29 results about "Model organism" patented technology

The invention discloses construction and application of a confinement enhanced photoelectrochemistry-electrochemiluminescence dual-mode biosensor, and relates to the technical field of biosensing and analytical chemistry. The preparation method of the biosensor comprises the following steps: taking a CdS (at) SiO2 (at) NaYF4: Yb / Tm nano composite material as a substrate to obtain a confinement enhanced photoelectrochemistry (PEC) and electrochemiluminescence (ECL) sensing interface; in combination with a DNA wheel nanostructure triggered by target acetamiprid, signal amplification is realized; and synchronous dual-mode quenching detection of PEC and ECL signals is realized by virtue of peroxidase-like catalytic precipitation and wide-spectrum absorption characteristics of the PtPd-CoSnO3 nanocube. The biosensor prepared by the invention has the advantages of high sensitivity, strong reliability, good selectivity and the like, can be applied to accurate detection of acetamiprid in samples such as agricultural products and the like, and has important application value in the aspects of environmental monitoring and food safety control.

In certain aspects, the invention provides methods and compositions for heritable immunization and their use in infectious disease control. The invention also provides methods of preparing organisms and model organisms that can heritably transfer immunization to offspring.

Methods and systems for automating surgical procedures in model organisms. The system includes a user input panel, a trained computer vision system, a server, and articulated robotic arm. The system may further include an analytical scale, an anesthesia chamber, a tube labeling system, a tissue freezing system, and a biohazard waste disposal system. The computer vision system communicates with the robotic arm to coordinate tissue collections and common research procedures such as injections via recognition models related to detection, identification, localization, and classification. The central server provides rapid synchronization of data between the local machine and a cloud account system for real-time data analytics. The anesthesia chamber provides standardized administration of anesthesia, the sample labeling system provides 2D barcoded labels according to user input, and the tissue freezing and waste disposal systems enable storage of samples and removal of carcasses following tissue collection, fully automating the necropsy.

The invention belongs to the crossing field of marine biotechnology and bioinformatics, and particularly relates to a method for high-throughput identification of natural functional oligopeptides. At present, bottlenecks still exist in development and application of various functional oligopeptides such as penetrating peptides and antibacterial peptides in non-model species, and the high adaptability of the existing functional oligopeptides in complex and diverse non-model organisms is limited mainly due to differences (amino acid preference and receptor specificity) among species. Therefore, the invention provides a method for high-throughput identification of functional oligopeptides from natural sequences of organisms by means of machine learning. According to the method, based on a biological natural protein sequence, an oligopeptide sequence set meeting a preset length condition is generated through a sliding window strategy system, a deep machine learning model is utilized to perform high-throughput functional prediction on the oligopeptide sequence, and on the basis of performing'interruption-prediction-re-comparison 'on existing functional oligopeptides, the functional oligopeptide sequence set meeting the preset length condition is obtained. The comparison threshold value for accurately identifying the functional oligopeptide can be determined, and the natural functional oligopeptide existing in the protein sequence can be effectively identified based on the threshold value. The functional oligopeptide obtained by the method has strict screening of physicochemical properties of a machine learning model and high-adaptability biological characteristics formed in species evolution. According to the method, the direct application capability of the functional oligopeptide in non-model species is remarkably improved, and an accurate and high-throughput technical means is provided for efficient development and application of the functional oligopeptide in non-model organisms.

The invention belongs to the technical field of medicines, and particularly relates to application of vitamin B12 in preparation of a medicine for treating retinitis pigmentosa. Specifically, based on the high conservative property of human PRPF31 and caenorhabditis elegans prp-31 genes, a prp-31 gene knock-down and mutation nematode disease model is constructed, and the model can reproduce multiple pathological phenotypes such as oxidative stress, lysosome dysfunction and apoptosis caused by PRPF31 defects. Experiments prove that the active form (such as mecobalamin) of the vitamin B12 can actively pass through the blood retina barrier by virtue of an endogenous transport mechanism through oral administration, the pathological damage can be reversed by virtue of multi-channel synergy, and the pathological phenotype of a model organism can be remarkably improved, so that the vitamin B12 has a good practical application value.

The present application relates to the technical field of water quality restoration, and more particularly to a multi-source sensing intelligent closed-loop water quality restoration system and a control method thereof, which is suitable for river management, landscape water maintenance, industrial wastewater treatment and the like, and is particularly suitable for small and medium-sized water body restoration projects that need to dynamically respond to water quality changes. By means of multi-channel online monitoring, physicochemical index data and model organism activity data are obtained, combined with artificial sampling data, an evaluation model is used to comprehensively evaluate the water quality and grade the abnormality level; and according to the abnormality level, a graded restoration program is automatically triggered, and automatic backflow, automatic sample reservation and remote alarm are simultaneously executed, so as to realize closed-loop, accurate and efficient restoration of water quality and whole-process traceable management, improve restoration efficiency and reliability, and reduce the frequency of manual intervention.

The invention belongs to the technical field of bioengineering and analytical chemistry, and provides a probe eZinCh-2 modified plasmid based on FRET (Fluorescence Resonance Energy Transfer) and application of the probe eZinCh-2 modified plasmid in zinc ion detection. According to the invention, the plasmid is constructed through molecular cloning and homologous recombination technologies, and is microinjected into a zebrafish embryo, so that a transgenic strain capable of stably inheriting and expressing the probe protein is successfully obtained; as a living body biological tool, the zebrafish of the strain is combined with a confocal microscope spectrum FRET technology, and real-time and quantitative monitoring and visual imaging of dynamic changes of zinc ions at living body, cell and tissue levels are achieved. The method solves the problem that real-time dynamic visual monitoring of the zinc ions cannot be realized in a living body model in the prior art, and is suitable for real-time microscopic quantitative analysis of the content of the zinc ions in model organisms such as zebra fish and the like.

The invention belongs to the field of biosensor preparation methods, and particularly relates to a PNA-based electrochemical-photo-thermal dual-mode biosensor and a method for detecting an SRB DsrC gene. An electrode structure of the sensor is CHA / PNA / ITO, a PNA probe captures a CHA product triggered by a target gene, and MB and ICG are incubated respectively to serve as electrochemical and photo-thermal signal probes and used for detecting the SRB DsrC gene. Compared with a single-mode biosensor, the dual-mode biosensor has the advantages of rapidness, sensitivity, low background signal and strong anti-interference capability of electrochemical sensing and photo-thermal sensing, and the detection accuracy is improved through mutual verification of the two modes.

The invention discloses a Hannaea yeast ZAFU HH1, which is classified and named as Hannaea zeae, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on June 28, 2023, and the preservation number is CGMCC No.27725. The Hannaea yeast ZAFU HH1 is named as Hannaea zeae and is preserved in China General Microbiological Culture Collection Center (CGMCC) on June 28, 2023. The invention also discloses a microbial inoculum prepared from the strain. The invention also discloses application of the bacillus subtilis and a microbial agent prepared from the bacillus subtilis in conversion of zearalenone. The yeast Hannai ZAFU HH1 has excellent ZEN conversion capability, the conversion product is identified as a derivative with lower toxicity, and the safety of the ZEN conversion product is evaluated by using caenorhabditis elegans as a model organism, so that the conversion product is proved to have good biological safety. In addition, under the conditions of low temperature stress and acid stress, the yeast Hannai ZAFU HH1 still keeps a high ZEN conversion rate.

This invention belongs to the field of biosensors and provides a fePor-tpa-based colorimetric-photoelectrochemical dual-mode biosensor for the detection of Staphylococcus aureus. Bulk fePor-tpa films and two-dimensional fePor-tpa films were synthesized on ITO under solvothermal conditions and in situ growth. FePor-tpa originates from the catalytic activity of porphyrin iron and exhibits excellent dual-enzyme performance. By cleverly utilizing the peroxidase of the large-volume fePor-tpa film in colorimetric mode to oxidize TMB, and utilizing the catalase of the 2D fePor-tpa film in PEC mode to generate O2, the invention provides a solution for the detection of Staphylococcus aureus. 2 We constructed colorimetric and PEC dual-mode biosensors for the sensitive and specific quantification of Staphylococcus aureus.

The invention relates to the technical field of biology, and provides a cell culture meat food safety evaluation method based on a nematode model, and the nematode model is a caenorhabditis elegans model, comprises individuals at the L1 stage and the L4 stage development stage, and is used for establishing a chronic exposure model and an acute exposure model. Biological parameters are obtained by measuring the model of the cell culture meat protein after ingestion of the marker, and the parameters are compared and analyzed with parameters of a control group, so that the food safety of the cell culture meat protein is evaluated. According to the method, caenorhabditis elegans is taken as a model organism, the provided cell culture meat food safety evaluation method is simple and rapid, low in cost, short in test period and good in repeatability, early warning is provided for potential toxic components in food, and the safety evaluation process is accelerated.

The invention discloses selenium-enriched spirulina carbon dots as well as a preparation method and application thereof, and belongs to the technical field of medicines. The selenium-rich spirulina powder is obtained by taking selenium-rich spirulina growing to a logarithmic phase as a raw material, centrifuging, collecting and freeze-drying. Uniformly mixing the spirulina platensis with a catalyst and deionized water according to a mass ratio of 1: (0.1-0.8): 20, then putting the mixture into a high-temperature reaction kettle to react at 140-260 DEG C for 1.5-5.5 hours, dialyzing the reaction liquid for 24 hours after the reaction is finished, centrifuging, and freeze-drying the dialysate to obtain the selenium-enriched spirulina platensis carbon dots with the yield of 70-95%. The selenium-enriched spirulina carbon dots provided by the invention not only have the inhibition capability of alpha-amylase, alpha-glucosidase, cholesterol esterase and pancreatic lipase and the cholate binding capability, but also can improve the endogenous antioxidant defense capability of model organisms while removing exogenous oxidizing substances, so that the glycolipid metabolism balance is efficiently regulated and controlled.

The present invention provides high-content and high-resolution in vivo screening for the analysis of the function of multiple genes. In the functional screening of the present invention, genetic perturbations are delivered to CRISPR-expressing transgenic systems by specific AAV vectors fully compatible with the Perturb-seq platform. This screening enables in vivo functional genomics analysis in a variety of tissues, cell types, and model organisms with high-throughput single-cell readout.

This invention discloses a remotely controllable inertial-electrostatic field multi-mode bioaerosol sampling system. The system includes an inertial bioaerosol sampler, an electrostatic field bioaerosol sampler, and a remote controller. The inertial sampler captures aerosol particles using the principle of inertial impaction and is equipped with sampling heads with different flow rates of 1000 / 1300 / 1500 L / min. The electrostatic field sampler employs the principles of corona discharge and electrostatic adsorption, with a uniquely designed air duct that creates a descending and then ascending inlet airflow and a uniformly flowing outlet airflow. When used together, the two systems achieve a capture efficiency of ≥98% for particles larger than 1µm, with a wide particle size sampling range of 0.01-100 µm. The remote controller wirelessly controls the two samplers at a distance of ≥100 m, enabling synchronous or time-sharing multi-mode sampling of aerosols with a wide particle size range. The advantage of this invention is that it balances high efficiency and high flow rate sampling of aerosols with a wide particle size range while reducing biosafety risks to personnel.

This invention belongs to the field of environmental toxicology and molecular detection technology, and provides a rapid and accurate method for detecting ferroptosis induced by environmental pollutants and its applications. This method utilizes early zebrafish embryos as a model organism and combines morphological, biochemical, and molecular biological indicators to establish a standardized ferroptosis detection system for identifying and quantitatively evaluating pesticide exposure-induced ferroptosis. This method features a short detection cycle (completed within 96 hours), high sensitivity, and strong specificity, effectively distinguishing between general oxidative stress and iron-dependent cell death, providing a novel detection tool for pesticide environmental toxicology research and ecological risk assessment.

The present application belongs to the technical field of zooplankton culture, and particularly relates to a culture medium for metal chronic toxicity experiment and application thereof. The culture medium provided by the present application does not contain EDTA or other chelating agents, and can also meet the normal reproduction and growth requirements of model organisms, is suitable for long-term culture of model organisms, is conducive to reducing the influence of the components of the culture medium on the bioavailability and toxicity evaluation and determination results of metals, improving the accuracy and reliability of the evaluation results, and thus more accurately determining the chronic toxicity or bioavailability of rare earth elements, and is conducive to accurately exploring the long-term ecological risk of rare earth elements, and provides a new reference approach for metal environmental standard formulation and scientific environmental decision-making.

This invention discloses the application of glycyrrhizin in the preparation of products that extend lifespan. In a specific embodiment of this invention, *C. elegans*, a model organism for lifespan and aging, was used as the research subject. It was found that glycyrrhizin can extend the lifespan of *C. elegans*, demonstrating that glycyrrhizin has good anti-aging effects and can be applied to the preparation of products that delay aging or prevent or treat age-related diseases.

The invention discloses a construction method of an ischemic nerve injury animal model, and belongs to the technical field of animal disease models.The method comprises the steps that juvenile zebrafish serves as a research object, and after being treated with a cobalt chloride aqueous solution, the juvenile zebrafish is subjected to behavioral detection and brain Hamp; e, dyeing and red blood cell red fluorescence labeling detection to realize multi-dimensional evaluation; according to the method, the model organism of the juvenile zebrafish is applied, the limitation of a traditional mammal model is broken through, the experiment cost is greatly reduced, meanwhile, the reliability of the model is improved by adopting a behavior-tissue-blood flow multi-dimensional evaluation system, and high-throughput screening is adapted by relying on the characteristics of the juvenile zebrafish; and an efficient tool is expected to be provided for research and development and mechanism research of neuroprotective drugs.

The invention belongs to the field of probiotic functional food, and relates to lactobacillus gasseri BDUP and application thereof in improving or enhancing learning ability, multi-dimensional memory and / or attention. The Lactobacillus gasseri BDUP can promote nerve cells to secrete a plurality of neurotransmitters, can significantly improve short-term memory, long-term memory, spatial memory and other multi-dimensional memory-related behavior expressions in model organisms and normal adult mice, and also can up-regulate the expression levels of brain-derived neurotrophic factors and synaptic-related proteins in hippocampus tissues. After the BDUP is ingested, the learning efficiency, the attention level and the memory performance of student groups, adult mental workers and old people are remarkably improved. Through multi-level system verification of cells, model organisms, animals and people, the comprehensive enhancement effect of the lactobacillus gasseri BDUP on learning ability and multi-dimensional memory in a normal physiological state is comprehensively disclosed, and a new technical scheme is provided for development of cognitive support food.

This invention discloses an all-optical three-dimensional scanning confocal fluorescence microscopy imaging device and its implementation method. The invention employs a deep learning-driven adaptive control method, using CNN to process spatially distributed data, dynamically controlling the phase hologram, laser intensity, and phase compensation to ensure aberration-free axial displacement of the excitation light focus. It also optimizes the bidirectional parallel optical scanning trajectory of the two-dimensional scanning system to achieve all-optical three-dimensional scanning. Combining LSTM and TCN, it obtains time-dependent relationships and integrates control of the laser source, adjustable aperture, electrically adjustable focusing lens, and photodetector. This enables efficient synchronous and automated operation and high-speed axial focusing adjustment, eliminating errors and time-series mismatches, automatically adapting to different sample optical characteristics and environmental interference, maintaining high-quality imaging, and improving system robustness and applicability. This invention is used for real-time tracking of embryos in model organisms, dynamic visualization of intracellular signaling molecules, observation of cell membrane protein migration and aggregation, and tracking of intracellular organelle interactions.

In one aspect, the disclosure relates to a platform for producing autogenic engineered living materials, the platform including at least a plurality of engineered cells, wherein each engineered cell expresses at least one β-solenoid protein monomer. In an aspect, the β-solenoid protein monomer can be a CsgA analog from a non-model organism such as, for example, an extremophile. In a further aspect, the β-solenoid protein monomer has customizable properties and can further be engineered to perform additional functions such as, for example, binding to specific target molecules. Also disclosed herein are a method for producing β-solenoid protein monomers by culturing the disclosed platform, hydrogels incorporating the β-solenoid monomers, and methods for 3D printing using the disclosed hydrogels as inks.

This invention provides a method for rapid detection of the toxicity of a mixture of microplastics and antibiotics, and its application, belonging to the field of environmental ecotoxicology. This invention uses *Chlorella vulgaris* as a model organism, culturing *Chlorella vulgaris* in its logarithmic growth phase under low-dose stress with an optimized culture medium and a mixture of microplastics and antibiotics to obtain a *Chlorella vulgaris* stock solution that responds more rapidly and has a more stable signal to pollutants. Then, through grouped experiments, the Fv / Fm and OD values ​​of the control and experimental groups were measured. 681 The method described in this invention calculates the relative change rate of algae in response to complex pollutants, and determines toxicity or toxicity response thresholds based on the relative change rate. This significantly improves the sensitivity and accuracy of algal responses to complex pollutants and can be effectively used for aquatic environment monitoring / assessment and / or aquatic organism risk early warning.

The invention discloses an application of a spinosad derivative in preparation of drugs for resisting diabetes and related diseases thereof and / or improving insulin resistance. The spinosad derivative is a compound as shown in a structural general formula (I), a salt or an optical isomer of the compound, wherein R1 is selected from NR8R9, a nitrogen-containing heteroaromatic ring of C5-C8, a nitrogen-containing aliphatic ring of C3-C7, a substituted nitrogen-containing heteroaromatic ring of C5-C8 and a substituted nitrogen-containing aliphatic ring of C3-C7; substituent groups on a substituted C5-C8 nitrogen-containing aromatic heterocyclic ring and a substituted C3-C7 nitrogen-containing aliphatic ring are independently selected from C1-C8 alkyl groups; r < 8 > and R < 9 > are independently selected from hydrogen, C1-C8 alkyl, R < 10 > NR < 11 > R < 12 >; and R8 and R9 are not hydrogen at the same time. Experiments prove that the spinosad derivative can significantly reduce the blood sugar level of diabetic model organisms, improve insulin resistance and regulate related indexes of liver glycometabolism, and has an anti-diabetic effect.

The invention belongs to the technical field of biology, and provides a method for identifying a ribosome DNA autonomous replication sequence and application. A ribosome DNA sequence of a target bacterium is obtained by adopting a three-section amplification theory, free plasmids are constructed, the target bacterium is transformed, and the ribosome DNA autonomous replication sequence of the target bacterium is screened by determining the transformation efficiency and evaluating the existence form and loss rate of the plasmids. According to the invention, the DNA autonomous replication sequence is identified from ribosome DNA, which is easier than that from genomes, especially for non-model organisms. The technology belongs to an enabling technology, and solves the problems of high complexity and low success rate of the prior art. In addition, a three-section amplification strategy is adopted to identify the independent replication sequence of the ribosome DNA, so that the method is simpler, more convenient and quicker than the existing method, the identification of the independent replication sequence of multiple groups of species is facilitated, genetic tools are perfected, and the technical development and application development of microorganisms are assisted.

The invention belongs to the technical field of biology, and particularly relates to a siRNA screening system for intervening chlamys farreri chain metabolism and application of the siRNA screening system. The siRNA screening system is used for inhibiting expression of metabolism genes of chlamys farreri, the metabolism genes are CYP46A1, GSTM1 and ABCF2, the nucleotide sequences of the siRNA are shown as SEQ ID NO.1-11, and the siRNA screening system comprises five kinds of siRNA aiming at the CYP46A1, three kinds of siRNA aiming at the GSTM1 and three kinds of siRNA aiming at the ABCF2. The invention provides siRNA for intervening expression of Chlamys farreri metabolic genes (CYP46A1, GSTM1 and ABCF2), and key technical indexes of application of RNAi outside model organisms are perfected at the same time. The technical content of the invention is of great significance for identifying genes playing a key role in a metabolic process, researching and developing a prevention and control technology for harmful substances in scallops, further cultivating new varieties with stress resistance characteristics, and promoting healthy development of scallop culture industry.

The application provides a bitter gourd low-temperature tolerance gene McADF09 and application thereof, and belongs to the technical field of genetic engineering. The application studies expression rules in a cold-resistant material RM and a cold-sensitive material SM of the bitter gourd, and finds that the McADF09 gene plays an important role in plant resistance to low-temperature stress. The application overexpresses the gene in a model organism Arabidopsis thaliana, and the low-temperature stress resistance of the Arabidopsis thaliana plant is enhanced. Therefore, the McADF09 gene and the protein coded by the McADF09 gene can regulate the cold resistance of the plant, and provide a new molecular target for preparation of cold-resistant plants.

The invention discloses a plastic foam toxicity evaluation method combining light aging simulation and biological detection, and belongs to the technical field of environmental health and microplastic toxicology. The method comprises the following steps: firstly, carrying out accelerated light aging on plastic foam particles under a simulated sunlight condition, and characterizing physicochemical properties (including surface morphology, particle size distribution and functional group change) of the plastic foam particles; then, carrying out toxicity detection by utilizing a model organism caenorhabditis elegans, and systematically evaluating the biological effect of the light aging EPS through indexes such as egg laying amount, movement behavior and body length. According to the method, the light aging process of the plastic foam in the environment can be truly represented, the incidence relation between the physicochemical change and the toxicity effect can be established, and an effective experimental basis and methodological support are provided for scientific evaluation of the environmental health risk of the plastic foam and product safety supervision.

The utility model relates to the technical field of experimental auxiliary instruments, and discloses a handheld micro-mode organism large-scale synchronization collector which comprises a transparent shell (6), a disposable suction head (1), a disposable centrifugal tube (4) and a negative pressure pump (5), and the disposable suction head (1), the disposable centrifugal tube (4) and the negative pressure pump (5) are arranged in the transparent shell (6) and are sequentially connected. The disposable centrifugal tube (4) is connected with the negative pressure pump through a disposable hose (2), the bottom end of the disposable centrifugal tube (4) is exposed out of the transparent shell (6), the disposable centrifugal tube (4) is fixedly connected with the transparent shell (6) through a detachable tube cap (3), and the detachable tube cap (3) is in threaded connection with the disposable centrifugal tube (4). And electric collection can be carried out after the negative pressure pump (5) is connected through a pipeline, so that the working efficiency of operators is improved.