309 results about "Protein coding" patented technology

The invention discloses an application of an OsSPL14 gene and a protein coded by the OsSPL14 gene in regulating and controlling the diameter of a plant root system, and belongs to the technical field of gene engineering. The technical problem to be solved by the invention is how to regulate and control the root diameter of a plant. In order to solve the technical problem, the invention provides application of the protein or a substance for regulating and controlling the expression of a protein coding gene or a substance for regulating and controlling the activity or content of the protein in regulating and controlling the diameter of a plant root system. The protein has an amino acid sequence of sequence 2. The invention discloses that OsSPL14 is an important gene for regulating and controlling the diameter of the rice root for the first time. The expression of the OsSPL14 is driven by using a specific promoter, so that the regulation effect of the OsSPL14 in a rice root system can be specifically exerted, and the diameter of the adventitious root of the rice is increased. The OsSPL14 provided by the invention has important significance in cultivating a new rice variety with high and stable yield, high quality and good stress resistance.

The invention discloses a cherry sugar transporter gene CpSWEET10 and application thereof, and belongs to the technical field of cherry gene engineering. A novel sugar transporter gene CpSWEET10 is obtained through cloning, the nucleotide sequence of the novel sugar transporter gene CpSWEET10 is shown as SEQ ID NO.1, and the coded amino acid sequence of the novel sugar transporter gene CpSWEET10 is shown as SEQ ID NO.2. The gene is highly expressed in cherry fruits, and the content of soluble solids and total sugar in mature fruits is remarkably positively correlated with the expression quantity of the soluble solids and the total sugar; through subcellular localization and sugar transport function defect type yeast mutant functional complementation experimental analysis, the protein coded by the CpSWEET10 gene is localized in a cytoplasmic membrane and has a sucrose transport function; the cherry sugar transporter gene CpSWEET10 disclosed by the invention can be applied to the improvement of the sugar degree character of the fruits, and a new gene resource is provided for molecular breeding for improving the high-sugar quality of the cherry fruits.

The invention discloses a sugarcane ScPP2C49 gene and application thereof, and relates to the technical field of plant biology, the nucleotide sequence of the ScPP2C49 gene is as shown in SEQ ID No.1, and the amino acid sequence of protein coded by the ScPP2C49 gene is as shown in SEQ ID No.2. The gene is induced to express by drought and abscisic acid, and the encoded protein of the gene is positioned in a cell nucleus. Functional studies show that after overexpression of the ScPP2C49 gene in the plant, the drought resistance of the plant is negatively regulated through various mechanisms of inhibiting an abscisic acid signal channel, hindering stomatal closure under drought stress, weakening active oxygen scavenging ability, reducing photosynthetic efficiency, inhibiting root growth and the like. The invention also provides a recombinant overexpression vector containing the gene and a method for obtaining a transgenic plant with reduced drought resistance by using the vector through an agrobacterium-mediated method. According to the invention, a new gene resource is provided for deep analysis of a plant drought-resistant molecular mechanism, and an important target gene is provided for cultivation of high-stress-resistance crop varieties through a reverse genetics means (such as gene knockout).

The invention provides an enzyme mining method, system and medium. The enzyme mining method comprises the following steps: inputting to-be-queried data; inputting the data to be queried into a pre-trained VenusRXN model; when the input to-be-queried data is a reaction, determining a reaction CLS embedding vector by using a reaction encoder, mapping the reaction CLS embedding vector into a reaction query embedding vector through a linear layer corresponding to the reaction encoder, calculating the similarity between the reaction query embedding vector and each protein embedding vector, and sorting the similarity; outputting a protein sequence corresponding to the plurality of protein embedding vectors with the highest similarity; or when the input to-be-queried data is the template enzyme, determining a template enzyme CLS embedding vector by using the protein encoder, mapping the template enzyme CLS embedding vector into a template enzyme query embedding vector through a linear layer corresponding to the protein encoder, calculating the similarity between the template enzyme query embedding vector and each protein embedding vector, and sorting the similarity to obtain the template enzyme query embedding vector. And outputting the protein sequences corresponding to the plurality of protein embedding vectors with the highest similarity.

The invention discloses a soybean GmAGO10 gene and an application of the soybean GmAGO10 gene. Wherein the nucleotide sequence of the soybean GmAGO10 gene is as shown in SEQ ID NO: 3. The invention also relates to a protein coded by the soybean GmAGO10 gene, and the amino acid sequence of the protein is shown as SEQ ID NO: 4. The invention further relates to application of the soybean GmAGO10 gene in cultivation of a single-plant high-yield soybean plant, the GmAGO10 mutant soybean plant shows an obvious phenotype of flowering number increase and branch number increase, and the single-plant yield of the GmAGO10 mutant soybean plant is remarkably improved by 79% compared with that of a wild type.

The invention relates to the technical field of plant genetic engineering, in particular to a ZmbHLH166 gene related to drought resistance in the flowering stage of corn, an encoding protein and application of the ZmbHLH166 gene. The key effect of the ZmbHLH166 gene in the drought resistance of the corn in the flowering period is identified for the first time on the basis of whole genome association analysis (GWAS) and transgenic function verification, and through experiments, through genetic transformation and continuous selfing screening of a Ubi1: ZmbHLH166 overexpression vector, the obtained T2-generation corn strain of the overexpression ZmbHLH166 gene has the advantages that compared with a wild type corn strain, the yield is increased, and the yield is increased. The ZmbHLH166 gene has a shorter pollen scattering-spinning interval, which indicates that under drought stress, the overexpression of the ZmbHLH166 gene can effectively promote the synchronous development of male and female flowers of corn, thereby enhancing the drought resistance of crops. Therefore, the application of the ZmbHLH166 gene and the protein coded by the ZmbHLH166 gene in regulating and controlling the drought resistance of the corn in the flowering period is feasible, and a new and reliable gene resource and an improvement direction are provided for drought-resistant breeding of the corn.

The invention belongs to the technical field of gene engineering, and discloses application of an F-box protein gene OsFBX235 in improvement of bacterial blight resistance. The invention specifically discloses an application of knocking out OsFBX235 gene in improving the bacterial blight resistance of rice or cultivating a bacterial blight resistant rice variety. The F-box protein coding gene OsFBX235 is separated and cloned from rice, and the gene is proved to participate in the defensive reaction of the rice to the bacterial blight for the first time through functional analysis, and is an important negative regulation factor for regulating the resistance of the rice bacterial blight. The gene OsFBX235 is knocked out through target gene screening in combination with a CRISPR / Cas9 technology, and the importance of the gene OsFBX235 on resistance regulation and control of the rice bacterial blight is proved. Meanwhile, knockout of the gene can be used for creating rice germplasm resources with high bacterial leaf blight resistance and can be applied to breeding of new varieties with the bacterial leaf blight resistance of the rice.

The invention relates to the technical field of animal genetic breeding and genomics, and discloses a reference genome of a chromosome level of a Zhaotong Wujin pig and application of the reference genome. The high-quality chromosome level reference genome of the Zhaotong Wujin pig is constructed for the first time. The total length of the genome is about 2.66 Gb, the chromosome anchoring rate is more than 98%, Contig N50 is about 102Mb, and the integrity evaluation of the genome is more than 98%; 21507 protein coding genes are annotated in total. Compared with the existing reference sequence, the genome disclosed by the invention contains specific sequence variation and gene family information of local pig breeds, and provides a more accurate and comprehensive genetic blueprint. The genome resource can be widely applied to molecular mechanism research related to traits such as meat quality, reproductive capacity, growth speed, disease resistance and the like, molecular marker development, breeding chip design, quantitative trait locus (QTL) positioning, genetic evaluation, gene editing, transgenic breeding, germplasm resource protection and the like. The method has important application value in genetic improvement of Chinese local pig breeds.

The invention discloses a wheat heat-resistant protein TaHT-H8 as well as a coding gene and application thereof. The invention provides a protein TaHT-H8, a substance for up-regulating, enhancing or improving the expression of a coding nucleic acid of the protein TaHT-H8, or any one of the following applications of a substance for up-regulating, enhancing or improving the activity or content of the protein TaHT-H8: A1) improving the heat resistance of a plant; the heat resistance of the plant with the TaHT-H8 protein coding gene overexpressed is obviously higher than that of a wild type plant, and the TaHT-H8 protein coding gene can be used for industrial production and plant breeding.

The invention provides a recombinant herpes simplex virus and a herpes simplex virus modification method. The herpes simplex virus modification method comprises the step of replacing an ICP4 protein coding gene in a herpes simplex virus genome containing an infected cell protein 4 (ICP4) gene with an hTERTp-fluorescent protein expression cassette. The expression cassette comprises an hTERTp promoter and a fluorescent protein coding sequence controlled by the hTERTp promoter, and the transcription direction is opposite to that of an ICP4 promoter in a genome. The hTERTp-fluorescent protein expression cassette in the recombinant virus obtained by the method disclosed by the invention can be normally expressed in response to telomerase activity. Therefore, the recombinant virus is capable of expressing a fluorescent protein, such as mBaoJin, in a cell having human telomerase activity. Cells infected by the virus can be identified through fluorescence signals, and the higher the telomerase activity is, the stronger the fluorescence intensity is. The virus has wide application value in research of tumor action mechanisms and stem cell action mechanisms, health assessment, screening of tumor drugs, research and development of diagnostic reagents and establishment of animal models.

The present application relates to a kind of cytochrome P450 oxidase CYP716C52 protein, and the CYP716C52 protein coding gene, the protein can catalyze hydroxylation of maytenic acid C2 position to generate triptolide acid C, in turn participate in triptolide biosynthesis.

The present disclosure generally relates to devices, compositions and methods for designing and producing nucleic acid molecules and the production of encoded proteins using these nucleic acid molecules. In some aspect, the disclosure relates to automation for the in vitro generation of coding DNA molecules, the in vitro transcription of these DNA molecules to generate protein coding RNA molecules, and the in vitro translation of these protein coding RNA molecules to produce proteins.

The invention discloses a rice laodelphax striatellus resistance gene OsSAMS1 and application thereof. The gene provided by the invention is a DNA (Deoxyribonucleic Acid) molecule as shown in SEQ ID NO.2 and SEQ ID NO.3. The invention also provides a method for preparing the Corresponding proteins coded by the gene provided by the invention are proteins as shown in (a) and (b): (a) an amino acid sequence as shown in SEQ ID NO.1; and (b) a protein which is formed by substitution and / or deletion and / or addition of one or more amino acid residues of the amino acid sequence shown in SEQ ID NO.1, is related to rice laodelphax striatellus resistance and is derived from SEQ ID NO.1. The rice insect-resistant related protein provided by the invention affects the insect resistance of plants. Insect-sensitive plants can resist insects by improving expression of the protein coding gene, and laodelphax striatellus-resistant transgenic plants can be cultivated by silencing the gene. The gene and the encoding protein thereof can be applied to plant genetic improvement.

The present invention relates to a rep plasmid comprising at least one long adeno-associated virus replication protein coding sequence encoding at least one long functional rep protein, at least one short adeno-associated virus replication protein coding sequence encoding at least one short functional rep protein, and at least one heterologous element, wherein following translation the at least one heterologous element results in a ratio of long adeno-associated virus rep protein to short adeno-associated virus rep protein of about 1:5 to about 1:5000.

The invention relates to the technical field of biology, in particular to application of an RhUNE10 gene to regulation and control of root cancer resistance of Chinese roses and a regulation and control method. The nucleotide sequence of the RhUNE10 gene is as shown in SEQ ID NO. 1. The application mode is that the RhUNE10 gene is silenced and knocked out, or the expression of the protein coded by the RhUNE10 gene is inhibited; and the amino acid sequence of the protein coded by the RhUNE10 gene is as shown in SEQ ID NO. 3. After the RhUNE10 gene is silenced, the resistance of the Chinese rose to agrobacterium tumefaciens is remarkably reduced, which indicates that the RhUNE10 gene has important biological functions, is beneficial to promoting Chinese rose functional genomics research, gene editing breeding and transgenic breeding research, excavates and cultivates new varieties of high-resistance Chinese roses, and has wide application prospects. And a certain theoretical basis and key gene resources are provided for comprehensive prevention and treatment of root cancer and cultivation of a new variety of Chinese rose.

The invention discloses application of a lycium barbarum Lb03G03224 gene in catalysis of esterification of free carotenoids, the nucleotide sequence of the lycium barbarum Lb03G03224 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of encoded protein is as shown in SEQ ID NO: 2. According to the present invention, it is found that the zeaxanthin dipalmitate and the isomer 9 (Z)-zeaxanthin dipalmitate thereof can be generated when the Lb03G03224 gene is expressed in the engineering escherichia coli for generating beta-carotene, beta-cryptoxanthin and zeaxanthin, such that the zeaxanthin dipalmitate and the isomer 9 (Z)-zeaxanthin dipalmitate can be produced, the protein coded by the Lb03G03224 has the function of catalyzing esterification reaction of carotenoid containing free hydroxyl and fatty acid acyl donor, and has important application value in in-vitro biosynthesis of esterified carotenoid.

The invention belongs to the field of biotechnology engineering, and particularly relates to application of a tomato SlCOMT1 gene in regulation and control of tomato anther heat resistance. The invention discloses a nucleotide sequence SEQ ID NO.1 of a tomato SlCOMT1 gene and an amino acid sequence SEQ ID NO.2 of a protein coded by the gene, a slcomt1 knockout mutant plant is obtained by utilizing a CRISPR-Cas9 technology, and under high temperature stress, compared with a wild plant, the tomato pollen viability and the fruit setting rate of the SlCOMT1 knockout mutant plant are remarkably improved. The invention proves that SlCOMT1 mutation can cause accumulation and increase of endogenous 5-hydroxytryptamine in tomato flowers at high temperature, so that the high-temperature resistance of tomato anther development is improved, and the yield of tomato anther is stabilized. According to the method, the new germplasm of the heat-resistant tomato can be quickly created, support is provided for high-temperature-resistant production of the tomato, and the method has great application value.

The invention discloses a gene for promoting low-nitrogen efficient utilization of rice and enlarging grains and application of the gene. The gene is derived from rice and is named as OsUGT, and the nucleotide sequence of the gene is shown as SEQ ID NO.1; the amino acid sequence of the protein coded by the gene is as shown in SEQ ID NO. 2. The invention provides a construction method of an OsUGT gene overexpressed transgenic rice strain, which comprises the following steps: constructing pRHVc-OsUGT recombinant plasmids, transforming the pRHVc-OsUGT recombinant plasmids into calluses through an agrobacterium tumefaciens-mediated method, and screening resistant calluses and regenerating plants to obtain the OsUGT gene overexpressed rice. The OsUGT gene is overexpressed in a rice plant by adopting a pRHVc vector through a transgenic technology, so that the nitrogen utilization efficiency of the plant in a medium-low nitrogen stress environment can be remarkably improved, grain enlargement and grain weight increase can be effectively promoted, stable improvement of the crop yield under a low-nitrogen condition is realized, and the technical effect is clear and efficient.

The invention belongs to the technical field of gene engineering, and discloses a glomus huanghuai EPI7 protein, a coding gene and application thereof, the amino acid sequence of the glomus huanghuai EPI7 protein is shown as SEQ ID NO.2, and the nucleotide sequence of nucleic acid or the gene for coding the EPI7 protein is shown as SEQ ID NO.1. The invention further discloses a preparation method of the glomus huanghuai EPI7 protein. The invention proves that the glomus huanghuai EPI7 protein plays an important role in the process of improving the disease resistance of plants through cloning of the glomus huanghuai EPI7 protein gene and transient expression of tobacco.

The invention provides a method for producing L-carnosine, a genetic engineering strain as well as a preparation method and application of the genetic engineering strain, and belongs to the field of genetic engineering. According to the genetic engineering strain provided by the invention, by knocking out a glucose-6-phosphate isomerase coding gene pgi, knocking out an L-threonine / L-homoserine transporter coding gene rhtA or knocking out a dipeptide transfer protein coding gene dppABCD, the yield of extracellular L-carnosine is increased, the shake flask yield of the finally constructed strain reaches 9.1 g / L, the 5L fermentation tank yield reaches 65 g / L, and the yield of the extracellular L-carnosine reaches 9.1 g / L; and the method has a good application prospect in L-carnosine biosynthesis.

The invention belongs to the technical field of plant molecular biology, and discloses application of a soybean GmBZL gene in reduction of soybean grain weight, plant height, oil content and / or improvement of protein content. The soybean GmBZL gene comprises a soybean GmBZL3 gene of which the CDS sequence is as shown in SEQ ID NO: 1 and / or a soybean GmBZL4 gene of which the CDS sequence is as shown in SEQ ID NO: 2. The GmBZL gene is a key gene for regulating and controlling the grain weight, the oil content, the protein content and the plant height of soybeans, valuable gene resources are provided for crop breeding, the soybean GmBZL gene can be widely applied to soybean breeding, and short-plant soybeans with small grains, high protein content and low oil content are obtained.

The application discloses a sequence of a separated new bunyavirus M segment and a Gn protein coded by the sequence, a nucleotide sequence of the M segment and an amino acid sequence coded by the nucleotide sequence, and expression, purification and activity analysis of a fusion protein formed by fusing the Gn coded by the M segment with Fc to form a Gn-Fc protein. In addition, the application also relates to cross reaction of the fusion protein with polyclonal antibodies in different patient sera.

The invention discloses a novel polypeptide with sweet potato salt tolerance regulation activity and derived from sweet potatoes. The amino acid sequence of protein coded by the novel polypeptide is AGPSKSGKGGKKRR. Functional verification shows that the salt tolerance of the sweet potatoes can be remarkably improved by exogenous application, so that the growth and development of the sweet potatoes are promoted in a salt stress environment, and the photosynthetic efficiency is improved. The novel polypeptide provided by the invention can be used for cultivating a new sweet potato variety with enhanced salt tolerance, and has important application value for expanding a sweet potato planting area and improving the utilization rate of saline-alkali soil.

The invention discloses a rice fork head related structural domain protein coding gene OsFHA11 and application thereof, and belongs to the technical field of plant genetic engineering. According to the invention, the OsFHA11 gene is connected with an overexpression vector through a genetic engineering means and then is transformed into rice, so that the expression of the gene in the transformed rice plant is improved, and the resistance of the rice plant to sheath blight can be improved. Through cloning of the OsFHA11 gene and analysis of the sheath blight resistance function of the OsFHA11 gene, analysis of a molecular mechanism of rice sheath blight resistance is facilitated. In practice, a sheath blight resistant rice material can be cultivated by using the gene through a transgenic means, and a sheath blight resistant rice variety with stable characters is further obtained through interspecies hybridization, so that the occurrence of sheath blight is effectively controlled, and the rice yield is increased.

The application discloses application of a rice metal tolerance protein coding gene OsMTP7 The rice metal tolerance protein coding gene OsMTP7 has the accession number of AK241225.1 in Genbank. The gene coding protein has zinc transport activity, can increase the zinc content of rice root system and above-ground part, and significantly increases the accumulation of zinc in rice grains.

The application discloses application of ZmSAUR72 protein and a coding gene thereof in regulation of drought resistance of plants and belongs to the technical field of genetic engineering breeding. The application discloses application of the ZmSAUR72 protein or an expression material for regulating the coding gene of the protein or a material for regulating the activity or content of the protein in regulation of drought resistance of plants. The amino acid sequence of the protein is shown as SEQ ID No. 1, the nucleotide sequence of the coding gene of the protein is shown as SEQ ID No. 1, and it is verified that the regulation in the above application is positive regulation. By increasing the expression amount and / or activity of the ZmSAUR72 protein, the interval of corn loose spinning time is reduced, the drought resistance of plants is enhanced, the grain number of a single ear of plants is increased, and the yield of a single ear of plants is increased. The negative regulation effect of inhibition or reduction of the expression amount of the ZmSAUR72 gene in the above application is also verified by a gene knockout method. The application further discloses a method for improving the drought resistance of plants, which comprises introducing the coding gene of the protein into a receptor plant to obtain a plant with improved drought resistance.

The invention belongs to the technical field of molecular biology breeding, and particularly relates to application of nucleoside diphosphate kinase GhNDPK9 in promoting salt tolerance of cotton. The amino acid sequence of the protein coded by the GhNDPK9 gene is as shown in SEQ ID NO. 2. The invention discloses the effect of the GhNDPK9 gene in positive regulation and control of the salt tolerance of the cotton, enriches functional genes for salt-tolerant cotton breeding, and provides a new effective way for genetic improvement of salt stress resistance of the cotton. By means of transgenic genetic breeding, the expression level of the GhNDPK9 gene is improved, damage symptoms of plants under the salt stress condition are relieved, the salt tolerance of the plants is enhanced, and then the viability of cotton on salinized soil is improved; the method has wide application prospects and good social benefits in the aspects of genetic improvement of salt-tolerant new germplasm, widening of the suitable planting range of cotton and the like.

The application discloses application of a CsERF017 gene or a protein coded by the same in regulation of resistance of navel orange fruits to sour rot, and belongs to the technical field of molecular biology and fruit and vegetable preservation. A nucleotide sequence of the CsERF017 gene is shown in SEQ ID NO. 2, and an amino acid sequence of the protein coded by the same is shown in SEQ ID NO. 3. The application finds through experiments that the CsERF017 gene is up-regulated after infection of a citrus sour rot pathogen Geotrichum candidum, and silencing or knocking out the CsERF017 gene can enhance the resistance of navel orange fruits to sour rot, and the specific performance is that, compared with a control, the transgenic fruits after inoculation of the G. candidum have reduced symptoms of sour rot, and both a disease index and a TA content are reduced. The application provides an important gene bank and new insights for breeding of citrus disease-resistant varieties and development of molecular markers, and is helpful to fundamentally reduce the dependence on chemical synthetic pesticides in postharvest preservation treatment of citrus fruits.

The Quadruplet Expanded DNA (QED) eukaryote genetic code comprising twenty nondegenerate QED codons encode proteins (the protein-encoding codons), and thirty-five nondegenerate QED codons (the noncoding codons) being highly correlated with cis-regulatory elements control and regulate transcription, alternate splicing, and polymerization in eukaryotic protein synthesis using canonical amino acids. The QED eukaryote genetic code is an advancement to gene therapeutics that allows for the correction of dysfunctional proteins. Additionally, the QED eukaryote genetic code is further applicable for changing paradigms relating to identifying cures for monogenic rare, multigene cancer, and neurodegenerative diseases.

The invention provides a peanut bacterial wilt resistance NBS-LRR coding gene AhRRS3 and application thereof. The nucleotide sequence of the gene AhRRS3 is shown as SEQ ID NO.1, and the amino acid sequence of the protein coded by the gene AhRRS3 is shown as SEQ ID NO.2. The invention further provides a preparation method of the peanut bacterial wilt resistance NBS-LRR coding gene AhRRS3. According to the invention, an overexpression vector of AhRRS3 is constructed, arabidopsis thaliana is transformed, and the disease-resistant function of the arabidopsis thaliana is observed. Research results show that after being infected by ralstonia solanacearum, the disease-resistant variety of arabidopsis thaliana and a disease-susceptible strain of the overexpressed gene AhRRS3 show obvious disease resistance, the disease-susceptible strain is in a whole-plant withering and death state, and the results show that the overexpressed gene AhRRS3 can improve the bacterial wilt resistance of the plant. The invention provides high-quality gene resources and theoretical basis support for genetic engineering breeding of plant bacterial wilt resistance.