334 results about "Genetic marker" patented technology

The invention provides a crop whole genome prediction method and system based on machine learning, and the method comprises the steps: firstly obtaining a gene expression data set of a target crop in a plurality of growth cycles, the gene expression data set containing a plurality of gene sequence samples composed of genetic markers and phenotypic character data; performing feature extraction on the gene expression data set to obtain gene association features and growth character features, performing fusion prediction on the gene association features and the growth character features by using a preset machine learning model to generate fusion prediction features, and determining a whole genome prediction result according to the fusion prediction features. The whole genome prediction result can indicate character expression trends of crops in different environments, and finally, an adaptability optimization strategy is generated based on the whole genome prediction result and fed back to a crop cultivation system, cultivation parameter adjustment is triggered, crop cultivation precision and intelligence are achieved, and the cultivation efficiency and crop adaptability are improved.

The invention belongs to the field of SNP (Single Nucleotide Polymorphism) detection, and particularly provides an SNP molecular marker related to a sheep body length character, application of the SNP molecular marker and a kit for detecting the SNP molecular marker. The kit provided by the invention is used for performing polymorphism detection on the SNP molecular genetic marker, namely the 92690768th site of the No.4 chromosome, screening and determining the sheep body length character, and selecting the body length character to breed or assist to breed a long sheep individual with the body length character, so that a Tibetan sheep variety germplasm resource with high-quality body length character can be collected, and the kit has a very good application prospect.

The invention relates to the technical field of crop molecular genetic markers, and discloses an InDel molecular marker of avena nuda and application thereof.The molecular marker is a group of 314bp deletion variation and 2bp insertion variation in the 610439484-610440026 interval of the 5C chromosome position of a reference genome of an avena nuda variety OT3098, the nucleotide sequence of the deletion variation is shown as SEQ ID NO.1, the nucleotide sequence of the insertion variation is shown as SEQ ID NO.2, and the nucleotide sequence of the insertion variation is shown as SEQ ID NO.3. The nucleotide sequence of the insertion variation is 5 '-AA-3'. The molecular marker provided by the invention can realize stable detection in agarose gel electrophoresis by combining a specific primer pair (SEQ ID NO.2-3) with an optimized touchdown PCR (Polymerase Chain Reaction) program, has the advantage of being not influenced by a tissue part and a growth period, and provides an efficient and reliable technical means for oat germplasm identification, seed purity detection and molecular breeding.

The invention relates to an IGF2BP3 gene molecular marker primer related to pigeon weight traits and application of the IGF2BP3 gene molecular marker primer, and belongs to the technical field of biology. According to the invention, genome DNA of a to-be-detected pigeon is taken as a template, a specific primer is adopted to carry out PCR amplification on the genome DNA, then a PCR amplification product is subjected to Sanger sequencing and SNP molecular marker genotyping, and then pigeon body weight character selection is carried out. The weight of G / G genotype pigeons is higher than that of A / G and A / A genotype individuals, and the weight of A / G genotype pigeons is higher than that of A / A genotype individuals. By eliminating A / G and A / A genotype individuals and retaining G / G genotype individuals, the molecular marker has the beneficial effects that the molecular marker is used as a genetic marker for breeding pigeons, and the pigeons with large weight and uniformity are bred; the method can efficiently and quickly identify the weight traits of the pigeons, improves the uniformity of the weight of pigeons, provides a scientific basis for the early breeding of the pigeons, and better serves the breeding of the pigeons.

The invention discloses a primer composition and application of the primer composition in detection of animal bifidobacterium subsp. Lactis HN019. According to the present invention, bifidobacterium animalis subsp. Lactis HN019 is adopted as an object, bacterial strain specific genetic markers are screened through whole genome sequencing and single nucleotide polymorphism analysis, and a fluorescent quantitative PCR technology is combined so as to obtain the efficient, specific and stable HN019 quantitative detection primer composition and the method thereof; through system verification, the method is suitable for an HN019 strain level detection technical framework in scientific research and industrial application scenes, and also provides key technical support for market supervision and industrial healthy development of probiotic foods.

System and methods for alerting a healthcare provider to prescribed treatments having reduced or no effectiveness due to genetic composition is provided. A database containing treatments known to have reduced or no efficacy in persons having particular genetic markers is queried to determine whether any treatments prescribed by, or likely to be prescribed by, a healthcare provider to the patient are known to have reduced or no efficacy in persons having the same certain genetic markers as the patient. An alert indicating such information is displayed at a healthcare provider system.

The invention belongs to the technical field of livestock and poultry genetic markers and animal genetic breeding, and particularly relates to a structural variation molecular marker related to chicken secondary average feed intake and application of the structural variation molecular marker. The nucleotide sequence of the structural variation molecular marker provided by the invention is as shown in SEQ ID NO: 1, the structural variation molecular marker is located at 14th, 881, 990-14th, 882 and 295bp of a chicken chromosome 12, and the structural variation molecular marker is a deletion marker. The key genetic marker related to the chicken feeding behavior is provided, important information is provided for genetic breeding of the broiler chicken, meanwhile, broiler chicken varieties with different feeding behavior habits can be bred according to different markets and feeding environments, refined feeding management measures are formulated, and therefore the production efficiency and the feed utilization rate are improved, and the economic benefit is increased. And the economic benefit of the broiler industry is improved.

The invention provides a method and a system for detecting the chimeric rate of human cells based on a micro haploid, and belongs to the technical field of chimera analysis. According to the invention, the micro haploid is innovatively adopted as a basic unit of the genetic marker, and different from the prior art in which DNA fragments containing the genetic marker are generally enriched through a multiplex PCR amplification technology, a probe targeted capture technology is introduced for enrichment; the limitation of the multiple PCR amplification technology on the number of markers and the potential influence of the PCR amplification efficiency difference on accurate quantification are effectively avoided; meanwhile, the invention further develops a special data analysis algorithm which is used for processing sequencing data and accurately calculating the chimeric rate. A verification result shows that the scheme of the invention has excellent reliability and accuracy, and the detection sensitivity reaches the highest level of similar products on the current market. Therefore, the method has a wide application prospect in the fields needing micro chimera detection, such as hematopoietic stem cell transplantation, organ transplantation and the like.

The invention discloses a pig breeding screening method and system based on a body type database, and particularly relates to the technical field of pig breeding screening. The system comprises a multi-modal sign data acquisition and integration module for collecting and standardizing heterogeneous data sources such as phenotypic signs, genomics and environmental parameters of breeding pigs in real time, and a multi-dimensional biological characteristic modeling and analysis module for analyzing a nonlinear action mechanism among genetic markers, phenotypic characteristics and environmental factors, the genetic potential intelligent judgment module is used for quantitatively evaluating spatial expression potential and intergenerational transmission probability of genetic advantages of the breeding pigs through a deep learning algorithm; and the intelligent screening and dynamic monitoring module is used for establishing a multi-dimensional threshold dynamic regulation and control mechanism to realize accurate elimination decision and continuous optimization of a germplasm resource library. According to the method, the screening accuracy is remarkably improved by designing multi-source data fusion and online optimization capability, meanwhile, mistaken elimination is reduced through a redundant superior species temporary storage management module, and collaborative improvement of population genetic diversity and environmental adaptability is ensured.

The invention relates to a primer of an RBP4 gene molecular marker related to pig weak litter number character and application of the primer, and belongs to the technical field of biology. The RBP4 gene molecular marker is located at the 105043789th base of chromosome 14 of a pig reference genome Sscrofa11.1 version, the base is mutated into A or G, and the genotypes are A / A, A / G and G / G. The number of weak piglets of the G / G genotype pigs is lower than that of the A / G and A / A genotype individuals, and the number of weak piglets of the A / G genotype pigs is lower than that of the A / A genotype individuals. According to the present invention, the forward and reverse primer pair is used to amplify the fragment containing the molecular marker site, and the amplification product is subjected to Sanger sequencing so as to rapidly and accurately identify the genotype of the individual; the molecular marker is used as a genetic marker for pig breeding to breed pigs with less weak piglets; the method can efficiently and accurately detect the weak piglet number character, improves the survival rate of healthy piglets of a pig herd, and has an important value for breeding and production of pigs.

The invention discloses a 26 Multi-InDel genetic marker system for highly degraded sample typing, a detection primer and application of the 26 Multi-InDel genetic marker system, and belongs to the technical field of forensic medicine identification. According to the invention, through bioinformatics screening and experimental verification, a multi-InDel genetic marker system of which the lengths of 26 amplicons do not exceed 125 bp is constructed. The problem that degradation detection material detection and high system efficiency are difficult to consider at the same time is solved. The random matching probability of the system in Han population in Hunan reaches 2.25 * 10 <-17 >, the cumulative paternity exclusion rate is 0.999925, complete typing of highly degraded DNA can be achieved, triad paternity test can be completed, and a new technical scheme which is efficient and compatible with a capillary electrophoresis platform is provided for forensic practice.

The invention relates to a primer of a GAREM1 gene molecular marker related to the average daily gain character of pigs and application of the primer, and belongs to the technical field of biology. The GAREM1 gene molecular marker is located at the 116131455th basic group of chromosome 6 of a pig reference genome Sscrofa11.1 version, the basic group is mutated into A, and the genotypes are A / A and A / T. The average daily gain of A / T genotype pigs is higher than that of A / A genotype individuals. According to the present invention, the forward and reverse primer pair is used to amplify the fragment containing the molecular marker site, and the amplification product is subjected to Sanger sequencing so as to rapidly and accurately identify the genotype of the individual; the molecular marker can be used as a genetic marker for pig breeding to breed pigs with proper weight; the method can be used for efficiently and accurately detecting the average daily gain character of the pigs, and has an important value for breeding and production of the pigs.

The present invention relates to assays, methods, and kits to assess traumatic brain injury (TBI) in a subject. The present invention also relates to ultrasensitive assays for GFAP, tau, CKBB, IL-1β, IL-2, IL-6, IL-10, IL-22, IP-10, TNFα, TSLP, NFL, and / or NFH.

The invention relates to the technical field of crop molecular genetic markers, and discloses an Indel molecular marker of avena nuda and application of the molecular marker, the molecular marker is insertion variation of 551bp located at the 4D chromosome position 451669339-451669889 of a reference genome of an avena nuda variety Sanfensan, and the insertion sequence is as shown in SEQ ID NO. 1. The Indel molecular marker provided by the invention is completely related to the naked oat skin character, can be efficiently used for naked oat germplasm resource identification, variety purity detection and molecular marker-assisted breeding, remarkably improves the screening efficiency of naked oat excellent germplasm, and provides a brand new molecular detection technology for genetic improvement of the naked oat skin character. According to the invention, not only is a rapid and accurate technical standard established for naked oat variety identification, but also important molecular marker resources and application schemes are provided for oat quality improvement and germplasm innovation.

The invention discloses a molecular genetic marker YZU-GOOSE-OE-1 for a goose eye opening character and an application of the molecular genetic marker YZU-GOOSE-OE-1. The molecular genetic marker comprises a GG genotype and / or a TT genotype, and the molecular genetic marker of the GG genotype is a sequence located at the SNP1 site of the FREM1 gene; the molecular genetic marker of the TT genotype is a sequence located at the SNP3 site of the PTPRM gene; the login number of the FREM1 gene is NC089912, and the login number of the FREM1 gene is NC089912; and the login number of the PTPRM gene is NC089874. According to the method, the selection accuracy of the goosefoot phenotype of the goosefoot is improved, the breeding population scale is reduced and the breeding cost is reduced by utilizing the molecular genetic marker obviously associated with the goosefoot character, and on the basis, the sex-associated genetic characteristic of the goosefoot character is utilized to cultivate the goosefoot character auto-sex specialized line, so that compared with phenotype selection, the cultivation process is obviously accelerated.

The invention provides a DGAT1 gene SNPs marker for detecting sheep milk production traits and application thereof.Nucleotide sequence variation sites of the DGAT1 gene are detected with the first-filial generation of Dongfudrisheng sheep male * Hu sheep female as an object, a general linear mixing effect model is constructed, the correlation between nucleotide sequence variation and sheep milk production traits is researched, and the DGAT1 gene SNPs marker for detecting the sheep milk production traits is obtained. And digging a molecular genetic marker for regulating and controlling the lactation character of the sheep. Three SNPs (single nucleotide polymorphisms) sites are found in total; wherein two variation sites in the first intron are named as c.191 + 411 Cgt, and the second intron is named as c.191 + 411 Cgt; t (SNP1) and c.192-440 Cgt, and c. 292-440 Cgt; t (SNP2) and a variation site in the 17th exon are named as c.1461 Cgt; t (SNP3). For the SNP1 site, the average daily milk yield of the CT genotype ewe is increased by 8.2% and 17.04% compared with that of the CC genotype ewe and that of the TT genotype ewe respectively; for the SNP3 site, the milk fat percentage of the CC genotype ewe is improved by 0.314% compared with that of the CT genotype ewe. A new molecular marker can be provided for selection and improvement of lactation traits of the milk sheep, and the molecular marker has important application prospects in sheep screening or breeding.

The invention relates to the technical field of biology, and provides a genetic marker for children nephrotic syndrome genetic risk assessment. The invention relates to genetic detection and application of hormone sensitive nephrotic syndrome (pSSNS) of children. Nine risk sites, including new sites of 1q23.1, 1p36.13, 5p13.2, 10q21.3, 10q24.1 and the like, highly related to diseases are found by integrating whole genome association research (GWAS) data, combining Meta analysis and a conjugate false discovery rate (conjFDR) method and taking genetic information of IgA nephropathy as assistance. Research results show that genes near the loci have differential expression in pSSNS and IgAN patients, which prompts that the genes play an important role in the occurrence and development of diseases. The invention provides a molecular detection method based on the risk site, which can be used for risk assessment, auxiliary diagnosis and prognosis prediction of children's nephropathy. Meanwhile, the invention provides potential application values of the loci and related genes thereof in individualized medication and targeted therapy.

The invention relates to the technical field of preoperative risk prediction, in particular to a risk prediction method and system for an AI preoperative simulation operation. The method comprises the following steps: acquiring multi-modal medical data of a patient, and performing quantitative analysis to obtain structural characteristics of a pathological part of the patient, a genetic marker of the patient and phenotypic characteristics of the patient; carrying out multi-modal deep feature fusion based on the structural features of the pathological part of the patient, the genetic marker of the patient and the phenotypic features, and constructing a personalized patient digital model; a patient operation log is obtained, multi-stage operation step analysis and deep risk factor prediction are carried out, and patient personalized risk factors are generated; self-adaptive operation path planning and step-by-step sudden execution prediction are carried out based on patient operation logs, and risk prediction points of different operation paths are obtained. According to the invention, through efficient and accurate preoperative risk prediction, the safety of the operation is visually displayed.

Provided herein are improved methods and systems for detecting AMR genetic markers using multi-step sequencing assays. Also disclosed are methods and systems for preparing samples for use in high-throughput sequencing assays to detect AMR genetic markers.

The invention relates to a primer of a KLHL14 gene molecular marker related to pig weak litter number character and application of the primer, and belongs to the technical field of biology. The KLHL14 gene molecular marker is located at the 116519104 base of chromosome 6 of a pig reference genome Sscrofa11.1 version, the base is mutated into C or T, and the genotypes are C / C, C / T and T / T. According to the present invention, the forward and reverse primer pair is used to amplify the fragment containing the molecular marker site, and the amplification product is subjected to Sanger sequencing so as to rapidly and accurately identify the genotype of the individual; the molecular marker can be used as a genetic marker for pig breeding to breed pigs with less weak piglets; the method can efficiently and accurately detect the character of the number of the weak piglets of the pigs, improves the survival rate of healthy piglets of a swinery, and has an important value for breeding and production of the pigs.

The invention discloses an astragalus sinicus breeding genetic data mining system based on bioinformatics, and particularly relates to the technical field of data mining. The method comprises the following steps: acquiring and synchronizing original sequencing data of an astragalus sinicus host genome and a rhizobium symbiotic microorganism genome to generate a standardized genetic information data set; the method comprises the following steps: identifying sequence cross contamination sites between a host genome and a symbiotic microorganism genome, and eliminating genetic information interference of a microorganism source, so as to obtain purified host genome data without microorganism interference; analyzing the contribution degree of the microbial genome to the target character of the astragalus sinicus to obtain a microbial effect weight; integrating the purified host genome data and the microbial effect weight, constructing an astragalus sinicus character association analysis model, and positioning character association sites; and finally, screening candidate breeding markers based on character associated site information to obtain a precise breeding genetic marker set. According to the method, the accuracy and efficiency of milk vetch breeding are improved.

Provided in the present invention are the use of insertion / deletion polymorphism genetic markers in detecting the copy number of human chromosomal subtelomeric regions, a reagent panel for detecting the insertion / deletion polymorphisms genetic markers, and a method for detecting the copy number of human chromosomal subtelomeric regions.

The present invention provides an application of a SNP genetic marker affecting chicken breast width in chicken genetic breeding, belonging to the fields of livestock and poultry genomic selective breeding and biotechnology. The SNP genetic marker affecting chicken breast width includes CB_tag11, which corresponds to the physical position 17298459 of chromosome 11 of the chicken reference genome bGalGal 1.mat.broi ler.GRCg7b version sequence information published by NCBI, and belongs to the 14th intron of the gene GSE1, where the base is A or G. CB_tag11 helps to genetically increase breast width. Its application in chicken genetic breeding is beneficial for improving breast width in laying hens and obtaining high-quality laying hen varieties.

The present invention discloses a SNP locus combination for sheep genotyping, a liquid-phase chip and an application thereof, belonging to the technical field of molecular breeding. The present invention provides a SNP locus combination for sheep genotyping, and the SNP locus combination comprises 58,859 SNP loci. The SNP locus combination of the present invention covers multiple important economic traits, and encompasses important genetic loci related to the production performance, reproductive performance, disease resistance traits and adaptability of sheep, which helps to improve the breeding efficiency and production performance. By combining functional locus selection and liquid-phase chip capture sequencing technology, the present invention designs an efficient, low-cost and high-throughput 50K SNP liquid-phase chip for the whole genome of sheep, which can accurately screen genetic markers related to important economic traits of sheep, and provides a reliable molecular marker tool for the genome-wide association analysis, genomic selection, genetic improvement and phenotypic regulation of sheep.

The invention relates to the technical field of forensic genomics, in particular to a primer group and a kit for detecting genetic markers of 365 Y chromosomes and application of the primer group and the kit. According to the invention, an amplification primer group for specific 57 Y-STRs and 308 Y-SNPs of Chinese population is optimally designed, and a joint detection method with high sensitivity and high resolution is constructed. Y-STR haplotype analysis and Y-SNP haplogroup accurate typing can be synchronously realized, and the problems of high family checking false positive rate, insufficient geographic ancestor inference resolution and the like caused by single site type detection in the traditional technology are solved. The method is suitable for diversified biological samples such as blood, seminal stains and saliva, and provides a novel technical scheme of high-throughput, low-cost and multi-dimensional paternal genetic information analysis for forensic practice. Comprising construction of a Y-STR and Y-SNP composite amplification system based on next-generation sequencing and application of the Y-STR and Y-SNP composite amplification system in forensic medicine individual recognition, family investigation and biogeography ancestor inference.

The invention discloses a primer group, a kit and a detection method for simultaneously realizing species identification and individual identification, and belongs to the technical field of forensic identification. The invention discloses a primer group for simultaneously realizing species identification and individual identification. The primer group comprises a primer sequence for amplifying 11 animal characteristic sequences and a primer sequence for amplifying 30 individual genetic markers STR. The invention also discloses a kit comprising the primer group, and a detection method for species identification and individual recognition by adopting the primer group. The method is suitable for trace detection materials, and is high in detection speed, low in detection cost and high in accuracy.

Determining whether a particular sample is what it is labeled or sold as is an important concept, particularly to those who buy or sell food products. Particularly for end users, a fast, efficient, accurate way of determining whether a sample is being accurately marketed and sold is necessary. This invention allows a user to rapidly determine if a product is from a certain species or not, based on genetic markers for that product.

The invention discloses a gene marker for diagnosing latent tuberculosis infection and application of the gene marker, and belongs to the technical field of biological medicine. According to the present invention, the latent tuberculosis infection early diagnosis marker is screened based on the peripheral blood whole genome DNA methylation map, the new target is provided for the diagnosis of latent tuberculosis infection, the diagnosis efficiency of the marker is evaluated through pyrosequencing, the corresponding detection kit and the use method are developed, and the rapid and accurate diagnosis of latent tuberculosis infection is achieved.

The application provides application of a SNP genetic marker affecting chicken body weight at the onset of lay in genetic breeding of laying hens, and belongs to the field of animal genetic breeding and biotechnology.The SNP genetic marker affecting chicken body weight at the onset of lay comprises bw1egg_1 and / or bw1egg_2; the Ensembl number of the bw1egg_1 is rs318020581, corresponding to the 76311052th position of the positive strand of chromosome 4 in the chicken reference genome bGalGal1.mat.broiler.GRCg7b sequence published in NCBI, belonging to the 3rd intron of the gene C1QTNF7, and the base at the position is T or G; the Ensembl number of the bw1egg_2 is rs313708699, corresponding to the 74731889th position of the positive strand of chromosome 4 in the chicken reference genome bGalGal1.mat.broiler.GRCg7b sequence published in NCBI, belonging to the 8th intron of the gene SLIT2, and the base at the position is T or C.Both the bw1egg_1 and the bw1egg_2 are helpful to genetically improve the body weight at the onset of lay, and when applied to genetic breeding of chickens, are favorable to improving the body weight at the onset of lay of the laying hens and obtaining a laying hen breed with excellent performance in uniformity of the body weight at the onset of lay.

The invention discloses an SNP (Single Nucleotide Polymorphism) genetic marker associated with the number of live piglets born by sows and application thereof, and belongs to the technical field of pig genetic marker detection, the SNP genetic marker is located at a 213444403 bp nucleotide site of a first chromosome of a reference sequence of an international pig genome version 11.1, the basic group of the site is G or A, and the SNP genetic marker is located at the upstream 38945 bp position of a PTPRD gene. The invention also discloses an application of the SNP genetic marker in breeding of sows with high live piglet number. The SNP genetic marker can assist in increasing the number of live piglets born by the sow, provides a new marker resource for assisted selection of breeding sows, and can be used for genetic breeding of the breeding sows.