297 results about "Mucin" patented technology

The invention discloses a tissue adhesive based on mussel mucin as well as a preparation method and application thereof. The tissue adhesive is prepared from mussel mucin and an anionic compound containing a hydrophobic group, the mussel worm electrostatic self-assembly bionic composite material is developed on the basis of triple bionic principles of dopa adhesion of mussel byssus, electrostatic self-assembly polymerization of sandburg worms and hydrophobic acting force of barnacles. The preparation method of the tissue adhesive is simple, the tissue adhesive can be obtained only by mixing and oscillating the two stock solutions, the gel forming condition is simple and easy to implement, the pH range and the stock solution concentration range required by gel forming are large, the gel forming range is wide, the raw materials required in the preparation process are few, and the cost is low. Only two key raw materials including the mussel mucin and the anionic compound containing the hydrophobic group are needed, and the prepared tissue adhesive is high in adhesion speed and high in adhesion strength, has good cell compatibility and biological safety and can promote wound healing.

The invention relates to the technical field of grinding and crushing, and discloses hippocampus active mucoprotein grinding equipment and an extraction technology.The hippocampus active mucoprotein grinding equipment comprises a shell, a motor is fixedly installed at the bottom of the shell, a driving shaft is fixedly installed at the output end of the top of the motor, and a primary grinding assembly is detachably installed at the top end of the driving shaft; a secondary grinding assembly is fixedly installed on the circumferential face of the upper half portion of the driving shaft, an annular sieve tray is fixedly installed on the inner wall of the middle of the shell, and a vibration assembly is arranged on the surface of the driving shaft. The shoveling plate is driven by the rotating cover to move at the top of the annular screening disc, hippocampus on the bottom layer of the annular grinding groove is thrown upwards, the hippocampus enters the space between the hollow rotating roller and the outer convex ring and the space between the hollow rotating roller and the inner convex ring, and the hollow rotating roller rotates while moving along the annular grinding groove. The outer wall of the hollow rotating roller is matched with the outer convex ring and the inner convex ring to extrude and crush the hippocampus, so that secondary grinding of the hippocampus is realized; therefore, the purposes of grinding the hippocampal tissue twice and improving the grinding efficiency are achieved.

The invention relates to mussel mucin-hyaluronic acid nanogel and a preparation method and application thereof and a pharmaceutical composition, and belongs to the field of biomedical materials, the structural formula of the mussel mucin-hyaluronic acid nanogel is # imgabs0 #, Mussel mucin is a mussel mucin molecule, and n is the number of repetitive units. The mussel mucin-hyaluronic acid nanogel can be swelled in a water-based medium to form a three-dimensional space net-shaped structure, pharmaceutical active molecules are better entrapped, lysine and dopa groups in the structure can be tightly combined with the surface or mucous membrane of an organism through electrostatic interaction, hydrogen-bond interaction and oxidative crosslinking, so that the bioavailability of the mussel mucin-hyaluronic acid nanogel is improved, and the bioavailability of the mussel mucin-hyaluronic acid nanogel is improved. The carrier has good biological adhesion, can prolong the residence time of the carrier on an organism or mucous membrane surface, and promotes drug absorption. The mussel mucin-hyaluronic acid nanogel can also be used as a drug storage cavern, so that the slow release effect of drugs is improved, the action time of the drugs is prolonged, and the administration frequency is reduced.

The invention discloses a preparation method of high-purity recombinant mussel mucoprotein, and belongs to the technical field of recombinant protein preparation, the idea of improving target protein expression by adopting fusion protein is adopted, and fusion expression is performed on partial peptide fragments of an amino acid sequence of mytilus coruscus Mfp-3 and an amino acid sequence of common mussel preCol-D to obtain the high-purity recombinant mussel mucoprotein. The recombinant mussel mucin which is high in purity, low in endotoxin content and good in adhesion performance is obtained by combining improvement of induction, tyrosinase modification and separation and purification processes, a new thought is provided for industrial production of the recombinant mussel mucin through successful preparation of the recombinant mussel mucin, raw material support is provided for multi-field application of the recombinant mussel mucin, and the method is suitable for industrial production of the recombinant mussel mucin. The customized sequence recombinant mussel mucoprotein is prepared according to application requirements, and the application of the mussel mucoprotein in different fields is promoted.

The invention relates to the technical field of hydrogel, in particular to mussel mucoprotein loaded extracellular vesicle hydrogel as well as a preparation method and application thereof. The hydrogel is prepared by adopting a mode of combining human umbilical cord mesenchymal stem cell exosome Exo and mussel mucoprotein MAP; the method has the following advantages: 1, the wound repair effect is improved: the biological activity of the exosome Exo and the adhesion performance of the mussel mucin MAP are utilized to jointly promote the wound repair process, so that the repair effect is improved; 2, the adhesion performance is enhanced: the strong adhesion of the mussel adhesive protein MAP is utilized to ensure that the hydrogel can be tightly attached to the wound surface in a wet environment, and the dressing is prevented from falling off and infection risk; and 3, multifunctionality is realized: the composite hydrogel not only has the basic functions of promoting healing, resisting bacteria and the like, but also can transmit genetic information through exosome Exo, so that tissue regeneration and skin barrier repair are further promoted.

The invention belongs to the technical field of gel dressings, and particularly relates to a bio-based gel dressing for wound treatment and a preparation method thereof.The bio-based gel dressing is prepared from, by weight, 10-17 parts of mussel mucin, 13-20 parts of silk fibroin, 1-5 parts of plant vesicles, 2-4 parts of traditional Chinese medicine polysaccharide, 0.05 part of antibacterial peptide, 1.5 parts of glycerinum and 0.3 part of cross-linking agent; nano plant vesicles which are extracted from a traditional Chinese medicine prescription and have the effect of promoting wound healing are used as a carrier for encapsulating antibacterial peptide, and then the antibacterial peptide is encapsulated in a three-dimensional gel network formed by natural protein, so that controlled release and slow release of antibacterial and anti-inflammatory components are realized, and efficient wound healing is promoted.

The present disclosure relates to 3-substituted 1,2,4-oxadiazole compounds and their derivatives, which are useful as T-cell immunoglobulin and mucin-domain containing-3 (TIM-3) inhibitors or as dual inhibitors of TIM-3 and the programmed cell death 1 (PD-1) signaling pathway. The disclosure also relates to treatment of disorders by inhibiting an immunosuppressive signal induced by TIM-3, PD-1, PD-L1, and / or PD-L2.

The invention is suitable for the technical field of gynecological gels, and provides a gynecological gel with a repairing effect. Comprising the following components in percentage by weight: 0.1%-0.5% of recombinant III type human collagen, 0.05%-0.2% of mussel mucin, 1%-3% of chitosan, 0.2%-1% of sodium hyaluronate, 2%-5% of lactobacillus fermentation product filtrate, 0.05%-0.2% of tea tree essential oil, 0.2%-0.5% of carbomer, 0.1%-0.4% of triethanolamine, 0.1%-0.2% of sodium methylparaben and the balance of purified water. The gynecological gel with the repairing effect has the excellent repairing effect, integrates multiple effects of effectively resisting bacteria and diminishing inflammation, promoting repairing of damaged vaginal mucosa, adjusting vaginal micro-ecological balance and the like, not only can efficiently inhibit growth of various pathogens, but also can remarkably promote proliferation and repairing of vaginal mucosa cells, and has the advantages that the repairing effect is good, and the repairing effect is good. The vaginal micro-ecological balance can be accurately adjusted, no stimulation is caused to vaginal mucosa, safety and reliability are achieved, and long-term use can be achieved.

The present invention provides a stable composition containing mussel mucin, which has the characteristics of good stability, high DOPA content, and not easy to change color. The present invention also provides a preparation method of the preparation, which improves the DOPA stability of mussel mucin by adding specific components and improves the yellowing of color.

The invention provides a nano transdermal sustained-release biological scaffold as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The nano transdermal sustained-release biological scaffold provided by the invention comprises the following components: a load and a nano carrier, the load comprises mussel mucin, trehalose and Ectoine; the mass ratio of the mussel mucin to the trehalose to the Ectoine is (4 to 6) to (0.5 to 1.5) to (0.5 to 2.0). Through the innovative design of a quaternary composite system of mussel mucin, trehalose, Ectoine and a nano-carrier, the three technical problems of short slow release period, insufficient targeting property and difficulty in large-scale production in a wound repair material in the prior art are successfully solved.

The invention relates to the technical field of biomedical materials, in particular to an oral repair film and a preparation method and application thereof. The oral repair membrane comprises an electrostatic spinning membrane with the porosity of 30%-40%, and polymer microspheres, recombinant humanized collagen and mussel mucoprotein which are attached to the electrostatic spinning membrane, wherein the particle size of the polymer microspheres is 25 [mu] m-50 [mu] m. According to the oral cavity repairing film provided by the invention, by combining a polymer electrostatic spinning technology, a shearing and emulsifying microsphere preparation technology, a microsphere slow release technology and a three-dimensional porous bracket freeze-drying technology, the problems that a repairing film in the marketed or published technology is small in contact area and needs to be sewn are solved; the tissue regeneration can be efficiently stimulated, the treatment time is shortened, the treatment effect is improved, suturing is not needed, and clinical operation is facilitated.

The invention discloses a medical wound surface composite freeze-dried dressing and a preparation method thereof, and relates to the field of medicines. The medical wound surface composite freeze-drying dressing comprises a freeze-drying product and a solvent which are independently stored, the freeze-drying product comprises recombinant mussel mucin, recombinant collagen and a freeze-drying protective agent, and the solvent comprises an osmotic pressure regulator and water. By utilizing the synergistic effect of the recombinant collagen and the recombinant mussel mucin, the wound repair effect of the medical wound composite freeze-dried dressing is improved.

Provided herein are compositions, methods, and uses involving anti-Mucin-16 (MUC16) agents that immunospecifically bind an epitope of Mucin-16 (MUC16). Also provided herein are uses and methods for managing, treating, or preventing disorders, such as cancer and diseases associated with positive MUC16 expression.

The invention provides hydrogel with a wound healing function and a preparation method thereof.The hydrogel is prepared from, by mass, 50-70 parts of basic gel matrix, 20-30 parts of natural medicine extract and 8-10 parts of bioactive ingredients; the natural medicine extract comprises superfine pearl powder, a semen cassiae extract, an aloe extract, a cortex phellodendri extract, a honeysuckle flower extract, a dandelion extract, a centipede extract, a fructus forsythiae extract and a green tea extract; the bioactive components comprise epidermal growth factors, heparin sodium, mussel mucin and nano-silver ions; on the basis of a commercially available compound cortex phellodendri liniment, further research and development are carried out, the superfine pearl powder, the semen cassiae extract, the aloe extract, the green tea extract and other natural medicine components are added, the optimal proportion is selected, and the basic gel matrix, the bioactive components and the like are combined, so that the wound healing rate, the antibacterial rate and the water holding rate of the hydrogel are remarkably improved finally.

The invention discloses a rapidly cross-linked recombinant mussel adhesive protein adhesive as well as preparation and application thereof, and relates to the technical field of biological adhesives. According to the preparation method of the rapidly cross-linked recombinant mussel mucin adhesive, by changing the cross-linking mode of the recombinant mussel mucin, the dosage of the catalyst can be reduced, rapid cross-linking can be realized, and the mechanical property and viscoelasticity of the adhesive can be regulated and controlled according to requirements in the preparation process.

The invention discloses a preparation method of recombinant mussel mucin rich in dopa, and belongs to the technical field of genetic engineering. When the recombinant mussel mucoprotein is co-expressed and modified, a certain amount of modifier is fed in the induction process according to the growth of thalli and the expression condition of protein, so that on one hand, the component concentration of a co-expression modification system is controlled, and a favorable environment is provided for the recombinant tyrosinase to oxidize tyrosine into DOPA; on the other hand, an environment is created for the synthesized recombinant mussel mucin containing dopa, the situation that dopa residues are oxidized into unnecessary substances such as dopaquinone and melanin due to long co-expression modification time is avoided, glutamic acid with a certain concentration in a modification auxiliary system serves as energy supply to promote modification, and the dopa oxidation rate and modification efficiency are improved.

Provided herein are antibodies, or antigen-binding portions thereof, that bind to T-cell immunoglobulin and mucin-domain containing-3 (TIM3) protein. Also provided are uses of these antibodies, or antigen-binding portions thereof, in therapeutic applications, such as treatment of cancer. Further provided are cells that produce the antibodies, or antigen-binding portions thereof, polynucleotides encoding the heavy and / or light chain regions of the antibodies, or antigen-binding portions thereof, and vectors comprising the polynucleotides encoding the heavy and / or light chain regions of the antibodies, or antigen-binding portions thereof.

The present disclosure relates to 3-substituted 1,2,4-oxadiazole compounds and their derivatives, which are useful as T-cell immunoglobulin and mucin-domain containing-3 (TIM-3) inhibitors or as dual inhibitors of TIM-3 and the programmed cell death 1 (PD-1) signaling pathway. The disclosure also relates to treatment of disorders by inhibiting an immunosuppressive signal induced by TIM-3, PD-1, PD-L1, and / or PD-L2.

The application discloses a preparation method of high-purity recombinant mussel adhesive protein and belongs to the technical field of recombinant protein preparation. The amino acid sequence of Mfp-3 of Mytilus coruscus and the partial peptide segment of the amino acid sequence of preCol-D of Mytilus edulis are fused and expressed according to the idea of improving target protein expression by using fusion protein, and the improvement of the induction, tyrosinase modification and separation and purification process is combined to obtain the recombinant mussel adhesive protein with high purity, low endotoxin content and good adhesion performance. The successful preparation of the recombinant mussel adhesive protein provides a new idea for the industrial production of the recombinant mussel adhesive protein, provides raw material support for the multi-field application of the recombinant mussel adhesive protein, realizes the preparation of the customized sequence recombinant mussel adhesive protein according to application requirements, and promotes the application of the mussel adhesive protein in different fields.

The invention relates to the field of molecular biology, and discloses a nucleic acid releasing agent for isothermal amplification and a use method of the nucleic acid releasing agent. Comprising the following steps: step 1, preparing a polyoxypropylene polyoxyethylene copolymer diluent, a defoaming agent SE-15 diluent, an ethyl phenyl polyethylene glycol NP-40 diluent, a sodium polyethylene sulfonate diluent and a Proclin300 diluent in sequence through RNA enzyme-free water; and 2, sequentially preparing an ethylenediamine tetraacetic acid disodium salt mother solution, a sodium dodecyl sulfate mother solution and a glycine mother solution through RNA enzyme-free water. Sodium polyethylene sulfonate can be specifically combined with protein amplification inhibitors in a sample to remove mucoprotein, hemoglobin, polysaccharide and other inhibitors in the swab sample, and disodium ethylene diamine tetraacetate can chelate metal ions to reduce the influence of nuclease on target nucleic acid.

The invention relates to a recombinant mussel-like mucoprotein as well as a coding gene and application thereof, and belongs to the technical field of gene engineering and protein engineering. According to the invention, partial functional sequences of different types of mussel mucin are screened, assembled and spliced to obtain a section of brand new mussel mucin-like sequence, and the sequence is optimized by escherichia coli codon preference to construct a high-expression recombinant strain. According to the recombinant strain, the soluble expression quantity of the target protein is remarkably improved by optimizing culture conditions. The recombinant protein does not carry any exogenous tag, and the provided purification method is low in cost, high in yield and suitable for large-scale production. The prepared recombinant mussel-like protein has good adhesiveness and oxidation resistance, and the modified recombinant mussel-like protein is further provided, and the biological performance of the modified recombinant mussel-like protein is better than that of unmodified recombinant mussel-like protein.

The invention relates to the technical field of gynecological gel, and discloses gynecological gel containing recombinant mussel mucin as well as a preparation method and application of the gynecological gel. The gynecological gel containing the recombinant mussel mucin comprises the following raw material components in percentage by mass: 0.01%-50% of the recombinant mussel mucin, 0.1%-20% of a gel matrix, 0.01%-1.0% of a pH regulator, 0.05%-1% of a preservative, 0.5%-20% of a humectant, 10%-50% of a protein protective agent and the balance of water, the gel matrix comprises a thickening agent and a suspending aid, and the protein protective agent is obtained by dissolving a dispersing agent, a chelating agent and a protein stabilizer in a buffer solution. The gynecological gel provided by the invention has good film-forming property, forms a protective gel film on a vaginal wall, physically isolates the vaginal wall from external bacteria, is used for protecting gynecological mucosa and preventing colonization of pathogenic microorganisms, has good anti-inflammatory and anti-oxidation effects, and can be used for preventing or treating gynecological diseases.

The invention relates to the technical field of protein design, and particularly discloses an Mfp-SOD recombinant protein as well as a preparation method and application thereof. According to the recombinant protein, mussel mucin Mfp-3 is used as a skeleton to assist anchoring of human-derived protein SOD, and a novel recombinant protein is obtained. According to the recombinant protein, two proteins are fused, so that the recombinant protein has excellent skin surface affinity and the functions of oxidation resistance, aging resistance, whitening, spot fading, inflammation resistance, sunscreen and the like, and the effects of oxidation resistance and aging resistance can be exerted for a long time.

The invention relates to Ackermania muciniphila and application thereof, and belongs to the technical field of microorganisms. According to the present invention, the ackermania muciniphilaSYSU-85 is adopted as the ackermania muciniphilaSYSU-85, and has been preserved in the China General Microbiological Culture Collection Center (CGMCC) on August 8, 2024, and the preservation number of the ackermania muciniphilaSYSU-85 is CGMCC NO.46070; the Ackermania muciniphila SYSU-85 disclosed by the invention is weak in dependence on mucin, grows well in a BHI culture medium added with cysteine, and is resistant to oxaliplatin. The Ackermansiella muciniphila SYSU-85 provided by the invention alleviates adverse reactions caused by oxaliplatin, and is combined with oxaliplatin or a PD-1 inhibitor to significantly slow down a tumor growth curve and significantly reduce the tumor volume, thereby improving the tumor treatment effect.

The invention relates to the technical field of medical dressings, and discloses a scar repair gel containing recombinant mussel mucin and a preparation method thereof. The scar repair gel is prepared from the following components in percentage by mass: 0.01 to 0.2 percent of recombinant mussel mucin, 0.4 to 0.8 percent of a thickening agent, 6 to 15 percent of a compound emulsifier, 1 to 2 percent of a pH regulator, 20 to 40 percent of silicone, 0.1 to 0.3 percent of a preservative and the balance of water, wherein the silicone comprises polydimethylsiloxane, a polydimethylsiloxane cross-linking agent and decamethylcyclopentasiloxane. The scar repair gel containing the recombinant mussel mucin has good film-forming property, forms a moisturizing barrier on the surface of a wound, can inhibit formation of hyperplasia scars, reduces pruritus in the wound healing process, reduces generation and deposition of melanin, and has the effects of repairing scars and repairing scars. The comfort of a patient in the use process and the compounding stability of the mussel mucin and silicone gel system are effectively improved.

The invention discloses a probiotic / mucoprotein loaded oral microsphere system as well as a preparation method and application thereof, and belongs to the field of compositions. The preparation method of the system comprises the following steps: dissolving probiotics, Mucin and HAMA in double distilled water containing phenyl lithium phosphate LAP and calcium chloride to prepare a gel matrix solution; adding the dissolved gel matrix liquid into an oil phase solvent, and stirring at a high speed in a container; after uniformly stirring, carrying out an ultraviolet light crosslinking reaction, and after the reaction is finished, fully cleaning the obtained microspheres to remove the redundant oil phase solution, so as to obtain microspheres; and dispersing the obtained microspheres in a sodium alginate solution under an acidic condition, uniformly mixing, carrying out a cross-linking reaction, and filtering and cleaning after the reaction is finished, so as to obtain the probiotic / mucoprotein loaded oral microspheres. According to the invention, HAMA and SA are utilized to prepare an oral microsphere system resistant to gastric acid to co-deliver Mucin and EcN, so as to realize continuous and effective regulation and control of unbalanced intestinal flora by probiotics.

Disclosed herein are double stranded ribonucleic acids (dsRNAs) and synthetic antisense oligonucleotides (ASOs) for use in inhibiting the expression of MUC5B and in the treatment of a subject having a disease or disorder characterized by overexpression of MUC5B, including in subjects having lung disease or in a subject at risk of developing lung disease, for example, in a subject having or at risk of developing idiopathic pulmonary fibrosis.

The invention relates to the technical field of medical dressings, and discloses a compound hydrogel dressing based on recombinant mussel mucin and a preparation method of the compound hydrogel dressing. The compound hydrogel based on the recombinant mussel mucin is prepared from the following raw material components in parts by weight: 0.01 to 0.3 part of the recombinant mussel mucin, 0.1 to 2.0 parts of a thickening agent, 10 to 21 parts of a polysaccharide composition, 1 to 8 parts of a humectant, 0.01 to 0.5 part of a pH (Potential of Hydrogen) regulator, 0.05 to 0.2 part of a preservative, 0.05 to 0.2 part of a metal ion chelating agent and 68 to 88 parts of purified water, the polysaccharide composition is prepared from glucan, chitosan and hyaluronic acid. The polysaccharide is compounded into the hydrogel of the recombinant mussel mucoprotein to play a role synergistically, and the polysaccharide and the recombinant mussel mucoprotein are closely combined and cross-linked, so that the adhesion of the compound hydrogel dressing is effectively improved, wound healing is promoted, the anti-inflammatory effect is improved, and meanwhile, a good protective barrier environment is formed for wound repair and healing.

The application relates to the technical field of protein design, and particularly discloses a kind of Mfp-SOD recombinant proteins and a preparation method and application thereof.The recombinant protein takes the mussel mucin Mfp-3 as a skeleton, assists anchoring human-derived protein SOD, and obtains a new type of recombinant protein.The recombinant protein is fused by two kinds of proteins, has excellent skin surface affinity and functions of antioxidation, anti-aging, whitening, spot-fading, anti-inflammation and sun protection, and can achieve long-acting antioxidation and anti-aging effects.

The application provides a mussel mucin synthesis method and relates to the technical field of genetic engineering.The application improves the yield of mussel mucin by carrying out deletion mutation on the N-terminal sequence of the secondary structure of the mussel mucin amino acid sequence such as SEQ ID NO.1.The yield of mussel mucin in the prior art is about 2.57g / L, while the yield of mussel mucin in the application is about 5g / L, which is greatly improved compared with the yield of mussel mucin in the prior art.On this basis, the mussel mucin is modified by dopa through co-expression of tyrosinase, and the expression amount of the tyrosinase is controlled through different intensities of RBS, so as to regulate the modification degree of dopa, improve the yield of mussel mucin, and realize controllable modification of dopa in the mussel mucin, finally obtaining mussel mucin with high yield and different modification degrees of dopa.