42 results about "Juglans" patented technology

The present application belongs to the technical field of composite Fu tea, and particularly relates to a forsythia suspense leaf tea, walnut branch wood composite Fu tea and a preparation method thereof. The forsythia suspense leaf tea, walnut branch wood composite Fu tea is prepared by adding composite strains to forsythia suspense leaf tea, black tea and branch wood silk after steam softening, and then performing two-stage fermentation and finally microwave treatment. The mass ratio of the forsythia suspense leaf tea, black tea, branch wood silk and composite strains is 40-70:20-50:10-30:0.5-1. The composite strains are prepared by mixing Eurotium cristatum liquid, Lactobacillus plantarum liquid and Aspergillus niger liquid at a mass ratio of 5-7:2-4:1. The two-stage fermentation is primary fermentation at 32-35 DEG C for 10-14 hours, and then secondary fermentation at 24-28.5 DEG C for 19-21 days. The forsythia suspense leaf tea, walnut branch wood composite Fu tea has a golden flower density of greater than 2.0*10 5 CFU / g dry tea, high forsythiaside retention rate, weak bitterness, strong aroma and good taste.

The invention relates to a special color master batch for imitating walnut wood, a preparation method of the color master batch and a method for preparing a walnut wood imitating polyolefin floor by using the color master batch. The walnut-imitated color master batch comprises the following components in percentage by mass: 10-12% of titanium dioxide, 2.5-3.0% of carbon black, 3-5% of iron oxide red, 2-4% of iron oxide yellow, 0.5-1% of phthalocyanine blue, 1-2% of a dispersing agent and the balance of a resin carrier, and the sum of the components is 100%. According to the technical scheme, through three key technologies of accurate color matching of multiple pigments, efficient dispersion and temperature gradient control, the walnut-wood-imitating polyolefin floor achieves the level of natural walnut wood in the dimensions of color simulation degree, texture stereoscopic impression and the like, and exceeds the level of the natural walnut wood in the indexes of material cost, weather resistance, processing efficiency, environmental friendliness and the like.

The invention discloses a preparation method of a prevention and control agent for winter low-temperature freezing injury of walnuts. The prevention and control agent is prepared from the following active ingredients in percentage by mass: 0.2 part of agricultural chitosan oligosaccharide, 1 part of carboxymethyl cellulose, 7.8 parts of jasmonic acid methyl ester, 0.3 part of monopotassium phosphate, 0.1 part of compound amino acid, 0.05 part of brassinolide and 100 parts of water. The method effectively solves the problems that the annual branches of the Xinjiang walnuts in winter are prone to freezing, and the existing prevention and control means are poor in effect, reduces the conditions of branch water loss and drying, tree body weakness and even death, can obviously improve the cold resistance of the annual branches and mixed buds, greatly relieves the freezing injury symptoms after adult trees and young trees are treated, and obviously optimizes physiological indexes.

The invention discloses a traditional Chinese medicine composition for improving memory and concentration and a preparation method thereof, and relates to the technical field of traditional Chinese medicine formulas. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 12-22 parts of fructus alpiniae oxyphyllae, 15-25 parts of radix astragali, 8-16 parts of ginseng, 10-20 parts of walnuts, 10-20 parts of radix puerariae, 6-14 parts of cornel pulp, 8-18 parts of radix rehmanniae, 10-20 parts of Chinese wolfberry fruits, 15-25 parts of spina date seeds, 8-16 parts of polygala tenuifolia, 6-14 parts of rhizoma acori graminei and 4-10 parts of earthworms. According to the traditional Chinese medicine composition for improving memory and concentration and the preparation method of the traditional Chinese medicine composition, polygala ketone contained in polygala tenuifolia can specifically up-regulate hippocampal BDNF expression and doubly repair memory pathway damage caused by alcohol, volatile asarone in acorus gramineus can penetrate a blood brain barrier, and the volatile asarone is compatible with polygala tenuifolia, so that the nerve stimulation threshold value of a single component can be reduced, the intelligence promoting effect can be improved, and the traditional Chinese medicine composition can be used for improving memory and concentration. The earthworm extract can inhibit the activity of lactic dehydrogenase in the brain, and the synergistic effect mechanism of the polygala tenuifolia, the rhizoma acori graminei and the earthworm can also play a role in preventing and treating the Alzheimer's disease.

The present application relates to the field of microbial technology, and particularly relates to a bacillus megaterium YBZ13 for preventing and treating walnut leaf scorching and application thereof. The bacillus megaterium YBZ13 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.28098. The bacillus megaterium YBZ13 is a walnut rhizosphere symbiotic bacterium, and the bacterial suspension has a significant prevention and treatment effect on walnut seedling leaf scorching.

The invention belongs to the technical field of green prevention and control of plant fungal diseases, and particularly relates to a method for preparing a plant-source antibacterial liquid by using beak walnut waste and application of the botanical antibacterial liquid, the method comprises the following steps: raw material preparation: collecting branches and leaves of beak walnut, drying, crushing and sieving with a 40-mesh sieve; water extraction: soaking the crushed raw materials in water according to a material-liquid ratio (g / L) of (25-100): 1; leaching: standing and soaking for 24-48 hours under the condition of room temperature; filtration and sterilization: residues are removed through filtration, filtrate is collected, and the beak walnut branch and leaf waste antibacterial liquid is obtained.According to the antibacterial liquid, the hypha growth inhibition rate of six pathogenic fungi can reach 72% or above, and it is proved that secondary metabolites of beak walnuts have high stability in litters and are remarkable in antibacterial effect.

The present application provides a kind of wettable powder and its preparation method and application, belong to forest disease biological control technical field.The wettable powder of the present application is made of the following mass fraction components: Bacillus subtilis WB1 fermentation liquor 90-110 parts, filler 42-48 parts, auxiliary 28-32 parts.The wettable powder of Bacillus subtilis WB1 prepared by the present application has high viable count, good suspensibility, dispersibility and wettability, can be stored, transported and sprayed in large area in forest at room temperature for a long time, has broad-spectrum antibacterial property, and has good control effect on walnut bacterial black spot.The preparation method of the wettable powder of the present application is simple, low in cost, and suitable for large-scale popularization and application.

The invention discloses preparation and application of a microcapsule compounded with walnut green seedcase extract and lavender essential oil, and belongs to the technical field of botanical pesticides. The walnut green seedcase extract is prepared with an ultrasonic-assisted two-phase salting-out extraction method, lavender essential oil is compounded to serve as a core material, beta-cyclodextrin serves as a wall material, and the composite microcapsule which is good in slow release performance and has an efficient inhibition effect on botrytis cinerea is obtained with a composite coacervation method. The compound essential oil microcapsule based on the walnut green seedcase extract is a natural plant effective component, is an efficient, safe and slow-release plant source agricultural bactericide, and has a remarkable antibacterial effect on botrytis cinerea.

The present application relates to the field of microbial technology, and in particular, to a Pantoea rodasii YBZ4 for preventing and treating walnut leaf scorching and application thereof. The Pantoea rodasii YBZ4 for preventing and treating walnut leaf scorching is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.31371. The Pantoea rodasii YBZ4 can effectively reduce the occurrence of walnut seedling leaf scorching, the damage degree of walnut seedling leaves under salt stress is significantly reduced, and the prevention and treatment effect can reach more than 90%.

The application provides a lactobacillus plantarum DX7 and a method and application thereof for producing indole lactic acid, relates to the field of food microbial technology, and the lactobacillus plantarum DX7 has been preserved in the Guangdong Microbial Culture Collection Center on June 12, 2025, and the preservation number is GDMCC NO.66498. The lactobacillus plantarum DX7 in the application is significantly activated under the stimulation of deep-line walnut inner seed coat polyphenol, and can efficiently convert tryptophan into ILA. Experimental results show that the ILA yield of the strain reaches 217.56 mu g / mL. Moreover, the strain and deep-line walnut inner seed coat polyphenol can significantly relieve the colon inflammation symptoms of a DSS-induced colon inflammation mouse by gavage. The application not only provides a new strategy for efficient biological preparation of the functional component ILA, but also provides a basis and potential solution for dietary intervention of inflammatory bowel disease.

The invention discloses a lip exfoliating scrub cream, which is prepared from the following ingredients in parts by weight: 0.5 to 1.5 parts of grape (VITIS VINIFERA) seed oil, 0.5 to 1.5 parts of olea europaea (OLEA EUROPAEA) fruit oil, 0.05 to 0.15 part of tocopheryl acetate, 0.001 to 0.005 part of sodium hyaluronate, 0.1 to 0.3 part of bisabolol, 25 to 55 parts of emollient, 1 to 5 parts of wax thickening agents, 0.1 to 3 parts of preservatives, 3 to 5 parts of walnut (JUGLANS REGIA) shell powder, 40 to 60 parts of cane sugar and 0.1 to 0.5 part of essence. According to the lip exfoliating scrub cream provided by the invention, the walnut shell powder and the cane sugar are adopted as the compound abrasive, and the abrasive with different particle sizes has a synergistic effect, so that dead skin of lips can be more effectively removed, meanwhile, the excessive irritation of a single abrasive with a large particle size to the lip skin is avoided, the pain is reduced, and the use experience is improved.

The present application relates to the field of microbiological technology, and particularly relates to a walnut decay disease biocontrol bacterium Achromobacter insuavis P52 and application thereof.The Achromobacter insuavis is Achromobacter insuavis P52, which is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.28849.A strain of Achromobacter insuavis is isolated from a pot seedling of Populus trichocarpa, and the strain has a significant antagonistic effect on a walnut decay disease pathogenic fungus Cytospora chrysosperma, with an inhibition rate of 62.4%, and an in-vitro prevention and treatment effect on walnut branch decay disease of 76%.The Achromobacter insuavis in the present application has a significant prevention and treatment effect on walnut decay disease, and has a good application prospect.

The application discloses a method for improving the growth and fruiting rate of 2-3-year-old Carya illinoensis container grafting seedlings after afforestation, which comprises the following steps: selecting 2-3-year-old Carya illinoensis container grafting seedlings and young seedling container seedlings, transplanting the container seedlings and the young seedling container seedlings into planting ponds in an afforestation area, setting up a wound on the stem bark of the container seedlings and the young seedling container seedlings respectively after scratching the stem bark, binding the wounds of the container seedlings and the young seedling container seedlings by using plastic cloth, filling soil, and normally maintaining; after the wounds are fully healed, the plastic cloth is removed, the part above the wound joint of the young seedling container seedlings is cut off, and the young seedling container seedlings are normally maintained until the roots of the young seedling container seedlings and the Carya illinoensis container seedlings are alive. The method can not only protect the root system of the container seedlings by the root system protection matrix, but also can release the root system winding, enhance the root system activity, and promote the rapid growth and development of the root system after transplanting.

The application discloses a kind of lipopeptide class for preventing and treating walnut rot disease and preparation method thereof, it is related to plant disease control technical field, including the nanocarrier core of lipopeptide class loading;And the responsive material shell of the carrier core outside is covered;Wherein, the responsive material shell can be degraded under the microenvironment of walnut tree disease site, the microenvironment characteristics include pH value is lower than 6.0 and there is pectinase activity.The present application realizes the precise and efficient prevention and treatment of walnut rot disease by constructing the intelligent nanometer drug delivery system of "nuclear shell structure", realizes the targeting and on-demand release of lipopeptide in lesion site using the shell with double response to disease microenvironment, greatly improves drug utilization and prolongs the effective period;Nanocarrier core effectively protects lipopeptide activity, and its 50-200 nanometer optimized particle size and surface modification promote drug delivery and enrichment in tree body.

The utility model discloses an auxiliary tool for picking juglans mandshurica fruits, which comprises a frame provided with a cavity for accommodating fruits and an opening on the top surface, and the opening is communicated with the cavity; the fixed roller is rotationally arranged on the frame, and part of the fixed roller exceeds the opening and faces outwards; the movable roller is arranged on the frame in a sliding manner, and part of the movable roller exceeds the opening and faces outwards; the driving equipment is mounted outside the frame, and the output end of the driving equipment is coaxially and fixedly connected with the fixed roller to drive the fixed roller to rotate; wherein the frame is provided with two skewed slots, each skewed slot is provided with a first end part and a second end part, the first end part is higher than the second end part, the movable roller is arranged in a sliding manner along the skewed slot direction, and the movable roller always has a trend of moving towards the second end part under the action of gravity. According to the fruit picking device, the fixed roller rotates and is matched with the movable roller capable of sliding to clamp fruits, so that when the fixed roller and the movable roller approach the fruits, the fruits can be rolled into the cavity in a friction force mode to complete picking, and the phenomenon that the fruits are cut off by mistake during picking is avoided.

The invention provides lactobacillus plantarum DX7, a method for producing indole lactic acid by the lactobacillus plantarum DX7 and application of the lactobacillus plantarum DX7, and relates to the technical field of food microorganisms, the lactobacillus plantarum DX7 is preserved in Guangdong Microbial Culture Collection Center on June 12, 2025, and the preservation number is GDMCC NO.66498. The lactobacillus plantarum DX7 has the advantages that under the stimulation of the deep-grain walnut endopleura polyphenol, the tryptophan metabolic pathway of the lactobacillus plantarum DX7 is obviously activated, and the tryptophan can be efficiently converted into the ILA; experimental results show that the ILA yield of the strain reaches 217.56 g / mL. In addition, the bacterial strain and the deep-grained walnut endopleura polyphenol are combined for intragastric administration of DSS-induced colitis mice, and the colitis symptom can be remarkably relieved. The invention not only provides a new strategy for efficient biological preparation of the functional component ILA, but also provides a basis and a potential solution for dietary intervention of inflammatory bowel diseases.

The invention discloses a tissue culture optimization method of juglans mandshurica seedlings, and relates to the technical field of plant tissue culture, and the tissue culture optimization method comprises the following steps: S1, explant selection and disinfection: selecting current-year semi-lignified stem segments of juglans mandshurica as explants, washing the explants with clear water, placing the explants on a clean bench, soaking and disinfecting the explants with a 75% ethanol solution for 30-60 seconds, soaking and disinfecting the explants with a 0.1% HgCl2 solution for 8-10 minutes, and then washing the explants with sterile water for 5-6 times; according to the method, 6-BA, TDZ, ZT and GA3 with specific combination and concentration are added into a callus differentiation culture medium, the callus differentiation process is accurately regulated and controlled, the callus differentiation effect is improved, the hormone concentration range of the callus induction and adventitious bud proliferation stages is optimized, the formula of a rooting culture medium is adjusted, and the rooting rate of the rooting culture medium is increased. The culture medium formula and the culture conditions are optimized in stages, so that the efficient connection of all links of the tissue culture of the juglans mandshurica seedlings is realized, the seedling culture period is shortened, and the genetic stability and the growth quality of the seedlings are ensured.

This invention belongs to the field of seed germination technology for traditional Chinese medicinal materials, specifically providing a soaking solution containing walnut components, a seed dressing powder composition, and its application in the germination of *Gynostemma pentaphyllum* seeds. The soaking solution is prepared by water extraction from a mixture of dried walnut leaves, neem leaves, and moringa leaves. The seed dressing powder composition is a mixture of at least two of the following: walnut inner pericarp powder, walnut green husk powder, neem fruit powder, soapberry fruit powder, and sand. The germination method includes steps such as soaking seeds in the soaking solution, refrigeration, soaking in a bacterial suspension, dressing seeds with the seed dressing powder, germination, and sowing. This invention does not use plant hormones throughout the process, uses agricultural and forestry by-products as germination materials, is low-cost, safe and environmentally friendly, and simple to operate. The germination rate of *Gynostemma pentaphyllum* seeds reaches over 61%, providing effective technical support for the artificial cultivation of *Gynostemma pentaphyllum*.

The invention relates to the technical field of extract refining, and particularly discloses a method for preparing stevioside RM. The method comprises the following steps: (1) crushing dry stevia rebaudiana leaves, mixing with water, extracting, stirring, extracting, centrifugally filtering, performing column chromatography purification, washing with water, eluting with ethanol to obtain an eluent, and concentrating the eluent to obtain a stevioside solution; (2) adding an auxiliary stable mixed aid, cane sugar, cane sugar synthase, beta-1, 2-UDP-glucosyltransferase from dendrobenthamia grandiflora and beta-1, 3-UDP-glucosyltransferase from walnut into the stevioside solution obtained in the step (1), carrying out decoloration and desalination treatment after catalytic reaction, eluting with an ethanol-water solution, and carrying out vacuum drying to obtain the stevioside. And finally, carrying out vacuum concentration, recrystallization and drying treatment to obtain the product. According to the method, the stability of an enzyme action system in the stevioside RM preparation process can be remarkably improved.

The invention relates to juglans regia coat polysaccharide and application thereof. The preparation method comprises the following steps: extracting the mangnolia mandshurica polysaccharide PJP60 by utilizing a flash extraction technology in combination with graded alcohol precipitation, decolorizing by utilizing AB-8 macroporous resin, separating and purifying by using a DEAE-52 anion exchange chromatographic column to obtain the mangnolia mandshurica polysaccharide PJP60-I, further purifying by adopting a Sephadex G-50 gel chromatographic column, and purifying by using a macroporous resin column to obtain the mangnolia mandshurica polysaccharide PJP60-I. The molecular weight of the single component is 11250 Da, and the single component is composed of rhamnose, glucose and fructose. The cortex juglandis mandshuricae polysaccharide PJP60-Ia provided by the invention can be used for preparing an anti-inflammatory preparation, and has the advantages of high purity, good anti-inflammatory activity and the like.

The invention discloses a compound type hair dye as well as a preparation method and application thereof. Comprising an agent A and an agent B, the agent A contains indigo blue, henna quinone, a thickening agent, a surfactant, an organic solvent and water; the agent B contains a natural plant extracting solution, a thickening agent, a surfactant and water; the natural plant extract is obtained by the following steps: crushing natural plants, mixing, adding water of which the weight is 1-1.5 times of the total weight of the natural plants, carrying out ultrasonic treatment at 45-55 DEG C for 1.5-3 hours, and then centrifuging to obtain supernate, namely a natural plant extracting solution; the natural plant is selected from at least one of polygonum multiflorum, walnut green seedcase, eclipta, madder, lithospermum, ginger and sophora flower bud.

The invention provides a method for intercropping oat and triticale under a walnut forest, and belongs to the technical field of forest crop planting. Comprising the following steps: (1) selecting a walnut forest of which the land slope is 22-30 degrees and the tree age is 5-7 years; (2) cleaning under-forest sundries 2-3 months before intercropping, spreading quicklime, farmyard manure and a compound fertilizer between rows of the walnut trees as a base fertilizer, and deeply ploughing the walnut trees into soil; (3) oat and triticale are intercropped under the walnut forest, planting is not carried out with walnut trunks as the center and within the radius of 1-1.5 m of walnut trees, oat is planted at the center line position of every two adjacent rows of walnut trees to form an oat belt, and triticale is planted on the two sides of each oat belt to form triticale belts; and (4) when the oats and the triticale grow to a milk-ripe stage, cutting the oats and the triticale. According to the intercropping crop combination, the plant yield of walnut trees can be effectively increased, the intercropping crop yield can be increased through the intercropping method, the soil organic matter content can be increased, the soil fertility can be improved, and a certain soil improving effect is achieved.

The invention relates to a new saccharomycetes, in particular to a pseudomonas putida RT12 strain and an application of the pseudomonas putida RT12 strain in degradation of juglone in walnut green seedcases. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 19, 2025, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.36686. A strain of Pseudomonas putida RT12 is obtained through separation, screening and identification in Aksu Red-flag slope region in Xinjiang, and experiments on growth characteristics, physiology and biochemistry, ester production capability and tolerance of the strain verify that the strain has the activity of efficiently degrading juglone, which indicates that the Pseudomonas putida RT12 has a good application prospect, and can be used for preparing a high-efficiency degradation product of juglone. The method can be widely applied to the agricultural solid waste resource treatment industry.

A new and distinct variety of nectarine tree (Prunus persica nucipersica), which is denominated varietally as ‘Prima Diamond 7’, and which produces large fruits that mature approximately the last week of June in the San Joaquin Valley of Central California.

The invention relates to the technical field of biological medicines, and particularly discloses a food composition capable of reducing hypertension and application thereof. Comprising the following raw materials in parts by weight: 5-10 parts of ginseng, 3-6 parts of eucommia male flowers, 1-4 parts of cordyceps militaris, 5-9 parts of kudzu vine roots, 4-8 parts of dandelions, 7-12 parts of poria cocos, 7-12 parts of wheat germs, 3-7 parts of Chinese wolfberry fruits, 2-6 parts of haws, 2-5 parts of white gourds, 3-7 parts of corn stigma, 2-6 parts of cape jasmine, 2-6 parts of sea-buckthorn, 5-10 parts of cassia seeds, 4-8 parts of walnuts, 4-8 parts of peach kernels, 4-8 parts of gastrodia elata and 2-6 parts of mulberry leaves, the composition is prepared through a low-temperature extraction technology, and the extraction temperature is 35-45 DEG C; according to the invention, through scientific proportioning of a plurality of natural raw materials, such as synergistic combination of 18 components such as ginseng, eucommia male flower, cordyceps militaris and radix puerariae, the effects of tonifying qi and consolidating body resistance, reducing blood pressure, resisting oxidation, inducing diuresis and the like of each raw material are fully exerted, so that the effect of regulating blood pressure at multiple target points is achieved; the hypertension can be comprehensively conditioned from a plurality of links such as blood vessels, kidneys and nervous systems.

The invention relates to walnut (Juglans regia) green husk extract for use as a feed additive in fish feeds in the aquaculture sector, which regulates the immune system of fish and, in particular, strengthens resistance against diseases.

The invention discloses a compound ecological fertilizer and a preparation method and application thereof, and relates to the technical field of agricultural organic fertilizers, and the compound ecological fertilizer is prepared from the following raw materials in parts by mass: 24-45 parts of tussah silkworm excrement, 19-75 parts of walnut tree leaves, 12-25 parts of volcanic ash soil, 5-8 parts of humic acid and 3-6 parts of pyroligneous liquor. Ecological natural resources such as tussah silkworm excrement, walnut leaves and volcanic ash soil and agricultural wastes are used as raw materials to replace traditional fertilizers, the problem of adverse effects on soil, environment, agricultural product quality and the like caused by long-term use of the traditional agricultural fertilizers is solved, the yield and quality of crop products are improved, the soil ecosystem is restored, and the economic benefit is increased. And long-term sustainable green development of agriculture is realized.

The invention relates to the technical field of walnut planting, in particular to a pest and disease resistant walnut crossbreeding device and method.The method comprises the steps that S1, a ball body is moved to enable stamens to be placed in the ball body, and a magnetic assembly is controlled to enable openings of rubber rings to be adjusted to be attached to flower stalks; s2, enabling an arc plate to be unbalanced in stress and shake by utilizing negative pressure suction force and magnetic suction force of an air pump, and shaking off the pollen; s3, controlling a magnetic assembly to enable a rubber ring to approach a fixed ring, and moving a ball to take out the stamen; s4, adjusting the rubber ring through a magnetic assembly to control the caliber, aligning the opening of the rubber ring to the stigma of the female flower, and generating positive pressure for inflating and spraying through an air pump to finish pollination. The stamens are fixed in the ball body through opening and closing of the rubber rings, pollen on the stamens on the same branch is shaken off and collected through the arc plate, the situation that the pollen is collected on each flower one by one through a cotton swab is avoided, the ball body is inflated through the air pump, the pollen is sprayed on female flower stigmas, the manual pollen taking and pollination time is shortened, and the efficiency is improved.

The invention discloses a spectroscopy-based walnut leaf chlorine element content rapid inversion method, apparatus and device, and a medium. The method comprises the following steps: collecting walnut leaves; spectral information of the walnut leaves is extracted; screening a sensitive wave band and a vegetation index which are highly related to the chlorine element content based on the spectral information of the walnut leaves; and inputting the screened sensitive wave band and the vegetation index into a trained walnut leaf chlorine element content prediction model to realize prediction of the walnut leaf chlorine element content. Therefore, the chlorine content of the walnut leaves can be rapidly and accurately quantified, and a reliable method is provided for monitoring and preventing the leaf scorch disease.

The invention belongs to the field of microorganisms, and relates to bacillus amyloliquefaciens JDF-3 as well as a fungicide and application thereof, the bacillus amyloliquefaciens JDF-3 is preserved in China General Microbiological Culture Collection Center on May 26, 2025, the preservation number is CGMCC No.34670, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain JDF-3 has an inhibition effect on pathogenic bacteria of walnut bacterial black spot, can effectively inhibit various plant disease pathogenic bacteria, shows broad-spectrum antibacterial activity, is environment-friendly, and can be widely applied to prevention and treatment of plant diseases.