145 results about "Tyrosinase" patented technology

The invention belongs to the technical field of cosmetics, and particularly relates to a skin-color-uniformizing whitening composition derived from plant extracts and application of the skin-color-uniformizing whitening composition. The whitening composition provided by the invention is prepared from the following components in parts by weight: 10 to 15 parts of saussurea involucrata extract, 1 to 8 parts of dendrobium officinale extract, 1 to 5 parts of wood apple extract and 0.5 to 5 parts of banana fruit extract. The four plant extracts are synergistically compounded, transdermal absorption of active ingredients can be promoted, the tyrosinase inhibition rate is effectively increased, and the skin glossiness and whiteness are improved, so that the effects of whitening, brightening and uniformizing the skin color are achieved, the whitening composition is mild and non-irritant, the preparation method is simple and convenient to operate, and the whitening composition has large-scale production capacity and industrial feasibility. When the whitening composition is applied to skin care products such as face cream, the whitening and brightening effects of the face cream can be effectively improved, and the whitening composition has wide market application value.

The invention discloses an oligopeptide from undaria pinnatifida and application thereof. The amino acid sequence of the oligopeptide is YRKD, and the molecular weight is 580.63 g / mol. The polypeptide is identified from undaria pinnatifida through a high performance liquid chromatography technology for the first time, and an unreported oligopeptide capable of inhibiting tyrosinase and cooperating with vitamin C to synergistically inhibit tyrosinase is screened out. The oligopeptide derived from undaria pinnatifida is synthesized by a solid phase method, has the characteristics of safety, no toxicity, good water solubility and good stability, and is combined with vitamin C to generate a synergistic effect and synergistically increase the inhibition effect on tyrosinase, so that the oligopeptide can achieve a better tyrosinase inhibition effect at a lower and more stable concentration; the use concentration of the oligopeptide can be greatly reduced, the stability is enhanced, and the oligopeptide can be widely applied to preparation of cosmetics, medicines, health-care products or food additives, and has important economic value and social significance.

The invention discloses a strain of Blastococcus atamensis YC-1 and application of the strain of Blastococcus atamensis YC-1, and belongs to the field of microbial strains. The streptococcus sp. YC-1 provided by the invention has high antioxidant activity and also has the capability of producing DHHB (docosahexaenoic acid); meanwhile, the YC-1FLE is prepared by adopting absolute ethyl alcohol as a solvent, has no toxicity to HaCaT cells, is relatively high in reducing capacity, has a scavenging effect on DPPH free radicals, ABTS cation free radicals and hydroxyl free radicals, has a tyrosinase inhibition effect and shows good antioxidant and whitening effects; when the YC-1FLE is applied to the sunscreen lotion, the sunscreen coefficient of the sunscreen lotion containing the YC-1FLE is obviously higher than that of a blank control group, which shows that the YC-1FLE has better ultraviolet resistance. Therefore, the strain and the lysate extract of the fermentation product of the strain have huge application potential in the field of cosmetics.

The invention discloses schizophyllum commune as well as a product and application thereof, the schizophyllum commune is schizophyllum commune SY-2022-F11, and the preservation number of the schizophyllum commune SY-2022-F11 in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No. 42266. The schizophyllum commune is a schizophyllum commune strain. The schizophyllum commune is added into cosmetics in the form of schizophyllum commune fermentation liquor in the skin whitening process. The fermentation liquor of the schizophyllum commune SY-2022-F11 disclosed by the invention has relatively high tyrosinase inhibition rate and antioxidant activity.

The invention relates to a preparation method of citrus essential oil and a product and application thereof, the preparation method comprises the following steps: (1) mixing a citrus peel raw material with enzyme and water, and performing enzymolysis treatment to obtain an enzymolysis treatment solution; (2) performing high-voltage pulse electric field treatment on the enzymolysis treatment liquid to obtain solid-liquid mixed liquid; and (3) carrying out steam distillation on the solid-liquid mixed solution, collecting distillate, and separating an oil phase to obtain the citrus essential oil. The citrus essential oil is prepared by utilizing an enzymolysis-high-pressure pulse-steam distillation combined extraction method, the extraction process is low in condition, no organic reagent is introduced, the extraction process is green and environment-friendly, the loss of volatile active ingredients of the essential oil is less, and two treatments of enzymolysis and high-pressure pulse are added before steam distillation treatment, so that the yield of the essential oil can be increased; moreover, the tyrosinase inhibitory activity and the anti-free radical activity of the essential oil are also obviously improved, and the two pre-treatments promote each other and have a synergistic effect in the aspect of the effects.

The invention discloses spirulina protein-zein composite nanoparticles and a preparation method and application thereof.The preparation method comprises the steps that a zein solution is added into a spirulina protein solution, ethanol in the solution is removed through evaporation, insoluble substances are removed through centrifugation, and spirulina protein-zein composite nanoparticle dispersion liquid is obtained; and freeze-drying to obtain spirulina protein-zein composite nanoparticle powder. The preparation method of the spirulina protein-zein composite nano-particles is simple, low in cost, green and safe, and the obtained nano-particles can be kept stable in a neutral pH environment, have good biocompatibility, can be applied to efficient embedding of bioactive components, and have good application prospects. The light, heat and storage stability of the bioactive components are remarkably improved; and the oxidation resistance, tyrosinase inhibition and in-vitro release performance of the bioactive components are improved.

The invention discloses a design method and application of a tyrosinase (TYR) responsive drug for targeted therapy of melanoma, and belongs to the technical field of biological medicines. Based on the biological mechanism that TYR catalyzes tyrosine oxidation, a self-degradation connecting arm drug release strategy is combined, a similar 3-hydroxybenzyl alcohol structure is introduced to simulate a tyrosine substrate, specific recognition and activation of TYR on the drugs are achieved, and the problems that traditional chemotherapeutic drugs are poor in targeting property and serious in adverse reaction are hopefully solved.

The application belongs to the technical field of traditional Chinese medicines, and relates to an anti-inflammatory and whitening composition as well as a preparation method and application thereof. The composition comprises radix adiantidis, herba lepidagathis and rhizoma atractylodis. The radix adiantidis, herba lepidagathis and rhizoma atractylodis are selected as raw materials, and the three raw materials are scientifically compounded to achieve synergistic effect, so that the activity of tyrosinase can be efficiently inhibited, and the anti-inflammatory and whitening effects can be achieved. Through strict formula and process research, the raw materials in the composition are stable and compatible, the proportion is clear, and the safety is high, so that the problems of complex composition and large toxic and side effects of the composition are avoided.

The application provides a mussel mucin synthesis method and relates to the technical field of genetic engineering.The application improves the yield of mussel mucin by carrying out deletion mutation on the N-terminal sequence of the secondary structure of the mussel mucin amino acid sequence such as SEQ ID NO.1.The yield of mussel mucin in the prior art is about 2.57g / L, while the yield of mussel mucin in the application is about 5g / L, which is greatly improved compared with the yield of mussel mucin in the prior art.On this basis, the mussel mucin is modified by dopa through co-expression of tyrosinase, and the expression amount of the tyrosinase is controlled through different intensities of RBS, so as to regulate the modification degree of dopa, improve the yield of mussel mucin, and realize controllable modification of dopa in the mussel mucin, finally obtaining mussel mucin with high yield and different modification degrees of dopa.

The invention relates to a gene expression cassette and application thereof. The gene expression cassette comprises a tyrosinase gene, a transcriptional repressor protein gene, a melanin response promoter and a reporter gene, and the reporter gene is located at the downstream of the melanin response promoter and is regulated by the promoter. The gene expression cassette with a specific structure is designed, melanin in cells can be responded, a melanin biosensor can be further constructed by utilizing the gene expression cassette, the gene expression cassette has good specificity, and high-throughput screening of strains can be realized. Furthermore, ALE evolution is carried out on the strain for producing melanin, high-throughput screening is carried out based on a designed sensor, the strain for producing melanin with high yield is obtained, through shake-flask culture and 5L fermentation tank culture, the yield of melanin reaches up to 10.84 g / L and 29.04 g / L respectively, and an efficient and feasible solution is provided for large-scale production of melanin.

The invention discloses a compound with tyrosinase inhibitory activity and application of the compound in preparation of whitening products, and belongs to the technical field of medicinal chemistry. The compound is 5-(4-hydroxyphenyl)-3-O-glucosyl valeric acid, and is a novel compound. The compound can significantly inhibit the activity of tyrosinase, has the ability of blocking tyrosinase from catalyzing tyrosine to be converted into melanin precursor substances, and directly inhibits the generation of melanin, thereby showing better application prospects and values in skin whitening or skin diseases.

The invention provides a soft capsule helpful for improving the function of chloasma as well as a preparation method and application thereof, and relates to the technical field of health care products. The soft capsule comprises a mulberry leaf extract, a seville orange flower extract, a grape seed extract, an almond extract, donkey-hide gelatin powder, vitamin C and vitamin E. The soft capsule provided by the invention exerts the overall effect of improving chloasma through a multi-target synergistic mechanism from inside to outside: the mulberry leaf extract and the grape seed extract block melanogenesis from the source and resist ultraviolet damage by inhibiting tyrosinase activity and strongly resisting oxidation; the seville orange flower extract is used for soothing liver and regulating qi, and regulating internal pathogenesis; the almond extract and the donkey-hide gelatin powder have the effects of promoting blood circulation to remove blood stasis and nourishing yin and blood, improve microcirculation and nourish skin; vitamin C and vitamin E form an anti-oxidation cycle, so that melanin intermediates are directly reduced, and cell membranes are protected. All the raw materials supplement each other to jointly achieve the comprehensive effects of fading stains, brightening the skin color and improving the skin quality.

The invention belongs to the technical field of biological medicine manufacturing, and particularly relates to preparation and application of collagen peptide with whitening activity. The preparation method of the collagen peptide with the whitening activity comprises the following steps: step A, inoculating bacillus subtilis into a culture medium containing a moringa oleifera leaf extracting solution, fermenting for 6-12 hours, inoculating aspergillus niger, continuously fermenting for 36-48 hours, sterilizing, centrifuging, and taking supernate to obtain moringa oleifera leaf fermentation liquor; and step B, scaling the fish skin, cleaning, drying and crushing, adding the moringa oleifera leaf fermentation liquor, reacting for 6-10 hours at the pH value of 6.0-7.0 and the temperature of 35-45 DEG C, and performing ultrafiltration, filtrate concentration and freeze-drying to obtain the collagen peptide. The preparation process has the advantages of low cost, greenness and high efficiency, and the prepared collagen peptide shows the activity of inhibiting tyrosinase and can be applied to preparation of skin care products or medicines with a whitening function.

The application provides a method for preparing cross-linking enzyme based on a tyrosinase mutant, the cross-linking enzyme and application thereof. The tyrosinase mutant is mixed with an ultrasonic broken solution of S-adenosylmethionine synthetase containing a tyrosine tag to obtain water-insoluble cross-linking enzyme particles, the cross-linking enzyme is collected by centrifugation or filtration and the like, and enzymatic conversion is carried out. It is found that the cross-linking enzyme of S-adenosylmethionine synthetase can be continuously and stably used for 6 batches, and the activity retention is 85%. The cross-linking enzyme particles prepared by the method have the characteristics of easy recovery, reusability and low cost, and have good industrial application value.

This invention belongs to the technical field of daily-use shampoo and hair care raw materials, specifically relating to a composition with hair-darkening effects and its application. The composition with hair-darkening effects provided by this invention is formulated from a compound of Platycladus orientalis leaf extract, Polygonum multiflorum extract, Eclipta prostrata leaf extract, Gynostemma pentaphyllum extract, palmitoyl tetrapeptide-10, L-arginine, and decapeptide-4. By adjusting the types and amounts of each component, the interaction between the components is improved, thereby increasing the relative activity of tyrosinase and the relative content of melanin, indicating that the components have a synergistic effect and excellent hair-darkening efficacy. The hair-darkening composition obtained by this invention is applied to the preparation of shampoo, which exhibits excellent hair-darkening effects and high safety, and has broad market application value in the field of hair-darkening or hair-protecting products.

This invention provides a magnesium baicalein hydrogel with whitening and anti-skin aging effects and its preparation method. The formulation includes magnesium baicalein, sodium hyaluronate, carbomer, propylene glycol, niacinamide, a pH adjuster, and purified water. The hydrogel uses magnesium baicalein as the main active ingredient, overcoming the traditional deficiency of baicalein's poor water solubility and exhibiting good gel phase stability. The magnesium baicalein in the hydrogel can effectively scavenge free radicals and prevent collagen degradation to combat aging; simultaneously, it inhibits tyrosinase, synergistically achieving a dual whitening effect with niacinamide.

The disclosure provides methods for inhibiting: tyrosinase function and / or regulation or its activity, melanogenesis, melanin production, and cell proliferation of melanomas using compounds described here, or analog(s) thereof, or compositions comprising such compounds or analog(s) thereof in a therapeutically effective amount to bind or occupy any one or more of the tyrosinase binding sites in an amount sufficient to inhibit tyrosinase (i.e., function or regulation) or its activity, inhibit melanogenesis, inhibit melanin production, even out skin pigmentation or skin tone, brighten skin pigmentation or skin tone, inhibit cell proliferation of melanomas, and ameliorate or treat skin cancer (e.g., melanoma) in a subject in need thereof.

The present disclosure is directed recombinant T cell receptors capable of binding a tyrosinase epitope, a MAGA-A1 epitope, a MART1 epitope, a MAGE-A3 epitope, or an SSX2 epitope and nucleic acid molecules encoding the same. In some embodiments, the nucleic acid molecules further comprise a second nucleotide sequence, wherein the second nucleotide sequence or the polypeptide encoded by the second nucleotide sequence inhibits the expression of an endogenous TCR. Other aspects of the disclosure are directed to vectors comprising the nucleic acid molecule and cells comprising the recombinant TCR, the nucleic acid molecule, or the vector. Still other aspects of the disclosure are directed to methods of using the same. In some embodiments, the methods comprise treating a cancer in a subject in need thereof.

The present application relates to a kind of composition with whitening and anti-aging and its application, belong to the field of cosmetics.The composition with whitening and anti-aging of the present application includes the following mass parts of components: tetrahydro magnolia alcohol: 0.1-10 parts;Phytol: 1-5 parts;Resveratrol: 0.1-5 parts.The composition with whitening and anti-aging of the present application has significant inhibitory effect on tyrosinase and elastase, and has excellent whitening, anti-aging efficacy.

The invention relates to the technical field of daily chemical products, and provides an oxygen-enriched olive oil and DHA compounded whitening and repairing composition as well as a preparation method and application thereof, and the composition comprises DHA and oxygen-enriched olive oil in a mass ratio of 1: (1-100). By optimizing the ratio of the DHA to the oxygen-enriched olive oil, the synergistic combination of the excellent oxidation resistance of the DHA and the tissue repair and barrier conditioning capability of the oxygen-enriched olive oil is realized, the tyrosinase expression is down-regulated, the skin repair function is enhanced, the melanin degradation is promoted, and a good application prospect is achieved.

The invention discloses a multifunctional fermented soya bean polypeptide and application thereof, and relates to the field of food processing technologies and seasoning base materials. According to the invention, the multifunctional fermented soybean polypeptide (FGDEL) is obtained through screening. According to tests on oxidation resistance, Fe < 3 + > reduction capacity and tyrosinase inhibition capacity, the multifunctional fermented soya bean polypeptide has certain oxidation resistance, Fe < 3 + > reduction capacity and tyrosinase inhibition capacity, so that technical support is provided for developing oxidation-resistant products, products for improving iron-deficiency anemia (helping to improve iron element absorption) and whitening products.

The invention discloses a mask for extracting active substances of bombyx batryticatus under the assistance of enzymolysis and a preparation method of the mask, and belongs to the technical field of cosmetics. The preparation method comprises the following steps: crushing and degreasing stiff silkworms, and carrying out enzymolysis on the stiff silkworms by using a compound enzyme consisting of alkaline protease and flavourzyme under the conditions that the pH value is 7.5-9.5 and the temperature is 45-60 DEG C, so as to obtain an enzymolysis crude extract; carrying out fractionation through an ultrafiltration membrane, and collecting an active peptide enrichment liquid of a molecular weight segment of 3-10kDa; and finally, adding the active peptide enriched liquid into the mask matrix to prepare the smearing type gel mask. According to the method, composite enzymolysis and membrane separation purification are combined, the extraction rate of the bombyx batryticatus active peptide is remarkably increased, the obtained active peptide enrichment liquid has excellent antioxidant activity and tyrosinase inhibitory activity, sensitization protein and toxic small molecules are effectively removed, and safety is high. The mask disclosed by the invention has good multiple skin care effects of moisturizing, whitening and the like, and the process is simple and controllable.

The invention discloses a postoperative blackening inhibiting and brightening repairing body lotion and a preparation method thereof, the postoperative blackening inhibiting and brightening repairing body lotion comprises the following components by mass: 1-3% of tranexamic acid, 2-5% of nicotinamide, 3-6% of an aloe vera extract, 0.5-2% of menthol, 0.1-0.5% of an anti-allergy agent, 5-10% of a humectant, 2-4% of an emulsifier, 0.5-1% of a thickener, 0.05-0.2% of a pH regulator, 0.3-0.8% of a preservative, and the balance of deionized water. The invention has the following beneficial effects: by adding tranexamic acid into the formula, the activity of tyrosinase and melanocyte proliferation can be inhibited, melanin generation can be reduced from the source, nicotinamide can block melanin from being transferred to cuticle, and nicotinamide and tranexamic acid generate a synergistic effect, so that the blackness inhibition effect can be enhanced; the anti-inflammatory and moisturizing functions can be provided, then the postoperative skin inflammation reaction is reduced, menthol added in the formula can generate cooling feeling when activated, postoperative pain and scorching discomfort are effectively relieved, and the anti-allergy agent can reduce the skin allergy risk and improve the product safety.

The present invention provides tyrosinase-inhibiting molecules of general formula (I) and a dermopharmaceutical or cosmetic composition that includes at least one of said tyrosinase-inhibiting molecules.

This invention provides a composition that inhibits the tyrosinase enzyme involved in melanin production and also inhibits the action of collagen-degrading enzymes, thereby promoting collagen synthesis and achieving sufficient effects of skin whitening and elasticity improvement without inducing skin irritation due to skin allergies. [Solution] A composition having skin whitening and skin elasticity improving effects, according to one embodiment of the present invention, contains ginger extract as an active ingredient, and the ginger extract can be extracted using methylene chloride.

The invention discloses a short-chain melanin inhibitory peptide and application, the novel melanin inhibitory peptide has amino acid sequences of Cys-Tyr-Phe-Leu-Pro and Tyr-Gly-Phe-Leu-Pro-Cys, and the melanin inhibitory peptide has the characteristics of short sequence chain, high oxidation resistance, high melanin inhibitory activity, low toxicity and no hemolysis toxicity. The melanin inhibiting peptide can be used for preparing a melanin inhibiting preparation and has good inhibition on tyrosinase. The amino acid sequence of the melanin inhibiting peptide is greatly shortened, the production cost is greatly reduced, and the melanin inhibiting peptide has good biological safety, is not easy to cause drug resistance, is expected to become a novel melanin inhibitor, and has good application prospects in melanin inhibitors.

The application provides a 4-(2,4-dihydroxyphenyl)thiazole-2-carboxylic amide derivative and a preparation method and application thereof, and relates to the technical field of organic chemistry.The application provides a 4-(2,4-dihydroxyphenyl)thiazole-2-carboxylic amide derivative compound.The 4-(2,4-dihydroxyphenyl)thiazole-2-carboxylic amide derivative has the functions of inhibiting tyrosinase and melanin production, and has the characteristics of low toxicity and high safety.The preparation method can obtain the 4-(2,4-dihydroxyphenyl)thiazole-2-carboxylic amide derivative at a high yield.

This invention discloses a method for hydroxylation modification of recombinant mussel foot protein and its application. The invention utilizes a whole-cell catalyst with tyrosinase catalytic activity to hydroxylate tyrosine residues in recombinant mussel foot protein. This method eliminates the need for cell disruption and protein purification steps. By immobilizing tyrosinase on the host cell surface through cell surface display, the recombinant mussel foot protein can directly contact the tyrosinase in the extracellular environment and undergo hydroxylation, thereby introducing dopamine groups into the protein to improve its underwater adhesion properties. This method is applicable to the hydroxylation modification of various types of recombinant mussel foot protein and has advantages such as high catalytic efficiency, good stability, simple operation, and low cost, making it valuable for industrial applications.

The invention provides a synthetic method and application of silk fibroin-based melanin-like nanoparticles, and belongs to the technical field of nanoparticle preparations for biomedicine. After the silk fibroin nanoparticles, tyrosine and a tyrosinase solution are mixed, the silk fibroin-based melanin-like nanoparticles are obtained through a one-pot copolymerization reaction, and the enzyme concentration in the tyrosinase solution is 200-250 U / mL. The synthesis method has the advantages of being simple and convenient to operate, green, environmentally friendly and the like, and the synthesized silk fibroin-based melanin-like nanoparticles have excellent antioxidant activity and ultraviolet protection performance and remarkable skin cell photoaging resistance.

The application discloses a kind of alpha-lactalbumin multifunctional peptides with antioxidant, anticoagulant and tyrosinase inhibitory activity and application thereof, and belongs to the field of food, biopharmaceutical and cosmetic technology.The multifunctional peptide SEKLDQWLCEKL is screened, and the DPPH clearance rate and ABTS clearance rate of the multifunctional peptide SEKLDQWLCEKL are highest, which can reach 62.32% and 88.32% respectively, the clearance rate of hydroxyl radical is highest, which can reach 78.89%, PT and TT are respectively extended by 3.4 s and 4.7 s, and the inhibition rate of tyrosinase is highest, which can reach 89.07%, which overcomes the limitation of existing single functional peptide in application.