44 results about "Colletotrichum" patented technology

The invention discloses low-temperature-resistant rice endophytic streptomyces with biological prevention and control effects on common crop diseases and application of the low-temperature-resistant rice endophytic streptomyces, relates to the field of microorganisms, and aims to solve the problems that an existing rice blast biocontrol strain cannot play a stable effect under a low-temperature condition and is poor in rice blast control effect. The low-temperature-resistant rice endophytic streptomycete is alfalfa streptomycete F05 and is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is February 17, 2025, and the preservation number is CGMCC No.33539. The streptomyces medicaginis F05 disclosed by the invention can stably play a biological prevention and control role at a low temperature of 5-15 DEG C. The compound has broad-spectrum antibacterial performance, is used for inhibiting corn stalk rot bacteria, green bean colletotrichum, rice bakania, botrytis cinerea and rhizoctonia solani, and can also be used for decomposing starch and cellulose.

The invention provides streptomyces LZZY-S3 and application thereof, and belongs to the field of microorganisms. The streptomyces LZZY-S3 provided by the invention enriches strains for biological control, and realizes the dual functions of plant pathogenic bacterium resistance and plant growth promotion. The streptomyces LZZY-S3 has a broad-spectrum fungus inhibition function and can be applied to plant pathogenic bacterium resistance; pathogenic bacteria include: European tea leaf disease pathogen, Botryosphaeria spp., Gibberella zeae pathogen, fusarium oxysporum pathogen, wheat root rot pathogen, rice seedling rot pathogen, pumpkin gummy stem blight pathogen, wheat stem rot pathogen, Trichothecium roseum, grape white rot pathogen, and Colletotrichum graminum pathogen. The pathogenic bacteria include: European tea leaf disease pathogen, Botryosphaeria spp. Wherein the inhibition rate of tea cake disease germs reaches 91.2%. The streptomyces LZZY-S3 can also be applied to plant growth promotion, preparation of nitrogen-fixing bacterial agents, preparation and production of ACC deaminase bacterial agents and the like.

The invention relates to the field of agricultural biotechnology and plant protection, and particularly provides application of 2-heptanone to preparation of a preparation for preventing and treating plant diseases. The bactericidal composition has a strong bacteriostatic effect on litchi colletotrichum gloeosporioides, strawberry colletotrichum gloeosporioides, mango leaf blight, pawpaw colletotrichum gloeosporioides, wheat gibberellic disease, mango colletotrichum gloeosporioides, tomato fusarium wilt, banana fusarium wilt, banana colletotrichum gloeosporioides and corn root rot. The fumigant developed on the basis of 2-heptanone and used for preventing and treating phytopathogen is expected to be used for preventing and treating compound diseases caused by various pathogenic bacteria, or comprehensively preventing and controlling different crop diseases in a crop rotation system, and has important development value.

The invention discloses a single-chain alkylamino glycerol compound, a preparation method and application. The structural general formula of the single-chain alkylamino glycerol compound is R-NH-CH2-CHOH-CH2OH, the single-chain alkylamino glycerol compound is used for preventing and controlling peach monilinia and / or botryosphaeria dothidea, and R is a C8-C18 straight chain or branched chain and saturated or unsaturated alkyl. The single-chain alkylamino glycerol compound provided by the invention can be prepared by reacting glycidol and alkylamine under mild conditions, has a remarkable inhibition effect on peach monilinia and botryosphaeria dothidea, and can induce fungal membrane rupture, anti-oxidation system imbalance and lipid peroxidation, so that fungus killing is realized. The device can be widely used for fruit and vegetable postharvest preservation, orchard spraying and green pesticide development.

A new variety of garden bean (Phaseolus vulgaris L.) designated ‘Sanford’ is provided. Garden bean variety ‘Sanford’ is characterized by its dark green pods in mainly the 4 sieve class, heat tolerance, strong, erect and vigorous plant habit, and resistance to Colletotrichum lindemuthiunum (Anthracnose) and resistance to many races of Uromyces appendiculatus (Rust).

The application provides a Lecanicillium attenuatum strain and application thereof, the classification and name of the strain are LIO314, which has been preserved in the China Center for Type Culture Collection on June 18, 2025. Lecanicillium aphanocladii The address for preservation is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20251419. Research shows that the strain exhibits significant inhibitory effect on plant pathogenic fungi such as Fusarium graminearum and Colletotrichum gloeosporoides, can efficiently kill pepper aphids, has nitrogen fixation, potassium and starch decomposition activities, can significantly promote the growth of rice, wheat and lettuce seedlings, can be used as an efficient, environment-friendly and low-cost biological control agent, simultaneously has plant growth regulation function, can be used as a biological bacteria organic fertilizer, and has wide development and utilization value and application prospect in agricultural production.

The invention belongs to the technical field of microbial prevention and control of plant diseases and insect pests, and particularly discloses a plant disease biocontrol bacillus cereus strain LCBC246 which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the strain is CGMCC No.33323. The invention further discloses a preparation method of the plant disease biocontrol bacillus cereus strain LCBC246. The LCBC246 strain provided by the invention is a gram-positive bacterium, has a very strong bacteriostatic effect on colletotrichum fucicola, the bacteriostatic rate is 92.1%, the disease control rate on the colletotrichum fucicola is 90.8%, and the LCBC246 strain has a very good inhibitory effect on other important plateau characteristic crop pathogenic bacteria and also has a remarkable crop growth promoting property. The bacterial inhibition rates of fermentation liquor and volatile substances of the strain LCBC246 on colletotrichum gloeosporioides of macadamia nuts are 62.70% and 76.58% respectively; the bacillus amyloliquefaciens can generate sterilization metabolites such as cellulase, amylase and siderophore, and has a very strong broad-spectrum bacteriostatic effect on various plant pathogenic fungi. Therefore, the strain LCBC246 and the metabolite thereof have the application value of developing bacterial biopesticides.

The invention belongs to the technical field of biological medicine, discloses a bicyclic terpenoid ester compound, a preparation method and application, and particularly relates to a bicyclic terpenoid ester compound separation and purification method and alpha-glucosidase inhibitory activity of the bicyclic terpenoid ester compound, and the bicyclic terpenoid ester compound separation and purification method comprises the steps of colletotrichum fasciculatum large-scale fermentation, methanol ultrasonic extraction, vacuum concentration and ethyl acetate extraction. According to the method, the bicyclic terpenoid ester compounds in the colletotrichum fasciatum are separated and purified through the steps of normal phase column chromatography, high performance liquid chromatography separation and purification and the like, and various modern spectrum analysis technologies such as nuclear magnetic resonance spectrum, high resolution mass spectrum, infrared spectrum, ultraviolet-visible spectrum and circular dichroism spectrum are combined, so that the content of the bicyclic terpenoid ester compounds in the colletotrichum fasciatum is improved. The bicyclic terpenoid ester compound is obtained from a colletotrichum fasciculatum solid fermentation product, and the alpha-glucosidase inhibitory activity of the compound is determined. The compound is obtained by being separated from a solid rice fermentation product of a colletotrichum dematium strain, and the colletotrichum dematium strain is separated from the root part of dianthus dematium. The compound has relatively strong inhibitory activity on alpha-glucosidase, has an IC50 value of 16.8 [mu] M, and can be used for preparing hypoglycemic drugs.

The invention belongs to the technical field of plant disease prevention and treatment, and particularly relates to a polypeptide PP alpha for preventing and treating fungal diseases and application thereof. The invention discloses application of polypeptide PP alpha in inhibiting plant pathogenic fungi and preventing and treating plant diseases caused by the plant pathogenic fungi, the polypeptide PP alpha is a yeast alpha mating factor, and the amino acid sequence of the polypeptide PP alpha is shown as SEQ ID No.1. Experiments prove that the polypeptide PP alpha can significantly inhibit spore germination and appressorium formation of various plant pathogenic fungi such as pyricularia oryzae, cucurbitaceae colletotrichum spinosus, colletotrichum cryptum and botrytis cinerea, so that the infection process of the fungi is blocked. The polypeptide PP alpha provided by the invention has broad-spectrum antifungal activity, can be used as a novel green bactericide for preventing and treating fungal diseases of crops, and has a wide application prospect.

The invention relates to the technical field of molecular detection, and particularly discloses a molecular target for detecting colletotrichum ginseng. Aiming at the technical problems of long time consumption, poor specificity, difficulty in realizing accurate quantification of pathogenic bacteria in soil and the like in the existing colletotrichum ginseng detection method, the invention provides a section of specific long nucleotide sequence of colletotrichum ginseng as a molecular target, and the nucleotide sequence is shown as SEQ ID NO: 1. A specific primer group designed based on the molecular target, a kit containing the primer group and a non-diagnostic purpose detection method jointly form a complete detection system, and rapid, sensitive and specific qualitative and accurate quantitative detection of the colletotrichum ginseng in ginseng plant tissues, seeds and soil samples can be realized; and a reliable technical tool is provided for early warning of ginseng anthracnose, dynamic monitoring of pathogenic bacteria and scientific prevention and control.

The invention discloses application of 2-methylbutyric acid in prevention and treatment of postharvest diseases of mangifera indica. The diseases comprise mangifera indica anthracnose and mangifera indica stem-end rot. Wherein the pathogenic bacteria of the mango anthracnose comprise Asian colletotrichum gloeosporioides; the pathogenic bacteria of the mango stem-end rot comprise botryosphaeria dothidea and / or physosphaeria sojae. It is found for the first time that 2-methylbutyric acid has significant inhibitory activity on main pathogens after mango picking, and especially shows an efficient fumigation bacteriostatic effect on colletotrichum asiaticum causing mango anthracnose, botryosphaeria dothidea causing mango stem-end rot, and soybean mesotheca dothidea causing mango stem-end rot. 2-methylbutyric acid is mild in smell, so that the adverse effect on the original flavor of mangifera indica can be reduced to a greater extent.

This invention discloses the application of the natural product santalol as an agricultural fungicide in plant disease control, belonging to the field of plant disease prevention and control. It shows good inhibitory effects against bacterial diseases such as rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, cabbage black rot, tomato bacterial spot, kiwifruit canker, tobacco bacterial wilt, cucumber bacterial angular leaf spot, and potato bacterial wilt. It also shows good inhibitory effects against viral diseases such as tobacco mosaic virus; and against fungal diseases such as rice rhizome blight, wheat scab, pepper wilt, *Botrytis cinerea*, or *Colletotrichum sorghum*. It can be used in the preparation of plant fungicides. Santalol is a natural plant extract and is environmentally friendly. This invention provides a new use for the natural product santalol, enabling its application in the agricultural field for plant disease control and providing a new avenue for the research and development of new pesticides.

The invention provides a lecanicillium lecanii strain and application thereof, the classification name of the lecanicillium lecanii strain is Lecanicillium aphanocladiii LI0314, and the lecanicillium lecanii strain is preserved in the China Center for Type Culture Collection on June 18, 2025. The preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20251419. Researches show that the strain has a remarkable inhibition effect on plant pathogens such as fusarium graminearum and colletotrichum laevis, can efficiently kill pepper aphids, has nitrogen fixation, potassium decomposition and starch decomposition activities, can remarkably promote growth of seedlings of rice, wheat, lettuce and the like, and has a good application prospect. The strain can be used as a high-efficiency, environment-friendly and low-cost biological control microbial agent, has a plant growth regulation function, can be used as a biological bacterial organic fertilizer, and has wide development and utilization values and application prospects in agricultural production.

The application discloses a strain of Streptomyces clyindrosporus 11-7 and application thereof, and belongs to the technical field of microorganisms. The strain of Streptomyces clyindrosporus 11-7 disclosed by the application has a preservation number of CCTCC NO: M 2025222. The strain of Streptomyces clyindrosporus 11-7 disclosed by the application has the best inhibitory activity on Colletotrichum chlorophyti, Colletotrichum plurivorum and Rigidoporus lignosus, and the inhibitory rates are 96.75%, 95.89% and 92.94% respectively. The volatile substances produced by the strain of Streptomyces clyindrosporus 11-7 have inhibitory effects on various pathogenic bacteria. The strain of Streptomyces clyindrosporus 11-7 can fix nitrogen, produce ammonia and produce indole acetic acid.

The invention discloses streptomyces fulvus 11-7 and application thereof, and belongs to the technical field of microorganisms. The invention discloses a streptomyces fulvus strain 11-7, and the preservation number of the streptomyces fulvus strain 11-7 is CCTCC (China Center for Type Culture Collection) NO: M 2025222. The streptomyces fulvus 11-7 disclosed by the invention has the optimal bacteriostatic activity on Colletotrichum chlorophyti, Colletotrichum plurivorin and Rigidoporus ligosus, and the bacteriostatic rates of the streptomyces fulvus 11-7 are respectively 96.75%, 95.89% and 92.94%. The streptomyces fulvus 11-7 disclosed by the invention has the advantages that the streptomyces fulvus 11-7 can be used for preparing the antibacterial agent for preventing and treating the pathogenic bacteria of the Colletotrichum chlorophyti, the Colletotrichum plurivorin and the Rigidoporus ligosus; volatile substances generated by the streptomyces fulvus 11-7 have an inhibition effect on a plurality of pathogenic bacteria. The streptomyces fulvus 11-7 can be used for fixing nitrogen, producing ammonia and producing indoleacetic acid.

The invention discloses an in-vitro high-throughput inoculation method of tea tree colletotrichum gloeosporioides, and belongs to the technical field of tea tree biology. The method comprises the following steps: preparing a spore suspension with the concentration gradient (absorbance) OD600 of 0.4, 0.6, 0.8 and 1.0 from the Colletotrichum calamiae, inoculating the spore suspension to an in-vitro tea tree leaf in an injection manner, then placing the leaf in a high-throughput microenvironment culture device, and culturing for 4 days under the conditions of 28 DEG C, 75% relative humidity and 12h illumination / 12h darkness, thereby accurately evaluating the infection efficiency of the pathogenic bacteria. According to the method, a standardized inoculation process is combined with high-throughput microenvironment control, so that the problems of long period, non-uniform environment and low efficiency of a traditional method are solved, and the method has the outstanding advantages of short inoculation period, good experimental result consistency and capability of realizing batch high-throughput screening; the method can be directly used for tea tree colletotrichum gloeosporioides resistance identification, pathogen toxicity analysis and bactericide screening.

The invention belongs to the technical field of biological agents, and particularly relates to trichoderma harzianum, a biocontrol agent, a bio-organic fertilizer, a method for preventing and treating plant diseases and application. The invention provides a trichoderma harzianum P11 with the preservation number of CCTCC NO: M 2024648. The trichoderma harzianum P11 can improve the relative expression quantity of disease-resistant positive correlation genes PnCAD, PnSN1 and PnDEFL1 in plants and improve the activity of disease-resistant related enzyme activities SOD, POD and CAT, and the trichoderma harzianum P11 can damage cell membranes of pathogenic bacteria and inhibit the growth of the disease pathogenic bacteria. The trichoderma harzianum P11 has good rhizosphere colonization capability. The result of the embodiment shows that the trichoderma harzianum P11 can reduce the morbidity of panax notoginseng field root rot, and can also antagonize ginseng alternaria alternata, magnaporthe oryzae, polygonatum kingianum colletotrichum, geotrichum candidum, alternaria solani, cylindrosporium destroyed, phytophthora capsici and conidiospora spiraea.

The invention discloses a method for biosynthesizing nano platinum by using space mutant bacteria PS04-17 and application of the method. The invention provides a novel application of a space mutant strain PS04-17 strain in biosynthesis of nano platinum particles. Researches show that the PS04-17 strain has a reduction capability on hexachloroplatinic acid; the method for biologically synthesizing nano platinum by reducing hexachloroplatinic acid in a liquid fermentation culture medium by using the PS04-17 strain is simple and convenient, mild in condition, low in cost and environment-friendly. The invention provides more new strain resources with high reduction efficiency on hexachloroplatinic acid, and fills the blank in the field of synthesis of nano platinum by microorganisms. Meanwhile, the PS04-17 synthesized nano platinum can prevent and control various plant pathogens, the bacteriostasis rate of the PS04-17 synthesized nano platinum on colletotrichum litchii and peronophythora litchii can reach 100%, the PS04-17 synthesized nano platinum can also be used for preventing and controlling wheat stem rot, the prevention and control efficiency reaches 78.2%, and a new solution is provided for prevention and control of crop diseases.

The invention belongs to the technical field of biology, and particularly relates to a microbial marker combination for evaluating the quality of polygonatum sibiricum and application, the microbial marker combination comprises one or more of Aspergillus, Cercophora, Escherichia. Shigilla, Mycoplasm, Muribacaceae, Pseudomonas, Colletotrichum and Asaia, a method and an evaluation model for evaluating the quality of polygonatum sibiricum are established by utilizing the microbial marker combination, the sensitivity is 100.0%, the specificity is 72.7%, the AUC is 89.8%, and the evaluation model is used for evaluating the quality of polygonatum sibiricum. The method has high accuracy, specificity and sensitivity, and the evaluation method is economical, practical and easy and convenient to operate and can be widely applied to quality evaluation of rhizoma polygonati.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus velezensis and application thereof. The bacillus velezensis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC 25698. The strain has a strong specific antagonistic effect on streptomyces scabies X-1 (Streptomyces scabies X-1), the prevention effect of a pot experiment reaches 70.90%, and the strain has no pathogenic risk on potato tubers. A further research shows that the bacillus velezensis XM18-5 has a broad-spectrum inhibition effect on 10 crop pathogenic fungi such as potato colletotrichum gloeosporioides, botrytis cinerea and the like, and has a very wide development and application prospect.

The present invention provides the use of banana root exudates or ethanol extracts of banana root exudates in the preparation of 10-hydroxydecanoic acid. By inducing changes in banana root exudates with a newly isolated Streptomyces sp., the key root exudate 10-hydroxydecanoic acid can be isolated, which can effectively antagonize Gibberella zeae and others, but has no inhibitory effect on Fusarium oxysporum f. sp. cubense race 4 and others. However, 10-hydroxydecanoic acid can effectively inhibit Fusarium oxysporum f. sp. cubense race 4 in the rhizosphere soil of banana and effectively control banana wilt caused by Fusarium oxysporum f. sp. cubense race 4, and increase the Sphingomonas abundance of genera Bacills of bacteria Pseudolabrys and others in the rhizosphere soil of banana. The present invention provides new methods and ideas for the prevention and control of wilt diseases and the control of Gibberella zeae, Colletotrichum gloeosporioides, Colletotrichum musae, Colletotrichum litchii, Colletotrichum gloeosporioides, Botrytis cinerea and others.

The invention belongs to the technical field of microorganism and plant disease control, and discloses a bacillus velezensis strain YNDH2404, the preservation number of which is CCTCC (China Center for Type Culture Collection) NO: M 20242825, and the preservation number of which is CCTCC NO: M 20242825. The invention further discloses application of the bacillus velezensis or fermentation liquor thereof. Experiments prove that the thalli and fermentation products of the bacillus velezensis, which are separated and identified by the invention, have a remarkable inhibition effect on coffee lacquer blotch or konjac lacquer blotch caused by pseudomonas brachytrichum, coffee anthracnose caused by colletotrichum gloeosporioides and coffee stem rot caused by fusarium solani. The microbial inoculum prepared from the biocontrol bacterium does not cause pollution to the ecological environment in the use process, is nuisanceless, can reduce the dosage of other chemical pesticides, reduces the prevention and control cost of coffee and konjak diseases, provides guarantee for making'green food brand 'of coffee and konjak, and has good popularization value.

The present invention belongs to the field of microorganisms, and specifically relates to a banana endophyte compound agent, a preparation method, and an application thereof. The present invention isolates a novel endophytic Bacillus Velez JDB15 and an endophytic Trichoderma harzianum JDL4 from banana plants. The Trichoderma harzianum JDL4 can inhibit Botrytis cinerea, Fusarium graminearum, Rhizoctonia solani, Colletotrichum syringae prunellae, Peach anthracnose, Peach brown rot, and Alternaria alternata, and has a good broad-spectrum antibacterial effect. At the same time, the filtrate of the fermentation broth of Trichoderma harzianum JDL4 is compounded with Bacillus Velez JDB15 to significantly enhance the antibacterial effect, and the control effect against banana wilt pathogen, rice blast pathogen, tomato wilt, and corn leaf spot pathogen is also significantly enhanced. The compound agent has a wide range of biocontrol activities and can be further developed in the future to provide a sustainable and green solution for the control of banana wilt and other plant diseases.

The present application relates to the technical field of pharmaceutical chemistry, and particularly relates to an alpha, beta-unsaturated amide or hydrazide compound containing a carbonyl structure, a preparation method and application. The present application mainly prepares the alpha, beta-unsaturated amide or hydrazide compound containing a carbonyl structure. The compound has a novel structure, and has a good inhibitory effect on plant pathogenic fungi, and particularly has a good inhibitory effect on plant pathogenic fungi such as grape colletotrichum, rice sheath blight, brassica sclerotinia, wheat scab, tomato early blight, tobacco brown spot and eggplant fusarium wilt. The compound has outstanding advantages in the prevention and treatment of plant fungal diseases.

The application relates to the field of agricultural biotechnology and plant protection, and specifically provides application of 2-heptanone in preparation of a preparation for preventing and treating plant diseases, the 2-heptanone has broad-spectrum bacteriostasis, and has strong bacteriostasis on colletotrichum gloeosporioides, colletotrichum acutatum, mycosphaerella musicola, colletotrichum caricae, fusarium graminearum, colletotrichum musae, fusarium solani, fusarium oxysporum, colletotrichum musae, and fusarium verticillioides. The fumigant for preventing and treating plant pathogens developed based on the 2-heptanone is expected to be used for preventing and treating complex diseases caused by various pathogens, or comprehensively preventing and controlling different crop diseases in a crop rotation system, and has important development value.

The invention discloses bacillus velezensis SGTSH 1011, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.34265. The invention further discloses a microbial pesticide preparation which comprises the bacillus velezensis SGTSH 1011 parent drug, a dispersing agent, an emulsifying agent, a stabilizing agent, a thickening agent and a dispersing medium. The invention also discloses application of the microbial pesticide preparation in prevention and treatment of sunflower sclerotiniose. The strain has remarkable inhibition activity on sunflower sclerotinia sclerotiorum pathogenic bacteria sclerotinia sclerotiorum, the inhibition rate reaches up to 85.68%, meanwhile, the strain shows a broad-spectrum antibacterial effect on various plant pathogenic fungi such as sorghum colletotrichum, fusarium oxysporum, fusarium graminearum and alternaria solani, and good biocontrol application potential is achieved. By optimizing a preparation formula, the technical problem that a living microbial preparation is easy to inactivate in the storage and transportation processes is solved.

The present application relates to the technical field of molecular detection, and specifically discloses a molecular target for detecting gongronella panacis. In view of the technical problems of long time consumption, poor specificity and difficulty in realizing accurate quantification of pathogenic bacteria in soil existing in the current detection method of gongronella panacis, the present application provides a long nucleotide sequence specific to gongronella panacis as a molecular target, and the nucleotide sequence is shown as SEQ ID NO:1. The specific primer set designed based on the molecular target, the kit containing the primer set and the detection method for non-diagnostic purposes jointly constitute a complete detection system, which can realize rapid, sensitive, specific qualitative and accurate quantitative detection of gongronella panacis in ginseng plant tissues, seeds and soil samples, and provide a reliable technical tool for early warning, dynamic monitoring and scientific prevention and control of ginseng anthracnose.

The invention discloses a bacillus Bwzl 19 strain and an application of the bacillus Bwzl 19 strain. The bacillus sp. Bwzl 19 strain is separated from tobacco leaves, is preserved in Guangdong Microbial Culture Collection Center on November 8, 2024, has a preservation number of GDMCC NO: 65445, and is a new species of bacillus if the consistency of the whole genome average nucleotide of any reference strain in the bacillus does not reach a threshold value (gt, 95%) for judging that the reference strain is the same species. The Bwzl 19 strain does not carry virulence genes, can rapidly grow with starch as a unique carbon source and is high in nicotine tolerance, the content of starch in tobacco leaves can be reduced by spraying a fermentation culture solution of the Bwzl 19 strain to the tobacco leaves, and the Bwzl 19 strain can be used for improving the quality of the tobacco leaves. In addition, the Bwzl 19 strain can also effectively inhibit plant pathogenic fungi such as colletotrichum hijinensis and the like, and can be prepared into a microbial agent for preventing and treating related plant fungal diseases.

The invention provides bacillus vallismortis TCS004 with a preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.25850. The invention discloses an application of the bacillus vallismortis TCS004 in preventing and treating plant pathogenic fungi or plant pathogenic bacteria, or an application of the bacillus vallismortis TCS004 in preparing a microbial preparation for preventing and treating the plant pathogenic fungi or the plant pathogenic bacteria. The bacterial strain has a very high inhibition effect on plant pathogenic fungi such as lasiodiplodia mali, macrostem botryosphaeria verticillata, citrus interspersonia, streptosclerotinia sclerotiorum, alternaria alternata, colletotrichum frutescens, muscardine fungus, alternaria solani, fusarium solani, botrytis cinerea, fusarium equisetum, curvularia, alternaria alternata and the like; and the compound also has relatively good bacteriostatic activity on plant pathogenic bacteria xanthomonas oryzae and the like. The invention further provides a formula of a fermentation culture medium of the bacillus vallismortis TCS004 and fermentation process conditions of the bacillus vallismortis TCS004.

The application discloses a benzopentaheterocyclic compound containing a hydrazine structure and an application thereof. The compound, stereoisomers of the compound, or a salt of the compound, or a solvate of the compound has good inhibiting effects on plant pathogenic bacteria and fungi. The compound has good inhibiting effects on fungi such as Colletotrichum gloeosporioides, Colletotrichum theae-sinensis, Mycosphaerella pachyrrhizi, Alternaria solani, Phytophthora capsici and the like, and pathogenic bacteria such as Xanthomonas oryzae, Xanthomonas citri and the like. Some of the compounds show excellent inhibiting activities on Colletotrichum theae-sinensis, with an EC 50 of 11.85 μg / mL. Some of the compounds show excellent inhibiting activities on Mycosphaerella pachyrrhizi, with an EC 50 of 2.88-16.41 μg / mL. Some of the compounds show excellent inhibiting activities on Xanthomonas citri, with a primary screening activity of 13.69-27.03 μg / mL, which is superior to a commercial drug, copper oxychloride. Some of the compounds show excellent inhibiting activities on Xanthomonas campestris, with a primary screening activity of 25.07-43.78 μg / mL, which is superior to the commercial drug, copper oxychloride. The application provides an important scientific basis for the research and development of new pesticides.