24 results about "Juglans" patented technology

The present application belongs to the technical field of composite Fu tea, and particularly relates to a forsythia suspense leaf tea, walnut branch wood composite Fu tea and a preparation method thereof. The forsythia suspense leaf tea, walnut branch wood composite Fu tea is prepared by adding composite strains to forsythia suspense leaf tea, black tea and branch wood silk after steam softening, and then performing two-stage fermentation and finally microwave treatment. The mass ratio of the forsythia suspense leaf tea, black tea, branch wood silk and composite strains is 40-70:20-50:10-30:0.5-1. The composite strains are prepared by mixing Eurotium cristatum liquid, Lactobacillus plantarum liquid and Aspergillus niger liquid at a mass ratio of 5-7:2-4:1. The two-stage fermentation is primary fermentation at 32-35 DEG C for 10-14 hours, and then secondary fermentation at 24-28.5 DEG C for 19-21 days. The forsythia suspense leaf tea, walnut branch wood composite Fu tea has a golden flower density of greater than 2.0*10 5 CFU / g dry tea, high forsythiaside retention rate, weak bitterness, strong aroma and good taste.

The present application relates to the field of microbial technology, and particularly relates to a bacillus megaterium YBZ13 for preventing and treating walnut leaf scorching and application thereof. The bacillus megaterium YBZ13 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.28098. The bacillus megaterium YBZ13 is a walnut rhizosphere symbiotic bacterium, and the bacterial suspension has a significant prevention and treatment effect on walnut seedling leaf scorching.

The invention belongs to the technical field of green prevention and control of plant fungal diseases, and particularly relates to a method for preparing a plant-source antibacterial liquid by using beak walnut waste and application of the botanical antibacterial liquid, the method comprises the following steps: raw material preparation: collecting branches and leaves of beak walnut, drying, crushing and sieving with a 40-mesh sieve; water extraction: soaking the crushed raw materials in water according to a material-liquid ratio (g / L) of (25-100): 1; leaching: standing and soaking for 24-48 hours under the condition of room temperature; filtration and sterilization: residues are removed through filtration, filtrate is collected, and the beak walnut branch and leaf waste antibacterial liquid is obtained.According to the antibacterial liquid, the hypha growth inhibition rate of six pathogenic fungi can reach 72% or above, and it is proved that secondary metabolites of beak walnuts have high stability in litters and are remarkable in antibacterial effect.

The present application provides a kind of wettable powder and its preparation method and application, belong to forest disease biological control technical field.The wettable powder of the present application is made of the following mass fraction components: Bacillus subtilis WB1 fermentation liquor 90-110 parts, filler 42-48 parts, auxiliary 28-32 parts.The wettable powder of Bacillus subtilis WB1 prepared by the present application has high viable count, good suspensibility, dispersibility and wettability, can be stored, transported and sprayed in large area in forest at room temperature for a long time, has broad-spectrum antibacterial property, and has good control effect on walnut bacterial black spot.The preparation method of the wettable powder of the present application is simple, low in cost, and suitable for large-scale popularization and application.

The present application relates to the field of microbial technology, and in particular, to a Pantoea rodasii YBZ4 for preventing and treating walnut leaf scorching and application thereof. The Pantoea rodasii YBZ4 for preventing and treating walnut leaf scorching is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.31371. The Pantoea rodasii YBZ4 can effectively reduce the occurrence of walnut seedling leaf scorching, the damage degree of walnut seedling leaves under salt stress is significantly reduced, and the prevention and treatment effect can reach more than 90%.

The application provides a lactobacillus plantarum DX7 and a method and application thereof for producing indole lactic acid, relates to the field of food microbial technology, and the lactobacillus plantarum DX7 has been preserved in the Guangdong Microbial Culture Collection Center on June 12, 2025, and the preservation number is GDMCC NO.66498. The lactobacillus plantarum DX7 in the application is significantly activated under the stimulation of deep-line walnut inner seed coat polyphenol, and can efficiently convert tryptophan into ILA. Experimental results show that the ILA yield of the strain reaches 217.56 mu g / mL. Moreover, the strain and deep-line walnut inner seed coat polyphenol can significantly relieve the colon inflammation symptoms of a DSS-induced colon inflammation mouse by gavage. The application not only provides a new strategy for efficient biological preparation of the functional component ILA, but also provides a basis and potential solution for dietary intervention of inflammatory bowel disease.

The present application relates to the field of microbiological technology, and particularly relates to a walnut decay disease biocontrol bacterium Achromobacter insuavis P52 and application thereof.The Achromobacter insuavis is Achromobacter insuavis P52, which is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.28849.A strain of Achromobacter insuavis is isolated from a pot seedling of Populus trichocarpa, and the strain has a significant antagonistic effect on a walnut decay disease pathogenic fungus Cytospora chrysosperma, with an inhibition rate of 62.4%, and an in-vitro prevention and treatment effect on walnut branch decay disease of 76%.The Achromobacter insuavis in the present application has a significant prevention and treatment effect on walnut decay disease, and has a good application prospect.

The application discloses a method for improving the growth and fruiting rate of 2-3-year-old Carya illinoensis container grafting seedlings after afforestation, which comprises the following steps: selecting 2-3-year-old Carya illinoensis container grafting seedlings and young seedling container seedlings, transplanting the container seedlings and the young seedling container seedlings into planting ponds in an afforestation area, setting up a wound on the stem bark of the container seedlings and the young seedling container seedlings respectively after scratching the stem bark, binding the wounds of the container seedlings and the young seedling container seedlings by using plastic cloth, filling soil, and normally maintaining; after the wounds are fully healed, the plastic cloth is removed, the part above the wound joint of the young seedling container seedlings is cut off, and the young seedling container seedlings are normally maintained until the roots of the young seedling container seedlings and the Carya illinoensis container seedlings are alive. The method can not only protect the root system of the container seedlings by the root system protection matrix, but also can release the root system winding, enhance the root system activity, and promote the rapid growth and development of the root system after transplanting.

The application discloses a kind of lipopeptide class for preventing and treating walnut rot disease and preparation method thereof, it is related to plant disease control technical field, including the nanocarrier core of lipopeptide class loading;And the responsive material shell of the carrier core outside is covered;Wherein, the responsive material shell can be degraded under the microenvironment of walnut tree disease site, the microenvironment characteristics include pH value is lower than 6.0 and there is pectinase activity.The present application realizes the precise and efficient prevention and treatment of walnut rot disease by constructing the intelligent nanometer drug delivery system of "nuclear shell structure", realizes the targeting and on-demand release of lipopeptide in lesion site using the shell with double response to disease microenvironment, greatly improves drug utilization and prolongs the effective period;Nanocarrier core effectively protects lipopeptide activity, and its 50-200 nanometer optimized particle size and surface modification promote drug delivery and enrichment in tree body.

The invention discloses a tissue culture optimization method of juglans mandshurica seedlings, and relates to the technical field of plant tissue culture, and the tissue culture optimization method comprises the following steps: S1, explant selection and disinfection: selecting current-year semi-lignified stem segments of juglans mandshurica as explants, washing the explants with clear water, placing the explants on a clean bench, soaking and disinfecting the explants with a 75% ethanol solution for 30-60 seconds, soaking and disinfecting the explants with a 0.1% HgCl2 solution for 8-10 minutes, and then washing the explants with sterile water for 5-6 times; according to the method, 6-BA, TDZ, ZT and GA3 with specific combination and concentration are added into a callus differentiation culture medium, the callus differentiation process is accurately regulated and controlled, the callus differentiation effect is improved, the hormone concentration range of the callus induction and adventitious bud proliferation stages is optimized, the formula of a rooting culture medium is adjusted, and the rooting rate of the rooting culture medium is increased. The culture medium formula and the culture conditions are optimized in stages, so that the efficient connection of all links of the tissue culture of the juglans mandshurica seedlings is realized, the seedling culture period is shortened, and the genetic stability and the growth quality of the seedlings are ensured.

This invention belongs to the field of seed germination technology for traditional Chinese medicinal materials, specifically providing a soaking solution containing walnut components, a seed dressing powder composition, and its application in the germination of *Gynostemma pentaphyllum* seeds. The soaking solution is prepared by water extraction from a mixture of dried walnut leaves, neem leaves, and moringa leaves. The seed dressing powder composition is a mixture of at least two of the following: walnut inner pericarp powder, walnut green husk powder, neem fruit powder, soapberry fruit powder, and sand. The germination method includes steps such as soaking seeds in the soaking solution, refrigeration, soaking in a bacterial suspension, dressing seeds with the seed dressing powder, germination, and sowing. This invention does not use plant hormones throughout the process, uses agricultural and forestry by-products as germination materials, is low-cost, safe and environmentally friendly, and simple to operate. The germination rate of *Gynostemma pentaphyllum* seeds reaches over 61%, providing effective technical support for the artificial cultivation of *Gynostemma pentaphyllum*.

The invention discloses a compound type hair dye as well as a preparation method and application thereof. Comprising an agent A and an agent B, the agent A contains indigo blue, henna quinone, a thickening agent, a surfactant, an organic solvent and water; the agent B contains a natural plant extracting solution, a thickening agent, a surfactant and water; the natural plant extract is obtained by the following steps: crushing natural plants, mixing, adding water of which the weight is 1-1.5 times of the total weight of the natural plants, carrying out ultrasonic treatment at 45-55 DEG C for 1.5-3 hours, and then centrifuging to obtain supernate, namely a natural plant extracting solution; the natural plant is selected from at least one of polygonum multiflorum, walnut green seedcase, eclipta, madder, lithospermum, ginger and sophora flower bud.

The invention relates to a new saccharomycetes, in particular to a pseudomonas putida RT12 strain and an application of the pseudomonas putida RT12 strain in degradation of juglone in walnut green seedcases. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 19, 2025, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.36686. A strain of Pseudomonas putida RT12 is obtained through separation, screening and identification in Aksu Red-flag slope region in Xinjiang, and experiments on growth characteristics, physiology and biochemistry, ester production capability and tolerance of the strain verify that the strain has the activity of efficiently degrading juglone, which indicates that the Pseudomonas putida RT12 has a good application prospect, and can be used for preparing a high-efficiency degradation product of juglone. The method can be widely applied to the agricultural solid waste resource treatment industry.

A new and distinct variety of nectarine tree (Prunus persica nucipersica), which is denominated varietally as ‘Prima Diamond 7’, and which produces large fruits that mature approximately the last week of June in the San Joaquin Valley of Central California.

The invention relates to walnut (Juglans regia) green husk extract for use as a feed additive in fish feeds in the aquaculture sector, which regulates the immune system of fish and, in particular, strengthens resistance against diseases.

The invention discloses a compound ecological fertilizer and a preparation method and application thereof, and relates to the technical field of agricultural organic fertilizers, and the compound ecological fertilizer is prepared from the following raw materials in parts by mass: 24-45 parts of tussah silkworm excrement, 19-75 parts of walnut tree leaves, 12-25 parts of volcanic ash soil, 5-8 parts of humic acid and 3-6 parts of pyroligneous liquor. Ecological natural resources such as tussah silkworm excrement, walnut leaves and volcanic ash soil and agricultural wastes are used as raw materials to replace traditional fertilizers, the problem of adverse effects on soil, environment, agricultural product quality and the like caused by long-term use of the traditional agricultural fertilizers is solved, the yield and quality of crop products are improved, the soil ecosystem is restored, and the economic benefit is increased. And long-term sustainable green development of agriculture is realized.

The invention discloses a spectroscopy-based walnut leaf chlorine element content rapid inversion method, apparatus and device, and a medium. The method comprises the following steps: collecting walnut leaves; spectral information of the walnut leaves is extracted; screening a sensitive wave band and a vegetation index which are highly related to the chlorine element content based on the spectral information of the walnut leaves; and inputting the screened sensitive wave band and the vegetation index into a trained walnut leaf chlorine element content prediction model to realize prediction of the walnut leaf chlorine element content. Therefore, the chlorine content of the walnut leaves can be rapidly and accurately quantified, and a reliable method is provided for monitoring and preventing the leaf scorch disease.

The application belongs to the field of molecular breeding, and specifically relates to m 6 A RNA methylation modification gene in creating new drought-resistant walnut germplasm, through the function research of a RNA methyltransferase gene and a demethylase gene, the core regulation role of m Juglans regia A in the drought resistance of walnut is revealed. 6 A methyltransferase gene JrMTA And demethylase gene JrALKBH9B The function research reveals that m 6 A modification plays a core regulation role in the drought resistance of walnut. JrALKBH9B The knockout by using CRISPR / Cas9 technology can significantly improve the m 6 A level of walnut, enhance the ability to remove reactive oxygen species (ROS) and reduce water loss, thereby greatly improving the drought resistance. JrMTA On the contrary, the loss of m 6 A leads to the decrease of the m A level, so that the plant is sensitive to drought. The application provides a key target for the molecular precise improvement of drought-resistant walnut varieties.

The invention relates to application of a mortierella alpina agent, which is prepared by the following method: inoculating mortierella alpina into a culture medium for fermentation culture, centrifuging, and taking supernate; the name of the bacterial strain of the mortierella alpina is Mortierella alpina RS-01, and the preservation number of the mortierella alpina is CGMCC (China General Microbiological Culture Collection Center) No.41944. The invention further discloses a preparation method of the mortierella alpina. The mortierella alpina agent provided by the invention can be used for preventing and treating pathogenic bacteria of ginseng rust rot, pathogenic bacteria of muskmelon fusarium wilt, pathogenic bacteria of melon and fruit rot, pathogenic bacteria of apple fruit rot, pathogenic bacteria of northern corn leaf blight, pathogenic bacteria of seedling blight, pathogenic bacteria of walnut canker, pathogenic bacteria of poplar canker and pathogenic bacteria of pepper anthracnose; the compound has the best antibacterial effect on pathogenic bacteria of blueberry stem canker, pathogenic bacteria of apple shell monochromatic alternaria, pathogenic bacteria of tobacco brown spot, and especially pathogenic bacteria of ginseng rust rot.

The present application relates to a Mortierella alpina and application. The strain name is Mortierella alpina RS-01, the preservation number is CGMCC No.41944, the preservation time is May 21, 2025, and the preservation place is China General Microbiological Culture Collection Center (CGMCC), No. 3, Yihuang Road, Beichen, Beijing, China. The Mortierella alpina provided by the present application can be used for the pathogenic bacteria of ginseng rust rot, melon wilt, melon fruit rot, apple fruit rot, corn large spot, seedling stand, walnut canker, poplar canker, pepper anthracnose, blueberry stem canker, apple shell monochrome septum, tobacco brown spot, especially the best bacteriostatic effect on the pathogenic bacteria of ginseng rust rot.

The invention belongs to the technical field of molecular markers, particularly relates to an early screening method for low-browning coracoid walnut germplasm resources based on transcription factor expression quantity, and particularly provides a molecular marker related to coracoid walnut germplasm browning, and the molecular marker is a transcription factor AsbHLH68 and / or AsMYB232 gene or an encoding protein thereof, the nucleotide sequence of the AsbHLH68 gene is shown as SEQ ID NO: 5 or a sequence which has more than 90% of homology and has the same function with the nucleotide sequence of the AsbHLH68 gene; the nucleotide sequence of the AsMYB232 gene is as shown in SEQ ID NO: 6; or a sequence which has more than 90% of homology and has the same function. By the adoption of the technical scheme, the breeding cost is reduced, the breeding efficiency is improved, the seedlings can be checked on a large scale in the nursery stage, and most of high-browning-risk inferior single plants can be removed before transplanting and afforestation.

The present application relates to a Paenibacillus polymyxa strain Y31, a microbial inoculum prepared therefrom and applications thereof. The strain Y31 can effectively inhibit the growth of Dothiorella gregaria, and field control tests show that the comprehensive prevention and control effect of the microbial inoculum prepared from the strain Y31 on the disease is as high as 72% or more in the early stage of the disease. Antagonistic experiments show that the strain Y31 also has good bacteriostatic effect on Dothiorella septospora and Dothiorella needle red rot fungus, and is suitable for being used as a broad-spectrum antibacterial agent in the biocontrol practice of pine fungal diseases. Meanwhile, the strain Y31 also has obvious inhibitory effect on bacterial diseases such as willow bacterial wilt fungus, pear fire blight fungus, walnut bacterial black spot fungus and kiwi fruit canker fungus, and can be used for the prevention and control of diseases such as willow wilt. In addition, the strain Y31 also has functions such as nitrogen fixation, phosphorus and potassium solubilization, and has certain salt and alkali tolerance, and can be used for promoting growth and disease resistance of plants in saline-alkali land.

The invention provides a cosmetic composition with functions of moisturizing skin, whitening skin and improving wrinkles. The cosmetic composition of the present invention contains a frog and melon extract and a walnut extract, and can maintain skin moisture for a long period of time even after application is stopped, and provides an excellent moisturizing effect and an excellent whitening effect by reducing the melanin content of skin cells. The skin wrinkles are improved by increasing the biosynthesis rate of collagen.

The invention discloses a walnut male flower bud physiological differentiation period accurate prediction method based on multi-dimensional analysis, and relates to the technical field of plant physiology, horticulture and data analysis. The objective of the invention is to solve the problem in the prior art that a male flower bud physiological differentiation starting point is difficult to accurately determine due to insufficient observation means time resolution, lack of structural quantification capability and weak multi-dimensional variable coupling modeling. According to the method, through high-frequency synchronous sampling and multi-scale anatomical analysis, three structural changes of meristem cell division activity enhancement, vascular bundle primary formation and floral primordium initial protrusion are definitely defined as a judgment basis for physiological differentiation starting; multi-source data such as phenology, environment temperature and humidity, endogenous nutrition states, exogenous regulation and control effects and tree body growth indexes are synchronously fused, and a multi-factor coupling prediction model based on an XGBoost algorithm is constructed. Quantitative and high-precision prediction of the starting time of the physiological differentiation of the walnut male flower buds can be realized.