53 results about "Juglans" patented technology

The invention discloses Delftia tsuhuensis and an application of the Delftia tsuhuensis in prevention and treatment of plant diseases. The Delftia tsuhuensis CDJ2 is preserved in the Guangdong Microbial Culture Collection Center on October 18, 2024, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 65290. The strain has a good prevention and treatment effect on citrus canker, and the prevention and treatment effect can reach 84%. Meanwhile, the strain can also effectively prevent and treat soft rot of taro, bacterial basal rot of rice, bacterial wilt of crops, kiwifruit canker, angular leaf spot of mango, black rot of brassicaceous vegetables, tomato scab, gummosis of sugarcane, bacterial wilt of walnuts and bacterial leaf spot of plums. Wide application prospects are realized in the aspects of preventing and treating diseases of crops and commercial crops and promoting healthy growth of plants.

The invention relates to mortierella alpina and application thereof. The strain name is Mortierella alpina RS-01, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.41944, the preservation time is May 21, 2025, and the preservation location is CGMCC (China General Microbiological Culture Collection Center), No. 3, No.1 yard, West Beichen Road, Chaoyang District, Beijing, China. The mortierella alpina provided by the invention can be used for pathogenic bacteria of ginseng rust rot, pathogenic bacteria of muskmelon fusarium wilt, pathogenic bacteria of pythium aphanidermatum, pathogenic bacteria of apple fruit rot, pathogenic bacteria of northern corn leaf blight, pathogenic bacteria of seedling blight, pathogenic bacteria of walnut canker, pathogenic bacteria of poplar canker, pathogenic bacteria of pepper anthracnose and pathogenic bacteria of blueberry stem canker; the compound has the best antibacterial effect on pathogenic bacteria of apple shell monochromatic alternaria, pathogenic bacteria of tobacco brown spot, and especially pathogenic bacteria of ginseng rust rot.

The invention discloses a hair dye and a preparation method thereof. The hair dye comprises an agent A and an agent B, the agent A comprises the following substances in percentage by mass: 1-2% of a gallnut extract, 2-4% of an oak gall extract, 1-2% of a juglans regia extract, 1-2% of a codonopsis pilosula extract, 1-2% of an astragalus membranaceus extract, 1-2% of an angelica sinensis extract, 2-4% of an iris tectorum root extract, 1-3% of methionine, 1-2% of a penetrant, 1-2% of an antioxidant, 1-2% of a bacteriostatic agent and 1-2% of a reducing agent; 15-20% of an emulsifier, 15-20% of a thickening agent, and the balance of water; the agent B is prepared from the following substances in percentage by mass: 2 to 4 percent of mordant, 1 to 2 percent of keratin, 1 to 2 percent of methionine, 2 to 3 percent of penetrant, 1 to 2 percent of bacteriostatic agent, 2 to 3 percent of antioxidant, 20 to 25 percent of emulsifier, 15 to 25 percent of thickening agent and the balance of water. The black coloring agent disclosed by the invention is selected from natural plant extracts such as the oak gall extract, the gallnut extract and the juglans regia extract, so that the black coloring agent is small in irritation, and the influence of the middle-aged and elderly people on the skin and the hair quality of the middle-aged and elderly people in the hair dyeing process can be reduced.

The present application belongs to the technical field of composite Fu tea, and particularly relates to a forsythia suspense leaf tea, walnut branch wood composite Fu tea and a preparation method thereof. The forsythia suspense leaf tea, walnut branch wood composite Fu tea is prepared by adding composite strains to forsythia suspense leaf tea, black tea and branch wood silk after steam softening, and then performing two-stage fermentation and finally microwave treatment. The mass ratio of the forsythia suspense leaf tea, black tea, branch wood silk and composite strains is 40-70:20-50:10-30:0.5-1. The composite strains are prepared by mixing Eurotium cristatum liquid, Lactobacillus plantarum liquid and Aspergillus niger liquid at a mass ratio of 5-7:2-4:1. The two-stage fermentation is primary fermentation at 32-35 DEG C for 10-14 hours, and then secondary fermentation at 24-28.5 DEG C for 19-21 days. The forsythia suspense leaf tea, walnut branch wood composite Fu tea has a golden flower density of greater than 2.0*10 5 CFU / g dry tea, high forsythiaside retention rate, weak bitterness, strong aroma and good taste.

The invention relates to a special color master batch for imitating walnut wood, a preparation method of the color master batch and a method for preparing a walnut wood imitating polyolefin floor by using the color master batch. The walnut-imitated color master batch comprises the following components in percentage by mass: 10-12% of titanium dioxide, 2.5-3.0% of carbon black, 3-5% of iron oxide red, 2-4% of iron oxide yellow, 0.5-1% of phthalocyanine blue, 1-2% of a dispersing agent and the balance of a resin carrier, and the sum of the components is 100%. According to the technical scheme, through three key technologies of accurate color matching of multiple pigments, efficient dispersion and temperature gradient control, the walnut-wood-imitating polyolefin floor achieves the level of natural walnut wood in the dimensions of color simulation degree, texture stereoscopic impression and the like, and exceeds the level of the natural walnut wood in the indexes of material cost, weather resistance, processing efficiency, environmental friendliness and the like.

The invention discloses a preparation method of a prevention and control agent for winter low-temperature freezing injury of walnuts. The prevention and control agent is prepared from the following active ingredients in percentage by mass: 0.2 part of agricultural chitosan oligosaccharide, 1 part of carboxymethyl cellulose, 7.8 parts of jasmonic acid methyl ester, 0.3 part of monopotassium phosphate, 0.1 part of compound amino acid, 0.05 part of brassinolide and 100 parts of water. The method effectively solves the problems that the annual branches of the Xinjiang walnuts in winter are prone to freezing, and the existing prevention and control means are poor in effect, reduces the conditions of branch water loss and drying, tree body weakness and even death, can obviously improve the cold resistance of the annual branches and mixed buds, greatly relieves the freezing injury symptoms after adult trees and young trees are treated, and obviously optimizes physiological indexes.

The invention discloses a traditional Chinese medicine composition for improving memory and concentration and a preparation method thereof, and relates to the technical field of traditional Chinese medicine formulas. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 12-22 parts of fructus alpiniae oxyphyllae, 15-25 parts of radix astragali, 8-16 parts of ginseng, 10-20 parts of walnuts, 10-20 parts of radix puerariae, 6-14 parts of cornel pulp, 8-18 parts of radix rehmanniae, 10-20 parts of Chinese wolfberry fruits, 15-25 parts of spina date seeds, 8-16 parts of polygala tenuifolia, 6-14 parts of rhizoma acori graminei and 4-10 parts of earthworms. According to the traditional Chinese medicine composition for improving memory and concentration and the preparation method of the traditional Chinese medicine composition, polygala ketone contained in polygala tenuifolia can specifically up-regulate hippocampal BDNF expression and doubly repair memory pathway damage caused by alcohol, volatile asarone in acorus gramineus can penetrate a blood brain barrier, and the volatile asarone is compatible with polygala tenuifolia, so that the nerve stimulation threshold value of a single component can be reduced, the intelligence promoting effect can be improved, and the traditional Chinese medicine composition can be used for improving memory and concentration. The earthworm extract can inhibit the activity of lactic dehydrogenase in the brain, and the synergistic effect mechanism of the polygala tenuifolia, the rhizoma acori graminei and the earthworm can also play a role in preventing and treating the Alzheimer's disease.

The present application relates to the field of microbial technology, and particularly relates to a bacillus megaterium YBZ13 for preventing and treating walnut leaf scorching and application thereof. The bacillus megaterium YBZ13 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.28098. The bacillus megaterium YBZ13 is a walnut rhizosphere symbiotic bacterium, and the bacterial suspension has a significant prevention and treatment effect on walnut seedling leaf scorching.

The invention discloses an application of walnut YX4 as a receptor material in agrobacterium tumefaciens-mediated genetic transformation. The invention relates to the field of genetic transformation, and particularly discloses a method for carrying out genetic transformation by taking Juglans regia YX4 CGMCC (China General Microbiological Culture Collection Center) No.46084 as an acceptor material. According to the invention, genotype walnuts with high somatic embryo induction rate, strong regeneration capacity and high genetic transformation efficiency are screened as genetic transformation receptors, and an efficient genetic transformation system and method are optimized and established. By adopting the transformation method disclosed by the invention, the transformation efficiency (P = 0.008) of agrobacterium infection is remarkably improved. The screened excellent receptor material of the YX-4 genotype is named as YX4, and is preserved in the China General Microbiological Culture Collection Center (CGMCC). The screening of excellent receptor materials and the optimization of a genetic transformation system have important practical significance for establishing a walnut rapid propagation system, and the commercialization process of walnut breeding is promoted.

The invention belongs to the technical field of green prevention and control of plant fungal diseases, and particularly relates to a method for preparing a plant-source antibacterial liquid by using beak walnut waste and application of the botanical antibacterial liquid, the method comprises the following steps: raw material preparation: collecting branches and leaves of beak walnut, drying, crushing and sieving with a 40-mesh sieve; water extraction: soaking the crushed raw materials in water according to a material-liquid ratio (g / L) of (25-100): 1; leaching: standing and soaking for 24-48 hours under the condition of room temperature; filtration and sterilization: residues are removed through filtration, filtrate is collected, and the beak walnut branch and leaf waste antibacterial liquid is obtained.According to the antibacterial liquid, the hypha growth inhibition rate of six pathogenic fungi can reach 72% or above, and it is proved that secondary metabolites of beak walnuts have high stability in litters and are remarkable in antibacterial effect.

The present application provides a kind of wettable powder and its preparation method and application, belong to forest disease biological control technical field.The wettable powder of the present application is made of the following mass fraction components: Bacillus subtilis WB1 fermentation liquor 90-110 parts, filler 42-48 parts, auxiliary 28-32 parts.The wettable powder of Bacillus subtilis WB1 prepared by the present application has high viable count, good suspensibility, dispersibility and wettability, can be stored, transported and sprayed in large area in forest at room temperature for a long time, has broad-spectrum antibacterial property, and has good control effect on walnut bacterial black spot.The preparation method of the wettable powder of the present application is simple, low in cost, and suitable for large-scale popularization and application.

The invention relates to the technical field of fruit tree cultivation, and discloses a breeding device for planting carya illinoensis, which comprises a nursery pond, a water storage pipe is fixedly mounted in the nursery pond, a plurality of culture pipes are fixedly mounted on the surface of the water storage pipe, and each culture pipe is vertically arranged on the upper surface of the water storage pipe; a water supply pipe is fixedly installed at one end of the nursery pond and communicated with the water storage pipe, and a drainage pipe is fixedly installed at the other end of the nursery pond and communicated with an inner cavity of the nursery pond. According to the breeding device, the culture tube is arranged, the walnut seeds are placed at the upper end of the culture tube, the lower sides of the walnut seeds make contact with the culture solution in the tube, it is guaranteed that the walnut seeds can absorb sufficient water, nutrition and oxygen, and due to the fact that the nutrient solution in the culture tube is large in amount, the liquid level declines slowly, and frequent liquid supplementing is not needed; the problem that the seedling raising temperature is affected by frequent water replenishing is solved, and the seedling raising efficiency is improved.

The present invention provides a method for planting multiple varieties of forage grass under a walnut forest, belonging to the field of agricultural planting technology. The method comprises the following steps: (1) soaking forage grass seeds in warm water and a selenium solution in sequence and then drying to obtain pretreated forage grass seeds; (2) mixing the pretreated forage grass seeds obtained in step (1) with a coating agent to obtain coated forage grass seeds; (3) mixing the coated forage grass seeds obtained in step (2) with crushed walnut leaves and then sowing them under the walnut forest. The present invention can significantly improve the germination performance and growth performance of the forage grass seeds by pretreating and coating the forage grass seeds in warm water and a selenium solution. The present invention also mixes the forage grass seeds with crushed walnut leaves before sowing the forage grass seeds. The crushed walnut leaves can provide suitable environmental conditions and nutrients for the germination of the forage grass seeds, which is conducive to further improving the yield of forage grass.

The invention discloses preparation and application of a microcapsule compounded with walnut green seedcase extract and lavender essential oil, and belongs to the technical field of botanical pesticides. The walnut green seedcase extract is prepared with an ultrasonic-assisted two-phase salting-out extraction method, lavender essential oil is compounded to serve as a core material, beta-cyclodextrin serves as a wall material, and the composite microcapsule which is good in slow release performance and has an efficient inhibition effect on botrytis cinerea is obtained with a composite coacervation method. The compound essential oil microcapsule based on the walnut green seedcase extract is a natural plant effective component, is an efficient, safe and slow-release plant source agricultural bactericide, and has a remarkable antibacterial effect on botrytis cinerea.

The present application relates to the field of microbial technology, and in particular, to a Pantoea rodasii YBZ4 for preventing and treating walnut leaf scorching and application thereof. The Pantoea rodasii YBZ4 for preventing and treating walnut leaf scorching is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.31371. The Pantoea rodasii YBZ4 can effectively reduce the occurrence of walnut seedling leaf scorching, the damage degree of walnut seedling leaves under salt stress is significantly reduced, and the prevention and treatment effect can reach more than 90%.

The invention discloses a walnut bough top grafting method. The top grafting method comprises the following steps that in May to July every year, walnut twigs needing to be grafted are selected as scions, square bud bark is taken down, and the square bud bark is covered with wet cloth for standby application; selecting a smooth branch from the rootstock, then cutting off bark with the same size as a bud bark from the branch to obtain a cut, and reserving a water outlet at the lowest position; putting the square bud bark into a stock, and then binding the square bud bark with the stock by using an adhesive tape; after 5-7 days, the adhesive tape is unfastened, the petiole roots falling off from the bud bark are removed, and the adhesive tape is bound again. By means of the grafting method, the grafting survival rate is 80% or above, and the plant growth vigor is good.

The application provides a lactobacillus plantarum DX7 and a method and application thereof for producing indole lactic acid, relates to the field of food microbial technology, and the lactobacillus plantarum DX7 has been preserved in the Guangdong Microbial Culture Collection Center on June 12, 2025, and the preservation number is GDMCC NO.66498. The lactobacillus plantarum DX7 in the application is significantly activated under the stimulation of deep-line walnut inner seed coat polyphenol, and can efficiently convert tryptophan into ILA. Experimental results show that the ILA yield of the strain reaches 217.56 mu g / mL. Moreover, the strain and deep-line walnut inner seed coat polyphenol can significantly relieve the colon inflammation symptoms of a DSS-induced colon inflammation mouse by gavage. The application not only provides a new strategy for efficient biological preparation of the functional component ILA, but also provides a basis and potential solution for dietary intervention of inflammatory bowel disease.

The invention discloses a lip exfoliating scrub cream, which is prepared from the following ingredients in parts by weight: 0.5 to 1.5 parts of grape (VITIS VINIFERA) seed oil, 0.5 to 1.5 parts of olea europaea (OLEA EUROPAEA) fruit oil, 0.05 to 0.15 part of tocopheryl acetate, 0.001 to 0.005 part of sodium hyaluronate, 0.1 to 0.3 part of bisabolol, 25 to 55 parts of emollient, 1 to 5 parts of wax thickening agents, 0.1 to 3 parts of preservatives, 3 to 5 parts of walnut (JUGLANS REGIA) shell powder, 40 to 60 parts of cane sugar and 0.1 to 0.5 part of essence. According to the lip exfoliating scrub cream provided by the invention, the walnut shell powder and the cane sugar are adopted as the compound abrasive, and the abrasive with different particle sizes has a synergistic effect, so that dead skin of lips can be more effectively removed, meanwhile, the excessive irritation of a single abrasive with a large particle size to the lip skin is avoided, the pain is reduced, and the use experience is improved.

The present application relates to the field of microbiological technology, and particularly relates to a walnut decay disease biocontrol bacterium Achromobacter insuavis P52 and application thereof.The Achromobacter insuavis is Achromobacter insuavis P52, which is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.28849.A strain of Achromobacter insuavis is isolated from a pot seedling of Populus trichocarpa, and the strain has a significant antagonistic effect on a walnut decay disease pathogenic fungus Cytospora chrysosperma, with an inhibition rate of 62.4%, and an in-vitro prevention and treatment effect on walnut branch decay disease of 76%.The Achromobacter insuavis in the present application has a significant prevention and treatment effect on walnut decay disease, and has a good application prospect.

The application discloses a method for improving the growth and fruiting rate of 2-3-year-old Carya illinoensis container grafting seedlings after afforestation, which comprises the following steps: selecting 2-3-year-old Carya illinoensis container grafting seedlings and young seedling container seedlings, transplanting the container seedlings and the young seedling container seedlings into planting ponds in an afforestation area, setting up a wound on the stem bark of the container seedlings and the young seedling container seedlings respectively after scratching the stem bark, binding the wounds of the container seedlings and the young seedling container seedlings by using plastic cloth, filling soil, and normally maintaining; after the wounds are fully healed, the plastic cloth is removed, the part above the wound joint of the young seedling container seedlings is cut off, and the young seedling container seedlings are normally maintained until the roots of the young seedling container seedlings and the Carya illinoensis container seedlings are alive. The method can not only protect the root system of the container seedlings by the root system protection matrix, but also can release the root system winding, enhance the root system activity, and promote the rapid growth and development of the root system after transplanting.

The application discloses a kind of lipopeptide class for preventing and treating walnut rot disease and preparation method thereof, it is related to plant disease control technical field, including the nanocarrier core of lipopeptide class loading;And the responsive material shell of the carrier core outside is covered;Wherein, the responsive material shell can be degraded under the microenvironment of walnut tree disease site, the microenvironment characteristics include pH value is lower than 6.0 and there is pectinase activity.The present application realizes the precise and efficient prevention and treatment of walnut rot disease by constructing the intelligent nanometer drug delivery system of "nuclear shell structure", realizes the targeting and on-demand release of lipopeptide in lesion site using the shell with double response to disease microenvironment, greatly improves drug utilization and prolongs the effective period;Nanocarrier core effectively protects lipopeptide activity, and its 50-200 nanometer optimized particle size and surface modification promote drug delivery and enrichment in tree body.

The utility model discloses an auxiliary tool for picking juglans mandshurica fruits, which comprises a frame provided with a cavity for accommodating fruits and an opening on the top surface, and the opening is communicated with the cavity; the fixed roller is rotationally arranged on the frame, and part of the fixed roller exceeds the opening and faces outwards; the movable roller is arranged on the frame in a sliding manner, and part of the movable roller exceeds the opening and faces outwards; the driving equipment is mounted outside the frame, and the output end of the driving equipment is coaxially and fixedly connected with the fixed roller to drive the fixed roller to rotate; wherein the frame is provided with two skewed slots, each skewed slot is provided with a first end part and a second end part, the first end part is higher than the second end part, the movable roller is arranged in a sliding manner along the skewed slot direction, and the movable roller always has a trend of moving towards the second end part under the action of gravity. According to the fruit picking device, the fixed roller rotates and is matched with the movable roller capable of sliding to clamp fruits, so that when the fixed roller and the movable roller approach the fruits, the fruits can be rolled into the cavity in a friction force mode to complete picking, and the phenomenon that the fruits are cut off by mistake during picking is avoided.

The invention provides lactobacillus plantarum DX7, a method for producing indole lactic acid by the lactobacillus plantarum DX7 and application of the lactobacillus plantarum DX7, and relates to the technical field of food microorganisms, the lactobacillus plantarum DX7 is preserved in Guangdong Microbial Culture Collection Center on June 12, 2025, and the preservation number is GDMCC NO.66498. The lactobacillus plantarum DX7 has the advantages that under the stimulation of the deep-grain walnut endopleura polyphenol, the tryptophan metabolic pathway of the lactobacillus plantarum DX7 is obviously activated, and the tryptophan can be efficiently converted into the ILA; experimental results show that the ILA yield of the strain reaches 217.56 g / mL. In addition, the bacterial strain and the deep-grained walnut endopleura polyphenol are combined for intragastric administration of DSS-induced colitis mice, and the colitis symptom can be remarkably relieved. The invention not only provides a new strategy for efficient biological preparation of the functional component ILA, but also provides a basis and a potential solution for dietary intervention of inflammatory bowel diseases.

The invention discloses a tissue culture optimization method of juglans mandshurica seedlings, and relates to the technical field of plant tissue culture, and the tissue culture optimization method comprises the following steps: S1, explant selection and disinfection: selecting current-year semi-lignified stem segments of juglans mandshurica as explants, washing the explants with clear water, placing the explants on a clean bench, soaking and disinfecting the explants with a 75% ethanol solution for 30-60 seconds, soaking and disinfecting the explants with a 0.1% HgCl2 solution for 8-10 minutes, and then washing the explants with sterile water for 5-6 times; according to the method, 6-BA, TDZ, ZT and GA3 with specific combination and concentration are added into a callus differentiation culture medium, the callus differentiation process is accurately regulated and controlled, the callus differentiation effect is improved, the hormone concentration range of the callus induction and adventitious bud proliferation stages is optimized, the formula of a rooting culture medium is adjusted, and the rooting rate of the rooting culture medium is increased. The culture medium formula and the culture conditions are optimized in stages, so that the efficient connection of all links of the tissue culture of the juglans mandshurica seedlings is realized, the seedling culture period is shortened, and the genetic stability and the growth quality of the seedlings are ensured.

The invention discloses a method for regulating and controlling the polymerization degree of tannin in diaphragma juglandis based on a composite enzymolysis technology, and relates to the technical field of plant active ingredient processing. Comprising the following steps: (1) raw material pretreatment: freeze-drying, crushing and sieving dried diaphragma juglandis, and adding a pretreatment solution for pretreatment; (2) step enzymolysis: sequentially carrying out cellulase treatment, tannase and beta-glucosidase compounding treatment and peroxidase treatment; and (3) inactivation and purification: carrying out heat treatment to terminate enzyme activity, and then carrying out gradient elution and purification by adopting macroporous resin. The invention provides a method for regulating and controlling the polymerization degree of walnut diaphragma juglandis tannin on the basis of a composite enzymolysis technology, which can reduce the polymerization degree under a mild condition and maintain relatively high biological activity at the same time.

This invention belongs to the field of seed germination technology for traditional Chinese medicinal materials, specifically providing a soaking solution containing walnut components, a seed dressing powder composition, and its application in the germination of *Gynostemma pentaphyllum* seeds. The soaking solution is prepared by water extraction from a mixture of dried walnut leaves, neem leaves, and moringa leaves. The seed dressing powder composition is a mixture of at least two of the following: walnut inner pericarp powder, walnut green husk powder, neem fruit powder, soapberry fruit powder, and sand. The germination method includes steps such as soaking seeds in the soaking solution, refrigeration, soaking in a bacterial suspension, dressing seeds with the seed dressing powder, germination, and sowing. This invention does not use plant hormones throughout the process, uses agricultural and forestry by-products as germination materials, is low-cost, safe and environmentally friendly, and simple to operate. The germination rate of *Gynostemma pentaphyllum* seeds reaches over 61%, providing effective technical support for the artificial cultivation of *Gynostemma pentaphyllum*.

The invention relates to the technical field of extract refining, and particularly discloses a method for preparing stevioside RM. The method comprises the following steps: (1) crushing dry stevia rebaudiana leaves, mixing with water, extracting, stirring, extracting, centrifugally filtering, performing column chromatography purification, washing with water, eluting with ethanol to obtain an eluent, and concentrating the eluent to obtain a stevioside solution; (2) adding an auxiliary stable mixed aid, cane sugar, cane sugar synthase, beta-1, 2-UDP-glucosyltransferase from dendrobenthamia grandiflora and beta-1, 3-UDP-glucosyltransferase from walnut into the stevioside solution obtained in the step (1), carrying out decoloration and desalination treatment after catalytic reaction, eluting with an ethanol-water solution, and carrying out vacuum drying to obtain the stevioside. And finally, carrying out vacuum concentration, recrystallization and drying treatment to obtain the product. According to the method, the stability of an enzyme action system in the stevioside RM preparation process can be remarkably improved.

The invention relates to juglans regia coat polysaccharide and application thereof. The preparation method comprises the following steps: extracting the mangnolia mandshurica polysaccharide PJP60 by utilizing a flash extraction technology in combination with graded alcohol precipitation, decolorizing by utilizing AB-8 macroporous resin, separating and purifying by using a DEAE-52 anion exchange chromatographic column to obtain the mangnolia mandshurica polysaccharide PJP60-I, further purifying by adopting a Sephadex G-50 gel chromatographic column, and purifying by using a macroporous resin column to obtain the mangnolia mandshurica polysaccharide PJP60-I. The molecular weight of the single component is 11250 Da, and the single component is composed of rhamnose, glucose and fructose. The cortex juglandis mandshuricae polysaccharide PJP60-Ia provided by the invention can be used for preparing an anti-inflammatory preparation, and has the advantages of high purity, good anti-inflammatory activity and the like.

The invention discloses a compound type hair dye as well as a preparation method and application thereof. Comprising an agent A and an agent B, the agent A contains indigo blue, henna quinone, a thickening agent, a surfactant, an organic solvent and water; the agent B contains a natural plant extracting solution, a thickening agent, a surfactant and water; the natural plant extract is obtained by the following steps: crushing natural plants, mixing, adding water of which the weight is 1-1.5 times of the total weight of the natural plants, carrying out ultrasonic treatment at 45-55 DEG C for 1.5-3 hours, and then centrifuging to obtain supernate, namely a natural plant extracting solution; the natural plant is selected from at least one of polygonum multiflorum, walnut green seedcase, eclipta, madder, lithospermum, ginger and sophora flower bud.

The walnuts are rich in protein. Wild pecans in Changbai Mountains in northeast China are rich in yield, and residues after oil pressing are mostly used as feed. Active substances such as subtilin, polymyxin, nystatin and brevibacterium peptide are generated in the growth process of bacillus subtilis thalli, enzymes such as alpha-amylase, protease, lipase and cellulase are synthesized by the bacillus subtilis thalli, and the northeast walnut oil extraction residues rich in protein can be degraded. Therefore, the invention provides the method for preparing the bacillus subtilis degradation liquid from the northeast walnut oil extraction residues. The method is beneficial to comprehensive utilization of the northeast walnut oil extraction residues.