This invention discloses a method for preparing
protein powder through low-temperature separation, comprising the following steps: S1:
raw material pretreatment; S2: low-temperature
defatting; S3: multi-stage low-temperature membrane separation; S4: low-temperature concentration; S5: freeze-
drying; S6: post-treatment. This invention uses animal-derived whey as
raw material, sequentially performing
raw material pretreatment, low-temperature
defatting, multi-stage low-temperature membrane separation, low-temperature concentration, freeze-
drying, and post-treatment. The temperature is controlled within the range of 0–10°C throughout the process. By optimizing the pretreatment process to remove impurities and heat-sensitive interfering substances, and employing a combination of gradient-pore-size
ceramic membranes and
ultrafiltration membranes, efficient separation and purification of proteins are achieved. Combined with vacuum low-temperature concentration and freeze-
drying technology, the
active components of
whey protein are preserved to the maximum extent. This invention solves the problems of large
protein activity loss, low purity,
high energy consumption, and easy introduction of
chemical contaminants in existing preparation methods.