The invention discloses a preparation method of liquid-state fermented
gastrodia elata, and relates to the technical field of microbial ferment.The preparation method of the liquid-state fermented
gastrodia elata comprises the following steps that S1,
raw material pretreatment is conducted, specifically,
mildew-free
gastrodia elata is selected, cleaned and then dried at the low temperature (50 DEG C), the
gastrodia elata is smashed to be 60 meshes, strain
liquid nitrogen freezing preservation is conducted, and before use, a PDA slant culture medium is used for activation; s2, a culture medium is prepared, wherein the
gastrodia elata powder (60 meshes) accounts for 30%, glucose (10%),
corn steep liquor (5%), KHPO (0.1%) and MgSO (0.05%) are supplemented, and the initial pH is adjusted to 6.0; the culture medium is subjected to high-temperature instantaneous sterilization (135 DEG C, 5 seconds),
nutrient loss is avoided, meanwhile, seed liquid is pumped into a
fermentation tank according to the
inoculation amount of 10%, and sterile operation is conducted in the whole process; according to the preparation method of the liquid-state fermented
gastrodia elata, the
fermentation efficiency is remarkably improved, the production period is shortened, the
gastrodin concentration is increased, meanwhile, residual components in thalli are released through a high-pressure homogenizing and crushing technology, and the
utilization rate of raw materials is increased.