735 results about "Root-knot nematode" patented technology

The invention discloses a soil disinfection high-temperature sterilizing desinsection vehicle which comprises a frame crossbeam and frame side supporting plates fixed on both ends of the frame crossbeam; a gear box is fixedly connected below the frame crossbeam; the front end of the gear box is provided with a connecting frame; the rear end of the gear box is provided with a rotary blade shaft; both ends of the rotary blade shaft are supported to the lower end of the frame side supporting plates; the rotary blade shaft is provided with a rotary blade; an electrical heating device with a downward opening is wrapped on the rotary blade. The approximate central part of the frame crossbeam is provided with an inclined upward expansion oil cylinder. Apart from the rotary tillage of the soil, the vehicle kills the germs in the soil and the pests with virus at high temperature produced by the electrical heating device, can effectively kill the germs and pests including root knot nematode and has high operation efficiency; because no highly toxic pesticide is used, public nuisance, such as water pollution, soil pollution, over standard of pesticide residue in agricultural products, etc. brought by the highly toxic pesticide are eradicated.

The invention discloses a strain of efficient bacillus amyloliquefaciens, and a bacterial agent and application thereof, wherein the Latin name of the bacillus amyloliquefaciens is Bacillus amyloliquefaciens; the strain number is HW05; the accession number of the bacillus amyloliquefaciens in China General Microbiological Culture Collection Center is CGMCC No. 10273. The efficient bacillus amyloliquefaciens provided by the invention has the obvious inhibition effects on fusarium oxysporum, phytophthora capsici, ralstonia solanacearum, pyricularia grisea, colletotrichum gloeosporioides and root-knot nematode. Through the single application of HW05, the efficient prevention and control effects can be achieved on leaf surface disease sweet melon powdery mildew, mango anthracnose diseases andmulberry red rust diseases; the HW05 and paecilomyces lilacinus can be matched for preventing and treating soil-borne banana wilting diseases and banana root-knot nematode diseases, and a good synergyeffect is achieved.

The invention discloses a fosthiazate insecticide composition. The composition contains active ingredients and a solvent, and is characterized in that: the active ingredients contain fosthiazate serving as the main active ingredient, wherein the content of the fosthiazate in the active ingredient is 20-100 percent by weight, preferably 30-90 percent by weight, and more preferably 50-60 percent byweight. The invention also discloses application of the composition to preparation of an injection insecticide. The fosthiazate insecticide composition provided by the invention has the advantages ofstrong systemicity, high targeting property, quick action, good prevention and treatment effect on pests living in trunks or underground such as longhorn beetles, pine wood nematodes, coccids, armoured scales and root knot nematodes, death rate of the pests of reaching up to over 90 percent in use by injection, convenience in preparation and use and high effective utilization of the agent in use by injection. The fosthiazate insecticide composition provided by the invention can be widely applied to the field of insecticides of agriculture and forestry such as forests, gardens and fruit trees.

The invention discloses a cultivation method for preventing tomato root-knot nematode worms. Due to the facts that concave-concave type structure cultivation ridges are innovated, and a solar greenhouse tomato special type planting mode is set, organic combination among biological avoidance, biological trapping and biological fumigant is achieved, and the purpose of control over the hazard of the root-knot nematode worms is achieved. According to the cultivation method, no chemical pesticide is used, the root-knot nematode worms can be effectively prevented, the root-knot index of tomatoes is greatly reduced, the yield of the tomatoes is improved, energy consumption is low, and the low-carbon concept is met.

The invention discloses an emamectin benzoate microsphere and a preparation method thereof. The emamectin benzoate microsphere is prepared by utilizing an emulsification-volatilization method. According to the emulsification-volatilization method, solid spherical microspheres are prepared by taking polylactic acid as a carrier and taking gelatin as a dispersant, wherein the microspheres are monodispersed fine powder, and the particle size distribution of the microspheres is uniform. According to the emamectin benzoate microsphere, active ingredients are slowly released, the stability of emamectin benzoate is improved, a persistent effect is long, the work efficiency is high, and a lasting period can reach 3-6 months after the microsphere is applied once. The emamectin benzoate microsphere disclosed by the invention is suitable for being mixed with soil to be applied and can be widely used for treating soil insects and root-knot nematode of various crops.

The invention discloses paecilomyces lilacinus and an application thereof. The provided paecilomyces lilacinus refers to paecilomyces lilacinus E16 with a register number of CGMCC No.5951 in the common microorganism center of the China microorganism culture preservation management committee. According to the paecilomyces lilacinus E16, in potting experiments, the control effect on second-instar banana root-knot nematodes is 86.37%, the growth rate to banana root weight is 37.76%, the growth rate to banana plant height is 56.47%, the growth rate to overground parts of banana is 38.03%; and in area experiments, the control effect on banana root-knot nematodes is 83.03% and the growth rate to banana fruit is 28.05%.

The invention relates to an antibiotic streptomyces, fermentation product of which can inhibit broad spectrum fungus and protozoan, and field experiment can control crop fungus plant disease and root-knot nematode. The antibiotic streptomyces has good effect, no pollution nuisance and no residue. The antifungal agent has extremely low hazardness for the people and good performance and development prospect for medical and agricultural use. The invention is named as SIM001 antibiotic streptomyces which is Streptomyces antibioticus subspecies xi an, and is preserved as patent in the depositary institution appointed by patent office of state intellectual property office; the preservation data is April 14th, 2005, the name of the depositary institution is CGMCC, and the number of preservation is CGMCC No.1349. The whole cell hydrolysate of the antibiotic streptomyces contains L, L-DAP (Diaminopimelic acid) and no characteristic glucide; the cytoderm belongs to I type and glucide type C; one of the main antibacterial materials is polyene macrocyclic ketolide which belongs to the broad spectrum antifungal substance.

The invention relates to bacillus subtilis M3 and an application thereof, and belongs to the technical field of biological pesticides. The produced strain is the bacillus subtilis M3; the preservation unit is the China General Microbiological Culture Collection Center; the address is Zhongguancun, Beijing, China; the preservation date is July 11, 2013; the preservation number is CGMCC NO.7911. The application of the bacillus subtilis M3 as a preparation for preventing and controlling Chinese cabbage clubroot, tomato scab, tomato root knot nematode diseases and konjak bacterial soft rot is also disclosed. The bacillus subtilis M3 disclosed by the invention achieves an obvious and stable prevention and control effect on the diseases, can be used for preparing the preparation for preventing and controlling the Chinese cabbage clubroot, the tomato scab, the tomato root knot nematode diseases and the konjak bacterial soft rot and has the advantages of high efficiency, nontoxicity, safety, no residue, bacteria activation, disease control, better growth promotion effect, better comprehensive character and easiness for industrialized production.

The invention relates to a culture method of a fungus capable of poisoning plant parasitic nematodes, and a preparation method of a metabolite thereof and application thereof, and belongs to the technical field of microbial pesticides. A fungus strain (Aspergillus niger Y-61) related in the invention was collected in China General Microbiological Culture Collection Center on August 7, 2008, and the collection number is CGMCC No.2631. The taxonomic status of the Y-61 is a mitosporic fungus, hyphomycetes, moniliales, moniliaceae, aspergillus and aspergillus niger. The fungus metabolite is prepared by liquid fermentation and culture, has outstanding characteristics of high toxicity to the plant parasitic nematodes, especially root-knot nematodes, and has obvious nematodes killing effect; and the influence of the strain fermentation broth on Meloidogyne incognita juveniles (J2) and oocyst hatching is determined indoors. Treatment of the fermentation broth with different diluted concentrations is significantly different from the aseptic water treatment, and the preventive effect of the fermentation broth with less than one-fifth concentration is be equivalent to that of 10mug/ml of cadusafos. The fungus has good application and development prospects.

The invention relates to the field of fertilizers and provides a special organic fertilizer which comprises the following components in parts by weight: 50-70 parts of organic matter, 20-35 parts of composite amino acid hydrolysate, 2-6 parts of nitrogen fertilizer, 1-3 parts of potash fertilizer and 3-8 parts of phosphate fertilizer. The organic fertilizer is produced by complexing the composite amino acid hydrolysate with sludge, the process flow is reasonable, the operation is simple, the preparation method is suitable for mass production, and a value added is high. The special organic fertilizer is high in activity and rich nutrient, has an effect of efficient soil improvement of an organic fertilizer, can obviously improve disease and insect pest resistance of crops and restore damaged root systems, and can particularly reduce a soil-borne fungal disease and a root knot nematode disease effectively.

The invention belongs to the field of biological prevention and control on crop insect damage and provides a microbial preparation for inhibiting root-knot nematode. The preparation is formed by mixing bacterial liquids of streptomyces avermitilis, paecilomyces lilacinus, verticillium chlamydosporium and Bacillus sphaericus and glycerol. The invention also provides a production method and an application effect of the microbial preparation for inhibiting the root-knot nematode. The microbial preparation comprises viable bacteria of which the number is greater than 2,500 millions per milliliter, wherein the viable bacteria comprise the streptomyces avermitilis, the paecilomyces lilacinus, the verticillium chlamydosporium and the Bacillus sphaericus. After the microbial preparation is prepared into soil, eggs of the root-knot nematode are killed by metabolites generated in the continuous growth and reproduction activity process of the viable bacteria, so that a granular structure of the soil is improved, the utilization rate of a fertilizer is improved and an effect of increasing both production and income is achieved.

The invention relates to a root-knot nematode control agent as well as a preparing method and application thereof, belongs to the field of agriculture, and aims to solve the technical problem that a plant source root-knot nematode control agent as well as a preparing method and application thereof are provided. The root-knot nematode control agent provided by the invention has active constituents prepared from the following plant materials of Chinese herbal medicines in parts by weight: 1-8 parts of peganum harmala, 0-8 parts of folium ginkgo, 1-10 parts of sphora alopecuroide, 0-5 parts of radices sophorae subprostratae, 0-5 parts of rhizoma menispermi, 0-10 parts of radix sophorae flavescentis, 0-8 parts of castor beans, 1-10 parts of abutilon fibers, 0-8 parts of crown daisy and 0-5 parts of mandala. Meanwhile, the invention further discloses a preparing method and application of the root-knot nematode control agent. The root-knot nematode compound has the advantages of safety and nontoxicity, meanwhile can effectively prevent, restrain and kill root-knot nematodes.

The invention discloses a bacillus subtilis bacterial strain and application thereof. The strain is bacillus subtilis Bs-1, is preserved in China Microbiological Collection Management Committee General Microbiology Center, and has a preservation number of CGMCC NO.12364. The invention further discloses application of a biological agent prepared by the bacillus subtilis Bs-1 to preparation and treatment of root-knot nematode. In the invention, bacillus subtilis is separated from soil, has an excellent control effect on root-knot nematode, is easy for colonization in the soil, and is beneficial to early prevention and control of the root-knot nematode hazard; the bacterial strain has a huge application potential for solving the problem of prevention and treatment of the root-knot nematode, and is helpful for implementing high yield, safety and pollution-free production of vegetables; fermentation liquor of the bacillus subtilis Bs-1 bacterial strain reaches a fatality rate of 100% on root-knot nematode, reaches a prevention and treatment effect of 76.9% in a greenhouse, has the good and stable prevention and treatment effect, and is suitable for agricultural production requirements.

The invention relates to a special peanut organic and inorganic compound fertilizer for biologically preventing and treating root knot nematode disease and preparation method thereof. The organic and inorganic compound fertilizer is prepared by carrying out liquid fermentation on actinomycetes for preventing and controlling the root knot nematode disease, mixing with organic matters formed by fermenting and decomposing xylitol used as raw materials and matching with inorganic fertilizer according to the nutrient requirement of the peanut. The special peanut organic and inorganic compound fertilizer has favorable disease prevention effect and safe use, fertilizes soil and can improve the yield of peanuts.

The invention belongs to the technical field of microorganism genetic engineering and discloses separation and cloning of nematicidal crystal protein gene originated from thuringiensis and biological activity of the nematicidal crystal protein gene on nematode. In the invention, a new insecticidal crystal protein gene cry1518-35 is obtained by being separated from bacterial strain of activated thuringiensis YBT-1518 of root-knot nematodes in north of China; an encoded product of the gene is a new insecticidal crystal protein Cry1518-35; the protein Cry1518-35 features high insecticidal activity on root-knot nematodes in north of China. The invention also includes the preparation of colon bacillus recombinant bacteria EMB0225 which expresses the insecticidal crystal protein Cry1518-35, the recombinant colon bacillus is preserved in CCTCC, the preservation number is CCTCC NO: M209009.

The invention discloses a fully degradable seedling-growing integrated nutritional bowl and a production method thereof. According to parts by weight, 20 to 30 parts by weight of crop straws, 15 to 25 parts by weight of cassava residue, 20 to 30 parts by weight of starch residue and 10 to 15 parts by weight of rice bran or saw powder are uniformly mixed and then sufficiently ferment, 10 to 20 parts by weight of zeolite powder, 8 to 15 parts by weight of bentonite and 0.1 to 0.3 parts by weight of chitin are then added into the fermented material and uniformly mixed, the mixture is then put into a mold and hot-pressed to be shaped, and after airing, the fully degradable seedling-growing integrated nutritional bowl is produced. The fully degradable seedling-growing integrated nutritional bowl does not need to be removed after being transplanted, soil pollution is prevented, and meanwhile, the problems of continuous cropping harm, root-knot nematode and soil-borne diseases can be solved.

The invention discloses a bioorganic fertilizer for adsorbing heavy metals and improving greenhouse soil. Raw materials of the bioorganic fertilizer comprise, by weight, 83-88 parts of a decomposed organic fertilizer, 8-12 parts of biochar, 1-2 parts of attapulgite, 2-3 parts of functional bacteria, and 0.5-1 part of methyl salicylate, wherein raw materials of the decomposed organic fertilizer comprise, by weight, 45-60 parts of straws, 30-45 parts of traditional Chinese medicinal residues, 1-5 parts of a urea fertilizer with the nitrogen content of 46wt%, 1-2 parts of a straw fermentation inoculant, 1-2 parts of penicillium oxalicum and 0.5-1 part of brown sugar. The content of organic matters in the bioorganic fertilizer is not lower than 30%, the quantity of effective live bacteria in the bioorganic fertilizer is not lower than 0.02 billions/g, and the bioorganic fertilizer can effectively enrich heavy metals in soil and reduces the content of the heavy metals; and functional bacteria and methyl salicylate can dually defense internally and externally, can effectively inhibit breeding and invasion of root-knot nematodes, also can improve the structure of soil, increases the permeability, improves the pH value, and reaches yield increase, quality improvement and environmental protection.

The present invention provides a nucleic acid construct comprising a cyst and root knot nematode responsive promoter, preferably the Nicotiana Ntcel7 promoter or promoters that hybridize thereto, operatively associated with a heterologous nucleic acid segment that encodes a product disruptive of nematode attack. Plants and plant cells using the same and methods of use thereof are also disclosed.

The present invention relates to nematicides, which are compositions that kill nematodes when placed in physical contact with nematodes. Nematodes do not decompose organic matter, but, instead, are parasitic and free-living organisms that feed on living material. M. incognita is a root knot nematode that has the ability to cause infection in more than 2,000 species of plants. Specifically, the present invention relates to CED-4 peptides that effectively kill nematodes. These CED-4 peptides are segments of the CED-4 protein. This invention is directed to a nematicide composition comprising an effective amount of a CED-4 peptide consisting of no more than 50 amino acids. In one aspect of the invention, the nematicide composition consists of no more than 30 amino acids. In another aspect of the invention, the nematicide consists of no more than 20 amino acids.

The invention relates to a biodegradable agricultural mulching film capable of preventing and controlling root-knot nematodes and a preparation method thereof. The agricultural mulching film is a thin film formed by mixing Chinese medicinal active ingredients capable of preventing and controlling root-knot nematodes with a PBS or PBS copolymer in a mass ratio of (1-500):1,000. In the invention, powder Chinese medicament active ingredients capable of preventing and controlling root-knot nematodes are used as nucleating agent to be mixed with the PBS or PBS copolymer to manufacture the mulching film, and the crystallinity is changed to make the manufactured agricultural mulching film more easily degradable and greatly improve the elongation at break and tensile strength of the agricultural mulching film. The agricultural mulching film has a transparent or semi-transparent property, high root-knot nematode prevention and control performance and over 90 percent lethality; and after the agricultural mulching film is placed in compost for 6 months, 80 percent of the agricultural mulching film can degrade.

The invention relates to preparation and application technology for a waxy bacillus suspension agent. The suspension agent is characterized by comprising the following components of: usually 1 to 1,010 bacterial colonies of living body waxy bacillus per milliliter, 1 to 25 weight percent of dispersing agent, 0.1 to 5 weight percent of thickening agent and the balance of water. The waxy bacillus suspension agent can be used for efficiently preventing and controlling root-knot nematodes of greenhouse vegetables such as watermelons, balsam pears, west melons, cucumbers, tomatoes, towel gourds, celeries and the like and outdoor vegetables. The using method comprises the following steps of: (1) after applying farmyard manure on a soil surface, diluting 1,000 milliliters of the waxy bacillus suspension agent by using 40 to 50 kilograms of water, uniformly spraying the diluted suspension agent on the soil surface, and turning soil; and transplanting crops after 3 to 10 days, wherein the effect of preventing and controlling the root-knot nematodes of the vegetables can reach 70 to 90 percent; and (2) after conventional fertilization of the soil, diluting 6,000 milliliters of the waxy bacillus suspension agent by using water until the diluted volume is 40 to 50 times the original volume of the suspension agent and irrigating roots by using the diluted suspension agent which serves as root-fixing water when transplanting the crops. The waxy bacillus suspension agent can efficiently prevent and control the root-knot nematodes of the vegetables.

The invention discloses a strain of bacillus altitudinis and application thereof in control of tomato root-knot nematode. The bacillus altitudinis strain YT2002 has been deposited in the China GeneralMicrobiological Culture Collection Center (CGMCC) in January 15, 2018 with the Accession No. of CGMCC No. 15213; experimental results show that the bacillus altitudinis strain YT2002 biological agenthas a good effect on control of tomato root-knot nematode, with the control effect of 63.03% after 14 days after the last application; from growth of the tomato, the bacillus altitudinis strain YT2002 has an obvious effect on promoting tomato growth, and is safe and reliable without risk of damage.

The invention relates to a soil disinfector, in particular to a facility vegetable field eelworm killing agent and an application method thereof to solve the problem of preventing and controlling the root knot nematode. The eelworm killing agent comprises the components in weight percentage: 10 to 20 percent of calcium cyanamide and 80 to 90 percent of organic material. The calcium cyanamide and the organic material are mixed and stirred evenly and mechanically to prepare the eelworm killing agent. The organic material is nutshell powder, sawdust or crop straw. The application method comprises the following steps: the vegetable field is cleaned in summer with high temperature and good sunlight, the eelworm killing agent is broadcasted, the subsoiling and the ridge forming processes are performed, a film is covered, and the irrigation is performed; and after the vegetable field is sealed for 14 to 21 days, the film is disclosed, the soil is plowed, and the vegetable field is air-cured for 5 to 7 days. The facility vegetable field eelworm killing agent and the application method thereof can be applied to prevent and control vegetable root knot nematode for more than 30 vegetable crops such as facility vegetable field cucumber, tomato, kidney bean, melon and celery, have simple technique, sufficient raw materials and low price, and prevent and control the vegetable root knot nematode by more than 70 percent.

The invention discloses a method for preparing a bio-organic fertilizer from marigold straws. The method comprises the following steps: A, precrushing; B, mixing auxiliary materials; C, stacking and fermenting; D, crushing; E, inspecting; and F, packaging, wherein in the step B, weight ratios of the marigold straws, organic matters, a microbial agent and water are 10-60 percent, 20-40 percent, 0.05-0.5 percent and 19-50 percent respectively. By the method, the marigold straws which are not utilized for a long time are prepared into the organic fertilizer through a scientific and reasonable formula, so that straw disposing difficulty is solved in the industrial development of marigold as well as an efficient bio-organic fertilizer with an effect of controlling root-knot nematodes, gray molds, molds and the like is provided; and the waste marigold straws are really changed into valuable things; therefore, the method has relatively good social and economic benefits.

The invention relates to a method for cultivating a fungus capable of poisoning and killing plant parasitic nematodes and a method for preparing and using metabolites thereof, belonging to the technical field of microbial pesticides. The Trichoderma strain Snef85 involved in the present invention is Trichoderma harzianum, which has been preserved on October 30, 2007 in the General Microorganism Center of China Committee for the Collection of Microorganisms (address: Datun Road, Chaoyang District, Beijing, postal code 100101), The deposit number is CGMCC No.2235. The taxonomic status of the Trichoderma strain Snef85 is Deuteromycotina, Hyphomycetes, Trichosporales, Trichosporaceae, Trichoderma, and Trichoderma harzianum. It is prepared by liquid fermentation culture, and its metabolites have high toxicity to plant parasitic nematodes, especially root-knot nematodes incognita, rice stem-point nematodes and soybean cyst nematodes, and outstanding nematicidal effect. The fermentation broth of the strain has obvious control effects on root-knot nematode (Meloidogynes pp.), rice stem-point nematode (Aphelenchoides besseyi) and soybean cyst nematode (Heterodera glycines). There are significant differences between the treatment of fermented liquid with different dilution concentrations and the treatment of aseptic water, and the nematode killing effect of fermented liquid can reach more than 95%, which has good application and development prospects.