1936results about "Tissue culture" patented technology

A system for perfusing an organ, for example a kidney or a heart. Preservation fluid can be pumped from a transport container to the organ through a cannula or organ adapter. A flow regulator valve can control the rate of flow of the preservation fluid based on renal resistance of the kidney or arterial resistance of the organ. The pump can operate at a rate independent of the arterial resistance.

A transport container for transporting an organ (T), such as a kidney, at hypothermic temperatures. The transport container comprises an inner lid (40) and a canister (38) that can be filled with preservation fluid. The preservation fluid can be pumped from the canister to the organ through a cannula. A flow regulator valve (48) can release the preservation fluid from the fluid path into the transport container at a rate based on at least one organ parameter (such as renal resistance of the kidney), whereas the pump can operate at a rate independent of the at least one organ parameter. The transport container may further comprise a fill port (28) and a vent port in the inner lid, and an organ rest (18).

A method for transporting a biological sample at hypothermic temperatures. The container can be cooled using phase change material and pump fluid through the sample. The fluid can pump through the system at a rate independent of the parameters of the biological sample. A valve can control the rate of flow of the fluid into the biological sample.

The invention relates to an HPV16 type E6 oncoprotein monoclonal antibody as well as a preparation method and application thereof. The monoclonal antibody specifically bound with the HPV16E6 recombinant antigen is obtained through screening, the monoclonal antibody is applied to a biotin-avidin amplification ELISA system, the E6 oncoprotein of HPV16 can be specifically detected, the sensitivity is 100 pg / mL, the monoclonal antibody does not intersect with oncoprotein of other high-risk HPV, and the E6 oncoprotein of HPV16 can be specifically detected. High specificity and sensitivity are shown for cervical exfoliated cell sample detection of normal people, CIN3 people and cervical cancer people, and the method can be applied to early screening of cervical cancer in the future; compared with traditional exfoliative cytology examination, the method is faster and more accurate, meanwhile, the risk of false positive and misdiagnosis caused by too high sensitivity of nucleic acid detection can be avoided, and the method has very high application value.

Systems and methods are provided for stabilization of cells and retention of biomolecules therein for further analysis. Block copolymers are inserted into membranes of biological particles, cell membranes or organelles (e.g., organelle membranes) and polymerized to form an intra-biological particle (e.g., intracellular) polymer network.

A method for transporting a biological sample at hypothermic temperatures. The container can be cooled using phase change material and pump fluid through the sample. The fluid can pump through the system at a rate independent of the parameters of the biological sample. A valve can control the rate of flow of the fluid into the biological sample.

The present application discloses a cell culture unit, device, application and culture method, wherein the cell culture unit includes a substrate, a culture chamber, a liquid storage chamber and a flow channel chamber are formed in the substrate, and the culture chamber and the liquid storage chamber have an opening at the top of the substrate, and the two ends of the flow channel chamber are connected to the culture chamber and the liquid storage chamber respectively, and the flow channel chamber includes a first port portion connected to the liquid storage chamber and a second port portion connected to the culture chamber, and the flow channel chamber is also formed with a constriction portion between the first port portion and the second port portion, and the opening area of ​​the constriction portion is smaller than the opening area of ​​the first port portion and the second port portion. The technical solution of the present application can avoid the impact on the biological sample caused by too fast flow rate, resulting in the cells in the liquid gel being dispersed by the culture medium, and increase the time for the culture medium to infiltrate the biological sample to fully infiltrate the biological sample, thereby being able to improve the simulation degree of the cell model finally obtained and improve the success rate of culture.

The invention relates to the technical field of biomedicine, in particular to an anti-influenza A virus NP protein antibody pair and application thereof. The antibody pair comprises two monoclonal antibodies; the monoclonal antibody comprises a heavy chain variable region and a light chain variable region; one monoclonal antibody in the monoclonal antibody pair is named as a 10G10 antibody, and the other monoclonal antibody is named as a 11F8 antibody. The invention also discloses a nucleic acid molecule for coding the monoclonal antibody and a hybridoma cell strain for secreting the monoclonal antibody. In addition, the invention also discloses a kit for detecting influenza virus protein, which comprises the monoclonal antibody pair, the 10G10 antibody is used as a capture antibody, and the 11F8 antibody is used as a labeled antibody. The antibody for resisting the influenza A virus NP protein provided by the invention has relatively good specificity on the influenza virus protein.

Provided are an apolipoprotein E detection reagent and the use thereof. The reagent comprises at least one of an ApoE2 protein detection reagent, an ApoE3 protein detection reagent and an ApoE4 protein detection reagent. One of a capture antibody and a detection antibody used for detecting ApoE2 protein or ApoE4 protein is a specific monoclonal antibody, and the capture antibody and the detection antibody used for detecting ApoE3 are both specific monoclonal antibodies. Genotyping of ApoE and quantitative detection of different genotypes of proteins are performed on the basis of an immunological detection method. The detection method has the advantages of being simple, easy to operate, short in time and cheap. A genotype detection result is highly consistent with a fluorescence PCR result. Genotyping of ApoE (6 types) can be performed, which is used to replace the nucleic acid detection and guide medication, and clarify the correlation of the ApoE4 genotype homozygosity / heterozygosity and the protein concentration of ApoE4 with AD.

The invention discloses monoclonal antibodies NP5 and NP8 for resisting influenza A virus NP protein and application of the monoclonal antibodies NP5 and NP8, and belongs to the technical field of monoclonal antibodies. According to the invention, 19 influenza A virus NP protein monoclonal antibodies are prepared, and the monoclonal antibodies NP5 and NP8 are obtained by screening through ELISA (enzyme-linked immuno sorbent assay) detection and sequencing analysis. The NP5 comprises a light chain CDR2 of which the amino acid sequence is RTS, light chains CDR1 and CDR3 of which the amino acid sequences are shown as SEQ ID NO.3-4, and heavy chains CDR1-3 of which the amino acid sequences are shown as SEQ ID NO.7-9; the NP8 comprises a light chain CDR2 of which the amino acid sequence is LVS, light chains CDR1 and CDR3 of which the amino acid sequences are as shown in SEQ ID NO.12 to SEQ ID NO.13, and heavy chains CDR1-3 of which the amino acid sequences are as shown in SEQ ID NO.16 to SEQ ID NO.18. The invention lays a foundation for preparing an influenza A virus detection kit.

The invention belongs to the field of monoclonal antibodies, and particularly relates to a mouse anti-pelteobagrus fulvidraco IgM monoclonal antibody, a hybridoma cell strain secreting the monoclonal antibody and application, a CDR region of a heavy chain variable region of the mouse anti-pelteobagrus fulvidraco IgM monoclonal antibody comprises three sequences shown in SEQ ID NO.1-3, and a CDR region of a light chain variable region of the mouse anti-pelteobagrus fulvidraco IgM monoclonal antibody comprises three sequences shown in SEQ ID NO.4-6; the mouse anti-pelteobagrus fulvidraco IgM monoclonal antibody can be obtained by being secreted by a hybridoma cell strain PFM-1B7C12A1B2 with the preservation number of CCTCC (China Center for Type Culture Collection) NO: C2024234. The mouse anti-pelteobagrus fulvidraco IgM monoclonal antibody provided by the invention can be used for Western blot and ELISA specific detection of IgM in a pelteobagrus fulvidraco secretion form, and can be used for flow cytometry identification of pelteobagrus fulvidraco IgM + B cells. The monoclonal antibody is good in specificity, high in sensitivity and strong in affinity, can be used for structural analysis, immune response level detection and the like of pelteobagrus fulvidraco IgM, and lays a foundation for in-depth study of a pelteobagrus fulvidraco immune system and establishment of a vaccine immune effect evaluation method taking the antibody level as an index.

The invention relates to the technical field of cell preparation quality evaluation, in particular to a cell preparation whole-process quality intelligent evaluation system and a method thereof, and the system comprises a data acquisition module, a preprocessing module, a mining module, a modeling module, a report automatic generation module and a data storage module. The modeling module is used for constructing a cell product index evaluation system and a product traceability model and comprises a cell culture state evaluation model and an environment monitoring analysis module, and the environment monitoring analysis module is used for carrying out traceability analysis by using a time sequence convolutional neural network; the automatic report generation module intelligently evaluates the quality of the whole process and generates a report, the data storage module manages data and model storage, sharing and calling, the system constructs a cell quality evaluation system based on multi-dimensional data, integrates multiple dimensional indexes such as morphology, surface markers and functionality, realizes comprehensive evaluation, and improves the evaluation efficiency. And the one-sidedness of single index evaluation is avoided.

The invention provides an FWCH2 monoclonal antibody and a preparation method and application of a kit of the FWCH2 monoclonal antibody. A monoclonal antibody hybridoma cell strain FWCH2 is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.46112, and the prepared monoclonal antibody can effectively recognize the FWCH2.

The invention discloses a hybridoma cell strain, a monoclonal antibody, a linear epitope antigen and application thereof, and relates to the field of genetic engineering, in particular to a hybridoma cell strain, a monoclonal antibody, a linear epitope antigen and application thereof. The preservation number of the hybridoma cell strain P27-A1 is CGMCC (China General Microbiological Culture Collection Center) No. 46352. The hybridoma cell strain P27-A1 is prepared from feline leukemia virus p27, and a monoclonal antibody secreted by the hybridoma cell strain P27-A1 recognizes a linear epitope antigen of feline leukemia virus p27 protein; the kit for the feline leukemia virus p27 comprises the monoclonal antibody A1 secreted by the hybridoma cell strain P27-A1. The monoclonal antibody secreted by the hybridoma cell strain can greatly improve the sensitivity of the p27 protein for detecting feline leukemia virus. The invention provides the monoclonal antibody secreted by the hybridoma cell strain for identification, and the monoclonal antibody can be used for preparing a diagnostic kit such as a colloidal gold test strip.

The invention belongs to the field of veterinary biotechnology detection, and particularly relates to a hybridoma cell strain and a monoclonal antibody for detecting or identifying MDV-1 and application of the hybridoma cell strain and the monoclonal antibody. The hybridoma cell strain MDV-1Mab-pp38-E2 is obtained through screening by taking pp38 protein of MDV-1 as immunogen, the preservation number is CCTCC NO: C202501, the monoclonal antibody generated by the hybridoma cell strain can recognize I-type chicken Marek's disease virus strains with different serum, and the monoclonal antibody can be used for identifying the type I chicken Marek's disease virus strains with different serum types. The kit has good specificity and sensitivity and can be used for exogenous virus detection of the serum type I chicken Marek's disease virus in poultry virus live vaccines without cross reaction with serum type II chicken Marek's disease virus, serum type III chicken Marek's disease virus, chicken egg drop syndrome virus (EDSV) and other common poultry disease viruses, and the kit has good specificity and sensitivity and can be applied to exogenous virus detection of the serum type I chicken Marek's disease virus in poultry virus live vaccines. The kit can also be used for clinical identification, virus content determination and epidemiological investigation of serotype I chicken Marek's disease virus.

The invention provides a K1143 monoclonal antibody and a preparation method and application of a kit of the K1143 monoclonal antibody. The monoclonal antibody hybridoma cell strain K1143 is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.46110, and the prepared monoclonal antibody can effectively recognize K1143. The invention further discloses a preparation method of the monoclonal antibody K1143.

The invention provides an LRC58 monoclonal antibody and a preparation method and application of a kit of the LRC58 monoclonal antibody. The monoclonal antibody hybridoma cell strain LRC58 is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.46113, and the prepared monoclonal antibody can effectively recognize the LRC58. The invention further discloses a method for preparing the monoclonal antibody.

The invention belongs to the technical field of biological detection, and relates to an antibody composition aiming at pTau217 protein detection and application thereof, and the antibody composition aiming at pTau217 protein detection comprises a target pTau217 protein antibody and a target Tau protein C-terminal antibody. Meanwhile, based on the antibody composition aiming at pTau217 protein detection, the invention further provides a kit for early diagnosis of the Alzheimer's disease. The invention provides an antibody composition aiming at pTau217 protein detection, which is stronger in detection specificity and higher in sensitivity, and an application of the antibody composition.

The invention provides an SCGB1D4 monoclonal antibody and a preparation method and application of a kit of the SCGB1D4 monoclonal antibody. An applicant is preserved in the China General Microbiological Culture Collection Center (CGMCC) through a hybridoma cell strain SCGB1D4, and the preservation number is CGMCC No.45958. The prepared monoclonal antibody can effectively recognize the SCGB1D4.

The invention relates to the technical field of biology, and aims to provide a monoclonal antibody 7A12 for identifying gene type 2 swine fever as well as a preparation method and an application method of the monoclonal antibody 7A12. The monoclonal antibody comprises Ig structural domains VH CDR1, VH CDR2 and VH CDR3 of a heavy chain of the antibody, and Ig structural domains VL CDR1, VL CDR2 and VL CDR3 of a light chain of the antibody, wherein the amino acid sequences of the VH CDR1, the VH CDR2 and the VH CDR3 are respectively as shown in SEQ ID NO: 1 to SEQ ID NO: 3; the amino acid sequences of the VL CDR1, the VL CDR2 and the VL CDR3 are respectively as shown in SEQ ID NO: 4 to SEQ ID NO: 6. The antibody provided by the invention has the advantages of high titer, strong specificity, high affinity and the like; the kit has the advantages of high sensitivity and strong specificity, has no cross reaction with the type 1 swine fever vaccine strain, and can identify and diagnose swine fever gene type 2 wild strain infection and vaccine immunity in serology; the method can be further used for establishing a blocking ELISA kit for identifying the gene type 2 hog cholera, and can effectively diagnose and purify the gene type 2 hog cholera virus.

The invention belongs to the field of biology, and discloses a monoclonal antibody of avian influenza virus PB2 protein, the monoclonal antibody comprises a heavy chain variable region and a light chain variable region, and can specifically bind to influenza virus matrix protein PB2, and the amino acid sequence of the heavy chain variable region is as shown in SEQ ID NO.1; the amino acid sequence of the light chain variable region is as shown in SEQ ID NO. 2; the hybridoma cell has relatively high sensitivity and specificity, and can be specifically combined with at least five subtype avian influenza PB2 proteins; meanwhile, the invention also provides application and drugs of the labeled compound, the hybridoma cell and the monoclonal antibody.

An object of the present invention is to provide a method for preserving a platelet in a refrigerated or frozen state while retaining more platelet functions, a platelet preservation solution for use in such a method, and the like. The present invention relates to a platelet preservation solution for refrigeration or freezing, comprising an electrolyte and an anticoagulant. The present invention also relates to a method for preserving a platelet, comprising: suspending a platelet in the platelet preservation solution according to the present invention; and preserving the suspension by refrigeration or freezing at 10°C or lower.

The invention is applicable to the technical field of C-reactive protein detection, and provides an anti-C-reactive protein monoclonal antibody idiotype antibody, a preparation method and application, and the anti-C-reactive protein monoclonal antibody idiotype antibody is secreted by a hybridoma cell strain; the hybridoma cell strain is a 7C4 hybridoma cell strain; the preparation method of the anti-C-reactive protein monoclonal antibody idiotype antibody comprises the following steps: S1, obtaining hybridoma cells: preparing an anti-C-reactive protein monoclonal antibody Fab fragment, then carrying out animal immunization, then carrying out cell fusion, and finally screening and cloning the hybridoma cells; and S2, preparing and screening an anti-C reactive protein monoclonal antibody idiotype antibody. The anti-C-reactive-protein monoclonal antibody idiotype antibody is used for replacing C-reactive protein to serve as a labeled antibody, the labeling process is simple, the labeling efficiency is high, and meanwhile the situation that a final product is affected due to instability of the C-reactive protein is avoided. Experimental results show that the detection method is good in specificity and wide in linear range.

The invention discloses an anti-HCV (hepatitis C virus) core antigen monoclonal antibody, a hybridoma cell strain and application, and particularly relates to the hybridoma cell strain screened according to a common conserved sequence of core proteins of different genotypes of HCV, the antibody secreted by the hybridoma cell strain is applied to HCV antigen detection, different genotypes of hepatitis C viruses can be detected, and the monoclonal antibody has good inclusiveness and can be applied to detection of HCV antigens. The technical effect can be realized by only two antibodies, so that the production cost of the HCV antigen detection kit can be reduced, the production difficulty can be reduced, and the production efficiency can be improved.

Provided herein is a bioengineering and machine learning framework for complex tissue development, which can be implemented for the identification and / or assessment of gene and / or drug therapies.

The present invention provides a monkey procalcitonin recombinant protein, a monoclonal antibody and a preparation method and application thereof, wherein the amino acid sequence of the monkey procalcitonin recombinant protein is shown in SEQ ID No. 1, and the nucleotide sequence encoding the recombinant protein is shown in SEQ ID No. 2. By optimizing the antigen peptide sequence and the expression vector, the efficient and soluble expression of monkey procalcitonin in Escherichia coli is achieved, and a mouse monoclonal antibody specifically binding to monkey procalcitonin is screened based on the monkey procalcitonin recombinant protein, and the above mouse monoclonal antibody is used to screen a pairable monoclonal antibody, and further based on the monoclonal antibody combination, a double antibody sandwich immune ELISA detection method or kit applied to monkey procalcitonin recombinant protein is obtained, which has high detection sensitivity and can be well applied to the detection of monkey serum.

A deaminase, a fusion protein and the use thereof. The deaminase contains an amino acid sequence having at least 50% sequence identity to any one of SEQ ID NOs: 1-6, 39-164 and 297-312, and the deaminase can deaminize at least one nucleotide in a target polynucleotide. The deaminase can be fused with a nucleic acid-binding polypeptide to obtain various base editors for the sequence-specific base conversion of the target polynucleotide. The performance of the editors can be further improved by means of fusing one or two UGIs, fusing a Gam protein and other strategies.

This disclosure provides nanoparticle compositions, and use thereof for isolating and measuring proteins, protein degrader action, and cells. The disclosed nanoparticles can be identified by barcodes that reveal the identity of and post translational modifications to the bound protein using image processing techniques described herein.