The invention relates to a sterilization composition in the field of pesticide preparation. The active ingredients of the sterilization composition include tetraconazole and simeconazole which are mixed, wherein the weight percent ratio of the two active ingredients is (80:1)-(1:80), preferably (50:1)-(1:50); the total content of the active ingredients in the sterilization composition in percentage by weight is 1-90%, preferably 5-50%; and other components in the sterilization composition are allowed and acceptable auxiliary components. The sterilization composition containing tetraconazole and simeconazole can be prepared into wettable powder, suspending agent, missible oil, soluble liquid, emulsion in water and water dispersible granules; and the sterilization composition provided by the invention is mainly used for preventing and controlling diseases caused by erysiphe, puccinia, rhynchosporium, pyrenophora and septoria.