1103 results about "Tyrosinase" patented technology

The present invention includes the receptor protein 4-1BB and the cDNA gene encoding for receptor protein 4-1BB. The nucleotide sequence of the isolated cDNA is disclosed herein along with the deduced amino acid sequence. The 4-1BB protein and fragments and derivatives can be used: 1) as a probe to isolate ligands to receptor protein 4-1BB, 2) to stimulate proliferation of B-cell's expressing 4-1BB, or 3) to block 4-1BB ligand binding. A monoclonal antibody against 4-1BB was developed which specifically recognizes an epitope on the extracellular domain of receptor protein 4-1BB. The monoclonal antibody can be used enhance T-cell proliferation and activation by treating T-cells that have expressed receptor protein 4-1BB with the monoclonal antibody. The effectiveness of the treatment was enhanced when conducted in the presence of protein tyrosinase kinase. A fusion protein for detecting cell membrane ligands to receptor protein 4-1BB was developed. It comprises the extracellular portion of the receptor protein 4-1BB and a detection protein bound to the portion of the receptor protein 4-1BB.

A method of manufacturing a biopolymer optical device includes providing a polymer, providing a substrate, casting the polymer on the substrate, and enzymatically polymerizing an organic compound to generate a conducting polymer between the provided polymer and the substrate. The polymer may be a biopolymer such as silk and may be modified using organic compounds such as tyrosines to provide a molecular-level interface between the provided bulk biopolymer of the biopolymer optical device and a substrate or other conducting layer via a tyrosine-enzyme polymerization. The enzymatically polymerizing may include catalyzing the organic compound with peroxidase enzyme reactions. The result is a carbon-carbon conjugated backbone that provides polymeric “wires” for use in polymer and biopolymer optical devices. An all organic biopolymer electroactive material is thereby provided that provides optical functions and features.

Compositions and methods are taught for directing the orientation of an immobilized capture biomolecule on a hydrophobic membrane. The method comprises layering at least one tie layer on a hydrophobic membrane, adding an amine functional layer on top of at least one tie layer; and attaching an alignment biomolecule to the amine functional layer. The alignment biomolecule has the ability to either capture a target biomolecule itself and thus be considered a capture biomolecule, or bind and orient the immobilized capture biomolecule so as to maximize the binding activity of the immobilized capture biomolecule. In one embodiment, a nickel-coordinated amine functional layer binds with a histidine-tagged alignment biomolecule. In another embodiment, an amine functional layer reacts, via tyrosinase catalysis, with a tyrosine residue in an alignment biomolecule.

This invention relates to certain sugar esters of phytosterols of formula (I). These esters are useful for topical application, and for the treatment of skin condition, including age spots, acne, loss of cellular antioxidants, collagen loss, loss of skin pliability, loss of skin suppleness, skin wrinkles including fine lines, oxidation, damage from radiation, malfunction of matrix metalloproteases, malfunction of tyrosinases, damage from free radicals, damage from UV, dry skin, xerosis, ichthyosis, dandruff, brownish spots, keratoses, melasma, lentigines, liver spots, pigmented spots, dark circles under the eyes, skin pigmentation including darkened skin, blemishes, oily skin, warts, eczema, pruritic skin, psoriasis, inflammatory dermatoses, topical inflammation, disturbed keratinization, skin changes associated with aging, nail or skin requiring cleansers, conditioning or treatment, and hair or scalp requiring shampooing or conditioning, and combinations thereof;Wherein,n=0, 1, 2, or 3; andR=H, —CH2OH, —CH(OH)—CH2OH, —CH(OH)—CH(OH)—CH2OH; andR1=Substituent selected from a sapogenin, steroid, or terpenoid.

The invention discloses a whitening composition and a skin care product with the same. The whitening composition and the skin care product contain glycyrrhiza glabra extracts, arbutin and 3-o-ethyl ascorbic acid, and preferably contain nonapeptide-1, nicotinamide, ethylhexyl methoxycinnamate or composition of the nonapeptide-1, the nicotinamide and the ethylhexyl methoxycinnamate. Through mechanism analysis, the composition has the advantages that possibly due to the mutual action and influence among various ingredients, the generation of melanin is inhibited through inhibiting the activity of tyrosinase; the migration of melanocytes to the skin is retarded; in addition, the plant and microbial peptide sourced whitening ingredients and various antiallergic ingredients are selected to be combined to obtain the safe and mild whitening effect.

This application is a continuation-in-part of pending U.S. patent application Ser. No. 10 / 065,995 by the authors, filed on Dec. 9, 2002, for a Commercial Process for Isolation and Purification of Glabridin with High Tyrosinase Inhibitory Activity and its Cosmetic Compositions and Methods Of Use. The current invention discloses the use of Glabridin containing Licorice extract (4-90% Glabridin) as metalloprotease and hyaluronidase inhibiting component in cosmetic topical or oral formulations. These extracts and particularly Glabridin, are useful in anti-wrinkle and anti-aging products, providing elasticity, firmness, tone and texture to the skin, ameliorating fine lines and crows feet in under eye preparations, and prevent skin and hair damage due to UV rays, inflammation and itch, diaper rashes in baby products, as massage or toning oils or emulsion for babies.

Novel pro-drugs and assay reagents are provided which are useful as therapeutic agents especially for delivery and targetting therapeutically active agents to melanoma cells. The pro-drugs and assay reagents are substrates for tyrosinase and may be represented by the formula TyrX-B-ThrAg*. The compounds of formula TyrX-B-ThrAg* are capable of releasing a therapeutically active agent or assayable substance (ThrAg) at a desired location. TyrX- being a residue of an optionally substituted tyrosine analogue.

Compositions and methods are taught for directing the orientation of an immobilized capture biomolecule on a hydrophobic membrane. The method comprises layering at least one tie layer on a hydrophobic membrane, adding an amine functional layer on top of at least one tie layer; and attaching an alignment biomolecule to the amine functional layer. The alignment biomolecule has the ability to either capture a target biomolecule itself and thus be considered a capture biomolecule, or bind and orient the immobilized capture biomolecule so as to maximize the binding activity of the immobilized capture biomolecule. In one embodiment, a nickel-coordinated amine functional layer binds with a histidine-tagged alignment biomolecule. In another embodiment, an amine functional layer reacts, via tyrosinase catalysis, with a tyrosine residue in an alignment biomolecule.

There is a topical anti-aging composition which is configured to interfere with the production of tyrosinase, as well as melanin by reacting with melanocytes as well as reacting with, or acting on keratinocytes. The anti-aging composition comprises an emulsion comprising a plurality of active ingredients comprising a plurality of pigmentation reducing elements which are configured to interrupt or prevent the production of tyrosinase and melanin. In addition there are a plurality of antioxidants a plurality of anti wrinkling agents a plurality of anti-inflammatory agents at least one additional agent comprising at least one of an emollient, a proactant and a conditioning agent.

The present invention relates to a method for detecting phenol compounds in a water body environment through an enzyme electrochemical biosensor. The biological sensor detection apparatus comprises a working electrode, an auxiliary electrode, a reference electrode, a detection cell and an electrochemical workstation. The specific operation steps comprise: adding a hydrophilic ionic liquid (such as 1-butyl-3-methylimidazolalanine and BMIM [Ala]) to a mesoporous carbon solution, and shaking for half an hour to obtain a novel mesoporous carbon and hydrophilic ionic liquid composite material; adding tyrosinase to the mesoporous carbon and hydrophilic ionic liquid composite material solution, shaking for one hour, and adding the obtained solution to the surface of a working electrode in a dropwise manner to obtain a tyrosinase immobilized working electrode; and inserting the tyrosinase immobilized working electrode, the auxiliary electrode and the reference electrode into a detection cell filled with a phosphate buffer solution, and adding a single standard substance of a phenol compound under a stirring condition to produce a corresponding current response signal.

The invention relates to a water-soluble active extract derived from fruit peels, the preparation method and the application thereof. The fruit peel is treated by the steps of drying, pulverization, extraction, flocculation, separation and concentration, to prepare a water-soluble active extract. Then, the extract is added in cosmetic matrixes to allow a cosmetic to have skin-whitening and anti-skin ageing functions. The fruit peel extract is obtained after the screening of tyrosinase-inhibiting, antioxidant and free radical-scavenging activities in the water-soluble extracts of fruit peels such as Citrus peels, and has skin-whitening and anti-skin ageing effects. The water-soluble active extract has the advantages of easily-accessible raw materials, renewable resources and simple preparation method. The obtained extract has good stability and durable effect and serves as cosmetic additives in any stage of cosmetic production, without the risks of solvent residue and microbial contamination.

The invention discloses a nano fiber whitening and freckle-removing mask paste. The nano fiber whitening and freckle-removing mask paste comprises a spinning solution and cotton cloth, wherein active components of the spinning solution are reduced glutathione and an anti-oxidizing auxiliary material; the mass ratio of the reduced glutathione to the anti-oxidizing auxiliary material is equal to 1-10. According to the nano fiber whitening and freckle-removing mask paste, whitening and freckle-removing effective components are the reduced glutathione (GSH) and the anti-oxidizing auxiliary material, wherein the GSH is dispersed into a nano fiber in a nano particle manner; the anti-oxidizing auxiliary material can keep a reduced state of an active radical sulfhydryl (-SH) in the GSH and the biological activity is kept. The reduced glutathione inhibits the activity of tyrosinase, eliminates active oxygen or free radicals, treats radiation diseases and carries out radiation protection, so as to inhibit the formation of melanin; the reduced glutathione is combined with an electrostatic spinning technique to realize advantage complementation.

The invention discloses a whitening composition and cream thereof. The whitening cream contains a whitening composition which is prepared from nicotinamide, arbutin, citrus peel extractives, tea extractives, scutellaria root extractives, cucumber extractives, apple extractives and the like, wherein the nicotinamide can enhance the skin barrier function, and has the effects of whitening the skin and resisting aging and acnes; the arbutin can inhibit activity of tyrosinase to achieve the whitening effect, and can protect the skin against free radical damage; the citrus peel extractives can enhance the skin brightness, lower percutaneous water loss and enhance the skin moisturizing and barrier functions; due to the synergistic effect of the tea extractives, the scutellaria root extractives, the cucumber extractives and the apple extractives, the effects of whitening, skin tendering and moisturizing are enhanced; the whitening composition is used in whitening skin care products such as a whitening cream product, dark skin and primary spots on the skin can be remarkably improved, the deposited stains and coloring matter accumulated over a long period can be faded and relieved, and the whitening effect is obvious.

The invention discloses a compound traditional Chinese medicine extract and application thereof to a whitening, moisturizing and anti-aging skin care product. The compound traditional Chinese medicine extract is prepared from the following raw material medicines in parts by mass: 10-50 parts of selaginella, 10-50 parts of peach blossom, 10-50 parts of bletilla and 10-50 parts of pseudo-ginseng. The compound traditional Chinese medicine extract which is prepared from the raw materials has an effect of suppressing tyrosinase and proliferation of melanocyte, has a superior whitening effect, has an effect of proliferating and promoting fibroblast and has an excellent effect of promoting generation of ceramide. The whitening, moisturizing and anti-aging skin care product which is obtained by applying the compound traditional Chinese medicine extract to preparing the whitening, moisturizing and anti-aging skin care product has a durable natural whitening effect and can be used for improvingmetabolism and moisturizing effects of skin.

The invention provides T cell receptors (TCRs) having antigenic specificity for a cancer antigen, e.g., tyrosinase. Also provided are related polypeptides, proteins, nucleic acids, recombinant expression vectors, isolated host cells, populations of cells, and pharmaceutical compositions. The invention further provides a method of detecting the presence of cancer in a host and a method of treating or preventing cancer in a host using the inventive TCRs or related materials.

The invention specifically discloses composite whitening and banishing essence and a preparation method thereof. The composite whitening and banishing essence comprises the following raw materials in percent by weight: 1 to 25 percent of Chinese herb active site extract, 0.1 to 10 percent of ethylhexyl ferulate, 0.1 to 5 percent of vitamin C ethyl ether, 0.05 to 3 percent of nicotinamide, 10 to 40 percent of grease, 1 to 6 percent of emulsifier, 1 to 15 percent of moisturizing agent, 0.1 to 1.5 percent of preservative, 0.1 to 0.5 percent of essence and the balance of deionized water. By virtue of the composite whitening and banishing essence, the activity of tyrosinase can be effectively inhibited, oxygen free radicals are efficiently eliminated, forming of melanin is prevented, and the formed melanin is decomposed and faded; meanwhile, an endocrine system of a human body can be regulated, and injuries to the skin caused by chemical components such as hormone are repaired, so that effects of treating both symptoms and root causes comprehensively and quickly are achieved.

The present invention describes compositions and methods for treating, preventing and improving hyperpigmentation, or other unwanted pigmentation of the skin, or other unwanted skin condition, such as age spots, aged skin, skin discoloration, etc., wherein the compositions include siRNA-gene silencing oligomers specific for tyrosinase. The compositions are used to treat a broad variety of pigmentation conditions, and are preferably applied to the skin, or are delivered by directed means to a site in need thereof.

The invention discloses a biological enzyme process antibacterial and anti-wrinkle finishing method of a silk fabric, belongs to the field of a textile biotechnology, and aims to overcome the defects of high baking temperature, high probability of damage of silk fibers and reduced whiteness in real silk antibacterial and anti-wrinkle treatment of the conventional chemical method and realize enzyme-promoted antibacterial and anti-wrinkle functional modification of a real silk fabric. According to the method, a chitosan natural finishing agent with a primary amine structure is grafted to the surface of the silk fiber by utilizing the catalytic oxidation effect of tyrosinase, so that the antibacterial and anti-wrinkle effects of the silk fabric are improved. The process flow comprises the following steps of: pre-treating the silk fabric; catalyzing grafting modification treatment of chitosan by the tyrosinase; washing; and drying. Through the silk fabric treated by the invention, the antibacterial performance is improved, the wrinkle recovery performance is improved, and the mechanical property and the dyeing performance of the fabric are also improved. Compared with the conventional chemical method, the method has the advantages of adoption of the tyrosinase process for catalysis of the antibacterial and anti-wrinkle treatment process of the real silk, low energy consumption, high efficiency, less pollution and contribution to environmental protection.

The invention relates to a reconstructed ovum of an albinism model pig and a construction method thereof, and a construction method of the model pig. According to the construction method of the reconstructed ovum, tyrosinase is knocked out to obtain the reconstructed ovum of the albinism model pig. The combination of a CRISPP / Cas9 gene knock-out technique and a somatic nucleus transplantation technique proves that the method for constructing the gene modified pig has high feasibility. The obtained TYR gene knock-out pig has the typical characteristics of albinism, can provide a reliable animal model for researching human albinism, can be applied to toxicity and anaphylaxis detection and many other aspects, and is hopeful to become a standardized experimental animal for albinism-like mice.

The invention discloses a tyrosinase electrochemical biosensor based on a metal organic skeleton material and chitosan and a preparation method and application thereof. The preparation method includes the steps that tyrosinase is first fixedly carried on the surface of the metal organic skeleton material (CuMOF) with copper ions being a metal center and then mixed with chitosan (Chi) to generate a CuMOF-Tyr-Chi composite material so that the composite material can be fixed to the surface of a glassy carbon electrode to prepare the tyrosinase electrochemical biosensor. The biosensor has the advantages that sensitivity for a bisphenol type compound is high; the detection limit is low; selectivity is good; the biosensor is easy to prepare without a complex sample pre-treatment process, response to a target compound is rapid, and miniaturization is easy.

The invention specifically discloses a composite whitening and spot correcting lotion, and a manufacturing method thereof. The spot correcting lotion is manufactured from raw materials of, by weight: 2.5 to 25 parts of traditional Chinese medicine active site extracts, 0.5 to 5 parts of arbutin, 0.05 to 0.2 parts of sodium hyaluronate, 5 to 10 parts of soybean fermentation extract, 1 to 10 parts of 1,3-butylene glycol, 1 to 10 parts of propylene glycol, 0.2 to 0.8 parts of tween-80, 0.1 to 0.4 parts of methyl-p-hydroxybenzoate, 0.1 to 0.4 parts of propylparaben, 0.5 to 5 parts pf ethanol, and 33.2 to 89.05 of deionized water. The traditional Chinese medicine active site extracts comprise raw materials of, by weight: 5 to 15 parts of ginseng, 5 to 20 parts of mongolian milkvetch root, 5 to 25 parts of white peony root, 5 to 30 parts of licorice, and 5 to 35 parts of cottonrose hibiscus flower. According to the composite whitening and spot correcting lotion provided by the invention, a plurality of traditional Chinese medicine active components are added to whitening and spot correcting active substances, such that the activity of tyrosinase can be effectively inhibited, oxygen-derived free radicals can be removed with high efficiency, and melanin can be prevented from appearing. With the composite whitening and spot correcting lotion, various freckles and chloasma can be comprehensively and rapidly corrected. Also, the lotion brings no toxin or harm.

The invention discloses biotechnology skin-whitening cosmetics. The biotechnology skin-whitening cosmetics comprise the following raw materials: ascorbic acid, arbutin, licorice extracts, punica granatum fruit fermentation product extracts produced by fermentation of lactobacillus, lemon extracts, rice chaff extracts, rose extracts, acerola cherry fermentation products produced by fermentation of the lactobacillus, gallnut extracts, oriental cherry extracts, inulin, mycose, ellagic acid and semen coicis extracts. The biotechnology skin-whitening cosmetics depend on pharmacology characteristics of bioactive substances produced by the modern biotechnology, the skin state of a user is conditioned and improved fundamentally, a skin-whitening effect is obvious, fading of foxiness and freckle can be facilitated, uneven skin tone is improved, skin brightness of the user is improved, and the skin-whitening effect is high. The biotechnology skin-whitening cosmetics can effectively restrain activity of monophenyl oxidase, act on various stages of a melanin metabolic path, restrain generation of melanin on the aspect of molecular level and whiten the skin of the user; and a skin-whitening effect is achieved.

The invention discloses a Chinese medicinal compound extract for whitening and removing freckles and a preparation method thereof, relates to a medicament for whitening and removing the freckles, and solves the problems of slow curative effect, low cure rate and inconvenient administration of Chinese medicaments for a long term in the treatment for chloasma at present. The Chinese medicinal compound extract for whitening and removing the freckles is prepared from bupleurum, Chinese angelica, white paeony root, dogwood, large-head atractylodes rhizome, Indian buead, szechuan lovage rhizome, peach seed, white muscardine silkworm, roasted ginger, mint and radix glycyrrhizae preparata; and the product is prepared by adopting carbon dioxide supercritical extraction technology. The method has the advantages of simpleness and convenient operation, and can highly enrich active ingredients. Therefore, the bioavailability is improved; the pharmacological activity is obviously enhanced; and the obtained product has small volume and does not contain solid impurities which cannot be emulsified. The Chinese medicinal compound extract effectively suppresses the activity of tyrosinase and melanogensis, removes or improves the chloasma, and responds within about one month, and the cure rate reaches 99.98 percent. The Chinese medicinal compound extract can be used as an active ingredient for directly preparing an external medicinal preparation and cosmetics for whitening and removing the freckles, is easy to carry, and is convenient to use.

The invention relates to a method for preparing alpha-arbutin which comprises steps of preparing slope seeds, selecting culture mediums, optimizing reaction conditions and extracting products and so on. The invention employs Xanthomonas campestris to ferment to produce the alpha-arbutin without use of organic solvents during the production and generation of harmful substances during the fermentation process. The invention has advantages of low production cost, simple process, and safe operation. And the alpha-arbutin yield produced by the method is high, the alpha-arbutin content in fermentation liquor is 8-11g / L, and the conversion rate of the hydroquinone can be 91%. The alpha-arbutin can obviously inhibit activities of tyrosinase, reduce tyrosinase deposition in skins, and bleach skins, remove freckles.

The invention relates to a composition with the efficacies of whitening, moisturizing, repairing and caring the skin, as well as a preparation method and applications of the composition. The skin care composition is prepared from the following raw materials: water, sodium hyaluronate, sodium dihydrogen phosphate dihydrate, potassium chloride, sodium hydroxide, calcium chloride, glutamine, benzalkonium chloride, magnesium sulfate, aminobutyric acid, sodium ascorbate, alanine, arginine, lysine hydrochloride, valine, histidine, leucine, taurine, coenzyme A, alcohol, polysorbate 80, thiamine, disodium diphosphate, recombinant human epidermal cell growth factors and the like. The skin care composition disclosed by the invention can permeate the muscle bottom for replenishing water for the skin from the deep layer, so that the skin is full of elasticity, the color of the skin is brightened, the fine wrinkles are reduced, the skin is clean and watery, the balance of water and oil is regulated, the pores are shrunk, meanwhile, the brightness of the face can be increased, the injured epidermal layer is effectively repaired, the activity of tyrosinase is inhibited, the generation of skin melanin is reduced, so that the skin is healthy, natural and white.

The invention relates to a plant extract and application of the plant extract in the field of a cosmetic with a whitening effect. The invention discloses a coreopsis tinctoria active part. A preparation method comprises the following steps of: taking coreopsis tinctoria dried powder, extracting with ethanol, combining filtrates, recovering a solvent in a decompressed manner, so as to obtain a coreopsis tinctoria crude extract; absorbing the coreopsis tinctoria crude extract through macroporous adsorption resin, eluting with the ethanol, collecting flow components, and evaporating to obtain the coreopsis tinctoria active part. With the adoption of the method, the coreopsis tinctoria active part is obtained and is the natural plant extract capable of restraining the tyrosinase. The coreopsis tinctoria active par has no side effect on a human body and can be used for preparing the cosmetic with the whitening effect.

The present invention discloses certain di- and polyvalent metal zeolite compounds (formula I) for topical delivery of biological and skin and hair care agents. The method of treating skin and hair condition via topical application of said zeolite compounds is also disclosed. The said method provides a treatment for topical condition, which includes alleviation of skin conditions such as skin rash including diaper rash, dry skin, scalp dandruff, broken or chafed skin, sunburn, skin damage from UV, skin irritation, acne including excess facial oil and facial pore size; darkened skin including age spots, dark circles around the eyes, and discoloration of skin from stretch marks; skin aging including wrinkles and fine lines; loss of collagen including thinning skin and loss of skin pliability; body odor, including oral cavity odor, arm-pit odor, and incontinence odor; cellular inflammation including intracellular and extra cellular inflammation; premature hair aging including premature hair loss hair graying; malfunction of tyrosinase group of enzymes, malfunction of matrix metalloprotease group of enzymes; and combinations thereof. The said method also provides topical delivery of certain metals, including trace metals, and certain zirconium aluminum amino acids that provide antiperspirant benefits;

The invention discloses an external preparation for treating vitligo and a preparation and using method thereof. The external preparation comprises the following components in portion by weight: 10 to 30 portions of radix salviae miltiorrhizae, 10 to 30 portions of rhizoma drynariae, 10 to 30 portions of herba ecliptae, 10 to 30 portions of angelica, 10 to 30 portions of dodder, 10 to 30 portions of white fructus tribuli, 10 to 30 portions of purslane, 10 to 30 portions of dark plum, 10 to 30 portions of cnidium fruit, 10 to 30 portions of prepared fleece-flower root, 10 to 30 portions of safflower, 10 to 30 portions of black sesame, 10 to 30 portions of szechuan lovage rhizome, 10 to 30 portions of gromwell root, 10 to 30 portions of honeysuckle, 10 to 30 portions of malaytea scurfpea fruit, 2 to 8 portions of radix euphorbiae ebractealatae, 10 to 15 portions of toad shell and 2 to 8 portions of chilopod. The external preparation can invigorate blood circulation and eliminate freckles, inhibit epidermis sulphydryl and melatonin, and activate the activity of tyrosinase so as to recover or accelerate the generation and the transfer of melanin and recover the color element of pathological skin; and the preparation method is simple, and can obtain good economic benefit.

The invention provides a traditional Chinese medicine facial mask with a whitening effect and a preparation method thereof. On the basis of the traditional Chinese medical science theory and with the purpose of skin health, the facial mask is prepared by using components, namely traditional Chinese medicine extracts as main functional additives and usually used matrix components in cosmetics. Through the traditional Chinese medicine facial mask with the whitening effect, the activity of tyrosinase is effectively inhibited. The traditional Chinese medicine facial mask has the effects of activating blood circulation and removing bloodstasis, nourishing blood and moisturizing skin, whitening and removing freckles, maintaining beauty, moistening skin and the like. The components, namely the traditional Chinese medicine extracts, of the traditional Chinese medicine facial mask are natural, green, healthy and safe and can be widely applied to whitening cosmetic products.