Preparation of antigen-presenting human gamma delta t cells and use in immunotherapy

A cell and antigen technology, which is applied to the preparation of antigen-presenting human γδT cells and its application in immunotherapy, and can solve the problems of demanding technology, high cost, and unstable function.

Inactive Publication Date: 2007-09-05
UNIV COLLEGE CARDIFF CONSULTANTS LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, DCs are functionally unstable and undergo a predetermined sequence of irreversible differentiation steps, resulting in impaired ("depleted") immune function
This poses great difficulties in generating functionally similar DC preparations by in vitro manipulation
Finally, the generation of peptide-presenting DCs for use in immunotherapy is technically demanding, time-consuming, and costly

Method used

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  • Preparation of antigen-presenting human gamma delta t cells and use in immunotherapy
  • Preparation of antigen-presenting human gamma delta t cells and use in immunotherapy
  • Preparation of antigen-presenting human gamma delta t cells and use in immunotherapy

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Experimental program
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Embodiment

[0123] abbreviation

[0124] DC dendritic cells

[0125] LN lymph nodes

[0126] PP Peyer's junction

[0127] TCR T cell antigen receptor

[0128] BCR B cell antigen receptor

[0129] MHC major histocompatibility complex

[0130] APC antigen presenting cells

[0131] Vδ1 + T cells express Vδ1 + - TCR chain of γδ T cells

[0132] Vγ2Vδ2 + T cells express Vγ2Vδ2 + - TCR chain of γδ T cells

[0133] IPP isopentenyl pyrophosphate

[0134] TT Clostridium tetani tetanus toxin

[0135] PPD Purified protein derivative of Mycobacterium tuberculosis

[0136] TSST-1 Staphylococcus aureus toxic shock syndrome toxin 1

[0137] PHA Phytohemagglutinin

[0138] IFN-γ Interferon-γ

[0139] TNF-α Tumor Necrosis Factor-α

[0140] IL interleukin

[0141]FACS Fluorescence Activated Cell Sorting

[0142] MFI Mean Fluorescence Intensity

[0143] SD standard error

[0144] CFSE Carboxyfluorescein diacetate succinimidyl ester

[0145] 1. Cell Isolation and Generation

[0146...

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Abstract

The invention relates to a method for the preparation of efficient antigen-presenting human Gamma Delta T cells, to the Gamma Delta T cells prepared by such a method, and to their use in immunotherapy, vaccination, vaccine development and diagnostics. Similar to dendritic cells (DCs) in potency and efficacy, these human Gamma Delta T cells process antigens and present antigenic peptides to Alpha Beta T cells and induce antigen-specific responses (proliferation and differentiation) in nave Alpha Beta T cells. Gamma Delta T cells are easily purified from peripheral blood, acquire''maturation'' status (expression of essential adhesion, co-stimulatory and major histocompatibility complex molecules) within 1 day of in vitro culture under stimulation and induce strong primary and secondary T helper cell and cytotoxic T cell responses. The Gamma Delta T cells may be used in a method of treatment of tumors or chronic or recurrent infectious diseases, in identification of novel tumor or pathogen-derived antigens, and in the diagnosis of the immune competence of a patient.

Description

technical field [0001] The present invention relates to a method for preparing human γδT cells that can effectively present antigens, the γδT cells prepared by the method and their use in immunotherapy, antigen identification and immunity diagnosis. Background technique [0002] Cellular components of the adaptive immune system [0003] The cellular components of the immune system are divided into cells of the innate immune system and cells of the adaptive (acquired or specific) immune system. Cells of the innate immune system include, inter alia, monocytes, granulocytes, natural killer cells in the peripheral blood, and mast cells, macrophages, and dendritic cells (DCs) in the extravascular compartment, which Includes peripheral tissues such as skin, airways, gastrointestinal and genitourinary tracts, internal organs, and secondary lymphoid tissues such as spleen, lymph nodes (LN) and Peyer's patches (PP). The main functions of innate cells are a) immediate protection by ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/08
Inventor B·莫瑟M·布兰德斯
Owner UNIV COLLEGE CARDIFF CONSULTANTS LTD
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