156 results about "Galactooligosaccharide" patented technology

The invention discloses a composition for regulating sleep rhythm homeostasis as well as a preparation method and application thereof. The composition for regulating the sleep rhythm homeostasis is prepared from the following components in percentage by weight: 5 to 18 percent of polydextrose, 9 to 15 percent of galactooligosaccharide, 1 to 10 percent of inulin, 0.4 to 2 percent of gamma-aminobutyric acid, 0.8 to 2.2 percent of L-theanine, 0.1 to 3.5 percent of magnesium citrate, 2 to 21 percent of semen ziziphi spinosae extract, 1 to 10 percent of poria cocos extract, 0.5 to 2 percent of pericarpium citri reticulatae extract, 0.3 to 1 percent of liquorice root extract and 15 to 40 percent of maltodextrin. 5-18% of coconut oil powder MCTs, and 0.5-2% of a lily extract. According to the application, a magnesium MCTs shell-core structure is delivered by maltodextrin to form a mitochondrial piezoelectric engine so as to improve prebiotics, promote flora metabolism efficiency and energy supply feedback, activate intestinal pheochromophilic cells and improve SLC6A4 gene expression so as to improve synthesis of 5-hydroxytryptamine in the brain and activate GABA energy neurons; meanwhile, through the spina date seed and poria cocos quantum dots and a pericarpium citri reticulatae and liquorice pi-pi stacking network, the blood-brain barrier penetration rate is greatly increased, and hypothalamic CRH release is adjusted.

The invention relates to the technical field of functional food, in particular to immune regulation type prebiotic syrup with synergy of galactomannan. The active components of the composite solid beverage are prepared from the following raw materials in percentage by weight: 20%-35% of fructo-oligosaccharide, 10%-18% of galactooligosaccharide, 8%-15% of stachyose, 3%-7% of xylooligosaccharide, 5%-12% of lactitol, 5%-10% of galactomannan, 2%-5% of yeast beta-glucan and 1%-3% of N-acetylglucosamine. The composition further comprises 4-8% of inulin and 3-6% of resistant dextrin as a matrix filling and slow-release carrier, and the sum of the weight percentages of all the active ingredients and the carrier is 100%. By remodeling intestinal microecology and inhibiting inflammation pathways, the intestinal inflammation state can be remarkably improved, expression of intestinal mucosa tight junction protein is promoted, and an innovative solution is provided for people suffering from chronic bowel diseases and immune imbalance.

The invention relates to the technical field of prebiotics preparation, and discloses a preparation method of prebiotics applied to reproduced areca catechu, and the preparation method comprises the following steps: carrying out enzyme catalysis reaction on sucrose by adopting fructosyl transferase to prepare fructo-oligosaccharide; according to the method, beta-galactosidase is adopted to carry out enzyme catalysis reaction on lactose, so that galactooligosaccharide is prepared; respectively carrying out decoloration, ion exchange, nanofiltration concentration and spray drying on the candy oligosaccharide and the galactooligosaccharide to obtain powder; the method comprises the following steps: mixing fructo-oligosaccharide powder and galactooligosaccharide powder according to a ratio, and uniformly distributing the mixture in a reproduced areca-nut matrix by adopting a spray-drying blending or low-temperature vacuum mixing mode to form a functional reproduced areca-nut product; according to the invention, low-temperature drying is carried out on the product after blending or vacuum mixing, so that the prebiotic areca nuts are released along with saliva in the chewing process, and are selectively utilized by bifidobacteria and lactic acid bacteria after entering the stomach and intestine, thereby achieving the purposes of regulating intestinal flora and improving the health-care function.

The invention discloses a preparation method of soybean milk powder for promoting immune regulation, and belongs to the technical field of soybean product processing. Performing biological enzymolysis; modifying soybean protein; compounding functional components; and preparing the microcapsule. An ultrasonic-enzyme composite extraction technology is applied, and the enrichment rate of components with immunocompetence is increased. According to the method, the Maillard reaction is performed on the soybean protein and the polysaccharide by utilizing an ultrasonic-assisted heating technology, so that the emulsifying characteristic of the soybean protein isolate is improved, and the immunoregulation promoting performance of the soybean protein isolate is greatly improved. According to the invention, through a microcapsule embedding technology, glycosylated soybean protein is wrapped in the particles by a wall material formed by galactooligosaccharide and resistant dextrin, the stability and bioavailability are improved, the microencapsulation efficiency is greater than 95%, and the immunoregulation promotion performance of the microencapsulated soybean protein is effectively exerted. By adopting spray drying, inactivation of immune active components caused by high temperature is avoided.

The invention belongs to the technical field of biochemistry, and particularly relates to a probiotic composition for relieving depression as well as a preparation method and application of the probiotic composition. The probiotic composition is prepared from bacillus cecum, lactobacillus reuteri and an oligosaccharide composition; wherein the viable count of the bacillus cecum and the lactobacillus reuteri is 10 < 9 >-10 < 11 > CFU / g; the oligosaccharide composition is prepared by compounding polydextrose, fructo-oligosaccharide and galactooligosaccharide according to an optimized weight ratio. According to the invention, the oligosaccharide composition is cooperated with the probiotics to play a curative effect, so that the proliferation effect of the bacteroides cecum and the lactobacillus reuteri in the intestinal tract is improved, and the balance of the probiotics in the intestinal tract is regulated. Detection results of behavioristics and physiological indexes of mice show that the depression-like behaviors of the mice are remarkably reduced under the action of the composition, and various biochemical indexes are remarkably improved; the composition has a certain improvement effect on depressive mood.

The invention relates to a beta-galactosidase mutant with improved thermal stability and pH stability, and belongs to the technical field of enzyme engineering. The beta-galactosidase derived from Kluyveromyces lactis is subjected to molecular modification, a potential mutation site is determined by using an alanine scanning strategy, beta-galactosidase mutants Pro433Ala and Asp441Ala of which the thermal stability and the pH stability are simultaneously improved are obtained through site-directed mutagenesis screening, the catalytic efficiency of the mutants is improved to 1.03-1.18 times compared with that of the mutants before optimization, and the beta-galactosidase mutant can be used for preparing beta-galactosidase. Wherein the maximum half-life period of the mutant Asp441Ala under the condition of 50 DEG C reaches 91.5 minutes and is increased by 213.4% compared with that of a wild type, the problems of poor catalytic thermal stability and poor pH stability of beta-galactosidase in the prior art are solved, and a brand-new tool is provided for obtaining galactooligosaccharide by using beta-galactosidase to hydrolyze lactose.

The invention relates to the technical field of pharmaceutical compositions, and particularly discloses a proteolipid composite supplement with synergistic hangover alleviating and liver protecting functions and a preparation method thereof. The health-care food comprises the following components in percentage by mass: 10-25% of a Pu'er tea extract, 15-30% of glucose syrup, 5-15% of a hovenia acerba extract, 5-15% of a radix puerariae extract, 8-20% of corn peptide, 5-15% of oyster peptide, 3-10% of earthworm peptide, 1-5% of malic acid, 2-8% of multivitamins, 1-5% of an electrolyte compound, 3-10% of prebiotic fibers and 0.5-3% of gamma-aminobutyric acid. The compound vitamins comprise B1, B2, B6, B12, folic acid and vitamin C; the electrolyte compound is a nanoscale electrolyte compound and is an organic chelate containing potassium, magnesium and zinc; the prebiotic fiber is prepared by compounding galactooligosaccharide and resistant dextrin; through compounding design of active peptide components, plant active ingredients and functional factors, the active peptide improves the activity of key enzymes in ethanol metabolism in a targeted manner, and alcohol decomposition is accelerated.

The invention relates to the technical field of dairy product processing, in particular to high-protein native-dietary-fiber-rich zero-lactose milk and a preparation method thereof.The method comprises the following steps that 1, raw milk is treated through a reverse osmosis membrane, and RO penetrating fluid and RO retention fluid are obtained; 2) adding a proper amount of No.1 lactase into the RO retention solution, and hydrolyzing until the lactose concentration is less than or equal to 2g / 100mL and the galactooligosaccharide concentration is more than or equal to 3g / 100mL; 3) pasteurizing the hydrolyzed product, and inactivating the No.1 lactase to obtain a semi-finished product milk liquid containing the native dietary fibers; and 4) adding a proper amount of No.2 lactase into the semi-finished product milk liquid, and hydrolyzing until the lactose concentration is less than 0.5 g / 100mL to obtain the high-protein raw milk rich in native dietary fiber and zero in lactose. According to the method, lactase is subjected to enzymolysis on the premise that the sweetness of milk is not improved, galactooligosaccharide (dietary fiber) is generated in a natural enzymolysis mode, and the natural nutritional value of the product is improved.

The invention discloses zero-lactose milk containing galactooligosaccharide and a preparation method of the zero-lactose milk, and belongs to the technical field of dairy products. By means of the synergistic effect of the immobilized lactase and the acerola cherry powder extract, efficient hydrolysis of lactose is achieved, the lactose content is reduced to 0.1-0.5 g / 100 mL, meanwhile, 1.0-2.5 g / 100 mL of galactooligosaccharide is generated, and the lactase is especially suitable for people with lactose intolerance to drink. The preparation method comprises the following steps: pasteurizing the raw milk at 60-65 DEG C for 20-40 minutes, and then cooling; adding 0.01 to 0.3 percent of immobilized lactase and 0.001 to 0.1 percent of acerola cherry powder extract under the condition of 20 to 50 DEG C, and stirring and reacting for 10 to 60 minutes; then heating at the temperature of 80-120 DEG C for 5-20 minutes for enzyme deactivation; performing ultrasonic homogenization (200 to 500W, 20 to 70kHz, 10min), cooling and filling; and finally, carrying out ultrahigh pressure sterilization for 5 minutes at 500 MPa and 28 DEG C to ensure that the product is sterile. The stability and the repeated utilization rate of the enzyme are improved through the immobilized enzyme technology, and due to the addition of the acerola cherry powder extract, the product is endowed with natural oxidation resistance while the lactose is synergistically hydrolyzed.

The invention relates to the technical field of fermented fruit and vegetable juice, in particular to a compound probiotic fermented fruit and vegetable juice beverage and a preparation method thereof, and the compound probiotic fermented fruit and vegetable juice beverage comprises the following raw materials: apple juice, snow pear juice, carrot juice, a momordica grosvenori extracting solution, compound probiotics, zinc gluconate, citric acid, oat beta-glucan and nano diatomite loaded prebiotics. Nano diatomite is treated through acid pickling and 3-aminopropyltriethoxysilane, then a functional layer with gradient hydrophilic-hydrophobic balance is formed through molecular self-assembly of quercetin (hydrophobic area) and lecithin (hydrophilic head group), the structure is kept stable in fermentation liquor, the hydrophobic end of quercetin is embedded into a probiotic cell membrane phospholipid layer, the hydrophilic end of quercetin is exposed to a liquid phase, and the hydrophobic end of quercetin is embedded into a probiotic cell membrane phospholipid layer. The interface affinity is enhanced; and carboxylated galactooligosaccharide is further grafted, so that the feed has the directional nutritional characteristic of preferential utilization of probiotics.

A use of a nutritional composition in promoting the bidirectional development of the gut and brain via the gut-brain axis. The nutritional composition comprises the following essential components: docosahexaenoic acid, eicosatetraenoic acid, galactooligosaccharide, 1,3-dioleic acid-2-palmitic acid triglyceride, lutein, nucleotide, lactoferrin, casein phosphopeptide, and probiotics. By combining and strengthening multiple functional active components, the gut health and brain cognitive development of the organism can be bidirectionally promoted by means of the microbiota-gut-brain axis. In particular, the present application can promote the regulation of flora in the gut of the organism, increase the abundance of beneficial bacteria, and decrease the abundance of harmful bacteria related to brain development diseases. The content of butyric acid in the gut can be increased, and the contents of acetic acid and propionic acid are decreased to promote brain and cognitive development.

The present invention provides a nutritional composition comprising one or more of vitamin D, polydextrose and galacto-oligosaccharides in combination with a calcium source. The composition has the effect of improving the intestinal mucosal barrier including improving intestinal mucosal permeability, modulating expression of intercellular tight junction proteins, improving intestinal mucosal tissue structure and improving levels of markers of the intestinal mucosal barrier.

Methods and compositions use a galactooligosaccharide (GOS) for increasing absorption of zinc, such as zinc naturally present in ingredients of the composition and / or present in a food product, a meal or a diet, wherein the food product, meal or diet is combined with the composition and / or consumed separately from the composition. Some embodiments are directed to a milk-based composition comprising zinc intrinsically present in the milk, further comprising milk protein, and further comprising a mixture of lactose, glucose, galactose and the galactooligosaccharide (GOS). In some embodiments, the milk-based composition does not contain exogeneous zinc, such that the only zinc in the composition is the zinc intrinsically present in the milk.

The invention belongs to the technical field of biomass conversion and functional food ingredients, and discloses a method for preparing galactooligosaccharide prebiotics from cellulose. The method comprises the following steps: mixing cellulose with a galactose aqueous solution, and drying to obtain galactose pre-impregnated cellulose; impregnating the cellulose pre-impregnated with galactose with an acid catalyst solution, and drying to obtain cellulose impregnated with galactose and an acid catalyst; carrying out solvent-free ball milling treatment on the cellulose impregnated with the galactose and the acid catalyst, so that the cellulose is synchronously subjected to depolymerization and grafting reaction with the galactose to obtain a crude product; and carrying out purification treatment on the crude product, and removing the residual acid catalyst to obtain the galactooligosaccharide prebiotics. According to the galactooligosaccharide prepared by the preparation method disclosed by the invention, the water solubility of the oligosaccharide is greatly improved due to the introduction of a galactose branched chain, the yield of a final product is high, and the prebiotic activity of the final product is equivalent to that of a high-cost enzymic method commercial product and even is better for a specific strain.

The invention provides high-activity crataegus pinnatifida fruit vinegar and a preparation method thereof.The preparation method comprises the steps that crataegus pinnatifida fruits are juiced, then a composite chelating agent composed of polyglucuronic acid and monopotassium phosphate is added for chelating treatment, and a polysaccharide-metal ion cross-linked network is removed; then adding endo-beta-1, 4-glucanase to carry out linear polysaccharide directional enzymolysis, so that the viscosity of the fruit juice is obviously reduced; after the components are adjusted, double modified yeast beta-glucan microspheres are added, yeast is inoculated for alcoholic fermentation, the microspheres are subjected to double grafting modification through galactooligosaccharide and theaflavin, and active components in the crabapple can be directionally captured and stabilized through hydrophilic and hydrophobic effects respectively; after alcohol fermentation is finished, acetic bacteria are inoculated for acetic fermentation, and finally, filtering and sterilization are performed. According to the method disclosed by the invention, by virtue of a synergistic strategy of breaking a network firstly, then carrying out enzymolysis debonding and then carrying out microsphere loading, the fermentation mass transfer efficiency is effectively improved, and meanwhile, the retention rate of functional components such as polyphenol and saponin in the fruit vinegar is increased.

The invention relates to the use of beta 1,3′-galactosyllactose for ameliorating the harmful effects of food contaminants on the adaptive immune response such as vaccination response or response to a pathogen.

The present application relates to a kind of brain health nutrition capsule and its preparation method, belong to the technical field of functional health food.The preparation method includes the following steps: taking walnut kernel powder, DHA algal oil microcapsule powder, phosphatidylserine, ginkgo biloba extract and oligosaccharides are mixed, soft material is prepared by adding purified water, granulation is extruded by screen, and granule core is obtained by drying;Granule core is coated, and brain nutrition core granule is obtained by drying;Prepare base nutrition layer granule;Brain nutrition core granule and base nutrition layer granule are mixed, and composite granule is obtained, and composite granule is filled into capsule.The present application not only solves the three major obstacles of oxidation, precipitation and vitamin degradation of high polyphenol walnut base formula in gastric acid environment, but also unexpectedly builds from stomach protection to intestinal microecological optimization, finally realizes the cascade effect of brain nutrition efficient absorption, meets the core needs of teenagers brain health and intelligence, and truly realizes the effect of brain health and intelligence.

The invention relates to a solid beverage for maintaining intestinal-brain axes as well as a preparation method and application of the solid beverage, and belongs to the technical field of food processing. The health-care tea powder consists of cold-extracted freeze-dried tea powder, theanine powder, fructus phyllanthi powder, PQQ powder or microencapsulated PQQ powder, targeted probiotic microcapsule powder, mogroside and galactooligosaccharide. During preparation, the cold-extracted freeze-dried tea powder, the microencapsulated PQQ powder and the purchased targeted probiotic microcapsule powder are respectively prepared and then are mixed with other raw materials. The innovation of the invention lies in that the intestine-brain axis is synergistically maintained through a four-dimensional mechanism of anti-inflammatory and anti-oxidation-neuromodulation-mitochondrial activation-flora intervention, and the activity of the components is guaranteed by adopting technologies of cold extraction freeze-drying, microencapsulation and the like. The system can inhibit enteropathogenic bacteria, repair intestinal barriers, protect nervous systems, improve cognitive functions and regulate intestinal flora, has high technical feasibility and market prospects, and makes remarkable progress compared with the prior art.

The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus rhamnosus FMBL L25209 MQ with the effects of resisting oxidation and prolonging the service life, the lactobacillus rhamnosus FMBL L25209 MQ is preserved in the China Center for Type Culture Collection on September 18, 2025, the preservation number is CCTCC M 20252059, and the lactobacillus rhamnosus FMBL L25209 MQ can utilize oligosaccharides such as galactooligosaccharides, soybean oligosaccharides and maltooligosaccharides; good tolerance to acid and cholate is achieved; the compound has the effects of inhibiting bacteria, resisting oxidation, prolonging the service life, resisting aging and the like, can be used for preparing medicines for resisting oxidation, resisting aging and prolonging the service life, and has a wide application prospect.

The invention discloses a process for preparing a rice protein-prebiotic compound based on Maillard reaction, and belongs to the technical field of preparation of food additives and functional components. The process comprises the following steps: dissolving rice protein and prebiotics (such as fructo-oligosaccharide, galactooligosaccharide, inulin and the like) in water according to a specific mass ratio, adjusting the pH value to be alkaline, and treating through an efficient wet ball milling technology to obtain a nanoscale composite solution; then controllable Maillard reaction is carried out, and the rice protein-prebiotic covalent complex with high grafting degree and moderate browning degree is prepared by adjusting the reaction temperature, time and humidity. The compound has the excellent amino acid composition of the rice protein and the physiological activity of prebiotics, the solubility, emulsibility and stability of the rice protein are remarkably improved, and meanwhile, the prebiotics function of promoting intestinal health is given to the compound. The method is mild in process condition, green and efficient, and the obtained product can be widely applied to the fields of functional foods, special medical foods, dietary supplements and the like.

The present disclosure relates generally to spray-dried milk or milk-based powder compositions comprising galactooligosaccharides (GOS) and low levels of lactose, and methods of making the compositions.

Methods and compositions improve immune health. A method for improving immune health comprises administering to an individual in need thereof a composition comprising milk protein, preferably from 20% to 40% by dry weight of the composition; and a mixture of lactose, glucose, galactose and galactooligosaccharide, preferably from 32% to 55% by dry weight of the composition. The mixture of lactose, glucose, galactose, and galactooligosaccharide may contain from 0.1% to 30% by weight of the lactose, from 8% to 40% by weight of the glucose, from 32% to 55% by dry weight of the glucose, from 0.1% to 20% by weight of the galactose, and from 40% to 90% by weight of the galactooligosaccharide.

The invention discloses targeted probiotic dietary powder for conditioning intestinal microecology and a preparation method of the targeted probiotic dietary powder. According to the invention, the micro-ecological health of intestinal tracts is obviously improved through a triple synergistic mechanism. The core probiotic combination directly acts on the key link of intestinal flora imbalance. Lactobacillus plantarum LP-12 inhibits excessive release of intestinal inflammatory factors and alleviates intestinal mucosa damage. The bifidobacterium bifidum TMC3115 enhances tight connection of intestinal epithelial cells and repairs a physical barrier; the Witzia ciliaris MKSWC01 can degrade endotoxin and block the chain reaction of chronic inflammation. Meanwhile, the fucoidin and the galactooligosaccharide are used for accurately feeding the probiotics and enhancing the colonization capability of the probiotics in a colon mucus layer, so that long-acting protection is formed. L-glutamine provides basic nutrition required by intestinal mucosa regeneration, and resistant dextrin continuously generates short-chain fatty acid to maintain a weak acid environment of colon, so that healthy flora ecology is comprehensively reconstructed.

The pet meat staple food grain based on composite prebiotic fermentation and the preparation method thereof have the advantages that 1, a composite prebiotic combination adopts a synergistic proportion of fructo-oligosaccharide, inulin, stachyose and galactooligosaccharide, and the regulation efficiency of intestinal flora is remarkably improved through the design that the proportion of a GF2 component is greater than or equal to 70% and the proportion of a long-chain component (the polymerization degree is 6-8) is greater than or equal to 20%; 2, animal meat sources (cattle tendon and chicken breast) and fermented plant raw materials are scientifically proportioned, and high-bioavailability protein and soluble fiber are provided; 3, the probiotic preparation is compounded by lactobacillus acidophilus and bifidobacterium in a ratio of 1: 1.5, and the survival rate of viable bacteria and the yield of metabolites are ensured in combination with a staged fermentation process; and 4, the mineral premix and the vitamin compound are accurately added, so that the nutritional requirements of pets in different life cycles are met, and the synergy of nutrition comprehensiveness and functionality is realized.

The application discloses a composition for improving bone density and application thereof, and belongs to the technical field of food. The composition is prepared by using 1,3-dioleic acid-2-palmitic acid triglyceride, osteonectin and oligosaccharide in combination, and through the interaction between the components, the absorption and utilization of each other are promoted, the growth and development of the bone of an infant in the growth period are improved, the bone mineral content and bone density are improved, the bone health is promoted, and the composition is safe and has no side effect. Through optimization of the types of the oligosaccharide, it is found that the combination of oligogalactose and 2'-fucosyllactose can better improve the growth and development of the bone of the infant in the growth period.

The application relates to the technical field of medicines or cosmetics, and particularly discloses a composition for eliminating acne and smoothing skin and a preparation method thereof, which is prepared from components including a prebiotic combination, a plant extract, a fermentation extract, a bioactive ingredient and a composite probiotic freeze-dried powder; the prebiotic combination contains raw materials of galactooligosaccharide and isomaltooligosaccharide; the plant extract contains raw materials of cherry powder; the fermentation extract is prepared from spilanthes acmella, houttuynia cordata and lily after probiotic fermentation; the bioactive ingredient contains raw materials of yeast zinc and yeast beta-glucan; the composite probiotic freeze-dried powder contains a probiotic powder composed of paracasei LY12, lactobacillus plantarum LP28 and lactobacillus rhamnosus LRH113, lactobacillus acidophilus LA1 and lactobacillus rhamnosus GG. The application solves the problems of poor acne treatment effect, easy recurrence and large side effect in the prior art, and finally realizes the effect of efficiently eliminating acne and smoothing skin.

The invention discloses a prebiotic composition rich in dendrobium officinale polysaccharides. The prebiotic composition comprises the following components in parts by weight: 10-30 parts of dendrobium officinale polysaccharides, 4-28 parts of xylooligosaccharide, 5-20 parts of stachyose, 10-30 parts of galactooligosaccharide, 4-20 parts of psyllium husk powder and 10-30 parts of taste adjusting powder. According to the prebiotic composition rich in the dendrobium officinale polysaccharide, the efficient delivery and stability of the dendrobium officinale polysaccharide are ensured through the careful selection of the raw materials and the collaborative design of the formula, and the intestinal health promotion function is improved. The invention further provides a tablet candy adopting the prebiotic composition and a preparation method of the tablet candy.

A method for producing a secreted β-galactosidase, characterized by integrating a non-secreted β-galactosidase gene derived from a basidiomycetous yeast into Aspergillus oryzae to produce a secreted β-galactosidase, and a method for producing a galactooligosaccharide using a β-galactosidase produced by the method facilitate the production of a galactooligosaccharide.

The invention provides a preparation method of a double-layer functionalized PS / GO nanofiber membrane, which can realize co-immobilization of yeast and beta-galactosidase, and belongs to the technical field of material chemistry and food biology. According to the method, beta-galactosidase and saccharomyces cerevisiae are successfully fixed on a functionalized graphene oxide modified double-layer polystyrene nanofiber membrane through a packaging method and an adsorption method, monosaccharide-free galactooligosaccharide (GOS) is obtained in one step by taking lactose as a raw material, and a novel biological catalysis system integrating GOS biosynthesis and monosaccharide removal is successfully constructed. In the catalytic system, the hydrolysis activity of the immobilized beta-galactosidase and the transglycosidase activity are remarkably improved compared with free enzymes, and the synthesis efficiency of GOS is effectively improved through the high transglycosidase activity of the immobilized beta-galactosidase. After being treated by the immobilized yeast cells, the yield of the GOS is still kept at a relatively high level of 56%, and more than 90% of monosaccharide in the product is consumed by the yeast cells, so that the high-purity GOS is obtained. And the catalytic system can be repeatedly used for multiple times, and the beta-galactosidase still keeps the enzyme activity of 50% or more after being recycled for 20 times, so that the preparation and production cost of the GOS can be effectively reduced. According to the preparation method of the double-layer functionalized PS / GO nanofiber membrane and the co-immobilization method of the yeast and the beta-galactosidase, provided by the invention, high-efficiency and low-cost preparation of high-purity GOS can be realized.