228 results about "Root-knot nematode" patented technology

The invention provides a spiropolyspora roseus strain for preventing and treating root knot nematode disease, and the preservation number of the spiropolyspora roseus strain is CGMCC No.41822. The invention also provides a microbial inoculum containing the spiropolyspora pink strain, and the microbial inoculum is prepared by the following steps: inoculating a culture medium with the spiropolyspora pink HLF-14-11, and culturing until spores are produced; spores are eluted to prepare a spore suspension, or a bacterial block is cut to serve as a liquid fermentation inoculant; inoculating the inoculant into a liquid culture medium, and carrying out shake culture; and taking the fermentation liquor to prepare liquid, powder and granules. The spiropolyspora pink strain provided by the invention is separated from root-knot nematode oocysts of diseased tomatoes, can parasitize eggs of root-knot nematodes and inhibit incubation of the eggs, fermentation filtrate of the spiropolyspora pink strain has a relatively strong poisoning effect on second-stage larvae of the root-knot nematodes, and the fatality rate of the second-stage larvae reaches 89.1%. Meanwhile, the strain can produce auxin substances such as indoleacetic acid and the like, and plant growth and root development are promoted.

The invention belongs to the field of microorganisms, and particularly discloses a microbacterium paraoxidans strain and application thereof, the microbacterium paraoxidans strain TYQ7 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.27216, and the preservation number is CGMCC No.27216. The microbacterium paraoxidans strain TYQ7 has the advantages that the microbacterium paraoxidans strain TYQ7 is preserved in the China General Microbiological Culture Collection Center (CGMCC); the applicant finds that the microbacterium paraoxidans can secrete auxin, can promote growth of cucumber seedlings, also has the capabilities of interfering tropism migration of nematodes and killing nematodes, has the effect of remarkably relieving stress of root-knot nematodes, also finds that the microbacterium paraoxidans has the capability of inhibiting normal growth of fusarium oxysporum, and has the effect of remarkably relieving stress of root-knot nematodes. Furthermore, the microbacterium paraoxidans have the capability of degrading vegetable straws and the capability of dissolving phosphorus, so that the applicant considers that the microbacterium paraoxidans have a remarkable application prospect in the fields of agriculture and environmental protection.

The invention relates to the technical field of fertilizers and pesticides for agricultural production, and discloses a liquid pesticide fertilizer for preventing and treating root-knot nematodes in a water-fertilizer integrated system and application thereof, and the liquid pesticide fertilizer is prepared from the following components: a plant source nematicide, a biocontrol inoculant, a macroelement and medium trace element fertilizer, a boron cross-linking agent, a modified polycarboxylic acid dispersing agent, a microbial protective agent and the like. The preparation method comprises the steps of biological phase pretreatment, matrix phase construction, oil phase micro-emulsification, multiphase fusion and dynamic crosslinking. Through trehalose gradient permeation pretreatment, the survival rate of biocontrol bacteria in a high-salt matrix is increased; a boric acid ester bond dynamic cross-linked network is constructed by using a variable pH adjusting process, so that the system is endowed with shear thinning characteristics, and the contradiction between suspension stability and drip irrigation fluidity is solved; and the chelating dispersion effect of the modified polycarboxylic acid dispersing agent is matched, so that the generation of precipitates is effectively inhibited, and stable coexistence and synergistic interaction of the pesticide, the fertilizer and the bacteria are realized.

The invention relates to the technical field of organic chemistry, in particular to application of pyrazine compounds in the aspect of killing nematodes. The pyrazine compound has good nematicidal activity on pine wood nematodes, root-knot nematodes and aphelenchoides besseyi, and part of the pyrazine compound also has good activity on root knot nematode diseases caused by the root-knot nematodes, so that the pyrazine compound has good application prospects in the aspect of root knot nematode disease resistance.

The invention discloses nematode-killing streptomyces JXSY860 and application thereof, and belongs to the technical field of microbial pesticides. According to the invention, a new strain of Streptomyces turiscabs JXSY860 is found, the new strain of Streptomyces turiscabs JXSY860 has been preserved in Guangdong Microbial Culture Collection Center on August 8, 2024, and the preservation number is GDMCC No: 64998. The invention also discloses a preparation method of the Streptomyces turiscabs JXSY860. Through verification, the JXSY860 strain provided by the invention has stronger lethal activity on second-stage larvae of root-knot nematode, has stronger inhibition effect on incubation of root-knot nematode eggs, has important application value on prevention and control of root-knot nematode diseases of crops, and can be widely applied to preparation of biological agents for preventing and controlling the root-knot nematode diseases.

The invention provides a preparation method of a root knot nematode disease plant source nematicide, and belongs to the technical field of computational chemistry. The preparation method comprises the following steps: firstly, collecting tobacco plant materials in a root knot nematode disease high-incidence area, drying and crushing; determining optimal extraction parameters through a plant extraction parameter optimization function, and carrying out reflux extraction by taking ethanol as a solvent; filtering and concentrating an extracting solution, and adding an organic silicon surfactant and xanthan gum to enhance the stability; adjusting the pH value and adding a preservative; a high-pressure homogenizer is used for treatment, so that the dispersion uniformity is improved; component analysis and activity evaluation are carried out through a plant nematicidal activity prediction model, and the model is based on a convolutional neural network and a Transform architecture, and accurate prediction is realized through an adaptive attention mechanism; and filling the preparation and storing in a dark place. The whole preparation process realizes standardized control of components, and solves the technical problem of large difference between batches of traditional plant source nematocides.

The invention relates to the technical field of prevention and control of microorganisms and nematodes, in particular to arthrobacter pastoris and application thereof. The arthrobacter pastoris disclosed by the invention is classified and named as Arthrobacter pascens, the strain number of the arthrobacter pastoris is YCH-T2, the arthrobacter pastoris is preserved in the China General Microbiological Culture Collection Center on March 19, 2024, and the preservation number of the arthrobacter pastoris is CGMCC No.30072. The strain has strong lethal activity on second-stage larvae of the root-knot nematode, has strong inhibition effect on incubation of eggs of the root-knot nematode, and has important application value in prevention and treatment of root-knot nematode diseases of crops.

The invention discloses a group of polymorphic molecular markers of tobacco southern root knot nematode disease resistance site Rk1 and application thereof. The invention relates to a group of polymorphic molecular markers of tobacco southern root knot nematode disease resistance sites Rk1, which are positioned in a 1-65Mb region of Chr17 # chromosome of a K326 genome. The primer for detecting the polymorphic molecular marker is a primer for detecting the polymorphic molecular marker, and the nucleotide sequence of the primer is any one of seven groups of primers as shown in SEQ ID No.1-14. The polymorphic molecular marker is applied to identification of tobacco southern root knot nematode disease resistant materials or varieties, or is applied to improvement of tobacco southern root knot nematode disease resistance. The polymorphic molecular marker is distributed in an exogenous translocation chromosome segment, has good polymorphism in a natural population, and can accurately detect the meloidogyne incognita resistant Rk1 gene of tobacco.

The invention relates to paenibacillus strongly ZKSW001 as well as a fungicide and application thereof, and belongs to the technical field of microorganisms. The paenibacillus pumilus ZKSW001 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.36076, the preservation date is September 25, 2025, and the address of the preservation institution is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention also provides application of the paenibacillus strongly ZKSW001 in inhibition of harmful bacteria in soil, prevention and treatment of root-knot nematode and promotion of plant growth. The paenibacillus strongly ZKSW001 disclosed by the invention not only can promote the growth of crops and realize weight loss and efficiency improvement, but also can control the occurrence of soil-borne fungal and bacterial diseases, and can inhibit the growth of root-knot nematode. Chemical pesticide application can be reduced, pesticide residues are reduced, and sustainable development of agricultural economy is promoted.

The invention provides a cape jasmine root-knot nematode prevention and control method, and relates to the technical field of plant protection, the cape jasmine root-knot nematode prevention and control method comprises the four steps of preparation of a compound material, compound synergism with an organic bacterial fertilizer, field application and management after application, and according to the cape jasmine root-knot nematode prevention and control method, ricin in castor seed powder slurry can rapidly paralyze and kill root-knot nematode through direct contact; beneficial microorganisms and metabolites thereof in the fermented gardenia oil meal can induce plant root systems to generate resistance, the tolerance of plants to root-knot nematodes is improved, finally, microorganisms in the organic bacterial fertilizer can inhibit incubation and development of root-knot nematode eggs, the breeding and survival rate of nematodes is further reduced, and the yield of the nematodes is increased. Through the synergistic effect of the castor seed powder slurry, the fermented gardenia oil meal and the organic bacterial fertilizer, a contact killing-resistance induction-ovum parasitism triple prevention and control mechanism is formed, and the prevention and control effect can be well improved.

The application provides a Bacillus thuringiensis strain, and the preservation number of the Bacillus thuringiensis strain is GDMCC No. 67284. The lethal rate of the Bacillus thuringiensis strain provided by the application to second instar larvae of root-knot nematode reaches 98%, the control effect in a pot experiment reaches 60.07%, and the control effects on root rot and wilt are 66.79% and 63.23% respectively. The Bacillus thuringiensis strain is a bacillus isolated from rhizosphere soil, is safe to crops, and has control effects on root rot, wilt and root-knot nematode, and has the safety characteristics in agricultural application.

The invention relates to the technical field of plant growth-promoting bacteria and soil-borne disease biological control, in particular to a bacillus velezensis 1708Y11-01 strain and application thereof. According to the bacillus velezensis 1708Y11-01 and the application thereof, the bacillus velezensis 1708Y11-01 is separated from plant rhizosphere disease-inhibiting soil, and the bacillus velezensis 1708Y11-01 has a very high control effect on fusarium wilt and root knot nematode disease, and can be used for improving the resistance of a plant system, improving the leaf chlorosis of crops caused by disease infection and promoting the growth of the crops. The bacillus velezensis 1708Y11-01 strain provided by the invention is pollution-free, residue-free and eco-friendly in the application process, and is a biocontrol strain with a good application prospect in the field of disease prevention and control.

The invention relates to application of a dazomet liquid preparation in soil treatment in the field of pesticides. The liquid preparation can be used for preventing and treating true and bacterial diseases, nematodes and weeds of crops, and has a remarkable synergistic effect on soil-borne bacteria, fungal diseases, root-knot nematodes and field weeds. The pesticide application mode can be selected from one or more of drip irrigation, flushing application, mist spraying and soil layer treatment, and the pesticide can be directly used or used after being diluted for multiple times during pesticide application. Besides, the core advantage of the application is that the application limitation of the traditional dazomet preparation is broken through, and the dazomet preparation is suitable for wide regions, especially suitable for low-temperature regions and environments with the soil temperature being lower than 10 DEG C, and is especially preferably applied to rice seedling raising fields in low-temperature regions; multiple operation modes such as soil mixing, pulping and rice hull mixing can be adopted in a low-temperature scene, the operation is convenient and fast, the actual field requirements are met, and the environmental adaptability and the application flexibility of the dazomet preparation are remarkably improved.

The invention provides a multifunctional paenibacillus terrae capable of degrading organophosphorus pesticides, resisting diseases and promoting growth, the strain is paenibacillus terrae and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.30973. The invention further provides a preparation method of the paenibacillus terrae. The strain can degrade organophosphorus pesticides: profenofos, malathion, diazinon, phoxim and fenthion, wherein the degradation effect on the diazinon is the best; the growth and development of meloidogyne incognita can be inhibited, and tomato rhizobium caused by the meloidogyne incognita Fusarium oxysporum can be antagonized, and tomato fusarium wilt caused by the fusarium oxysporum can be prevented and treated; the fertilizer can increase the supply of rapidly available phosphorus in soil and promote the growth of leaf vegetables.

The invention relates to the technical field of pest repellents, and discloses an L-tryptophan amide salt tobacco root-knot nematode inhibitor. The structural formula of the active ingredient of the L-tryptophan amide salt tobacco root-knot nematode inhibitor provided by the invention is # imgabs0 #. The L-tryptophan amide salt tobacco root-knot nematode inhibitor provided by the invention has a good inhibition effect on tobacco root-knot nematode, and can effectively block a parasitic channel of the root-knot nematode and restore normal growth of plants; the L-tryptophan amide salt type tobacco root-knot nematode inhibitor provided by the invention has relatively high biological safety.

The invention provides a micro-capsule suspending agent containing fluopyram as well as a preparation method and application of the micro-capsule suspending agent, and belongs to the technical field of pesticide preparations. The micro-capsule suspending agent containing fluopyram is obtained by adopting a suspension polymerization method, and the obtained micro-capsule suspending agent is high in encapsulation efficiency and excellent in dispersity, has good ultraviolet stability and heat storage stability, and has a good prevention and removal effect and a long lasting period when being used for preventing and treating root nematodes. After root-irrigation pesticide application, the micro-capsule suspending agent containing fluopyram provided by the invention can be slowly released in soil and roots, has a good prevention effect on tomato and cucumber root-knot nematode, and has the advantages of long lasting period, multiple prevention and control crops, wide bactericidal spectrum and excellent uptake conductivity.

The invention provides a bacillus velezensis QT1 and an application of the bacillus velezensis QT1. The classification name of the bacillus velezensis is bacillus velezensis QT1, and the bacillus velezensis is preserved in the China Center for Type Culture Collection on November 12, 2025, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20252531. The strain shows remarkable biological control efficiency, realizes effective control on various plant pathogens, and can prevent and treat tomato bacterial wilt and tea tree anthracnose. In addition, the bacillus velezensis QT1 can also effectively kill root-knot nematodes (especially meloidogyne incognita). When the strain is applied to plant cultivation, the growth of various pathogens can be remarkably inhibited, the health and yield of crops are improved, the strain is environment-friendly, the use of chemical pesticides is reduced, soil pollution is avoided, and the development requirement of sustainable agriculture is met.

The application discloses application of 3-(4-fluorophenyl) prop-2-yne carbonitrile in prevention and treatment of plant root knot nematodes and belongs to the technical field of plant root knot nematode prevention and treatment. 50 It is found for the first time that 3-(4-fluorophenyl) prop-2-yne carbonitrile has significant insecticidal activity on southern root knot nematodes, the LC 3.109 μg / mL of 3-(4-fluorophenyl) prop-2-yne carbonitrile on southern root knot nematodes is 72 h, the average mortality reaches 92.5% when the application concentration is 48 μg / mL, and the 3-(4-fluorophenyl) prop-2-yne carbonitrile can be used as a novel and effective drug for plant root knot nematode prevention and treatment.

The present application relates to the field of the prevention of southern root-knot nematode, and discloses a Pseudomonas kribbensis ZJU1, with a preservation number of CCTCC NO: M 20221258.The present application also discloses the use of the Pseudomonas kribbensis ZJU1 in trapping and killing southern root-knot nematode.

The invention provides a root-knot nematode biocontrol peptide, the amino acid sequence of the root-knot nematode biocontrol peptide is HLVPGFTWQHIRDIP, the root-knot nematode biocontrol peptide provided by the invention is derived from bacillus megatherium, can provide a root-knot nematode prevention and control effect which is not inferior to abamectin at low concentration, and has a promoting effect on the growth of roots and leaves of tobacco. The invention also provides a recombinant expression preparation method and application of the root-knot nematode biocontrol peptide.

The invention provides an alkaloid Waltherione S analogue as well as a synthesis method and application thereof, and relates to the technical field of alkaloid Waltherione compounds. According to the present invention, the structures of the Waltherione A and the Antidesmone are simplified, the pyridine and pyridone alkaloid Waltherione S analogue is designed and synthesized, and the synthesized compound has broad-spectrum bacterial killing activity on banana wilt pathogen, wheat scab pathogen, banana anthracnose pathogen, phytophthora capsici pathogen, botrytis cinerea, rhizoctonia solani, rice blast pathogen, mango stem-end rot pathogen and the like, the compound also has good nematicidal activity on root-knot nematodes, provides a favorable candidate agent for natural, safe and low-toxicity innovative pesticides, and is expected to be applied to agricultural production.

The application discloses application of a benzamide compound in prevention and treatment of plant root-knot nematodes. N‑ (2-ethyl-1,2,5-thiadiazol-4-yl)-2-iodobenzamide. 2H The application discloses a benzamide compound N‑ (2-ethyl-1,2,5-thiadiazol-4-yl)-2-iodobenzamide. 2H The benzamide compound has strong toxic killing effect on various plant root-knot nematodes, and has no insecticidal activity on non-plant pathogenic nematodes, and has the characteristics of high efficiency, low toxicity and low residue. The application provides a new candidate compound for the prevention and treatment of plant root-knot nematodes, enriches the structure type of the root-knot nematode killing benzamide compound, and provides a new thought and way for development of a new root-knot nematode killing pesticide.

The application discloses a seedling stage resistance identification method for salvia miltiorrhiza root knot nematode disease and application thereof. After seedling of salvia miltiorrhiza seeds to the 5-6 leaf stage, 2mL of a 2-stage young nematode (J2) suspension with a concentration of 200 pieces / mL is inoculated, and the root knot condition of salvia miltiorrhiza and the influence of different infection durations on the growth condition of salvia miltiorrhiza are investigated after inoculation. The number of root knots of the to-be-tested variety is investigated on the 9th day after inoculation of the nematodes, if the number of root knots is less than 15, the to-be-tested variety enters the next stage of identification, that is, comprehensive trait investigation is carried out on the to-be-tested variety on the 21st day after inoculation of the nematodes, if there is no significant difference between the root fresh weight, the height of the aboveground part and the leaf fresh weight of the to-be-tested variety and those of the non-inoculation group, the to-be-tested variety is preliminarily determined as a resistant variety. The application provides a rapid, simple and efficient indoor identification method for seedling stage resistance identification of salvia miltiorrhiza root knot nematode disease.

The invention discloses an actinoplanes strain and application thereof. In a first aspect of the disclosure, there is provided an actinoplanes strain, which may be a strain MN311411 having a preservation number of GDMCC No: 64167, or a strain having at least 98.65% identity with 16S rRNA of strain MN311411, or a strain having at least 98.65% identity with the 16S rRNA sequence set forth in SEQ ID NO: 1, and / or a strain having at least 95% average nucleotide identity with the genome of strain MN311411. The embodiment of the invention discloses a novel strain. The strain or a product based on the strain has a good effect of preventing and treating soil-borne plant diseases and insect pests such as root-knot nematodes and the like.

The application of microbial agent and biochar in the prevention and treatment of tomato root-knot nematode disease is disclosed, which relates to the technical field of tomato root-knot nematode prevention and treatment. The microbial agent is Pseudomonas plecoglossicida, the dosage of Pseudomonas plecoglossicida is 2 kg per mu, and the application amount of biochar is 1 t per mu. Pseudomonas plecoglossicida is dripped into water by drip irrigation, and is dripped once every 20 days. Biochar is applied as base fertilizer before tomato planting. After data measurement, arrangement and analysis, it is found that in the greenhouse soil infected with root-knot nematode disease in Hexi region of Gansu province, when the dosage of Pseudomonas plecoglossicida is 2 kg per mu, and the application amount of biochar is 1 t per mu, the tomato grows well under the condition, the quality is better, and the root-knot nematode disease has better control effect.

The present invention relates to a compound with excellent nematicidal effect, a method for producing the same, a nematicidal formulation containing the compound as an active ingredient, and a method for controlling nematodes, comprising applying the compound to plants or soil. In particular, the compound of the present invention is useful for controlling root-knot nematodes (Meloidogyne spp.), cyst nematodes (Heterodera spp., Globodera spp.), root-rot nematodes (Pratylenchus spp.), Rotylenchus spp., etc.

The present invention provides a benzoxazine-tioxazafen derivative and its preparation method and application, belonging to the technical field of pesticide compound preparation. The benzoxazine-tioxazafen derivative described in the present invention is a derivative of 3-(5-(thiophene-2-yl)-1,2,4-oxadiazole-3-yl)-2H-benzo[b][1,4]oxazine. The present invention uses tioxazafen as the parent structure and splices active substructures with differently substituted benzoxazine derivatives to synthesize a series of new compounds with novel chemical structures. The compounds can be used to control plant parasitic nematodes such as root-knot nematodes and pine wood nematodes. Bioassay results show that the activity of the benzoxazine-tioxazafen derivative against pine wood nematodes is significantly better than that of the commercial agent tioxazafen and is comparable to that of thiazophos, indicating high commercialization prospects.

The application provides a strain of Rhodococcus ZJUHULJT1 and application of the strain in prevention and treatment of paratyphaceae root-knot nematodes, and belongs to the technical field of biological prevention and treatment of root-knot nematodes. Rhodococcus The Rhodococcus (sp.) ZJUHULJT1 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20251182; the Rhodococcus ZJUHULJT1 can significantly inhibit the infection and reproduction of root-knot nematodes, reduce the number of rice root knots, promote the synthesis of defense-related hormones of rice roots, and improve the disease resistance of rice; through the multiple inhibition of paratyphaceae root-knot nematodes, the Rhodococcus ZJUHULJT1 can be applied to the prevention and treatment of rice root-knot nematodes, has good prevention and treatment effect, and provides a new technical means for the pollution-free reduction of paratyphaceae root-knot nematode diseases.

This invention belongs to the field of microbiology, specifically disclosing a strain of *Mcrobacterium paraoxydans* and its applications. The *Mcrobacterium paraoxydans* strain TYQ7 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 27216. The applicant discovered that the aforementioned *Mcrobacterium paraoxydans* can secrete auxin, promoting the growth of cucumber seedlings. It also has the ability to interfere with the directional migration of nematodes and kill nematodes, significantly alleviating root-knot nematode stress. Furthermore, it was found to inhibit the normal growth of *Fusarium oxysporum*, and further exhibits the ability to degrade vegetable straw and solubilize phosphorus. Therefore, the applicant believes that the aforementioned *Mcrobacterium paraoxydans* has significant application prospects in the fields of agriculture and environmental protection.

The invention relates to the crossing field of agricultural biological control and fertilizer technology, and discloses a compound microbial pesticide fertilizer capable of simultaneously controlling root-knot nematode and soil-borne fungal diseases, the pesticide fertilizer has a core-shell structure, and comprises an inner inorganic nutrition core, a middle isolation buffer layer and an outer functional bacterial layer; the raw materials comprise inorganic nutrition core particles, a biological activation organic carrier, composite functional bacterial powder and a modified slow-release binder. Wherein the binder is prepared from oxidized starch and PVA through borax crosslinking, so that the particle strength is enhanced; the carrier is fermented and activated, and synergistic components such as azadirachtin are added. An organic isolation buffer layer is constructed through a layered coating process, osmotic pressure stress of inorganic salt on microorganisms is effectively blocked, and the problem of strain inactivation is solved; the synergistic compounding of paecilomyces lilacinus, pochonia chlamydosporia, trichoderma harzianum and bacillus subtilis is utilized to realize the comprehensive prevention and control of root-knot nematode and soil-borne fungal diseases, and has the functions of nutrient slow release and crop resistance induction.