Forensic medicine compound detection kit based on Y chromosome SNP (single nucleotide polymorphism) genetic marker

A genetic marker, Y chromosome technology, applied in the field of forensic genetics, can solve the problems of difficult selection of Y chromosome SNP genetic markers, limited number of Y-SNPs, and no Y-SNP composite detection system, and achieves widespread promotion and application value. , accurate typing, high-efficiency effect

Active Publication Date: 2013-06-05
SICHUAN UNIV
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  • Abstract
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Problems solved by technology

However, compared with other chromosomes, the Y chromosome sequence has less variation and its distribution has obvious population differences, which makes the selection of Y chromosome SNP genetic markers with forensic value difficult.
In particular, the number of Y-SNPs in East Asian populations is currently limited, and there is no high-efficiency Y-SNP composite detection system that can be applied to Chinese Han populations

Method used

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  • Forensic medicine compound detection kit based on Y chromosome SNP (single nucleotide polymorphism) genetic marker
  • Forensic medicine compound detection kit based on Y chromosome SNP (single nucleotide polymorphism) genetic marker
  • Forensic medicine compound detection kit based on Y chromosome SNP (single nucleotide polymorphism) genetic marker

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Embodiment 1 Preparation of kit of the present invention

[0068] The Y chromosome SNP composite detection kit for detection may include the following reagents packaged separately:

[0069] a) Multiplex amplification primer mix. It was obtained by mixing the amplification primers shown in Table 1, and was synthesized by TaKaRa Biotechnology Company. The synthesized 20 pairs of amplification primers were prepared with ultrapure water to 50pM / μL, and then mixed according to the ratio in Table 6 to make a composite amplification primer. Primer mix.

[0070] b) Compound amplification reaction mixture. The PCR reaction mixture One shot La PCR of TaKaRa Biotechnology company is used in this embodiment TM Mix.

[0071] c) Primer mix for multiplex single base extension reactions. It was obtained by mixing the primers for the single-base extension reaction shown in Table 2, and was synthesized by TaKaRa Biotechnology Company. The synthesized 20 single-base extension react...

Embodiment 2

[0081] Example 2 Using the method of the present invention to detect unrelated Han nationality individual specimens

[0082] Use the kit in Example 1 to detect unrelated Han individuals.

[0083] a. Genomic DNA was extracted from blood samples of 220 unrelated Han individuals by the Chelex-100 method, and used as a template for multiplex amplification.

[0084] b. Using the DNA template in step a, use the composite amplification primer mixture and the composite amplification reaction mixture to perform composite PCR amplification on the sample DNA in the following amplification system.

[0085]

[0086]

[0087] Amplified Thermal Cycling Parameters

[0088]

[0089] Cycle from step 2 to step 4 34 times

[0090] 5. 72°C for 10 minutes

[0091] c. Purification of multiple PCR products, the following is the purification system of each sample amplification product

[0092]

[0093] Amplified product purification reaction conditions:

[0094] 37°C 60 minutes

[00...

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Abstract

The invention belongs to the field of forensic medicine genetics and in particular relates to a forensic medicine compound detection kit based on a Y chromosome SNP (single nucleotide polymorphism) genetic marker for individual recognition and genetic relationship identification by a legal medical expert. The forensic medicine compound detection kit provided by the invention is used for carrying out forensic medicine genetic relationship identification and individual recognition on human biology detection materials by utilizing a Y chromosome SNP genetic marker. According to the technical scheme for solving the technical problem, the forensic medicine compound detection kit based on the Y chromosome SNP genetic marker comprises a separated and packaged compound amplification primer mixture, a multiple single-basic-group extension reaction primer mixture, an allele typing standard mixture, a compound amplification reaction mixture and a single-basic-group extension reaction mixture. The kit provided by the invention can be applied to detection of common degradable materials in forensic medicine.

Description

technical field [0001] The invention belongs to the field of forensic genetics, in particular to a forensic compound detection kit based on Y chromosome SNP genetic markers for forensic individual identification and kinship identification. Background technique [0002] Single Nucleotide Polymorphism (SNP) is a DNA sequence polymorphism caused by a single base variation at a specific nucleotide position in the genome, and is the most common and widely distributed type of DNA polymorphism in the human genome . SNPs are mostly biallelic genetic markers, which have the characteristics of genetic stability, low mutation rate, small amplification fragments, easy automation, and high-throughput analysis. [0003] The human Y chromosome is the sex-determining chromosome unique to males. Due to the different ways of inheritance, the Y chromosome can be divided into two regions, one is the pseudo-autosomal region located at both ends of the Y chromosome, and the other is the Y-speci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 侯一平魏蔚颜静罗海玻张霁李英碧吴谨
Owner SICHUAN UNIV
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