107 results about "Colletotrichum" patented technology

This invention relates to polynucleotide sequences encoding a gene that can confer resistance to the plant pathogen Colletotrichum, which causes anthracnose stalk rot, leaf blight and top dieback in corn and other cereals. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to the plant pathogen Colletotrichum, and processes of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently introgress the gene from corn lines carrying said gene into other corn lines that do not carry said gene, in order to make them resistant to Colletotrichum and resistant to stalk rot.

This invention relates to polynucleotide sequences encoding a gene that can confer resistance to the plant pathogen Colletotrichum, which causes anthracnose stalk rot, leaf blight and top dieback in corn and other cereals. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to the plant pathogen Colletotrichum, and processes of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently introgress the gene from corn lines carrying said gene into other corn lines that do not carry said gene, in order to make them resistant to Colletotrichum and resistant to stalk rot.

The present invention relates to an application of anthraquinone derivative as pesticice for controlling plant fungous diseases. The anthraquinone derivative pesticide contains one kind or several kinds of 10 anthraquinone derivative compounds. The anthraquinone derivative compounds have obvious action for preventing and curing several plant diseases, and can be used for preventing and curing economic crop, grain crop, vegetable, fruit tree and melon diseases due to fungi, including sphaerotheca, erysiphe, blumeria, fusarium, sclerotinia, pyricularia, rhizoctonia, colletotrichum, botrytis, verticillium and alternaria.

Systems are provided for preventing post harvest fungal diseases of food systems, such as but not limited to fresh produce, such as but not limited to berries (e.g., blueberries). For example, various anti-fungal compounds can incorporated or encapsulated into cyclodextrins, such as but not limited to α, β and/or γ cyclodextrins. The encapsulated anti-fungal materials can be used alone (e.g., brought into proximity to the produce) or incorporated into film and/or packaging materials that are used in the packing and/or storing of produce. By way of a non-limiting example, the anti-fungal compounds can include volatile compounds such as but not limited to acetaldehyde, hexanal and 2E-hexenal. The cyclodextrins provide controlled release of the volatiles over a period of at least several days such that they prevent or inhibit fungal growth, including but not limited to several species of the Colletotrichum, Altermaria, Botrytis, Penicillium and/or Aspergillus genera.

The invention discloses a pseudomonas aeruginosa D10 which is preserved in China Typical Culture Preservation Center, and the preservation number is CCTCC NO: M 209104. A colony on a beef paste peptone culture medium appears brown, round, glossy and wet, and has trim edges; the diameter of the colony is 0.2-0.5 cm; and the thallus is in a short bar shape and does not have gemma. The invention also discloses a preparation method and applications of the pseudomonas aeruginosa D10. The pseudomonas aeruginosa D10 of the invention can inhibit corn northern and southern leaf blight, has strong antergic functions on corn northern leaf blight, corn southern leaf blight, corn curvalaria leaf spot, cotton verticillium wilt, wheat fusarium graminearum, banana colletotrichum toilette and banana fusarium wilt, has the advantages of wide bacteriostatic range, high prevention efficiency and favorable environmental safety, and has favorable prospects for development and applications.

The invention discloses a method for storage and fresh preservation of litchi fruits. The litchi fruits are soaked into 10 to 140 mg/L aqueous solution of chlorine dioxide and then are sealed, stored and preserved fresh. The method determines the proper concentration of the chlorine dioxide by screening different concentrations of the chlorine dioxide so as to effectively inhibit the growth of various fungi and bacteria after the litchi fruits are picked and obviously inhibit the germination of colletotrichum spores of the litchi fruits; by combining optimal storage conditions and methods forthe fresh-preservation treated litchi fruits, the method effectively delays the ageing symptom of the storage process after the litchi fruits are picked, obviously improves the quality of the stored fruits, and prolongs the storage period; and according to the treatment method, the rotting rate of the picked litchi fruits can be remarkably reduced, the flavor of pulp is kept, and the litchi fruits nearly have no medicinal residue and reach the health quarantine standards of European and American imports and exports.

The invention discloses a strain of Paenibacillus jejuni for inhibiting anthrax and Fusarium and an application thereof. A strain of Paenibacillus jamilae is isolated from the deep sea sediment, the strain was deposited at the General Microbial Center of the China Microbial Culture Collection Management Committee under accession number CGMCC No.16231 on August 8, 2018. The strain has broad-spectrum resistance to many plant pathogens, and has good control effect on fruit tree (citrus, apple, grape) anthracnose caused by Colletotrichum gloeosporioides and vegetable wilt caused by Fusarium oxysporum.

The invention discloses a bacillus amyloliquefaciens and a microbial inoculum, and a production method and application of the microbial inoculum, and belongs to the field of microorganisms. The bacillus amyloliquefaciens (CGMCC (China General Microbiological Culture Collection Center) No.13047) has the disease prevention and growth promoting capability. The bacillus amyloliquefaciens with biological activity is inoculated in a solid substrate to be fermented; an obtained fermentation product can be directly used as a bacillus amyloliquefaciens microorganism inoculants; the microbial inoculum has the bacteriostasis effect and can be used as a microbial pesticide; phoma foveata, colletotrichum coccodes, fusarium avenaceum and fusarium chlamydosporum can be inhibited; the bacteriostasis rate is 67 percent or higher, so that the defects of poor environmental protection property, easy generation of drug resistance and poor safety of the chemical pesticide in the prior art can be overcome; the advantages of good environmental protection property, no drug resistance and high safety are realized.

The invention discloses Bacillus amyloliquefaciens subsp. plantarum. The classified nomenclature of the Bacillus amyloliquefaciens subsp. plantarum is Bacillus amyloliquefaciens subsp. plantarum B203; the Bacillus amyloliquefaciens subsp. plantarum was collected in China General Microbiological Culture Collection Center on May 25, 2012; and the collection number is CGMCC No. 6156. The Bacillus amyloliquefaciens subsp. plantarum is mainly used for biological control of strawberry anthracnose. The Bacillus amyloliquefaciens subsp. plantarum B203 has a remarkable inhibitory effect on Colletotrichum fragaliae Brooks, strawberry Botrytis cinerea and various pathogenic fungi of poplar canker, and the antagonistic bacterium also has a good control effect on various other plant diseases; the propagation speed is high; the Bacillus amyloliquefaciens subsp. plantarum can be manually cultured, is easy to operate, and can be conveniently produced and applied; the Bacillus amyloliquefaciens subsp. plantarum has the advantages of high stress resistance and the like, and has an important significance for controlling the strawberry anthracnose caused by fungi; and culture conditions are simple, and the Bacillus amyloliquefaciens subsp. plantarum is easy to preserve, can be industrially produced easily, and has a good development and application prospect.

The invention mainly provides a thiosemicarbazone compound which has an inhibition effect on fusarium oxysporum, fusarium moniliforme, fusarium solani and colletotrichum and a preparation method thereof. The chemical structural formula of the compound is shown in the specification. According to the invention, a novel way for preventing and controlling plant diseases caused by fusarium oxysporum, fusarium moniliforme, fusarium solani and colletotrichum is provided; and the preparation method is simple and convenient and has strong operability.

The invention discloses a huperzine A high-producing strain and a method for producing huperzine A by fermenting the same, belonging to the technical field of biology. The huperzine A high-producing strain is Colletotrichum gloeosporioidesisolate YLJ-13 preserved in the China Center for Type Culture Collection on July 17, 2010, and the preservation number is CCTCC No. M2010181. The strain provided by the invention is separated and purified from huperzia crispate through pressure screening, and the strain is identified as a new variety of Colletotrichum gloeosporioidesisolate through a molecular biological means. The strain is fermented in an improved potato liquid culture medium, and huperzine A is obtained from the fermentation liquid. Huperzine A obtained through fermentation production can be secreted to be out of cell, the improved potato liquid culture medium is used as a precursor of biological transformation, and up to 199.6 mg/L huperzine A can be obtained from each liter of culture system. The strain provided by the invention is used for producing huperzine A. The existing rare medicinal huperziaceae plant resources can be protected, but also the shortage situation of huperzine A medication demand can be alleviated.

The invention belongs to the field of biocontrol strain excavation and utilization, in particular to bacillus subtilis Baisha2C for restraining plant pathogenic fungi, and the preservation number of the bacillus subtilis Baisha2C is CGMCC No.10963. The invention further discloses molecular biological characteristics, physiological-biochemical characteristics and a cultivation method of the bacillus subtilis Baisha2C, and the bacillus subtilis Baisha2C has activity for restraining various kinds of the plant pathogenic fungi, such as fusarium colletotrichum, colletotrichum gloeosporioides and fusarium.

The invention discloses an application of a volatile substance produced by streptomyces fimicarius in control of plant diseases. Particularly, the volatile substance produced by streptomyces fimicarius can inhibit growth of 15 phytopathogens, has the highest inhibition function on peronophythora litchii, pythium myriotylum, alternaria alternate, fusarium graminearum, colletotrichum musarum and pyricularia grisea and has the inhibition rate up to 100%. The invention further makes clear that the volatile substance produced by streptomyces fimicarius contains 7 compounds with remarkable bacteriostasis, and a direction is provided for future studies of control drugs for peronophythora litchii, pythium myriotylum, alternaria alternate, colletotrichum musarum, pyricularia grisea and other phytopathogens.

The invention relates to bacillus substilis BS01 and an inoculant thereof, as well as application of the bacillus substilis BS01 to inhibition of picked fruit pathogenic bacteria. The bacillus substilis BS01 is preserved in Microbial Culture Preservation Center, Guangdong Province (GDMCC) on August, 29, 2016, and the preservation number is GDMCC NO.60069. The in-vitro antibacterial activity of the supernatant of the fermenting liquid of the bacillus substilis BS01 strain provided by the invention on the picked fruit pathogenic bacteria, particularly colletotrichum, can reach 90 percent or above, and is about three times of the antibacterial activity of the supernatant of the fermenting liquid generated by fermentation of the common LB culture medium before optimization; and the inhibition effect of the fermenting liquid of the BS01 strain obtained by the fermentation condition after optimization on anthracnose, such as banana anthracnose, tomato anthracnose and avocado anthracnose, can reach 65 percent or above.

The invention discloses Streptomyces sp.B5017. The Streptomyces sp.B5017 is characterized in that the preservation number is CCTCC NO: M2019648, and the Streptomyces sp.B5017 is classified and named as Streptomyces sp.B5017. Tests show that the marine-derived streptomyces new strain disclosed by the invention has the effect of antagonizing various phytopathogens, and has an inhibiting effect on gloeosporium musarum, colletotrichum gloeosporioides, colletotrichum pearl plum, botryosphaeria dothidea, fusarium oxysporum f.sp.cubense race 4, fusarium oxysporum f.sp.cubense race 1 microspecies or alternaria alternata. Fermentation liquor prepared from the Streptomyces sp.B5017 disclosed by the invention can effectively avoid withered and yellow withering of leaves caused by the fusarium oxysporum f.sp.cubense race 4 and race 1 in potting experiments, which proves that the Streptomyces sp.B5017 is capable of effectively preventing and treating banana wilt and enhancing the disease resistanceof bananas, has good ecological benefits and social benefits, and has a good application prospect when being developed as a biocontrol agent.

The invention relates to the technical field of biological prevention and control and bioengineering, in particular to bacillus amyloliquefaciens SY-SF-01 and application thereof. Bacillus amyloliquefaciens SY-SF-01 is preserved in China Center for Type Culture Collection, the preservation number is CCTCC M 2018264, and the preservation time is May fourteenth, 2018. The invention further providesa screening method of bacillus amyloliquefaciens SY-SF-01 and application of bacillus amyloliquefaciens SY-SF-01 in inhibition of rhizoctonia solani, cucumber fusarium wilt, thanatephorus cucumeris, red bean colletotrichum and verticillium dahliae kleb. A bacillus amyloliquefaciens SY-SF-01 strain has an extremely obvious prevention and control effect on rhizoctonia solani, the prevention rate forliving bodies reaches above 90%, the strain also has a good prevention and control effect on thanatephorus cucumeris, and the in-vitro antibacterial rate and the prevention rate for living potted plants can reach above 90%.

The invention provides a Bacillus mojavensis QLY002 strain. The Bacillus mojavensis QLY002 strain has a preservation number of CGMCC No. 8905. The invention also provides a microbial inoculum of the Bacillus mojavensis QLY002 strain and its preparation method and use in potato pathogen inhibition and potato growth promotion. The Bacillus mojavensis QLY002 strain and the microbial inoculum can be used as microbe pesticides and fertilizer, can inhibit Phoma foveata, Alternaria solani, Fusarium oxysporum and Colletotrichum coccodes, have an antibacterial rate more than 61%, have potato growth promotion effects, can be used as potato special-purpose microbial pesticide and microbial fertilizer, solve the problem that the existing chemical pesticide has poor environmental friendliness, easily causes pesticide resistance and has poor safety, and have the advantages of good environmental friendliness, no pesticide resistance and good safety.

The invention is a streptomyces angustmyceticus ER1396 strain (having the preservation number of CGMCC No.9871 in CGMCC) and a method for preparing a soluble powder from a fermented liquid. The soluble powder can effectively prevent and control anthracnose caused by colletotrichum spp. fungus invasion.

The invention relates to a preparation method of a novel medicine for inhibiting Cylindrocladium parasiticum, Colletotrichum corda and Phomopsis(Sacc.)Bubak. Indoleacetic acid, bromoethanol and carbendazim active compounds are used as synthetic raw materials to prepare an indoleacetic acid-carbendazim conjugate, which is the finish product, through a reaction. The invention also discloses nuclear magnetic resonance structure data, a hydrogen spectrogram and a carbon spectrogram of the novel compound. The compound has excellent antibacterial effects on the Cylindrocladium parasiticum, the Colletotrichum corda and the Phomopsis(Sacc.)Bubak.

The invention relates to the field of agricultural pharmacology and organic chemistry, and discloses a sterilizing agent containing 1, 3, 4-oxadiazole cyclodistyrene amide as well as a preparation method and application thereof. The sterilizing agent has the structure shown in the formula (I). The sterilizing agent has the characteristic of high efficiency and low toxicity, and has a good prevention and treatment effect on botrytis cinerea and colletotrichum. The formula (I) is shown in the description.

The invention discloses a soil fumigant compounded of dimethyl disulfide, nitrochloroform and abamectin and application of the soil fumigant, belongs to the technical field of pesticides for soil. The soil fumigant comprises the following active ingredients in weight percent: 38%-70% of dimethyl disulfide, 10%-60% of nitrochloroform and 0.1%-2% of abamectin. The soil fumigant provided by the invention is capable of effectively preventing and controlling soil borne pathogenic fungi, nematode and weeds and is broad in spectrum. The soil fumigant provided by the invention has obvious synergism to root-knot nematode, fusarium, phytophthora fungi, pythium fungi, basidiomycota fungi, colletotrichum fungi, cylindrocarpon fungi, pyrenochaeta fungi, rhizoctonia fungi and verticillium fungi and field weeds, and is convenient to apply and safe to crops; meanwhile, three chemicals have no residue in soil and are safe to crops.

The invention discloses a method of efficiently catalyzing dehydroepiandrosterone by utilization of a resting cell of colletotrichum linli ST-1. The method combines resting cell conversion and substrate assisted solubilization, and increases the utilization rate of a substrate and the product yield. A sodium phosphate buffer provides a stable pH environment for the conversion reaction. Tween 80 can assist solubilization and promote thallus growth. By utilization of a novel conversion process, the molar product yield is increased by 78.3% than that of traditional conversion methods. The conversion rate of the product in the method is 90.4% while the conversion rate of the product of the traditional methods is 67.3%.

A gene for testing the resistance of colletotrichum gloeosporiodies to carbendazim and its test reagent kit are disclosed. Said gene is the beta-microtubulin gene fragment of Colletotrichum gloeosporiodies. Said reagent kit is composed of 10XPCR buffering liquid, MgCl2 solution, dNTP (Mixture), upstream primers CGR1 and CGS2, and downstream primers CGrev and TaqDNA polymerase. Its advantages are high speed, high correctness (95.71%) and high sensitivity.

The invention discloses an application of two gliotoxins in the preparation of a biopesticide. The compounds 6-deoxy-5a, 6-didehydrogliotoxin, 5a, 6-didehydrogliotoxin have an obvious inhibition effect on plant pathogenic fungi colletotrichum gloesporioides and alternaria alternate. The compounds 6-deoxy-5a, 6-didehydrogliotoxin, 5a, 6-didehydrogliotoxin have an obvious plant pathogenic fungi inhibition effect and have good development and application prospects. Therefore, a candidate bio-pesticide is provided for research and development of novel plant pathogenic fungi and a scientific basis is provided for the development and utilization of natural active materials with a marine fungus source.

The invention discloses a strain of euonymus japonicus endophytic bacterium bacillus methylotrophicus HYEB 5-6 which was preserved in China Center for Type Culture Collection on Dec 8th, 2015, and the preservation serial number is CCTCC No: M2015728. The bacillus methylotrophicus HYEB 5-6 is an euonymus japonicus endophytic bacterium and can delay germination of euonymus japonicus anthrax conidium, inhibit extension of a germ tube, inhibit germination of the conidium, induce hypha to generate an expanded body and cause malformation of the hypha; it is proved by inoculation experiment results that after HYEB 5-6 thallus is sprayed, the index of decease infection of euonymus japonicus anthracnose is remarkably inhibited, and scabs of euonymus japonicus anthracnose are reduced; the HYEB 5-6 strain has a certain antagonistic effect on euonymus japonicus colletotrichum, alternaria spp, cytospora chrysosperma, higgins dish bacteria, magnaporthe oryzae, rhizoctonia, horse chestnut fusicoccin and fusarium solani.

The invention relates to a method of isolating and screening antagonistic bacteria from rubber tree leaves. According to the method, bacteria are isolated from diseased leaves by a conventional sampling isolation method, and a strain of the antagonistic bacteria with an obvious inhibition action on the anthracnose pathogen RC178 of rubber trees is obtained by carrying out confront culture on the bacteria and test bacteria colletotrichum RC178 of the rubber trees. The isolated antagonistic bacteria are usually used as biocontrol bacteria and expected to be prepared into a microbial preparation, and therefore a basic preparation is provided for biocontrol of anthracnose of the rubber trees.