115 results about "Under-stimulation" patented technology

An artificial induction agarwood production method from a tree of Aquilaria sinensis (Lour.) Gilg by high-pressure injection includes: inducer solution is instantly injected into a trunk of the tree of Aquilaria sinensis (Lour.) Gilg, the inducer solution is led to the tip of the tree through conducting tissues of the tree, the tree of tree of Aquilaria sinensis (Lour.) Gilg rejects drugs under stimulation of inducer and starts defense reaction to generate secondary metabolites, and finally agarwood formation is realized in the trunk under induction. The artificial induction agarwood production method is simple in process and convenient in operation, use of a high-pressure truck injector greatly shortens injection time of the inducer solution, and accordingly the operation is complete in one to two minutes and insusceptible to weather and wind force. Agarwood formation is effective, large-scale standard production is facilitated, and important economic benefit and great ecological benefit on protection of tree of Aquilaria sinensis (Lour.) Gilg and development and production of agarwood are achieved.

The invention discloses double-network ionic gel with stimulation responsiveness and self repairability. Ionic liquid serves as a substrate, P(VDF-co-HFP) is used as a physical cross-linking network,dynamic covalent bond cross-linked PMA (polymethacrylate) copolymer serves as a chemical cross-linking network, and the two polymer cross-linking networks penetrate mutually to form a double network.Under synergistic effects of the double network, the ionic gel is excellent in mechanical performance, thermal stability and ionic conductivity. In addition, due to containing of reversible dynamic covalent bonds, self repairability under stimulation of light, thermal and the like can be realized. The ionic gel can be widely applied to lithium ion battery solid-state polyelectrolyte materials, gasseparation membranes, flexible electric devices, electrochemical sensors and the like.

The invention discloses a fluorescent endoscope imaging system and method, which can be used together with most of the conventional fluorescent probes to accurately excite and develop the distribution of fluorescent probes of various disease targeting tissues in real time. The fluorescent endoscope imaging system is provided with a light source part, an optical signal conduction and acquisition part, an image acquisition part and an image denoising and displaying part, wherein the light source part can generate white light and multispectral excitation light matched with the absorption spectrum of the fluorescent probes; the optical signal conduction and acquisition part can be used for conducting the excitation light or the white light onto cavity tissues in a human body, uniformly irradiating the excitation light or the white light onto a detection target, collecting white light and excitation light diffused by a cavity surface in the imaged human body and fluorescent signals emitted under stimulation and conducting an optical signal to the outside of the body; the image acquisition part can be used for acquiring an original fluorescent image, an excitation light image and a white light image in sequence; the image denoising and displaying part is used for removing noise produced by spectrum aliasing in the fluorescent image and displaying a denoised fluorescent image and white light image.

The invention relates to a method for the preparation of efficient antigen-presenting human Gamma Delta T cells, to the Gamma Delta T cells prepared by such a method, and to their use in immunotherapy, vaccination, vaccine development and diagnostics. Similar to dendritic cells (DCs) in potency and efficacy, these human Gamma Delta T cells process antigens and present antigenic peptides to Alpha Beta T cells and induce antigen-specific responses (proliferation and differentiation) in nave Alpha Beta T cells. Gamma Delta T cells are easily purified from peripheral blood, acquire''maturation'' status (expression of essential adhesion, co-stimulatory and major histocompatibility complex molecules) within 1 day of in vitro culture under stimulation and induce strong primary and secondary T helper cell and cytotoxic T cell responses. The Gamma Delta T cells may be used in a method of treatment of tumors or chronic or recurrent infectious diseases, in identification of novel tumor or pathogen-derived antigens, and in the diagnosis of the immune competence of a patient.

The invention relates to a preparation method for bionic nano-silver. Silver nitrate and natural polymer or derivative of the natural polymer are raw materials and react in a water solution under stimulation of sunlight or visible light. The natural polymer is protective agents, reducing agents and photosensitizer and generates the nano-silver under stimulation of sunlight or low-frequency visible light at the room temperature. The prepared nano-silver has high safety under the protection of the natural polymer, can be applied to biomedical materials or daily chemical products, gives play to an antibacterial function, and can obviously reinforce the Raman signals of molecules to be detected when used as Raman base materials. The preparation method for the bionic nano-silver is simple, reliable, capable of meeting the requirements for the shape and performance of the nano-silver in the field of biomedicine or spectrums, good in biosecurity, capable of controlling the shape and the particle size of products, and wide in application range.

The invention provides a brain-computer interface based doctor-patient interaction method, which adopts a brain-computer interface based doctor-patient interaction system comprising an electroencephalographic collection module and an electroencephalographic analysis and doctor-patient interaction module. The method comprises the following steps of: setting visual stimulation signals with different frequencies, displaying the signals on a display, and mapping into controls of four commands through different combined codes (00, 01, 10 and 11) of 0 and 1; collecting an electroencephalographic signal of a subject by an electrode in real time, sending into a computer after the signal is amplified; analyzing the electroencephalographic signal; and broadcasting a voice prompt corresponding to the identification result through a speaker, and displaying the result on the display. Therefore, medical staff can carry out corresponding rescue according to the display and voice. The brain state can be identified by the brain-computer interface technology, and external equipment is accurately driven in time, so that communication and control can be realized. Brain can be effectively excited to generate steady state visual evoked potential by visual stimulations in different frequencies, the electroencephalographic signal can be accurately identified under stimulations in different frequencies through signal calculation and processing functions of the computer, and real doctor-patient interaction can be realized in the result display and voice prompt mode.

The present invention discloses a near-infrared signal-controlled transcranial magnetic stimulation device, comprising a positioning cap, a fNIRS signal acquisition system arranged on the positioning cap, a TMS system and an analysis control device which is connected to the TMS system and the fNIRS signal acquisition system. The near-infrared signal-controlled transcranial magnetic stimulation device utilizes the near infrared signal of the subject to set personalized transcranial magnetic stimulation parameters, avoiding over stimulation or under stimulation. The near-infrared signal-controlled transcranial magnetic stimulation device is simple and easy to operate, is easy to adjust transcranial magnetic stimulation parameters according to the real-time near-infrared signal in transcranial magnetic stimulation to meet the demand of personalized treatment, enhances the stimulation effect of transcranial magnetic stimulation and expands the application scope of near-infrared signal detection, so as to provide a new method and practical tool for neuroscience research, testing and treatment.

The invention discloses a reduced responsive drug-drug conjugate preparation method which is characterized in that the method includes the steps: preparing a camptothecin intermediate (CPT-SS) containing a reduced responsive bond; preparing a reduced responsive drug-drug conjugate (GT-CPT) or (BT-CPT). The reduced responsive drug-drug conjugate has advantages of a small-molecule prodrug and a nano-drug, the uploading capability of the reduced responsive drug-drug conjugate can be effectively improved, the reduced responsive drug-drug conjugate solves the problems of targeting delivery of a current small-molecule prodrug delivery system and controlled release of drug selectivity, and accurate diagnosis and efficient treatment of tumors are facilitated. Gemcitabine (drug) and biotin (target)molecules are introduced, fluorescence of drug molecules can be temporarily shielded, the conjugate is combined with specificities of cancer cells, so that nano-micelles are disassembled and assembled, the fluorescence of the drug molecules can be reduced, and a drug releases drug properties under stimulation response, so that accurate diagnosis and treatment of cancers are combined.

The invention provides an anti-counterfeiting ink which comprises a Raman mark material having Raman activity. The ink can detect an obvious Raman characteristic scattering peak of a marker material under stimulation of a 514.5 nm light source. The characteristic peak has uniqueness, can be identified and marked, can be distinguished from other components in the ink, and is not interfered by other components, so that the anti-counterfeiting ink can be used for identifying the true and false of paper money and negotiable securities, and has a certain anti-counterfeiting function.

The invention relates to an image attention semantic target segmentation method based on fMRI visual function data DeconvNet. A depth convolution neural network model is trained by fMRI visual functional data collected under stimulation of a natural scene image by a subject and the data are mapped to attention target category tags for model optimization. A depth network model which is symmetrical to the optimized depth convolution neural network is constructed. Parameters of the depth network model are optimized by means of the fMRI visual function data and a semantic segmentation result corresponding to a stimulation image to obtain the mapping of the fMRI visual function data to pixel-by-pixel semantic segmentation results. The fMRI visual function data of a test image viewed by the subject are collected, a subject attention target category and pixel-by-pixel semantic segmentation result is determined, and an attention target region and corresponding target semantics are segmented. The method analyzes the fMRI visual function data which are triggered when the natural scene image is viewed by the subject, extracts all the target categories in the stimulation image and obtains the semantic segmentation result, and improves the accuracy of attention target extraction.

The invention relates to a micro-fluidic chip-based electric field response micro valve and a preparation method thereof. A micro-structure and a micro-passage are arranged on the surface of a micro-fluidic chip; the main micro-passage is provided with a hydrophilic / hydrophobic gate controlled switch; the surface of the micro-passage is modified through electric responsive molecules; change in surface wettability is caused by reversible changes of surface topography and chemical configuration of the micro-passage under stimulation of an external electric field; and motion of micro-fluid on the surface of the micro-passage is controlled, so that an intelligent micro-valve for controlling the flow of micro-fluid is realized. The valve is mainly applied to the related fields such as electrophoretic separation, chromatographic separation and immunoassay. The micro-fluidic chip realizes intelligent driving and control of the micro-fluid, the micro-fluid driving and control cost is greatly reduced, the valve has the characteristics of portability, economy, quickness and high efficiency, and a completely new analysis technology is provided for micro-fluid driving and control.

The invention discloses a multi-harmonic multi-input multi-output feedforward self-adaptive controlling method for helicopter body vibration and belongs to the field of active controlling of helicopter vibration. Aiming at the characteristic of helicopter body vibration under stimulation of the rotor vibration load with multi-stage harmonic frequency and multiple directions and based on the minimum mean square error method and harmonic coefficient identification, the multi-input multi-output feedforward self-adaptive controlling method of helicopter body vibration under the multi-harmonic and multi-load simulation is put forward, so that the purpose of reducing helicopter body vibration is achieved, and when the vibration phase, vibration amplitude and vibration frequency are changed, the good self-adaptive controlling performance is achieved.

The invention relates to a micro-fluidic chip-based pH (Potential of Hydrogen) response micro valve and a preparation method thereof. A micro-structure and a micro-passage are arranged on the surface of a micro-fluidic chip; the main micro-passage is provided with a hydrophilic / hydrophobic gate controlled switch; the surface of the micro-passage is modified through pH responsive molecules; change in surface wettability is caused by reversible changes of surface topography and chemical configuration of the micro-passage under stimulation of different pHs; and motion of micro-fluid on the surface of the micro-passage is controlled, so that an intelligent micro-valve for controlling the flow of micro-fluid is realized. The valve is mainly applied to the related fields such as electrophoretic separation, chromatographic separation and immunoassay. The micro-fluidic chip realizes intelligent driving and control of the micro-fluid, the micro-fluid driving and control cost is greatly reduced, the valve has the characteristics of portability, economy, quickness and high efficiency, and a completely new analysis technology is provided for micro-fluid driving and control.

In certain embodiments, methods of the present invention obtain dynamic data sets of an NMR spectroscopy signal of polarized 129Xe in a selected structure, environment, or system. The signal data can be used to evaluate: (a) the physiology of a membrane or tissue; (b) the operational condition or function of a body system or portion thereof (when at rest or under stimulation); and / or (c) the efficacy of a therapeutic treatment used to treat a diagnosed disorder, disease, or condition. Thus, the present invention provides methods for screening and / or diagnosing a respiratory, cardiopulmonary disorder or disease such as chronic heart failure, and / or methods for monitoring the efficacy of therapeutics administered to subject to treat the disorder or disease.

The invention discloses a pH and light dual-response target medicine loading system. Medicine molecules in the medicine loading system are stored in mesoporous silicon dioxide microspheres through adsorption, the surfaces of the microspheres are modified by silane coupling agent with aldehyde groups as end groups and ylmethyl azobenzene, and the microspheres are packaged with macrocycle molecule-alpha cyclodextrin. By synthesizing the silane coupling agent containing aldehyde groups and ylmethyl azobenzene and modifying the surfaces of the mesoporous silicon dioxide microspheres with the silane coupling agent and ylmethyl azobenzene, medicine molecules are adsorbed, and the medicine molecules are packaged with cyclodextrin to prepare the target medicine loading system. Compared with other medicine loading systems, the target medicine loading system has the advantages of being large in release amount, high in release speed, easy to adjust and high in sensitivity, synergetic controlled release can be achieved under stimulation of pH and ultraviolet, and dual-response release performance is achieved; by controlling the pH and ultraviolet conditions, controllable release and periodical release of medicine are achieved. Cell experiments prove that the target medicine loading system has good application prospects in the field of biological medicine in the aspect of target medicine loading.

The invention discloses a reductive stimulation response drug carrier containing carbon nano cages. The carbon nano cages (CNC) are used as a core, dendritic macromolecules coat the surface of each CNC, the CNCs are connected through disulfide bonds, the macromolecules are connected through disulfide bonds, and the CNCs and the macromolecules are connected through disulfide bonds; the dendritic macromolecules are polyamide-amide dendritic macromolecules (PAMAM) of low generation G0, G1, G2 and / or G3, and are PAMAM-NH2 with amino groups as terminal groups or PAMAM-COOH with carboxy groups as terminals. The invention further provides a preparation method and application thereof. The nano carrier has high loading capacity and can sensitively release drug under stimulation of a reductant like glutathione, and drug release can be promoted through laser irradiation. Tumor tissue contains high-concentration glutathione, so that drug carrying particles have important medicinal prospect in tumor targeted treatment.

The invention relates to a photo-responsive micro valve based on a micro-fluidic chip and a production method thereof. Microstructures and micro-channels are arranged on the surface of the micro-fluidic chip, a hydrophilic / dehydrophilic gate controlled switch is arranged on a main micro-channel, the surfaces of the micro-channels are modified through photo-responsive molecules, reversible changes of surface appearance and chemical construction of the micro-channels cause changes of surface invasion under stimulation of visible lights, movement of micro-fluid on the surfaces of the micro-channels is controlled, and therefore the intelligent micro valve achieves control on micro-fluid flowing. The photo-responsive micro valve is mainly applied to the related fields such as electrophoretic separation, chromatographic separation and immunoassay, achieves intelligent driving and control on micro-fluid, greatly reduces micro-fluid driving and control cost, has the advantages of being portable, economical, fast and efficient, and is a novel analysis technique for micro-fluid driving and control.

The invention discloses a drug nano preparation and a preparation method thereof. The drug nano preparation comprises a nano carrier. The nano carrier is of a core-shell structure formed by a hydrophilic outer chain and a lyophobic core. The hydrophilic outer chain is a water soluble polymer or water soluble polysaccharide; the lyophobic core is polyamide-amine. siRNA is adsorbed to the surface of the lyophobic core and an anti-tumor drug and a lyophobic probe are buried therein; the hydrophilic outer chain is connected to the lyophobic core through azobenzene-4,4-dicarboxylic acid. The low-oxygen responsive nano carrier is prepared by connecting methoxyl polyoxamide (PEG) and polyamide-amine (PAMAM) through azobenzene-4,4-dicarboxylic acid and can be used for co-carrying chemotherapeutics and siRNA. The nano preparation not only is reduced in grain size and strong in penetrability under stimulation of low-oxygen micro-environment of tumor, but also is simple in preparation process, and the toxicity to tumor cells is increased.

The invention discloses a preparation method of an ultrathin high strength hydrogel membrane. The preparation method comprises the steps of mixing a hydrophilic monomer, a hydrophobic monomer, an initiator and a solvent A to obtain a mixed liquor, and performing free radical polymerization to obtain a random copolymer solution; and dripping the random copolymer solution into a solvent B, spreading on the surface of the solvent B to form an ultrathin high strength hydrogel membrane, wherein the solvent A is a single solvent or a mixed solvent capable of simultaneously dissolving the hydrophilic monomer, the hydrophobic monomer and the random copolymer, the solvent B is a poor solvent of the random copolymer and is a single solvent or a mixed solvent, and the solvent A and solvent B are much different in surface tension. The hydrogel membrane prepared by the method has the thickness of 10-200mu m, has good mechanical property, can be converted into sol and has repairable function under stimulation of pH, temperature and solvent, and can be used in the fields of medical wound dressing, separating membrane materials, facial mask base materials.

The invention discloses a photosensitive dopant, a preparation method thereof, a self-assembly system and a photoresponse device. The chemical structure of molecules of the photosensitive dopant is characterized in that 3,5-bi-(4-hydroxyphenylazo) benzoic acid is adopted as a base framework, a carboxyl terminal is connected with a chirality group, and a hydroxyl terminal is connected with a flexible chain. Under stimulation of external light, a photosensitive group- di-azobenzene with high ultraviolet absorption capability can achieve quick response and can be isomerized, quick isomerization of the chirality group in the structure and even the entire molecules is further induced, and the deformation index of the molecules is higher and quicker. In the self-assembly system of the molecules, rearrangement of the molecules in the system can be triggered more obviously, and physicochemical characteristics, like optimal and electrical characteristics, of the system are regulated.

The invention discloses a water-soluble terbium-doped calcium fluoride nanoparticle and a preparation method thereof. The preparation method comprises the following steps: mixing 2,6-pyridinedicarboxylic acid, NaOH, inorganic calcium salt, inorganic terbium salt and alkali metal fluoride to prepare reaction mixed liquor; performing hydrothermal reaction on the reaction mixed liquor to prepare a colloidal solution; purifying the colloidal solution to obtain the water-soluble terbium-doped calcium fluoride nanoparticle. The invention further provides application of the water-soluble terbium-doped calcium fluoride nanoparticle prepared by the preparation method in transition metal ion recognition. The prepared nanoparticle disclosed by the invention has the advantages of even size distribution, small particle diameter, ability in emitting strong Tb<3+> character fluorescence under stimulation of ultraviolet light and recognizing effect with transition metal ions. In addition, the water-soluble terbium-doped calcium fluoride nanoparticle disclosed by the invention has the advantages of simple preparation method, easiness in being amplified, good controllability, excellent product performance, ability in recognizing metal ions and hope in being applied to the fields of analytical chemistry, biological and medical fluorescent probes, tracing, counterfeit prevention and the like.

Substrates for cell culture and tissue engineering bioreactors consisting of polymers that change their shape over time under stimulation by temperature change, hydration, degradation, or other means. A method of controlling cell culture using a biodegradable shape memory polymer, wherein shape changes can transfer stresses, strains, or both to adherent or otherwise connected cells such that the mechanical stimulus impacts cell development and the resulting properties of tissues.

The invention relates to a nervous network restoration system and a preparation method thereof. The nervous network restoration system comprises a sustained-release diaphragm, a photosensitive astrocyte adsorbed on the sustained-release diaphragm, and a positively charged conducting polymer dispersed in the sustained-release diaphragm, wherein the sustained-release diaphragm is a hydrogel diaphragm formed by virtue of crosslinking of polyacrylic acid and polyvinyl alcohol. When the nervous network restoration system is used for treating an injured nervous network, the photosensitive astrocyte can specifically increase ATP (Adenosine Triphosphate) release under stimulation of light irradiation, negatively charged ATP is attracted by the positively charged conducting polymer to deposit on the surface of the sustained-release diaphragm, so that release of ATP is realized by diffusion, the defect of transient release of conventional drug treatment is overcome, stem cell proliferation and neuron directional differentiation can be promoted well, and the injured nervous network can be restored effectively.

The invention discloses a diazosulfide benzyl cyanide derivative and a preparation method and application thereof. The preparation method for the derivative comprises the following steps that triphenylamine boric acid, diazosulfide and a benzyl cyanide derivative are taken as raw materials and are subjected to Suzuki coupling and Knoevenagel condensation reactions successively to synthesize the target product diazosulfide benzyl cyanide derivative, namely a force-induced fluorescence enhancement material. The color of the material changes under stimulation of lower pressure (the Mpa level), and the material has the advantages that the contrast ratio is high, and fluorescence intensity is improved significantly (the quantum efficiency is improved to 19.3% from 9%), and can be applied to a pressure sensing system.

The invention relates to a method for the preparation of efficient antigen-presenting human γδ T cells, to the γδ T cells prepared by such a method, and to their use in immunotherapy, vaccination, vaccine development and diagnostics. Similar to dendritic cells (DCs) in potency and efficacy, these human γδ T cells process antigens and present antigenic peptides to αβ T cells and induce antigen-specific responses (proliferation and differentiation) in naïve αβ T cells. γδ T cells are easily purified from peripheral blood, acquire “maturation” status (expression of essential adhesion, co-stimulatory and major histocompatibility complex molecules) within 1 day of in vitro culture under stimulation and induce strong primary and secondary T helper cell responses. The γδ T cells may be used in a method of treatment of tumors or chronic or recurrent infectious diseases, in identification of novel tumor or pathogen-derived antigens, and in the diagnosis of the immune competence of a patient.

The invention provides a nitrogen-doped carbon dot based on waste tobacco stems, a synthetic method of the carbon dot. The synthetic method includes the steps: taking the waste tobacco stems as a carbon source; taking water as a solvent; performing hydrothermal reaction on the waste tobacco stems and the water in a reaction kettle; performing filtering, dialysis, concentration and drying after reaction is completed to prepare the nitrogen-doped carbon dot. The surface of the nitrogen-doped carbon dot is rich in functional groups such as carboxyl and amidogen, average particle diameter is about4.7nm, water solubility is good, and the carbon dot can emit bright fluorescence under stimulation of ultraviolet light, can stimulate dependent fluorescence emission behaviors and can serve as a fluorescent probe applied to the aspects of biological imaging and the like.

The invention relates to a magnetic responsive micro valve based on a micro-fluidic chip and a production method thereof. Microstructures and micro-channels are arranged on the surface of the micro-fluidic chip, a hydrophilic / dehydrophilic gate controlled switch is arranged on a main micro-channel, the surfaces of the micro-channels are modified through magnetic responsive molecules, reversible changes of surface appearance and chemical construction of the micro-channels cause changes of surface invasion under stimulation of an outer magnetic field, movement of micro-fluid on the surfaces of the micro-channels is controlled, and therefore the intelligent micro valve achieves control on micro-fluid flowing. The magnetic responsive micro valve is mainly applied to the related fields such as electrophoretic separation, chromatographic separation and immunoassay, achieves intelligent driving and control on micro-fluid, greatly reduces micro-fluid driving and control cost, has the advantages of being portable, economical, fast and efficient, and is a novel analysis technique for micro-fluid driving and control.

The invention relates to an intelligent micro valve based on a micro-fluidic chip and a production method thereof. Microstructures and micro-channels are arranged on the surface of the micro-fluidic chip, a hydrophilic / dehydrophilic gate controlled switch is arranged on a main micro-channel, the surfaces of the micro-channels are modified through thermo-responsive molecules, reversible changes of surface appearance and chemical construction of the micro-channels cause changes of surface invasion under stimulation of external field temperature, movement of micro-fluid on the surfaces of the micro-channels is controlled, and therefore the intelligent micro valve achieves control on micro-fluid flowing. The intelligent micro valve is mainly applied to the related fields such as electrophoretic separation, chromatographic separation and immunoassay, achieves intelligent driving and control on micro-fluid, greatly reduces micro-fluid driving and control cost, has the advantages of being portable, economical, fast and efficient, and is a novel analysis technique for micro-fluid driving and control.