in the ginseng continuous cropping obstacle process can be efficiently degraded, the degradation rate of benzoic acid can reach 95.36%, various phenolic acids such as vanillic acid, cinnamic acid, palmitic acid and gallic acid can be effectively degraded, and the degradation spectrum is wide; when the pseudomonas monteilii BDLZ-3 is applied in a field, the content of benzoic acid in soil can be effectively reduced, meanwhile, the occurrence of ginseng root diseases can be controlled to a certain extent, the prevention and treatment effect reaches 41.23%, the growth indexes such as root length and root thickness are remarkably improved, and the yield increasing effect is achieved.

The invention relates to the technical field of agricultural biological prevention and control, and discloses a biological prevention and control complex microbial inoculant and application thereof.The biological prevention and control complex microbial inoculant is prepared by the steps that natural bio-based compounds vanillin and vanillic acid serve as main raw materials, and a biodegradable trialdehyde monomer is designed and synthesized; a biodegradable trialdehyde monomer and p-phenylenediamine are used as organic building units to assemble and form an ordered porous crystal structure, and the biodegradable COF material is obtained; the preparation method comprises the following steps: loading a basic complex microbial inoculant consisting of bacillus thuringiensis, bacillus subtilis and streptomyces willerigensis on a biodegradable COF material by adopting an impregnation adsorption method, and coating the surface of the biodegradable COF material with tetraethylenepentamine and tannic acid through copolymerization to form a degradable polytannic acid layer, so as to prepare the slow-release complex microbial inoculant, and the effective active components of the slow-release complex microbial inoculant can be slowly released; the lasting period and the utilization rate of the composite microbial agent can be remarkably improved, and the composite microbial agent shows an obvious inhibition effect on pathogenic bacteria such as fusarium oxysporum and botrytis cinerea and can be applied to prevention and treatment of plant diseases.

The invention belongs to the technical field of soil improvement, and discloses a preparation method of a composite microbial agent and application of the composite microbial agent in continuous cropping stress resistance, the active ingredients of the composite microbial agent comprise Gliocladium mucilaginosum, Trichoderma harzianum and Pseudomonas chlororaphis, and a carrier contains a basic carrier, a synergistic component and a soil adaptive component (oyster shell powder or wormcast particles). The preparation method comprises the following steps: strain fermentation, carrier construction, solid-state co-fermentation, and mixing of a dried fermentation product and a carrier. After the compound microbial agent is used, the abundance of gliocladium mucilaginosum, trichoderma harzianum and pseudomonas chlororaphis in continuous cropping crop soil is obviously increased, and the abundance of fusarium oxysporum is obviously reduced; the acidification condition of the acidified soil is obviously improved, the content of organic acids (ferulic acid and vanillic acid) in the soil is obviously reduced, and the soil becomes loose from hardening, so that the root activity is improved, and the plant diseases and insect pests are resisted; the single fruit weight and sugar degree can be remarkably improved, the fruit storage rotting rate is reduced, and the effect is remarkable.

The invention belongs to the technical field of medical analysis, and particularly relates to a method for separating chemical components in hemsley rockvine root. According to the present invention, various chromatography methods are adopted to carry out separation and purification, such that sitroside (I), vanillyl glycolII (II), 5-hydroxymaltol (III), vanillic acid (IV), afzelin (V), 2, 3-dihydroxypyrazole (9E, 11E)-13-hydroxypyrazole (9, 11-dienoate), and 2, 3-dihydroxypyrazole (10E, 12E)-9-hydroxypyrazole (10, 12-dienoate) are obtained from hemsley rockvine root. Wherein the compounds II, III, VI and VII are separated from the hemsley rockvine root for the first time, and development and quality control of the hemsley rockvine root medicinal material are facilitated.

The invention provides a novel application of a raspberry extract. It is found through tests that any one of isoquercitrin, ellagic acid, vanillic acid, oleanolic acid, kaempferol-3-O-rutinoside, kaempferol-3-O-sophoroside, tiliroside, tormentic acid and raspberry extract can remarkably improve the activity of the human ovarian granular cells or remarkably improve the oxidative stress level, and the activity of the human ovarian granular cells can be remarkably improved. According to the present invention, the ovarian function can be effectively protected by the eight components, the ovarian function can be effectively protected by the eight components, or the mitochondrial membrane potential level can be significantly regulated and controlled, or the estrogen level in the microenvironment can be effectively maintained, such that the ovarian function can be effectively protected by the eight components, and the raspberry extract containing the eight components. The invention provides a new choice for ovarian function protection products, and also provides a new application for the substances.

The invention relates to the technical field of catalysts, in particular to a preparation method of an iron-cobalt oxide and Beta molecular sieve composite catalyst and application of the iron-cobalt oxide and Beta molecular sieve composite catalyst to demethoxylation of lignin derivative vanillic acid. Meanwhile, due to the interaction of iron and cobalt species, the acid site of the catalyst is enhanced, and adsorption and activation of a substrate are facilitated; the iron-cobalt oxide is combined with the Beta molecular sieve, and the reaction activity of demethoxylation is improved through cooperation of metal / zeolite; the Co0 / Fe0. 04Co0. 96O-Beta composite catalyst is used for a vanillic acid demethoxylation catalytic reaction, the reaction is performed for 2 hours under the condition of 0.4 MPa H2 and 0.4 MPa CO2, the substrate conversion rate reaches 96.07%, the p-hydroxybenzoic acid selectivity reaches 86.96%, and the target product yield is 83.54%.

The application provides a pharmaceutical composition for functional dyspepsia and a preparation method and application thereof, and belongs to the technical field of medicines, and the pharmaceutical composition comprises the following components: Aucklandia, Atractylodes, Mentha, Agastache, Citrus aurantium, Uncaria, Tribulus terrestris, Guangdong Shenqu, Chrysanthemum, Poria cocos, Radix Trichosanthis, Coix seed, Magnolol, Imperatorin, Isoimperatorin, Oxypeucedanin, Ferulic acid, Vanillic acid, p-Coumaric acid and Puerarin. The pharmaceutical composition comprises traditional Chinese medicine components and compounds, the components interact with each other, the effect of the pharmaceutical composition on improving functional dyspepsia can be improved, and meanwhile, the pharmaceutical composition has excellent stability.

The present invention relates to a vanillic acid derivative compound which can be formed by a dehydration condensation reaction between vanillic acid and catecholamine. The vanillic acid derivative compound according to the present invention is characterized in that it can be used as a therapeutic preparation for Alzheimer's disease and neuroinflammation. In addition, the present invention relates to a therapeutic composition for Alzheimer's disease and a therapeutic composition for neuroinflammation, each of which comprises the compound.

The invention relates to a carboxylic acid reductase (MACAR) from Mycobacterium agri, and the carboxylic acid reductase (MACAR) shows higher substrate conversion efficiency than that of a source NiCAR (NiCAR) from Nocardia iowensis, and is suitable for the conversion production of vanillin from vanillic acid. A systematic process for efficient screening and verification of carboxylate reductase CAR identifies a plurality of novel CARs with potential industrial application values, and lays a foundation for directed evolution and industrial application.

This invention relates to a polyester polyol, a polyurethane resin, their preparation methods, and applications, belonging to the field of synthetic leather technology. The raw materials for preparing the polyester polyol, by weight percentage, include 7-15% polyethylene glycol, 22-39% p-hydroxybenzoic acid, 31-46% vanillic acid, and 12-24% 1,3-propanediol. The raw materials for preparing the polyurethane resin, by weight percentage, include 4-20% of the above-mentioned polyester polyol, 3-19% aliphatic polycarbonate diol, 1-4% isocyanate A, 1-4% isocyanate B, 1-4% aromatic polyester diol, and 60-80% solvent A. This invention, through formulation design of the polyester polyol and polyurethane resin, produces synthetic leather with high blackness and high gloss, which can maintain this high blackness and high gloss effect for a long time.

The invention belongs to the technical field of medicinal chemistry, and particularly relates to a dasatinib vanillic acid crystal form and a preparation method thereof, and in the dasatinib vanillic acid crystal form provided by the invention, the molar ratio of dasatinib to vanillic acid to water is 1: 1: 1. The dasatinib vanillic acid crystal form provided by the invention has relatively good stability and relatively high dissolution rate, and is of great significance for improving the bioavailability of a dasatinib medicine and preparing a preparation.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to a minocycline hydrochloride-melatonin compound colon targeting capsule and a preparation method thereof. The capsule comprises a pellet and a capsule shell, wherein the pellet comprises a medicine core and a colon-targeted sustained-release layer wrapping the medicine core. The medicine core comprises active ingredients of minocycline hydrochloride and melatonin; the colon-targeted sustained-release layer comprises a special pH-enzyme colon-targeted response agent and a self-made plasticizer. The pH-enzyme responsive agent is based on a hydroxypropyl methylcellulose grafted vanillin derivative, and can be degraded in response to pH change and azo reductase at the colon part; the plasticizer has good compatibility with a coating material, and can effectively reduce leakage of drugs at the front ends of the stomach and intestines. The capsule prepared by the invention can obviously improve gastrointestinal stimulation of minocycline hydrochloride, realizes targeted release of the medicine at the colon part, and shows an excellent treatment effect on ulcerative colitis while reducing the dosage of antibiotics by utilizing the synergistic effect of melatonin.

The purpose of the present invention is to provide a microorganism which can selectively produce and / or accumulate vanillic acid while growing with use of not only H-type lignin-derived aromatic compounds but also S-type lignin-derived aromatic compounds as carbon sources, and a method for using the microorganism to produce vanillic acid. The purpose is achieved by, for example: a transformed microorganism, the host microorganism of which is Pseudomonas species (Pseudomonas sp.) NGC7 strain (accession number: NITE BP-03043), and in which a vanA4 gene (SEQ ID NO: 1) or the vanA4 gene and a vanB4 gene (SEQ ID NO: 2) on the chromosome are deleted; and a method for using the transformed microorganism to produce vanillic acid.

The invention discloses an L-carnitine-based ionic liquid aqueous two-phase system as well as a preparation method and application thereof, and belongs to the technical field of aqueous two-phase system separation. The prepared L-carnitine-based ionic liquid aqueous two-phase system is a novel ionic liquid aqueous two-phase system which is more efficient when active substances are extracted (extraction and separation can be achieved by stirring for 30 min and centrifuging for 5-8 min at the speed of 6000-8000 rpm), the extraction rate of vanillin, eugenol, p-hydroxybenzaldehyde, syringaldehyde and vanillic acid reaches 85.37% or above, and the L-carnitine-based ionic liquid aqueous two-phase system is a novel ionic liquid aqueous two-phase system. The method can be applied to the fields of organic compound extraction, medicine extraction, environmental protection and the like, and has a great development prospect.

The present invention relates to use of a compound represented by formula (I) or an ester thereof or a pharmaceutically acceptable salt thereof in the treatment of a disease accompanied by fibrosis.

The application discloses vanillyl octyl ester, a preparation method and application thereof, and relates to the field of insulating oil additives. Vanillyl acid and n-octanol are subjected to esterification under the action of a biological enzyme catalyst to synthesize vanillyl octyl ester, and the synthesis method is efficient, green, environmentally friendly and low in cost. The prepared vanillyl octyl ester is added into synthetic ester insulating oil as an antioxidant active ingredient, the long ester chain in the vanillyl octyl ester has high thermal stability, can effectively inhibit the oxidation of the synthetic ester insulating oil at high temperature, and has good compatibility with the synthetic ester insulating oil. Therefore, compared with traditional antioxidants, the vanillyl octyl ester has significant advantages in maintaining excellent antioxidant properties and electrical properties of the synthetic ester insulating oil.

The invention discloses an upatinib eutectic crystal form as well as a preparation method and application thereof, and belongs to the technical field of medicinal chemistry. The invention provides an eutectic crystal form I formed by upatinib and vanillic acid and an eutectic crystal form G formed by upatinib and syringic acid, which have definite and reproducible characteristic X-ray powder diffraction pattern and melting point, have excellent solubility in an aqueous medium, do not generate crystal form transformation after being stored for 1 month under the condition of relative humidity of 40 DEG C / 75%, and can be used for preparing a crystal form of upatinib. And the stability is good. The preparation process route is short, the operation condition is mild, the used solvent is conventional, the target eutectic can be efficiently and repeatedly obtained, the defects that some existing eutectic preparation processes are complex, long in period or harsh in condition are overcome, and the method has good process amplification and industrialization prospects.

The present invention provides a hair coating composition comprising: a first polyphenol including at least two among tannic acid, gallic acid, and caffeic acid; and a second polyphenol comprising at least one selected from the group consisting of a biphenyl moiety, dopamine, catechin, anthocyanin, resveratrol, quercetin, protocatechuic acid, p-hydroxybenzoic acid, vanillic acid, ferulic acid, luteolinidin, apigeninidin, and thureolin, wherein the composition can restore and improve the mechanical strength of hair through a Michael addition reaction with a thiol group included in damaged hair.

The application provides a kind of pumpkin stem medicinal materials, decoction piece, standard decoction and its formula granule HPLC content determination construction method, comprising: A) pumpkin stem raw material is extracted using solvent, to obtain the test solution;Vanillic acid control sample is dissolved using 70% ethanol, to obtain reference solution;B) the test solution and reference solution are determined using high performance liquid chromatography, to obtain the chromatogram of test solution and reference solution respectively;And according to the chromatogram of reference, the components of pumpkin stem medicinal materials, decoction piece, standard decoction and its formula granule HPLC content determination are qualitatively and quantitatively determined;The high performance liquid chromatography chromatographic conditions are: the chromatographic column is C18 column;The mobile phase is methanol-0.2% acetic acid.The application uses high performance liquid chromatography, selects methanol-0.2% acetic acid as mobile phase for elution, determines the content of vanillic acid in pumpkin stem medicinal materials, decoction piece, standard decoction and its formula granule HPLC, and has good repeatability, precision, stable and reliable method.

The application relates to the technical field of packaging printing ink, and discloses a packaging printing ink capable of being cured by ultraviolet light and a production process thereof, the packaging printing ink comprises additives such as wear-resistant fillers and antioxidant fillers, wherein the wear-resistant fillers are prepared by grafting vinyl ester resin on the surface of nano-hydroxyapatite, the vinyl ester resin can improve the compatibility of the nano-hydroxyapatite and a base material, can directly bear and disperse part of pressure and shear force, avoids stress concentration on the base material, reduces a friction coefficient, thereby reducing wear, meanwhile, the vinyl ester resin has good toughness, and can improve the folding resistance of an ink film; the antioxidant fillers are prepared by introducing vanillic acid into butyronitrile rubber with alkenyl groups, the vanillic acid has antioxidant property, the alkenyl group can participate in subsequent curing, the vanillic acid is introduced into a molecular chain of the ink, meanwhile, migration of the vanillic acid is avoided.

The application provides a pumpkin stem medicinal material, decoction piece, standard decoction and formula granule HPLC characteristic spectrum construction method, comprising: A) extracting pumpkin stem raw materials with a solvent to obtain a test solution; B) determining the test solution by high performance liquid chromatography to obtain the HPLC characteristic spectrum of the pumpkin stem raw material; the high performance liquid chromatography chromatographic conditions are that the chromatographic column is a C18 column; the mobile phase A is acetonitrile, the mobile phase B is 0.2% phosphoric acid, and gradient elution is adopted. The application adopts high performance liquid chromatography, selects acetonitrile-0.2% phosphoric acid as the mobile phase for gradient elution, establishes the HPLC characteristic spectrum of the pumpkin stem medicinal material, decoction piece, standard decoction and formula granule with vanillic acid as a reference, and has good repeatability and precision, and the method is stable and reliable, so that the quality of the pumpkin stem medicinal material, decoction piece, standard decoction and formula granule can be controlled.

The application discloses a detection method of Yichangleng composition, and the method comprises the following steps: S1, preparation of reference solution; S2, preparation of test solution; S3, preparation of mixed control solution; S4, respectively, precisely sucking Yichangleng test solution of S2 and mixed control solution of S3; injecting into a liquid chromatograph; S5, exporting Yichangleng characteristic spectrum obtained in S4, selecting chromatographic peaks existing in chromatograms of Yichangleng solution as characteristic peaks; calculating relative peak area and relative retention time of each characteristic peak; and according to the retention time of the chromatogram of the mixed control solution, labeling the components of the peaks in the control characteristic spectrum, the method can be used for detecting gallic acid, vanillic acid, paeoniflorin, uncarine, thalictrifolin, 5-O-methylvisamminol glycoside, quercetin, kaempferol and atractylochide II, and can realize overall quality control of Yichangleng solution.

The application provides a construction method of UPLC characteristic spectrum of Qinglongyi medicinal material, standard decoction and formula granules, comprising the following steps: dissolving and extracting raw materials of the test sample by using a solvent to obtain a test solution; determining the test solution by using high performance liquid chromatography to obtain the UPLC characteristic spectrum of Qinglongyi medicinal material, standard decoction and formula granules; and the high performance liquid chromatography conditions are as follows: a C 18 column is used as the chromatographic column; acetonitrile solution is used as mobile phase A, and 0.1% formic acid aqueous solution is used as mobile phase B, and gradient elution is adopted. The application adopts the high performance liquid chromatography, selects acetonitrile-0.1% formic acid solution as the mobile phase for gradient elution, takes protocatechuic acid, vanillic acid, syringic acid, ellagic acid, rutin and quercitrin as the reference substances, establishes the UPLC characteristic spectrum method of Qinglongyi medicinal material, standard decoction and formula granules, and provides more scientific technical means for controlling the medicinal quality of Qinglongyi medicinal material, standard decoction and formula granules.

The invention belongs to the cross technical field of microbial genetic engineering and food fermentation production, and particularly relates to a vanillin degradation related gene, application and an engineering strain for producing vanillin. The gene for regulating and controlling the production of vanillin by microorganisms is a 3-ketoacyl-ACP reductase gene, and the base sequence of the gene is shown in SEQ ID NO: 1. The 3-ketoacyl-ACP reductase gene is applied to regulation and control of degradation of vanillin. And the 3-ketoacyl-ACP reductase gene is knocked out from the chassis strain to obtain the 3-ketoacyl-ACP reductase. According to the invention, the fact that the fabG gene participates in the conversion of vanillin into vanillic acid is found for the first time, then the fabG gene is further utilized to modify the strain, the modification mode is highly targeted and has a remarkable effect, and through directional knockout of fabG and further knockout of vdh genes, phdB, fabG and modABC genes, the degradation pathway of vanillin is effectively blocked, and the product loss is reduced.

The invention discloses a mcl-PHA production method based on a wood and agricultural waste composite carbon source, and relates to the technical field of bio-manufacturing, the method comprises the following steps: adopting Pseudomonas putida BWCC66157 to carry out fermentation in an improved M9 minimum culture medium; a lignin-derived carbon source (such as vanillic acid) is used as a basic substrate; respectively introducing rubber wood hydrolysate and oil palm pomace palm oil as co-substrates; the directional regulation and control of the composition of the mcl-PHA monomer are realized by regulating and controlling the ratio of the co-substrate; and extracting the PHA by adopting a conventional method and detecting the monomer composition of the PHA. The PHA yield can be increased, new monomer components can be obtained, high-valued utilization of wood carbon sources and agricultural waste is achieved, and a new technical approach is provided for production of low-cost and degradable biological materials.

The invention discloses a method for rapidly determining various phenolic acid substances in a fermentation product, and belongs to the technical field of detection. The determination method comprises the following steps: 1. Taking a to-be-detected substance, and extracting phenolic acid substances in the to-be-detected substance through an alcohol precipitation-SAX solid-phase extraction combined technology to obtain a to-be-detected sample; analyzing the to-be-detected sample by adopting ultra-high performance liquid chromatography tandem triple quadrupole mass spectrometry to obtain the content of the target substance in the to-be-detected substance; the target substance is a non-volatile phenolic acid substance. Accurate quantification of trace and trace caffeic acid, dihydrocaffeic acid, ferulic acid, chlorogenic acid, gallic acid, vanillic acid, syringic acid, p-coumaric acid, salicylic acid and (+ / -)-catechin in fermentation products within 8-15 min is achieved by combining impurity removal and concentration pretreatment with ultra-high performance liquid chromatography tandem triple quadrupole mass spectrometry. According to the method, the pretreatment mode is convenient to operate, the detection process is fast, and the detection result is accurate.

The present invention relates to a method for preparing an intermediate for the synthesis of opicapone, which comprises the nitration of vanillic acid in an acid anhydride using a nitrating agent. The present invention also relates to an intermediate for the synthesis of opicapone produced by the process. Furthermore, the present invention relates to a solution of the intermediate for the synthesis of opicapone in an acid anhydride.

The invention discloses a vanillic acid derivative containing a thiosemicarbazide structure and application of the vanillic acid derivative. The compound has relatively good oxidation resistance and alpha-glucosidase resistance activity; wherein the IC50 values of the compound 2-(4-(benzyloxy)-3-methoxybenzoyl)-N-isopropylhydrazine-1-methylsulfuryl on DPPH (1, 1-diphenyl-2-picrylhydrazyl) scavenging activity, ABTS (2, 2 '-azinobenzoyl) scavenging activity and alpha-glucosidase inhibitory activity are respectively 5.33 mu g / mL, 5.84 mu g / mL and 34.99 mu g / mL, the effect is the best, and the IC50 values are superior to those of control medicaments such as vanillic acid, vitamin C, vitamin E and acarbose. The vanillic acid derivative containing the thiosemicarbazide structure has a general formula shown in the specification,

The application relates to an unsaturated polyester resin with low styrene volatility and a preparation method thereof, and belongs to the technical field of unsaturated polyester resins. The unsaturated polyester resin with low styrene volatility is composed of the following components: unsaturated polyester resin, sodium octenyl succinate starch, composite lignin sulfonate, pine bark polyphenol, vanillic acid and styrene. The composite lignin sulfonate is composed of calcium lignosulfonate and sodium lignosulfonate in a weight ratio of 1.93-1.95:1. The preparation steps are as follows: 1) respectively weighing and drying calcium lignosulfonate and sodium lignosulfonate and sieving; 2) respectively weighing and drying pine bark polyphenol and vanillic acid; 3) adding sodium octenyl succinate starch, pine bark polyphenol, vanillic acid into the unsaturated polyester resin to mix, adding calcium lignosulfonate to mix, adding sodium lignosulfonate to mix, and then adding styrene to mix to obtain the unsaturated polyester resin with low styrene volatility. The unsaturated polyester resin with low styrene volatility has the characteristics of good inhibition effect and low cost.