497 results about "Protein coding" patented technology

The invention relates to the field of artificial intelligence assisted drug discovery, in particular to a protein large language model-based molecule generation and optimization method, which comprises the following steps of: acquiring amino acid sequence information and three-dimensional structure information of a target protein pocket; encoding the amino acid sequence of the protein pocket by using a protein encoder constructed based on a protein large language model to obtain a protein pocket feature vector; using a context encoder module to encode the context information according to a preset molecule generation mode (de novo generation or optimization based on a seed compound) to obtain a latent vector; and fusing the protein pocket feature vector with the latent vector. According to the method, accurate representation of the protein pocket is realized by utilizing the protein large language model, and a generation-screening-optimization iterative drug design strategy is developed by supporting a unified framework of two generation modes, so that the targeting specificity of generated molecules and the overall efficiency of drug design are improved.

The invention discloses a SbWRKY51 gene, a promoter and application of the SbWRKY51 gene and the promoter in improvement of salt tolerance of sorghum, and belongs to the technical field of plant genetic engineering. The SbWRKY51 gene is screened from sorghum, and the expression level of the SbWRKY51 gene is up-regulated after salt stress treatment. The protein coded by the gene belongs to a WRKY transcription factor family, has transcriptional activation activity, and can start the expression of a reporter gene. Sorghum overexpression strains and complementary strains of the gene are obtained, salt tolerance related physiological indexes are measured, overexpression of the gene can improve the seed germination rate, increase the seed root length, regulate ion balance and enhance the free radical scavenging capacity of plants, and the effect of the gene in the aspect of improving the salt tolerance is embodied. A further research finds that the SbWRKY51 gene can enhance the plant salt tolerance by regulating a lignin synthesis pathway. The technical scheme provides a basis for cultivating resistant plants, and has important guiding significance for enhancing the production potential of sorghum under a high-salt condition and promoting agricultural development.

The invention discloses application of wheat Rht1-D1b protein in regulation and control of a wheat tillering angle, and belongs to the technical field of biological breeding. The technical problem to be solved by the invention is how to increase the tillering angle of plants. Therefore, the invention provides the application of the protein or a substance for regulating and controlling the expression of a protein coding gene or a substance for regulating and controlling the activity or content of the protein in regulating and controlling the tillering angle of the plant, wherein the protein can be Rht1-D1b protein; the Rht1-D1b protein can be a protein of which the amino acid sequence is as shown in SEQ ID No. 3. According to the application, the Rht1-D1b gene is finally localized through gene localization and map-based cloning. The gene significantly increases the tillering angle of wheat. The wheat gene Rht1-D1b can be widely applied to the plant fields of wheat germplasm resource improvement, wheat plant type genetic breeding and the like, and has an important application value for improving the wheat yield.

The invention relates to the technical field of biology, in particular to a vitis davidii VdYUCCA10 gene as well as an encoding protein and application thereof. The vitis davidii VdYUCCA10 gene is located on a chromosome 7 of vitis davidii and is distributed in a 2271185-2274940 region, and the nucleotide sequence of the vitis davidii VdYUCCA10 gene is shown as SEQ ID NO. 1. The amino acid sequence of the protein coded by the gene is as shown in SEQ ID No.2. The invention also discloses an application of the vitis davidii VdYUCCA10 gene in enhancing the resistance of grape fruits to pathogenic bacteria after silencing. After the VdYUCCA10 gene provided by the invention is silenced, the resistance of grapes to anthracnose can be improved, and a theoretical basis is provided for grape anthracnose resistance breeding.

The invention discloses an application of a CsESE3 gene or a protein coded by the CsESE3 gene in regulation and control of plant shoot development, and belongs to the technical field of plant genetic engineering. Traditional citrus crossbreeding is long in period of cultivating ideal plant type plants, low in selection efficiency and few in selectable cultivation characters. Manual shoot control is high in labor cost, and chemical shoot control has the risk of phytotoxicity. Aiming at the technical problems, the invention provides a new scheme for regulating and controlling the development of citrus branch tips based on CsESE3 gene expression: the development of the branch tips is obviously promoted after the expression of the CsESE3 gene is improved, so that the CsESE3 can become a target spot for regulating and controlling the development of the citrus branch tips.

The invention relates to the technical field of mRNA vaccines, and discloses a feline infectious peritonitis mRNA vaccine as well as a preparation method and application thereof. The feline infectious peritonitis mRNA vaccine comprises mRNA molecules; the mRNA molecule comprises an FIBV-S protein coding sequence as shown in SEQ ID NO: 6. According to the invention, the mRNA sequence for coding the natural FIBV-S protein is modified, so that the FIBV-S protein obtained by translation can be stably maintained at the prefusion conformation and has higher immunogenicity, thereby endowing the mRNA vaccine with a better immune effect and being beneficial to the prevention of feline infectious peritonitis.

The invention provides a system and a method for screening Cas protein mutants capable of identifying different PAM sequences, and belongs to the technical field of bioengineering. The system comprises a Cas protein mutant screening vector and an sgRNA expression vector, the Cas protein mutant screening vector comprises the following expression elements: a Cas protein coding gene, a plasmid replicon and a first resistance screening tag expression cassette; the sgRNA expression vector comprises the following expression elements: a plasmid replicon, a second resistance screening tag expression cassette, a third resistance screening tag expression cassette and an sgRNA expression cassette. According to the system and the method, aiming at the problem that the efficiency of identifying PAM sequences except NGG by wild type spCas9 protein is low, an active Cas9 mutant capable of identifying PAM sites except NGG is screened out, and the application field and the editing efficiency of a CRISPR gene editing system can be greatly expanded.

The invention provides application of an OsRTD1 gene in improving drought resistance of rice, and belongs to the technical field of gene engineering and rice drought resistance. The nucleotide sequence of the OsRTD1 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the protein coded by the OsRTD1 gene is as shown in SEQ ID NO: 2. Compared with a wild plant, the survival rate of the OsRTD1 gene mutant plant osrtd1 under the drought stress of PEG induction and soil dehydration is remarkably improved, the survival rate of the OsRTD1 gene overexpression plant OsRTD1-OE under the drought stress is remarkably reduced, the relative water loss rate of dehydrated leaves is higher, and the survival rate of the OsRTD1 gene overexpression plant OsRTD1-OE under the drought stress is remarkably reduced. The total grain number, the solid grain number, the total grain weight and the like of the OsRTD1 gene mutant plant osrtd1 under the stress of field drought are obviously higher than those of a wild plant. The OsRTD1 gene is a potential candidate new target for regulating and controlling a rice drought resistance mechanism.

The invention belongs to the technical field of molecular biology breeding, and particularly relates to application of a GhDCP5L gene in creation of high-temperature response cotton germplasm, and an amino acid sequence of a protein coded by the GhDCP5L or a homologous gene thereof is as shown in SEQ ID NO.2 or SEQ ID NO.4. The invention provides a novel thermal response gene, a gene resource library of high-temperature response of cotton is enriched, the GhDCP5L gene and a homologous gene thereof are applied to plant genetic engineering breeding, and new germplasm of high-temperature-resistant or sensitive high-temperature-resistant cotton can be accurately created by regulating and controlling the expression of the gene, so that the high-temperature-resistant or sensitive high-temperature-resistant cotton is obtained. The method provides technical support for coping with stable yield of cotton under the background of global warming climate, can be used for preparing male sterile line cotton germplasm by performing high-temperature treatment on the high-temperature-sensitive material, has good application value in the aspects of cotton hybrid seed production, promotion of hybrid cotton development and the like, and is beneficial to improving the economic benefit of the cotton industry.

The invention provides a method and a device for designing a protein coding codon sequence based on a large multi-modal model, which comprehensively utilize information such as an amino acid sequence, space folding and mRNA quality of protein to realize high-quality, controllable and evaluable codon sequence design. According to the method, the dependence of a traditional codon optimization strategy on a reference gene template is broken through, and a codon optimization problem is converted into a codon design task based on protein information. Through comprehensive consideration of information such as space folding of protein, context of amino acid sequences, mRNA quality and the like, the model can generate a codon sequence which is more in line with codon use preference of specific species, reasonable in structure and efficient in expression, so that a new thought and technical support are provided for safe, stable, efficient and controllable protein expression.

The invention discloses a SiERF109 gene for regulating drought resistance, salt tolerance and flavonoid accumulation of millet and application of the SiERF109 gene, and belongs to the technical field of plant breeding. According to the invention, a gene SiERF109 with a nucleotide sequence shown as SEQ ID NO.1 is separated and cloned from millet, and fluorescent quantitative expression analysis shows that SiERF109 participates in drought and salt stress response of millet. After the gene is over-expressed in foxtail millet, in drought and salt stress experiments, compared with a wild type strain, the survival rate and chlorophyll content of a strain over-expressed with the SiERF109 gene are remarkably increased, the flavonoid content is increased by 33.5%-39.5%, and active oxygen accumulation is remarkably reduced; it is shown that the SiERF109 gene and the protein coded by the SiERF109 gene can remarkably improve the drought resistance, the salt resistance and the flavonoid content of the plant.

The invention relates to the technical field of biology, in particular to a vitis davidii VdbHLH51 gene as well as an encoding protein and application of the vitis davidii VdbHLH51 gene. The vitis davidii VdbHLH51 gene is located on a chromosome 13 of vitis davidii and is distributed in a 772522-773497 region, and the nucleotide sequence of the vitis davidii VdbHLH51 gene is as shown in SEQ ID NO. 1. The amino acid sequence of the protein coded by the gene is as shown in SEQ ID No.2. The invention also discloses an application of the vitis davidii VdbHLH51 gene in improvement of resistance of grapes to pathogenic bacteria after overexpression of grape calluses. The VdbHLH51 gene provided by the invention can improve the resistance of grapes to grape anthracnose, and provides a theoretical basis for grape anthracnose resistance breeding.

The present disclosure, among other things, provides machine-learning technologies for identifying and localizing particular genomic elements (e.g., gene elements and / or regulatory elements) within nucleotide sequences, such as DNA and / or RNA sequences. In certain embodiments, similar to the manner in which image processing methods can be used to localize particular objects in images at pixel level resolution, referred to as “segmentation,” systems and methods of the present disclosure predict presence and locations of certain genomic elements within nucleotide sequences, thereby “segmenting” nucleotide sequences. Accordingly, genomic element segmentation technologies described herein may be used to generate annotations that identify and label portions of nucleotide sequences according to their predicted (e.g., via machine learning models described herein) function—e.g., as protein-coding genes, untranslated regions, splice sites, promotors, enhancers, etc. Among other things, these genomic annotations may be used to inform underlying biological processes driving diseases and facilitate development of new therapies.

The invention belongs to the field of gene engineering, and relates to application of a soybean saline-alkaline tolerant gene GmANN13. The soybean annexin gene GmANN13 is applied to gene engineering for improving the saline-alkaline tolerance of soybeans. The invention discloses application of the soybean annexin gene GmANN13 in cultivation of saline-alkaline tolerant soybean varieties. The invention also discloses application of an expression vector containing the soybean annexin gene GmANN13 in cultivation of saline-alkaline tolerant soybean varieties. Stable transformation and saline-alkaline tolerance researches of soybean hairy roots, arabidopsis thaliana and soybeans show that the soybean GmANN13 responds to induction of saline-alkaline stress, and the saline-alkaline tolerance of plants can be improved by overexpressing the gene in the soybeans. Therefore, when the plant saline-alkaline tolerance related protein coding gene GmANN13 is transferred into crops through a genetic engineering means, a new variety of transgenic plants with saline-alkaline stress tolerance can be obtained.

The disclosure relates to compositions and methods for treating a disease or condition associated with a TDP-pathology or a decline in TDP-43 functionality in neuronal cells in a subject, and for identifying candidate agents to restore expression of a normal full-length or protein coding STMN2 RNA.

The invention discloses an application of an OsSPL14 gene and a protein coded by the OsSPL14 gene in regulating and controlling the diameter of a plant root system, and belongs to the technical field of gene engineering. The technical problem to be solved by the invention is how to regulate and control the root diameter of a plant. In order to solve the technical problem, the invention provides application of the protein or a substance for regulating and controlling the expression of a protein coding gene or a substance for regulating and controlling the activity or content of the protein in regulating and controlling the diameter of a plant root system. The protein has an amino acid sequence of sequence 2. The invention discloses that OsSPL14 is an important gene for regulating and controlling the diameter of the rice root for the first time. The expression of the OsSPL14 is driven by using a specific promoter, so that the regulation effect of the OsSPL14 in a rice root system can be specifically exerted, and the diameter of the adventitious root of the rice is increased. The OsSPL14 provided by the invention has important significance in cultivating a new rice variety with high and stable yield, high quality and good stress resistance.

The invention discloses a BvM14-TAG2 gene and application thereof, and relates to the technical field of biological genes. The CDS sequence of the BvM14-TAG2 gene disclosed by the invention is as shown in SEQ ID NO. 1. The amino acid sequence of the protein coded by the BvM14-TAG2 gene is as shown in SEQ ID No. 2. According to the invention, the BvM14-TAG2 gene is utilized to construct an arabidopsis thaliana plant for heterologous expression of the BvM14-TAG2 gene, stress treatment is carried out, and the seed germination rate and root length of the plant for heterologous expression of the BvM14-TAG2 gene are both superior to those of a wild type; the biomass of a plant for heterologous expression of the BvM14-TAG2 gene is higher than that of a wild plant; the result shows that the BvM14-TAG2 gene can promote the growth and development of the plant under the salt stress, and can improve the adaptive capacity of the BvM14-TAG2 transgenic plant under the salt stress.

The invention discloses a cherry sugar transporter gene CpSWEET10 and application thereof, and belongs to the technical field of cherry gene engineering. A novel sugar transporter gene CpSWEET10 is obtained through cloning, the nucleotide sequence of the novel sugar transporter gene CpSWEET10 is shown as SEQ ID NO.1, and the coded amino acid sequence of the novel sugar transporter gene CpSWEET10 is shown as SEQ ID NO.2. The gene is highly expressed in cherry fruits, and the content of soluble solids and total sugar in mature fruits is remarkably positively correlated with the expression quantity of the soluble solids and the total sugar; through subcellular localization and sugar transport function defect type yeast mutant functional complementation experimental analysis, the protein coded by the CpSWEET10 gene is localized in a cytoplasmic membrane and has a sucrose transport function; the cherry sugar transporter gene CpSWEET10 disclosed by the invention can be applied to the improvement of the sugar degree character of the fruits, and a new gene resource is provided for molecular breeding for improving the high-sugar quality of the cherry fruits.

The invention discloses a salt stress resistant gene BvAGER1 and application thereof, and relates to the technical field of biological genes, in particular to the salt stress resistant gene BvAGER1 and the application field thereof. According to the salt stress resistant gene BvAGER1 disclosed by the invention, a CDS (Coding Sequence) sequence of the gene BvAGER1 is as shown in SEQ ID NO. 1. The amino acid sequence of the protein coded by the salt stress resistant gene BvAGER1 is as shown in SEQ ID No. 2. The invention also discloses an application of the salt stress resistant gene BvAGER1 in improving the salt tolerance of plants. The BvAGER1 gene in a BvAGER1 gene heterologous overexpression plant prepared by utilizing the BvAGER1 gene can be stably inherited, the heterologous expression BvAGER1 gene can relieve the inhibition effect of salt stress on plant germination, and the inhibition effect on growth of roots and leaves of the BvAGER1 gene heterologous overexpression plant can be obviously weakened under the salt stress; the adaptive capacity under the salt stress is improved.

The invention discloses a sugarcane ScPP2C49 gene and application thereof, and relates to the technical field of plant biology, the nucleotide sequence of the ScPP2C49 gene is as shown in SEQ ID No.1, and the amino acid sequence of protein coded by the ScPP2C49 gene is as shown in SEQ ID No.2. The gene is induced to express by drought and abscisic acid, and the encoded protein of the gene is positioned in a cell nucleus. Functional studies show that after overexpression of the ScPP2C49 gene in the plant, the drought resistance of the plant is negatively regulated through various mechanisms of inhibiting an abscisic acid signal channel, hindering stomatal closure under drought stress, weakening active oxygen scavenging ability, reducing photosynthetic efficiency, inhibiting root growth and the like. The invention also provides a recombinant overexpression vector containing the gene and a method for obtaining a transgenic plant with reduced drought resistance by using the vector through an agrobacterium-mediated method. According to the invention, a new gene resource is provided for deep analysis of a plant drought-resistant molecular mechanism, and an important target gene is provided for cultivation of high-stress-resistance crop varieties through a reverse genetics means (such as gene knockout).

The invention provides an enzyme mining method, system and medium. The enzyme mining method comprises the following steps: inputting to-be-queried data; inputting the data to be queried into a pre-trained VenusRXN model; when the input to-be-queried data is a reaction, determining a reaction CLS embedding vector by using a reaction encoder, mapping the reaction CLS embedding vector into a reaction query embedding vector through a linear layer corresponding to the reaction encoder, calculating the similarity between the reaction query embedding vector and each protein embedding vector, and sorting the similarity; outputting a protein sequence corresponding to the plurality of protein embedding vectors with the highest similarity; or when the input to-be-queried data is the template enzyme, determining a template enzyme CLS embedding vector by using the protein encoder, mapping the template enzyme CLS embedding vector into a template enzyme query embedding vector through a linear layer corresponding to the protein encoder, calculating the similarity between the template enzyme query embedding vector and each protein embedding vector, and sorting the similarity to obtain the template enzyme query embedding vector. And outputting the protein sequences corresponding to the plurality of protein embedding vectors with the highest similarity.

The application discloses an elagatis bipinnulatus IGF2 gene and application thereof, wherein the nucleotide sequence of the elagatis bipinnulatus IGF2 gene is shown as SEQ ID NO:1, the amino acid sequence of the protein coded by the IGF2 gene is shown as SEQ ID NO:2. A prokaryotic expression vector containing the gene and a corresponding recombinant strain are successfully constructed, a recombinant IGF2 protein is prepared through a high-efficiency expression system, and the recombinant IGF2 protein is applied to preparation of a product for regulating fish feeding, preparation of an additive with the function of promoting fish feeding and preparation of artificial feed with the function of promoting fish feeding. The recombinant IGF2 protein can significantly enhance the expression of feeding-related genes of the elagatis bipinnulatus and significantly improve the feeding amount.

The invention discloses a soybean GmAGO10 gene and an application of the soybean GmAGO10 gene. Wherein the nucleotide sequence of the soybean GmAGO10 gene is as shown in SEQ ID NO: 3. The invention also relates to a protein coded by the soybean GmAGO10 gene, and the amino acid sequence of the protein is shown as SEQ ID NO: 4. The invention further relates to application of the soybean GmAGO10 gene in cultivation of a single-plant high-yield soybean plant, the GmAGO10 mutant soybean plant shows an obvious phenotype of flowering number increase and branch number increase, and the single-plant yield of the GmAGO10 mutant soybean plant is remarkably improved by 79% compared with that of a wild type.

The invention discloses a gene for degrading chlorophyll as well as an encoding protein and application thereof, and belongs to the fields of molecular biology, plant genetic engineering and horticulture. Protein coded by the gene is located in chloroplast, and chlorophyll degradation can be efficiently promoted under various stress conditions. By performing different treatments on Huangkui tea trees and performing gene function verification in model plants such as nicotiana benthamiana and arabidopsis thaliana, it is found that the CsSGRs gene is subjected to induced expression by factors such as natural aging, abscisic acid, illumination intensity and low temperature, and the gene participates in a chlorophyll degradation approach, so that the functions are redundant. In addition, a bZIP family transcription factor CsABF4 is screened out, and CsSGRs gene expression can be regulated and controlled. The invention provides gene resources and theoretical basis for regulating and controlling the chlorophyll content of plants and improving related characters of plant leaf color, and has potential application value in the fields of plant breeding and gardening.

The invention relates to the technical field of plant genetic engineering, in particular to a ZmbHLH166 gene related to drought resistance in the flowering stage of corn, an encoding protein and application of the ZmbHLH166 gene. The key effect of the ZmbHLH166 gene in the drought resistance of the corn in the flowering period is identified for the first time on the basis of whole genome association analysis (GWAS) and transgenic function verification, and through experiments, through genetic transformation and continuous selfing screening of a Ubi1: ZmbHLH166 overexpression vector, the obtained T2-generation corn strain of the overexpression ZmbHLH166 gene has the advantages that compared with a wild type corn strain, the yield is increased, and the yield is increased. The ZmbHLH166 gene has a shorter pollen scattering-spinning interval, which indicates that under drought stress, the overexpression of the ZmbHLH166 gene can effectively promote the synchronous development of male and female flowers of corn, thereby enhancing the drought resistance of crops. Therefore, the application of the ZmbHLH166 gene and the protein coded by the ZmbHLH166 gene in regulating and controlling the drought resistance of the corn in the flowering period is feasible, and a new and reliable gene resource and an improvement direction are provided for drought-resistant breeding of the corn.

The invention belongs to the technical field of gene engineering, and discloses application of an F-box protein gene OsFBX235 in improvement of bacterial blight resistance. The invention specifically discloses an application of knocking out OsFBX235 gene in improving the bacterial blight resistance of rice or cultivating a bacterial blight resistant rice variety. The F-box protein coding gene OsFBX235 is separated and cloned from rice, and the gene is proved to participate in the defensive reaction of the rice to the bacterial blight for the first time through functional analysis, and is an important negative regulation factor for regulating the resistance of the rice bacterial blight. The gene OsFBX235 is knocked out through target gene screening in combination with a CRISPR / Cas9 technology, and the importance of the gene OsFBX235 on resistance regulation and control of the rice bacterial blight is proved. Meanwhile, knockout of the gene can be used for creating rice germplasm resources with high bacterial leaf blight resistance and can be applied to breeding of new varieties with the bacterial leaf blight resistance of the rice.

The invention relates to the technical field of animal genetic breeding and genomics, and discloses a reference genome of a chromosome level of a Zhaotong Wujin pig and application of the reference genome. The high-quality chromosome level reference genome of the Zhaotong Wujin pig is constructed for the first time. The total length of the genome is about 2.66 Gb, the chromosome anchoring rate is more than 98%, Contig N50 is about 102Mb, and the integrity evaluation of the genome is more than 98%; 21507 protein coding genes are annotated in total. Compared with the existing reference sequence, the genome disclosed by the invention contains specific sequence variation and gene family information of local pig breeds, and provides a more accurate and comprehensive genetic blueprint. The genome resource can be widely applied to molecular mechanism research related to traits such as meat quality, reproductive capacity, growth speed, disease resistance and the like, molecular marker development, breeding chip design, quantitative trait locus (QTL) positioning, genetic evaluation, gene editing, transgenic breeding, germplasm resource protection and the like. The method has important application value in genetic improvement of Chinese local pig breeds.

The invention relates to a recombinant human 2Ig-B7-H3 protein coding gene, a recombinant vector, a host cell, a pharmaceutical composition and application of the recombinant human 2Ig-B7-H3 protein coding gene. The recombinant human 2Ig-B7-H3 protein coding gene comprises a nucleotide sequence with the length of 3095bp, wherein a basic group C at the 1722nd site is replaced by T at a fixed point. The medicine prepared by combining the recombinant human 2Ig-B7-H3 protein coding gene packaged by the lentiviral vector and the wild type human 2Ig-B7-H3 protein coding gene packaged by the lentiviral vector according to a certain proportion has an unexpected effect in the aspect of treating or preventing cancers.

The invention discloses a wheat heat-resistant protein TaHT-H8 as well as a coding gene and application thereof. The invention provides a protein TaHT-H8, a substance for up-regulating, enhancing or improving the expression of a coding nucleic acid of the protein TaHT-H8, or any one of the following applications of a substance for up-regulating, enhancing or improving the activity or content of the protein TaHT-H8: A1) improving the heat resistance of a plant; the heat resistance of the plant with the TaHT-H8 protein coding gene overexpressed is obviously higher than that of a wild type plant, and the TaHT-H8 protein coding gene can be used for industrial production and plant breeding.

This application discloses the application of the MsDGK5 gene and its encoded protein in the salt tolerance of alfalfa plants, belonging to the field of plant genetic engineering technology. The nucleotide sequence of the alfalfa MsDGK5 gene provided in this application is shown in SEQ ID NO.1. This application constructed Arabidopsis and alfalfa transgenic plants overexpressing the MsDGK5 gene, and systematically measured the growth and development indicators and physiological and biochemical parameters of the plants under salt stress, confirming that overexpression of the MsDGK5 gene can significantly improve the salt tolerance of transgenic plants. Based on the above experimental results, this application clarifies for the first time the key regulatory role of the MsDGK5 gene in plant salt tolerance, providing important gene resources for the genetic improvement of crop salt tolerance, and also providing a new technical approach to address the increasingly serious problem of soil salinization.