The invention relates to a determination method for the content of
carotenoid in wheat. The determination method comprises the following steps: (1) preparing a standard sample, namely mixing xanthophylls,
zeaxanthin, alpha-
carotene and beta-
carotene standard substances to prepare a series of
standard samples; (2) preparing a to-be-detected solution, namely, dissolving extracted
carotenoid into a
mixed solution of methyl
alcohol and
ethyl acetate with the ratio of 68 to 32 to obtain a
mixed solution, centrifuging the
mixed solution to obtain a centrifuging solution, and enabling the centrifuging solution to pass through an oily filter membrane so as to obtain the to-be-tested solution; (3) testing by adopting an ultra-
high performance liquid chromatography, namely testing under a defined chromatographic condition; and (4)
processing testing data, namely, drawing a
standard curve, and substituting tested
peak area data of the to-be-tested solution into the
standard curve so as to obtain the content of each component of the
carotenoid in the wheat. According to the determination method, the time spent on extracting the carotenoid in the wheat is short, and the efficiency is high; the adopted ultra-
high performance liquid chromatography has the characteristics of rapidness, high efficiency, high analysis flexibility and the like, and the xanthophylls and the
zeaxanthin can be separated completely; the determination method can be widely applied to the determination of the content of the carotenoid in the wheat.