52 results about "Aphelenchoides" patented technology

The invention relates to a natural botanical vermin-proof agent, which is prepared from medicinal plants materials including datura flower, roor of sessile stemona, galangal rhizome, cucurbitacin, asaryl, clove, chestnut bark, aromatic plants, camphor, mentha and boneol.

This small hive beetle trap is constructed using common corrugated cardboard method using natural pheromones and a glue substance that's environmentally safe to the applicator, honeybees and natural pollinators. There are no chemical residues transferred to the honey or beeswax. This small hive beetle trap does not add any extraneous construction to the standard beehive. The trap is placed inside the hive around the perimeter of the foundations. Traps can be used all year long.

The invention provides a phyllotreta striolata insect pest situation investigation method. The method includes the following steps that (1) a defined amount of mixed liquor of 10%-20% of a sodium chloride solution and clear water is put in a water basin; (2) a soil sample is put into the mixed liquor and softly stirred, eggs, deadwood and broken leaves float together to form floats, and soil particles settle; (3) the floats on the surface of the mixed liquor are sequentially screened through multiple layers of sieves, each layer of the sieve is washed with flowing tap water and softly stirred with a brush pen in screening, so the eggs, larvae and pupas of phyllotrta striolata are separated from the soil particles and large impurities, and the soil particles are left in the water basin; (4) the larvae, the pupas and the eggs of the second instar and the third instar and the larvae of the first instar of the phyllotrta striolata are respectively picked out with the brush pen and put into culture dishes. The method and the device have the advantages of being easy to operate, feasible, high in accuracy, and capable of providing precise guide for effectively preventing and controlling the phyllotrta striolata, and slowing drug resistance to a certain degree.

The invention discloses application of a beauveria bassiana strain to prevent and control of pests on food crops and application to preparation of a pesticide for preventing and controlling the pests on the food crops. The food crops are paddy rice, beans, potatoes, highland barley, broad beans or wheat; the pests are lissorhoptrus oryzophilus, corn borers, thorn-sucking insect whiteflies, aphids, pine caterpillars, grubs, locusts, potato beetles, monochamus alternatus, termites, tea lesser leafhoppers and peach fruit borers; a culture method of the beauveria bassiana strain comprises the following steps: inoculating the strain on a culture medium for culturing at the temperature of 25 to 30 DEG C for 5 to 10 days, and then obtaining activated spore powder. The beauveria bassiana strain disclosed by the invention has the advantages of high spore-producing ability, good production character and high growth speed; in addition, the beauveria bassiana strain has the characteristics of high toxicity, high insecticidal speed and environment friendliness; by using an insecticidal fungal preparation produced by the beauveria bassiana strain, the number of the population of the pests such as the lissorhoptrus oryzophilus and the corn borers on the food crops can be effectively controlled.

The invention belongs to the technical field of ecological risk assessment of transgenic crops, in particular to a detection method for evaluating the impact of transgenic cotton on dung beetles. The method is realized through the following steps: by taking Bt transgenic insect-resistant cotton and parents thereof and dung beetles as detection objects, collecting fresh cotton leaves, freezing the cotton leaves at -10 DEG C for 24 hours in a refrigerator, and crushing until the particle diameter is less than 0.03 mm; weighing the crushed cotton leaves and cow dung, and mixing proportionally and thoroughly; loading the resulting mixture into a gauze bag which has a pore size of 0.2 mm, inoculating dung beetles to the gauze bag, and sealing the gauze bag and placing on the ground outdoor; and recording the numbers of beetle eggs, larvae and adult insects on the 7th day, the 14th day and the 21st day respectively, and calculating the egg-laying rate, hatching rate and adult insect death rate. The death rate of adult beetles treated by the Bt transgenic insect-resistant cotton is increased, and the egg-laying rate and the hatching rate are reduced, which indicates that the Bt transgenic insect-resistant cotton has a harmful effect on dung beetles. The detection method is accurate, rapid, low in cost and convenient to apply.

The invention provides a cultivating method of a new species of an anti-late blight and anti-beetle potato, relating to a cultivating method of a new species of the potato with a cell culture technology. The method comprises the following steps of: electrically fusing a protoplast (A) which has anti-late blight gene, anti-beetle gene and anti-oxidation gene, and is separated from the wild species of the potato, with a protoplast (B) of the potato culturing species with wide adaptability to form into a fusion cell with the protoplast A and the protoplast B; generating a callus by means of cell culture, and generating a hybrid test tube plantlet of a regenerated somatic cell by means of induction; and further culturing the tissue culture test tube plantlet, transplanting the cultured tissue culture test tube plantlet into a glasshouse or a land for growing field crops, and generating a test tube first generation somatic cell hybridized potato to obtain the anti-diseases and insect cell hybrid potato, wherein the somatic cell hybridized potato has the good agronomic trait of the potato cultivating species and the anti-late blight and anti-beetle trait. The cultivating method provides a new available gene resource which can be used for improving the quality of the potato and improving the antioxidant activity of the potato cultivating species with the wild species.

An improved poultry bedding litter composition includes byproduct or waste material from citrus juice extraction. The citrus peel byproduct or waste litter containing compositions improve poultry characteristics and enhance feed conversion when compared with traditional poultry bedding litters. They also reduce ammonia formation and are very beneficial in addressing litter beetle problems.

The invention relates to a synergistic pesticide composition containing chlorfenapyr fluorobenzamide bisamide and neonicotinoid insecticide. The active ingredient is a binary compound of the chlorfenapyr fluorobenzene bisamide and the neonicotinoid insecticide. The mass ratio of the chlorfenapyr fluorobenzene bisamide to the neonicotinoid insecticide is 1:50-50:1, the mass ratio of the active ingredient chlorfenapyr fluorobenzene bisamide to the flunicarbamide is 1%-80%, and the rest is an auxiliary component; the dosage form of an insecticidal composition is a water-dispersible granule, wettable powder, a suspending agent, an emulsifiable concentrate, a microemulsion or an an aqueous emulsion, and the composition is mainly used for controlling hemiptera, lepidoptera and coleoptera such ashibiscus, planthopper, whiteflies, spider mites, mites, blind mites, scale insects, mites, cotton bollworm, diamondback moth, grass worms, leaf roller moths, heartworm, potato beetles and the like. The composition expands the insecticidal spectrum of a single agent, and has a significant synergistic effect, and the insecticidal activity is obviously enhanced than the single agent, which reduces the dosage of a pesticide and reduces the environmental pollution.

The invention discloses a biological insecticide capable of reducing cucumber diseases and insect pests. The biological insecticide comprises the following raw materials: 5-7 parts of folium ginkgo, 4-6 parts of soybean oil, 6-8 parts of nux vomica, 8-10 parts of mulberry leaves, 8-10 parts of aloe powder, 2-4 parts of lobelia chinensis, 4-5 parts of smallleaf blueberry fruit, 8-10 parts of honeysuckle, 4-5 parts of dolichos lablab root, 6-8 parts of folium isatidis, 10-15 parts of wax gourd leaves, 6-8 parts of blighted wheat, 2-4 parts of shortstyle cratoxylum leaf root bark, 4-6 parts of sunset abelmoschus seeds, 0.5-1.0 part of sophocarpidine, 6-8 parts of humic acid, 10-15 parts of smectite powder, 5-6 parts of chitosan, 1-2 parts of glycine betaine, 0.5-1.0 part of cypermethrin, and proper amount of deionized water. Compared with the prior art, the biological insecticide has the advantages that the formula is carefully selected; the effective components supplement each other and coordinately act; the biological insecticide has obvious effect on preventing and treating aphid, beetle and other mollusk insect pests; the sterilizing and insect pest eliminating effects are good; meanwhile, the biological insecticide is free from drug resistance and free from residue on cucumber.

The invention provides an attractant used for attracting and controlling lasioderma serricorne. The attractant is an alpha-ionone solution, and a use amount of the alpha-ionone is 1-1000 mg/300 m<2>.The invention also provides a lure used for attracting and controlling the lasioderma serricorne, and the lure includes the above attractant used for attracting and controlling the lasioderma serricorne. The attractant and lure provided by the invention have a specific attracting effect on the lasioderma serricorne, and the alpha-ionone exists in tobacco leaves, so that the alpha-ionone is used asthe attractant, has no pollution to tobacco, uneasily produces drug resistance to pests, and improves the effects of forecasting, predicting, attracting and controlling the lasioderma serricorne.

The invention relates to an insecticide for killing potato beetles, which is prepared from the following components in parts by weight: 50 to 70 parts of bacillus thuringiensis, 15 to 25 parts of beauveria bassiana, 1 to 7 parts of synergist, 13 to 25 parts of dispersant, and 0.1 to 0.6 part of ultraviolet protective agent. The botanical insecticide provided by the invention is used for fruit trees, vegetables and tea trees. The insecticide has the advantages of simple composition, convenient preparation, obvious insecticidal effect and no residues, cannot pollute the soil, water source, fruits, vegetables and the like, and can be degraded in the natural environment.

The invention belongs to the technical field of morphological and molecular combined detection, and discloses a morphological and molecular combined detection method for pine wood nematodes. The method comprises the steps of sample separation, morphological detection of the pine wood nematodes and molecular detection of the pine wood nematodes. A simple pine wood nematode separation device is designed in the invention; saprophytic nematodes and aphelenchoides nematodes are distinguished by adopting the presence or absence of stylets, and morphological identification is carried out according tothe shape difference of the tails and the tips of the female adults of pine wood nematodes and the quasi pine wood nematodes; molecular identification is carried out on the aphelenchoides nematodes,the pine wood nematodes and bursaphelenchus mucronatus by adopting a specific PCR technology; and complete detection technology system details are designed. The same pine wood sample is repeatedly sampled for three times during DNA extraction, so that accidental detection errors during sampling are avoided. The method meets requirements of forestry workers for rapid, efficient and accurate pine wood nematode detection, and provides timely and reliable detection means and technical support for general investigation of pine wood nematode diseases of forestry departments.

The invention relates to an efficient insecticide composition for killing agricultural pests. The efficient insecticide composition comprises the following components in parts by weight: 10-50 parts of benzoy urea, 20-40 parts of azamethiphos and 20-60 parts of thiamethoxam. The efficient insecticide composition disclosed by the invention has a remarkable synergetic effect, is capable of reducing the consumption of active ingredients, lowering the use cost, delaying the generation of pest resistance to insecticides, and effectively reducing environment pollution and pesticide residue, and has a remarkable effect of killing pests such as cossidae, aphids, psylla, grapholitha funebrana treitscheke, potato beetles, flies, cockroaches and various mites; meanwhile, the damage to the environment can be reduced.

The subject invention relates in part to the discovery that BtBooster (BtB) peptides enhance Bt strain and Cry protein toxicity to the lesser mealworm. The subject invention also relates in part to the discovery that Bt tenebrionis producing Cry3Aa crystals and Bt japonensis BuiBui producing Cry8Ca crystals are insecticidal to A. diperinus larvae (darkling beetle or lesser mealworm). The subject invention also relates in part to the discovery that fragments from cadherins of the western corn rootworm and the yellow mealworm enhance the toxicities of Bt tenebrionis and Bt japonensis BuiBui toxicity to coleopteran larvae of the genus Alphitobius. In addition, the subject invention relates in part to the use of cadherin fragments to enhance the toxicities of Cry3Aa, Cry3Bb and Cry8Ca to coleopteran larvae of the genus Alphitobius. The subject invention also relates in part to screening Bt strains and Cry proteins for toxicity to larvae of darkling beetles or lesser mealworm, Alphitobius diaperinus.

The invention discloses an application and a product of dsRNA in potato beetle control, and relates to an application of a gene product in potato beetle control and a derivative product. The dsRNA product has strong stability, mature production technology and low cost, and is an effective cheap 'gene pesticide' product with strong specificity, green and environmental protection. The application ofthe dsRNA product in potato beetle control has great and far-reaching significance in potato beetle control. After a potato beetle pesticide is sprayed on leaves of potato plants at a low dose, the potted potato plants can continuously resist insects for more than 28 days, and the control effect reaches more than 80%; and under the outdoor strong ultraviolet condition, the potato beetle pesticidestill has a strong poisoning effect on potato beetles, the field prevention and control lasting period is about 3 weeks, and the prevention and control effect is gradually reduced from 50% to about 10%.

The invention relates to an insecticide composition containing emamectin benzoate and cyflumetofen. Effective components are the emamectin benzoate and the cyflumetofen which are compounded. The mass ratio of the emamectin benzoate to the cyflumetofen is 1-50:50-1. A preparation includes 1-80% by mass of the effective components which are the emamectin benzoate and the cyflumetofen, with the balance being auxiliary components permissable and acceptable in pesticides. The dosage form of the composition is an emulsifiable solution, a suspension, wettable powder, water-dispersible granules, an emulsion in water and a microemulsion. The composition is mainly used for control of lepidopterous and dipterous pests, such as fourbanded leafroller, tobacco aphid noctuid, cotton bollworm, manduca sexta, diamondback moth armyworm, beet armyworm, spodoptera frugiperda, trichoplusia ni, cabbage argyrogramma agnata staudinger, pieris rapae, hellula undalis, cabbage cross-striped borer, manduca quinquemaculata, potato beetles, epilachna varivestis, rice-stem borers and apple fruit borer.

The present invention relates to an insecticidal composition suitable for use in controlling Colorado potato beetles comprising zeta-cypermethrin and a second insecticide selected from the group consisting of acetamiprid and fipronil.

The invention discloses a biopesticide insect repellant. The biopesticide insect repellant is prepared by the following steps that 300 parts of bamboo essence liquid with the content of 10-15%, 100 parts of pepper essence liquid with the content of 10-15%, 100 parts of pepper essence liquid with the content of 10-15%, 100 parts of tobacco leaf essence liquid with the content of 10-15%, 100 parts of lemongrass essence liquid with the content of 10-15%, 100 parts of garlic essence liquid with the content of 10-15% and 300 parts of margosa oil, even stirring is performed after mixing, and a finalproduct is obtained after 2 hours, namely the biopesticide insect repellant. The biopesticide insect repellant is made from pure natural plants, is reasonable in preparation proportion, is convenientto use and friendly to the environment, has no toxic or side effect on human and livestock, can repel aphids, scale insects, acarids, Japanese beetles, locusts and common injurious insects such as flies, also has a bactericidal effect, can eliminate mould and prevent powdery mildew, or even leaf diseases such as black spot and rust disease.

The fungus Metarhizum anisopliae and the fungus Beauveria bassiana have been found to be effective for control of small hive beetles in honey bee colonies. The control of small hive beetles in honey bee colonies is effected by applying to the beetle, the honey bee hive, the vicinity of the honey bee hive, or the surrounding soils, or the soil in the pan at the hive bottom board, a composition of a miticidally effective amount of Metarhizum anisopliae or Beauveria bassiana.