50 results about "Model organism" patented technology

The invention discloses an all-optical three-dimensional scanning confocal fluorescent microscopic imaging device and an implementation method thereof. According to the invention, through a deep learning driven adaptive regulation and control method, CNN is adopted to process spatial distribution data and dynamically regulate and control a phase hologram and the light intensity and phase compensation of a laser, so that the focal point of exciting light is subjected to aberration-free axial displacement, a bidirectional parallel optical scanning track of a two-dimensional scanning system is optimized, and all-optical three-dimensional scanning is realized; lSTM and TCN are combined to obtain a time sequence dependency relationship, a laser light source, an adjustable diaphragm, an electric focus-adjustable lens and a photoelectric detector are integrally controlled, efficient synchronization and automatic operation and high-speed axial focusing adjustment are realized, errors and time sequence mismatch are eliminated, optical characteristics of different samples and environmental interference are automatically adapted, and high-quality imaging is kept. The robustness and the applicable scene range of the system are improved; the method is used for model biological embryo real-time tracking, intracellular signal molecule dynamic visualization, cell membrane protein migration and aggregation observation and intracellular organelle interaction tracking.

The invention discloses construction and application of a confinement enhanced photoelectrochemistry-electrochemiluminescence dual-mode biosensor, and relates to the technical field of biosensing and analytical chemistry. The preparation method of the biosensor comprises the following steps: taking a CdS (at) SiO2 (at) NaYF4: Yb / Tm nano composite material as a substrate to obtain a confinement enhanced photoelectrochemistry (PEC) and electrochemiluminescence (ECL) sensing interface; in combination with a DNA wheel nanostructure triggered by target acetamiprid, signal amplification is realized; and synchronous dual-mode quenching detection of PEC and ECL signals is realized by virtue of peroxidase-like catalytic precipitation and wide-spectrum absorption characteristics of the PtPd-CoSnO3 nanocube. The biosensor prepared by the invention has the advantages of high sensitivity, strong reliability, good selectivity and the like, can be applied to accurate detection of acetamiprid in samples such as agricultural products and the like, and has important application value in the aspects of environmental monitoring and food safety control.

In certain aspects, the invention provides methods and compositions for heritable immunization and their use in infectious disease control. The invention also provides methods of preparing organisms and model organisms that can heritably transfer immunization to offspring.

Methods and systems for automating surgical procedures in model organisms. The system includes a user input panel, a trained computer vision system, a server, and articulated robotic arm. The system may further include an analytical scale, an anesthesia chamber, a tube labeling system, a tissue freezing system, and a biohazard waste disposal system. The computer vision system communicates with the robotic arm to coordinate tissue collections and common research procedures such as injections via recognition models related to detection, identification, localization, and classification. The central server provides rapid synchronization of data between the local machine and a cloud account system for real-time data analytics. The anesthesia chamber provides standardized administration of anesthesia, the sample labeling system provides 2D barcoded labels according to user input, and the tissue freezing and waste disposal systems enable storage of samples and removal of carcasses following tissue collection, fully automating the necropsy.

The invention discloses an ADO (adenosine dioxygenase) gene deleted zebrafish model as well as a construction method and application thereof. According to the invention, ADOa gene knockout is carried out on a model organism (zebrafish) to successfully construct an ADO gene deleted zebrafish model. Based on the model, the function of the ADO gene in fish taurine metabolism can be researched more deeply, so that a new scientific basis is provided for understanding the ADO function of zebra fish and even other fishes, and meanwhile, the model can be used for drug screening and provides a theoretical basis and technical support for breeding and genetic improvement of aquaculture industry.

The application relates to the field of biotechnology, in particular to the application of Caenorhabditis elegans in constructing an animal model for detecting combined toxicity of antibiotics and metal ions. The application provides the application of Caenorhabditis elegans in constructing an animal model for detecting combined toxicity of antibiotics and metal ions. The application takes Caenorhabditis elegans as a model organism, and provides a simple, fast, low-cost, short-cycle and reproducible method for constructing an animal model for detecting combined toxicity. The application provides technical support for combined toxicity of antibiotics and metal ions in a natural environment and specific action aspects, and has practical application value.

The invention relates to a kanamycin fluorescence-electrochemical dual-mode biosensor based on a three-dimensional DNAzyme walker and application of the kanamycin fluorescence-electrochemical dual-mode biosensor, and belongs to the technical field of biosensing. The biosensor comprises a gold electrode AuE modified with a capture chain CP, a three-dimensional DNAzyme walker and a target recognition probe Apt / R of kanamycin, the three-dimensional DNAzyme walker comprises a G-quadruplex substrate chain embedded with MB, a DNAzyme chain with a self-protected annular structural domain and gold nanoparticles (AuNPs); the target recognition probe Apt / R is obtained by hybridizing an aptamer chain Apt and an aptamer complementary chain R. By adopting a fluorescence-electrochemical dual-mode detection method, the problem that single signal output is easily interfered by a false positive signal and a background signal is effectively solved, and the accuracy of kanamycin detection is remarkably improved.

The invention discloses a CRISPR / Cas12a responsive AIE MOF nano-enzyme hydrogel dual-mode biosensor as well as a construction method and application thereof, and belongs to the technical field of food safety rapid detection. In order to solve the technical problems that an existing salmonella detection method is tedious in operation, low in detection speed, dependent on special equipment and professional operators, prone to being polluted by aerosol, high in price and the like, a difunctional AIE MOF nano material with AIE fluorescence performance and POD activity is packaged into DNA hydrogel and is further combined with a CRISPR / Cas12a system, and the detection sensitivity of salmonella is improved. A biosensor for detecting salmonella is constructed, and a high-sensitivity, accurate and rapid method is provided for detecting salmonella in food safety.

The invention belongs to the crossing field of marine biotechnology and bioinformatics, and particularly relates to a method for high-throughput identification of natural functional oligopeptides. At present, bottlenecks still exist in development and application of various functional oligopeptides such as penetrating peptides and antibacterial peptides in non-model species, and the high adaptability of the existing functional oligopeptides in complex and diverse non-model organisms is limited mainly due to differences (amino acid preference and receptor specificity) among species. Therefore, the invention provides a method for high-throughput identification of functional oligopeptides from natural sequences of organisms by means of machine learning. According to the method, based on a biological natural protein sequence, an oligopeptide sequence set meeting a preset length condition is generated through a sliding window strategy system, a deep machine learning model is utilized to perform high-throughput functional prediction on the oligopeptide sequence, and on the basis of performing'interruption-prediction-re-comparison 'on existing functional oligopeptides, the functional oligopeptide sequence set meeting the preset length condition is obtained. The comparison threshold value for accurately identifying the functional oligopeptide can be determined, and the natural functional oligopeptide existing in the protein sequence can be effectively identified based on the threshold value. The functional oligopeptide obtained by the method has strict screening of physicochemical properties of a machine learning model and high-adaptability biological characteristics formed in species evolution. According to the method, the direct application capability of the functional oligopeptide in non-model species is remarkably improved, and an accurate and high-throughput technical means is provided for efficient development and application of the functional oligopeptide in non-model organisms.

The invention discloses a data processing device and method for predicting essential genes of non-model organisms in the field of bioinformatics and application. The technical problem to be solved by the invention is how to screen essential genes of non-model organisms. The method comprises the following steps: respectively obtaining a label corresponding to each protein from a protein sequence of a target species and protein sequences of n model biological species on the basis of annotations; embedding the protein sequence and the tag into the protein language model, and outputting an embedded vector; constructing a residue contact graph for each protein based on the embedded vector; modeling, integrating and outputting the features of each amino acid residue in the residue contact graph by using a graph neural network to obtain an integral protein embedding vector; inputting all the protein embedded vectors into a classifier model for training, and outputting a necessary probability value of a protein gene by using an activation function; and obtaining a necessary probability value P1 of the gene of the protein based on the classifier model. The invention can be applied to the prevention and control of agricultural diseases and the development of biopesticides.

The invention discloses a construction method and application of a zebra fish model. The construction method comprises the following steps: taking zebra fish as a model animal, and obtaining a zebra fish model by knocking out a sod1 gene in the zebra fish and / or expressing a human source sod1 gene mutant in the zebra fish; wherein the serial number of the sod1 gene in the zebra fish in an Ensepbl database is ENSDARG00000043848, and the nucleotide sequence of the human source sod1 gene mutant is as shown in SEQ ID NO. 1. The zebrafish can be used for the function research of the sod1 gene in the zebrafish, the function research of the human-derived sod1 gene mutant, the pathological research of amyotrophic lateral sclerosis and the screening of amyotrophic lateral sclerosis resisting drugs.

The invention belongs to the technical field of medicines, and particularly relates to application of vitamin B12 in preparation of a medicine for treating retinitis pigmentosa. Specifically, based on the high conservative property of human PRPF31 and caenorhabditis elegans prp-31 genes, a prp-31 gene knock-down and mutation nematode disease model is constructed, and the model can reproduce multiple pathological phenotypes such as oxidative stress, lysosome dysfunction and apoptosis caused by PRPF31 defects. Experiments prove that the active form (such as mecobalamin) of the vitamin B12 can actively pass through the blood retina barrier by virtue of an endogenous transport mechanism through oral administration, the pathological damage can be reversed by virtue of multi-channel synergy, and the pathological phenotype of a model organism can be remarkably improved, so that the vitamin B12 has a good practical application value.

The present application relates to the technical field of water quality restoration, and more particularly to a multi-source sensing intelligent closed-loop water quality restoration system and a control method thereof, which is suitable for river management, landscape water maintenance, industrial wastewater treatment and the like, and is particularly suitable for small and medium-sized water body restoration projects that need to dynamically respond to water quality changes. By means of multi-channel online monitoring, physicochemical index data and model organism activity data are obtained, combined with artificial sampling data, an evaluation model is used to comprehensively evaluate the water quality and grade the abnormality level; and according to the abnormality level, a graded restoration program is automatically triggered, and automatic backflow, automatic sample reservation and remote alarm are simultaneously executed, so as to realize closed-loop, accurate and efficient restoration of water quality and whole-process traceable management, improve restoration efficiency and reliability, and reduce the frequency of manual intervention.

The invention belongs to the technical field of bioengineering and analytical chemistry, and provides a probe eZinCh-2 modified plasmid based on FRET (Fluorescence Resonance Energy Transfer) and application of the probe eZinCh-2 modified plasmid in zinc ion detection. According to the invention, the plasmid is constructed through molecular cloning and homologous recombination technologies, and is microinjected into a zebrafish embryo, so that a transgenic strain capable of stably inheriting and expressing the probe protein is successfully obtained; as a living body biological tool, the zebrafish of the strain is combined with a confocal microscope spectrum FRET technology, and real-time and quantitative monitoring and visual imaging of dynamic changes of zinc ions at living body, cell and tissue levels are achieved. The method solves the problem that real-time dynamic visual monitoring of the zinc ions cannot be realized in a living body model in the prior art, and is suitable for real-time microscopic quantitative analysis of the content of the zinc ions in model organisms such as zebra fish and the like.

The invention relates to the modelling of a disorder called tubulinopathy, which refers to a wide range of cortical developmental malformations (CDM) resulting from defects in genes encoding tubulin proteins that regulate neuronal migration during brain development, by knocking out the tubulin gamma 1 (Tubg1) gene in zebrafish (Danio rerio) embryos using CRISPR / Cas9 technology. The transgenic animal in question allows the investigation of the phenotype and molecular pathological mechanisms of CDMs resulting from the deficiency of the Tubg1 protein during the developmental process. In the invention, the region located in the third exon of the Tubg1 gene in zebrafish is targeted and knocked out with a guide RNA having the nucleotide sequence SEQ ID NO: 1.

The invention belongs to the field of biosensor preparation methods, and particularly relates to a PNA-based electrochemical-photo-thermal dual-mode biosensor and a method for detecting an SRB DsrC gene. An electrode structure of the sensor is CHA / PNA / ITO, a PNA probe captures a CHA product triggered by a target gene, and MB and ICG are incubated respectively to serve as electrochemical and photo-thermal signal probes and used for detecting the SRB DsrC gene. Compared with a single-mode biosensor, the dual-mode biosensor has the advantages of rapidness, sensitivity, low background signal and strong anti-interference capability of electrochemical sensing and photo-thermal sensing, and the detection accuracy is improved through mutual verification of the two modes.

The invention discloses a Hannaea yeast ZAFU HH1, which is classified and named as Hannaea zeae, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on June 28, 2023, and the preservation number is CGMCC No.27725. The Hannaea yeast ZAFU HH1 is named as Hannaea zeae and is preserved in China General Microbiological Culture Collection Center (CGMCC) on June 28, 2023. The invention also discloses a microbial inoculum prepared from the strain. The invention also discloses application of the bacillus subtilis and a microbial agent prepared from the bacillus subtilis in conversion of zearalenone. The yeast Hannai ZAFU HH1 has excellent ZEN conversion capability, the conversion product is identified as a derivative with lower toxicity, and the safety of the ZEN conversion product is evaluated by using caenorhabditis elegans as a model organism, so that the conversion product is proved to have good biological safety. In addition, under the conditions of low temperature stress and acid stress, the yeast Hannai ZAFU HH1 still keeps a high ZEN conversion rate.

The invention discloses a method for screening diabetes treatment targets by using drosophila melanogaster as a model organism, and belongs to the technical field of biological medicines. According to the invention, a GAL4 / UAS system is utilized to construct transgenic drosophila melanogaster of which the specificity of a target gene (X) is knocked down, and F1-generation male ppl-GAL4 / + is obtained through hybridization; uAS-X RNAi / + drosophila melanogaster; compared with a high-glucose model control group with the same background, if the X gene knocks down, lipid droplet accumulation is reduced, and circulating glucose and trehalose are reduced (both have statistical significance, Plt, 0.05), the X gene is identified as a potential diabetes (abnormal glucose and lipid metabolism) treatment target. The method provided by the invention overcomes the problems of high cost, low efficiency, insufficient individuation and the like in the existing diabetes drug screening method, and provides an accurate, rapid, efficient and low-cost screening means for research and development of diabetes treatment drugs.

This invention belongs to the field of biosensors and provides a fePor-tpa-based colorimetric-photoelectrochemical dual-mode biosensor for the detection of Staphylococcus aureus. Bulk fePor-tpa films and two-dimensional fePor-tpa films were synthesized on ITO under solvothermal conditions and in situ growth. FePor-tpa originates from the catalytic activity of porphyrin iron and exhibits excellent dual-enzyme performance. By cleverly utilizing the peroxidase of the large-volume fePor-tpa film in colorimetric mode to oxidize TMB, and utilizing the catalase of the 2D fePor-tpa film in PEC mode to generate O2, the invention provides a solution for the detection of Staphylococcus aureus. 2 We constructed colorimetric and PEC dual-mode biosensors for the sensitive and specific quantification of Staphylococcus aureus.

Escherichia coli is a widely studied model organism and an integral component of the human gut microbiome, offering significant potential for bacteria-based therapeutic applications. However, engineering native E. coli strains poses persistent challenges. In this study, the chassis- independent recombinase-assisted genome engineering technique was leveraged to engineer the native gut strain E. coli EcAZ-1 and the probiotic strain E. coli Nissle 1917 (EcN). The bioluminescent lux operon, green fluorescent protein (GFP), and the oxygen-independent fluorescent protein IFP 2.0 were successfully introduced into both strains. To further enhance IFP 2.0 fluorescence, a heme oxygenase was co-expressed, and the chromophore biliverdin was supplemented, achieving robust IFP 2.0 expression under both anaerobic and aerobic conditions. Also, both strains were engineered to biosynthesize bioactive compounds, including the plant- derived flavonoid naringenin and mycosporine-like amino acids. The results underscore the potential of native E. coli strains as flexible and robust platforms for synthetic biology, enabling novel applications in biomedical research and therapeutics. Based on these results, this application provides recombinant bacteria generating IFP2.0 fluorescence under anaerobic condition, bacterial composition for gastrointestinal cancer detection and use method thereof, and naringenin or MAA overproducing recombinant bacteria.

The invention relates to the technical field of biology, and provides a cell culture meat food safety evaluation method based on a nematode model, and the nematode model is a caenorhabditis elegans model, comprises individuals at the L1 stage and the L4 stage development stage, and is used for establishing a chronic exposure model and an acute exposure model. Biological parameters are obtained by measuring the model of the cell culture meat protein after ingestion of the marker, and the parameters are compared and analyzed with parameters of a control group, so that the food safety of the cell culture meat protein is evaluated. According to the method, caenorhabditis elegans is taken as a model organism, the provided cell culture meat food safety evaluation method is simple and rapid, low in cost, short in test period and good in repeatability, early warning is provided for potential toxic components in food, and the safety evaluation process is accelerated.

The invention discloses selenium-enriched spirulina carbon dots as well as a preparation method and application thereof, and belongs to the technical field of medicines. The selenium-rich spirulina powder is obtained by taking selenium-rich spirulina growing to a logarithmic phase as a raw material, centrifuging, collecting and freeze-drying. Uniformly mixing the spirulina platensis with a catalyst and deionized water according to a mass ratio of 1: (0.1-0.8): 20, then putting the mixture into a high-temperature reaction kettle to react at 140-260 DEG C for 1.5-5.5 hours, dialyzing the reaction liquid for 24 hours after the reaction is finished, centrifuging, and freeze-drying the dialysate to obtain the selenium-enriched spirulina platensis carbon dots with the yield of 70-95%. The selenium-enriched spirulina carbon dots provided by the invention not only have the inhibition capability of alpha-amylase, alpha-glucosidase, cholesterol esterase and pancreatic lipase and the cholate binding capability, but also can improve the endogenous antioxidant defense capability of model organisms while removing exogenous oxidizing substances, so that the glycolipid metabolism balance is efficiently regulated and controlled.

The present application belongs to the technical field of biological toxicity test of chemicals, and particularly relates to a chemical toxicity test method based on a self-developed pollutant exposure system. The test method is used for simulating the toxicity of chemicals in natural water bodies by means of a self-developed pollutant exposure system, adopts zebrafish as a model organism, establishes a time-concentration linear function of the tested chemicals in a poisoning area, and calculates the toxicity test method of the minimum effect concentration of the tested chemicals by observing the abnormal behavior of zebrafish or analyzing the behavior by coupling a camera tracking technology. The test method is almost a non-destructive experiment, the experimental time is short, most of the zebrafish participating in the experiment can be restored to normal state through appropriate recovery, the time cost is greatly saved, and the test is efficient and has a low mortality rate of test organisms.

The application discloses a new use of rape pollen alkali A in nerve protection and brain disease prevention and treatment, discloses beneficial effects and molecular mechanisms of the rape pollen alkali A after the rape pollen alkali A acts on Caenorhabditis elegans, and provides significant differences of three groups (young group, natural aging group and rape pollen alkali A group) of the Caenorhabditis elegans on a transcriptome and a proteome, and reveals potential effects of the rape pollen alkali A on neurodegenerative changes and brain diseases caused by inflammatory substances such as leukotriene B4. The application finds that the rape pollen alkali A improves the pharyngeal pump and body swing of the Caenorhabditis elegans which gradually deteriorate with growth, and significantly improves the oxidative stress capacity of the Caenorhabditis elegans, and preliminarily verifies the effects of the rape pollen alkali A on neurodegenerative changes and brain diseases caused by inflammatory substances such as leukotriene B4 on a model organism. The effects of the rape pollen alkali A in the application are clear, and the rape pollen alkali A has application prospects in nerve protection and brain disease prevention and treatment caused by leukotriene B4.

Omeprazole is used as a widely applied proton pump inhibitor and is mainly used for treating various diseases caused by hyperacidity, such as gastric ulcer, duodenal ulcer, reflux esophagitis and the like. The invention relates to a novel application of a commercially available medicine omeprazole, namely the omeprazole has remarkable anti-aging activity. The research on model organism caenorhabditis elegans finds that omeprazole can significantly prolong the life of nematodes. Specifically, when the nematodes are cultured in culture media containing 1.6 mg / mL omeprazole and 3.2 mg / mL omeprazole, the service lives of the nematodes are prolonged by 47.80% and 61.51% respectively, and the effect is extremely rare in current drug research.

The present invention provides high-content and high-resolution in vivo screening for the analysis of the function of multiple genes. In the functional screening of the present invention, genetic perturbations are delivered to CRISPR-expressing transgenic systems by specific AAV vectors fully compatible with the Perturb-seq platform. This screening enables in vivo functional genomics analysis in a variety of tissues, cell types, and model organisms with high-throughput single-cell readout.

Omeprazole, as a widely used proton pump inhibitor, is mainly used for the treatment of various diseases caused by excessive gastric acid, such as gastric ulcer, duodenal ulcer and reflux esophagitis. The present application relates to a new use of a commercially available drug omeprazole, i.e. it has significant anti-aging activity. The present application found that omeprazole can significantly prolong the life of nematodes through research using model organisms Caenorhabditis elegans. Specifically, when the nematodes are cultured in a medium containing 1.6 mg / mL and 3.2 mg / mL omeprazole, their life is prolonged by 47.80% and 61.51% respectively, which is extremely rare in current drug research.

The application belongs to the technical field of biological detection, and particularly relates to a multi-mode biosensor and a preparation method and application thereof. The multi-mode biosensor takes a magnetic covalent organic framework as a substrate, takes a gold nanoparticle modified enrofloxacin aptamer as a recognition site, and is connected through adsorption of the substrate and the recognition site to construct the multi-mode biosensor. The multi-mode biosensor provided by the application is adsorbed on Fe3O4@COF through the Au NCs modified enrofloxacin aptamer through electrostatic adsorption and pi-pi stacking adsorption. Because the COF has excellent fluorescence quenching capacity, the red light of the Au NCs disappears, and the peroxidase-like activity is reduced. When the measured substance ENR is added, the aptamer specifically recognizes. Because the aptamer and the ENR have higher affinity, the aptamer falls off from the surface of the COF. The adsorption and falling off of the aptamer cause changes in the fluorescence signal and the enzyme activity, so that accurate and specific detection is performed.

This invention discloses a remotely controllable inertial-electrostatic field multi-mode bioaerosol sampling system. The system includes an inertial bioaerosol sampler, an electrostatic field bioaerosol sampler, and a remote controller. The inertial sampler captures aerosol particles using the principle of inertial impaction and is equipped with sampling heads with different flow rates of 1000 / 1300 / 1500 L / min. The electrostatic field sampler employs the principles of corona discharge and electrostatic adsorption, with a uniquely designed air duct that creates a descending and then ascending inlet airflow and a uniformly flowing outlet airflow. When used together, the two systems achieve a capture efficiency of ≥98% for particles larger than 1µm, with a wide particle size sampling range of 0.01-100 µm. The remote controller wirelessly controls the two samplers at a distance of ≥100 m, enabling synchronous or time-sharing multi-mode sampling of aerosols with a wide particle size range. The advantage of this invention is that it balances high efficiency and high flow rate sampling of aerosols with a wide particle size range while reducing biosafety risks to personnel.

This invention belongs to the field of environmental toxicology and molecular detection technology, and provides a rapid and accurate method for detecting ferroptosis induced by environmental pollutants and its applications. This method utilizes early zebrafish embryos as a model organism and combines morphological, biochemical, and molecular biological indicators to establish a standardized ferroptosis detection system for identifying and quantitatively evaluating pesticide exposure-induced ferroptosis. This method features a short detection cycle (completed within 96 hours), high sensitivity, and strong specificity, effectively distinguishing between general oxidative stress and iron-dependent cell death, providing a novel detection tool for pesticide environmental toxicology research and ecological risk assessment.