19 results about "Solanine" patented technology

The invention provides application of a potato StMYB4 gene in regulation and control of solanine accumulation in potato tubers, the StMYB4 gene is cloned through a sequence published in a potato genome database by taking cDNA of leaves of cultivated species Hubei potato No.3 and AC142 as a template, an overexpression and knockout vector is constructed, genetic transformation is performed on the Hubei potato No.3 and AC142 respectively for transgenic function verification, and the solanine accumulation in the potato tubers is regulated and controlled. It is found that excessive StMYB4 genes can significantly promote synthesis of solanine in potato tubers, and knockout of the StMYB4 genes can significantly inhibit synthesis of solanine in potato tubers. The application of the potato StMYB4 gene to regulation and control of tuber solanine can be used for molecular marker development and low solanine variety breeding.

The invention provides an application of a potato StCRY1 gene in regulation and control of potato tuber solanine and chlorophyll accumulation, cDNA of a cultivated species Hubei potato No.3 leaf is taken as a template, StCRY1a and StCRY1b genes are cloned, a double interference vector is constructed, genetic transformation of Hubei potato No.3 is carried out for transgenic function verification, and the potato StCRY1 gene can be applied to regulation and control of potato tuber solanine and chlorophyll accumulation. It is found that interference of the StCRY1 gene can significantly inhibit synthesis of solanine and chlorophyll in potato tubers, and downstream StHY5 of the StCRY1 gene also has the same function as that of the StCRY1 gene and can regulate two channels of solanine and chlorophyll synthesis. Application of the potato StCRY1 gene to regulation and control of tuber solanine and chlorophyll accumulation can be used for molecular marker development and low solanine variety breeding.

The invention relates to the technical field of solanine cleaning, and discloses a high-temperature solanine peeling and cleaning device for potato processing, which comprises a high-temperature peeling machine and a cleaning machine shell mounted at a discharge port of the high-temperature peeling machine. According to the method, a double-cleaning mode of initial immersion and reset spraying is adopted, the potatoes are fully wrapped by cleaning liquid through immersion cleaning, solanine and impurities on the surfaces of the potatoes are completely infiltrated and dissolved, and a foundation is laid for deep cleaning; according to the spraying type cleaning, a fence frame moves downwards to drive a lifting plate to extrude a lower cavity, filtered cleaning liquid forms high pressure, residual pollutants are accurately flushed through a lateral spray head and a central spray head, the advantages of the two modes are complementary, and the solanine removal rate is greatly increased; meanwhile, when the fence frame moves upwards, the lifting plate is synchronously driven to ascend, the potatoes are exposed to facilitate sampling and detection of solanine residues, the fence frame can move upwards to be in linkage with the negative pressure assembly to pull the center spray head to slide towards the two sides, and feeding and discharging interference is avoided.

This invention relates to the field of agricultural product component extraction technology, and discloses an extraction device for solanine from potato tubers. The device includes a main tank, inside which is an extraction tank. Multiple sets of drainage holes are evenly arranged on the outer wall of the extraction tank. Multiple baffles are slidably connected to the outer wall of the extraction tank, each baffle located on one side of a set of drainage holes. A stirring assembly is installed inside the extraction tank, and a lateral displacement assembly is located at the stirring assembly. The lateral displacement assembly drives the multiple baffles to slide simultaneously along the outer wall of the extraction tank. When the baffles block the drainage holes, the extraction solvent and potato fragments can fully react under the action of the stirring assembly. By controlling the sliding of the multiple baffles along the outer wall of the extraction tank through the lateral displacement assembly, the drainage holes are dynamically opened and closed. Combined with the stirring assembly driving the tank to rotate, residual solution is discharged through the drainage holes under centrifugal force, avoiding residual mixed solution and increasing the extraction yield of solanine.

The invention provides a mechanism for regulating and controlling the content of chlorophyll and solanine in potatoes by StERF1B and StERF2 and application of the mechanism. The invention discovers that the ethylene release amount is positively correlated with the contents of chlorophyll and solanine through light treatment of potatoes, and guesses that an ethylene signal may participate in regulation and control of synthetic pathway related genes of chlorophyll and solanine and further regulation and control of the contents of chlorophyll and solanine. Therefore, StERF1B and StERF2 transcription factors most likely to regulate and control chlorophyll and solanine related metabolic genes are screened from numerous transcription factors through transcriptome sequencing, and experiments such as LUC / REN dual luciferase, yeast single impurity, gel migration and the like prove that StERF1B and StERF2 reversely regulate and control chlorophyll metabolic genes StHEMA, StPPO, StPOR2, StCHLI and StCHLM, and simultaneously positively regulate and control a chlorophyll metabolic gene StTIC; and the StERF1B and the StERF2 are used for reversely regulating and controlling solanine metabolism genes such as StCAS1, StSSR1, StPGA1, StDOX and StGAME12. According to the invention, overexpression vectors of StERF1B and StERF2 are successfully constructed, so that the content of chlorophyll and solanine in potatoes is successfully regulated and controlled.

The present invention relates to the field of biotechnology, and in particular to a MT molecular marker related to a potato tuber solanine content regulating gene and its application, and to determining the potato flesh solanine inhibitory factor by amplification results of a marker detection primer set. StERF9 The ploidy of potatoes can be determined by the type and expression level of the promoter, and the level of solanine in the mature potato flesh can be predicted. This greatly shortens the time required to measure solanine in potato flesh after the potato growth cycle is completed. It is a new breeding method that saves time and is highly accurate.

The invention relates to the technical field of agricultural biology, in particular to application of protein 2370 in degradation of solanine. The amino acid sequence of the protein is shown as SEQ ID NO: 1. The streptococcus lactis strain SKLAN202403XW is obtained through identification and separation in potato planting soil, biological protein is obtained from the strain, solanine can be efficiently decomposed, the content of solanine can be remarkably reduced, the toxicity of potato stems and leaves is reduced, and a foundation is provided for subsequent development and utilization.

The present invention relates to a pharmaceutical composition derived from a withanolides extract comprising withanolides and somedone, wherein the somedone in the extract is standardized in the range of 0.1-30%. The content of withanolide in the composition is in the range of 10-60%. The traditional Chinese medicine composition further comprises withanolide glucoside and drunkenin A. The pharmaceutical composition and a pharmaceutically acceptable excipient are prepared into a dosage form. The invention also discloses a method for preparing the pharmaceutical composition. The compositions are useful for improving memory and cognition when administered to a subject in appropriate doses. The composition can enhance the bioavailability of the components thereof.

The invention relates to the technical field of agricultural biology, in particular to application of a protein 0604 capable of efficiently degrading solanine to degradation of solanine. The amino acid sequence of the protein is shown as SEQ ID NO: 1. The streptococcus lactis strain SKLAN202403XW is obtained through identification and separation in potato planting soil, biological protein is obtained from the strain, solanine can be efficiently decomposed, the content of solanine can be remarkably reduced, the toxicity of potato stems and leaves is reduced, and a foundation is provided for subsequent development and utilization.

The invention relates to the technical field of storage and processing of agricultural products, in particular to a multi-factor synergistic effect method and system for controlling the content of solanine in potatoes, and the method comprises callus treatment, short-wave ultraviolet irradiation and electrostatic spraying of a composite inhibitor in a pretreatment stage, and dynamic regulation and control of temperature, humidity and gas components and ethylene intervention in a storage stage. The system integrates an environment sensor, an intelligent controller and an execution mechanism, and can monitor and feed back adjustment parameters in real time. Experiments show that the method can reduce the solanine content to 18 mg / kg or below, is significantly superior to the prior art, can maintain the quality of the potatoes and prolong the storage period to 12 months or above, and has important application value.

The invention relates to the technical field of agricultural product component extraction, and discloses an extraction device for solanine in potato tubers, the extraction device comprises a main tank body, an extraction tank body is arranged in the main tank body, a plurality of groups of liquid discharge holes are uniformly formed in the outer wall of the extraction tank body, and a plurality of barrier plates are slidably connected to the outer wall of the extraction tank body; the multiple blocking plates are located on one sides of the multiple sets of liquid drainage holes correspondingly, a stirring assembly is arranged in the extraction tank body, a side moving assembly is arranged at the stirring assembly and used for driving the multiple blocking plates to slide along the outer wall of the extraction tank body at the same time, and when the blocking plates block the liquid drainage holes, the liquid drainage holes are blocked by the blocking plates. The extraction solvent and the potato fragments can fully react under the action of the stirring assembly, the side moving assembly controls the multiple blocking plates to slide along the outer wall of the extraction tank body, dynamic opening and closing of the liquid discharging holes are achieved, the stirring assembly is matched to drive the tank body to rotate, the residual solution is discharged through the liquid discharging holes under the action of centrifugal force, and the residual of the mixed solution is avoided; the extraction yield of solanine is improved.

The invention relates to the technical field of biology, in particular to an MT molecular marker HapIII combination for detecting high-content solanine and application of the MT molecular marker HapIII combination, MT molecular markers HapIII-1 and HapIII-2 which are remarkably related to the solanine content of potatoes are obtained, the molecular markers are used for detecting a potato breeding material, and the content of solanine in the potato breeding material is detected. The method can accurately and efficiently predict whether the solanine content of the potato tubers reaches 200 mg / kg or not under the condition that planting is not needed to wait for flowering and fruiting of potato plants, can efficiently assist potato breeding selection, is a novel breeding mode which is time-saving and high in accuracy, and has extremely high economic value.

The invention discloses application of an StDGD1 gene related to potato greening and yield, and belongs to the technical field of genetic engineering. According to the invention, the potato StDGD1 gene is accurately edited by virtue of a CRISPR / Cas9 gene editing technology, so that the potato mutant with remarkable improved characters is successfully created. Under the light condition, the chlorophyll content in the tuber of the mutant is remarkably reduced, the greening degree is remarkably delayed, the shelf life of potatoes is prolonged, and the appearance quality of the potatoes is improved. Meanwhile, the accumulation amount of toxic solanine (including alpha-solanine and alpha-canthine) in the mutant tubers is greatly reduced, and the edible safety of the potatoes is improved. The mutation does not cause adverse effects on the tuber yield while realizing the quality improvement, the yield level equivalent to that of a wild type is maintained, and the research result of the invention provides an important gene resource for cultivating a new potato variety with excellent anti-greening character and stable yield.

The present invention discloses a chemical synthesis method of solanine and its derivatives, belonging to the field of carbohydrate chemistry. A method for preparing a compound shown in Formula I, the method comprising glycosidating compound 4 with compound 3 or 5 under the coordinated catalysis of gold (III) ions and nitrile compounds to obtain compound 16 or 15; and a deprotection step; wherein X is NH or O; wherein the structures of compounds 3, 4, 5, 15 and 16 are as follows: The present invention establishes a glycosidation method under the coordinated catalysis of gold (III) ions and nitrile compounds, efficiently glycosidating compound 4 with compound 3 or compound 5, and obtaining compound 16 or compound 15 in a β-configuration in a high yield.

The invention relates to the technical field of biology, in particular to an MT molecular marker combination for detecting high-content solanine and application, MT molecular markers HapII-1, HapII-2, HapII-1 and HapII-2 which are remarkably related to the solanine content of potatoes are obtained, the molecular markers are used for detecting a potato breeding material, and the content of solanine in the potato breeding material is detected. The method can accurately and efficiently predict whether the solanine content in the potato tubers reaches 200 mg / kg or not under the condition that planting is not needed to wait for flowering and fruiting of potato plants, can efficiently assist in potato breeding selection, is a novel breeding mode which is time-saving and high in accuracy, and has extremely high economic value.

This invention belongs to the field of microbial technology, and particularly relates to a *Panthera* strain N16 and its application in the degradation of solanine. A *Panthera* strain ( Pantoea strain N16, of the Pantotheca genus (sp.) Pantoea The preservation number of strain N16 (sp.) is GDMCC NO: 67883. The Pantotheca strain N16 provided by this invention can simultaneously and efficiently degrade α-solanine and α-carboxine, exhibits no hemolytic activity, does not carry resistance plasmids, and maintains high degradation activity in the 30-45 °C range. It can be widely applied in potato food processing, feed detoxification, and storage quality control, providing a safe, efficient, and low-cost microbial resource for the remediation of solanine pollution.

The invention provides a method for controllably extracting alpha-solanine and alpha-canthine from solanaceae plants, which comprises the following steps: crushing solanaceae plant raw materials, extracting with an acidic alcohol solution, adjusting the pH value to be alkaline, collecting precipitates, decoloring and removing impurities with an alkaline alcohol solution, and drying to obtain the alpha-solanine and alpha-canthine. And the ratio of alpha-solanine to alpha-canthine in the extract is regulated and controlled by regulating the ratio of the alkaline solution to alcohol in the alkaline alcohol solution. The method is high in extraction rate and simple to operate, the obtained extract is relatively high in purity, the ratio of alpha-solanine to alpha-canthine is controllable, and the use requirements of various application scenes can be met.

The invention relates to a nitrogen regulation cultivation method for improving alkaloid content of black nightshade green fruits, and belongs to the technical field of planting. A nitrogen regulation cultivation method for increasing alkaloid content of black nightshade green fruits comprises the steps that mixed nitrogen fertilizer containing ammonium nitrogen and nitrate nitrogen is applied to black nightshade plants in stages, and the molar ratio of the ammonium nitrogen to the nitrate nitrogen is 7: 3-3: 7 according to the nitrogen element. The cultivation method is simple in application and low in cost, and the cultivation fertilization mode can remarkably promote the content of solasonine and solamargine in the black nightshade green fruits, so that high-quality medicinal black nightshade fruit resources are obtained.

A method for inhibiting potatoes from greening and use thereof, which can effectively inhibit potatoes from post-harvest greening and production of solanine. The use of 1-MCP for inhibiting potatoes from greening significantly delays the post-harvest greening progress of the potatoes and is of great significance for storage and quality maintenance of potatoes. 1-MCP can inhibit the expression of key genes in the solanine synthesis pathway, inhibit the expression of key genes in the chlorophyll synthesis pathway, promote the expression of chlorophyll degradation genes. It does not affect the expression of germination-promoting genes. 1-MCP can inhibit greening without affecting potato germination, and the potatoes will not be greened even if they sprout. NaOH and 1-MCP are employed synergistically for fumigation to significantly improve the effect of 1-MCP in inhibiting potatoes from greening.