57 results about "Colletotrichum" patented technology

The invention belongs to the field of pesticides, and relates to a 2-cyano-3-disubstituted phenyl acrylate compound as well as a preparation method and application thereof in pesticides. Carrying out alcoholysis reaction on the benzonitrile compound a and a hydrogen chloride absolute ethyl alcohol solution to obtain an intermediate b; carrying out condensation reaction on the intermediate b and ethyl cyanoacetate under the action of alkali to obtain an intermediate c; the intermediate c and a substituted compound are subjected to a substitution reaction under the action of alkali and a catalyst, and the 2-cyano-3-disubstituted phenyl acrylate compound I is obtained. The 2-cyano-3-disubstituted phenylacrylate compound provided by the invention has good bactericidal activity, and has relatively high bactericidal activity on pathogenic bacteria of chlorosclerotium, fusarium, acrocyst, colletotrichum, cercospora, verticillium and black beancurd skin; the compound can be used for preparing bactericides in the fields of agriculture, horticulture, industry and the like, and has the advantages of broad spectrum, high efficiency, low toxicity and environmental friendliness.

The invention relates to bacillus velezensis B313-6 and application thereof in inhibiting colletotrichum gloeosporioides of ginseng, and belongs to the technical field of agricultural microorganisms. The invention discloses bacillus velezensis B313-6, the preservation number of the bacillus velezensis B313-6 is CGMCC (China General Microbiological Culture Collection Center) No.31782, and the invention provides a preparation method of the bacillus velezensis B313-6 microbial inoculum. The bacillus velezensis B313-6 and the microbial inoculum thereof have high bacteriostatic activity on Colletotrichum panaccolica of ginseng, effectively reduce the occurrence of anthracnose of ginseng or American ginseng, are safe to the growth of ginseng or American ginseng, have no phytotoxicity, have no pollution to the environment, and can be widely applied to the field of biological preparations of ginseng or American ginseng, so that the bacillus velezensis B313-6 and the microbial inoculum thereof can be widely applied to the field of biological preparations of ginseng or American ginseng. The compound has good biopesticide development potential and application prospect in the aspect of preventing and treating anthracnose of ginseng or American ginseng, and has a certain growth promoting effect on ginseng roots.

The invention discloses a penicillium fungus GS218, a penicillium fungus GS218 conidium suspension and a preparation method and application of the penicillium fungus GS218 conidium suspension. The penicillium fungus GS218 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC M 20241477, and the penicillium fungus GS218 is separated from fruits of trifoliate orange in a Hunan Institute of Gardening Institute of Hunan Province in the East Lake Street, Hibiscus District, Changsha City, Hunan Province. The penicillium fungus has a relatively good antagonistic effect on HLB13 fusarium buchneri, colletotrichum spinosus, fusarium solani, fusarium oxysporum cucumber specialized type and botrytis cinerea; the composition can be used for effectively preventing and treating carrot diseases caused by HLB13 fusarium brucellosis, pepper anthracnose caused by colletotrichum spinosus, pepper root rot caused by fusarium solani, cucumber fusarium wilt caused by fusarium oxysporum cucumber specialization and botrytis cinerea caused by botrytis cinerea, and has remarkable and stable technical effects; the method has wide application value in the technical field of microbial strain application.

The invention discloses low-temperature-resistant rice endophytic streptomyces with biological prevention and control effects on common crop diseases and application of the low-temperature-resistant rice endophytic streptomyces, relates to the field of microorganisms, and aims to solve the problems that an existing rice blast biocontrol strain cannot play a stable effect under a low-temperature condition and is poor in rice blast control effect. The low-temperature-resistant rice endophytic streptomycete is alfalfa streptomycete F05 and is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is February 17, 2025, and the preservation number is CGMCC No.33539. The streptomyces medicaginis F05 disclosed by the invention can stably play a biological prevention and control role at a low temperature of 5-15 DEG C. The compound has broad-spectrum antibacterial performance, is used for inhibiting corn stalk rot bacteria, green bean colletotrichum, rice bakania, botrytis cinerea and rhizoctonia solani, and can also be used for decomposing starch and cellulose.

The invention provides streptomyces LZZY-S3 and application thereof, and belongs to the field of microorganisms. The streptomyces LZZY-S3 provided by the invention enriches strains for biological control, and realizes the dual functions of plant pathogenic bacterium resistance and plant growth promotion. The streptomyces LZZY-S3 has a broad-spectrum fungus inhibition function and can be applied to plant pathogenic bacterium resistance; pathogenic bacteria include: European tea leaf disease pathogen, Botryosphaeria spp., Gibberella zeae pathogen, fusarium oxysporum pathogen, wheat root rot pathogen, rice seedling rot pathogen, pumpkin gummy stem blight pathogen, wheat stem rot pathogen, Trichothecium roseum, grape white rot pathogen, and Colletotrichum graminum pathogen. The pathogenic bacteria include: European tea leaf disease pathogen, Botryosphaeria spp. Wherein the inhibition rate of tea cake disease germs reaches 91.2%. The streptomyces LZZY-S3 can also be applied to plant growth promotion, preparation of nitrogen-fixing bacterial agents, preparation and production of ACC deaminase bacterial agents and the like.

The invention relates to the field of agricultural biotechnology and plant protection, and particularly provides application of 2-heptanone to preparation of a preparation for preventing and treating plant diseases. The bactericidal composition has a strong bacteriostatic effect on litchi colletotrichum gloeosporioides, strawberry colletotrichum gloeosporioides, mango leaf blight, pawpaw colletotrichum gloeosporioides, wheat gibberellic disease, mango colletotrichum gloeosporioides, tomato fusarium wilt, banana fusarium wilt, banana colletotrichum gloeosporioides and corn root rot. The fumigant developed on the basis of 2-heptanone and used for preventing and treating phytopathogen is expected to be used for preventing and treating compound diseases caused by various pathogenic bacteria, or comprehensively preventing and controlling different crop diseases in a crop rotation system, and has important development value.

The invention discloses a single-chain alkylamino glycerol compound, a preparation method and application. The structural general formula of the single-chain alkylamino glycerol compound is R-NH-CH2-CHOH-CH2OH, the single-chain alkylamino glycerol compound is used for preventing and controlling peach monilinia and / or botryosphaeria dothidea, and R is a C8-C18 straight chain or branched chain and saturated or unsaturated alkyl. The single-chain alkylamino glycerol compound provided by the invention can be prepared by reacting glycidol and alkylamine under mild conditions, has a remarkable inhibition effect on peach monilinia and botryosphaeria dothidea, and can induce fungal membrane rupture, anti-oxidation system imbalance and lipid peroxidation, so that fungus killing is realized. The device can be widely used for fruit and vegetable postharvest preservation, orchard spraying and green pesticide development.

The invention discloses a shared fusarium and application thereof, and belongs to the technical field of plant endophytic fungi. The preservation number of the shared fusarium obtained by separation is CGMCC (China General Microbiological Culture Collection Center) No.41090. It is found through experiments that the strain G3-29 has an excellent inhibition effect on various pathogenic bacteria such as fusarium graminearum, fusarium pseudograminearum, colletotrichum gloeosporioides, sclerotinia sclerotiorum, wheat root rot bihilum and maize bihilum, and has a good application prospect. The strain G3-29 has an obvious promotion effect on wheat growth, and has an obvious biological control effect on wheat scab. In addition, when the strain G3-29 is used for treating elymus dahuricus and corn, the occurrence of elymus dahuricus leaf spot disease and corn leaf spot disease can also be obviously reduced. A new strain resource is provided for prevention and control of gramineous plant diseases, and a theoretical basis is provided for effective prevention and control of various gramineous plant diseases.

A new variety of garden bean (Phaseolus vulgaris L.) designated ‘Sanford’ is provided. Garden bean variety ‘Sanford’ is characterized by its dark green pods in mainly the 4 sieve class, heat tolerance, strong, erect and vigorous plant habit, and resistance to Colletotrichum lindemuthiunum (Anthracnose) and resistance to many races of Uromyces appendiculatus (Rust).

The application provides a Lecanicillium attenuatum strain and application thereof, the classification and name of the strain are LIO314, which has been preserved in the China Center for Type Culture Collection on June 18, 2025. Lecanicillium aphanocladii The address for preservation is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20251419. Research shows that the strain exhibits significant inhibitory effect on plant pathogenic fungi such as Fusarium graminearum and Colletotrichum gloeosporoides, can efficiently kill pepper aphids, has nitrogen fixation, potassium and starch decomposition activities, can significantly promote the growth of rice, wheat and lettuce seedlings, can be used as an efficient, environment-friendly and low-cost biological control agent, simultaneously has plant growth regulation function, can be used as a biological bacteria organic fertilizer, and has wide development and utilization value and application prospect in agricultural production.

The invention belongs to the technical field of microbial prevention and control of plant diseases and insect pests, and particularly discloses a plant disease biocontrol bacillus cereus strain LCBC246 which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the strain is CGMCC No.33323. The invention further discloses a preparation method of the plant disease biocontrol bacillus cereus strain LCBC246. The LCBC246 strain provided by the invention is a gram-positive bacterium, has a very strong bacteriostatic effect on colletotrichum fucicola, the bacteriostatic rate is 92.1%, the disease control rate on the colletotrichum fucicola is 90.8%, and the LCBC246 strain has a very good inhibitory effect on other important plateau characteristic crop pathogenic bacteria and also has a remarkable crop growth promoting property. The bacterial inhibition rates of fermentation liquor and volatile substances of the strain LCBC246 on colletotrichum gloeosporioides of macadamia nuts are 62.70% and 76.58% respectively; the bacillus amyloliquefaciens can generate sterilization metabolites such as cellulase, amylase and siderophore, and has a very strong broad-spectrum bacteriostatic effect on various plant pathogenic fungi. Therefore, the strain LCBC246 and the metabolite thereof have the application value of developing bacterial biopesticides.

The invention relates to a tetrahydro-beta-carboline bacteriostatic agent as well as a synthesis method and application thereof. The method comprises the following steps: by taking a nicotinic acid analogue as a raw material, adding a solvent anhydrous dichloromethane for dissolving, reacting with thionyl chloride under the condition of oil bath heating to prepare a corresponding acyl chloride substrate, then carrying out amidation reaction with a tetrahydro-beta-carboline compound, adding a quenching agent for quenching reaction, and carrying out vacuum concentration to remove dichloromethane to obtain the heterocyclic compound. The compound has the application of inhibiting phytopathogen. The phytopathogens are Dioscorea nipponica, Phytophthora parasitica var nicotianae, rhizoctonia solani, Colletotrichum gloeosporioides, Colletotrichum frutescens and Coriolus versicolor. The synthesis process is simple, and the product purity is high.

The invention belongs to the technical field of biological medicine, discloses a bicyclic terpenoid ester compound, a preparation method and application, and particularly relates to a bicyclic terpenoid ester compound separation and purification method and alpha-glucosidase inhibitory activity of the bicyclic terpenoid ester compound, and the bicyclic terpenoid ester compound separation and purification method comprises the steps of colletotrichum fasciculatum large-scale fermentation, methanol ultrasonic extraction, vacuum concentration and ethyl acetate extraction. According to the method, the bicyclic terpenoid ester compounds in the colletotrichum fasciatum are separated and purified through the steps of normal phase column chromatography, high performance liquid chromatography separation and purification and the like, and various modern spectrum analysis technologies such as nuclear magnetic resonance spectrum, high resolution mass spectrum, infrared spectrum, ultraviolet-visible spectrum and circular dichroism spectrum are combined, so that the content of the bicyclic terpenoid ester compounds in the colletotrichum fasciatum is improved. The bicyclic terpenoid ester compound is obtained from a colletotrichum fasciculatum solid fermentation product, and the alpha-glucosidase inhibitory activity of the compound is determined. The compound is obtained by being separated from a solid rice fermentation product of a colletotrichum dematium strain, and the colletotrichum dematium strain is separated from the root part of dianthus dematium. The compound has relatively strong inhibitory activity on alpha-glucosidase, has an IC50 value of 16.8 [mu] M, and can be used for preparing hypoglycemic drugs.

The invention belongs to the technical field of plant disease prevention and treatment, and particularly relates to a polypeptide PP alpha for preventing and treating fungal diseases and application thereof. The invention discloses application of polypeptide PP alpha in inhibiting plant pathogenic fungi and preventing and treating plant diseases caused by the plant pathogenic fungi, the polypeptide PP alpha is a yeast alpha mating factor, and the amino acid sequence of the polypeptide PP alpha is shown as SEQ ID No.1. Experiments prove that the polypeptide PP alpha can significantly inhibit spore germination and appressorium formation of various plant pathogenic fungi such as pyricularia oryzae, cucurbitaceae colletotrichum spinosus, colletotrichum cryptum and botrytis cinerea, so that the infection process of the fungi is blocked. The polypeptide PP alpha provided by the invention has broad-spectrum antifungal activity, can be used as a novel green bactericide for preventing and treating fungal diseases of crops, and has a wide application prospect.

The invention relates to the technical field of molecular detection, and particularly discloses a molecular target for detecting colletotrichum ginseng. Aiming at the technical problems of long time consumption, poor specificity, difficulty in realizing accurate quantification of pathogenic bacteria in soil and the like in the existing colletotrichum ginseng detection method, the invention provides a section of specific long nucleotide sequence of colletotrichum ginseng as a molecular target, and the nucleotide sequence is shown as SEQ ID NO: 1. A specific primer group designed based on the molecular target, a kit containing the primer group and a non-diagnostic purpose detection method jointly form a complete detection system, and rapid, sensitive and specific qualitative and accurate quantitative detection of the colletotrichum ginseng in ginseng plant tissues, seeds and soil samples can be realized; and a reliable technical tool is provided for early warning of ginseng anthracnose, dynamic monitoring of pathogenic bacteria and scientific prevention and control.

The invention discloses application of 2-methylbutyric acid in prevention and treatment of postharvest diseases of mangifera indica. The diseases comprise mangifera indica anthracnose and mangifera indica stem-end rot. Wherein the pathogenic bacteria of the mango anthracnose comprise Asian colletotrichum gloeosporioides; the pathogenic bacteria of the mango stem-end rot comprise botryosphaeria dothidea and / or physosphaeria sojae. It is found for the first time that 2-methylbutyric acid has significant inhibitory activity on main pathogens after mango picking, and especially shows an efficient fumigation bacteriostatic effect on colletotrichum asiaticum causing mango anthracnose, botryosphaeria dothidea causing mango stem-end rot, and soybean mesotheca dothidea causing mango stem-end rot. 2-methylbutyric acid is mild in smell, so that the adverse effect on the original flavor of mangifera indica can be reduced to a greater extent.

The present invention relates to the field of pesticides, and relates to a 2-cyano-3-disubstituted phenyl acrylate compound, a preparation method therefor, and a use thereof in pesticides. Benzonitrile compound a and a hydrogen chloride anhydrous ethanol solution undergo an alcoholysis reaction to obtain intermediate b; the intermediate b and ethyl cyanoacetate undergo a condensation reaction under the action of an alkali to obtain intermediate c; and the intermediate c and a substitution compound undergo a substitution reaction under the action of an alkali and a catalyst to obtain 2-cyano-3-disubstituted phenyl acrylate compound I. The provided 2-cyano-3-disubstituted phenyl acrylate compound has good bactericidal activity, and has high bactericidal activity against pathogenic bacteria of Ustilaginoidea, Fusarium, Gaeumannomyces, Colletotrichum, Cercospora, Verticillium, Valsa, and Puccinia. The compound can be used for preparing bactericides in the fields of agriculture, horticulture, and industry, and has the advantages of broad spectrum, high efficiency, low toxicity and environmental friendliness.

The invention discloses an N,N'-bis-monoterpenoid imidazole quaternary ammonium salt and a synthesis method and application thereof, and belongs to the field of antibacterial materials. The synthesis method of the N,N'-bis-monoterpenoid imidazole quaternary ammonium salt comprises the following steps: adding N-monoterpenoid imidazole and a halogenated product containing a monoterpenoid group to an organic solvent for quaternization reaction, and then performing purification treatment to obtain the N,N'-bis-monoterpenoid imidazole quaternary ammonium salt; the N-monoterpenoid imidazole is N-hydrogenated imidazole or N-tetrahydrogeranyl imidazole; and the halogenated product containing a monoterpenoid group is any one of hydrogenated imidazole bromide, hydrogenated imidazole chloride, tetrahydrogeranyl bromide, tetrahydrogeranyl chloride and isobornyl methyl bromide. The N,N'-bis-monoterpenoid imidazole quaternary ammonium salt synthesized by the present invention can be used to inhibit plant pathogens such as rice sheath blight, pine blight, tea fruit spiny colleorhiza, watermelon wilt, tobacco black shank pathogen, loquat anthracnose pathogen, Coriolus versicolor, and Fusarium verticillium.

This invention discloses the application of the natural product santalol as an agricultural fungicide in plant disease control, belonging to the field of plant disease prevention and control. It shows good inhibitory effects against bacterial diseases such as rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, cabbage black rot, tomato bacterial spot, kiwifruit canker, tobacco bacterial wilt, cucumber bacterial angular leaf spot, and potato bacterial wilt. It also shows good inhibitory effects against viral diseases such as tobacco mosaic virus; and against fungal diseases such as rice rhizome blight, wheat scab, pepper wilt, *Botrytis cinerea*, or *Colletotrichum sorghum*. It can be used in the preparation of plant fungicides. Santalol is a natural plant extract and is environmentally friendly. This invention provides a new use for the natural product santalol, enabling its application in the agricultural field for plant disease control and providing a new avenue for the research and development of new pesticides.

The invention belongs to the technical field of plant disease prevention and control, and specifically relates to a use of vitex essential oil in preventing and controlling plant diseases. The plant diseases prevented and controlled by vitex essential oil are root rot or anthracnose. Antibacterial tests have shown that vitex essential oil has a certain inhibitory effect on onion anthracnose pathogens, ash anthracnose pathogens, Fusarium solani, Fusarium oxysporum, and Fusarium odoriferum, among which the inhibitory effect on onion anthracnose pathogens is the most obvious.

The invention provides a lecanicillium lecanii strain and application thereof, the classification name of the lecanicillium lecanii strain is Lecanicillium aphanocladiii LI0314, and the lecanicillium lecanii strain is preserved in the China Center for Type Culture Collection on June 18, 2025. The preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20251419. Researches show that the strain has a remarkable inhibition effect on plant pathogens such as fusarium graminearum and colletotrichum laevis, can efficiently kill pepper aphids, has nitrogen fixation, potassium decomposition and starch decomposition activities, can remarkably promote growth of seedlings of rice, wheat, lettuce and the like, and has a good application prospect. The strain can be used as a high-efficiency, environment-friendly and low-cost biological control microbial agent, has a plant growth regulation function, can be used as a biological bacterial organic fertilizer, and has wide development and utilization values and application prospects in agricultural production.

The application discloses a strain of Streptomyces clyindrosporus 11-7 and application thereof, and belongs to the technical field of microorganisms. The strain of Streptomyces clyindrosporus 11-7 disclosed by the application has a preservation number of CCTCC NO: M 2025222. The strain of Streptomyces clyindrosporus 11-7 disclosed by the application has the best inhibitory activity on Colletotrichum chlorophyti, Colletotrichum plurivorum and Rigidoporus lignosus, and the inhibitory rates are 96.75%, 95.89% and 92.94% respectively. The volatile substances produced by the strain of Streptomyces clyindrosporus 11-7 have inhibitory effects on various pathogenic bacteria. The strain of Streptomyces clyindrosporus 11-7 can fix nitrogen, produce ammonia and produce indole acetic acid.

The invention discloses streptomyces fulvus 11-7 and application thereof, and belongs to the technical field of microorganisms. The invention discloses a streptomyces fulvus strain 11-7, and the preservation number of the streptomyces fulvus strain 11-7 is CCTCC (China Center for Type Culture Collection) NO: M 2025222. The streptomyces fulvus 11-7 disclosed by the invention has the optimal bacteriostatic activity on Colletotrichum chlorophyti, Colletotrichum plurivorin and Rigidoporus ligosus, and the bacteriostatic rates of the streptomyces fulvus 11-7 are respectively 96.75%, 95.89% and 92.94%. The streptomyces fulvus 11-7 disclosed by the invention has the advantages that the streptomyces fulvus 11-7 can be used for preparing the antibacterial agent for preventing and treating the pathogenic bacteria of the Colletotrichum chlorophyti, the Colletotrichum plurivorin and the Rigidoporus ligosus; volatile substances generated by the streptomyces fulvus 11-7 have an inhibition effect on a plurality of pathogenic bacteria. The streptomyces fulvus 11-7 can be used for fixing nitrogen, producing ammonia and producing indoleacetic acid.

The invention discloses an endophytic fungus AP12 capable of promoting growth of andrographis paniculata plants and accumulation of active ingredient content and application of the endophytic fungus AP12. The colletotrichum sp. AP12 endophytic fungus provided by the invention can promote the growth of a common andrographis herb plant and the accumulation of the content of effective components, and after the colletotrichum sp. AP12 is applied and cultured under a pot culture condition, the leaf area of the common andrographis herb can be obviously increased, the fresh weight and the dry weight can be increased, the accumulation of the content of dry matters can be increased, and the growth of the common andrographis herb plant can be obviously promoted; meanwhile, the accumulation of active ingredients of andrographolide, neoandrographolide, 14-deoxidized andrographolide and dehydroandrographolide of andrographis paniculata can be remarkably promoted, the content of total lactone is increased, and it is shown that the endophytic fungi AP12 are beneficial endophytic fungi capable of promoting growth and metabolism of andrographis paniculata; and reference is provided for application and development of the endophytic fungi in promotion of growth of andrographis paniculata and accumulation of effective component content.

The invention discloses an in-vitro high-throughput inoculation method of tea tree colletotrichum gloeosporioides, and belongs to the technical field of tea tree biology. The method comprises the following steps: preparing a spore suspension with the concentration gradient (absorbance) OD600 of 0.4, 0.6, 0.8 and 1.0 from the Colletotrichum calamiae, inoculating the spore suspension to an in-vitro tea tree leaf in an injection manner, then placing the leaf in a high-throughput microenvironment culture device, and culturing for 4 days under the conditions of 28 DEG C, 75% relative humidity and 12h illumination / 12h darkness, thereby accurately evaluating the infection efficiency of the pathogenic bacteria. According to the method, a standardized inoculation process is combined with high-throughput microenvironment control, so that the problems of long period, non-uniform environment and low efficiency of a traditional method are solved, and the method has the outstanding advantages of short inoculation period, good experimental result consistency and capability of realizing batch high-throughput screening; the method can be directly used for tea tree colletotrichum gloeosporioides resistance identification, pathogen toxicity analysis and bactericide screening.

The invention belongs to the technical field of biological agents, and particularly relates to trichoderma harzianum, a biocontrol agent, a bio-organic fertilizer, a method for preventing and treating plant diseases and application. The invention provides a trichoderma harzianum P11 with the preservation number of CCTCC NO: M 2024648. The trichoderma harzianum P11 can improve the relative expression quantity of disease-resistant positive correlation genes PnCAD, PnSN1 and PnDEFL1 in plants and improve the activity of disease-resistant related enzyme activities SOD, POD and CAT, and the trichoderma harzianum P11 can damage cell membranes of pathogenic bacteria and inhibit the growth of the disease pathogenic bacteria. The trichoderma harzianum P11 has good rhizosphere colonization capability. The result of the embodiment shows that the trichoderma harzianum P11 can reduce the morbidity of panax notoginseng field root rot, and can also antagonize ginseng alternaria alternata, magnaporthe oryzae, polygonatum kingianum colletotrichum, geotrichum candidum, alternaria solani, cylindrosporium destroyed, phytophthora capsici and conidiospora spiraea.

The invention discloses a method for biosynthesizing nano platinum by using space mutant bacteria PS04-17 and application of the method. The invention provides a novel application of a space mutant strain PS04-17 strain in biosynthesis of nano platinum particles. Researches show that the PS04-17 strain has a reduction capability on hexachloroplatinic acid; the method for biologically synthesizing nano platinum by reducing hexachloroplatinic acid in a liquid fermentation culture medium by using the PS04-17 strain is simple and convenient, mild in condition, low in cost and environment-friendly. The invention provides more new strain resources with high reduction efficiency on hexachloroplatinic acid, and fills the blank in the field of synthesis of nano platinum by microorganisms. Meanwhile, the PS04-17 synthesized nano platinum can prevent and control various plant pathogens, the bacteriostasis rate of the PS04-17 synthesized nano platinum on colletotrichum litchii and peronophythora litchii can reach 100%, the PS04-17 synthesized nano platinum can also be used for preventing and controlling wheat stem rot, the prevention and control efficiency reaches 78.2%, and a new solution is provided for prevention and control of crop diseases.

The invention belongs to the technical field of biology, and particularly relates to a microbial marker combination for evaluating the quality of polygonatum sibiricum and application, the microbial marker combination comprises one or more of Aspergillus, Cercophora, Escherichia. Shigilla, Mycoplasm, Muribacaceae, Pseudomonas, Colletotrichum and Asaia, a method and an evaluation model for evaluating the quality of polygonatum sibiricum are established by utilizing the microbial marker combination, the sensitivity is 100.0%, the specificity is 72.7%, and the detection result is accurate. AUC is 89.8%, the method has high accuracy, specificity and sensitivity, and the evaluation method is economical, practical and easy and convenient to operate and can be widely applied to quality evaluation of rhizoma polygonati.

The invention belongs to the technical field of biology, and particularly relates to a microbial marker combination for evaluating the quality of polygonatum sibiricum and application, the microbial marker combination comprises one or more of Aspergillus, Cercophora, Escherichia. Shigilla, Mycoplasm, Muribacaceae, Pseudomonas, Colletotrichum and Asaia, a method and an evaluation model for evaluating the quality of polygonatum sibiricum are established by utilizing the microbial marker combination, the sensitivity is 100.0%, the specificity is 72.7%, the AUC is 89.8%, and the evaluation model is used for evaluating the quality of polygonatum sibiricum. The method has high accuracy, specificity and sensitivity, and the evaluation method is economical, practical and easy and convenient to operate and can be widely applied to quality evaluation of rhizoma polygonati.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus velezensis and application thereof. The bacillus velezensis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC 25698. The strain has a strong specific antagonistic effect on streptomyces scabies X-1 (Streptomyces scabies X-1), the prevention effect of a pot experiment reaches 70.90%, and the strain has no pathogenic risk on potato tubers. A further research shows that the bacillus velezensis XM18-5 has a broad-spectrum inhibition effect on 10 crop pathogenic fungi such as potato colletotrichum gloeosporioides, botrytis cinerea and the like, and has a very wide development and application prospect.