178 results about "Model organism" patented technology

A method is disclosed for rapid molecular profiling of tissue or other cellular specimens by placing a donor specimen in an assigned location in a recipient array, providing copies of the array, and performing a different biological analysis of each copy. The results of the different biological analyses are compared to determine if there are correlations between the results of the different biological analyses at each assigned location. In some embodiments, the specimens may be tissue specimens from different tumors, which are subjected to multiple parallel molecular (including genetic and immunological) analyses. The results of the parallel analyses are then used to detect common molecular characteristics of the genetic disorder type, which can subsequently be used in the diagnosis or treatment of the disease. The biological characteristics of the tissue can be correlated with clinical or other information, to detect characteristics associated with the tissue, such as susceptibility or resistance to particular types of drug treatment. Other examples of suitable tissues which can be placed in the matrix include tissue from transgenic or model organisms, or cellular suspensions (such as cytological preparations or specimens of liquid malignancies or cell lines).

A method for studying toxicity of drugs by using model organism zebra fish comprises the following steps of: selecting 100 to 200 adult zebra fishes, respectively disposing in bottles containing a solution of 30 to 50 mL, keeping the temperature in the bottles substantially constant at 22 to 25 DEG C, randomly grouping, each group containing 10 to 20 fishes, wherein the solution in one of the groups is pure water (blank), the solutions in other groups are medicinal solutions with different concentrations, and 0.5% to 2% dimethylsulfoxide (DMSO) can be added to assist dissolving a drug that has poor water solubility, when an additional solvent comparison group prepared by adding 0.5% to 2% DMSO into the pure water is needed; recording the death number of zebra fishes in the medicinal solutions of different concentrations within 24 h, and calculating the death rate (%); calculating median lethal dose-LD50 by Bliss method according to the experiment result; and representing the acute toxicity by the value of LD50. According to the method, the LD50s of zebra fish of triptolide, matrine and emodin are respectively 5.39*10<-3>, 112.3 and 1.08*10<3> mug / mL. The method can objectively reflect the toxicity of drugs.

The invention discloses an anti-aging evaluation method based on a model organism caenorhabditis elegans and an application of the method and relates to performance evaluation methods for phytochemicals, anti-aging medicines, healthcare products and the like. In the invention, under non-toxic concentration, the anti-aging effect of the to-be-tested substances is evaluated. With the evaluation method, existence of the anti-aging effect of the to-be-tested substances can be evaluated in a short period, wherein the evaluation is completed mainly through detection on life, heat stress capability,anti-ultraviolet irradiation capability, anti-oxidizing damage capability, reproductive capability, body length and lipofuscin content of the caenorhabditis elegans. The method is advantaged in that the model organism, caenorhabditis elegans, is short in growth period, so that the anti-aging effect can be observed rapidly in several weeks. The method has short experimental period and is convenientto carry out.

The invention discloses a bombyx mori glutathione-S-transferase BmGSTe4 gene with a nucleotide sequence shown in SEQIDNo.1, and the expression product thereof has glutathione-S-transferase (GSTs) activity, because GSTs plays an important role in the formation of pesticide resistance and oxidation resistance of insects, and bombyx mori is a lepidopterous insect-model organism, a biological control pesticide which can specifically kill lepidoptera pests and is harmless to other beneficial insects is expected to be designed through taking BmGSTe4 genes as targets. In addition, in the invention, a situation that BmGSTe4 genes have a function of inhibiting cell apoptosis is found, therefore, the BmGSTe4 genes can be used as potential targets of anti-cancer drug screening.

The invention provides a beta-amyloid plaque recognition and measurement method based on image processing, and belongs to the technical field of biological image processing. The method comprises the following concrete steps that a model organism brain slice is subjected to immunostaining by a beta-amyloid protein monoclonal antibody; after the immune dying, the extracellular beta-amyloid plaque ofthe model organism brain slice shows bright plaque in a fluorescence microscope; a fluorescence microscope is used for shooting a picture of the fluorescence microscope subjected to immunostaining; the picture is processed to recognize the located bright plaque region of the beta-amyloid plaque; through the image processing, the beta-amyloid plaque indicated by the bright plaque is subjected to automatic quantity statistics and feature calculation to obtain the statistics data; the statistics data is matched with feature information of the model organism for statistics analysis. The deposition condition of the beta-amyloid plaque is quantificationally reflected by a method of recognizing and measuring the bright plaque in the pictures shot by the fluorescence microscope.

The invention discloses a method for analyzing a structure of a transcriptome gene sequence of a non-model organism. The method comprises the following steps: (1) obtaining an optimal comparison result; (2) determining a protein coding mode and determining a translation termination position; (3) determining a coding starting position of the gene sequence; (4) classifying by utilizing a gene mode; (5) determining a nucleic acid sequence of a coding manner by utilizing a transcriptome sequence and training a nucleic acid sequence mode of a coding protein by utilizing a Markov chain; (6) determining a coding manner of a protein coding sequence of a gene which is not compared. The method disclosed by the invention is used for carrying out high-throughput structural analysis on a lot of gene sequences obtained by transcriptome sequencing of any non-model organism and function annotation of the transcriptome sequence is automatically finished in an analysis process; a Markov model and a support vector machine model are constructed by utilizing a compared high-reliability protein coding nucleic acid sequence, and a gene sequence which is not compared is analyzed, so that the credibility of the structural analysis of the sequence is higher.

The present invention provides a method comprising the use of microorganisms for nanotoxicity study and bioremediation. In some embodiment, the microorganisms are bacterial organisms such as Gram negative bacteria, which are used as model organisms to study the nanotoxicity of the fullerene compounds: E. coli W3110, a human related enterobacterium and Shewanella oneidensis MR-1, an environmentally important bacterium with versatile metabolism.

The invention discloses a method for knocking out a non-model organism plutella xylostella APN1 gene with CRISPR / Cas9 and an application of the method in molecular research on Bt Cry1Ac insecticidal protein resistance mechanisms. According to the method, a specific sgRNA target sequence of the plutella xylostella APN1 gene is designed and synthesized, sgRNA and Cas9 protein are mixed, the mixtureis microscopically injected into the posterior pole part of early embryonic disc stage eggs of plutella xylostella, plutella xylostella APN1 gene homozygous mutant populations with stable heredity arescreened out by a CRISPR / Cas9 gene editing system and applied to toxicity bioassay of Bt Cry1Ac insecticidal protein, which proves that plutella xylostella APN1 gene mutation can cause high resistance to Bt Cry1Ac insecticidal protein. The method is simple and efficient, time and labor are saved, technical support is provided for deep research on functional genes in non-model organisms, and a foundation is laid for exploring new pest control strategies.

A robotic architecture for capturing the autonomous performance advantages the animal models enjoy in the natural environment is disclosed. A biomimesis process is employed to allow selective utilization of basic physical components and adaptation of a common control paradigm for each of different vehicle types. The biomimetic architecture involves five functional elements: a basic biomorphic plant for capturing the biomechanical advantages of the model organism; a neural circuit-based controller consisting of a finite state machine; myomorphic actuators producing linear graded force in response to trains of current pulses for mediating movements; labeled line code output by neuromorphic sensors; and a reactive behavioral sequencer executing command sequences defined within a behavioral library.