438 results about "Genetic marker" patented technology

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to growth traits of litopenaeus vannamei and application of the SNP molecular marker, and belongs to the technical field of biology. The SNP molecular markers 1-3 are remarkably related to the growth traits of the litopenaeus vannamei, genomic DNA of muscular tissue of the litopenaeus vannamei to be detected is extracted and then serves as template DNA to be subjected to PCR amplification and sequencing, the genotypes of the three molecular markers are determined, and the litopenaeus vannamei with obvious growth advantages can be rapidly screened out. The molecular marker can accelerate the breeding process of the improved variety of the litopenaeus vannamei, and provides a theoretical basis for mining application of related genetic markers in litopenaeus vannamei molecular marker-assisted breeding.

The invention relates to a pigeon individual typing detection system, a kit and application thereof. The kit comprises a pigeon individual typing detection system, the pigeon individual typing detection system comprises a primer combination and a composite amplification premix, and the primer combination comprises primers for amplifying 23 STR (short tandem repeat) microsatellite markers, bird sex detection CHD gene loci and 5 SNP (single nucleotide polymorphism) genetic markers. The pigeon individual typing detection system and the kit provided by the invention can realize single-tube detection of 29 pigeon DNA genetic markers, comprise STR genetic markers for realizing pigeon individual identification and parent-child relationship identification and single nucleotide polymorphism genetic markers for evaluating the flying ability of pigeons, and are a detection system which can be detected at present and contains the maximum number of pigeon DNA gene loci; the method has a good application prospect in the fields of pigeon identity identification, parent-child relationship identification and flight ability evaluation.

The invention belongs to the field of SNP (Single Nucleotide Polymorphism) detection, and particularly provides an SNP molecular genetic marker related to a sheep horn length character and a kit for detecting the SNP molecular marker. The kit provided by the invention is used for performing polymorphism detection on the SNP molecular genetic marker, namely the 30991957th site of the 10th chromosome, screening and determining the horn length character of the sheep, and selecting the horn length character to breed or assist in breeding horn-free sheep individuals, so that the collection of high-quality horn-free Tibetan sheep variety germplasm resources can be ensured, and the kit has a very excellent application prospect.

The invention relates to an STON1 gene molecular marker primer related to chicken double-yolk egg traits and application of the STON1 gene molecular marker primer, and belongs to the technical field of biology. The STON1 gene molecular marker is a 7867409th basic group of a chicken reference genome GRCg7b version chromosome 3, the basic group is mutated into A or G, and the genotypes are A / A, A / G and G / G. The double-yolk egg ratio of the G / G genotype chicken is higher than that of the A / G and A / A genotype individuals, and the double-yolk egg ratio of the A / G genotype chicken is higher than that of the A / A genotype individuals. A forward and reverse primer pair is used for amplifying a fragment containing the molecular marker site, Sanger sequencing is carried out on an amplification product, the genotype of an individual is rapidly and accurately identified to serve as a genetic marker for chicken breeding, and the chicken with the higher proportion of double-yolk eggs is bred; the double-yolk egg traits can be efficiently and accurately detected, the breeding accuracy and breeding efficiency of the chicken double-yolk egg proportion are improved, and the method has important value for chicken breeding and production.

The invention belongs to the field of SNP (Single Nucleotide Polymorphism) detection, and particularly provides an SNP molecular marker related to a sheep horn length character and a kit for detecting the SNP molecular marker. The kit provided by the invention is used for performing polymorphism detection on the SNP molecular genetic marker, namely the 30947874th site of the 10th chromosome, screening and determining the horn length character of the sheep, and selecting the horn length character to breed or assist in breeding horn-free sheep individuals, so that the collection of high-quality horn-free Tibetan sheep variety germplasm resources can be ensured, and the kit has a very excellent application prospect.

The invention provides a crop whole genome prediction method and system based on machine learning, and the method comprises the steps: firstly obtaining a gene expression data set of a target crop in a plurality of growth cycles, the gene expression data set containing a plurality of gene sequence samples composed of genetic markers and phenotypic character data; performing feature extraction on the gene expression data set to obtain gene association features and growth character features, performing fusion prediction on the gene association features and the growth character features by using a preset machine learning model to generate fusion prediction features, and determining a whole genome prediction result according to the fusion prediction features. The whole genome prediction result can indicate character expression trends of crops in different environments, and finally, an adaptability optimization strategy is generated based on the whole genome prediction result and fed back to a crop cultivation system, cultivation parameter adjustment is triggered, crop cultivation precision and intelligence are achieved, and the cultivation efficiency and crop adaptability are improved.

The invention belongs to the field of SNP (Single Nucleotide Polymorphism) detection, and particularly provides an SNP molecular marker related to a sheep body length character, application of the SNP molecular marker and a kit for detecting the SNP molecular marker. The kit provided by the invention is used for performing polymorphism detection on the SNP molecular genetic marker, namely the 92690768th site of the No.4 chromosome, screening and determining the sheep body length character, and selecting the body length character to breed or assist to breed a long sheep individual with the body length character, so that a Tibetan sheep variety germplasm resource with high-quality body length character can be collected, and the kit has a very good application prospect.

The present disclosure describes techniques for generating a sepsis mortality prediction model. The sepsis mortality prediction model can predict tire likelihood of sepsis mortality for a subject. The sepsis mortality prediction model can be generated by using feature selection to select a model subset of genetic marker(s) from training data. Candidate sepsis mortality prediction models can be trained using the model subset. The sepsis mortality prediction model can be selected from the candidate prediction models based on determining a performance metric associated with each candidate prediction using a test subset of the training data.

The invention relates to the technical field of crop molecular genetic markers, and discloses an InDel molecular marker of avena nuda and application thereof.The molecular marker is a group of 314bp deletion variation and 2bp insertion variation in the 610439484-610440026 interval of the 5C chromosome position of a reference genome of an avena nuda variety OT3098, the nucleotide sequence of the deletion variation is shown as SEQ ID NO.1, the nucleotide sequence of the insertion variation is shown as SEQ ID NO.2, and the nucleotide sequence of the insertion variation is shown as SEQ ID NO.3. The nucleotide sequence of the insertion variation is 5 '-AA-3'. The molecular marker provided by the invention can realize stable detection in agarose gel electrophoresis by combining a specific primer pair (SEQ ID NO.2-3) with an optimized touchdown PCR (Polymerase Chain Reaction) program, has the advantage of being not influenced by a tissue part and a growth period, and provides an efficient and reliable technical means for oat germplasm identification, seed purity detection and molecular breeding.

The invention relates to an IGF2BP3 gene molecular marker primer related to pigeon weight traits and application of the IGF2BP3 gene molecular marker primer, and belongs to the technical field of biology. According to the invention, genome DNA of a to-be-detected pigeon is taken as a template, a specific primer is adopted to carry out PCR amplification on the genome DNA, then a PCR amplification product is subjected to Sanger sequencing and SNP molecular marker genotyping, and then pigeon body weight character selection is carried out. The weight of G / G genotype pigeons is higher than that of A / G and A / A genotype individuals, and the weight of A / G genotype pigeons is higher than that of A / A genotype individuals. By eliminating A / G and A / A genotype individuals and retaining G / G genotype individuals, the molecular marker has the beneficial effects that the molecular marker is used as a genetic marker for breeding pigeons, and the pigeons with large weight and uniformity are bred; the method can efficiently and quickly identify the weight traits of the pigeons, improves the uniformity of the weight of pigeons, provides a scientific basis for the early breeding of the pigeons, and better serves the breeding of the pigeons.

The invention discloses a primer composition and application of the primer composition in detection of animal bifidobacterium subsp. Lactis HN019. According to the present invention, bifidobacterium animalis subsp. Lactis HN019 is adopted as an object, bacterial strain specific genetic markers are screened through whole genome sequencing and single nucleotide polymorphism analysis, and a fluorescent quantitative PCR technology is combined so as to obtain the efficient, specific and stable HN019 quantitative detection primer composition and the method thereof; through system verification, the method is suitable for an HN019 strain level detection technical framework in scientific research and industrial application scenes, and also provides key technical support for market supervision and industrial healthy development of probiotic foods.

The invention relates to a CCDC171 gene molecular marker primer related to egg weight character and application of the CCDC171 gene molecular marker primer, and belongs to the technical field of biology. The CCDC171 gene molecular marker is located at the 32551904 base of a Z chromosome of a chicken reference genome GRCg7b version, the base is mutated into C or T, and the genotypes are C / C and T / T. And the egg weight of the C / C genotype chicken is higher than that of the T / T genotype individual. According to the present invention, the forward and reverse primer pair is used to amplify the fragment containing the molecular marker site, and the amplification product is subjected to Sanger sequencing so as to rapidly and accurately identify the genotype of the individual; the molecular marker can be used as a genetic marker for chicken breeding to breed chicken with proper egg weight; the egg weight character can be efficiently and accurately detected, the uniformity of the egg weight of chicken flocks is improved, and the method has important value for chicken breeding and production.

System and methods for alerting a healthcare provider to prescribed treatments having reduced or no effectiveness due to genetic composition is provided. A database containing treatments known to have reduced or no efficacy in persons having particular genetic markers is queried to determine whether any treatments prescribed by, or likely to be prescribed by, a healthcare provider to the patient are known to have reduced or no efficacy in persons having the same certain genetic markers as the patient. An alert indicating such information is displayed at a healthcare provider system.

The invention belongs to the technical field of livestock and poultry genetic markers and animal genetic breeding, and particularly relates to a structural variation molecular marker related to chicken secondary average feed intake and application of the structural variation molecular marker. The nucleotide sequence of the structural variation molecular marker provided by the invention is as shown in SEQ ID NO: 1, the structural variation molecular marker is located at 14th, 881, 990-14th, 882 and 295bp of a chicken chromosome 12, and the structural variation molecular marker is a deletion marker. The key genetic marker related to the chicken feeding behavior is provided, important information is provided for genetic breeding of the broiler chicken, meanwhile, broiler chicken varieties with different feeding behavior habits can be bred according to different markets and feeding environments, refined feeding management measures are formulated, and therefore the production efficiency and the feed utilization rate are improved, and the economic benefit is increased. And the economic benefit of the broiler industry is improved.

The present invention relates to the field of nutrition and health management. An exemplary method for generating a personalized nutritional plan for a user comprises: receiving, from the user, a natural-language query; identifying, based on the natural-language query, a task and one or more parameters; retrieving from one or more databases, based on the task and the one or more parameters, one or more genetic markers associated with the user, and one or more traits associated with the user; constructing one or more data structures associated with the task based on the one or more genetic markers and the one or more traits associated with the user; inputting the one or more data structures into a trained language model to generate the personalized nutritional plan; and displaying the personalized nutritional plan.

The invention relates to a PPP3R1 gene molecular marker primer related to the size character of a cockscomb and application of the PPP3R1 gene molecular marker primer, and belongs to the technical field of biology. The molecular marker of the PPP3R1 gene is located at the 11347456 base of chromosome 3 of a chicken reference genome GRCg7b version, the base is mutated into T or G, and the genotypes are G / G, T / G and T / T. The cockscombs of the G / G genotype chickens are larger than those of the T / G and T / T genotype individuals, and the cockscombs of the T / G genotype chickens are larger than those of the T / T genotype individuals. According to the present invention, the forward and reverse primer pair is used to amplify the fragment containing the molecular marker site, and the amplification product is subjected to Sanger sequencing so as to rapidly and accurately identify the genotype of the individual; the molecular marker can be used as a genetic marker for chicken breeding to breed chicken with proper cockscomb size; the method can efficiently and accurately detect the size of the cockscomb of the chicken, improves the uniformity of the cockscomb of a group, and has important value for breeding and production of the chicken.

The invention provides a method and a system for detecting the chimeric rate of human cells based on a micro haploid, and belongs to the technical field of chimera analysis. According to the invention, the micro haploid is innovatively adopted as a basic unit of the genetic marker, and different from the prior art in which DNA fragments containing the genetic marker are generally enriched through a multiplex PCR amplification technology, a probe targeted capture technology is introduced for enrichment; the limitation of the multiple PCR amplification technology on the number of markers and the potential influence of the PCR amplification efficiency difference on accurate quantification are effectively avoided; meanwhile, the invention further develops a special data analysis algorithm which is used for processing sequencing data and accurately calculating the chimeric rate. A verification result shows that the scheme of the invention has excellent reliability and accuracy, and the detection sensitivity reaches the highest level of similar products on the current market. Therefore, the method has a wide application prospect in the fields needing micro chimera detection, such as hematopoietic stem cell transplantation, organ transplantation and the like.

The invention discloses a pig breeding screening method and system based on a body type database, and particularly relates to the technical field of pig breeding screening. The system comprises a multi-modal sign data acquisition and integration module for collecting and standardizing heterogeneous data sources such as phenotypic signs, genomics and environmental parameters of breeding pigs in real time, and a multi-dimensional biological characteristic modeling and analysis module for analyzing a nonlinear action mechanism among genetic markers, phenotypic characteristics and environmental factors, the genetic potential intelligent judgment module is used for quantitatively evaluating spatial expression potential and intergenerational transmission probability of genetic advantages of the breeding pigs through a deep learning algorithm; and the intelligent screening and dynamic monitoring module is used for establishing a multi-dimensional threshold dynamic regulation and control mechanism to realize accurate elimination decision and continuous optimization of a germplasm resource library. According to the method, the screening accuracy is remarkably improved by designing multi-source data fusion and online optimization capability, meanwhile, mistaken elimination is reduced through a redundant superior species temporary storage management module, and collaborative improvement of population genetic diversity and environmental adaptability is ensured.

The invention relates to a primer of an RBP4 gene molecular marker related to pig weak litter number character and application of the primer, and belongs to the technical field of biology. The RBP4 gene molecular marker is located at the 105043789th base of chromosome 14 of a pig reference genome Sscrofa11.1 version, the base is mutated into A or G, and the genotypes are A / A, A / G and G / G. The number of weak piglets of the G / G genotype pigs is lower than that of the A / G and A / A genotype individuals, and the number of weak piglets of the A / G genotype pigs is lower than that of the A / A genotype individuals. According to the present invention, the forward and reverse primer pair is used to amplify the fragment containing the molecular marker site, and the amplification product is subjected to Sanger sequencing so as to rapidly and accurately identify the genotype of the individual; the molecular marker is used as a genetic marker for pig breeding to breed pigs with less weak piglets; the method can efficiently and accurately detect the weak piglet number character, improves the survival rate of healthy piglets of a pig herd, and has an important value for breeding and production of pigs.

The invention discloses a 26 Multi-InDel genetic marker system for highly degraded sample typing, a detection primer and application of the 26 Multi-InDel genetic marker system, and belongs to the technical field of forensic medicine identification. According to the invention, through bioinformatics screening and experimental verification, a multi-InDel genetic marker system of which the lengths of 26 amplicons do not exceed 125 bp is constructed. The problem that degradation detection material detection and high system efficiency are difficult to consider at the same time is solved. The random matching probability of the system in Han population in Hunan reaches 2.25 * 10 <-17 >, the cumulative paternity exclusion rate is 0.999925, complete typing of highly degraded DNA can be achieved, triad paternity test can be completed, and a new technical scheme which is efficient and compatible with a capillary electrophoresis platform is provided for forensic practice.

The invention discloses a kit for detecting micro-haplotype and STR gene loci of normal, Y and X chromosomes based on a next-generation sequencing technology and a primer combination of the kit. The primer combination provided by the invention consists of 738 types of primers which are sequentially shown as SEQ ID NO: 1 to SEQ ID NO: 738. According to the invention, a multi-genetic marker composite detection system containing autosomal STR gene loci, X-STR gene loci, Y-STR gene loci and micro-haplotype gene loci is constructed, and 378 gene loci, namely 55 autosomal STR gene loci, 96 Y-chromosome STR gene loci, 30 X-STR gene loci, 196 micro-haplotype gene loci and 1 gender determination gene locus, can be detected at a time. The method can support forensic medicine applications such as individual identification, paternity test, fine checking of male families, mixed DNA splitting, complex genetic relationship analysis and micro-degradation sample inspection, and provides powerful evidences for practical applications such as case reconnaissance.

The invention provides an application of a genetic marker associated with a chest width character in an RNASEH2B gene in chicken genetic breeding, and belongs to the technical field of chicken genetic breeding and biology.The genetic marker associated with the chest width character in the RNASEH2B gene comprises CBmark er1, the CBmark er1 corresponds to the 169817100th site of the physical position of a first chromosome of chicken reference genome bGa < 1 >. Mat.broi ler.GRCg7b version sequence information published in NCBI, and the CBmark er1 corresponds to the 169817100th site of the physical position of a second chromosome of the chicken reference genome bGa < 1 >. Mat.broi ler.GRCg7b version sequence information. The nucleotide sequence belongs to the ninth intron sequence of the gene RNASEH2B, and the basic group in the ninth intron sequence is G or A; the CBmarker1 contributes to heredity improvement of the chest breadth, and when the CBmarker1 is applied to genetic breeding of chickens, improvement of the chest breadth is facilitated, and a high-quality laying hen variety is obtained.

The invention relates to a primer of a GAREM1 gene molecular marker related to the average daily gain character of pigs and application of the primer, and belongs to the technical field of biology. The GAREM1 gene molecular marker is located at the 116131455th basic group of chromosome 6 of a pig reference genome Sscrofa11.1 version, the basic group is mutated into A, and the genotypes are A / A and A / T. The average daily gain of A / T genotype pigs is higher than that of A / A genotype individuals. According to the present invention, the forward and reverse primer pair is used to amplify the fragment containing the molecular marker site, and the amplification product is subjected to Sanger sequencing so as to rapidly and accurately identify the genotype of the individual; the molecular marker can be used as a genetic marker for pig breeding to breed pigs with proper weight; the method can be used for efficiently and accurately detecting the average daily gain character of the pigs, and has an important value for breeding and production of the pigs.

The invention relates to an SH3GL3 gene molecular marker primer related to egg weight character and application of the SH3GL3 gene molecular marker primer, and belongs to the technical field of biology. According to the present invention, the genome DNA of the chicken to be detected is adopted as the template, the specific primer is adopted to perform PCR amplification on the template, the PCR amplification product is subjected to Sanger sequencing and SNP molecular marker gene typing, and the 52-week-old egg weight character selection of the chicken can be achieved based on the genotype of the SNP molecular marker; by eliminating A / G and A / A genotype individuals and retaining G / G genotype individuals, the molecular marker has the beneficial effects that the molecular marker can be used as a genetic marker for chicken breeding to breed 52-week-old chickens which are relatively small and uniform in egg weight; the method can be used for efficiently and quickly identifying the egg weight character of the chickens at the age of 52 weeks, improves the uniformity of the egg weight of chicken flocks, provides a scientific basis for early breeding of the chickens, and has an important value for chicken breeding. In addition, the detection method disclosed by the invention is simple and easy to operate, and can be carried out in a laboratory.

The invention discloses an FABP3 gene SNPs marker for detecting sheep milk production traits and application of the FABP3 gene SNPs marker, and belongs to the technical field of biology. According to the invention, lactating sheep is taken as a research object, Sanger and other technologies are applied to detect a nucleotide sequence variation site of an FABP3 gene, a general linear mixing effect model is constructed, the correlation between nucleotide sequence variation and sheep lactation traits is researched, and a molecular genetic marker for regulating and controlling the sheep lactation traits is excavated; on the SNP1 site, the cream-fat percentages of AG and GG genotype individuals are respectively 0.99% and 1.01% higher than those of AA type individuals. On the site SNP2, the milk fat rates of TC and TT genotype individuals are 1.18% and 1.23% higher than those of CC type individuals respectively, and the daily lactation volumes of the TC and TT genotype individuals are 1.97% and 1.27% higher than those of the CC type individuals respectively.

The present invention relates to assays, methods, and kits to assess traumatic brain injury (TBI) in a subject. The present invention also relates to ultrasensitive assays for GFAP, tau, CKBB, IL-1β, IL-2, IL-6, IL-10, IL-22, IP-10, TNFα, TSLP, NFL, and / or NFH.

The invention belongs to the technical field of bioengineering, and particularly relates to a haplotype related to pig growth, meat production and feed conversion rate and breeding application. The genetic marker clones 22 loci from an lncMGPF gene promoter region of a pig No.8 chromosome. SNPs loci are typed through a direct sequencing technology, a linkage imbalance analysis result shows that 22 loci are all in a strong linkage imbalance state, haplotype analysis is further carried out, and a result shows that two main haplotypes are generated. Association analysis shows that compared with other combined haplotype individuals, the combined haplotype H1-H1 individuals have the characteristics that the day age time is short when the combined haplotype H1-H1 individuals reach 100 kg, the eye muscle thickness is large, and the average feed conversion ratio is small when the combined haplotype H1-H1 individuals are 30-100 kg. The invention provides a new marker for molecular breeding of pig meat traits.

The invention relates to the technical field of crop molecular genetic markers, and discloses an Indel molecular marker of avena nuda and application of the molecular marker, the molecular marker is insertion variation of 551bp located at the 4D chromosome position 451669339-451669889 of a reference genome of an avena nuda variety Sanfensan, and the insertion sequence is as shown in SEQ ID NO. 1. The Indel molecular marker provided by the invention is completely related to the naked oat skin character, can be efficiently used for naked oat germplasm resource identification, variety purity detection and molecular marker-assisted breeding, remarkably improves the screening efficiency of naked oat excellent germplasm, and provides a brand new molecular detection technology for genetic improvement of the naked oat skin character. According to the invention, not only is a rapid and accurate technical standard established for naked oat variety identification, but also important molecular marker resources and application schemes are provided for oat quality improvement and germplasm innovation.

The invention discloses a molecular genetic marker YZU-GOOSE-OE-1 for a goose eye opening character and an application of the molecular genetic marker YZU-GOOSE-OE-1. The molecular genetic marker comprises a GG genotype and / or a TT genotype, and the molecular genetic marker of the GG genotype is a sequence located at the SNP1 site of the FREM1 gene; the molecular genetic marker of the TT genotype is a sequence located at the SNP3 site of the PTPRM gene; the login number of the FREM1 gene is NC089912, and the login number of the FREM1 gene is NC089912; and the login number of the PTPRM gene is NC089874. According to the method, the selection accuracy of the goosefoot phenotype of the goosefoot is improved, the breeding population scale is reduced and the breeding cost is reduced by utilizing the molecular genetic marker obviously associated with the goosefoot character, and on the basis, the sex-associated genetic characteristic of the goosefoot character is utilized to cultivate the goosefoot character auto-sex specialized line, so that compared with phenotype selection, the cultivation process is obviously accelerated.

The invention provides a DGAT1 gene SNPs marker for detecting sheep milk production traits and application thereof.Nucleotide sequence variation sites of the DGAT1 gene are detected with the first-filial generation of Dongfudrisheng sheep male * Hu sheep female as an object, a general linear mixing effect model is constructed, the correlation between nucleotide sequence variation and sheep milk production traits is researched, and the DGAT1 gene SNPs marker for detecting the sheep milk production traits is obtained. And digging a molecular genetic marker for regulating and controlling the lactation character of the sheep. Three SNPs (single nucleotide polymorphisms) sites are found in total; wherein two variation sites in the first intron are named as c.191 + 411 Cgt, and the second intron is named as c.191 + 411 Cgt; t (SNP1) and c.192-440 Cgt, and c. 292-440 Cgt; t (SNP2) and a variation site in the 17th exon are named as c.1461 Cgt; t (SNP3). For the SNP1 site, the average daily milk yield of the CT genotype ewe is increased by 8.2% and 17.04% compared with that of the CC genotype ewe and that of the TT genotype ewe respectively; for the SNP3 site, the milk fat percentage of the CC genotype ewe is improved by 0.314% compared with that of the CT genotype ewe. A new molecular marker can be provided for selection and improvement of lactation traits of the milk sheep, and the molecular marker has important application prospects in sheep screening or breeding.