177 results about "Model organism" patented technology

A robotic architecture for capturing the autonomous performance advantages the animal models enjoy in the natural environment is disclosed. A biomimesis process is employed to allow selective utilization of basic physical components and adaptation of a common control paradigm for each of different vehicle types. The biomimetic architecture involves five functional elements: a basic biomorphic plant for capturing the biomechanical advantages of the model organism; a neural circuit-based controller consisting of a finite state machine; myomorphic actuators producing linear graded force in response to trains of current pulses for mediating movements; labeled line code output by neuromorphic sensors; and a reactive behavioral sequencer executing command sequences defined within a behavioral library.

This invention provides methods for detecting and predicting drug interaction. In one embodiment of the invention, a plurality of cellular constituents in a biological sample is monitored as the sample is subject to various drug treatments. The response of the cellular constituents are analyzed to detect interactions. This method is particularly useful for predicting drug interaction using a model organism. It is also useful for analyzing interaction between any perturbations to a biological system.

The invention discloses a method for establishing a fragile X-syndrome non-human primate model on the basis of a CRISPR gene knockout technology. The method comprises the following steps: (1) establishing a FMR1 gene knockout machin model; (2) carrying out identification and related functional analysis on the machin model; (3) carrying out tests on the nerve characteristics and learning and memorizing ability of the machin model. The method utilizes a CRISPR gene knockout technology to establish a fragile X-syndrome non-human primate model. The model fills the blank of non-human primate model, can effectively stimulate the pathological process of human diseases, can be used as an optimum animal model for researching human diseases, can effectively predict the effect of novel vaccine, novel drug or novel diagnostic reagent in clinical applications, and thus greatly reduces the risk of novel drug development.

The present invention provides a system and method for analysis of multimodal imaging and non-imaging biomedical data, using a multi-parametric data representation and integration framework. The present invention makes use of (1) dimensionality reduction to account for differing dimensionalities and scale in multimodal biomedical data, and (2) a supervised ensemble of embeddings to accurately capture maximum available class information from the data.

The invention relates to organisms and compositions comprising one or more stem cells or one or more embryos, wherein the one or more stem cells or one or more embryos comprise one or more of the following mutations: (i) a deletion mutation; (ii) a knockout mutation; and/or (iii) an addition of a heterologous nucleic acid sequence; wherein the one or more mutations of (i), (ii), and/or (iii) are site-specific mutations caused by a Xanthomonas TAL nuclease (XTN). The invention also relates to method of mutating an embryo, iPS cell, stem cell, or more particularly a spermatogonial stem cell by exposing the nucleic acid sequence contained within such embryos or cell with a Xanthomonas TAL nuclease.

A method is disclosed for rapid molecular profiling of tissue or other cellular specimens by placing a donor specimen in an assigned location in a recipient array, providing copies of the array, and performing a different biological analysis of each copy. The results of the different biological analyses are compared to determine if there are correlations between the results of the different biological analyses at each assigned location. In some embodiments, the specimens may be tissue specimens from different tumors, which are subjected to multiple parallel molecular (including genetic and immunological) analyses. The results of the parallel analyses are then used to detect common molecular characteristics of the genetic disorder type, which can subsequently be used in the diagnosis or treatment of the disease. The biological characteristics of the tissue can be correlated with clinical or other information, to detect characteristics associated with the tissue, such as susceptibility or resistance to particular types of drug treatment. Other examples of suitable tissues which can be placed in the matrix include tissue from transgenic or model organisms, or cellular suspensions (such as cytological preparations or specimens of liquid malignancies or cell lines).

The invention discloses a new method for screening an anti-liver injury medicament by using a model organism zebra fish. The method comprises the following steps: continuously molding the zebra fish serving as a molding object for 3 days by using preferred carbon tetrachloride at the concentration of 1mmol/L to obtain the zebra fish of a liver injury model, and then evaluating the anti-liver injury activity of different receptor medicaments by using ALT and AST levels in the liver tissue of the zebra fish and the pathological change condition of the liver tissue of the zebra fish. Experimental results show that the new method for screening the anti-liver injury medicament by using the model organism zebra fish has high molding success rate, can simulate an in vivo environment to perform quick screening, and has the advantages of strong operability, accurate experimental results, little amount of required reacceptance medicament, strong repeatability, low cost, wide application range, capability of performing batch screening experiments and high work efficiency.

A technique for simulating the activities of a large number of creatures is described. The technique utilises two modes of simulation. The first mode of simulation is arranged to simulate the activities of all of the creatures. The second mode of simulation is arranged to simulate the activities of a few of the creatures, at a more detailed level than the first mode. The second mode is utilised by the first mode to determine new parameters of individual creatures when the creatures undergo a change in environment.

The invention discloses a method for carrying out toxicity analysis on tail water of a sewage treatment plant by using caenorhabditis elegans. The method comprises the following steps of (1) cultivation of the caenorhabditis elegans; (2) cultivation of escherichia coli OP 50; (3) anabiosis of the caenorhabditis elegans; (4) passage of caenorhabditis elegans; (5) synchronization of the caenorhabditis elegans; and (6) testing the comprehensive toxicity of a to-be-detected tail water sample by using the caenorhabditis elegans. The method provided by the invention has the beneficial effects that model organism-caenorhabditis elegans is utilized to carry out toxicity analysis on the tail water of the sewage treatment plant, and the size of the comprehensive toxicity of the waste water is judged by contrasting the difference of fatality rate, reproduction rate, motor behavior and generation cycle of nematodes and exposing nematodes of a blank control group.

The invention discloses a single nucleotide polymorphism (SNP) site identification method based on digestion library-establishing and sequencing and bayesian statistics. The method is used for processing RAD (restriction site associated deoxyribonucleic acid) sequencing data, searching candidate SNP on an RAD sequencing fragment, and identifying the SNP reliability by employing a bioinformatics analysis method based on bayesian statistics. The method can be used for model and non-model organisms to eliminate the limitation that lots of species are lack of reference sequences and reduce the sequencing cost, and can be used for solving the bottleneck that a reliable statistical method is absent in the process of performing SNP identification by utilizing the RAD data at present, so that the obtained SNP site accuracy is greatly improved.

The invention relates to the technical field of information storage, in particular to a DNA-based information storage method. A long sequence in vivo DNA information storage technique is provide. Themain goal is to construct a coding system with strong error correction mechanism based on LDPC and BCH codes, which can reduce the redundancy of primers and indexes by long sequence coding, and achieve a high actual carrying capacity (above 97%). The main goal is to construct a medium-length DNA sequence (above 1Kbp). Saccharomyces cerevisiae in vivo assembly system is used to assemble and store long sequences and preserve information, and the information can be replicated at low cost, high fidelity and high speed by model organisms such as Saccharomyces cerevisiae, Escherichia coli or Bacillus. At the same time, because of the existence of strong error correction system, the data in the bacterial cell can be reduced perfectly with low coverage (1-5 X) in the second generation and third generation sequencing.

The invention discloses a komagataeibacter nataicola RZS01 strain which is preserved in China General Microbiological Culture Collection Center with a preservation numberof CGMCC No.10961. The bacterial strain is separated from defective fruits, and the separating and screening method is simple and convenient, so that the bacterial strain is easy to obtain. The bacterial strain is Gram-negative and short-bar-shaped aerobic bacteria, and is stable in performance. The komagataeibacter nataicola RZS01 is the bacterial cellulose producing bacteria, and can be used for producing bacterial cellulose through flask shaking fermentation, static state fermentation, fermentation tank large-scale fermentation or the combination of any two or three of the flask shaking fermentation, the static state fermentation and fermentation tank large-scale fermentation, the cellulose yield is high, large-scale industrial production is facilitated, and remarkable economic benefits are brought. The komagataeibacter nataicola RZS01 is nontoxic, free of pathogenicity, safe to people and livestock, and friendly to environment, serves as model organism for bacterial cellulose production, and has potential application prospects.

The invention relates to a construction method of a caenorhabditis elegans hyperuricemia model. The method comprises the following steps that 1, the uric acid content in caenorhabditis elegans is measured through a high performance liquid chromatography method; 2, an optimal drug delivery and culture mode is determined; 3, an optical drug delivery substance and drug delivery dosage are determined; 4, the optimal drug delivery time of xanthine is determined; 5, series experiment evaluation is conducted on the caenorhabditis elegans hyperuricemia model obtained through the construction method, and the caenorhabditis elegans hyperuricemia model which is low in damage, efficient, stable and reliable is obtained. According to the construction method, the hyperuricemia animal model is constructed by means of the model organism caenorhabditis elegans which has the high conservative property to human genes and has the similar uric acid metabolic pathway with human beings, the model has typical pathological characterization and is stable and reliable, various quantity indexes have the statistical significance, and a powerful tool is provided for large-scale and high-throughput drug screening of gout and the hyperuricemia symptom.

The invention relates to an organism nutrient solution, in particular to a freshwater rotifer common nutrient solution; the preparation method is that potassium nitrate, magnesium sulfate heptahydrate, calcium nitrate hexahydrate, dipotassium phosphate, vitamin B12 and vitamin H are added into phosphate buffer with pH of 7.0-7.5. The invention with different proportions is added to distilled water to prepare the rotifer nutrient solution which is applicable to the common types of freshwater rotifers, and the nutrient solution has high stability, simple preparation, no poison and no harm and wide applicable scope, provides conditions for the indoor culture of the model organism rotifer studied in experimental ecology, improves the indoor culture quality of the freshwater rotifer and reduces the risk of rotifer cultivation.

The purpose of the invention is to provide a method with easy operation and high accuracy for screening toxicity of traditional Chinese medicine by model organism zebra fish. The method comprises the following steps: dissolving a crushed or extracted traditional Chinese medicine material or a patented Chinese herbal prescription into water, preparing test solutions with different concentrations, selecting healthy and active zebra fish with similar sizes, and putting the zebra fish randomly into clean glass jars with an experimental temperature of (20+/-2) DEG C, continuously observing for 4 hours after the experiment begins, simultaneously carrying out untime observation within 120 hours at a frequency of at least one time observation in every 6 hours, picking out dead fish timely, when the fish fries are poisoned to stop breathing, clamping the fishtail petioles with a pincette, determining that the fish fry is dead when no response is observed in 10 min], calculating and recording accumulated mortality of the zebra fish of each group, and calculating LC 50 and 95% confidence interval by utilizing a karbers method. The method is characterized by a simple operation method and a strong practicality; the method can preliminarily determine the toxicity of the traditional Chinese medicine with a fast speed and a high accuracy.

The present invention discloses a novel method for model organism zebrafish evaluation of osteoporosis prevention treatment drug effects. The method includes selecting zebrafish as a model animal, grouping and designing experiments by an optimized experimental grouping method, administering by using a preferable drug administration method, using a preferable method to dye zebrafish bones with alizarin red, capturing images by intuitive microscopic observation, and analyzing bone dyed areas to reflect the amount of bone mineralization by using a preferable image analysis software. The entire experimental method is high in operability, can objectively reflect the effects of the drug in zebrafish in vivo on bone mass generation, and can quickly and accurately evaluate the effects of compounds and complex ingredients of traditional Chinese medicine in the prevention and treatment of osteoporosis. The method can overcome the shortcomings that ordinary in-vitro cell experiments are rigor in experimental conditions and difficult to reflect body effect comprehensive results; and overcomes shortcomings that mammal in-vivo experiments are time-consuming, large amount of drug are tested, and labor intensity is high. The method is high in accuracy of experimental results, the experimental method is high in repeatability, the required drug to be tested is relatively less, the cost is low, labor intensity is low, application range is wide, the experimental studies can be conducted in batch, and the working efficiency is high.

The invention, belonging to the technical field of medicament, particularly relates to a screening method of medicines for inducing the formation of myocardial cells by using a model organism zebrafish, comprising the following steps: first, letting 25-30 pairs of Tg (cmlc2: EGFP) transgenic zebrafish homozygotes mate to obtain embryons, then transferring the embryons into a culture plate, adding a proper amount of zebrafish embryons at the stage of 50% epiboly in every pore, using an embryon culture solution containing 1-30 mu M small molecular compounds in an incubator for culture; then carefully checking the embryon heart size, heart shape and the number of myocardial cells; in addition, checking the whole shape (such as dorsal-ventral body axis, anterior-posterior body axis, etc.) and whether the formation of other organs of the embryons is influenced; and finally preliminarily judging whether the small molecular compounds promote the formation of the myocardial cells without influencing the growth of the other organs according to the experiment results. The new medicines which are screened out by the method has strong specificity and low toxic and side effect.