9 results about "Model organism" patented technology

In certain aspects, the invention provides methods and compositions for heritable immunization and their use in infectious disease control. The invention also provides methods of preparing organisms and model organisms that can heritably transfer immunization to offspring.

The present application relates to the technical field of water quality restoration, and more particularly to a multi-source sensing intelligent closed-loop water quality restoration system and a control method thereof, which is suitable for river management, landscape water maintenance, industrial wastewater treatment and the like, and is particularly suitable for small and medium-sized water body restoration projects that need to dynamically respond to water quality changes. By means of multi-channel online monitoring, physicochemical index data and model organism activity data are obtained, combined with artificial sampling data, an evaluation model is used to comprehensively evaluate the water quality and grade the abnormality level; and according to the abnormality level, a graded restoration program is automatically triggered, and automatic backflow, automatic sample reservation and remote alarm are simultaneously executed, so as to realize closed-loop, accurate and efficient restoration of water quality and whole-process traceable management, improve restoration efficiency and reliability, and reduce the frequency of manual intervention.

The present invention provides high-content and high-resolution in vivo screening for the analysis of the function of multiple genes. In the functional screening of the present invention, genetic perturbations are delivered to CRISPR-expressing transgenic systems by specific AAV vectors fully compatible with the Perturb-seq platform. This screening enables in vivo functional genomics analysis in a variety of tissues, cell types, and model organisms with high-throughput single-cell readout.

This invention discloses a remotely controllable inertial-electrostatic field multi-mode bioaerosol sampling system. The system includes an inertial bioaerosol sampler, an electrostatic field bioaerosol sampler, and a remote controller. The inertial sampler captures aerosol particles using the principle of inertial impaction and is equipped with sampling heads with different flow rates of 1000 / 1300 / 1500 L / min. The electrostatic field sampler employs the principles of corona discharge and electrostatic adsorption, with a uniquely designed air duct that creates a descending and then ascending inlet airflow and a uniformly flowing outlet airflow. When used together, the two systems achieve a capture efficiency of ≥98% for particles larger than 1µm, with a wide particle size sampling range of 0.01-100 µm. The remote controller wirelessly controls the two samplers at a distance of ≥100 m, enabling synchronous or time-sharing multi-mode sampling of aerosols with a wide particle size range. The advantage of this invention is that it balances high efficiency and high flow rate sampling of aerosols with a wide particle size range while reducing biosafety risks to personnel.

This invention belongs to the field of environmental toxicology and molecular detection technology, and provides a rapid and accurate method for detecting ferroptosis induced by environmental pollutants and its applications. This method utilizes early zebrafish embryos as a model organism and combines morphological, biochemical, and molecular biological indicators to establish a standardized ferroptosis detection system for identifying and quantitatively evaluating pesticide exposure-induced ferroptosis. This method features a short detection cycle (completed within 96 hours), high sensitivity, and strong specificity, effectively distinguishing between general oxidative stress and iron-dependent cell death, providing a novel detection tool for pesticide environmental toxicology research and ecological risk assessment.

This invention discloses the application of glycyrrhizin in the preparation of products that extend lifespan. In a specific embodiment of this invention, *C. elegans*, a model organism for lifespan and aging, was used as the research subject. It was found that glycyrrhizin can extend the lifespan of *C. elegans*, demonstrating that glycyrrhizin has good anti-aging effects and can be applied to the preparation of products that delay aging or prevent or treat age-related diseases.

This invention provides a method for rapid detection of the toxicity of a mixture of microplastics and antibiotics, and its application, belonging to the field of environmental ecotoxicology. This invention uses *Chlorella vulgaris* as a model organism, culturing *Chlorella vulgaris* in its logarithmic growth phase under low-dose stress with an optimized culture medium and a mixture of microplastics and antibiotics to obtain a *Chlorella vulgaris* stock solution that responds more rapidly and has a more stable signal to pollutants. Then, through grouped experiments, the Fv / Fm and OD values ​​of the control and experimental groups were measured. 681 The method described in this invention calculates the relative change rate of algae in response to complex pollutants, and determines toxicity or toxicity response thresholds based on the relative change rate. This significantly improves the sensitivity and accuracy of algal responses to complex pollutants and can be effectively used for aquatic environment monitoring / assessment and / or aquatic organism risk early warning.

The invention belongs to the technical field of biology, and provides a method for identifying a ribosome DNA autonomous replication sequence and application. A ribosome DNA sequence of a target bacterium is obtained by adopting a three-section amplification theory, free plasmids are constructed, the target bacterium is transformed, and the ribosome DNA autonomous replication sequence of the target bacterium is screened by determining the transformation efficiency and evaluating the existence form and loss rate of the plasmids. According to the invention, the DNA autonomous replication sequence is identified from ribosome DNA, which is easier than that from genomes, especially for non-model organisms. The technology belongs to an enabling technology, and solves the problems of high complexity and low success rate of the prior art. In addition, a three-section amplification strategy is adopted to identify the independent replication sequence of the ribosome DNA, so that the method is simpler, more convenient and quicker than the existing method, the identification of the independent replication sequence of multiple groups of species is facilitated, genetic tools are perfected, and the technical development and application development of microorganisms are assisted.

The application provides a bitter gourd low-temperature tolerance gene McADF09 and application thereof, and belongs to the technical field of genetic engineering. The application studies expression rules in a cold-resistant material RM and a cold-sensitive material SM of the bitter gourd, and finds that the McADF09 gene plays an important role in plant resistance to low-temperature stress. The application overexpresses the gene in a model organism Arabidopsis thaliana, and the low-temperature stress resistance of the Arabidopsis thaliana plant is enhanced. Therefore, the McADF09 gene and the protein coded by the McADF09 gene can regulate the cold resistance of the plant, and provide a new molecular target for preparation of cold-resistant plants.