32 results about "Root-knot nematode" patented technology

The application provides a Bacillus thuringiensis strain, and the preservation number of the Bacillus thuringiensis strain is GDMCC No. 67284. The lethal rate of the Bacillus thuringiensis strain provided by the application to second instar larvae of root-knot nematode reaches 98%, the control effect in a pot experiment reaches 60.07%, and the control effects on root rot and wilt are 66.79% and 63.23% respectively. The Bacillus thuringiensis strain is a bacillus isolated from rhizosphere soil, is safe to crops, and has control effects on root rot, wilt and root-knot nematode, and has the safety characteristics in agricultural application.

The invention provides a bacillus velezensis QT1 and an application of the bacillus velezensis QT1. The classification name of the bacillus velezensis is bacillus velezensis QT1, and the bacillus velezensis is preserved in the China Center for Type Culture Collection on November 12, 2025, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20252531. The strain shows remarkable biological control efficiency, realizes effective control on various plant pathogens, and can prevent and treat tomato bacterial wilt and tea tree anthracnose. In addition, the bacillus velezensis QT1 can also effectively kill root-knot nematodes (especially meloidogyne incognita). When the strain is applied to plant cultivation, the growth of various pathogens can be remarkably inhibited, the health and yield of crops are improved, the strain is environment-friendly, the use of chemical pesticides is reduced, soil pollution is avoided, and the development requirement of sustainable agriculture is met.

The application provides a strain of protozoan nephrospora NJAU-HN1 and an application thereof, and belongs to the technical field of agricultural microorganisms. The nephrospora NJAU-HN1 has a preservation number of CCTCC NO: C2025172. The nephrospora NJAU-HN1 can directly and effectively inhibit the survival of root-knot nematodes. Application of the nephrospora NJAU-HN1 or a culture thereof can significantly promote the growth of melon plants, increase the fresh weight, dry weight and root length of the plants. The nephrospora NJAU-HN1 provided by the application provides valuable microbial resources for the development of a new microbial preparation for green prevention and control of melon root-knot nematode disease and crop health promotion, and has a good application prospect.

The present application relates to the technical field of microorganism and nematode control, and particularly relates to a joint arthrobacter parsonii and application thereof. Arthrobacter pascens The joint arthrobacter parsonii of the present application is classified as Arthrobacter parsonii, and the strain number is YCH-T2, which has been preserved in the China General Microbiological Culture Collection Center on March 19, 2024, and the preservation number is CGMCC No.30072. The strain has strong lethal activity on the second instar larvae of root-knot nematodes, and has strong inhibitory effect on the hatching of root-knot nematode eggs, and has important application value for the control of crop root-knot nematode disease.

Compositions and methods for conferring nematodic activity to bacteria, plants, plant cells, tissues, and seeds are provided. Furthermore, nematode pest populations, particularly Platylenchus species (Pratylenchus spp.), such as pineapple root-knot nematode (Pratylenchus brachyurus), northern root-knot nematode (Pratylenchus penetrans), scribneri, neglectus nematode (Pratylenchus neglectus), Platylenchus zeae, long-tailed lance nematode (Belonolaimus longicaudatus), Hoplolainus galeatus, Helicotylenchus dihystera, spiral nematode (Helicotylenchus pseudorobustus), or Xiphinema nematode (Xiphinema Methods are provided for killing or controlling populations of Americanum nematodes, root-knot nematodes, false sedge nematodes, soybean cyst nematodes, or lance nematodes. The methods include contacting nematode pests with a pesticidal effective amount of a polypeptide containing a nematode toxin. Furthermore, methods for increasing plant yield by expressing the genes of the present invention in plants are also provided.

Hybrid tomato variety ‘H2249’ is described. The tomato variety is a ground-culture hybrid processing tomato variety suitable for the dual use market with both hand and machine harvest and is adaptable to humid climactic regions such as the Northeastern USA, Portugal and Italy. It is a late season variety with resistance to Verticillium wilt, Fusarium wilt races 1 and 2, root knot nematode, tomato spotted wilt virus, bacterial speck, late blight and moderate tolerance to bacterial spot and early blight.

The application relates to the field of biotechnology and plant molecular breeding, and discloses a tomato JMJC1 gene in improving the resistance of plants to plant parasitic nematodes. The JMJC1 nucleotide sequence of the gene is shown as SEQ ID NO. 1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 2. Research proves that, JMJC1 the gene is strongly induced and expressed by root-knot nematodes, the encoded protein has histone demethylase activity, can specifically remove H3K9me3 and H3K27me3 inhibitory methylation modification, thereby removing the inhibition on downstream resistance-related genes, and activating the high expression of defense genes (such as PDF , PI2 and the like). Phenotype identification shows that overexpression of the JMJC1 gene in tomatoes can significantly reduce the number of root knots, and greatly enhance the defense response and resistance of plants to southern root-knot nematodes.

The present application relates to a kind of efficacy composition for preventing and treating tobacco root-knot nematode, fertilizer synergist and its application, the efficacy composition includes α-triphenyl and slightly fermented fertilizer;The slightly fermented fertilizer is respectively with the stem of continuation of stem, the stem of three-leaf bidens and the stem of dandelion as raw material, after cooking treatment, steam explosion treatment, microbial fermentation liquid soaking treatment, fermentation treatment, dry after the three kinds of fermentation products obtained by mixing are obtained.The slightly fermented fertilizer prepared by using specific preparation process can significantly improve the killing efficiency of α-triphenyl on root-knot nematode, and the combination of α-triphenyl and α-triphenyl can synergize, so that the killing effect of α-triphenyl on root-knot nematode is still very good at low use concentration, and the mortality of root-knot nematode is as high as 90%.

The present application relates to the technical field of cucumber root-knot nematode resistance grade identification scheme design, and particularly relates to a method and system for identifying the resistance grade of cucumber root-knot nematode based on characteristic electrical signals. The method comprises: applying an excitation electrical signal of a preset frequency band to a cucumber plant and collecting a response signal thereof; extracting an electrical characteristic related to the physiological state of the plant from the response signal; inputting the electrical characteristic into a pre-trained resistance grade identification model to directly output the resistance grade of the plant to root-knot nematode. The system is a special device for implementing the method. The present application utilizes the internal correlation between the electrical physiological characteristics of plants and genetic resistance to achieve intelligent identification of the resistance of cucumber to root-knot nematode at the seedling stage in a fast, non-destructive and high-throughput manner, and overcomes the shortcomings of long identification period, plant destruction and poor universality of molecular markers in traditional inoculation identification, thereby providing an efficient and reliable technical means for cucumber disease resistance breeding.

The application discloses application of bacillus velezensis LH-BLS107 (CGMCC No. 34785) or an engineering bacterium modified through natural, artificial mutagenesis or gene editing in biological control of plant nematode diseases. Fermentation culture solution, bacterium powder and / or secondary metabolites of the LH-BLS107 or the engineering bacterium thereof are used in wheat, soybean, rice, fruit trees, vegetables and the like. The fermentation culture solution, the bacterium powder and / or the secondary metabolites can effectively prevent and control wheat cyst nematode disease, soybean cyst nematode disease, rice paratylenchus pseudohollandei disease, fruit tree and vegetable root knot nematode disease, and the mortality of the above-mentioned nematodes can reach more than 95%, and when the fermentation culture solution, the bacterium powder and / or the secondary metabolites are compounded with chemical nematocide, the chemical agent dosage can be reduced by 25%-30%.

The application relates to the field of biological control, and discloses a root-knot nematode biocontrol microcapsule for inducing soybean immune response. The microcapsule is a concentric spherical core-shell structure, and sequentially comprises an active core layer loaded with a nematicidal metabolite, an intermediate barrier layer composed of PLGA, a biocontrol bacteria carrier layer embedded with biocontrol bacteria spores, and a functional protective layer with pH response and light avoidance functions. Through the above structure, the application realizes rapid directional release of the nematicidal component and long-acting slow release of the biocontrol bacteria, builds a composite defense system composed of exogenous killing and endogenous immunity in the soybean rhizosphere, and improves the biological control efficiency and the plant stress resistance level.

This invention discloses a bio-fertilizer and soil improvement method for reducing continuous cropping obstacles in cucumbers. By weight, the bio-fertilizer comprises the following raw materials: 30-40 parts of well-rotted livestock and poultry manure, 20-30 parts of straw or rice husks, 1-2 parts of borax, 2-3 parts of urea, 2-4 parts of ammonium sulfate, 2-3 parts of zinc sulfate, 2-3 parts of magnesium sulfate, 1-2 parts of humic acid, and 1-2 parts of a microbial compound agent. The microbial compound agent comprises 4-6 parts of Bacillus megaterium, 2-3 parts of Bacillus polymyxa, 2-4 parts of Trichoderma echinococcus, 2-3 parts of Trichoderma viride, 3-5 parts of Pseudomonas fluorescens, 4-5 parts of Azotobacter chrysogenum, and 2-4 parts of Candida albicans. Using this bio-fertilizer and soil improvement method for reducing continuous cropping obstacles in cucumbers can significantly reduce the impact of continuous cropping obstacles, not only significantly reducing the incidence of cucumber wilt and root-knot nematodes and significantly promoting cucumber seedling growth, but also significantly increasing cucumber yield.

Hybrid tomato variety ‘H2639’ is described. The tomato variety is a ground-culture hybrid processing tomato variety suitable for machine harvest and is adaptable to hot and dry climactic regions such as California central valley, Chile and Xinjiang. It is a mid-season variety with resistance to verticillium wilt, fusarium wilt races 1, 2, and 3, fusarium crown and root rot, root knot nematode, bacteria speck and tomato spotted wilt virus.

Hybrid tomato variety ‘H2650’ is described. The tomato variety is a ground-culture hybrid processing tomato variety suitable for machine harvest and is adaptable to hot and dry climactic regions such as California central valley, Chile and Xinjiang. It is a mid season variety with resistance to fusarium wilt races 1, 2 and 3, root knot nematode, tomato spotted wilt virus.

The subject invention describes a new method for the detection of root-knot nematodes (Meloidogyne spp.) at the egg stage isolated from soil. The described procedure enables the isolation of the eggs of root-knot nematode species directly from the soil and their subsequent use for their detection and identification without DNA extraction. In the procedure, an enzyme cocktail is used to release the DNA content from the material obtained by a specific purification procedure of the soil being tested and put into a real time PCR reaction, which detects the presence of nematodes of the Meloidogyne species. The procedure described here can be easily modified for the identification of other types of parasitic nematodes such as Globodera spp, Heterodera spp ili Pratylenchus spp.

The application belongs to the technical field of microbial compound microbial inoculants and fermentation, and particularly relates to a preparation method and application of an algal-bacterial compound microbial inoculant against ultraviolet stress. The algal-bacterial compound microbial inoculant is prepared by mixing Bacillus thuringiensis NBIN-863 and microthamnus with a preservation number of CCTCC NO: M 20251992. The microbial inoculant enhances the ultraviolet resistance through algal-bacterial metabolic interaction, the microthamnus provides physical shielding and antioxidant protection, and the bacillus secretes nematocidal substances, thereby achieving the dual effects of preventing and treating root-knot nematodes and improving soil nutrients. In addition, the microbial inoculant is expected to be used for fermenting and preparing a small-molecule acid 3-methylthiopropionic acid which inhibits pests.

The application provides a chitinase-producing bacterial screening method for nematicides, and belongs to the technical field of nematicides.The application adopts high-precision liquid chromatography-mass spectrometry technology to detect enzyme products, establishes an accurate mass spectrometry characteristic database, uses a chitin degradation fitting model to accurately analyze the primary, secondary and tertiary characteristics of mass spectrometry data, replaces inaccurate judgment of artificial experience, constructs an accurate double-layer game mathematical model composed of an upper model with the maximum enzyme activity as the target and a lower model with the optimal killing effect of root-knot nematodes as the target, accurately solves the best enzyme strain screening parameter combination through a multi-objective optimization algorithm, tests the root-knot nematode killing activity of the candidate bacterial strains, selects high-precision screening strains with a mortality rate greater than 85%, and performs morphological identification to establish a bacterial strain preservation library, so that the technical problem that the prior art is often based on artificial experience and has insufficient accuracy is solved.

The application relates to the field of soil improvement, and specifically discloses a soil fumigant for preventing and treating soil-borne diseases and application. The soil fumigant is a sulfuryl fluoride compound, and one fluorine atom in the sulfuryl fluoride structure is replaced by a short-chain alkyl group, a short-chain alkenyl group or a halogenated short-chain alkyl group. The soil fumigant has a broad-spectrum effect on the prevention and treatment of soil-borne pathogenic fungi, bacteria, pythium and root-knot nematodes, is a high-value crop soil-borne disease prevention and control product, and solves the problem of shortage of existing soil fumigants.

This invention discloses a compound microbial preparation for controlling root-knot nematodes and promoting plant growth, as well as its preparation and application. Addressing the technical problems of existing biocontrol agents, such as lack of target optimization based on root-knot nematodes, unclear metabolite profiles, poor synergistic effects, and unstable insecticidal and growth-promoting properties, this invention screens *Polyspora pinki* and *Bacillus belye* for co-culture, and through inter-bacterial interactions, directionally enriches synergistic metabolites such as daidzein, isopentenyl adenine, and melatonin. When applied to the rhizosphere, this preparation reduces the root-knot index of cucumbers by ≥40% and increases the fresh weight of aboveground parts by ≥25%, significantly superior to single-strain or physical mixture systems. Furthermore, the product is flexible in form, possessing both viable bacterial colonization ability and metabolite stability; its activity retention rate is >80% after 90 days of storage at 35℃, making it suitable for various application methods such as root irrigation and seed coating, providing a highly efficient and stable technical solution for the green control of root-knot nematodes.

A Tralopyril derivative and use thereof are provided. The Tralopyril derivative may be used to control plant-parasitic nematodes, such as root-knot nematodes and cyst nematodes. The biological assay results show that, using the foliar spray method at a dosage only one twentieth of that used in the root-irrigation method, Tralopyril-β-aminobutyric acid exhibits higher potted-plant activity against root-knot nematodes in tomato, cowpea, and cucumber than cyclobutrifluram applied at the same concentration via the root-irrigation method. Tralopyril-β-aminobutyric acid also exhibits excellent control efficacy against tobacco root-knot nematodes when applied by both foliar spray and root-irrigation methods. Tralopyril-β-aminobutyric acid also exhibits certain control efficacy against cyst nematodes in soybean.

The application discloses a bacillus licheniformis-derived nematocidal peptide Peptide-4 and application thereof in the prevention and control of root-knot nematode diseases, wherein the amino acid sequence of the nematocidal peptide Peptide-4 is shown as SEQ ID NO. 1. The medicament comprises the above-mentioned nematocidal peptide Peptide-4. The nematocidal peptide Peptide-4 can be used for the green prevention and control of root-knot nematodes and the creation of new germplasm resources of crops resistant to root-knot nematodes, meets the green agricultural development requirements, and can effectively solve problems such as pesticide residues, drug resistance and ecological pollution caused by traditional chemical nematocidal agents.

The application belongs to the technical field of biology, and particularly relates to a bacillus cereus for preventing and treating root-knot nematode and application thereof. The bacillus cereus is named as bacillus cereus ZWL-B-24, is preserved in Guangdong Microbial Culture Collection Center (GDMCC) with an address of 5th floor, building 59, Guangdong Academy of Microbiology, 100, Middle Junli Street, Guangzhou, and a preservation number of GDMCC No: 63680, and a preservation time of July 24, 2023. The bacillus cereus obtained in the application is derived from sweet potato soil, has a strong soil colonization capacity, and due to a long-term coexistence with sweet potato root systems, mutual symbiosis, a series of similar survival mechanisms are formed, and the bacillus cereus is applied to the prevention and treatment of sweet potato root-knot nematode disease, and is more safe and reliable for the growth, edible and processing products of sweet potato.

The application provides application of linear dipeptide Ala-Leu in prevention and treatment of nematodes. The linear dipeptide Ala-Leu from plants has the activity of killing various nematodes, including common root knot nematodes, pine nematodes and other plant nematodes and free-living nematodes such as Caenorhabditis elegans, and can obviously kill insects at a low dose; potting experiments prove that the linear dipeptide can prevent and treat plant diseases caused by nematodes. Moreover, the linear dipeptide Ala-Leu has the advantages of small molecular weight, simple structure, low cost, good heat resistance and stability, and is easy to be degraded in animal cells, is safe for humans and other animals, has no activity of inhibiting the growth of bacteria and fungi, is safe for non-target organisms, does not affect the soil bacterial community structure, and has high application value in development of an environmentally-friendly, safe and efficient new type of nematocide and a new type of agent for preventing and treating plant nematode diseases.

This invention discloses a microbial combination with both disease prevention and growth promotion effects, along with its co-cultivation and application. Addressing the issues of insufficient synergistic effect and the need to improve the biocontrol and growth-promoting properties of existing co-cultivation technologies, this invention screens a specific strain combination (*Polyspora pinkis* NF-06 and *Bacillus belyssus* YB-1652) and employs a corresponding co-cultivation process: a specific semi-synthetic culture medium with soybean meal as the main nitrogen source, followed by simultaneous inoculation and culture. This process stably stimulates the synergistic interaction between the two strains, resulting in a product with a mortality rate of over 95.3% against second-instar larvae of the southern root-knot nematode, and significantly promotes the growth of crops such as tomatoes, achieving a synergistic effect of controlling root-knot nematodes and promoting plant growth.

This invention discloses RKNR3 The application of genes in enhancing plant resistance to root-knot nematodes and a method for improving tomato resistance to root-knot nematodes. The inventors of this invention previously discovered through extensive experiments and data analysis that tomato treatment with *Trichoderma* significantly increases its resistance to root-knot nematodes, and... RKNR3 Significant changes in gene expression levels suggest that this gene may play an important role in tomato root-knot nematode resistance. Furthermore, this invention utilizes virus-induced gene silencing technology to construct a silent gene... RKNR3 The VIGS vector of the gene was transformed into tomatoes and successfully inhibited. RKNR3 Silent plants exhibited significantly increased resistance to root-knot nematodes compared to the control group, indicating that this long non-coding RNA participates in the tomato's resistance to root-knot nematodes. This invention provides a theoretical basis and resistance resources for accelerating tomato disease resistance breeding and enriching the diversity of disease-resistant varieties.

The objective is to provide a method for simultaneously and specifically detecting the major root-knot nematode species in Japan. [Solution] A method for identifying root-knot nematodes, comprising the step of performing a multiplex PCR amplification reaction of a target nucleic acid region of one or more root-knot nematodes selected from the group consisting of sweet potato root-knot nematode, Arenaria root-knot nematode Honshu type, Arenaria root-knot nematode Okinawa type, Java root-knot nematode, and northern root-knot nematode using a primer set consisting of four specific primers.

Hybrid tomato variety ‘H2640’ is described. The tomato variety is a ground-culture hybrid processing tomato variety suitable for machine harvest and is adaptable to hot and dry climactic regions such as California central valley, Chile and Xinjiang. It is a mid / late-season variety with resistance to verticillium wilt, fusarium wilt races 1, 2, and 3, root knot nematode, bacteria speck and tomato spotted wilt virus.