273 results about "Root-knot nematode" patented technology

The invention relates to application of a nitrostyrolene compound in preventing, controlling or resisting plant pathogenic nematodes and a preparation of the nitrostyrolene compound, and relates to the field of pesticides. The nitrostyrene compound is selected from the group consisting of beta-nitrostyrene, trans-nitrostyrene, trans-4-fluoro-beta-nitrostyrene, trans-4-bromo-beta-nitrostyrene, 4-methoxy-beta-nitrostyrene, trans-4-(2-nitrovinyl) cyanobenzene, 1-(3-fluorophenyl)-2-nitroethylene, (E)-1-fluoro-2-(2-nitrovinyl) and one or more of benzene. The nitrostyrene compound shows excellent insecticidal activity on plant root-knot nematodes and pine wood nematodes, has the advantages of simple structure, easiness in synthesis, good control effect and the like, and has a good application prospect in the aspect of controlling root-knot nematode diseases and pine wood nematode diseases. The compound can be applied to development of novel pesticide preparations for killing root-knot nematodes and pine wood nematodes.

The invention provides a spiropolyspora roseus strain for preventing and treating root knot nematode disease, and the preservation number of the spiropolyspora roseus strain is CGMCC No.41822. The invention also provides a microbial inoculum containing the spiropolyspora pink strain, and the microbial inoculum is prepared by the following steps: inoculating a culture medium with the spiropolyspora pink HLF-14-11, and culturing until spores are produced; spores are eluted to prepare a spore suspension, or a bacterial block is cut to serve as a liquid fermentation inoculant; inoculating the inoculant into a liquid culture medium, and carrying out shake culture; and taking the fermentation liquor to prepare liquid, powder and granules. The spiropolyspora pink strain provided by the invention is separated from root-knot nematode oocysts of diseased tomatoes, can parasitize eggs of root-knot nematodes and inhibit incubation of the eggs, fermentation filtrate of the spiropolyspora pink strain has a relatively strong poisoning effect on second-stage larvae of the root-knot nematodes, and the fatality rate of the second-stage larvae reaches 89.1%. Meanwhile, the strain can produce auxin substances such as indoleacetic acid and the like, and plant growth and root development are promoted.

The invention belongs to the field of microorganisms, and particularly discloses a Pseudomonas klackmussii strain TYQ5 and application thereof, the Pseudomonas klackmussii strain TYQ5 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.27214, and the Pseudomonas klackmussii strain TYQ5 is preserved in the China General Microbiological Culture Collection Center (CGMCC). The applicant finds that the Pseudomonas kirschner can secrete auxin, can promote the growth of cucumber seedlings, also has the capabilities of interfering tropism migration of nematodes and killing nematodes, has the effect of remarkably relieving stress of root-knot nematodes, also finds that the Pseudomonas kirschner has the capability of inhibiting normal growth of fusarium oxysporum, and also finds that the Pseudomonas kirschner has the capability of inhibiting normal growth of fusarium oxysporum. Furthermore, the pseudomonas kirschner has the capability of degrading vegetable straws and the capability of solubilizing phosphorus, so that the applicant considers that the pseudomonas kirschner has a remarkable application prospect in the fields of agriculture and environmental protection.

The invention belongs to the field of microorganisms, and particularly discloses a microbacterium paraoxidans strain and application thereof, the microbacterium paraoxidans strain TYQ7 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.27216, and the preservation number is CGMCC No.27216. The microbacterium paraoxidans strain TYQ7 has the advantages that the microbacterium paraoxidans strain TYQ7 is preserved in the China General Microbiological Culture Collection Center (CGMCC); the applicant finds that the microbacterium paraoxidans can secrete auxin, can promote growth of cucumber seedlings, also has the capabilities of interfering tropism migration of nematodes and killing nematodes, has the effect of remarkably relieving stress of root-knot nematodes, also finds that the microbacterium paraoxidans has the capability of inhibiting normal growth of fusarium oxysporum, and has the effect of remarkably relieving stress of root-knot nematodes. Furthermore, the microbacterium paraoxidans have the capability of degrading vegetable straws and the capability of dissolving phosphorus, so that the applicant considers that the microbacterium paraoxidans have a remarkable application prospect in the fields of agriculture and environmental protection.

The invention relates to the technical field of microbial control, in particular to Korean pseudomonas, fermentation liquor, a microbial agent and application. The Korean pseudomonas is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.31567. The Korean pseudomonas Sneb2094 can be used for effectively preventing and treating the root knot nematode disease and promoting the plant growth.

The invention belongs to the technical field of biological prevention and control of plant pests, and relates to application of streptomyces sdu1201 and / or secondary metabolites in prevention and control of plant pests. Research finds that the streptomyces sp. Sdu1201 and the secondary metabolite thereof have the effect of preventing and treating tetranychus urticae and root-knot nematode.

The invention relates to the technical field of fertilizers and pesticides for agricultural production, and discloses a liquid pesticide fertilizer for preventing and treating root-knot nematodes in a water-fertilizer integrated system and application thereof, and the liquid pesticide fertilizer is prepared from the following components: a plant source nematicide, a biocontrol inoculant, a macroelement and medium trace element fertilizer, a boron cross-linking agent, a modified polycarboxylic acid dispersing agent, a microbial protective agent and the like. The preparation method comprises the steps of biological phase pretreatment, matrix phase construction, oil phase micro-emulsification, multiphase fusion and dynamic crosslinking. Through trehalose gradient permeation pretreatment, the survival rate of biocontrol bacteria in a high-salt matrix is increased; a boric acid ester bond dynamic cross-linked network is constructed by using a variable pH adjusting process, so that the system is endowed with shear thinning characteristics, and the contradiction between suspension stability and drip irrigation fluidity is solved; and the chelating dispersion effect of the modified polycarboxylic acid dispersing agent is matched, so that the generation of precipitates is effectively inhibited, and stable coexistence and synergistic interaction of the pesticide, the fertilizer and the bacteria are realized.

The invention provides a bromo-pyrrole nitrile-beta-aminobutyric acid derivative as well as a preparation method and application thereof, and belongs to the technical field of preparation of pesticide compounds. The bromopyrrole nitrile-beta-aminobutyric acid derivative disclosed by the invention is a derivative of 3-(2-(3-bromo-5-(4-chlorphenyl)-4-cyano-2-(trifluoromethyl)-1H-pyrrole-1-yl) acetamido)-2-methyl propionic acid and ester of 3-(2-(3-bromo-5-(4-chlorphenyl)-4-cyano-2-(trifluoromethyl)-1H-pyrrole-1-yl) acetamido)-2-methyl propionic According to the invention, bromo-pyrrolo-nitrile is used as a parent structure and is subjected to active substructure splicing with different substituted beta-aminobutyric acid derivatives to synthesize a series of novel compounds, the chemical structure is novel, and the compounds can be used for preventing and treating plant parasitic nematodes such as root-knot nematodes and cyst nematodes; a biological assay result shows that the prevention effect of bromo-pyrrolecarbonitrile-beta-aminobutyric acid on tomato root-knot nematodes is obviously superior to that of a commercial medicament fluopyram and is equivalent to that of a novel nematicide trifluoropyridylamine, and the bromo-pyrrolecarbonitrile-beta-aminobutyric acid has a relatively high commercial prospect.

The invention relates to the technical field of organic chemistry, in particular to application of pyrazine compounds in the aspect of killing nematodes. The pyrazine compound has good nematicidal activity on pine wood nematodes, root-knot nematodes and aphelenchoides besseyi, and part of the pyrazine compound also has good activity on root knot nematode diseases caused by the root-knot nematodes, so that the pyrazine compound has good application prospects in the aspect of root knot nematode disease resistance.

The invention discloses nematode-killing streptomyces JXSY860 and application thereof, and belongs to the technical field of microbial pesticides. According to the invention, a new strain of Streptomyces turiscabs JXSY860 is found, the new strain of Streptomyces turiscabs JXSY860 has been preserved in Guangdong Microbial Culture Collection Center on August 8, 2024, and the preservation number is GDMCC No: 64998. The invention also discloses a preparation method of the Streptomyces turiscabs JXSY860. Through verification, the JXSY860 strain provided by the invention has stronger lethal activity on second-stage larvae of root-knot nematode, has stronger inhibition effect on incubation of root-knot nematode eggs, has important application value on prevention and control of root-knot nematode diseases of crops, and can be widely applied to preparation of biological agents for preventing and controlling the root-knot nematode diseases.

The invention provides a preparation method of a root knot nematode disease plant source nematicide, and belongs to the technical field of computational chemistry. The preparation method comprises the following steps: firstly, collecting tobacco plant materials in a root knot nematode disease high-incidence area, drying and crushing; determining optimal extraction parameters through a plant extraction parameter optimization function, and carrying out reflux extraction by taking ethanol as a solvent; filtering and concentrating an extracting solution, and adding an organic silicon surfactant and xanthan gum to enhance the stability; adjusting the pH value and adding a preservative; a high-pressure homogenizer is used for treatment, so that the dispersion uniformity is improved; component analysis and activity evaluation are carried out through a plant nematicidal activity prediction model, and the model is based on a convolutional neural network and a Transform architecture, and accurate prediction is realized through an adaptive attention mechanism; and filling the preparation and storing in a dark place. The whole preparation process realizes standardized control of components, and solves the technical problem of large difference between batches of traditional plant source nematocides.

The invention relates to the technical field of prevention and control of microorganisms and nematodes, in particular to arthrobacter pastoris and application thereof. The arthrobacter pastoris disclosed by the invention is classified and named as Arthrobacter pascens, the strain number of the arthrobacter pastoris is YCH-T2, the arthrobacter pastoris is preserved in the China General Microbiological Culture Collection Center on March 19, 2024, and the preservation number of the arthrobacter pastoris is CGMCC No.30072. The strain has strong lethal activity on second-stage larvae of the root-knot nematode, has strong inhibition effect on incubation of eggs of the root-knot nematode, and has important application value in prevention and treatment of root-knot nematode diseases of crops.

The invention relates to the technical field of microbial pesticides and microbial fertilizers, in particular to actinomycetes antagonizing various plant pathogens and application of the actinomycetes antagonizing various plant pathogens, and provides a YC3041 strain with the taxonomic name of Streptomyces pratensis and the preservation number of GDMCC No: 64828. The technical scheme provided by the invention has the characteristics of low use cost, good control effect, no residue and safety to human, livestock and environment, and the actinomycetes antagonizing various plant pathogens and the application of the actinomycetes antagonizing various plant pathogens have broad application prospects. The biological pesticide prepared from the YC3041 strain has the control effect of 70% or above on tobacco root knot nematode disease, tobacco bacterial wilt, tobacco black shank and compound diseases caused by the tobacco root knot nematode disease, the tobacco bacterial wilt and the tobacco black shank.

The invention relates to an insecticide composition for preventing and treating root-knot nematodes and application thereof, and belongs to the field of agricultural nematode killing compositions. The effective components of the nematicidal composition provided by the invention are compounded by two spirotetramat and hymexazol, and the weight ratio of two spirotetramat to hymexazol is (1: 80)-(80: 1), preferably (1: 20)-(20: 1). A preparation prepared from the compound has very high activity on root-knot nematodes; the composition is not simple superposition of the activities of the two components, but has a remarkable synergistic effect; the field dosage of the effective components is reduced, the production and use cost is reduced, and pesticide residues and environmental pollution are reduced; the composition is composed of effective components with different action mechanisms, and the action mechanisms complement each other, so that the composition is beneficial to overcoming and delaying the generation of pest resistance to drugs, and is safe to crops.

The invention discloses a group of polymorphic molecular markers of tobacco southern root knot nematode disease resistance site Rk1 and application thereof. The invention relates to a group of polymorphic molecular markers of tobacco southern root knot nematode disease resistance sites Rk1, which are positioned in a 1-65Mb region of Chr17 # chromosome of a K326 genome. The primer for detecting the polymorphic molecular marker is a primer for detecting the polymorphic molecular marker, and the nucleotide sequence of the primer is any one of seven groups of primers as shown in SEQ ID No.1-14. The polymorphic molecular marker is applied to identification of tobacco southern root knot nematode disease resistant materials or varieties, or is applied to improvement of tobacco southern root knot nematode disease resistance. The polymorphic molecular marker is distributed in an exogenous translocation chromosome segment, has good polymorphism in a natural population, and can accurately detect the meloidogyne incognita resistant Rk1 gene of tobacco.

The invention relates to paenibacillus strongly ZKSW001 as well as a fungicide and application thereof, and belongs to the technical field of microorganisms. The paenibacillus pumilus ZKSW001 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.36076, the preservation date is September 25, 2025, and the address of the preservation institution is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention also provides application of the paenibacillus strongly ZKSW001 in inhibition of harmful bacteria in soil, prevention and treatment of root-knot nematode and promotion of plant growth. The paenibacillus strongly ZKSW001 disclosed by the invention not only can promote the growth of crops and realize weight loss and efficiency improvement, but also can control the occurrence of soil-borne fungal and bacterial diseases, and can inhibit the growth of root-knot nematode. Chemical pesticide application can be reduced, pesticide residues are reduced, and sustainable development of agricultural economy is promoted.

The invention provides an endophyte Gliocladium roseum NG-12, which is named as Gliocladium roseum NG-12, and the preservation number of the endophyte Gliocladium roseum NG-12 is CGMCC (China General Microbiological Culture Collection Center) No. 41801. The endophyte Gliocladium roseum NG-12 provided by the invention has good root-knot nematode killing activity and antibacterial activity, has a good antagonistic effect on various plant pathogens such as fusarium oxysporum, colletotrichum gloeosporioides and colletotrichum gloeosporioides, is a potential biological preparation for preventing and treating root-knot nematode diseases, fusarium wilt, anthracnose and the like, and also has the effects of dissolving potassium, fixing nitrogen, dissolving phosphorus, preventing and treating the root-knot nematode diseases and the like. The capability of producing siderophore, protease and cellulase is realized. The gliocladium roseum provided by the invention has a wide development space and a good development and application prospect.

The invention discloses paecilomyces lilacinus M-2 for degrading fosthiazate in greenhouse vegetable soil and a preparation method of the paecilomyces lilacinus M-2. The bacterial strain is paecilomyces lilacinus M-2, and the preservation number of the bacterial strain is GDMCC No: 65294. The invention further discloses a preparation method of the paecilomyces lilacinus strain. The paecilomyces lilacinus M-2 provided by the invention is domesticated and prepared into the fosthiazate degrading microbial inoculum, and experiments prove that the degrading microbial inoculum has a rapid degradation effect on pesticides in greenhouse vegetable soil polluted by fosthiazate, and meanwhile, the control effect of fosthiazate on root-knot nematode is not influenced; meanwhile, enhancement of drug resistance of pathogenic bacteria caused by the action of chemical pesticides is effectively reduced, and the balance of a soil micro-ecosystem is protected.

The invention provides a cape jasmine root-knot nematode prevention and control method, and relates to the technical field of plant protection, the cape jasmine root-knot nematode prevention and control method comprises the four steps of preparation of a compound material, compound synergism with an organic bacterial fertilizer, field application and management after application, and according to the cape jasmine root-knot nematode prevention and control method, ricin in castor seed powder slurry can rapidly paralyze and kill root-knot nematode through direct contact; beneficial microorganisms and metabolites thereof in the fermented gardenia oil meal can induce plant root systems to generate resistance, the tolerance of plants to root-knot nematodes is improved, finally, microorganisms in the organic bacterial fertilizer can inhibit incubation and development of root-knot nematode eggs, the breeding and survival rate of nematodes is further reduced, and the yield of the nematodes is increased. Through the synergistic effect of the castor seed powder slurry, the fermented gardenia oil meal and the organic bacterial fertilizer, a contact killing-resistance induction-ovum parasitism triple prevention and control mechanism is formed, and the prevention and control effect can be well improved.

The present invention belongs to the technical field of pesticides, and specifically relates to a pesticide composition for preventing and treating root-knot nematodes of Momordica grosvenori. A pesticide composition for preventing and treating root-knot nematodes of Momordica grosvenori, wherein the active ingredients are compounded with ivermectin and chlorfenapyr, iprodione or prothioconazole. When the active ingredients of the nematicidal composition of the present invention are compounded, the co-toxicity coefficient to the second-instar larvae of the southern root-knot nematode is greater than 120, showing a synergistic effect, which can improve the control effect thereof, and further provide support for the research and development of highly effective agents for preventing and treating root-knot nematodes.

The application provides a Bacillus thuringiensis strain, and the preservation number of the Bacillus thuringiensis strain is GDMCC No. 67284. The lethal rate of the Bacillus thuringiensis strain provided by the application to second instar larvae of root-knot nematode reaches 98%, the control effect in a pot experiment reaches 60.07%, and the control effects on root rot and wilt are 66.79% and 63.23% respectively. The Bacillus thuringiensis strain is a bacillus isolated from rhizosphere soil, is safe to crops, and has control effects on root rot, wilt and root-knot nematode, and has the safety characteristics in agricultural application.

The present invention discloses a microbial inoculant for preventing both root-knot nematode disease and clubroot disease and a preparation method thereof, relating to the technical field of fertilizers, and solving the technical problems in the prior art that the field symptoms of root-knot nematode disease and clubroot disease are similar and difficult to quickly distinguish and accurately select agents, and that root-knot nematode disease and clubroot disease are likely to occur simultaneously and difficult to control in a timely manner. The preparation method of the microbial inoculant for preventing both root-knot nematode disease and clubroot disease of the present invention comprises the following steps: preparing methylotrophic bacillus powder and bacillus subtilis powder; preparing paecilomyces lilacinus powder; and compounding the prepared methylotrophic bacillus powder, paecilomyces lilacinus powder and bacillus subtilis powder in proportion and mixing them evenly to obtain the microbial inoculant for preventing both root-knot nematode disease and clubroot disease. The microbial inoculant for preventing both root-knot nematode disease and clubroot disease prepared by the present invention can conduct unified prevention and control of the two diseases, avoiding the golden period of early prevention and control being delayed due to incorrect agent selection in agricultural production.

The invention relates to the technical field of plant growth-promoting bacteria and soil-borne disease biological control, in particular to a bacillus velezensis 1708Y11-01 strain and application thereof. According to the bacillus velezensis 1708Y11-01 and the application thereof, the bacillus velezensis 1708Y11-01 is separated from plant rhizosphere disease-inhibiting soil, and the bacillus velezensis 1708Y11-01 has a very high control effect on fusarium wilt and root knot nematode disease, and can be used for improving the resistance of a plant system, improving the leaf chlorosis of crops caused by disease infection and promoting the growth of the crops. The bacillus velezensis 1708Y11-01 strain provided by the invention is pollution-free, residue-free and eco-friendly in the application process, and is a biocontrol strain with a good application prospect in the field of disease prevention and control.

The invention belongs to the technical field of biological control, and particularly relates to a method for extracting a chemical substance with obvious activity on root-knot nematode. According to the method for extracting the chemical substance with the obvious activity on the root-knot nematode, the hedyotis diffusa sample is pretreated, impurities on the surface of the sample can be removed easily, and the purity of the subsequent active substance is improved; a sample is soaked in an ethanol solution with the volume concentration of 95%, effective active substances in the sample can be separated out easily, and effective chemical substances in the sample are promoted to be completely released through two times of ultrasonic extraction. Therefore, the chemical substance with obvious activity on the root-knot nematodes can be used for preventing and treating the root-knot nematodes or preparing biological products for preventing and treating the root-knot nematodes, not only is the death rate of the root-knot nematodes improved, but also green and health of plants are guaranteed, and the economic value of the plants is improved.

The invention relates to application of a dazomet liquid preparation in soil treatment in the field of pesticides. The liquid preparation can be used for preventing and treating true and bacterial diseases, nematodes and weeds of crops, and has a remarkable synergistic effect on soil-borne bacteria, fungal diseases, root-knot nematodes and field weeds. The pesticide application mode can be selected from one or more of drip irrigation, flushing application, mist spraying and soil layer treatment, and the pesticide can be directly used or used after being diluted for multiple times during pesticide application. Besides, the core advantage of the application is that the application limitation of the traditional dazomet preparation is broken through, and the dazomet preparation is suitable for wide regions, especially suitable for low-temperature regions and environments with the soil temperature being lower than 10 DEG C, and is especially preferably applied to rice seedling raising fields in low-temperature regions; multiple operation modes such as soil mixing, pulping and rice hull mixing can be adopted in a low-temperature scene, the operation is convenient and fast, the actual field requirements are met, and the environmental adaptability and the application flexibility of the dazomet preparation are remarkably improved.

The invention provides a multifunctional paenibacillus terrae capable of degrading organophosphorus pesticides, resisting diseases and promoting growth, the strain is paenibacillus terrae and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.30973. The invention further provides a preparation method of the paenibacillus terrae. The strain can degrade organophosphorus pesticides: profenofos, malathion, diazinon, phoxim and fenthion, wherein the degradation effect on the diazinon is the best; the growth and development of meloidogyne incognita can be inhibited, and tomato rhizobium caused by the meloidogyne incognita Fusarium oxysporum can be antagonized, and tomato fusarium wilt caused by the fusarium oxysporum can be prevented and treated; the fertilizer can increase the supply of rapidly available phosphorus in soil and promote the growth of leaf vegetables.

The invention relates to the technical field of pest repellents, and discloses an L-tryptophan amide salt tobacco root-knot nematode inhibitor. The structural formula of the active ingredient of the L-tryptophan amide salt tobacco root-knot nematode inhibitor provided by the invention is # imgabs0 #. The L-tryptophan amide salt tobacco root-knot nematode inhibitor provided by the invention has a good inhibition effect on tobacco root-knot nematode, and can effectively block a parasitic channel of the root-knot nematode and restore normal growth of plants; the L-tryptophan amide salt type tobacco root-knot nematode inhibitor provided by the invention has relatively high biological safety.

The invention discloses a dark-color isolated endophytic fungus strain and application thereof, the strain is a dark-color isolated endophytic fungus strain YMF1.068, the preservation name is dark-color isolated endophytic fungus (Paraphoma vinacea), the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the address of the preservation unit is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.689. The preservation date is May 20, 2024; the preservation number is CGMCC (China General Microbiological Culture Collection Center) The strain can effectively prevent and treat root knot nematode disease, has high lethal activity, enhances plant resistance, is safe to human and livestock, and has no residue. The microbial agent prepared by utilizing the strain shows the control effect of 68% or above when being applied to tomatoes, cucumbers and tobaccos, the effect of the microbial agent is equivalent to that of a chemical pesticide, and the potential and market prospect of the microbial agent as a biological pesticide are shown.

The invention provides a micro-capsule suspending agent containing fluopyram as well as a preparation method and application of the micro-capsule suspending agent, and belongs to the technical field of pesticide preparations. The micro-capsule suspending agent containing fluopyram is obtained by adopting a suspension polymerization method, and the obtained micro-capsule suspending agent is high in encapsulation efficiency and excellent in dispersity, has good ultraviolet stability and heat storage stability, and has a good prevention and removal effect and a long lasting period when being used for preventing and treating root nematodes. After root-irrigation pesticide application, the micro-capsule suspending agent containing fluopyram provided by the invention can be slowly released in soil and roots, has a good prevention effect on tomato and cucumber root-knot nematode, and has the advantages of long lasting period, multiple prevention and control crops, wide bactericidal spectrum and excellent uptake conductivity.

The invention provides a bacillus velezensis QT1 and an application of the bacillus velezensis QT1. The classification name of the bacillus velezensis is bacillus velezensis QT1, and the bacillus velezensis is preserved in the China Center for Type Culture Collection on November 12, 2025, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20252531. The strain shows remarkable biological control efficiency, realizes effective control on various plant pathogens, and can prevent and treat tomato bacterial wilt and tea tree anthracnose. In addition, the bacillus velezensis QT1 can also effectively kill root-knot nematodes (especially meloidogyne incognita). When the strain is applied to plant cultivation, the growth of various pathogens can be remarkably inhibited, the health and yield of crops are improved, the strain is environment-friendly, the use of chemical pesticides is reduced, soil pollution is avoided, and the development requirement of sustainable agriculture is met.