168 results about "Root-knot nematode" patented technology

The invention relates to the technical field of fertilizers and pesticides for agricultural production, and discloses a liquid pesticide fertilizer for preventing and treating root-knot nematodes in a water-fertilizer integrated system and application thereof, and the liquid pesticide fertilizer is prepared from the following components: a plant source nematicide, a biocontrol inoculant, a macroelement and medium trace element fertilizer, a boron cross-linking agent, a modified polycarboxylic acid dispersing agent, a microbial protective agent and the like. The preparation method comprises the steps of biological phase pretreatment, matrix phase construction, oil phase micro-emulsification, multiphase fusion and dynamic crosslinking. Through trehalose gradient permeation pretreatment, the survival rate of biocontrol bacteria in a high-salt matrix is increased; a boric acid ester bond dynamic cross-linked network is constructed by using a variable pH adjusting process, so that the system is endowed with shear thinning characteristics, and the contradiction between suspension stability and drip irrigation fluidity is solved; and the chelating dispersion effect of the modified polycarboxylic acid dispersing agent is matched, so that the generation of precipitates is effectively inhibited, and stable coexistence and synergistic interaction of the pesticide, the fertilizer and the bacteria are realized.

The invention discloses nematode-killing streptomyces JXSY860 and application thereof, and belongs to the technical field of microbial pesticides. According to the invention, a new strain of Streptomyces turiscabs JXSY860 is found, the new strain of Streptomyces turiscabs JXSY860 has been preserved in Guangdong Microbial Culture Collection Center on August 8, 2024, and the preservation number is GDMCC No: 64998. The invention also discloses a preparation method of the Streptomyces turiscabs JXSY860. Through verification, the JXSY860 strain provided by the invention has stronger lethal activity on second-stage larvae of root-knot nematode, has stronger inhibition effect on incubation of root-knot nematode eggs, has important application value on prevention and control of root-knot nematode diseases of crops, and can be widely applied to preparation of biological agents for preventing and controlling the root-knot nematode diseases.

The invention discloses a group of polymorphic molecular markers of tobacco southern root knot nematode disease resistance site Rk1 and application thereof. The invention relates to a group of polymorphic molecular markers of tobacco southern root knot nematode disease resistance sites Rk1, which are positioned in a 1-65Mb region of Chr17 # chromosome of a K326 genome. The primer for detecting the polymorphic molecular marker is a primer for detecting the polymorphic molecular marker, and the nucleotide sequence of the primer is any one of seven groups of primers as shown in SEQ ID No.1-14. The polymorphic molecular marker is applied to identification of tobacco southern root knot nematode disease resistant materials or varieties, or is applied to improvement of tobacco southern root knot nematode disease resistance. The polymorphic molecular marker is distributed in an exogenous translocation chromosome segment, has good polymorphism in a natural population, and can accurately detect the meloidogyne incognita resistant Rk1 gene of tobacco.

The invention relates to paenibacillus strongly ZKSW001 as well as a fungicide and application thereof, and belongs to the technical field of microorganisms. The paenibacillus pumilus ZKSW001 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.36076, the preservation date is September 25, 2025, and the address of the preservation institution is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention also provides application of the paenibacillus strongly ZKSW001 in inhibition of harmful bacteria in soil, prevention and treatment of root-knot nematode and promotion of plant growth. The paenibacillus strongly ZKSW001 disclosed by the invention not only can promote the growth of crops and realize weight loss and efficiency improvement, but also can control the occurrence of soil-borne fungal and bacterial diseases, and can inhibit the growth of root-knot nematode. Chemical pesticide application can be reduced, pesticide residues are reduced, and sustainable development of agricultural economy is promoted.

The invention provides a cape jasmine root-knot nematode prevention and control method, and relates to the technical field of plant protection, the cape jasmine root-knot nematode prevention and control method comprises the four steps of preparation of a compound material, compound synergism with an organic bacterial fertilizer, field application and management after application, and according to the cape jasmine root-knot nematode prevention and control method, ricin in castor seed powder slurry can rapidly paralyze and kill root-knot nematode through direct contact; beneficial microorganisms and metabolites thereof in the fermented gardenia oil meal can induce plant root systems to generate resistance, the tolerance of plants to root-knot nematodes is improved, finally, microorganisms in the organic bacterial fertilizer can inhibit incubation and development of root-knot nematode eggs, the breeding and survival rate of nematodes is further reduced, and the yield of the nematodes is increased. Through the synergistic effect of the castor seed powder slurry, the fermented gardenia oil meal and the organic bacterial fertilizer, a contact killing-resistance induction-ovum parasitism triple prevention and control mechanism is formed, and the prevention and control effect can be well improved.

The application provides a Bacillus thuringiensis strain, and the preservation number of the Bacillus thuringiensis strain is GDMCC No. 67284. The lethal rate of the Bacillus thuringiensis strain provided by the application to second instar larvae of root-knot nematode reaches 98%, the control effect in a pot experiment reaches 60.07%, and the control effects on root rot and wilt are 66.79% and 63.23% respectively. The Bacillus thuringiensis strain is a bacillus isolated from rhizosphere soil, is safe to crops, and has control effects on root rot, wilt and root-knot nematode, and has the safety characteristics in agricultural application.

The invention relates to the technical field of plant growth-promoting bacteria and soil-borne disease biological control, in particular to a bacillus velezensis 1708Y11-01 strain and application thereof. According to the bacillus velezensis 1708Y11-01 and the application thereof, the bacillus velezensis 1708Y11-01 is separated from plant rhizosphere disease-inhibiting soil, and the bacillus velezensis 1708Y11-01 has a very high control effect on fusarium wilt and root knot nematode disease, and can be used for improving the resistance of a plant system, improving the leaf chlorosis of crops caused by disease infection and promoting the growth of the crops. The bacillus velezensis 1708Y11-01 strain provided by the invention is pollution-free, residue-free and eco-friendly in the application process, and is a biocontrol strain with a good application prospect in the field of disease prevention and control.

The invention relates to application of a dazomet liquid preparation in soil treatment in the field of pesticides. The liquid preparation can be used for preventing and treating true and bacterial diseases, nematodes and weeds of crops, and has a remarkable synergistic effect on soil-borne bacteria, fungal diseases, root-knot nematodes and field weeds. The pesticide application mode can be selected from one or more of drip irrigation, flushing application, mist spraying and soil layer treatment, and the pesticide can be directly used or used after being diluted for multiple times during pesticide application. Besides, the core advantage of the application is that the application limitation of the traditional dazomet preparation is broken through, and the dazomet preparation is suitable for wide regions, especially suitable for low-temperature regions and environments with the soil temperature being lower than 10 DEG C, and is especially preferably applied to rice seedling raising fields in low-temperature regions; multiple operation modes such as soil mixing, pulping and rice hull mixing can be adopted in a low-temperature scene, the operation is convenient and fast, the actual field requirements are met, and the environmental adaptability and the application flexibility of the dazomet preparation are remarkably improved.

The invention provides a micro-capsule suspending agent containing fluopyram as well as a preparation method and application of the micro-capsule suspending agent, and belongs to the technical field of pesticide preparations. The micro-capsule suspending agent containing fluopyram is obtained by adopting a suspension polymerization method, and the obtained micro-capsule suspending agent is high in encapsulation efficiency and excellent in dispersity, has good ultraviolet stability and heat storage stability, and has a good prevention and removal effect and a long lasting period when being used for preventing and treating root nematodes. After root-irrigation pesticide application, the micro-capsule suspending agent containing fluopyram provided by the invention can be slowly released in soil and roots, has a good prevention effect on tomato and cucumber root-knot nematode, and has the advantages of long lasting period, multiple prevention and control crops, wide bactericidal spectrum and excellent uptake conductivity.

The invention provides a bacillus velezensis QT1 and an application of the bacillus velezensis QT1. The classification name of the bacillus velezensis is bacillus velezensis QT1, and the bacillus velezensis is preserved in the China Center for Type Culture Collection on November 12, 2025, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20252531. The strain shows remarkable biological control efficiency, realizes effective control on various plant pathogens, and can prevent and treat tomato bacterial wilt and tea tree anthracnose. In addition, the bacillus velezensis QT1 can also effectively kill root-knot nematodes (especially meloidogyne incognita). When the strain is applied to plant cultivation, the growth of various pathogens can be remarkably inhibited, the health and yield of crops are improved, the strain is environment-friendly, the use of chemical pesticides is reduced, soil pollution is avoided, and the development requirement of sustainable agriculture is met.

The application discloses application of 3-(4-fluorophenyl) prop-2-yne carbonitrile in prevention and treatment of plant root knot nematodes and belongs to the technical field of plant root knot nematode prevention and treatment. 50 It is found for the first time that 3-(4-fluorophenyl) prop-2-yne carbonitrile has significant insecticidal activity on southern root knot nematodes, the LC 3.109 μg / mL of 3-(4-fluorophenyl) prop-2-yne carbonitrile on southern root knot nematodes is 72 h, the average mortality reaches 92.5% when the application concentration is 48 μg / mL, and the 3-(4-fluorophenyl) prop-2-yne carbonitrile can be used as a novel and effective drug for plant root knot nematode prevention and treatment.

The present application relates to the field of the prevention of southern root-knot nematode, and discloses a Pseudomonas kribbensis ZJU1, with a preservation number of CCTCC NO: M 20221258.The present application also discloses the use of the Pseudomonas kribbensis ZJU1 in trapping and killing southern root-knot nematode.

The invention provides an alkaloid Waltherione S analogue as well as a synthesis method and application thereof, and relates to the technical field of alkaloid Waltherione compounds. According to the present invention, the structures of the Waltherione A and the Antidesmone are simplified, the pyridine and pyridone alkaloid Waltherione S analogue is designed and synthesized, and the synthesized compound has broad-spectrum bacterial killing activity on banana wilt pathogen, wheat scab pathogen, banana anthracnose pathogen, phytophthora capsici pathogen, botrytis cinerea, rhizoctonia solani, rice blast pathogen, mango stem-end rot pathogen and the like, the compound also has good nematicidal activity on root-knot nematodes, provides a favorable candidate agent for natural, safe and low-toxicity innovative pesticides, and is expected to be applied to agricultural production.

The application discloses application of a benzamide compound in prevention and treatment of plant root-knot nematodes. N‑ (2-ethyl-1,2,5-thiadiazol-4-yl)-2-iodobenzamide. 2H The application discloses a benzamide compound N‑ (2-ethyl-1,2,5-thiadiazol-4-yl)-2-iodobenzamide. 2H The benzamide compound has strong toxic killing effect on various plant root-knot nematodes, and has no insecticidal activity on non-plant pathogenic nematodes, and has the characteristics of high efficiency, low toxicity and low residue. The application provides a new candidate compound for the prevention and treatment of plant root-knot nematodes, enriches the structure type of the root-knot nematode killing benzamide compound, and provides a new thought and way for development of a new root-knot nematode killing pesticide.

The application discloses a seedling stage resistance identification method for salvia miltiorrhiza root knot nematode disease and application thereof. After seedling of salvia miltiorrhiza seeds to the 5-6 leaf stage, 2mL of a 2-stage young nematode (J2) suspension with a concentration of 200 pieces / mL is inoculated, and the root knot condition of salvia miltiorrhiza and the influence of different infection durations on the growth condition of salvia miltiorrhiza are investigated after inoculation. The number of root knots of the to-be-tested variety is investigated on the 9th day after inoculation of the nematodes, if the number of root knots is less than 15, the to-be-tested variety enters the next stage of identification, that is, comprehensive trait investigation is carried out on the to-be-tested variety on the 21st day after inoculation of the nematodes, if there is no significant difference between the root fresh weight, the height of the aboveground part and the leaf fresh weight of the to-be-tested variety and those of the non-inoculation group, the to-be-tested variety is preliminarily determined as a resistant variety. The application provides a rapid, simple and efficient indoor identification method for seedling stage resistance identification of salvia miltiorrhiza root knot nematode disease.

The invention discloses an actinoplanes strain and application thereof. In a first aspect of the disclosure, there is provided an actinoplanes strain, which may be a strain MN311411 having a preservation number of GDMCC No: 64167, or a strain having at least 98.65% identity with 16S rRNA of strain MN311411, or a strain having at least 98.65% identity with the 16S rRNA sequence set forth in SEQ ID NO: 1, and / or a strain having at least 95% average nucleotide identity with the genome of strain MN311411. The embodiment of the invention discloses a novel strain. The strain or a product based on the strain has a good effect of preventing and treating soil-borne plant diseases and insect pests such as root-knot nematodes and the like.

The application of microbial agent and biochar in the prevention and treatment of tomato root-knot nematode disease is disclosed, which relates to the technical field of tomato root-knot nematode prevention and treatment. The microbial agent is Pseudomonas plecoglossicida, the dosage of Pseudomonas plecoglossicida is 2 kg per mu, and the application amount of biochar is 1 t per mu. Pseudomonas plecoglossicida is dripped into water by drip irrigation, and is dripped once every 20 days. Biochar is applied as base fertilizer before tomato planting. After data measurement, arrangement and analysis, it is found that in the greenhouse soil infected with root-knot nematode disease in Hexi region of Gansu province, when the dosage of Pseudomonas plecoglossicida is 2 kg per mu, and the application amount of biochar is 1 t per mu, the tomato grows well under the condition, the quality is better, and the root-knot nematode disease has better control effect.

The application provides a strain of Rhodococcus ZJUHULJT1 and application of the strain in prevention and treatment of paratyphaceae root-knot nematodes, and belongs to the technical field of biological prevention and treatment of root-knot nematodes. Rhodococcus The Rhodococcus (sp.) ZJUHULJT1 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20251182; the Rhodococcus ZJUHULJT1 can significantly inhibit the infection and reproduction of root-knot nematodes, reduce the number of rice root knots, promote the synthesis of defense-related hormones of rice roots, and improve the disease resistance of rice; through the multiple inhibition of paratyphaceae root-knot nematodes, the Rhodococcus ZJUHULJT1 can be applied to the prevention and treatment of rice root-knot nematodes, has good prevention and treatment effect, and provides a new technical means for the pollution-free reduction of paratyphaceae root-knot nematode diseases.

This invention belongs to the field of microbiology, specifically disclosing a strain of *Mcrobacterium paraoxydans* and its applications. The *Mcrobacterium paraoxydans* strain TYQ7 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 27216. The applicant discovered that the aforementioned *Mcrobacterium paraoxydans* can secrete auxin, promoting the growth of cucumber seedlings. It also has the ability to interfere with the directional migration of nematodes and kill nematodes, significantly alleviating root-knot nematode stress. Furthermore, it was found to inhibit the normal growth of *Fusarium oxysporum*, and further exhibits the ability to degrade vegetable straw and solubilize phosphorus. Therefore, the applicant believes that the aforementioned *Mcrobacterium paraoxydans* has significant application prospects in the fields of agriculture and environmental protection.

The invention relates to the crossing field of agricultural biological control and fertilizer technology, and discloses a compound microbial pesticide fertilizer capable of simultaneously controlling root-knot nematode and soil-borne fungal diseases, the pesticide fertilizer has a core-shell structure, and comprises an inner inorganic nutrition core, a middle isolation buffer layer and an outer functional bacterial layer; the raw materials comprise inorganic nutrition core particles, a biological activation organic carrier, composite functional bacterial powder and a modified slow-release binder. Wherein the binder is prepared from oxidized starch and PVA through borax crosslinking, so that the particle strength is enhanced; the carrier is fermented and activated, and synergistic components such as azadirachtin are added. An organic isolation buffer layer is constructed through a layered coating process, osmotic pressure stress of inorganic salt on microorganisms is effectively blocked, and the problem of strain inactivation is solved; the synergistic compounding of paecilomyces lilacinus, pochonia chlamydosporia, trichoderma harzianum and bacillus subtilis is utilized to realize the comprehensive prevention and control of root-knot nematode and soil-borne fungal diseases, and has the functions of nutrient slow release and crop resistance induction.

The application provides a potato stress-resistant high-quality germplasm resource screening and directional breeding improvement method, and belongs to the technical field of crop germplasm resource screening and breeding.The application builds a compound stress garden by standardizing virus-free seedling of potato germplasm, and simultaneously applies four gradient stresses of drought, saline-alkali, night low temperature and continuous cropping of root knot nematode;combined with progressive agronomic phenotype observation of potato seedling stage and tuber bulking stage, inferior single plants are eliminated in advance;nondestructive batch quality detection of tubers is carried out by using near-infrared spectroscopy technology, and the four-dimensional weighted comprehensive scoring system is relied on to complete germplasm grading and screening;finally, the excellent germplasm obtained by screening is divided into groups for seed saving, and through cross-group directional crossing and continuous breeding under stress, a stress-resistant high-quality potato improved line is bred.The new line has multiple stress resistance and excellent commodity and processing quality, and is suitable for large-scale potato germplasm screening and directional breeding popularization and application.

The present disclosure provides compositions and methods for producing plants having resistance to diseases caused by Meloidogyne such as, for example, root-knot nematode. The methods involve expressing in plants a heterologous nucleic acid associated with increased resistance to Meloidogyne. Additionally, compositions and methods useful in producing, identifying and / or selecting plants and plant materials comprising the heterologous nucleic acid associated with increased resistance to Meloidogyne are provided.

The invention discloses application of trichoderma xerophyllum to prevention and treatment of plant root-knot nematodes, and belongs to the technical field of microorganisms, the trichoderma xerophyllum is preserved in Guangdong Microbial Culture Collection Center, the number is GDMCC No: 66977, and the preservation address is No.100, Xianlie Middle Road, Xiaolian District, Guangzhou City, Guangdong Province. The preparation method of the fungicide prepared based on the trichoderma xerophyllum comprises the following steps: transferring the strain into a seed culture medium, and culturing on a shaking table at the temperature of 25-30 DEG C and the speed of 150-200r / min for 24-36 hours to obtain a fermentation seed solution; inoculating the fermentation seed solution into a fermentation culture medium, and culturing for 72-96 hours on a shaking table at the temperature of 25-30 DEG C and the speed of 150-200 r / min to obtain a fermentation solution, namely the target fungicide. The trichoderma xerophyllum has a remarkable lethal effect on root-knot nematodes, the fatality rate of fermentation liquor of the trichoderma xerophyllum on the root-knot nematodes exceeds 90%, the fatality rate of 80% or above can still be kept even if the trichoderma xerophyllum is diluted by 50 times, the trichoderma xerophyllum acts quickly, nematodes can be quickly killed within 12 hours, larvae are effectively prevented from invading plants, and the trichoderma xerophyllum shows a good application prospect in the field of prevention and treatment of the plant root-knot nematodes.

The invention relates to the field of biocontrol of plant diseases. The invention provides a bacillus safensis strain HMQAU23223, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 13, 2025, and the preservation registration number is CGMCC No.36623. The bacillus safensis strain HMQAU23223 has the advantages that the preservation number is CGMCC No.36623; the invention also provides a biocontrol inoculant. The invention also provides a preparation method of the biocontrol inoculant. The invention further provides the application of the bacillus safensis strain HMQAU23223 or the biocontrol microbial inoculum or the biocontrol microbial inoculum prepared by the preparation method. The invention further provides the application of the bacillus safensis strain HMQAU23223 or the biocontrol microbial inoculum prepared by the preparation method. The invention further provides a method for preventing and treating plant diseases. The invention also provides an application of the bacillus safensis strain HMQAU23223 in preparation of a compound for inhibiting phytopathogen or preventing and treating plant pathogenic nematodes. The fermentation filtrate of the bacillus safensis strain HMQAU23223 provided by the invention has the lethal effects of 89% or above on pine wood nematode, meloidogyne incognita, wheat cyst nematode, heterodera filipjevi and ditylenchus destructor, and has better biocontrol potential.

The invention discloses a hydrogel and probiotic compounded microbial inoculum composition and a vegetable planting matrix, a three-dimensional gel carrier is used for embedding a microbial inoculum, the carrier integrates mechanical properties and thallus protection and can maintain flora activity for a long time, bacillus thuringiensis has a low-oxygen survival characteristic after being subjected to Fnr enhanced expression, and the microbial inoculum composition has the advantages that the microbial inoculum composition has a low-oxygen survival characteristic after being subjected to Fnr enhanced expression; the microbial agent composition can survive for a long time in a low-oxygen environment of a carrier embedding environment, and is cooperated with bacillus cereus, azotobacter chroococcum and bacillus megatherium to promote the growth of plants such as vegetables and the like and directionally kill pathogenic microorganisms such as root-knot nematode. Corresponding pests can be killed in a green and targeted manner, the growth of plants is promoted, and the yield is increased.

The application discloses a bacillus licheniformis-derived nematocidal peptide Peptide-1 and application of the nematocidal peptide Peptide-1 in the prevention and control of root-knot nematode diseases, wherein the amino acid sequence of the nematocidal peptide Peptide-1 is shown as SEQ ID NO. 1. The application further provides a medicament for preventing and controlling root-knot nematodes and a prevention and control method thereof. The activity verification result shows that under the condition of 1.00 mmol / L, the corrected mortality of the nematocidal peptide Peptide-1 to the second instar larvae of root-knot nematodes reaches 90.7% after 24 hours, and the nematocidal peptide Peptide-1 has a clear and efficient lethal effect and concentration dependence. The nematocidal peptide Peptide-1 can be used for the green prevention and control of root-knot nematodes and the creation of new germplasm resources of root-knot nematode-resistant crops, meets the requirements of green agricultural development, and can effectively solve the problems of pesticide residues, drug resistance and ecological pollution caused by traditional chemical nematocidal agents.

The application provides application of farnesene in prevention and treatment of southern root-knot nematode, belongs to the technical field of prevention and treatment of root-knot nematode, and the concentration of farnesene in prevention and treatment of southern root-knot nematode is 92-118 ng / h per plant. The application finds that farnesene exposure treatment can effectively inhibit the invasion and reproduction of southern root-knot nematode and significantly reduce the feeding preference of southern root-knot nematode to corn by using farnesene to expose and treat corn plants, and farnesene can improve the resistance of plants by regulating and improving the content of defense hormone jasmonic acid isoleucine complex in corn. In conclusion, the application provides a new idea for green prevention and control of southern root-knot nematode.

The application discloses application of natural unsaturated aliphatic aldehyde in prevention and treatment of plant parasitic nematodes. The natural unsaturated aliphatic aldehyde is trans, trans-2,4-heptadienal and trans-2-heptenal. The plant parasitic nematodes are root-knot nematodes, cyst nematodes and pine wood nematodes. Both the trans, trans-2,4-heptadienal and the trans-2-heptenal can exhibit excellent nematocidal activity through contact killing and fumigation, which provides a basis for development of green and environmentally-friendly nematocidal agents, and has a good application prospect for replacing traditional chemical nematocidal agents.

The application provides a strain of protozoan nephrospora NJAU-HN1 and an application thereof, and belongs to the technical field of agricultural microorganisms. The nephrospora NJAU-HN1 has a preservation number of CCTCC NO: C2025172. The nephrospora NJAU-HN1 can directly and effectively inhibit the survival of root-knot nematodes. Application of the nephrospora NJAU-HN1 or a culture thereof can significantly promote the growth of melon plants, increase the fresh weight, dry weight and root length of the plants. The nephrospora NJAU-HN1 provided by the application provides valuable microbial resources for the development of a new microbial preparation for green prevention and control of melon root-knot nematode disease and crop health promotion, and has a good application prospect.

The present application belongs to the field of microbial pesticides, and relates to a bacillus megaterium NBNC-104 for preventing and treating root-knot nematodes, a compound microbial agent and application thereof. The preservation number of the bacillus megaterium strain NBNC-104 is CCTCC M 20242324. Laboratory bioassay data show that the bacillus megaterium NBNC-104 has high toxic killing activity and repellent effect on southern root-knot nematodes, and has synergistic nematode killing activity after being compounded with bacillus thuringiensis strain NBIN-863 and kunming streptomyces sp. NBNH-006, and the strain can effectively colonize in the rhizosphere soil environment within 30 days under potting conditions. Therefore, the strain has broad application prospects in agricultural production.