The invention discloses a
protein molecular
weight distribution detection method based on a gel
filtration technology, and the method comprises the following steps: building a
protein molecular weight detection technology platform based on the gel
filtration technology, and detecting the characteristic peaks or characteristic sections and light absorption values of milk and dairy product proteins under a specific
wavelength in real time; and designing and proposing an evaluation measure sigma / sigma 0, a section ratio and applicable conditions and ranges for calculating the molecular
weight distribution of the
protein, and detecting and analyzing the molecular
weight distribution of the protein. The molecular weight distribution measure is designed to describe the concentration distribution and change of proteins with different molecular weights, so that the
protein coagulation / degradation degree of the milk and the dairy products is evaluated, the quality of the milk and the dairy products is identified, and the dairy products and non-dairy products are distinguished. Establishment of the method is helpful for flexibly and conveniently evaluating and monitoring the quality of milk and dairy products in a plurality of application scenes such as storage and
shelf life tracking.