96 results about "Dunaliella" patented technology

The invention discloses a method for producing a forage immune enhancer by adopting a soybean curb residue and an orange peel. The method comprises the following steps: respectively crushing the soybean curb residue, dried pine needles, dry dunaliella cells and dry orange peel into coarse powder of 60-80 meshes; burdening the raw materials in parts by weight: 45-50 parts of soybean curb residue, 30-35 parts of dried pine needles, 2-5 parts of dry dunaliella cells, 15-18 parts of dry orange peel and 2.0-2.5 parts of an assistant, wherein the assistant is prepared from 49 parts of NaHCO3 and 1 part of MgSO4; the moisture content of the pine needles, the dunaliella cells and the orange peel is 3-5%, the moisture content of bean dregs is 18-20%; mixing the mixture of four coarse powder with the assistant according to the ratio, wherein the assistant accounts for 2.0-2.5% of dry weight of the total material; evenly mixing and then grinding for 30 minutes; filtering by a 400-mesh sieve; processing by using enzyme-aid micronization treatment, so as to obtain the forage immune enhancer product, wherein the treatment time is 15-30 minutes according to the quality and the humidity of the raw materials. By adopting the forage immune enhancer produced by the method disclosed by the invention, the production cost of the raw materials is significantly reduced, a lot of waste bean dregs produced in bean product production are recycled, low-carbon production is achieved, and the added value of products is increased.

Methods for controlling a density of algae growing in an aquatic environment are provided. Exemplary methods include applying an effective amount of glyphosate to a density of algae growing in an aquatic environment. The algae may include genus Nannochloropsis and/or Dunaliella. The algae may also include a glyphosate resistant strain of genus Nannochloropsis. The effective amount may result in an approximate concentration of between 0.1 millimolar to 0.3 millimolar glyphosate in the aquatic environment. Additionally, the aquatic environment may include seawater. The glyphosate may be applied to the aquatic environment before and/or after the aquatic environment is inoculated with algae. Alternative methods include applying an effective amount of glufosinate to a density of algae growing in an aquatic environment.

The invention relates to the technical field of aquaculture feedstuff, in particular to feedstuff additive premix material. The feedstuff additive comprises the following components according to the following weight portion: 150 to 450 portions of astaxanthin, 300 to 700 portions of dunaliella sallina, 150 to 400 portions of vitamin E, 300 to 600 portions of Chinese thorowax, 100 to 250 portions of canthaxanthin, 300 to 600 portions of antioxidant, and 150 to 1,000 portions of corn protein powder. The feedstuff additive has no innocuous and side residue, no drug resistance, environmental protection, and no adverse effect on the feedstuff palatability. The feedstuff additive can obviously improve the body color of fishes in the yellow and red color systems such as golden pompano, pseudobagrus fulvidraco, clarias fuscus, sea bream, large yellow croaker, and the like, can make the body color display natural yellow or red color, and can effectively improve the immunity, reproductive capacity and meat quality of cultivated fish. In addition, the feedstuff additive has stable raw material sources and a simple manufacturing process.

The invention belongs to the technical field of soil remediation, and in particular relates to a heavy metal polluted soil remediation agent. Soil remediation agent contaminated by heavy metals, including the following components: chitosan, bentonite, Dunaliella, citric acid, shell powder, enoki mushroom chaff, corn cob, rice bran, tea seed hulls, moss aspergillus, betel nut wood chips , Palm wood chips, compound enzyme preparation, compound microbial agent. The soil remediation agent of the invention is used to treat the soil with mild enzymes and microbial strains without introducing other chemical solvents, without introducing new pollution, and the action conditions are mild.

The invention relates to a high-density culture process of autotrophic oil-producing microalgae. A fermentation method combining a partition method and a two-step method is adopted, CO2 waste gas discharged from a thermal power plant is used as a carbon source, municipal sewage after living contaminant or miscellaneous algae filtering removal is used as a culture medium, the autotrophic oil-producing microalgae is cultured in a high-density way for growth, perfectly, sea salt is added into the municipal sewage for simulating sea water so that the permeation pressure is increased, the living contaminant or miscellaneous algae pollution is prevented, and the autotrophic oil-producing microalgae is selected from one kind or several kinds of materials from autotrophic chlorella, nannochloropsis salina, botryococcus braunii, chaetoceros gracile, marine green algae, isochrysis galbana, nitzschia closterium, phaeodactylum tricornutum, dunaliella and prism-shaped algae. When the high-density culture process is adopted, high removal efficiency of nitrogen, phosphorus and CO2 can be realized, in addition, the algae concentration, the grease content and the protein content are high, the double effects of environment protection and biological resources production are reached, the operation is simple, convenient and fast, the cost is low, no pollution is caused, social and economic integrated benefits are high, and the high-density culture process is suitable for large-scale popularization and application.

The invention belongs to the technical field of biological culture of algae, and relates to a dunaliella culture method. The dunaliella culture method comprises the following steps: at first, regulating the light intensity and temperature in a dunaliella culture reactor to adapt to dunaliella propagation, inoculating dunaliella into a dunaliella culture agent containing a salt, a nitrogen source (KNO3), a phosphorus source (KH2PO4) and an inorganic carbon source (NaHCO4), respectively measuring the nitrogen source, the phosphorus source and the inorganic carbon source in the dunaliella culture agent after a growth period, a logarithm growth period and a platform period of dunaliella culture, making the nitrogen source, the phosphorus source and the inorganic carbon source respectively reach set values, and measuring dunaliella biomass to complete the dunaliella propagation; regulating the light intensity and the temperature to adapt to beta-carotene accumulation, respectively adding table salt, KNO3, KH2PO4 and NaHCO4 into the dunaliella culture agent to set values, culturing until the dunaliella becomes brown, and then the beta-carotene is accumulated and the dunaliella culture is completed. The dunaliella culture method is scientific and reasonable in culture process, simple in steps, stable in performance, free of external environment influence and high in product utilization rate.

The invention relates to a method for controlling microbe indexes in microalgae powder by combination of pasteurization and spray drying and a method for producing microalgae powder. The method comprises the following steps: pasteurizing a microalgae concentrated solution to obtain a sterilized concentrated solution, and drying the sterilized concentrated solution to obtain microalgae powder, thereby controlling the microbe indexes in the microalgae powder (including but not limited to total bacterial count, mold quantity and yeast quantity); and producing the microalgae powder. The method can effectively control the microbe indexes (especially total bacterial count) of the algae powder, and can maximally maintain the activity of the active substances in the microalgae. The method is suitable for but not limited to producing Chlorella, Haematococcus pluvialis, spirulina, Dunaliella, Nannochloropsis oculata, Schizochytrium, Crypthecodinium cohnii and various algae powders, and is hopeful to become a universal production method capable of effectively controlling microbe indexes (especially total bacterial count) in the algae powder.

The invention discloses an organic fertilizer and a preparation method thereof. The organic fertilizer comprises the following raw materials by weight: 12 to 16 parts of blue algae, 6 to 8 parts of diatomite, 20 to 22 parts of dunaliella, 15 to 17 parts of spinaciaoleracea, 12 to 14 parts of eucheuma, 6 to 8 parts of rice washing water, 14 to 16 parts of wormcast, 8 to 10 parts of apple snail, 25 to 27 parts of poultry dung, 12 to 16 parts of cottonseed meal, 7 to 9 parts of zoysia japonica, 12 to 16 parts of sudan grass, 13 to 15 parts of raphanussativus L., 18 to 20 parts of swill, 4 to 6 parts of an EM stock solution, and 18 to 20 parts of rauhkalk. The organic fertilizer is sufficient in fertility and high in efficiency.

The invention belongs to the technical field of soil restoration and particularly relates to a heavy metal contaminated soil restoration material. The heavy metal contaminated soil restoration material is composed of chitosan, bentonite, Dunaliella, oxalic acid, shell powder, flammulina velutipes fermented bran, corncobs, rice bran, tea seed shells, dicranum scoparium, areca wood chips, palm wood chips, a compound enzyme preparation and a compound microorganism agent. By the adoption of the soil restoration material, mild enzymes and microorganism strains are adopted for treating soil under the condition of not introducing other chemical solvents, new contamination is not brought in, and action conditions are mild.

The invention relates to a method for controlling microbe indexes in microalgae powder by combination of microwave sterilization and spray drying and a method for producing microalgae powder. The method comprises the following steps: carrying out microwave sterilization on a microalgae concentrated solution to obtain a sterilized concentrated solution, and drying the sterilized concentrated solution to obtain microalgae powder, thereby controlling the microbe indexes in the microalgae powder (including but not limited to total bacterial count, mold quantity and yeast quantity); and producing the microalgae powder. The method can effectively control the microbe indexes (especially total bacterial count) of the algae powder, and can maximally maintain the biological activity of the active substances in the microalgae. The method is suitable for but not limited to producing Chlorella, Haematococcus pluvialis, spirulina, Dunaliella, Nannochloropsis oculata, Schizochytrium, Crypthecodinium cohnii and various algae powders, and is hopeful to become a universal production method capable of effectively controlling microbe indexes (especially total bacterial count) in the algae powder.

The invention relates to the field of aquiculture feed, in particular to a high-efficiency compound feed for young abalone. The high-efficiency compound feed is characterized by consisting of fish meal, soybean protein concentrate, casein, beer yeast, spiral seaweed, dunaliella, undaria, artemia, monocalcium phosphate, lecithin, choline chloride and abalone premix compound. The high-efficiency compound feed has the advantages that (1) all raw materials of the compound feed are stable in source and complete in nutrition constituents, so that all nutrition requirements required by growing the young abalone can be met; (2) each component of the feed is balanced in collocation, so that the high-efficiency compound feed is good for the young abalone to digest and assimilate in a balanced way, is convert in growth, is small in the organic detritus of the excreta, and has less influence to the substrate; and (3) the immunity and the anti-stress capability of the young abalone can be obviously increased, and the young abalone is more vigorous.

The invention relates to a culture method of Dunaliella and application of Dunaliella in biomass energy, which relate to the Dunaliella. The invention provides a culture method of Dunaliella which is simple and economical, not only can be used as the biomass energy, but also can efficiently capture and store carbon dioxide, and the application of the Dunaliella in the biomass energy. The culture method is characterized by filtering in sea water; inoculating the dunaliella seed in sea water culture base, culturing the seed for 14 to 35 days, mixing continuously every day, culturing the dunaliella for a year, and harvesting the dunaliella cell when density of the dunaliella reaches 104 to 106 per ml. The dunaliella is used as a material of the biomass energy, high-concentration sea water isused as culture base, and the carbon dioxide is assimilated through the growth of the dunaliella so as to accumulate the biomass energy materials such as grease, starch and the like.

Preparation and application of a fused oral interferon expression frond for fowl are disclosed. On the basis of the conserved sequences of fowl interferon alpha and gamma genes, the interferon alpha gene having great antiviral effect and the interferon gamma gene capable of improving the immunity of the organism and enhancing the constitution of the organism are fused for expression, so that the expressed alpha+gamma fused interferon has the synergetic functions of the two. In the mean time, a dunaliella bioreactor is used as an expression system; apart from that the system is capable of expressing exogenous genes, own advantages such as rich nutrition, nontoxicity, harmlessness and the like of dunaliella are also utilized; consequently, a gene algae plant for multiple purposes is obtained; the fused oral interferon expression frond for fowl prepared from the genetically modified algae plant can be applied to the fields such as prevention or treatment of fowl viroses, fowl breeding, related feed processing industry and the like; and the fused oral interferon for fowl is good in effect, green and environment-friendly, and also low in cost.

The invention discloses a method for preparing an oral avian influenza vaccine from transgenic dunaliella. The method particularly comprises the following steps of: introducing a controlling element of the dunaliella, constructing a double-component expression vector which contains recombination genes of avian influenza viruses, namely, HA and NA, transforming the dunaliella and screening and identifying so as to determine a dunaliella transformation strain of a high-expression recombinant C3d-H5N1 fusion protein; and amplifying a stable expression C3d-H5N1 fusion protein transformation strain, performing large-scale open culturing on the strain, centrifugally collecting frustules, refrigerating, drying and packaging under an aseptic condition so as to prepare oral avian influenza vaccine capsules. The dunaliella transformation strain prepared by the method can be cultured in an open state, so that high yield and low cost are realized. Therefore, mass production of the oral animal avian influenza vaccine prepared from the transgenic dunaliella is realized and market demand is met.

The invention relates to a method for biologically synthesizing lycopene, in particular to a method for enabling Dunaliella to accumulate high-content lycopene. The method comprises the following steps of: extracting Dunaliella genome DNA; cloning a lycopene-beta-cyclase (LycB) gene of the DNA; constructing an LycB gene knockout vector and transforming the vector into a Dunaliella salina cell; and screening and identifying to obtain LycB gene knockout Dunaliella, wherein the LycB gene expression of the Dunaliella is inhibited; the process of biologically synthesizing beta-carotene by using the cell is stopped at the lycopene stage; and under the stress condition, a large amount of lycopene can be accumulated in an organism and the lycopene content is 3 to 4 percent of the dry weight of the Dunaliella. The method is a new way of obtaining a large amount of natural lycopene with low price.

The ultraviolet ray mutagenesis, selective breeding and identification method of cold tolerant Dunaliella includes: inoculating Dunaliella to culture medium; lighting and dark inducing for synchronized growth; mixing Dunaliella liquid with iodine solution or bromophenol blue solution to deactivate Dunaliella, injecting Dunaliella liquid to culture dish, mutagenesis under ultraviolet lamp before dark culturing, mixing with fresh culture liquid and painting the mixture to Dunaliella culture medium for low temperature lighting culture; inoculating single Dunaliella colony to the culture liquid and low temperature lighting culturing; sampling detection to comparing the low temperature lighting growth curves of cold tolerant Dunaliella mutant and wild prototype strain; extracting total DNA for RAPD comparison; calculating genetic similarity coefficient; extracting total protein and post-electrophoresis staining while record results; comparing protein electropherogram; and confirming the obtained cold tolerant Dunaliella mutant.

There are disclosed a composition containing 9-cis beta -carotene in high-purity and an industrially applicable method of obtaining the same from an alga. The composition can be obtained through steps of washing with ethanol dry powder of the alga belonging to Dunaliella, adding hexane to the washed algal powder, stirring the same, subjecting the same to filtration to obtain a filtrate, concentrating the filtrate to obtain a semi-solidic concentrate, adding hexane to the semi-solidic concentrate in an amount of 10-18 ml of hexane to 1 g of the semi-solidic concentrate, stirring the same, subjecting the same to filtration to obtain a filtrate, concentrating the filtrate to obtain an oily concentrate, adding hexane to the oily concentrate in an amount of 2-4.5 ml of hexane to 1 g of the oily concentrate to dissolve the same, and leaving the solution, as it is, under lower temperature condition to cause a separation of the composition. The composition is a rubiginous powder or crystals and contains 9-cis beta -carotene in an amount of 75% or more to total amount of the composition.

The invention discloses a composition for promoting meiotic resumption of oocytes of sows and application of the composition. The composition comprises vectors and extracts of ajuga forrestii, oregano, horseradish, cinnamon, rosemary, dunaliella and rhoeo discolor. The composition and the application have the advantages that meiotic resumption of the oocytes of the sows can be promoted, the quality of the oocytes can be improved, the reproductive performance of the sows can be improved, the reproductive lives of the sows can be prolonged, the composition is favorable for activating c-mos and AMPK (adenosine monophosphate activated protein kinase), accordingly, starting of the meiotic resumption of the oocytes of the sows can be promoted, the quality of the oocytes of the sows can be improved, the conception rates and the pregnancy proportions of the sows can be increased, the reproductive health of the sows can be promoted, the immunity and health conditions of the sows can be improved, and the service lives of the sows can be prolonged; the composition can be added into feed for the sows, so that the quantities of eliminated sows among reservation sows and adult sows due to poor oocyte quality of the sows can be greatly reduced, the sow elimination rate can be lowered, and the service lives of high-parity sows can be prolonged; the costs of pig farms can be reduced, and the productivity of the pig farms can be improved.

The invention discloses a method for extracting chlorophyll from dunaliella by using inorganic alkali, which comprises the following steps: (1) cooling and freezing dunaliella mud or dunaliella powder added with a proper amount of water, unfreezing in a water bath till ice crystals are eliminated; (2) regulating pH value by using aqueous solution of an inorganic alkali, and extracting for 3 to 5 hours at 60 to 70 DEG C; (3) centrifuging the extract solution and collecting supernate; (4) regulating the pH value of the supernate by using an inorganic acid to precipitate chlorophyll; and (5) centrifuging, removing supernate to obtain chlorophyll and freezing and drying the chlorophyll. The method disclosed by the invention overcomes the drawback of organic solvent residue of the product obtained by an organic solvent extraction method and the drawbacks of big investment and high economic cost of a supercritical extraction. When the method is used, a convenience is provided for chlorophyll extraction and the trouble brought by the need of removing acetone, petroleum ether and other organic solvents in a subsequent polysaccharide extraction process is avoided.

The invention belongs to the field of wastewater treatment, and provides a resource utilization method of livestock excrement water-soluble manure production wastewater for culturing dunaliella. The method comprises the following steps: 1) diluting livestock excrement water-soluble manure production wastewater with water, 2) adding nutrient elements like P, Ca, Fe and Mg into the diluted livestock excrement water-soluble manure production wastewater, 3) using one or more of a NaCl solution, seawater and brine to adjust the salinity value of the wastewater to be 150-200, and controlling the pH value to be 8.0-9.0, (4) vaccinating dunaliella in the logarithmic growth phase for culture, and 5) continuously culturing for 8-12 d so as to obtain dunaliella after dunaliella grows to be in the platform phase. According to the provided method, treatment of the livestock excrement water-soluble manure production wastewater and recycle of the nutrient elements are combined, pollutants in the wastewater can be desorbed, biomass raw materials can be produced, and multiple efficacies of environmental protection, cost saving, resource recycle and nutrient element cyclic utilization are played.

The invention relate to a cDNA sequence of glycerol-3-phosphate dehydrogenase genes DvGPH1 and DvGPDH2 associated with glycerol synthesis, and an amino acid sequence of an encoding protein. The expression vector of the DvGPH1 or DvGPDH2 gene is translated into a yeast gpd1delta cell. The yeast cell shows a salt resistant phenotype on a culture medium containing salts, thereby verifying that the two genes can strengthen glycerol synthesis in the yeast cell and improve the salt resistance of the yeast cell, and simultaneously verifying that the two genes have good application value on improving the glycerol synthesis amount in organisms such as the yeast through genetic engineering means and strengthening the salt resistance of the organisms. As the invention further improves the glycerol-3-phosphate dehydrogenase activity and the capability of catalyzing and synthesizing the glycerol of the two gene products and the salt resistance of a corresponding yeast transformant through the deletion of part of sequences of the DvGPH1 or DvGPDH2 genes, the two deletion type gene derivatives have better prospect in the application fields. In addition, the invention verifies that the expression of the DvGPH1 and DvGPDH2 genes in a dunaliella is subjected to the induction of salt stress, thereby showing that the two genes are the key enzymes for dunaliella synthetic glycerol and further providing the theoretical base for the application of the two genes to the fields.

The invention discloses a dunaliella culture medium utilizing sodium hydrogen carbonate as a carbon source and application thereof, and particularly discloses a dunaliella culture medium utilizing hydrocarbonate as a unique carbon source and a method for culturing dunaliella by utilizing the dunaliella culture medium. The optimization of the culture medium comprises the optimization of calcium andmagnesium ions and the optimization of trace elements. By adopting the dunaliella culture medium provided by the invention, the source of the carbon source used in the dunaliella can be quite well provided; thus, the growth of the dunaliella is ensured; meanwhile, by utilizing the sodium hydrogen carbonate as the carbon source, the accumulation of beta-carotene in dunaliella salina can be also improved; more importantly, by utilizing the hydrocarbonate as the carbon source, the pH (potential of Hydrogen) in a culture system can be also maintained in the range beneficial to the growth of the dunaliella salina, so as to improve the growth speed of the dunaliella salina; however, the pH is not so high as to cause the death of the cultured dunaliella salina; the culture method of the dunaliella is simple; the cost is low; the utilization efficiency of carbon is high, and the dunaliella culture medium is suitable for industrialized application.

The invention relates to a glutamine synthetase gene, a protein coded by same and a cloning method thereof. The gene is from dunaliella, the base sequence of the gene is represented by SEQ ID No.1, and the gene has a function of synthesizing glutamine synthetase and is proved to be capable of obviously improve the glutamine synthesizing capacity of Escherichia coli which is a model organism. Thus, it is indicated that the disclosed full-length gene and the amino acid sequence of the gene have application values in plant gene engineering aspects of improving nitrogen utilization rate of organisms and increasing nitrogen content in organisms.

A method for treating a disease selected from diabetes mellitus and atherosclerosis, and a method for reducing triglycerides and/or increasing HDL cholesterol levels in the plasma of a subject. The method comprises administrating. to a subject an effective amount of crude Dunaliella powder, optionally together with an activator of nuclear receptors.