54 results about "Solanine" patented technology

The invention discloses a potato dregs fermentation energy fodder which can replace bran and a preparation method thereof. The principal raw material of the fodder is potato dregs which is prepared through zymohydrolysis and mixed culture solid-state fermentation by adding accessories of bran, urea, ammonium sulphate, monosodium orthophosphate and bitter salt; the preparation method comprises: preprocessing of material, zymohydrolysis of pectase and cellulase, expanding propagation of strains of production, preparation of liquid seed, mother culture and leaven, fermentation and parching; the invention has the advantages that: the potato dregs fodder prepared through fermentation by adopting the method has the content of crude protein of more than 18 percent, over sized fibres of less than10 percent and ash content of less than 5 percent, the potato dregs fodder can meet the standard of first level wheat bran according to <GB 10368-89 wheat bran for fodder>, has no solanine or other microelements with toxic effects, is rich in multiple Probiotic bacteria and has good flavor, and can be used as energy fodder for fowl and Livestock instead of bran.

Nucleic acid sequences from potato that encode the enzyme β-solanine / β-chaconine rhamnosyltransferase (SGT3) are disclosed. Recombinant DNA molecules containing the sequences, and use thereof, in particular, use of the sequences and antisense constructs to inhibit the production of SGT3 and thereby reduce levels of the predominant steroidal glycoalkaloids α-chaconine and α-solanine in Solanaceous plants such as potato are described.

Nucleic acid sequences from potato that encode the enzyme UDP-glucose:solanidine glucosyltransferase (SGT2) are disclosed. Recombinant DNA molecules containing the sequences, and use thereof, in particular, use of the sequences and antisense constructs to inhibit the production of SGT2 and thereby reduce the level of the more human-toxic of the two predominant steroidal glycoalkaloids α-chaconine and / or increase the level of the more insect-toxic α-solanine in Solanaceous plants such as potato are described.

The invention relates to a method for extracting solanine from potato peels. The method comprises the following steps of: safely and innocuously extracting a crude solanine extract from potato peels by using ethanol with the assistance of ultrasonic wave; and separating and purifying the crude solanine extract by using macroporous absorption resin to obtain a potato solanine extract. The method can be used for safely, effectively and industrially producing the potato solanine extract with a purity specification of 20-35 percent to realize the high-valued utilization of potato resources and the industrial production of serial products. Moreover, the solanine which is a main active constituent of the potatoes is extracted, separated and purified by adopting a chemical technology of natural products and a modernized production technology of traditional Chinese medicine, which sufficiently embodies a green chemical production concept.

A process for synthesizing VK2 from solanesol includes such steps as purifying solaneol, dissolving 2-methyl-1,4-naphthylquinone in methanol, adding cyclopentadiee and Lewis acid or protonic acid, reaction to obtain the compound A (1,4,4a,9a-tetrahydro-9a alpha-methyl-1 alpha, 4 alpha-methyl bridge anthraquinone), dissolving solaneol in hexane, adding PBr3, extracting in petroether to obtain solanylbromine, dissolving it in tetrahydrofuran, dripping in said compound A, adding solvanylbromine, reaction to obtain compound B, dissolving it in toluene, and reflux to obtain VK2.

The invention concerns a method and a sorting apparatus for sorting potato products that are moved in a product flow through a detection zone, whereby irregular products are detected in the product flow and are separated from the product flow, characterised in that a light beam having a wavelength of in between 350 and 450 nm is directed towards said products in said detection zone and wherein the intensity of the light that is irradiated by the products is detected in a detection band from 460 nm to 600 nm, wherein a product is qualified as an irregular product and is separated from the product flow when said intensity is below a predetermined value. Further, the invention also concerns a method and a device for detecting the presence of solanine in potato products by means of fluorescence.

The invention discloses a compound biological insecticide. The compound biological insecticide is prepared from, by mass, 2-6% of chenopodium oil, 6-45% of graceful jessamine herb, 10-35% of taro rhizomes, 5-40% of skunk bugbane, 10-30% of taro stalks, 4-6% of solanine, 1-5% of thymopentin and 2-6% of emulsifying agent. Compared with the prior art, the compound biological insecticide has the following advantages that effective ingredients of multiple plants are compounded, supplement each other, have synergistic effect, have obvious prevention and control effect on aphids, beetles and other worms, the effect can be improved according to the insect pest situation, and the problem that the same insecticide is used for a long period of time and accordingly insecticide resistance is produced is avoided. Due to the fact that all the ingredients exist naturally, the compound biological insecticide can be naturally degraded after a long period of time, no residue exists on crops or no health problem is directly caused.

The invention discloses a method for synchronously extracting, separating and measuring solanine and solanum nigtum polysaccharide in solanum nigrum green fruit, the method comprises the steps that ultrasonic wall-breaking, two aqueous phase extraction and high performance liquid phase are combined, synchronous extraction of the solanine and the solanum nigtum polysaccharide in the solanum nigrumgreen fruit is realized through ultrasonic wall-breaking, separation of the solanine and the solanum nigtum polysaccharide is completed through two aqueous phase extraction, and the contents of the solanine and the solanum nigtum polysaccharide are detected with the high performance liquid phase and a phenol-sulfuric acid method. The method is simple, easy to operate and good in reproducibility and can be used for synchronous extraction and separation of the solanine and the solanum nigtum polysaccharide in the solanum nigrum green fruit, and a new technological means is provided for extracting and separating the solanum nigtum effective ingredients in the whole solanum nigtum herb.

The invention relates to the technical field of agricultural planting, in particular to a cultivation method of salt-tolerant Photinia serrulata, comprising the specific steps of inserting cuttings of shoots of Photinia serrulata in a rooting medium of MS (Murashige and Skoog) and 1.3-1.5 mg / L activated carbon for the purpose of rooting culture, placing in a root enrichment medium of MS and 4-5 mg / L solanine for the purpose of root enrichment culture, placing in a root strengthening medium of MS and 10-12 mg / L vitamin P for the purpose of root strengthening culture, and placing in a medium of seaweed pulp and 0.5-0.8 mg / L metronidazole for the purpose of induced culture. Plants of Photinia serrulata cultivated by the cultivation method have great salt tolerance and have a survival rate of 90.2% and above after being transplanted to saline-alkaline lands.

The invention discloses a high performance liquid chromatography detection method of compound nightshade inflammation-diminishing tablets. The compound nightshade inflammation-diminishing tablets are mainly prepared from nightshades, hydrocotyle sibthorpioides and pithecellobium clypearia; the detection method is used for mainly detecting the content of gallic acid, quercetin, kaempferol, solasonine and solamargine in the compound nightshade inflammation-diminishing tablets. Phenolic acids substances including the gallic acid, the quercetin, the kaempferol and the like, and alkaloids substances including the solasonine and the solamargine in the inflammation-diminishing tablets can be detected by steps; the detection method has high detection accuracy and rapid detection speed, and can be used for determining the content of the inflammation-diminishing tablets; the method guarantees the accuracy and advancement of quality detection standards of the inflammation-diminishing tablets are guaranteed, and can be used as important indexes of quality control of the inflammation-diminishing tablets.

The invention discloses crisp eggplant chips and a preparation method thereof, and belongs to the fields of foods and food processing. The crisp eggplant chips comprise the following raw materials in parts by weight: 25-40 parts of eggplant pulp, 20-35 parts of eggplant pulp granules, 2-4 parts of eggplant peel granules, 1-2 parts of salt, 1-2 parts of monosodium glutamate powder, 3-5 parts of white granulated sugar, 12-20 parts of rice noodles, 5-10 parts of broken green and red peppers, 1 part of edible oil and 2-10 parts of minced garlic or 2-10 parts of a red pepper powder and wild pepper powder mixture. The crisp eggplant chips, prepared by using methods of boiling in a pan with rice wine, pickling and the like, are rich in nutrition, low in salt, sugar and fat, convenient to eat by various people and unique in flavor, and have the light fragrance of the wine and the clean aroma of the rice; certain effective medicinal ingredients such as solanine in eggplants are extracted and are beneficial to health of a human body; the problems of deterioration, rotting and the like of the eggplants caused by improper storage in summer and autumn are solved, the waste is reduced, and the loss of eggplant farmers is reduced.

The invention belongs to the technical field of insecticidal treatment of fruit trees, and particularly relates to an efficient environment-friendly insecticide dedicated to a fruit tree. The insecticide comprises the following raw materials in parts by weight: 18 to 25 parts of a toona sinensis bark extract, 9 to 18 parts of a radix linderae extract, 7 to 15 parts of a radix stemonae extract, 4 to 8 parts of a radix zanthoxyli extract, 9 to 13 parts of solanine, 2 to 6 parts of lemons, 4 to 9 parts of orange peel, 3 to 6 parts of linalool and 2 to 4 parts of nanometer chitosan. According to the insecticide, each active ingredient in the formula complements each other and synergistically acts, so that the insecticide achieves high quick availability and activity and long lasting time and kills ova as well as larvae and adults, the components of the insecticide are nontoxic, residue-free, pollution-free and environment-friendly, and environmental pollution can be avoided; in addition, the product smells special to achieve a desinsectization effect; the one-time killing rate reaches 90 percent or more; the aphid and red spider mite killing rate reaches 96.6 percent or more; moreover, insecticide tolerance of insect pests can be avoided, and the insecticide can be used for 3 to 5 years.

The present invention relates to the use of glycoalkaloids, such as solasonine and solamargine, in treatment of cancer such as skin cancer, and to compositions for use in such treatment. More particularly the present invention relates to providing an improved, substantially stable formulation for the glycoalkaloids which minimises or reduces degradation of these active molecules.

The invention discloses a glutamic acid bacillus GH202103 strain for degrading alpha-solanine as well as a preparation method and application of glutamic acid bacillus GH202103 strain. The glutamic acid bacillus GH202103 strain for degrading alpha-solanine is GH202103, the preservation name is Glutamicibacter halophytocolaoGH202103, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the preservation date is May 31, 2021; the preservation number is CGMCC No.NO22638. The strain provided by the invention is a bacterium capable of degrading alpha-solanine, has the characteristics of strong degradation effect on alpha-solanine and safety to environment, and can be used for biodegradation of alpha-solanine and food safety.

The invention relates to the field of traditional Chinese medicine plantation, in particular to a special vegetable insecticide for honeysuckle planting. The special vegetable insecticide comprises an insecticide body and an auxiliary preparation. The insecticide body comprises, by mass percentage, 15-25% of solanine, 2-9% of nicotine, 11-15% of foldleaf daylily root extract, 2-4% of a suspending agent, 1-3% of surfactant, 25-36% of a mixed vegetable material and the balance water. The auxiliary preparation is a mixture of a copper sulfate solution and a ferric sulfate solution, wherein the mass ratio of copper sulfate to ferric sulfate is 1: 1. The invention further discloses a preparation method of the special vegetable insecticide for honeysuckle planting. The method comprises the following steps of 1, weighing each type of the required materials according to the mass percentages; 2, selecting fresh all-grass of humulus scandens, common crabgrass herbs and black nightshade herbs, and removing and substituting damaged parts; and 3, fully pulverizing herba houttuyniae, all-grass of polygonum hydropiper and folium artemisiae argyi through pulverization equipment. Honeysuckle production efficiency and production quality are improved to a great extent; great functionality is achieved; and the special vegetable insecticide can be popularized on the market conveniently.

The invention discloses colored potato sparkling water and a making method thereof. The method comprises the following steps of mixing 150-250g of fresh colored potato flakes with 8-12ml of lemon normal juice and 40-60ml of pure water, and performing mashing so as to obtain colored potato juice; filtering a supernatant of the colored potato juice to obtain a filtrate; mashing 100-200g of apple slices and 100-200g of pitaya flesh so as to obtain apple and pitaya mixed juice; mixing the mixed juice with the filtrate, adding 500g-600g of cane sugar and 30-50ml of passiflora edulia sims normal juice, performing uniform mixing to obtain mixed liquid, putting the mixed liquid in a fermentation tank, and performing fermentation at room temperature for 5-7 days, so as to obtain fementation products namely the colored potato sparkling water. The obtained products have rich fragrance of passiflora edulia sims, pitayas, lemons and the like, not only have the comprehensive color of the raw materials, but also are shining bright and attractive. The contents of antioxidant active components, such as anthocyan, chlorogenic acid and vitamin C are increased, and the decomposition of solanine is promoted. The colored potato sparkling water is long in quality guarantee period, and is sour, sweet and refreshing in mouth feel.

The present invention relates to a potato derived flavour enhancing composition having a dry matter content of at least 3 wt.% and containing per kg of dry matter: 150-900g of proteinaceous matter; 0-100g of starch; 0-5mg of chlorogenic acid; 0-30mg of glycoalkaloid selected from alpha-solanine, alpha-chaconine and combinations thereof; wherein free amino acids represent at least 80 wt.% of the proteinaceous matter, said free amino acids comprising: a first group of free amino acids selected from asparagine (Asn), aspartate (Asp), glutamate (Glu), glutamine (Gln), pyroglutamate (pGlu) and combinations thereof, said first group of free amino acids representing 45-95 wt.% of the free amino acids; a second group of free amino acids selected from glycine (Gly), leucine (Leu), lysine (Lys), serine (Ser), isoleucine (Ile) and combinations thereof, said second group of free amino acids representing 1 -20 wt.% of the free amino acids; at least 3.5 wt.% Glu; wherein the first group of free amino acids and the second group of free amino acids are present in a weight ratio of at least 4:1. The flavour enhancing composition according to the present invention is obtained by removing most of the starch and protein naturally present in potato, by additionally removing the bulk of glycoalkaloids (alpha-solanine, alpha-chaconine) that are endogenous to potato, and by retaining flavour enhancing components, including glutamate.

The invention relates to a microbacterium oxydans NA2 and application thereof. The preservation number of the Microbacterium oxydans NA2 is CGMCC NO. 18777. The Microbacterium oxydans NA2 is a potatoendophyte separated from potatoes, and grows well on a YIM38 solid culture medium. Experiments prove that by inoculating the Microbacterium oxydans NA2 before the potatoes are stored, the increase ofa toxic substance solanine in the potato storage process can be effectively inhibited, and the use safety of the potatoes is remarkably improved. According to themicrobacterium oxydans NA2 , the lossof the potato industry caused by the increase of solanine content is remarkably reduced in a biological control mode, and the microbacterium oxydans NA2 has practical application value in the aspectsof potato planting, storage and the like.

The invention discloses a nano preservative for potatoes, a preparation method and a use method. The preparation method comprises steps as follows: silicon dioxide is added to ethanol and stirred, anda suspension with concentration being 25-100 g / L is prepared; amino silicone oil is added to the suspension, a mixed solution is stirred, dried and ground to 100-300 meshes, and hydrophobic silicon dioxide, namely, the preservative for the potatoes, is obtained. The amino silicone oil is adopted to modify silicon dioxide, a hydrophobic composite nanomaterial is obtained, the material can endow outer skin of the potatoes with hydrophobic performance and reduce moisture attaching capacity, so that the potatoes are effectively inhibited from sprouting, and content of solanine is reduced. The preparation method of the preservative is simple, the preservative is convenient to store, sprouting rate of the potatoes can be at least reduced by 50% or higher, meanwhile, the use process is simple, and the preservative is just required to be sprayed simply, can be popularized in a large range and facilitates long-term preservation of the potatoes.

The invention relates to application of solanum nigrum effective ingredients to preparation of drugs treating lithiasis. Solanum nigrum herb and fruits are adopted as the raw materials, drying, low-temperature continuous puffing, continuous ultrasonic extraction, and multidimensional flow ultrasonic liquid preparation chromatography purification are carried out to obtain three effective components, i.e. solanine, solasonine and solamargine, the substances are then modified by hydrochloric acid respectively so as to generate hydrochloric acid solanine salts, and the effective components are combined with an appropriate excipient to prepare a tablet, a capsule and a sustained-release capsule for treating lithiasis. The technology provided by the invention has the characteristics of high extraction efficiency, simple and practical purification process, and extensive use of prepared drugs, thus being suitable for industrial production.

A solasodine sulfate is prepared from black nightshade, kangroo apple, etc through extracting in acetic acid. It can be used to prepare the anticancer, anti-inflammatory and antiasthmatic medicines.

The invention discloses a potato dedicated castor oil group pesticide. An effective bactericidal agent is composed of castor oil, tomatine, solanine and aminoglycoside, and a solvent carrier is composed of an emulsifier and a solvent; the drug ratio of the castor oil, tomatine, solanine and aminoglycoside of 6:2:1:1, and the drug ratio of the emulsifier to the solvent is 3:7; the ratio of the effective bactericidal agent to the solvent carrier is 2:7, and the rest component of the pesticide is industrial ethanol. With the castor oil as the main raw material and the carrier, the drug sources are rich, are low in cost and easy to obtain, and residues and hazards are not generated; the potato dedicated castor oil group pesticide has the advantages of rich raw material sources, low price, safety, broad spectrum, high efficiency, low toxicity, low residues, and no pollution; and the potato dedicated castor oil group pesticide has good prevention and treatment effect, is nuisanceless, low in toxicity and simple in process, allows pests not to easily generate resistance, and can be used for industrialized batch production.

The invention provides a quality control method of Carina total alkali preparation, whose characteristic resides in that, the identification method of the preparation are as follows: taking the product containing Carina total alkali 5-15 mg, placing it in 2mL volumetric flask, adding 2mL, ultrasonic treating for 20 minutes to make it dissolving, adding methanol to the scale as the solution on approval; then taking solasonine and solamargine as reference substance, adding methanol to dissolve it then diluting into 2mg / mL solution as reference solution; thin layer chromatography, fetching 5 microlitres of each of the three kinds of solutions, marking it respectively into one silica gel G plant with sodium carboxymethyl cellulose as bond, using chloroform: methanol: water (2-3:1-1.5:0.1-0.2) as developing agent, developing to about 15cm, removing, drying, spraying with paraformaldehyde in phosphoric acid, heating up to 105 DEG C till the dot is clear; in the preparation chromatography, the dot with the same color is displayed in the corresponding location of the reference chromatography. The invention can control the quality of the preparation further for improving preparation safety.

a traditional Chinese medicine patch for softening scars, comprising the following raw materials components: 10-20 parts of orange peel; 10-15 parts of stretched grass; 10-15 parts of solanine; 5-12 parts of mulberry; chestnut leaves 5-12 parts; more than 3-10 parts; 3-8 parts of stone cassia; 1.5-5 parts of pig tooth soap; 1.5-5 parts of calcined oyster; 1-3 parts of chuanxiong skin. The invention provides a traditional Chinese medicine patch for softening scar, which enables the active ingredient of the traditional Chinese medicine to smoothly penetrate the skin barrier layer and is introduced into the deep layer of the skin, so as to fully exert the traditional Chinese medicine patch to soften the scar, ventilate the blood, discharge the turbidity, and correct the skin damage part.

The invention discloses a test strip for detecting solanine and application of the test strip. The test strip comprises a sample absorption pad (1), a conjugate release pad (2), a reaction film (3), a water absorption pad (4) and a bottom plate (7), the reaction film is provided with a detection line (5) coated with a solanine hapten-carrier protein conjugate and a quality control line (6) coated with a goat anti-mouse antibody, and the conjugate release pad (2) is sprayed with a solanine monoclonal antibody-colloidal gold marker. The invention also provides a method for detecting solanine residues in potatoes, eggplants and immature tomatoes by using the solanine test strip. The test strip provided by the invention has the characteristics of simplicity in operation, high sensitivity, high detection speed, low cost and the like, and is suitable for screening and on-site monitoring of a large number of samples.

The invention discloses a test paper plate for detecting solanine in potatoes. The test paper plate comprises a base plate, a lining plate and a protective film, a colloidal gold marking pad, a water-absorption pad, a sample pad and a cellulose acetate membrane are adhered to the lining plate in sequence, the overlapping length of every adjacent part of the colloidal gold marking pad, the water-absorption pad, the sample pad and the cellulose acetate membrane is 0.5-0.8 mm, the colloidal gold marking pad is provided with a monoclonal antibody which is coupled with colloidal gold, the cellulose acetate membrane is provided with a detection line, a quality control line and a false positive marked line, and the sample pad is provided with a filter membrane layer. The test paper plate has the advantages of being high in sensitivity, strong in specificity, low in cost, simple in operation, short in detection time, applicable to units of various kinds, easy to store, and long in shelf date; meanwhile, the operation is simple, fast and safe, a large part of raw materials necessary to the reaction are integrated into a PVC backlining, after a sample is subjected to dripping, an antigen-antibody reaction is quickly conduced on a solid-state membrane, and thus the sample detection time is sharply shortened; in addition, the sample needs no special treatment, and the test paper plate is low in cost, easy to popularize, low in production cost, low in investment and quick in effect.

The invention belongs to the technical field of alkaloid toxin detection, and particularly relates to a method for detecting solanine in food by using a double-optical signal ratio sensor system. The preparation method comprises the following steps: firstly, uniformly mixing AChE, ATCh, MnO2 nanosheets, CDs and ultrapure water, standing and incubating to obtain a solution which is marked as a solution a; preparing solanine standard solutions with different concentrations, respectively mixing the solanine standard solutions with the solution a, then detecting fluorescence signals of the mixed solution at the wavelength lambda1 under the excitation wavelength lambda1 in sequence after standing and incubation, and recording the fluorescence signals as F; recording the second-order scattering signal at the wavelength lambda2 as S, and calculating the peak intensity I = F / S of the second-order scattering signal; establishing a standard curve by taking the concentration of the solanine standard solution as a horizontal coordinate and the peak intensity as a vertical coordinate; by measuring the peak intensity of a sample to be measured and substituting the peak intensity into the standard curve, the content of solanine in an unknown sample can be detected. The detection cost is low, and the sensitivity and accuracy are high.

The invention belongs to the field of regeneration through a plant tissue culture technology, and particularly relates to a potato stem apex culture medium and a preparation method and application thereof. The potato stem apex culture medium is prepared from a 0.8MS culture medium, 150-250mg / L of KNO3, 40 to 100mg / L of Ca(H2PO4)2.H2O, 0.1 to 0.3mg / L of solanine, 0.2 to 0.4mg / L of indoleacetic acid, 0.2 to 0.5mg / L of 6-benzyl adenine, 0.05 to 0.2mg / L of gibberellic acid, 0.5 to 1mg / L glutathione, 25 to 35g / L of sugar cane sugar and 8 to 10g / L of agar. By adopting the potato stem apex culture medium, nutrient substances of the culture medium are enriched, the quality of potato plants is improved, the growing speed of potatoes is increased, the seedling period is shortened, the cost is lowered, and the detoxifying efficiency is increased.

The invention discloses a recyclable method for extracting and separating effective components of solanum nigrum. According to the method, ultrasonic wall-breaking extraction and an intelligent two aqueous phase extraction method are mainly used to realize simultaneous extraction and separation of solanine and solanum polysaccharides. The method has the steps of preparation of a solanine mixture standard solution and a glucose standard stock solution, ultrasonic wall-breaking extraction and separation through two aqueous phase extraction. The method can simultaneously extract two effective components of solanine and solanum polysaccharides in solanum nigrum leaves, and realize the separation and measurement of solanine and solanum polysaccharides. Extraction and separation reagents in themethod can be recycled. According to the method, high-efficiency liquid phases are used to measure solanine, and solanum polysaccharides are measured by an ultraviolet spectrophotometer. The method has the advantages of simple and stable steps and good reproducibility.