12 results about "Puccinia" patented technology

The invention provides application of a TaNAC42 gene in regulation and control of wheat stem rust resistance, and belongs to the technical field of gene functions. It is found for the first time that the TaNAC42 gene of wheat is related to the wheat stem rust resistance, the TaNAC42 gene is induced to be silenced through viruses, the wheat stem rust resistance can be enhanced, wheat plants are easier to resist diseases, the TaNAC42 gene is over-expressed through the agrobacterium tumefaciens-mediated transient over-expression technology, the wheat stem rust resistance can be weakened, the wheat plants are more susceptible to diseases, and the wheat plants are more susceptible to diseases. Namely, the TaNAC42 gene can negatively participate in regulating and controlling the resistance of the wheat to the Puccinia graminicola, a new target gene is provided for improved breeding of wheat Puccinia graminicola resistance molecules, and the TaNAC42 gene has important utilization value in wheat production and breeding.

The application discloses a wheat disease-resistant gene TaLOX and application thereof in regulating resistance to stripe rust. TaLOX The application overexpresses the wheat gene in wheat, inoculates the overexpressed wheat plants with stripe rust, and observes the resistance phenotype of the overexpressed wheat plants to the stripe rust (Puccinia striiformis f. Puccinia striiformis sp. tritici ). TaLOX The results show that, compared with a control group, the amount of spores of the stripe rust on the surface of the overexpressed wheat plants is significantly reduced, the disease index and the fungal biomass are significantly reduced, and the mycelium length is significantly shorter than that of the control group, indicating that the gene positively regulates the resistance of the wheat to the stripe rust. TaLOX The application has application prospects in breeding of wheat varieties resistant to stripe rust and improvement of the resistance level of the wheat to the stripe rust.

The invention discloses application of TaSCL6-A1 protein and a coding gene thereof in regulating and controlling leaf rust resistance of wheat. The invention provides an application of TaSCL6-A1 protein or a coding gene thereof in regulating and controlling the resistance of wheat to leaf rust (fungus). The invention proves that after the TaSCL6-A1 is over-expressed in the wheat, the resistance of the wheat to the puccinia repens is obviously improved, and after the TaSCL6-1 is knocked out, the resistance of the wheat to the puccinia repens is obviously reduced, which indicates that the TaSCL6-A1 positively regulates and controls the leaf rust resistance of the wheat. According to the invention, wheat molecular marker-assisted disease-resistant breeding work is enhanced, and resistance genes with different disease-resistant mechanisms are subsequently optimized, selected, edited and polymerized, so that sustainable leaf rust resistance is enhanced, and cultivation of a new leaf rust-resistant wheat variety is accelerated.

The invention belongs to the technical field of microorganisms, and particularly relates to a strain of trichothecium roseum T181 for preventing and treating blister rust of Huangshan and application of the trichothecium roseum T181. The trichothecium roseum T181 disclosed by the invention is preserved in Guangdong Microbial Culture Collection Center on August 1, 2025, the preservation address is No.59, Courtyard, No.100, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCC No: 66796. The trichothecium roseum T181 disclosed by the invention can effectively inhibit the normal physiological and biochemical state of puccinia armandii rust spores, can be used as a biocontrol bacterium for the puccinia armandii, and has a wide application prospect in biological prevention and control of the puccinia armandii.

The invention relates to a bicyclooctane-1, 2, 4-oxadiazole derivative as well as a preparation method and application thereof, and belongs to the technical field of pesticides. The structure of the bicyclooctane-1, 2, 4-oxadiazole derivative is as shown in a formula I in the specification, the invention also provides a method for preparing the compound as shown in the formula I and application of the compound. The dicyclooctane-1, 2, 4-oxadiazole derivative provided by the invention has a remarkable inhibition effect on puccinia puccinia under the condition of low application amount, and has an inhibition rate of 80% or more on puccinia puccinia when the low concentration is 1.6 mg / L, and the inhibition effect is obviously superior to that of triflumioxazole. Meanwhile, the dicyclooctane-1, 2, 4-oxadiazole derivative provided by the invention also has an outstanding effect in prevention and treatment of the root-knot nematode, and the selectivity of pesticides in the field of prevention and treatment of the root-knot nematode is widened.

The invention discloses a KASP molecular marker linked with a wheat stalk rust temperature-sensitive resistance gene Sr6 and application of the KASP molecular marker, and relates to the technical field of biomolecular detection. The nucleotide sequence of the KASP molecular marker is as shown in SEQ ID NO. 1. The invention also provides application of the KASP molecular marker in identifying the wheat stalk rust temperature-sensitive resistance gene Sr6. According to the present invention, the SNP site closely linked with the wheat stem rust temperature-sensitive resistance gene Sr6 is found through the research, and the KASP molecular marker closely linked with the Sr6 is developed based on the SNP site; the KASP molecular marker can be used for effectively identifying the wheat stalk rust temperature-sensitive resistance gene Sr6, and has the advantages of simple and convenient detection process, rapidness and high flux. The method has important significance on wheat germplasm resource Puccinia graminicola-resistant gene screening, wheat Puccinia graminicola-resistant material creation or wheat Puccinia graminicola-resistant molecule auxiliary breeding.

The invention provides a nucleic acid molecule for coding wheat stripe rust effect protein Pst13248 and application of the nucleic acid molecule, and belongs to the technical field of biological agriculture. The invention provides a nucleic acid molecule for coding wheat stripe rust effect protein Pst13248. According to the invention, Pst13248 of puccinia striiformis is silenced by an RNAi transgenic technology to obtain a Pst13248 silenced wheat material, and a wheat plant with wheat stripe rust resistance is obtained. In an infection experiment of stripe rust CYR32 on wheat, compared with a contrast Fielder, the Pst13248-RNAi wheat plant has the advantage that the Pst13248-RNAi wheat plant shows obvious resistance. Specifically, Pst13248-RNAi is a macroscopic phenotype that a large area of allergic necrosis reaction is generated at a leaf infected part of a wheat plant and uredospores of puccinia striiformis are reduced; the active oxygen area is increased, the cell necrosis area is increased, and strip rust hypha infection is blocked.

The invention belongs to the technical field of gene engineering, and discloses application of a TaPP2C10 gene in wheat stripe rust resistance. The nucleotide sequence of an open reading frame of the TaPP2C10 gene is shown as SEQ ID NO: 1, the gene participates in the resistance of wheat to stripe rust and plays a negative regulation role in the resistance of wheat to stripe rust, and the resistance of wheat to stripe rust is improved by silencing the TaPP2C10 gene. The growth of puccinia striiformis in a TaPP2C10 gene silent plant is obviously inhibited, the fungal biomass is obviously lower than that of a control plant, and the hypha length and the total infection area of puccinia striiformis in the silent plant are also obviously smaller than that of the control plant. A germplasm resource is provided for green prevention and control of the wheat stripe rust, and a new idea is provided for cultivation of rust-resistant wheat varieties.

The invention relates to the field of biotechnology engineering, in particular to an Asian soybean rust resistance gene and application thereof. The resistance gene is RppGM12H3 or RppGM12H4 and has the characteristics of low cytotoxicity and the like, and the obtained transgenic material has an efficient and broad-spectrum control effect on different puccinia puccinia strains.

The invention discloses a 2-cyano-3-amino-3-substituted phenyl acrylate compound and application of the 2-cyano-3-amino-3-substituted phenyl acrylate compound in prevention and treatment of fungal diseases of crops, and belongs to the field of pesticides. The structural formula of the 2-cyano-3-amino-3-substituted phenyl acrylate compound is shown as a formula (I), and the compound has good broad-spectrum bactericidal activity; the compound can be used for preventing and treating plant fungal diseases, especially plant fungal diseases caused by fungi such as pyrethrospora, chlorosclerotium, fusarium, acrocyst, colletotrichum, cercospora, verticillium, black beetle, alternaria, bipolaris, rhizoctonia, bipolaris, botrytis, puccinia, coracosporium and corynespora, and can be used for preventing and treating plant fungal diseases caused by fungi such as pyrethrospora, chlorosclerotium, fusarium, acrocyst, colletotrichum, colletotrichum, colletotrichum, verticillium, black beetle, alternaria. Therefore, the compound can be used for preparing bactericides in the fields of agriculture, horticulture, industry and the like, and has the advantages of broad spectrum, high efficiency, low toxicity, environment friendliness and the like.

This invention belongs to the field of plant genetic engineering technology, specifically relating to stripe rust fungus effector protein genes. Ps13167 The invention relates to the application of its encoded proteins in regulating plant resistance to stripe rust. This invention utilizes an Agrobacterium-mediated wheat genetic transformation system to transfer stripe rust effector protein genes... Ps13167 The silent and full-length fragments of the gene were transformed into wheat embryos, resulting in gene-silenced plants and overexpressing plants, respectively. Phenotypic identification revealed that the silent plants exhibited significant resistance to wheat stripe rust, while the overexpressing plants showed weakened resistance to stripe rust. Furthermore, this invention demonstrates the effectiveness of the stripe rust effector protein gene... Ps13167 Its important role in wheat infection by stripe rust and the ability to utilize stripe rust effector protein genes Ps13167 Improve the disease resistance of wheat, so as to breed genetic material resistant to rust.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a stripe rust effect protein gene Ps13167 or an encoding protein thereof in regulating and controlling the stripe rust resistance of plants. A silent segment and a full-length segment of a stripe rust effect protein gene Ps13167 are transformed into a wheat immature embryo by utilizing an agrobacterium-mediated wheat genetic transformation system, a gene silent plant and an overexpression plant are respectively obtained, and phenotypic identification finds that the silent plant shows remarkable resistance to wheat stripe rust; the over-expressed plant shows weakened resistance to stripe rust. Furthermore, the invention proves that the puccinia striiformis effect protein gene Ps13167 plays an important role in wheat infected by puccinia striiformis and can be used for improving the disease resistance of wheat, so that the puccinia striiformis effect protein gene Ps13167 is used for culturing a rust-resistant genetic material.