The invention belongs to the technical field of a microbial medium, and specifically discloses a Bacillus thuringiensis israelensis medium. The weight percentage of each component is: 0.5-1% of sucrose, 0.6-1.2% of corn flour, 1-5% of bean cake powder, 0.1-0.5% of peptone, 0.4-1.2% of corn syrup, 0.1-0.5% of calcium carbonate, 0.05-0.1% of potassium dihydrogen phosphate, 0.05-0.1% of magnesium sulfate, 0.05-0.1% of zinc sulfate, 0.1-0.3% of sodium chloride, 0.01-0.05% of potassium nitrate, and 89.95 to 97.04% of deionized water. The invention also discloses an application of the above medium in the Bacillus thuringiensis israelensis. The medium can promote the rapid entry of Bacillus thuringiensis israelensis into a logarithmic growth phase, the proportion of viable spores of a fermentation broth is more than 90%, and the virulence to the Aedes aegypti and Culex pipiens pallens larvae is high, the medium greatly improves the product quality and reduces production costs, and is suitablefor large-scale cultivation and production.