12 results about "Glycoalkaloid" patented technology

The invention belongs to the technical field of agricultural biology, and particularly relates to a transcription factor StBSB with a regulation effect on steroid glycoalkaloid accumulation of potatoes and application of the transcription factor StBSB, the transcription factor StBSB is encoded by a potato gene Soltu.DM.05G001620, and the nucleotide sequence of the potato gene Soltu.DM.05G001620 is shown as SEQ ID NO.1. The invention further discloses a preparation method of the transcription factor StBSB. According to the application, a new insight is provided for understanding a transcriptional regulation mechanism of SGAs in solanaceae plants, and a key theoretical basis and genetic resources are provided for directionally improving the content of anti-nutritional compounds through genetic means.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an application of SlERF098 protein and a coding gene thereof in regulating and controlling steroid alkaloid content to influence botrytis cinerea resistance. According to the application, the resistance of tomatoes to gray mold is enhanced by regulating and controlling the metabolism of steroid glycoalkaloid through the coding gene. The tomato SlERF098 gene is positioned through whole genome association analysis, and an overexpression strain and an RNA interference strain of the tomato SlERF098 gene are constructed. Experiments prove that the SlERF098 can obviously activate the expression of the GAMEs gene in the SGAs metabolic pathway. In addition, overexpression of the SlERF098 gene can improve the content of SGAs metabolites in tomatoes and the resistance to gray mold, while RNA interference strains present an opposite trend. The invention provides a new target for research on disease-resistant molecular breeding and plant immunity-metabolism coordinated regulation and control, and has important agricultural application value.

The invention discloses a compound microorganism fermented attenuated aroma-enhanced potato residue feed and a preparation method thereof, and belongs to the field of feed processing. The feed comprises potato residues, crop straw particles, corn flour, bran, soybean meal, urea, ammonium sulfate and a compound microbial agent, wherein the compound microbial agent comprises bacillus velezensis and lactobacillus plantarum. The preparation method comprises raw material pretreatment; preparing a microbial agent; performing mixed fermentation; and post-processing. The bacillus velezensis secretes glycosidase, and glucosidic bonds of the glycoalkaloid are subjected to enzymolysis; lactobacillus plantarum is metabolized to generate lactic acid to create an acidic environment to promote chemical degradation of glycoalkaloid, and the double bacteria cooperate to inhibit growth of mould, so that efficient attenuation is realized. The bacillus velezensis metabolizes to generate glucose, ethanol and free amino acid, and the lactobacillus plantarum metabolizes to generate ester flavor substances, so that the feed is endowed with strong fermentation aroma. Meanwhile, the pungent smell of volatile aldehyde substances is eliminated through double-bacterium synergism.

The invention belongs to the technical field of agricultural biology, and particularly relates to a transcription factor BSB2 with a regulation effect on steroid glycoside alkaloid accumulation of potatoes and tomatoes and application of the transcription factor BSB2. Comprising StBSB2 from potato and homologous transcription factor SlBSB2 from tomato; wherein the StBSB2 in the potatoes is coded by a potato gene Soltu.DM.04G032190, and the nucleotide sequence of a coding region of the potato gene Soltu.DM.04G032190 is as shown in SEQ ID NO. 1; the SlBSB2 in the tomato is coded by the tomato gene Solyc04g077480, and the nucleotide sequence of the coding region of the tomato gene Solyc04g077480 is as shown in SEQ ID NO. 2. It is clear that BSB2 can form a transcription complex with GAME9 and BSB, expression of downstream SGAs synthesis related genes is activated under stimulation of optical signals, and therefore synthesis of main SGAs in potatoes and tomatoes is positively regulated and controlled. By regulating and controlling the expression and the function of the BSB2 gene, the directional improvement of the steroid glycoalkaloid content in solanaceae plants can be realized. The application provides a new insight for understanding the transcriptional regulation mechanism of SGAs in solanaceae plants, and also provides a new target for potato postharvest quality maintenance and molecular breeding.

This invention belongs to the field of plant genetic engineering technology, specifically relating to the application of the SlERF098 protein and its encoding gene in regulating the content of steroidal alkaloids and influencing resistance to gray mold in tomatoes. The application involves the encoding gene enhancing tomato resistance to gray mold by regulating the metabolism of steroidal glycoside alkaloids. Genome-wide association analysis was used to locate the SlERF098 protein in tomato... SlERF098 Genes, and constructed SlERF098 Overexpression lines and RNA interference lines were observed. Experiments confirmed this. SlERF098 It can significantly activate the metabolic pathway of SGAs. GAMEs Gene expression. Furthermore, overexpression... SlERF098 Genes can increase the content of SGAs metabolites and resistance to gray mold in tomatoes, while RNA interference lines show the opposite trend. This invention provides a new target for molecular breeding of disease resistance and research on the synergistic regulation of plant immunity and metabolism, and has important agricultural application value.

This invention relates to the application of glycoside alkaloids in the prevention and / or treatment of plant bacterial blight, belonging to the field of agricultural technology. The glycoside alkaloids described in this invention have one of the following chemical formulas: The glycoside alkaloids described in this invention can act on the leaves of rice seedlings during the tillering stage, thereby achieving effects such as inhibiting disease and improving disease resistance. This method is simple and inexpensive. The rice bacterial blight inhibitor described in this invention can significantly inhibit yellowing, lesions, and curling of rice leaves caused by pathogens, thereby improving the disease resistance of rice.

The present invention relates to: a gene-edited plant in which expression of StGAME4 and StSGT3 genes is attenuated; a manufacturing method therefor; a guide RNA targeting Solanaceae plant genes or a gene encoding same; and a composition for editing Solanaceae plant genes.

The invention discloses a preparation method and application of potatoes with low glycoalkaloid content, and belongs to the technical field of plant breeding. According to the preparation method of the potatoes with the low glycoalkaloid content, the new potato resources with the low SGAs content are prepared according to the method, the SGAs content in leaves of the potatoes is low, the potatoes can be developed for eating or feeding, and the overall application efficiency of the whole plants of the potatoes is improved. According to the method, the shelf life of the fresh potatoes is greatly prolonged, and the eating safety is guaranteed; and meanwhile, the change of a storage mode is promoted, the potatoes can be stored at normal temperature without light shielding, the energy consumption and the production cost are greatly reduced, and the method has a good application prospect.

The invention relates to a novel spermidine glycoalkaloid compound as well as an application and a preparation method of the novel spermidine glycoalkaloid compound. The novel compound provided by the invention comprises three compounds (capparisane D, capparisane E and capparisane F), is obtained by extracting and separating from a cauliflower (capparaceae) caper vine plant caper spinosa, and is spermidine alkaloid containing a glycoside structure. Wherein the cappissane F is a glucosinolate-containing spermidine alkaloid which is separated from a natural product for the first time, and a biological activity experiment shows that the cappissane F has beta2-adrenergic receptor antagonistic activity and can be applied to medicines for preventing and treating diseases related to the receptor, such as rheumatoid arthritis, arrhythmia and the like.